subject:(caffeic acid)

University of the Western Cape

1. Klein, Ashwil Johan. Modulation of soybean and maize antioxidant activities by Caffeic acid and nitric oxide under salt stress .

Degree: 2012, University of the Western Cape

URL: http://hdl.handle.net/11394/4502

► This study explores the roles of exogenously applied nitric oxide, exogenously applied caffeic acid and salt stress on the antioxidant system in cereal (exemplified by… (more)

▼ This study explores the roles of exogenously applied nitric oxide, exogenously applied caffeic acid and salt stress on the antioxidant system in cereal (exemplified by maize) and legume (using soybean as an example) plants together with their influence on membrane integrity and cell death.This study investigates changes in H2O2 content, root lipid peroxidation, root cell death and antioxidant enzymatic activity in maize roots in response to exogenously applied nitric oxide (NO) and salt stress. This part of the study is based on the partially understood interaction between NO and reactive oxygen species (ROS) such as H2O2 and the role of antioxidant enzymes in plant salt stress responses. The results show that application of salt (NaCl) results in elevated levels of H2O2 and an increase in lipid peroxidation, consequently leading to increased cell death. The study also shows that by regulating the production and detoxification of ROS through modulation of antioxidant enzymatic activities, NO plays a pivotal role in maize responses to salt stress. The study argues for NO as a regulator of redox homeostasis that prevents excessive ROS accumulation during exposure of maize to salinity stress that would otherwise be deleterious to maize. This study extends the role of exogenously applied NO to improve salt stress tolerance in cereals crops (maize) further to its role in enhancing salt stress tolerance in legumes. The effect of long-term exposure of soybean to NO and salt stress on root nodule antioxidant activity was investigated to demonstrate the role of NO in salt stress tolerance. The results show that ROS scavenging antioxidative enzymes like SOD, GPX and GR are differentially regulated in response to exogenous application of NO and salt stress. It remains to be determined if the NOinduced changes in antioxidant enzyme activity under salt stress are sufficient to efficiently reduce ROS accumulation in soybean root nodules to levels close to those of unstressed soybean root nodules. Furthermore, this study investigates the effect of long-term exposure of soybean to exogenous caffeic acid (CA) and salt stress, on the basis of the established role of CA as an antioxidant and the involvement of antioxidant enzymes in plant salt stress responses. The effect of CA on soybean nodule number, biomass (determined on the basis of nodule dry weight, root dry weight and shoot dry weight), nodule NO content, and nodule cyclic guanosine monophosphate (cGMP) content in response to salt stress was investigated. Additionally, CA-induced changes in nodule ROS content, cell viability, lipid peroxidation and antioxidant enzyme activity as well as some genes that encode antioxidant enzymes were investigated in the presence or absence of salt stress. The study shows that long-term exposure of soybean to salt stress results in reduced biomass associated with accumulation of ROS, elevated levels of lipid peroxidation and elevated levels of cell death. However, exogenously applied CA reversed the negative effects of salt stress on soybean… Advisors/Committee Members: Ludidi, Ndomelele Ndiko (advisor), Keyster, Marshall (advisor).

Subjects/Keywords: Nitric oxide; Antioxidant enzymes; Caffeic acid; Salt stress tolerance

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APA (6^th Edition):

Klein, A. J. (2012). Modulation of soybean and maize antioxidant activities by Caffeic acid and nitric oxide under salt stress . (Thesis). University of the Western Cape. Retrieved from http://hdl.handle.net/11394/4502

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Klein, Ashwil Johan. “Modulation of soybean and maize antioxidant activities by Caffeic acid and nitric oxide under salt stress .” 2012. Thesis, University of the Western Cape. Accessed December 09, 2019. http://hdl.handle.net/11394/4502.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Klein, Ashwil Johan. “Modulation of soybean and maize antioxidant activities by Caffeic acid and nitric oxide under salt stress .” 2012. Web. 09 Dec 2019.

Vancouver:

Klein AJ. Modulation of soybean and maize antioxidant activities by Caffeic acid and nitric oxide under salt stress . [Internet] [Thesis]. University of the Western Cape; 2012. [cited 2019 Dec 09]. Available from: http://hdl.handle.net/11394/4502.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Klein AJ. Modulation of soybean and maize antioxidant activities by Caffeic acid and nitric oxide under salt stress . [Thesis]. University of the Western Cape; 2012. Available from: http://hdl.handle.net/11394/4502

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Iowa State University

2. Ye, Zhong. Metabolism of herbal phenolics in gut/oral microbiota or Caco-2 cells and bioavailability associated efficacy of caffeic acid in mouse colitis.

Degree: 2009, Iowa State University

URL: https://lib.dr.iastate.edu/etd/10363

► Phenolics, one category of micronutrients abundant in human diet, are believed to prevent some degenerative diseases such as cardiovascular diseases, digestive system diseases, and cancer.… (more)

Subjects/Keywords: Caco-2; Caffeic acid; Colitis; Metabolism; mRNA CYP4B1; Phenolic; Nutrition

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ye, Z. (2009). Metabolism of herbal phenolics in gut/oral microbiota or Caco-2 cells and bioavailability associated efficacy of caffeic acid in mouse colitis. (Thesis). Iowa State University. Retrieved from https://lib.dr.iastate.edu/etd/10363

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ye, Zhong. “Metabolism of herbal phenolics in gut/oral microbiota or Caco-2 cells and bioavailability associated efficacy of caffeic acid in mouse colitis.” 2009. Thesis, Iowa State University. Accessed December 09, 2019. https://lib.dr.iastate.edu/etd/10363.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ye, Zhong. “Metabolism of herbal phenolics in gut/oral microbiota or Caco-2 cells and bioavailability associated efficacy of caffeic acid in mouse colitis.” 2009. Web. 09 Dec 2019.

Vancouver:

Ye Z. Metabolism of herbal phenolics in gut/oral microbiota or Caco-2 cells and bioavailability associated efficacy of caffeic acid in mouse colitis. [Internet] [Thesis]. Iowa State University; 2009. [cited 2019 Dec 09]. Available from: https://lib.dr.iastate.edu/etd/10363.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ye Z. Metabolism of herbal phenolics in gut/oral microbiota or Caco-2 cells and bioavailability associated efficacy of caffeic acid in mouse colitis. [Thesis]. Iowa State University; 2009. Available from: https://lib.dr.iastate.edu/etd/10363

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Iowa State University

3. Qiang, Zhiyi. Bioavailability and metabolism of botanical constituents and enhancement of intestinal barrier function by caffeic acid derivatives in Caco-2 cells.

Degree: 2011, Iowa State University

URL: https://lib.dr.iastate.edu/etd/10126

► Public interest in botanical supplements has increased greatly in recent years as various plant materials might be used for anti-inflammatory, immunostimulatory, antioxidant and cancer preventive… (more)

▼ Public interest in botanical supplements has increased greatly in recent years as various plant materials might be used for anti-inflammatory, immunostimulatory, antioxidant and cancer preventive effects. Our long term goal is to improve our understanding of the characteristics of phytochemicals that contribute to human health benefits on gut functions, and thereby pave the way for optimizing herbal supplements for study in future clinical trials. In this dissertation, the overarching hypotheses were that major components of the ethanolic extracts of Echinacea, alkamides and ketones, and caffeic acid derivatives in the ethanolic extract of Prunella vulgaris, will be transferred by Caco-2 cell monolayers and caffeic acid derivatives will enhance the intestinal epithelial barrier function. Echinacea has long been used as phytotherapy for wound healing, pain relief and treatment of the common cold. In the first study, Bauer alkamides, the key components contained in Echinacea sanguinea and Echinaceapallida, transferred across the Caco-2 cell monolayer via passive diffusion, independent of other constituents in plant extract. The apparent permeability coefficients (Papp) were 2.8 y 1.5- 43.8 y 11.2 cm/s y10-6 for tested three alkamides and the order of the transfer of them across Caco-2 cells was increased paralleled with compound hydrophicility. Tested alkamides were seemingly N-glucuronidated and both Echinacea extracts stimulated apparent glucuronidation and basolateral efflux of alkamide metabolites. Bauer ketone 24 was totally metabolized to more hydrophilic metabolites as a pure compound, but not found in either Echinacea species. The addition of Bauer alkamides (175-230 yM) as well as the ethanolic extracts of E. sanguinea at 1 mg/mL (containing 85 yM of alkamide 8, 2 yM of alkamide 10, and 0.7 yM of alkamide 11) and E. pallida at 5 mg/mL (containing 215 yM of alkamide 8, 25 yM of alkamide 10, and 45 yM of alkamide 11) reduced the efflux of the P-glycoprotein transporter (P-gp) probe calcein-AM from Caco-2 cells. These results suggest that other constituents in the plant extract had a facilitating effect on the metabolism and efflux of alkamides and ketones from Echinacea, which would improve the therapeutic benefits of these extracts, and that alkamides and Echinacea extracts might be useful in potentiating some chemotherapeutics which are substrates for P-gp. Prunella vulgaris is a perennial herb known as self-heal used to treat sore throat, fever, and wounds. Rosmarinic acid is a caffeic acid derivative found in various botanicals, especially in P. vulgaris. Ursolic acid, a pentacyclic triterpene acid, is also found in P. vulgaris but especially concentrated in Salvia officinalis (sage), which has been traditionally used to treat inflammation in the oral cavity, and may also be of interest in inhibiting gastrointestinal inflammation which is relevant to colitis and colon cancer. In the second study, Papp for rosmarinic acid and rosmarinic acid in P. vulgaris extracts was 0.2 y 0.05 y 10-6 cm/s, significantly…

Subjects/Keywords: Bioavailability; Caco-2 cells; Caffeic acid; Echinacea; Metabolism; Prunella; Nutrition

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APA (6^th Edition):

Qiang, Z. (2011). Bioavailability and metabolism of botanical constituents and enhancement of intestinal barrier function by caffeic acid derivatives in Caco-2 cells. (Thesis). Iowa State University. Retrieved from https://lib.dr.iastate.edu/etd/10126

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Qiang, Zhiyi. “Bioavailability and metabolism of botanical constituents and enhancement of intestinal barrier function by caffeic acid derivatives in Caco-2 cells.” 2011. Thesis, Iowa State University. Accessed December 09, 2019. https://lib.dr.iastate.edu/etd/10126.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Qiang, Zhiyi. “Bioavailability and metabolism of botanical constituents and enhancement of intestinal barrier function by caffeic acid derivatives in Caco-2 cells.” 2011. Web. 09 Dec 2019.

Vancouver:

Qiang Z. Bioavailability and metabolism of botanical constituents and enhancement of intestinal barrier function by caffeic acid derivatives in Caco-2 cells. [Internet] [Thesis]. Iowa State University; 2011. [cited 2019 Dec 09]. Available from: https://lib.dr.iastate.edu/etd/10126.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Qiang Z. Bioavailability and metabolism of botanical constituents and enhancement of intestinal barrier function by caffeic acid derivatives in Caco-2 cells. [Thesis]. Iowa State University; 2011. Available from: https://lib.dr.iastate.edu/etd/10126

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Manitoba

4. Sabra, Ali. Physiological and Biochemical Responses of Three Echinacea Species to Salinity Stress.

Degree: Biological Sciences, 2012, University of Manitoba

URL: http://hdl.handle.net/1993/16681

► To determine the level of salt tolerance of the medicinal plant Echinacea, the physiological and biochemical characteristics of E. purpurea, E. pallida and E. angustifolia… (more)

▼ To determine the level of salt tolerance of the medicinal plant Echinacea, the physiological and biochemical characteristics of E. purpurea, E. pallida and E. angustifolia exposed to different NaCl levels (0, 50, 75, and 100 mM) were evaluated under hydroponic culture. Dry weights of shoots and roots were not affected by salinity; however E. purpurea and E. pallida exhibited higher survival rate than E. angustifolia, which also showed high salt injury index and electrolyte leakage compared to the other two species. Gas exchange (photosynthetic rate, stomatal conductance, and transpiration rate) showed a decline with increasing salt concentrations in all species with a more pronounced reduction in E. angustifolia. E. purpurea was able to retain more Na+ in the roots than the other two species showing its capacity to regulate Na+ translocation to shoots (Na+ exclusion). Moreover, the activities of two major antioxidant enzymes; superoxide dismutase (SOD) and ascorbate peroxidase (APX) were increased by salinity in E purperea, while the activities were decreased in E. angustifolia. The characteristic phytochemical profiles of caffeic acid derivatives (CADs) and alkamides/ketones were obtained for the three species, and quantitative changes were determined. Cichoric acid, the major CAD in E. purpurea, was increased with salinity up to 75 mM NaCl. A relative increase in alkamides and CADs was recorded in E. angustifolia, while in E. pallida, the level of echinacoside and major ketones (22 and 24) decreased, suggesting that the medicinal value of this species was compromised by salt stress. First evidence of salt-induced changes in alkamides and ketones in Echinacea was demonstrated in this study. Activity of phenylalanine ammonia-lyase (PAL), the major enzyme involved in the biosynthesis of CADs, was increased only in the roots of E. purpurea, further reflecting the differences in salt tolerance between species. It can be concluded that Echinacea species exhibited a limited degree of salt tolerance; however, E. purpurea showed a higher tolerance than E. pallida and E. angustifolia. This tolerance was mainly attributed to the increase in Na+ exclusion capacity, antioxidant activities and PAL activity. Advisors/Committee Members: Renault, Sylvie (Biological Sciences) (supervisor), Daayf, Fouad (Plant Science) Schroeder, Dana (Biological Sciences) Rajashekar C.B. (Horticulture, Forestry, and Recreation Resources, Kansas State University) (examiningcommittee).

