subject:(bovine oocyte)

Louisiana State University

1. Farmer, Sarah. Regulation of oocyte meiotic resumption using cAMP modulators in bovine in vitro maturation.

Degree: MS, Animal Sciences, 2014, Louisiana State University

URL: etd-04042014-085932 ; https://digitalcommons.lsu.edu/gradschool_theses/3977

► In vitro maturation (IVM) is a reproductive technique critical to in vitro embryo production (IVP) in commercial livestock industries, research, and human infertility treatment. Currently,… (more)

▼ In vitro maturation (IVM) is a reproductive technique critical to in vitro embryo production (IVP) in commercial livestock industries, research, and human infertility treatment. Currently, IVP has low efficiency due to an inadequate IVM system in which premature meiotic resumption results in low oocyte viability. Meiotic arrest is regulated primarily by 3’,5’-cyclic adenosine monophosphate (cAMP), and the most successful methods of improving IVM utilize cAMP modulators to maintain high intra-oocyte cAMP, delaying the onset of maturation. This thesis includes experiments comparing standard bovine IVM to a novel extended IVM method similar to the procedure described by Albuz and colleagues (Albuz et al., 2010). Bovine oocytes were obtained from mixed breed cattle by transvaginal ultrasound-guided aspiration. Oocytes from each cow were divided into two groups: standard IVM and extended IVM. Standard IVM consists of a 23-hour maturation composed of TCM-199 based media supplemented with 10% fetal bovine serum, sodium pyruvate, pen/strep, glutamine, and FSH, and cultured in 5% CO2 at 39⁰C. Extended IVM is composed of two steps: a pre-IVM of HEPES-TALP supplemented with 100 µM forskolin (FSK) and 500 µM 3-isobutyl-1-methylxanthine (IBMX) for 2 hours at 39⁰C, and then an extended IVM consisting of standard maturation medium supplemented with 20 µM cilostamide for 31 hours (5% CO2, 39⁰C). Oocytes were sampled at various times throughout maturation depending on the experiment. Data was collected either by staining with aceto-orcein to determine nuclear status or by a cAMP ELISA after freezing in groups of ten. Data from the initial experiments showed that cAMP modulators significantly delayed maturation, but overall maturation rates were significantly less than standard IVM (44.5% vs. 81%). Results of the cAMP assay indicated a significant increase in cAMP within the first three hours of oocyte collection after using FSK and IBMX in collection media, but cAMP was not maintained in the cilostamide-only extended IVM medium. Additionally, cilostamide may have had a negative effect on the oocytes since there was a higher percentage arrested at MI in extended IVM.

Subjects/Keywords: cAMP modulators; bovine; oocyte maturation

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APA (6^th Edition):

Farmer, S. (2014). Regulation of oocyte meiotic resumption using cAMP modulators in bovine in vitro maturation. (Masters Thesis). Louisiana State University. Retrieved from etd-04042014-085932 ; https://digitalcommons.lsu.edu/gradschool_theses/3977

Chicago Manual of Style (16^th Edition):

Farmer, Sarah. “Regulation of oocyte meiotic resumption using cAMP modulators in bovine in vitro maturation.” 2014. Masters Thesis, Louisiana State University. Accessed January 22, 2021. etd-04042014-085932 ; https://digitalcommons.lsu.edu/gradschool_theses/3977.

MLA Handbook (7^th Edition):

Farmer, Sarah. “Regulation of oocyte meiotic resumption using cAMP modulators in bovine in vitro maturation.” 2014. Web. 22 Jan 2021.

Vancouver:

Farmer S. Regulation of oocyte meiotic resumption using cAMP modulators in bovine in vitro maturation. [Internet] [Masters thesis]. Louisiana State University; 2014. [cited 2021 Jan 22]. Available from: etd-04042014-085932 ; https://digitalcommons.lsu.edu/gradschool_theses/3977.