Subjects/Keywords: Echinacea; Salinity; Caffeic acid derivatives; Alkamides; Growth; Antioxidant activities

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sabra, A. (2012). Physiological and Biochemical Responses of Three Echinacea Species to Salinity Stress. (Thesis). University of Manitoba. Retrieved from http://hdl.handle.net/1993/16681

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Sabra, Ali. “Physiological and Biochemical Responses of Three Echinacea Species to Salinity Stress.” 2012. Thesis, University of Manitoba. Accessed December 09, 2019. http://hdl.handle.net/1993/16681.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Sabra, Ali. “Physiological and Biochemical Responses of Three Echinacea Species to Salinity Stress.” 2012. Web. 09 Dec 2019.

Vancouver:

Sabra A. Physiological and Biochemical Responses of Three Echinacea Species to Salinity Stress. [Internet] [Thesis]. University of Manitoba; 2012. [cited 2019 Dec 09]. Available from: http://hdl.handle.net/1993/16681.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Sabra A. Physiological and Biochemical Responses of Three Echinacea Species to Salinity Stress. [Thesis]. University of Manitoba; 2012. Available from: http://hdl.handle.net/1993/16681

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Mississippi State University

5. Liao, Yu-chen. Identification of purpurogallin in brewed beverages and effect of roasting on antioxidant activity and phenolic compounds in coffees.

Degree: PhD, Food Science, Nutrition, and Health Promotion, 2015, Mississippi State University

URL: http://sun.library.msstate.edu/ETD-db/theses/available/etd-07272015-120432/ ;

► Coffee contains many antioxidants including purpurogallin, which is a hydrophobic phenolic antioxidant that is difficult to measure with reported methods. A method combining solid-phase… (more)

Subjects/Keywords: quinic acid; antioxidant; chlorogenic acid; tea; SPE; roasting; coffee; cocoa; brewed beverages; caffeic acid; shikimic acid; gallic acid; pyrogallol; purpurogallin

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Liao, Y. (2015). Identification of purpurogallin in brewed beverages and effect of roasting on antioxidant activity and phenolic compounds in coffees. (Doctoral Dissertation). Mississippi State University. Retrieved from http://sun.library.msstate.edu/ETD-db/theses/available/etd-07272015-120432/ ;

Chicago Manual of Style (16^th Edition):

Liao, Yu-chen. “Identification of purpurogallin in brewed beverages and effect of roasting on antioxidant activity and phenolic compounds in coffees.” 2015. Doctoral Dissertation, Mississippi State University. Accessed December 09, 2019. http://sun.library.msstate.edu/ETD-db/theses/available/etd-07272015-120432/ ;.

MLA Handbook (7^th Edition):

Liao, Yu-chen. “Identification of purpurogallin in brewed beverages and effect of roasting on antioxidant activity and phenolic compounds in coffees.” 2015. Web. 09 Dec 2019.

Vancouver:

Liao Y. Identification of purpurogallin in brewed beverages and effect of roasting on antioxidant activity and phenolic compounds in coffees. [Internet] [Doctoral dissertation]. Mississippi State University; 2015. [cited 2019 Dec 09]. Available from: http://sun.library.msstate.edu/ETD-db/theses/available/etd-07272015-120432/ ;.

Council of Science Editors:

Liao Y. Identification of purpurogallin in brewed beverages and effect of roasting on antioxidant activity and phenolic compounds in coffees. [Doctoral Dissertation]. Mississippi State University; 2015. Available from: http://sun.library.msstate.edu/ETD-db/theses/available/etd-07272015-120432/ ;

Universidade Federal de Santa Maria

6. Javed Anwar. Os efeitos do ácido cafeico e do éster fenetil do ácido cafeico sobre as atividades da acetilcolinesterase e das ecto-nucleotidases em ratos.

Degree: 2013, Universidade Federal de Santa Maria

URL: http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=4839

► Os compostos fenólicos e seus derivados constituem uma importante família de compostos naturais. O ácido cafeico (AC) e o éster fenetil do ácido cafeico (CAPE)… (more)

▼ Os compostos fenólicos e seus derivados constituem uma importante família de compostos naturais. O ácido cafeico (AC) e o éster fenetil do ácido cafeico (CAPE) são membros importantes dessa família e compartilham algumas aplicações biológicas, tais como: antioxidante, neuroprotetor, antiinflamatório, antiproliferativo, antibacteriano, antiviral, antiaterosclerótico e anticancerígeno. Entretanto, a literatura relata algumas atividades pró-oxidantes, dependendo do ambiente celular. Devido a estas propriedades patofisiológicas, aumentou o interesse com o objetivo de avaliar o efeito de CA e CAPE sob as atividades das enzimas purinérgicas e da acetilcolinesterase (AChE), tanto no Sistema Nervoso Periférico (SNP) como no Sistema Nervoso Central (SNC). Previamente, nosso grupo de pesquisa relatou que o composto fenólico tem a capacidade de alterar as atividades dessas enzimas. A AChE rapidamente hidrolisa a acetilcolina (ACh) em tecidos neuronais e não neuronais, mediando algumas doenças neurodegenerativas. Ao lado da ACh, o ATP (como coneurotransmissor) e adenosina são importantes moléculas sinalizadoras, comunicando as células em ambos os SNP e do SNC. Nas vias de sinalização extracelulares, os nucleotídeos de adenina e seus derivados podem ser acoplados a receptores específicos e desse modo ter um papel crucial no sistema nervoso, sistema vascular e imune. Uma vez liberadas, estas moléculas são hidrolisadas por uma cascata de enzimas incluindo a ectonucleosídeo trifosfato difosfoidrolase (NTPDase; EC 3.6.1.5, CD39), 5 - nucleotidase (EC 3.1.3.5, CD73), ectonucleotideo pirofosfatase/fosfodiesterase (E-NPP), modulando definitivamente as vias de sinalização do funcionamento normal do sistema nervoso, sistema vascular e imune. Além disso, a adenosina deaminase (ADA) e a xantina oxidase (XO) degradam a adenosina e a xantina, respectivamente, as quais controlam o funcionamento de mecanismos em eventos celulares. As enzimas encontradas em tecidos neuronais e não neuronais como a AChE, a NTPDase, a 5 -nucleotidase, a E-NPP e a ADA regulam eventos celulares incluindo a neurotransmissão, inflamação e processos trombogênicos. Com essas informações, nós introduzimos a hipótese de avaliar primeiramente os efeitos in vitro de CA na atividade da AChE periférica e no sistema central colinérgico de ratos. Os resultados demonstraram que o CA modula significativamente o sistema colinérgico no estudo in vitro. Essa modulação demonstra aparentemente que o CA (estrutura fenólica) possui propriedades de ação que altera a neurotransmissão. Portanto, a hipótese de se avaliar os efeitos in vivo de CA na atividade da AChE, NTPDase, E-NPP, 5 -nucleotidase, ADA e da agregação de plaquetas em diferentes tecidos de ratos tornou-se evidente. Para esse estudo, os animais foram tratados durante 30 dias e sacrificados após o teste comportamental. Os resultados do experimento demonstraram que o CA aumentou significativamente a atividade da AChE em hipocampo, hipotálamo, ponte e nos linfócitos, enquanto que no córtex cerebral, cerebelo e estriado a AChE… Advisors/Committee Members: Maria Rosa Chitolina Schetinger, Félix Alexandre Antunes Soares, Margareth Linde Athayde, Daniela Bitencourt Rosa Leal, Elizandra Braganhol.

Subjects/Keywords: éster fenetil do ácido cafeico; ácido cafeico; purinoreceptores; ectonucleotidases; nucleotídeo de adenina; acetilcolina; acetilcolinesterase; CIENCIAS BIOLOGICAS; actylcholine; actylcholinesterase; adenin nucleotides; ecto-nucleotidases; purinoreceptors; caffeic acid; caffeic acid phenethyl ester

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Anwar, J. (2013). Os efeitos do ácido cafeico e do éster fenetil do ácido cafeico sobre as atividades da acetilcolinesterase e das ecto-nucleotidases em ratos. (Thesis). Universidade Federal de Santa Maria. Retrieved from http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=4839

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Anwar, Javed. “Os efeitos do ácido cafeico e do éster fenetil do ácido cafeico sobre as atividades da acetilcolinesterase e das ecto-nucleotidases em ratos.” 2013. Thesis, Universidade Federal de Santa Maria. Accessed December 09, 2019. http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=4839.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Anwar, Javed. “Os efeitos do ácido cafeico e do éster fenetil do ácido cafeico sobre as atividades da acetilcolinesterase e das ecto-nucleotidases em ratos.” 2013. Web. 09 Dec 2019.

Vancouver:

Anwar J. Os efeitos do ácido cafeico e do éster fenetil do ácido cafeico sobre as atividades da acetilcolinesterase e das ecto-nucleotidases em ratos. [Internet] [Thesis]. Universidade Federal de Santa Maria; 2013. [cited 2019 Dec 09]. Available from: http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=4839.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Anwar J. Os efeitos do ácido cafeico e do éster fenetil do ácido cafeico sobre as atividades da acetilcolinesterase e das ecto-nucleotidases em ratos. [Thesis]. Universidade Federal de Santa Maria; 2013. Available from: http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=4839

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

7. Baranauskaitė, Agnė. Kavos rūgšties fenetilo esterio poveikio inkstų mitochondrijoms tyrimas.

Degree: Master, Pharmacy, 2014, Lithuanian Academic Libraries Network (LABT)

URL: http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2014~D_20140618_215632-14448 ;

►

Tyrimo tikslas: ištirti kavos rūgšties fenetilo esterio (CAFE) poveikį išemijos paveiktų žiurkės inkstų mitochondrijų funkcijoms. Uždaviniai: įvertinti tiesioginį in vitro CAFE poveikį inkstų mitochondrijų funkcijoms;… (more)

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Tyrimo tikslas: ištirti kavos rūgšties fenetilo esterio (CAFE) poveikį išemijos paveiktų žiurkės inkstų mitochondrijų funkcijoms. Uždaviniai: įvertinti tiesioginį in vitro CAFE poveikį inkstų mitochondrijų funkcijoms; CAFE poveikį išemijos (20 min) in vitro paveiktų inkstų mitochondrijų funkcijoms; kvėpavimo grandinės I komplekso aktyvumui; mitochondrijų gebėjimui kaupti Ca2+. Metodai. Wistar veislės žiurkių patinėliai buvo skirstomi į 4 grupes: kontrolinė grupė, 20 min trukmės išemijos, CAFE 22 mg/kg ir CAFE 34 mg/kg. CAFE buvo leidžiamas 1,5 h prieš sukeliant išemiją. Mitochondrijos buvo išskiriamos diferencinio centrifugavimo būdu. Mitochondrijų kvėpavimo greitis mitochondrijoms oksiduojant I ir II komplekso substratus buvo registruojamas poliarografiškai Klarko tipo elektrodu. Ca2+ sugėrimas buvo matuojamas fluorimetriškai. Mitochondrijų kvėpavimo grandinės I komplekso aktyvumas buvo tiriamas spektrofotometriškai. Rezultatai: CAFE (0,7 – 4,5 M) didina mitochondrijų kvėpavimo greitį 2-oje metabolinėje būsenoje nuo 15 % iki 34 % ir neveikė mitochondrijų kvėpavimo greičio 3-ioje metabolinėje būsenoje (VADP). Didesnės koncentracijos (5,2 - 6,5 µM) slopina mitochondrijų kvėpavimo greitį VADP (16 % ir 43 % atitinkamai). Tiriant CAFE poveikį 20 min išemijos in vitro paveiktų mitochondrijų funkcijoms, nustatyta, jog 22 mg/kg ir 34 mg/kg CAFE, intraperitonealinė injekcija 1,5 h prieš sukeliant inkstų išemiją, 20 % (p<0,05) pagerino mitochondrijų kvėpavimo greitį VADP bei... [toliau žr. visą tekstą]