Council of Science Editors:

Farmer S. Regulation of oocyte meiotic resumption using cAMP modulators in bovine in vitro maturation. [Masters Thesis]. Louisiana State University; 2014. Available from: etd-04042014-085932 ; https://digitalcommons.lsu.edu/gradschool_theses/3977

University of Guelph

2. Gilchrist, Graham. Dynamics of Telomeres and MicroRNAs During Oocyte Maturation and Embryo Development in the Cow.

Degree: PhD, Department of Biomedical Sciences, 2015, University of Guelph

URL: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/9068

► Successful fertilization and subsequent embryo development rely on complex molecular processes including genetic reprogramming and chromatin remodelling. During the embryonic genome activation through the early… (more)

▼ Successful fertilization and subsequent embryo development rely on complex molecular processes including genetic reprogramming and chromatin remodelling. During the embryonic genome activation through the early cleavage stages of embryo development, telomere-reprogramming events occur to maintain genomic stability for the organism through continuous mitotic divisions. Telomeres are nucleoprotein complexes that consist of a repeat nucleotide sequence and proteins that make up the shelterin complex that bind to telomeric repeats and increase genomic stability at the chromosome ends. The mechanisms that regulate telomere biology in bovine oocytes and embryos remain uncharacterized. MicroRNAs are small non-coding RNA molecules that post-transcriptionally regulate gene expression by either repressing the translation, or targeting transcripts for degradation. MicroRNAs have been previously reported to regulate important molecular functions in various cell types and have been shown to be present in bovine oocytes and embryos. It was therefore hypothesized that small RNAs expressed during maturation and development in bovine oocytes and embryos participate in the regulation of telomere biology. Examining the dynamics of telomere reprogramming in the developing bovine embryo through qPCR assays revealed an increase in telomerase activity and telomere length in blastocyst stage embryos and a decrease in the expression of one of the primary telomere related proteins, TERF2. Small RNA sequencing was then employed to characterize the miRNA population present in oocytes but did not reveal miRNA candidates with the potential to participate in telomere regulation in GV or MII oocytes or zygote stages. However, microRNA expression patterns were observed to be markedly different between stages, to correlate well with the bovine proteome at the same stages and with predicted transcript targets involved in other important signalling pathways required for oocyte competence and fertilization. Significant increases in the expression of miR-155, miR-222, miR-21, and let-7d were noted, while decreases in miR-190 and several other miRNAs were observed, although these did not correlate with telomere biology in the oocyte. Importantly, miR-148a was both abundant and relatively static throughout oocyte maturation. Finally, selective transcriptional activation of several primary-microRNAs highlights the importance of miRNAs in the molecular control of oocyte and embryo biology in cattle. Advisors/Committee Members: LaMarre, Jonathan (advisor), King, W. Allan (advisor).

Subjects/Keywords: microRNA; telomeres; oocyte maturation; embryo development; bovine

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APA (6^th Edition):

Gilchrist, G. (2015). Dynamics of Telomeres and MicroRNAs During Oocyte Maturation and Embryo Development in the Cow. (Doctoral Dissertation). University of Guelph. Retrieved from https://atrium.lib.uoguelph.ca/xmlui/handle/10214/9068

Chicago Manual of Style (16^th Edition):

Gilchrist, Graham. “Dynamics of Telomeres and MicroRNAs During Oocyte Maturation and Embryo Development in the Cow.” 2015. Doctoral Dissertation, University of Guelph. Accessed January 22, 2021. https://atrium.lib.uoguelph.ca/xmlui/handle/10214/9068.

MLA Handbook (7^th Edition):

Gilchrist, Graham. “Dynamics of Telomeres and MicroRNAs During Oocyte Maturation and Embryo Development in the Cow.” 2015. Web. 22 Jan 2021.

Vancouver:

Gilchrist G. Dynamics of Telomeres and MicroRNAs During Oocyte Maturation and Embryo Development in the Cow. [Internet] [Doctoral dissertation]. University of Guelph; 2015. [cited 2021 Jan 22]. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/9068.

Council of Science Editors:

Gilchrist G. Dynamics of Telomeres and MicroRNAs During Oocyte Maturation and Embryo Development in the Cow. [Doctoral Dissertation]. University of Guelph; 2015. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/9068

Louisiana State University

3. Gutierrez-Castillo, Emilio Jose. Effect of Vitrification on Epigenetic Modifications and the Meiotic Spindle of Bovine Oocytes.