The aim of investigation: to analyse the effect of caffeic acid phenethyl ester (CAPE) on kidney mitochodrial functions. Objectives: to evaluate direct in vitro effect of CAPE on kidney mitochondrial functions; the impact of CAPE on ischemia (20 min) in vitro affected kidney mitochondrial functions; on the mitochondrial respiratory chain complex I activity and on the mitochondria capability to accumulate Ca2+. Methods. Wistar rats were pretreated intraperitoneal with 22 mg/kg and 34 mg/kg of CAPE. Animals were divided into 4 groups: control group, 20 min of ischemia, CAPE 22 mg/kg group and CAPE 34 mg/kg group. Mitochondria were isolated by means of differential centrifugation. The mitochondrial respiration rate while oxidizing complex I and II dependent substrates was registered polarographically with Clark-type electrode. Ca2+ accumulation was measured fluorometrically. Activity of complex I was measured spectrophotometrically. Results: the results shown that CAPE 0,7 - 4,5 µM increases mitochondrial State 2 respiration rate by 15 - 34 % and has no effect on the State 3 respiration rate. Higher concentrations (5,2 - 6,5 µM) decreased mitochondrial State 3 respiration rate by 16% and 43%, respectively. Pretreatment with CAPE (22 mg/kg and 34 mg/kg) increased (by 20%) the ischemia suppressed mitochondrial State 3 respiration rate and respiratory control index. CAPE had no effect on succinate oxydation. Pretreatment with CAPE (22 mg/kg and 34 mg/kg) increased Ca2+ accumulation... [to full text]

Advisors/Committee Members: Trumbeckaitė, Sonata (Master’s thesis supervisor), Banienė, Rasa (Master’s thesis advisor), Grincevičius, Jonas (Master’s thesis reviewer), Savickienė, Nijolė (Master’s degree committee chair), Dagilytė, Audronė (Master’s degree session secretary), Janulis, Valdimaras (Master’s degree committee member), Ivanauskas, Liudas (Master’s degree committee member), Savickas, Arūnas (Master’s degree committee member), Briedis, Vitalis (Master’s degree committee member), Ramanauskienė, Kristina (Master’s degree committee member), Inkėninė, Asta Marija (Master’s degree committee member), Jakštas, Valdas (Master’s degree committee member), Drakšienė, Gailutė (Master’s degree committee member), Radžiūnas, Raimundas (Master’s degree committee member), Skyrius, Vaidas (Master’s degree committee member), Brusokas, Valdemaras (Master’s degree committee member), Barsteigienė, Zita (Master’s degree committee member), Marksienė, Rūta (Master’s degree committee member), Ževžikovas, Andrejus (Master’s degree committee member), Vitkevičius, Konradas (Master’s degree committee member), Gumbrevičius, Gintautas (Master’s degree committee member), Ragažinskienė, Ona (Master’s degree committee member), Maruška, Audrius (Master’s degree committee member), Martinėnas, Žydrūnas (Master’s degree committee member), Kuncaitė, Giedrė (Master’s degree committee member), Zulanienė, Audronė (Master’s degree committee member), Balanaškienė, Rima (Master’s degree committee member), Marcinkevičienė, Rasa (Master’s degree committee member), Jakubauskas, Mindaugas (Master’s degree committee member), Budrikienė, Aušra (Master’s degree committee member), Švarcaitė, Jūratė (Master’s degree committee member).

Subjects/Keywords: Kavos rūgšties fenetilo esteris; Inkstų išemija; Mitochondrijos; Caffeic acid phenethyl ester; Kidney ischemia; Mitochondria

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Baranauskaitė, Agnė. (2014). Kavos rūgšties fenetilo esterio poveikio inkstų mitochondrijoms tyrimas. (Masters Thesis). Lithuanian Academic Libraries Network (LABT). Retrieved from http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2014~D_20140618_215632-14448 ;

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Author name may be incomplete

Chicago Manual of Style (16^th Edition):

Baranauskaitė, Agnė. “Kavos rūgšties fenetilo esterio poveikio inkstų mitochondrijoms tyrimas.” 2014. Masters Thesis, Lithuanian Academic Libraries Network (LABT). Accessed December 09, 2019. http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2014~D_20140618_215632-14448 ;.

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Author name may be incomplete

MLA Handbook (7^th Edition):

Baranauskaitė, Agnė. “Kavos rūgšties fenetilo esterio poveikio inkstų mitochondrijoms tyrimas.” 2014. Web. 09 Dec 2019.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Vancouver:

Baranauskaitė, Agnė. Kavos rūgšties fenetilo esterio poveikio inkstų mitochondrijoms tyrimas. [Internet] [Masters thesis]. Lithuanian Academic Libraries Network (LABT); 2014. [cited 2019 Dec 09]. Available from: http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2014~D_20140618_215632-14448 ;.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Council of Science Editors:

Baranauskaitė, Agnė. Kavos rūgšties fenetilo esterio poveikio inkstų mitochondrijoms tyrimas. [Masters Thesis]. Lithuanian Academic Libraries Network (LABT); 2014. Available from: http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2014~D_20140618_215632-14448 ;

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Washington State University

8. [No author]. A STRUCTURE-BASED ANALYSIS OF BIOENGINEERING TARGETS IN PHENYL METABOLISM FOR GREEN BIOTECHNOLOGY APPLICATIONS .

Degree: 2014, Washington State University

URL: http://hdl.handle.net/2376/5426

► Green biotechnology is the engineering of biological systems for use in environmental and agricultural purposes. Two major areas of environmental biotechnology are bioremediation and biofuel… (more)

▼ Green biotechnology is the engineering of biological systems for use in environmental and agricultural purposes. Two major areas of environmental biotechnology are bioremediation and biofuel production. Enzyme engineering is often the first approach employed for these objectives because enzymes are the functional units of pathways. Deciphering both the structure and structure-function relationship of enzymes provides a foundation of knowledge to boost rational enzyme engineering efforts. Bioremediation is the clean-up of industrial wastes with enzymes or organisms. The key to developing these technologies lies in understanding xenobiotic metabolism: the natural breakdown of foreign substances. An important facet of xenobiotic metabolism is glutathione cycling. In prokaryotes and lower eukaryotes, the highly conserved glutathionyl-hydroquinone reductases (GS-HQRs) are essential for this cycling. The structures of GS-HQRs are dimeric and consist of a thioredoxin-like N-terminal domain that binds glutathione and an helical C-terminal domain that contains a non-specific hydrophobic binding site and a tyrosine network capable of facile proton abstraction and donation. A nucleophilic cysteine stabilized in thiolate form at pH above 7.2, along with the tyrosine network, catalyzes the reductive removal of the glutathione from glutathionyl-hydroquinone conjugates. All essential active site residues are highly conserved in the GS-HQRs from several kingdoms of life. Bioethanol is the biofuel equivalent of gasoline. A key roadblock to the economical production of bioethanol from agricultural residue is lignin. Lignin is an essential biopolymer for the survival of terrestrial plants but inhibits the release of fermentable sugar polymers from lignocellulosic biomass. Both reduction of lignin content and modification of lignin composition, namely the S:G ratio, in monocots increases sugar yields. A crucial enzyme in the conversion of S to G units is caffeic acid O-methyltransferase (COMT). The structure and kinetic mechanism of Sorghum bicolor's COMT were determined. SbCOMT catalyzes the S-adenosylmethionine-dependent O-methylation of both the 3'- and 5'-hydroxyl of phenylpropanoids. A rapid equilibrium random mechanism for caffeic acid and partial substrate inhibition for 5-hydroxyconiferaldehyde is observed. SbCOMT's structure is highly-conserved among two monocots and one dicot. Additionally, one missense mutation of SbCOMT associated with the favorable brown midrib phenotype was determined to reduce the amount of secondary structure.

Subjects/Keywords: Biochemistry; caffeic acid O-methyltransferase; glutathionyl-hydroquinone reductases; green biotechnology; lignin; sorghum bicolor; xenobiotic metabolism

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

author], [. (2014). A STRUCTURE-BASED ANALYSIS OF BIOENGINEERING TARGETS IN PHENYL METABOLISM FOR GREEN BIOTECHNOLOGY APPLICATIONS . (Thesis). Washington State University. Retrieved from http://hdl.handle.net/2376/5426

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

author], [No. “A STRUCTURE-BASED ANALYSIS OF BIOENGINEERING TARGETS IN PHENYL METABOLISM FOR GREEN BIOTECHNOLOGY APPLICATIONS .” 2014. Thesis, Washington State University. Accessed December 09, 2019. http://hdl.handle.net/2376/5426.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

author], [No. “A STRUCTURE-BASED ANALYSIS OF BIOENGINEERING TARGETS IN PHENYL METABOLISM FOR GREEN BIOTECHNOLOGY APPLICATIONS .” 2014. Web. 09 Dec 2019.

Vancouver:

author] [. A STRUCTURE-BASED ANALYSIS OF BIOENGINEERING TARGETS IN PHENYL METABOLISM FOR GREEN BIOTECHNOLOGY APPLICATIONS . [Internet] [Thesis]. Washington State University; 2014. [cited 2019 Dec 09]. Available from: http://hdl.handle.net/2376/5426.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

author] [. A STRUCTURE-BASED ANALYSIS OF BIOENGINEERING TARGETS IN PHENYL METABOLISM FOR GREEN BIOTECHNOLOGY APPLICATIONS . [Thesis]. Washington State University; 2014. Available from: http://hdl.handle.net/2376/5426

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

9. Celso de Oliveira Rezende Júnior. SÍNTESE DE CANDIDATOS A NOVOS INIBIDORES DA ENZIMA HIV-INTEGRASE.

Degree: 2010, Universidade Federal de Juiz de Fora

URL: http://www.bdtd.ufjf.br/tde_busca/arquivo.php?codArquivo=849

► Este trabalho trata da síntese de cicloexanopoliois derivados do ácido quínico, esterificados com os ácidos cafeico e gálico. Esses compostos são candidatos a novos agentes… (more)

Subjects/Keywords: QUIMICA; HIV-integrase; Ácidos cafeoíl-quínicos; Cicloexanopoliois; Ácido cafeico; Ácido gálico; HIV-integrase; Caffeoyl quinic acid; Ciclohexanepoliols; Caffeic acid; Gallic acid

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APA (6^th Edition):

Júnior, C. d. O. R. (2010). SÍNTESE DE CANDIDATOS A NOVOS INIBIDORES DA ENZIMA HIV-INTEGRASE. (Thesis). Universidade Federal de Juiz de Fora. Retrieved from http://www.bdtd.ufjf.br/tde_busca/arquivo.php?codArquivo=849

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Júnior, Celso de Oliveira Rezende. “SÍNTESE DE CANDIDATOS A NOVOS INIBIDORES DA ENZIMA HIV-INTEGRASE.” 2010. Thesis, Universidade Federal de Juiz de Fora. Accessed December 09, 2019. http://www.bdtd.ufjf.br/tde_busca/arquivo.php?codArquivo=849.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Júnior, Celso de Oliveira Rezende. “SÍNTESE DE CANDIDATOS A NOVOS INIBIDORES DA ENZIMA HIV-INTEGRASE.” 2010. Web. 09 Dec 2019.

Vancouver:

Júnior CdOR. SÍNTESE DE CANDIDATOS A NOVOS INIBIDORES DA ENZIMA HIV-INTEGRASE. [Internet] [Thesis]. Universidade Federal de Juiz de Fora; 2010. [cited 2019 Dec 09]. Available from: http://www.bdtd.ufjf.br/tde_busca/arquivo.php?codArquivo=849.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Júnior CdOR. SÍNTESE DE CANDIDATOS A NOVOS INIBIDORES DA ENZIMA HIV-INTEGRASE. [Thesis]. Universidade Federal de Juiz de Fora; 2010. Available from: http://www.bdtd.ufjf.br/tde_busca/arquivo.php?codArquivo=849

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

10. Andreadelis, Ioannis. Χρήση μοριακής δυναμικής σε συνδυασμό με φασματοσκοπία NMR για την πρόταση σύνθεσης βιοδραστικών μορίων και συστημάτων μεταφοράς τους.

Degree: 2019, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ)

URL: http://hdl.handle.net/10442/hedi/46437

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In this thesis, the role of complexing natural and pharmaceutical molecules with proven beneficial health properties with 2-hydroxylpropyl-β-cyclodextrin (HP-β-CD) was studied. In the first part… (more)

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In this thesis, the role of complexing natural and pharmaceutical molecules with proven beneficial health properties with 2-hydroxylpropyl-β-cyclodextrin (HP-β-CD) was studied. In the first part of the thesis, the molecular interactions between caffeic and rosmarinic acid in hydroxypropyl-β-cyclodextrin were studied using a combination of NMR spectroscopy and molecular modeling. Changes in the chemical shifts of carbon (13C CP/MAS) and hydrogen spectroscopy as well as intermolecular within the complex NOEs have clarified the orientation and conformations of the natural products into the cyclodextrin. Molecular dynamics and Isothermal Calorimetry (ITC) experiments were also conducted to investigate the stability and degree of substitution of the cyclodextrin. In the second part of the thesis, complexes of candesartan and candesartan cilexetil with HP-β-CD were characterized using ESI QTQF HRMS and solid state NMR and in particular CP/MAS spectroscopy. The 13C CP/MAS results showed broad peaks especially in the aromatic region, thus confirming the strong interactions between cyclodextrin and drugs. This experimental evidence was in accordance with MD simulations and quantum mechanical calculations. The synthesized and characterized complexes were evaluated biologically in vitro. It was shown that as a result of CAN’s complexation, CAN exerts higher antagonistic activity than CC. This intriguing result agrees with binding free energy calculations, which predicted efficient binding with CC while CAN binding was not favored. Thus, CC cannot be released easily from HP-β-CD and as a result is not suggested as a new pharmaceutical formulation. The opposite holds truth for CAN complex which could be used as a novel antihypertensive pharmaceutical formulation.

Στην παρούσα διατριβή μελετήθηκε ο ρόλος της συμπλοκοποίησης φυσικών και φαρμακευτικών μορίων με αποδεδειγμένη ευεργετική δράση στην υγεία με τη 2-υδροξυπροπυλο-β-κυκλοδεξτρίνη (HP-β-CD ή 2HP-β-CD). Τα σύμπλοκα όλων των ενώσεων ταυτοποιήθηκαν με χρήση φασματομετρίας ESI QTOF HRMS. Στο πρώτο τμήμα της διδακτορικής διατριβής, περιγράφονται οι υπολογισμοί μοριακών αλληλεπιδράσεων κατά τη σύμπλεξη μεταξύ των φυσικών προϊόντων καφεϊκού και του ροσμαρινικού οξέος εντός της HP-β-CD χρησιμοποιώντας συνδυασμό φασματοσκοπίας NMR υγρής και στερεής κατάστασης (ssNMR) και μοριακής μοντελοποίησης. Οι μεταβολές των χημικών μετατοπίσεων ανιχνεύθησαν κατά τη σύμπλεξη στα φάσματα άνθρακα περιστροφής υπό μαγική γωνία και χρήσης διασταυρούμενης πόλωσης 13C CP/MAS καθώς και πρωτονίων στα υψηλής διακριτικής ικανότητας σε διάλυμα φασμάτων 1Η NMR. Τα αποτελέσματα των πειραμάτων αυτών συμπληρούμενα και με αυτά των δύο διαστάσεων 2D NOESY, αποσαφήνισαν τον προσανατολισμό και τις διαμορφώσεις που λαμβάνουν τα φυσικά προϊόντα εντός της υπό μελέτη κυκλοδεξτρίνης. Διεξήχθηκαν επίσης πειράματα μοριακής δυναμικής και θερμιδομετρίας ισοθερμικής τιτλοδότησης (ITC) για τη διερεύνηση μέσω θερμοδυναμικών παραμέτρων της σταθερότητας των δημιουργούμενων συμπλόκων και του βαθμού υποκατάστασης της…

Subjects/Keywords: Ροσμαρινικό οξύ; Καφεϊκό οξύ; Καντεσαρτάνη; Σιλεξετίλη της καντεσαρτάνης; Μοριακή δυναμική; Rosmarinic acid; Caffeic acid; Candesartan; Candesartan cilexetil; Molecular dynamics

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Andreadelis, I. (2019). Χρήση μοριακής δυναμικής σε συνδυασμό με φασματοσκοπία NMR για την πρόταση σύνθεσης βιοδραστικών μορίων και συστημάτων μεταφοράς τους. (Thesis). National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Retrieved from http://hdl.handle.net/10442/hedi/46437

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Andreadelis, Ioannis. “Χρήση μοριακής δυναμικής σε συνδυασμό με φασματοσκοπία NMR για την πρόταση σύνθεσης βιοδραστικών μορίων και συστημάτων μεταφοράς τους.” 2019. Thesis, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Accessed December 09, 2019. http://hdl.handle.net/10442/hedi/46437.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Andreadelis, Ioannis. “Χρήση μοριακής δυναμικής σε συνδυασμό με φασματοσκοπία NMR για την πρόταση σύνθεσης βιοδραστικών μορίων και συστημάτων μεταφοράς τους.” 2019. Web. 09 Dec 2019.

Vancouver:

Andreadelis I. Χρήση μοριακής δυναμικής σε συνδυασμό με φασματοσκοπία NMR για την πρόταση σύνθεσης βιοδραστικών μορίων και συστημάτων μεταφοράς τους. [Internet] [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2019. [cited 2019 Dec 09]. Available from: http://hdl.handle.net/10442/hedi/46437.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Andreadelis I. Χρήση μοριακής δυναμικής σε συνδυασμό με φασματοσκοπία NMR για την πρόταση σύνθεσης βιοδραστικών μορίων και συστημάτων μεταφοράς τους. [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2019. Available from: http://hdl.handle.net/10442/hedi/46437

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

11. Barbosa, André Luiz. Cultura de tecidos e regeneração de plantas transgênicas a partir de calos embriogênicos e de folhas imaturas de cana-de-açúcar.

Degree: Mestrado, Fisiologia e Bioquímica de Plantas, 2010, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/11/11144/tde-30062010-080258/ ;

► A cana-de-açúcar é uma monocotiledônea poliplóide, alógama que possui baixa taxa reprodutiva devido a dificuldade de florescimento. Devido estas características genéticas e fisiológicas os programas… (more)

▼ A cana-de-açúcar é uma monocotiledônea poliplóide, alógama que possui baixa taxa reprodutiva devido a dificuldade de florescimento. Devido estas características genéticas e fisiológicas os programas de melhoramento são longos e laboriosos. Alternativamente, modernas aplicações da biotecnologia visam contribuir com o desenvolvimento de novos cultivares. Neste trabalho estudou-se a metodologia de cultura de tecidos a partir de discos de folhas imaturas para o estabelecimento da cultura de calos embriogênicos e regeneração de plantas a partir dos calos embriogênicos e diretamente, a partir de folhas imaturas. O objetivo principal foi contribuir para o desenvolvimento de métodos eficientes para produção de plantas transgênicas a partir de calos e folhas imaturas, considerando-se a crescente necessidade de produção de novos cultivares com características agronômicas específicas. Diversas concentrações de 2,4-D e cinetina em meio MS foram testadas para o estabelecimento de calos altamente embriogênicos e para a indução da desdiferenciação celular nos discos foliares antecedendo a regeneração de plantas. Meios de cultura sem reguladores de crescimento (MS) e com a adição de BAP e ANA foram testados para a regeneração de plantas a partir de discos foliares. Calos embriogênicos com 12 a 20 semanas de cultivo produziram em média 3 a 5 plantas, em meio de regeneração MS. Folhas imaturas apresentaram elevado potencial de regeneração de plantas quando se utilizou 2,4-D em concentrações de 5 e 8 mg/L nos períodos de 5 e 8 dias no escuro. Houve indução a formação de embriões somáticos que resultaram em média 12 a 16 plantas por explante no período total de 7 a 10 semanas. Além disso, foi testado o pré-tratamento dos discos foliares em meio MS3K, contendo 2,4-D (3mg/L) e cinetina (0,1 mg/L), antes da transferência do discos para meio de regeneração MS. Os discos submetidos a este pré-tratamento durante 14, 21 e 28 dias apresentaram aumento significativo na eficiência de regeneração de plantas, variando em média de 41 a 50 plantas por disco foliar nas variedades RB835089 e RB855156. A redução no tempo para obtenção de plantas aliado ao aumento na média de plantas obtidas é a base para aumentar a eficiência de transformação genética de plantas. Experimentos de cotransformação dos genes neo e comt(AS), foram realizados por biolística. Em plantas regeneradas a partir de folhas imaturas da variedade RB835486, as análises de PCR confirmaram a incorporação do gene marcador neo em 57 e 90% das plantas em meio seletivo com geneticina (30 mg/L), sendo que a maior eficiência de regeneração de transgênicos (90%) foi obtida no pré-tratamento com o meio MS3K. Das plantas transgênicas para o gene neo, 7 e 38% também foram confirmadas para a incorporação do gene comt(AS). Nas plantas regeneradas a partir de calos embriogênicos em meio seletivo, as análises de PCR detectaram somente a incorporação do gene neo, o que ocorreu em 52% das plantas analisadas. Os resultados obtidos mostram que a cultura de discos de folhas imaturas para o processo de… Advisors/Committee Members: Carrer, Helaine.

Subjects/Keywords: Auxin; Biolística; Biolistics.; Caffeic acid-O-methyltransferase; Cana-de-açúcar; COMT; Cotransformation; Cytokinin; Genes; Growth regulators; Polimorfismo; Reguladores de crescimento vegetal.

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Barbosa, A. L. (2010). Cultura de tecidos e regeneração de plantas transgênicas a partir de calos embriogênicos e de folhas imaturas de cana-de-açúcar. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/11/11144/tde-30062010-080258/ ;

Chicago Manual of Style (16^th Edition):

Barbosa, André Luiz. “Cultura de tecidos e regeneração de plantas transgênicas a partir de calos embriogênicos e de folhas imaturas de cana-de-açúcar.” 2010. Masters Thesis, University of São Paulo. Accessed December 09, 2019. http://www.teses.usp.br/teses/disponiveis/11/11144/tde-30062010-080258/ ;.

MLA Handbook (7^th Edition):

Barbosa, André Luiz. “Cultura de tecidos e regeneração de plantas transgênicas a partir de calos embriogênicos e de folhas imaturas de cana-de-açúcar.” 2010. Web. 09 Dec 2019.

Vancouver:

Barbosa AL. Cultura de tecidos e regeneração de plantas transgênicas a partir de calos embriogênicos e de folhas imaturas de cana-de-açúcar. [Internet] [Masters thesis]. University of São Paulo; 2010. [cited 2019 Dec 09]. Available from: http://www.teses.usp.br/teses/disponiveis/11/11144/tde-30062010-080258/ ;.

Council of Science Editors:

Barbosa AL. Cultura de tecidos e regeneração de plantas transgênicas a partir de calos embriogênicos e de folhas imaturas de cana-de-açúcar. [Masters Thesis]. University of São Paulo; 2010. Available from: http://www.teses.usp.br/teses/disponiveis/11/11144/tde-30062010-080258/ ;

University of the Western Cape

12. Klein, Ashwil Johan. Modulation of soybean and maize antioxidant activities by Caffeic acid and nitric oxide under salt stress .

Degree: 2012, University of the Western Cape

URL: http://hdl.handle.net/11394/4057

► This study explores the roles of exogenously applied nitric oxide, exogenously applied caffeic acid and salt stress on the ontioxidant system in cereal (exemplified by… (more)

▼ This study explores the roles of exogenously applied nitric oxide, exogenously applied caffeic acid and salt stress on the ontioxidant system in cereal (exemplified by maize) and legume (using soybean as an example) plants together with their influence on membrane integrity and cell death. This study investigates changes in H₂O₂ content, root lipid peroxidation, root cell death and antioxidant enzymatic activity in maize roots in response to exogenously applied nitric oxide (NO) and salt stress. This part of the study is based on the partially understood interaction between NO and reactive oxygen species (ROS) such as H₂O₂ and the role of antioxidant enzymes in plant salt stress responses. The results show that application of salt (NaCl) results in elevated levels of H₂O₂ and an increase in lipid peroxidation, consequently leading to increased cell death. The study also shows that by regulating the production and detoxification of ROS through modulation of antioxidant enzymatic activities, NO plays a pivotal role in maize responses to salt stress. The study argues for NO as a regulator of redox homeostasis that prevents excessive ROS accumulation during exposure of maize to salinity stress that would otherwise be deleterious to maize. This study extends the role of exogenously applied NO to improve salt stress tolerance in cereals crops (maize) further to its role in enhancing salt stress tolerance in legumes. The effect of long-term exposure of soybean to NO and salt stress on root nodule antioxidant activity was investigated to demonstrate the role of NO in salt stress tolerance. The results show that ROS scavenging antioxidative enzymes like SOD, GPX and GR are differentially regulated in response to exogenous application of NO and salt stress. It remains to be determined if the NO induced changes in antioxidant enzyme activity under salt stress are sufficient to efficiently reduce ROS accumulation in soybean root nodules to levels close to those of unstressed soybean root nodules. Furthermore, this study investigates the effect of long-term exposure of soybean to exogenous caffeic acid (CA) and salt stress, on the basis of the established role of CA as an antioxidant and the involvement of antioxidant enzymes in plant salt stress responses. The effect of CA on soybean nodule number, biomass (determined on the basis of nodule dry weight, root dry weight and shoot dry weight), nodule NO content, and nodule cyclic guanosine monophosphate (cGMP) content in response to salt stress was investigated. Additionally, CA-induced changes in nodule ROS content, cell viability, lipid peroxidation and antioxidant enzyme activity as well as some genes that encode antioxidant enzymes were investigated in the presence or absence of salt stress. The study shows that long-term exposure of soybean to salt stress results in reduced biomass associated with accumulation of ROS, elevated levels of lipid peroxidation and elevated levels of cell death. However, exogenously applied CA reversed the negative effects of salt stress on… Advisors/Committee Members: Ludidi, Ndomelele Ndiko (advisor), Keyster, Marshall (advisor).

Subjects/Keywords: Nitric oxide; Salinity; Reactive oxygen species; Cell death; Antioxidant enzymes; Caffeic acid; Lipid peroxidation; Superoxide; Antioxidant gene expression; Salt stress tolerance

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APA (6^th Edition):

Klein, A. J. (2012). Modulation of soybean and maize antioxidant activities by Caffeic acid and nitric oxide under salt stress . (Thesis). University of the Western Cape. Retrieved from http://hdl.handle.net/11394/4057

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Klein, Ashwil Johan. “Modulation of soybean and maize antioxidant activities by Caffeic acid and nitric oxide under salt stress .” 2012. Thesis, University of the Western Cape. Accessed December 09, 2019. http://hdl.handle.net/11394/4057.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Klein, Ashwil Johan. “Modulation of soybean and maize antioxidant activities by Caffeic acid and nitric oxide under salt stress .” 2012. Web. 09 Dec 2019.

Vancouver:

Klein AJ. Modulation of soybean and maize antioxidant activities by Caffeic acid and nitric oxide under salt stress . [Internet] [Thesis]. University of the Western Cape; 2012. [cited 2019 Dec 09]. Available from: http://hdl.handle.net/11394/4057.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Klein AJ. Modulation of soybean and maize antioxidant activities by Caffeic acid and nitric oxide under salt stress . [Thesis]. University of the Western Cape; 2012. Available from: http://hdl.handle.net/11394/4057

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Kentucky

13. Zaffarano, Jennifer I. MINIMUM INHIBITORY CONCENTRATIONS OF TWO COMMON FOOD PHENOLIC COMPOUNDS AND THEIR EFFECT ON THE MICROBIAL ECOLOGY OF SWINE FECES IN VITRO.

Degree: 2003, University of Kentucky

URL: http://uknowledge.uky.edu/gradschool_theses/182

► Feeding sub-therapeutic levels of antibiotics to livestock has been associated withdevelopment and spread of antibiotic resistant bacteria. The present experiment was conductedto investigate the effect… (more)

Subjects/Keywords: Food phenolics; caffeic acid; chlorogenic acid; in vitro; microbial ecology

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APA (6^th Edition):

Zaffarano, J. I. (2003). MINIMUM INHIBITORY CONCENTRATIONS OF TWO COMMON FOOD PHENOLIC COMPOUNDS AND THEIR EFFECT ON THE MICROBIAL ECOLOGY OF SWINE FECES IN VITRO. (Masters Thesis). University of Kentucky. Retrieved from http://uknowledge.uky.edu/gradschool_theses/182

Chicago Manual of Style (16^th Edition):

Zaffarano, Jennifer I. “MINIMUM INHIBITORY CONCENTRATIONS OF TWO COMMON FOOD PHENOLIC COMPOUNDS AND THEIR EFFECT ON THE MICROBIAL ECOLOGY OF SWINE FECES IN VITRO.” 2003. Masters Thesis, University of Kentucky. Accessed December 09, 2019. http://uknowledge.uky.edu/gradschool_theses/182.

MLA Handbook (7^th Edition):

Zaffarano, Jennifer I. “MINIMUM INHIBITORY CONCENTRATIONS OF TWO COMMON FOOD PHENOLIC COMPOUNDS AND THEIR EFFECT ON THE MICROBIAL ECOLOGY OF SWINE FECES IN VITRO.” 2003. Web. 09 Dec 2019.

Vancouver:

Zaffarano JI. MINIMUM INHIBITORY CONCENTRATIONS OF TWO COMMON FOOD PHENOLIC COMPOUNDS AND THEIR EFFECT ON THE MICROBIAL ECOLOGY OF SWINE FECES IN VITRO. [Internet] [Masters thesis]. University of Kentucky; 2003. [cited 2019 Dec 09]. Available from: http://uknowledge.uky.edu/gradschool_theses/182.

Council of Science Editors:

Zaffarano JI. MINIMUM INHIBITORY CONCENTRATIONS OF TWO COMMON FOOD PHENOLIC COMPOUNDS AND THEIR EFFECT ON THE MICROBIAL ECOLOGY OF SWINE FECES IN VITRO. [Masters Thesis]. University of Kentucky; 2003. Available from: http://uknowledge.uky.edu/gradschool_theses/182

Universidade de Brasília

14. Thiago Cardoso Genaro de Mattos. Mecanismos da ação antioxidante dos ácidos caféico e tânico em sistemas contendo íons ferro.

Degree: 2009, Universidade de Brasília

URL: http://bdtd.bce.unb.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=5359

►

The present work was divided in three distinct chapters. In the first chapter, we performed a reevaluation of the 2-deoxyribose (2-DR) degradation assay for metal-mediated… (more)

▼

The present work was divided in three distinct chapters. In the first chapter, we performed a reevaluation of the 2-deoxyribose (2-DR) degradation assay for metal-mediated free radical formation.This assay is widely employed to evaluate the pro/antioxidant activity of pure compounds or plant extracts. However, we observed that Fe(III) a product of Fenton and Fe(II) autoxidation reactions reacts with 2-DR generating greats amounts of malondialdehyde (MDA). The results show that the reaction between Fe(III) and 2-DR is not influenced by buffer composition, by the addition of antioxidant compounds or iron chelators. This reaction consists in a methodological artifact that leads to antioxidant capacity underestimation or/and incorrect interpretation of the studied mechanisms. To correct this interference, we proposed a new assays blank based on the use of Fe(III). In chapters 2 and 3, we studied the effects of caffeic acid (CA) on Fenton and Fe(II) autoxidation reactions, respectively. Results were compared to tannic acid (TA), a polyphenol largely studied in our lab with known antioxidant activity. In both Fenton and Fe(II) autoxidation reactions, CA acted as antioxidant by inhibiting the iron-mediated 2-DR degradation, DMPO hydroxylation (only in the presence of Fenton reactants) and rat liver lipid peroxidation. The protection was dose-depedent on CA concentration and inversely correlated to iron concentration. CA performed its antioxidant activity in the micromolar range, which suggests a chelating antioxidant mechanism. Furthermore, we suggested a 1:2 Fe(II)-CA ratio that could be responsible for the inhibition of hydroxyl radical mediated oxidations. CA also presented a free radical scavenger activity by reducing ABTS radical and extending the lag phase of lipid peroxidation. Moreover, we observed that both CA and TA increased the rate of Fe(II) autoxidation reaction (by accelerating the oxygen consumption) and were able to reduce Fe(III) to Fe(II) by an inner-sphere electron transfer.

Este trabalho foi dividido em três capítulos distintos. No primeiro capítulo, foi feita uma reavaliação da metodologia da degradação oxidativa da 2-desoxirribose (2-DR) em sistemas geradores de radicais livres mediados por íons ferrosos. Este ensaio é amplamente utilizado para avaliar a atividade pró/antioxidante de moléculas isoladas ou extratos vegetais. Entretanto, verificou-se que o Fe(III) produto das reações de Fenton e autoxidação do Fe(II) reage com a 2-DR levando à formação de malonildialdeído (MDA). Os resultados mostram que a reação entre o Fe(III) e a 2-DR não é influenciada pela presença de antioxidantes, pelo tipo de tampão utilizado ou pela presença de quelantes de ferro. A reação do Fe(III) com a 2-DR consiste em um artefato metodológico, que não descontado propriamente (por meio de um novo branco proposto neste capítulo), pode levar à subestimação da atividade antioxidante ou a incorreta interpretação dos mecanismos de ação dos compostos estudados. Nos capítulos 2 e 3, foram estudados os efeitos do ácido caféico…

Advisors/Committee Members: Marcelo Hermes Lima, Francisco Ricardo da Cunha, Claudia Jorge do Nascimento, Alexandre Gustavo Soares do Prado.

Subjects/Keywords: Fenton; antioxidante; radicais livres; ácido caféico; ferro; ácido tânico; 2-desoxirribose; autoxidação; QUIMICA; iron; caffeic acid; tannic acid; antioxidant; free radicals; Fenton; autoxidation; 2-deoxyribose

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mattos, T. C. G. d. (2009). Mecanismos da ação antioxidante dos ácidos caféico e tânico em sistemas contendo íons ferro. (Thesis). Universidade de Brasília. Retrieved from http://bdtd.bce.unb.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=5359

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mattos, Thiago Cardoso Genaro de. “Mecanismos da ação antioxidante dos ácidos caféico e tânico em sistemas contendo íons ferro.” 2009. Thesis, Universidade de Brasília. Accessed December 09, 2019. http://bdtd.bce.unb.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=5359.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mattos, Thiago Cardoso Genaro de. “Mecanismos da ação antioxidante dos ácidos caféico e tânico em sistemas contendo íons ferro.” 2009. Web. 09 Dec 2019.

Vancouver:

Mattos TCGd. Mecanismos da ação antioxidante dos ácidos caféico e tânico em sistemas contendo íons ferro. [Internet] [Thesis]. Universidade de Brasília; 2009. [cited 2019 Dec 09]. Available from: http://bdtd.bce.unb.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=5359.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mattos TCGd. Mecanismos da ação antioxidante dos ácidos caféico e tânico em sistemas contendo íons ferro. [Thesis]. Universidade de Brasília; 2009. Available from: http://bdtd.bce.unb.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=5359

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

15. Mello, Rodrigo Brito de. Produção de biblioteca de compostos derivados de produtos naturais: síntese e estudo de atividades biológicas.

Degree: Mestrado, Produtos Naturais e Sintéticos, 2014, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/60/60138/tde-17042015-151409/ ;

►

O presente trabalho trata da semissíntese de análogos de importantes compostos líderes (afidicolina, lausona, lapachol e CAPE) utilizando técnicas de química medicinal como bioisosterismo, adição… (more)

▼

O presente trabalho trata da semissíntese de análogos de importantes compostos líderes (afidicolina, lausona, lapachol e CAPE) utilizando técnicas de química medicinal como bioisosterismo, adição de grupamento funcional e simplificação molecular. Dessa forma foi possível obter uma biblioteca de análogos racionais, visando a manipulação de parâmetros físico-químicos e estruturais, para fins de bioprospecção. Foram desenvolvidos derivados de afidicolina mais lipofílicos, por meio da acilação das hidroxilas presentes na estrutura química deste terpeno. Tentativas de formação de bioisósteros, sais e de ésteres fosfato das hidroxinaftoquinonas naturais - lapachol e lausona, foram realizadas visando avaliar a influência do pKa sobre a atividade deste tipo de moléculas, bem como aumentar hidrossolubilidade. Neste caso, foram observadas reações paralelas, como um rearranjo molecular para a formação de aminonaftoquinonas, no estudo da cicloadição de azida de sódio com grupos cianos. Ademais, foi estudado o efeito da simplificação molecular de CAPE (fenetil éster do ácido cafeico), visando entender os requisitos estruturais de atividade antitumoral desta classe de compostos. Neste trabalho, foram obtidas 14 moléculas e testadas para diferentes atividades biológicas. Derivados naftoquinoidais se mostraram ativos frente à inibição de DHODH em ensaio sobre a enzima e também em ensaio celular. Adicionalmente, análogos simplificados do CAPE apresentaram alta atividade antitumoral, com segurança, em comparação ao controle 5-fluorouracila.

The present study aimed the semi-synthesis of analogues from important lead compounds (aphidicolin, lausone, lapachol and CAPE) by using medicinal chemistry strategies, such as bioisosterism, addition of functional groups and molecular simplification. Thus, we obtained a library of rational analogues, aiming the manipulation of physicochemical and structural parameters with bioprospecting purposes. We developed more lipophilic aphidicolin derivatives by acylation of the hydroxyl groups present in the structure of this terpene. Attempts towards the development of phosphate salts bioisosters from the hydroxinaftoquinones lapachol and lausone in order to evaluate the influence the pKa in the biological activity of these class compounds as well as to increase the water solubility. In this last case, we observed parallel reactions, as a molecular rearrangement for the formation of the aminonaftoquinones during the study of cycloaddition with cyanides and azides. In addition, we studied the effects of molecular simplification of CAPE (caffeic acid phenethyl ester), to better understand the structural requirements for antitumoral activity of this class of compounds. In the present work we obtained 14 molecules which were also tested for different biological activities. Naftoquinoidais derivatives showed inhibition activity on enzymatic essay on DHODH and on cellular essay. Moreover, simplified molecules from CAPE showed high antitumoral activity and safety in comparison to the control 5- fluorouracil.

Advisors/Committee Members: Emery, Flavio da Silva.

Subjects/Keywords: Afidicolina; Aphidicolin; Bioisosterism; Bioisosterismo; Caffeic acid phenethyl ester; Fenetil éster do ácido cafeico; Molecular simplification; Naftoquinonas; Naftoquinones; Natural products; Produtos naturais; Simplificação molecular

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mello, R. B. d. (2014). Produção de biblioteca de compostos derivados de produtos naturais: síntese e estudo de atividades biológicas. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/60/60138/tde-17042015-151409/ ;

Chicago Manual of Style (16^th Edition):

Mello, Rodrigo Brito de. “Produção de biblioteca de compostos derivados de produtos naturais: síntese e estudo de atividades biológicas.” 2014. Masters Thesis, University of São Paulo. Accessed December 09, 2019. http://www.teses.usp.br/teses/disponiveis/60/60138/tde-17042015-151409/ ;.

MLA Handbook (7^th Edition):

Mello, Rodrigo Brito de. “Produção de biblioteca de compostos derivados de produtos naturais: síntese e estudo de atividades biológicas.” 2014. Web. 09 Dec 2019.

Vancouver:

Mello RBd. Produção de biblioteca de compostos derivados de produtos naturais: síntese e estudo de atividades biológicas. [Internet] [Masters thesis]. University of São Paulo; 2014. [cited 2019 Dec 09]. Available from: http://www.teses.usp.br/teses/disponiveis/60/60138/tde-17042015-151409/ ;.

Council of Science Editors:

Mello RBd. Produção de biblioteca de compostos derivados de produtos naturais: síntese e estudo de atividades biológicas. [Masters Thesis]. University of São Paulo; 2014. Available from: http://www.teses.usp.br/teses/disponiveis/60/60138/tde-17042015-151409/ ;

University of Otago

16. Ou, Zong-Quan. Pre-formulation of antioxidant phytochemicals from Sonchus oleraceus L. (pūhā) .

Degree: 2014, University of Otago

URL: http://hdl.handle.net/10523/4795

► Background Consumption of vegetables and fruits has been associated with the prevention of various diseases caused by oxidative damage due to the abundant antioxidants these… (more)

▼ Background Consumption of vegetables and fruits has been associated with the prevention of various diseases caused by oxidative damage due to the abundant antioxidants these dietary components contain. Pūhā (Sonchus oleraceus L.) leaves have been reported to be rich in antioxidants and possess strong antioxidant activity; thus show potential to be formulated into nutritional supplements. However, comprehensive investigation of antioxidant activity of individual compounds in leaf extracts from the genus Sonchus has not been reported. In this thesis the key antioxidants in pūhā have been identified. The effects of sample preparation including leaf position, harvest time, handling and extraction conditions on the key antioxidants in pūhā leaf extracts have been investigated. Stability of the antioxidants in pūhā extracts under storage, which would affect the quality of the final product, was also studied. Finally, the cellular activity of pūhā extracts was determined in terms of cellular antioxidant activity and anti-aging effects. Methods Pūhā leaf extracts were prepared in 70% methanol in water (w/w). An online method using high pressure liquid chromatography (HPLC) to separate herbal extracts followed by post-column reaction with the 2, 2’-diphenylpicrylhydrazyl (DPPH) reagent, as a measure of antioxidant activity, was applied to screen individual peaks with antioxidant activity. These active peaks of interest were isolated through flash chromatography and semi-preparative HPLC; and subsequently, structurally identified using nuclear magnetic resonance (NMR) and mass spectrometry (MS). This online method was used for monitoring the changes of the key antioxidants in pūhā extracts during sample preparations and storage. Design of experiments was applied to investigate the stability of the key antioxidants in the pūhā (leaf or extract) stored at different temperatures (4, 25 and 50°C) and relative humidities (15, 43 and 75%) for 180 days. Antioxidant activity was assessed by both the DPPH free radical assay and the Cellular Antioxidant Activity (CAA) assay with HepG2 cells. Senescence-associated β-galactosidase (SA-β-Gal) activity, a biomarker for cellular aging, was used to determine the anti-aging responses of pūhā extracts to H₂O₂ stress in WI-38 cells. Results Three key antioxidants were determined via the online method and identified as caftaric acid, chlorogenic acid and chicoric acid, with chicoric acid being the dominant compound, using MS and NMR. The antioxidant activity assessed by the online HPLC-DPPH assay was consistent with an offline DPPH assay. The highest concentration of chlorogenic acid was found in young leaves at the top of the plant; while middle leaves contained the highest total amount of antioxidant compounds. The three antioxidants degraded to unquantifiable levels after oven-drying. More than 90% of the antioxidants were retained by freeze-drying and air-drying. Pre-treatment with liquid N₂, increasing the extraction… Advisors/Committee Members: McDowell, Arlene (advisor).

Subjects/Keywords: antioxidants; Caffeic acid derivatives; Cellular Antioxidant Activity (CAA); HPLC-DPPH; DOE; Anti-ageing; <; i>; Sonchus oleraceus<; /i>; L. (pūhā)

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ou, Z. (2014). Pre-formulation of antioxidant phytochemicals from Sonchus oleraceus L. (pūhā) . (Doctoral Dissertation). University of Otago. Retrieved from http://hdl.handle.net/10523/4795

Chicago Manual of Style (16^th Edition):

Ou, Zong-Quan. “Pre-formulation of antioxidant phytochemicals from Sonchus oleraceus L. (pūhā) .” 2014. Doctoral Dissertation, University of Otago. Accessed December 09, 2019. http://hdl.handle.net/10523/4795.

MLA Handbook (7^th Edition):

Ou, Zong-Quan. “Pre-formulation of antioxidant phytochemicals from Sonchus oleraceus L. (pūhā) .” 2014. Web. 09 Dec 2019.

Vancouver:

Ou Z. Pre-formulation of antioxidant phytochemicals from Sonchus oleraceus L. (pūhā) . [Internet] [Doctoral dissertation]. University of Otago; 2014. [cited 2019 Dec 09]. Available from: http://hdl.handle.net/10523/4795.

Council of Science Editors:

Ou Z. Pre-formulation of antioxidant phytochemicals from Sonchus oleraceus L. (pūhā) . [Doctoral Dissertation]. University of Otago; 2014. Available from: http://hdl.handle.net/10523/4795

Université Paris-Sud – Paris XI

17. Bouvier d'yvoire, Madeleine. Etude de la voie de biosynthese des monolignols chez brachypodium distachyon : Identification of genes involved in the biosynthesis of monolignols in Brachypodium distachyon.

Degree: Docteur es, Sciences de la vie, 2011, Université Paris-Sud – Paris XI

URL: http://www.theses.fr/2011PA112347

►

La récente définition de Brachypodium distachyon comme modèle des graminées en fait un organisme de choix pour l’étude de leur paroi cellulaire, en particulier dans… (more)

▼

La récente définition de Brachypodium distachyon comme modèle des graminées en fait un organisme de choix pour l’étude de leur paroi cellulaire, en particulier dans le cadre de leur utilisation comme matière première renouvelable pour le bioéthanol de seconde génération. Les lignines, dont les trois unités (H, G et S) proviennent de la polymérisation des monolignols, sont associées aux acides hydroxycinnamiques dans la paroi des céréales et représentent l’obstacle majeur à l’exploitation industrielle de la biomasse lignocellulosique. L’acquisition de connaissances sur les mécanismes dirigeant leur mise en place et leur organisation permettrait d’identifier des facteurs modulant les rendements de production qui y sont associés. Quatre familles de gènes ont été étudiées et l’implication dans la voie de biosynthèse des monolignols de trois gènes a été montrée : BdF5H2 possède une activité férulate-5-hydroxylase permettant la synthèse des précurseurs des unités S des lignines, BdCOMT3 est l’isoforme principale des acide cafféique O-Méthyltransférases et sa perte partielle de fonction cause une diminution de la quantité de lignine, la modification du rapport S/G et une baisse de quantité d’acide p-coumarique dans deux lignées mutantes indépendantes. Enfin, BdCAD1 est l’isoforme principale des alcools cinnamylique déshydrogénases : sa perte de fonction dans deux lignées indépendantes cause la diminution de la quantité globale de lignine et d’acide p-coumarique, une baisse du rapport S/G ainsi que l’accumulation de sinapaldéhyde. Par ailleurs ces deux lignées présentent des rendements de saccharification augmentés de plus d’un quart par rapport au sauvage.

Brachypodium distachyon was recently adopted as an experimental model for grass species. As such, it is used to study grass cell wall, in particular in the context of their use as renewable feedstock for the production of second generation bioethanol. Lignins are polymers of three main units (H, G and S) originating from the polymerization of monolignols, and are linked to hydroxycinnamic acids in grasses. They constitute the main bottleneck to industrial processes targeting lignocellulosic biomass and improving the understanding of the mechanisms directing their structure and deposition could lead to the identification of the factors modulating associated production yields. Four gene families were studied and the involvement of three genes in the monolignols biosynthetic pathway was shown: BdF5H2 displays a ferulate-5-hydroxylase activity enabling the synthesis of the S lignin units, BdCOMT3 is the main caffeic acid O-methyltransferase and its partial loss of function in two independent mutant lines leads to the reduction of lignin content, the modification of the S/G units ratio and a decrease in p-coumaric acid accumulation. BdCAD1 is the main cinnamyl alcohol dehydrogenase isoform: its loss of function in two independent mutant lines results in a decrease in lignin content and of the S/G ratio and the accumulation of sinapaldehyde. Moreover, these two lines display…

Advisors/Committee Members: Jouanin, Lise (thesis director), Lapierre, Catherine (thesis director).

Subjects/Keywords: Brachypodium distachyon; Lignine; Monolignol; Acide cafféique O-Méthyltransférase; Alcool cinnamylique déshydrogénase; Férulate-5-hydroxylase; Saccharification; Brachypodium distachyon; Lignin; Monolignol; Caffeic acid O-methyltransferase; Cinnamyl alcohol dehydrogenase; Ferulate-5-hydroxylase; Saccharification

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bouvier d'yvoire, M. (2011). Etude de la voie de biosynthese des monolignols chez brachypodium distachyon : Identification of genes involved in the biosynthesis of monolignols in Brachypodium distachyon. (Doctoral Dissertation). Université Paris-Sud – Paris XI. Retrieved from http://www.theses.fr/2011PA112347

Chicago Manual of Style (16^th Edition):

Bouvier d'yvoire, Madeleine. “Etude de la voie de biosynthese des monolignols chez brachypodium distachyon : Identification of genes involved in the biosynthesis of monolignols in Brachypodium distachyon.” 2011. Doctoral Dissertation, Université Paris-Sud – Paris XI. Accessed December 09, 2019. http://www.theses.fr/2011PA112347.

MLA Handbook (7^th Edition):

Bouvier d'yvoire, Madeleine. “Etude de la voie de biosynthese des monolignols chez brachypodium distachyon : Identification of genes involved in the biosynthesis of monolignols in Brachypodium distachyon.” 2011. Web. 09 Dec 2019.

Vancouver:

Bouvier d'yvoire M. Etude de la voie de biosynthese des monolignols chez brachypodium distachyon : Identification of genes involved in the biosynthesis of monolignols in Brachypodium distachyon. [Internet] [Doctoral dissertation]. Université Paris-Sud – Paris XI; 2011. [cited 2019 Dec 09]. Available from: http://www.theses.fr/2011PA112347.

Council of Science Editors:

Bouvier d'yvoire M. Etude de la voie de biosynthese des monolignols chez brachypodium distachyon : Identification of genes involved in the biosynthesis of monolignols in Brachypodium distachyon. [Doctoral Dissertation]. Université Paris-Sud – Paris XI; 2011. Available from: http://www.theses.fr/2011PA112347

18. Galani, Anastasia. Σύνθεση συµπλόκων στοιχείων μετάπτωσης µε βιολογικής σηµασίας μόρια: μελέτη της αντικαρκινικής και αντιδιαβητικής τους δράσης.

Degree: 2015, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ)

URL: http://hdl.handle.net/10442/hedi/44051

►

The subject of the present doctoral thesis concerns the study ofsome transition metals with chelating agents that are of biological andpharmacological interest. Particularly, the reaction… (more)

▼

The subject of the present doctoral thesis concerns the study ofsome transition metals with chelating agents that are of biological andpharmacological interest. Particularly, the reaction of copper, zinc,molybdenum and vanadium with the ligands levofloxacin,1.10phenanthroline, 2.2’-bipyridine, carnitine, malonic acid and caffeic acid isinvestigated. These ligands are very important because of their therapeuticapplications. In addition the aforementioned transition metals are essentialnutrients for humans and plants participating in many biological functions.Quinolones are a class of synthetic drugs which are derivatives ofthe 4-quinolone, and are known for their antibacterial activity mainly due tothe inhibition of formation of deoxyribonucleic acid. In this context isreferred, the study and characterization by elemental analysis and variousspectroscopic methods (IR, UV-Vis, EPR, NMR, TGA) of complexes oflevofloxacin. Complexes of the third generation fluoroquinolone,levofloxacin, were prepared in the presence and absence of heterocyclicsubstituents donor nitrogen atoms such as 2.2’-bipyridine and 1.10-phenanthroline. In some of them analysis of their structure by X-raydiffraction, was followed. Furthermore it was studied the interaction ofthese complexes with DNA and the antibacterial activity against threedifferent microorganisms, Escherichia Coli, Pseudomonas Aeruginosa andStaphylococcus Aureus. In addition their cytotoxic activity was evaluatedagainst MCF-7 cell line. Furthermore, experiments of confocal fluorescentwere performed for the zinc complexes of levofloxacin.Carnitine is a molecule involved in biological oxidations. Furthermore,it is known for its anticancer and antidiabetic activity like malonate andcaffeic acid molecules. In this context, the synthesis, study and characterization by elemental analysis and various spectroscopic methods(IR, UV-Vis, NMR, TGA) of complexes of the above ligands is refered. Insome of them crystal structure analysis was performed, by X-ray diffractionwhile determination of their anticancer and antidiabetic activity wasperformed. Furthermore, experiments of confocal fluorescent wereperformed for the complexes of carnitine.

Το αντικείµενο της παρούσας διδακτορικής διατριβής αφορά την µελέτη συµπλόκων ενώσεων του χαλκού, του ψευδαργύρου, του µολυβδαινίου και του βαναδίου µε µόρια βιολογικής και φαρµακολογικής σηµασίας. Συγκεκριµένα µελετάται η σύνθεση, ο χαρακτηρισµός και η ταυτοποίηση συµπλόκων ενώσεων του χαλκού, ψευδαργύρου,µολυβδαινίου και βαναδίου µε τους υποκαταστάτες λεβοφλοξασίνη, 1.10-φαινανθρολίνη, 2.2΄-διπυριδίνη, καρνιτίνη, µαλονικό οξύ και καφεϊκό οξύ. Οι υποκαταστάτες αυτοί είναι σηµαντικοί διότι εµπλέκονται σε βιολογικές διεργασίες και έχουν φαρµακολογικές εφαρµογές. Τα προαναφερθέντα στοιχεία µετάπτωσης, είναι απαραίτητα για τον άνθρωπο και τα φυτά, συµµετέχοντας σε πολλές βιολογικές δράσεις. Οι κινολόνες είναι µια κατηγορία συνθετικών φαρµάκων, τα οποία είναι παράγωγα της 4-κινολόνης, και είναι γνωστά για την αντιβακτηριακή τους δράση κυρίως λόγω της…

Subjects/Keywords: Λεβοφλοξασίνη.; 1.10-φαινανθρολίνη; 2.2΄-διπυριδίνη; Αντιμικροβιακή δράση; Αντικαρκινική δράση; Καρνιτίνη; Αντιδιαβητική δράση; Μαλονικό οξύ; Καφεϊκό οξύ; Κυτταροτοξικότητα; Φαινανθρολίνη; Διπυριδίνη; Μικροσκοπία φθοριµού; Φθορισμός; Levofloxacin; 1.10-phenanthroline; 2.2’-bipyridine; Antimicrobial activity; Anticancer activity; Carnitine; Antidiabetic activity; Malonic acid; Caffeic acid; DNA; MIC; Cytitoxicity; Phenanthroline; Bipyridines; Confocal fluorescent

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Galani, A. (2015). Σύνθεση συµπλόκων στοιχείων μετάπτωσης µε βιολογικής σηµασίας μόρια: μελέτη της αντικαρκινικής και αντιδιαβητικής τους δράσης. (Thesis). National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Retrieved from http://hdl.handle.net/10442/hedi/44051

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Galani, Anastasia. “Σύνθεση συµπλόκων στοιχείων μετάπτωσης µε βιολογικής σηµασίας μόρια: μελέτη της αντικαρκινικής και αντιδιαβητικής τους δράσης.” 2015. Thesis, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Accessed December 09, 2019. http://hdl.handle.net/10442/hedi/44051.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Galani, Anastasia. “Σύνθεση συµπλόκων στοιχείων μετάπτωσης µε βιολογικής σηµασίας μόρια: μελέτη της αντικαρκινικής και αντιδιαβητικής τους δράσης.” 2015. Web. 09 Dec 2019.

Vancouver:

Galani A. Σύνθεση συµπλόκων στοιχείων μετάπτωσης µε βιολογικής σηµασίας μόρια: μελέτη της αντικαρκινικής και αντιδιαβητικής τους δράσης. [Internet] [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2015. [cited 2019 Dec 09]. Available from: http://hdl.handle.net/10442/hedi/44051.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Galani A. Σύνθεση συµπλόκων στοιχείων μετάπτωσης µε βιολογικής σηµασίας μόρια: μελέτη της αντικαρκινικής και αντιδιαβητικής τους δράσης. [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2015. Available from: http://hdl.handle.net/10442/hedi/44051

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of the Western Cape

19. Klein, Ashwil Johan. Modulation of soybean and maize antioxidant activities by caffeic acid and nitric oxide under salt stress .

Degree: 2012, University of the Western Cape

URL: http://hdl.handle.net/11394/3595

Subjects/Keywords: Nitric oxide; Salinity; Reactive oxygen species; Cell death; Antioxidant enzymes; Caffeic acid; Lipid peroxidation; Superoxide; Antioxidant gene expression; Salt stress tolerance

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Klein, A. J. (2012). Modulation of soybean and maize antioxidant activities by caffeic acid and nitric oxide under salt stress . (Thesis). University of the Western Cape. Retrieved from http://hdl.handle.net/11394/3595

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Klein, Ashwil Johan. “Modulation of soybean and maize antioxidant activities by caffeic acid and nitric oxide under salt stress .” 2012. Thesis, University of the Western Cape. Accessed December 09, 2019. http://hdl.handle.net/11394/3595.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Klein, Ashwil Johan. “Modulation of soybean and maize antioxidant activities by caffeic acid and nitric oxide under salt stress .” 2012. Web. 09 Dec 2019.

Vancouver:

Klein AJ. Modulation of soybean and maize antioxidant activities by caffeic acid and nitric oxide under salt stress . [Internet] [Thesis]. University of the Western Cape; 2012. [cited 2019 Dec 09]. Available from: http://hdl.handle.net/11394/3595.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Klein AJ. Modulation of soybean and maize antioxidant activities by caffeic acid and nitric oxide under salt stress . [Thesis]. University of the Western Cape; 2012. Available from: http://hdl.handle.net/11394/3595

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universitetet i Tromsø

20. Moriana Armendariz, Mikel. Yearly variation in allelopathic compound production along a climatic gradient. A case of study of Empetrum nigrum .

Degree: 2017, Universitetet i Tromsø

URL: http://hdl.handle.net/10037/16668

► Empetrum nigrum is a plant common in northern ecosystems with capacity to produce allelopathic compounds, which among other effects inhibit seed establishment and germination of… (more)

Subjects/Keywords: Empetrum nigrum; allelopathy; batatasin-III; caffeic acid; antioxidant activity; plant growth; weather effects; VDP::Mathematics and natural science: 400::Zoology and botany: 480::Plant physiology: 492; VDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480::Plantefysiologi: 492

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Moriana Armendariz, M. (2017). Yearly variation in allelopathic compound production along a climatic gradient. A case of study of Empetrum nigrum . (Masters Thesis). Universitetet i Tromsø. Retrieved from http://hdl.handle.net/10037/16668

Chicago Manual of Style (16^th Edition):

Moriana Armendariz, Mikel. “Yearly variation in allelopathic compound production along a climatic gradient. A case of study of Empetrum nigrum .” 2017. Masters Thesis, Universitetet i Tromsø. Accessed December 09, 2019. http://hdl.handle.net/10037/16668.

MLA Handbook (7^th Edition):

Moriana Armendariz, Mikel. “Yearly variation in allelopathic compound production along a climatic gradient. A case of study of Empetrum nigrum .” 2017. Web. 09 Dec 2019.

Vancouver:

Moriana Armendariz M. Yearly variation in allelopathic compound production along a climatic gradient. A case of study of Empetrum nigrum . [Internet] [Masters thesis]. Universitetet i Tromsø 2017. [cited 2019 Dec 09]. Available from: http://hdl.handle.net/10037/16668.

Council of Science Editors:

Moriana Armendariz M. Yearly variation in allelopathic compound production along a climatic gradient. A case of study of Empetrum nigrum . [Masters Thesis]. Universitetet i Tromsø 2017. Available from: http://hdl.handle.net/10037/16668

21. Kitsati, Natalia. Διερεύνηση των βιοχημικών μηχανισμών δράσης των δραστικών μορφών οξυγόνου: ο ρόλος των καταλυτικά ενεργών ιόντων σιδήρου σε σχέση με τη διατροφή και την υγεία.

Degree: 2017, University of Ioannina; Πανεπιστήμιο Ιωαννίνων

URL: http://hdl.handle.net/10442/hedi/44726

►

Iron is indispensable for life but when is available in redox active form, can participate in reactions that generate extremely reactive radicals, able to attack… (more)

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Iron is indispensable for life but when is available in redox active form, can participate in reactions that generate extremely reactive radicals, able to attack and oxidize all main cellular constituents. Studies have shown that a large number of Mediterranean diet compounds protect cells in conditions of oxidative stress. In the present investigation, we evaluated the capacity of several phenolic acids, which are present in the Mediterranean diet, to protect cells from oxidative stress-induced DNA damage. It was observed that caffeic acid, protected cells from H2O2-induced DNA damage by chelating intracellular labile iron due to its ortho-dihydroxy group. Caffeic acid, which are able to chelate iron but unable to diffuse through the plasma membrane due to the negative charge of their carboxylic moiety at neutral pH, required higher concentrations to be protective. Therefore, we decided to neutralize this charge by esterication, so we synthesized chemically caffeic derivatives (esters). This change could increase the lipophilicity. The eective protection was based on the ability of each compound to (1) reach the intracellular space and (2) chelate intracellular “labile” iron. Then, we examined two major phenolic olive oil alcohols, hydroxytyrosol and tyrosol, in apoptosis induced by oxidative stress. These compounds are uncharged and have the ability to diffuse easily through the cell membrane. It was observed that hydroxytyrosol which chelate intracellular labile iron due to its ortho-dihydroxy group, was able to diminish the late sustained phase of H2O2-induced JNK and p38 phosphorylation. We also investigated the role of iron homeostasis at the end-stage renal patients undergoing maintenance hemodialysis (HD). The estimation of transferrin saturation (Tf-Sat) is not possible with traditional methodologies after intravenous administration of iron preparations, due to the presence of non-transferrin bound iron (NTBI) in the serum. Therefore, we used urea polyacrylamide electrophoresis technique (U-PAGE). With this technique, we tested Tf-Sat, differic transferrin and the expression of serum hepcidin, the principal hormone that regulates iron homeostasis. The observation that serum hepcidin levels increased in parallel with Tf-Fe2 and Tf-Sat shortly after iron administration indicates a fast signaling response, which is expected to have a negative influence on iron efflux from cells to circulation. In conclusion intracellular labile iron play pivotal role in cell protection mechanism under oxidative stress. Thus, it was rational to imagine that numerous natural phenolic compounds, which are present abundantly in the Mediterranean diet, are able to modulate intracellular labile iron homeostasis, contributing to the maintenance of human health.

Ο σίδηρος αποτελεί απαραίτητο ιχνοστοιχείο της ζωής, ωστόσο μπορεί να γίνει τοξικός για το κύτταρο συμμετέχοντας σε αντιδράσεις τύπου Fenton με δισθενή ιόντα σιδήρου, οδηγώντας στην παραγωγή ελευθέρων ριζών υδροξυλίου που οξειδώνουν όλα τα κυτταρικά συστατικά. Μελέτες έχουν…

Subjects/Keywords: Οξειδωτικό στρες; Καταλυτικά ενεργά ιόντα σιδήρου; Διατροφή; Παράγωγα καφεϊκού οξέος; Βλάβες στο DNA; Απόπτωση; Υδροξυτυροσόλη; Τυροσόλη; Μεταγωγή σήματος; MAPK κινάσες; Ενδοφλέβια χορήγηση σιδήρου; Ηπατιδίνη; Αιμοκάθαρση; Oxidative stress; Labile iron; Mediterranean diet; Caffeic acid derivatives; DNA damage; Apoptosis; Hydroxytyrosol; Tyrosol; Redox signaling; Mitogen activated protein kinases (MARKS); Intravenous iron infusion; Hepcidin; Hemodialysis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kitsati, N. (2017). Διερεύνηση των βιοχημικών μηχανισμών δράσης των δραστικών μορφών οξυγόνου: ο ρόλος των καταλυτικά ενεργών ιόντων σιδήρου σε σχέση με τη διατροφή και την υγεία. (Thesis). University of Ioannina; Πανεπιστήμιο Ιωαννίνων. Retrieved from http://hdl.handle.net/10442/hedi/44726

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kitsati, Natalia. “Διερεύνηση των βιοχημικών μηχανισμών δράσης των δραστικών μορφών οξυγόνου: ο ρόλος των καταλυτικά ενεργών ιόντων σιδήρου σε σχέση με τη διατροφή και την υγεία.” 2017. Thesis, University of Ioannina; Πανεπιστήμιο Ιωαννίνων. Accessed December 09, 2019. http://hdl.handle.net/10442/hedi/44726.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kitsati, Natalia. “Διερεύνηση των βιοχημικών μηχανισμών δράσης των δραστικών μορφών οξυγόνου: ο ρόλος των καταλυτικά ενεργών ιόντων σιδήρου σε σχέση με τη διατροφή και την υγεία.” 2017. Web. 09 Dec 2019.

Vancouver:

Kitsati N. Διερεύνηση των βιοχημικών μηχανισμών δράσης των δραστικών μορφών οξυγόνου: ο ρόλος των καταλυτικά ενεργών ιόντων σιδήρου σε σχέση με τη διατροφή και την υγεία. [Internet] [Thesis]. University of Ioannina; Πανεπιστήμιο Ιωαννίνων; 2017. [cited 2019 Dec 09]. Available from: http://hdl.handle.net/10442/hedi/44726.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kitsati N. Διερεύνηση των βιοχημικών μηχανισμών δράσης των δραστικών μορφών οξυγόνου: ο ρόλος των καταλυτικά ενεργών ιόντων σιδήρου σε σχέση με τη διατροφή και την υγεία. [Thesis]. University of Ioannina; Πανεπιστήμιο Ιωαννίνων; 2017. Available from: http://hdl.handle.net/10442/hedi/44726

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Brno University of Technology

22. Michalíček, Jan. Stanovení obsahových látek rostliny Echinacea .

Degree: 2008, Brno University of Technology

URL: http://hdl.handle.net/11012/12881

► Rostliny rodu Echinacea jsou léčivé byliny stimulující imunitní systém. Používají se zvláště v boji proti chřipce a infekcím horních cest dýchacích. V této práci bylo… (more)

▼ Rostliny rodu Echinacea jsou léčivé byliny stimulující imunitní systém. Používají se zvláště v boji proti chřipce a infekcím horních cest dýchacích. V této práci bylo analyzováno 7 komerčních výrobků obsahujících výtažky z rostliny Echinacea vysokoúčinnou kapalinovou chromatografií. Bylo provedeno kvantitativní stanovení derivátů kyseliny kávové, jež jsou látky zodpovědné za léčivé účinky rostliny. Zjištěné obsahy kyseliny chlorogenové, kávové, cichorové a echinakosidu byly porovnány v jednotlivých výrobcích. Dále byly připraveny extrakty z kořene, listů a květů třapatky nachové pomocí extrakce za zvýšeného tlaku extrakčního rozpouštědla při různých podmínkách, aby se z obsahu účinných látek v nich přítomných zjistilo, které extrakční podmínky jsou nejlepší. Z naměřených dat bylo zjištěno, že největší celková koncentrace obsahových látek je přítomna ve výrobku Imunit Echinaceové kapky firmy Simply You. Jako nejlepší extrakční podmínky se ukázaly teplota 80 °C v kombinaci s rozpouštědlem ve složení EtOH/voda = 40/60, a to jak při extrakci kořene, tak i listů a květů.; Herbal plants belonging to the Echinacea genus are immunostimulants used especially for treatment of a common cold and upper respiratory tract infections. Seven Echinacea commercial products were analysed with high-performance liquid chromatography to quantitatively determine caffeic acid derivatives that are responsible for the plant healing properties. Determined contents of chlorogenic acid, caffeic acid, cichoric acid and echinacoside were compared between individual samples. Root and leaf-bloom extracts of Echinacea purpurea were prepared with pressurized solvent extraction under different conditions to show, which solvent system and temperarure is the most proper for extracting the highest amount of caffeic acid derivatives. From the measured data, Imunit Echinaceove kapky from Simply You was chosen as the product with the highest total concentation of the content compounds. Extraction at 80 °C using EtOH/water = 40/60 was found as the best way giving the highest concentration of caffeic acid derivatives from roots as well as leaves and blooms. Advisors/Committee Members: Zemanová, Jana (advisor).

Subjects/Keywords: rod Echinacea; vysokoúčinná kapalinová chromatografie; obsahové látky; deriváty kyseliny kávové; echinakosid; Echinacea species; high-performance liquid chromatography; content compounds; caffeic acid derivatives; echinacoside

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❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Michalíček, J. (2008). Stanovení obsahových látek rostliny Echinacea . (Thesis). Brno University of Technology. Retrieved from http://hdl.handle.net/11012/12881

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Michalíček, Jan. “Stanovení obsahových látek rostliny Echinacea .” 2008. Thesis, Brno University of Technology. Accessed December 09, 2019. http://hdl.handle.net/11012/12881.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Michalíček, Jan. “Stanovení obsahových látek rostliny Echinacea .” 2008. Web. 09 Dec 2019.

Vancouver:

Michalíček J. Stanovení obsahových látek rostliny Echinacea . [Internet] [Thesis]. Brno University of Technology; 2008. [cited 2019 Dec 09]. Available from: http://hdl.handle.net/11012/12881.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Michalíček J. Stanovení obsahových látek rostliny Echinacea . [Thesis]. Brno University of Technology; 2008. Available from: http://hdl.handle.net/11012/12881

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

23. Humphrey, Peter Saah. Signal Transduction Mechanisms for Stem Cell Differentation Into Cardiomyocytes .

Degree: 2009, University of Hertfordshire

URL: http://hdl.handle.net/2299/3760

► Cardiovascular diseases are among the leading causes of death worldwide and particularly in the developed World. The search for new therapeutic approaches for improving the… (more)

▼ Cardiovascular diseases are among the leading causes of death worldwide and particularly in the developed World. The search for new therapeutic approaches for improving the functions of the damaged heart is therefore a critical endeavour. Myocardial infarction, which can lead to heart failure, is associated with irreversible loss of functional cardiomyocytes. The loss of cardiomyocytes poses a major difficulty for treating the damaged heart since terminally differentiated cardiomyocytes have very limited regeneration potential. Currently, the only effective treatment for severe heart failure is heart transplantation but this option is limited by the acute shortage of donor hearts. The high incidence of heart diseases and the scarcity donor hearts underline the urgent need to find alternative therapeutic approaches for treating cardiovascular diseases. Pluripotent embryonic stem (ES) cells can differentiate into functional cardiomyocytes. Therefore the engraftment of ES cell-derived functional cardiomyocytes or cardiac progenitor cells into the damaged heart to regenerate healthy myocardial tissues may be used to treat damaged hearts. Stem cell-based therapy therefore holds a great potential as a very attractive alternative to heart transplant for treating heart failure and other cardiovascular diseases. A major obstacle to the realisation of stem cell-based therapy is the lack of donor cells and this in turn is due to the fact that, currently, the molecular mechanisms or the regulatory signal transduction mechanisms that are responsible for mediating ES cell differentiation into cardiomyocytes are not well understood. Overcoming this huge scientific challenge is absolutely necessary before the use of stem cell-derived cardiomyocytes to treat the damaged heart can become a reality. Therefore the aim of this thesis was to investigate the signal transduction pathways that are involved in the differentiation of stem cells into cardiomyocytes. The first objective was the establishment and use of cardiomyocyte differentiation models using H9c2 cells and P19 stem cells to accomplish the specific objectives of the thesis. The specific objectives of the thesis were, the investigation of the roles of (i) nitric oxide (ii) protein kinase C (PKC), (iii) p38 mitogen-activated protein kinase (p38 MAPK) (vi) phosphoinositide 3-kinase (PI3K) and (vi) nuclear factor-kappa B (NF-kB) signalling pathways in the differentiation of stem cells to cardiomyocytes and, more importantly, to identify where possible any points of convergence and potential cross-talk between pathways that may be critical for differentiation to occur. P19 cells were routinely cultured in alpha minimal essential medium (??-MEM) supplemented with 100 units/ml penicillin /100 ??g/ml streptomycin and 10% foetal bovine serum (FBS). P19 cell differentiation was initiated by culturing the cells in microbiological plates in medium containing 0.8 % DMSO to form embryoid bodies (EB). This was followed by transfer of EBs to cell culture grade dishes after four…

Subjects/Keywords: Akt; Protein Kinase B; Bisindolylmaleimide I; 8-Bromo-cGMP; Caffeic Acid Phenethyl Ester; CAPE; Cardiomyocytes; Differentiation; Embryonic Stem Cells; H9c2 (2-1); Ly294002; Myosin Chain Light Chain 1; MLC; Myosin Heavy Chain; MHC; Nanog; Nitric Oxide; Nkx2.5; NOC-18; NOC-5; Nuclear Factor-Kappa B; NF-kB; P19 Embryonal Carcinoma Cell; P19 Stem Cells; P38 MAPK; P38 Mitogen-Activated Protein Kinase; Phosphoinositide 3-Kinase; PI3K; Protein Kinase C; PKC; SB203580; Signal Transduction; Signalling; SIN-1; SNAP; Sox2; Transcription Factors; Troponin I; Wnt Proteins; GATA; Bone Morphogenetic Proteins; Leukaemia Inhibitory Factor; Oct-4; Octamer-Binding Protein

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Humphrey, P. S. (2009). Signal Transduction Mechanisms for Stem Cell Differentation Into Cardiomyocytes . (Thesis). University of Hertfordshire. Retrieved from http://hdl.handle.net/2299/3760

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Humphrey, Peter Saah. “Signal Transduction Mechanisms for Stem Cell Differentation Into Cardiomyocytes .” 2009. Thesis, University of Hertfordshire. Accessed December 09, 2019. http://hdl.handle.net/2299/3760.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Humphrey, Peter Saah. “Signal Transduction Mechanisms for Stem Cell Differentation Into Cardiomyocytes .” 2009. Web. 09 Dec 2019.

Vancouver:

Humphrey PS. Signal Transduction Mechanisms for Stem Cell Differentation Into Cardiomyocytes . [Internet] [Thesis]. University of Hertfordshire; 2009. [cited 2019 Dec 09]. Available from: http://hdl.handle.net/2299/3760.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Humphrey PS. Signal Transduction Mechanisms for Stem Cell Differentation Into Cardiomyocytes . [Thesis]. University of Hertfordshire; 2009. Available from: http://hdl.handle.net/2299/3760

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

ETH Zürich

24. Schär, Aline Lea. Enzymatic Synthesis and Hydrolysis of Linear Alkyl and Steryl Hydroxycinnamic Acid Esters.

Degree: 2016, ETH Zürich

URL: http://hdl.handle.net/20.500.11850/155731

Subjects/Keywords: NEBENPRODUKTE UND ABFÄLLE DER MÜLLEREI (GETREIDETECHNOLOGIE); DIHYDROXYZIMTSÄUREN + FERULASÄURE + KAFFEESÄURE + HESPERANTINSÄURE + HERNIARIN (ALIPHATISCHE UNGESÄTTIGTE CARBONSÄUREN); ENZYMATISCHE SYNTHESE + ENZYMATISCHE REAKTIONEN (ORGANISCHE CHEMIE); VERESTERUNG + UMESTERUNG (CHEMISCHE REAKTIONEN); PHYSIOLOGISCHE WIRKUNGEN VON ANTIOXIDANTIEN; BY-PRODUCTS AND WASTES FROM MILLERY (CEREAL TECHNOLOGY); DIHYDROXYCINNAMIC ACIDS + FERULIC ACID + HESPERANTIC ACID + CAFFEIC ACID + HERNIARIN (ALIPHATIC UNSATURATED CARBOXYLIC ACIDS); ENZYMATIC SYNTHESIS + ENZYMATIC REACTIONS (ORGANIC CHEMISTRY); ESTERIFICATION + TRANSESTERIFICATION (CHEMICAL REACTIONS); PHYSIOLOGICAL EFFECTS OF ANTIOXIDANTS; info:eu-repo/classification/ddc/580; info:eu-repo/classification/ddc/660; Botanical sciences; Chemical engineering

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Schär, A. L. (2016). Enzymatic Synthesis and Hydrolysis of Linear Alkyl and Steryl Hydroxycinnamic Acid Esters. (Doctoral Dissertation). ETH Zürich. Retrieved from http://hdl.handle.net/20.500.11850/155731

Chicago Manual of Style (16^th Edition):

Schär, Aline Lea. “Enzymatic Synthesis and Hydrolysis of Linear Alkyl and Steryl Hydroxycinnamic Acid Esters.” 2016. Doctoral Dissertation, ETH Zürich. Accessed December 09, 2019. http://hdl.handle.net/20.500.11850/155731.

MLA Handbook (7^th Edition):

Schär, Aline Lea. “Enzymatic Synthesis and Hydrolysis of Linear Alkyl and Steryl Hydroxycinnamic Acid Esters.” 2016. Web. 09 Dec 2019.

Vancouver:

Schär AL. Enzymatic Synthesis and Hydrolysis of Linear Alkyl and Steryl Hydroxycinnamic Acid Esters. [Internet] [Doctoral dissertation]. ETH Zürich; 2016. [cited 2019 Dec 09]. Available from: http://hdl.handle.net/20.500.11850/155731.

Council of Science Editors:

Schär AL. Enzymatic Synthesis and Hydrolysis of Linear Alkyl and Steryl Hydroxycinnamic Acid Esters. [Doctoral Dissertation]. ETH Zürich; 2016. Available from: http://hdl.handle.net/20.500.11850/155731

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