Degree: MS, Animal Sciences, 2018, Louisiana State University

URL: https://digitalcommons.lsu.edu/gradschool_theses/4673

► While oocyte vitrification has become a common practice, it still faces some challenges such as the low survival rates after warming, probably related to… (more)

▼ While oocyte vitrification has become a common practice, it still faces some challenges such as the low survival rates after warming, probably related to cryoinjuries and cryoprotectant (CPA) toxicity. Evidence suggests that vitrification might have an effect on the patterns of some epigenetic marks including DNA methylation and histone acetylation. During fertilization and embryogenesis, key events for healthy and adequate embryo development take place, not only governed by the information contained within the DNA sequence, but also by epigenetic mechanisms. This study was aimed at determining the effect of vitrification and CPA exposure, using a combination of ethylene glycol (EG) with either DMSO or glycerol (Gly), on DNA methylation and histone acetylation of bovine oocytes. Additionally, the effect of vitrification and cryoprotective solutions on the meiotic spindle was evaluated. To achieve this goal, three experiments were carried out. The first experiment was intended to evaluate the effect of vitrification on DNA methylation of bovine cumulus-oocyte complexes (COCs) at two different maturation stages, germinal vesicle (GV) and metaphase II (MII). The second was designed to determine the effect of CPA exposure on DNA methylation and histone acetylation. The last experiment assessed the impact of vitrification and CPA exposure on microtubule distribution and chromosome arrangement, and if a subsequent incubation period after vitrification could promote the reorganization of the spindle. Results obtained suggest that vitrification of bovine oocytes at the GV stage does not have an effect on DNA methylation patterns. Similar outcomes were obtained when comparing oocytes in the MII vitrified with DMSO and fresh oocytes. However, oocytes vitrified with Gly showed an abnormality presented in the form of DNA fragmentation. On the other hand, exposure to EG + DMSO increases the levels of DNA methylations in comparison with fresh oocytes. CPA exposure does not have an effect on histone acetylation levels. Finally, results of the third experiment indicate that CPA exposure has no impact on the incidence of abnormal meiotic spindles. In contrast, vitrification using DMSO increases the occurrence of abnormal meiotic spindles and the damage seems to be irreversible. The incubation period following vitrification with EG + Gly promotes the reorganization of microtubules.

Subjects/Keywords: Bovine oocyte; Epigenetic modifications; Meiotic spindle

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APA (6^th Edition):

Gutierrez-Castillo, E. J. (2018). Effect of Vitrification on Epigenetic Modifications and the Meiotic Spindle of Bovine Oocytes. (Masters Thesis). Louisiana State University. Retrieved from https://digitalcommons.lsu.edu/gradschool_theses/4673

Chicago Manual of Style (16^th Edition):

Gutierrez-Castillo, Emilio Jose. “Effect of Vitrification on Epigenetic Modifications and the Meiotic Spindle of Bovine Oocytes.” 2018. Masters Thesis, Louisiana State University. Accessed January 22, 2021. https://digitalcommons.lsu.edu/gradschool_theses/4673.

MLA Handbook (7^th Edition):

Gutierrez-Castillo, Emilio Jose. “Effect of Vitrification on Epigenetic Modifications and the Meiotic Spindle of Bovine Oocytes.” 2018. Web. 22 Jan 2021.

Vancouver:

Gutierrez-Castillo EJ. Effect of Vitrification on Epigenetic Modifications and the Meiotic Spindle of Bovine Oocytes. [Internet] [Masters thesis]. Louisiana State University; 2018. [cited 2021 Jan 22]. Available from: https://digitalcommons.lsu.edu/gradschool_theses/4673.

Council of Science Editors:

Gutierrez-Castillo EJ. Effect of Vitrification on Epigenetic Modifications and the Meiotic Spindle of Bovine Oocytes. [Masters Thesis]. Louisiana State University; 2018. Available from: https://digitalcommons.lsu.edu/gradschool_theses/4673

4. Ferreira, Fernanda Altieri. Alimentação de novilhas com uréia por curto prazo afeta a qualidade de complexos cumulus oócito e o desenvolvimento de embriões In vitro.

Degree: PhD, Reprodução Animal, 2007, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/10/10131/tde-28092007-144519/ ;

►

A utilização de uréia na alimentação de fêmeas bovinas pode prejudicar o desempenho reprodutivo destes animais, provavelmente decorrente do aumento do teor de nitrogênio uréico… (more)

▼

A utilização de uréia na alimentação de fêmeas bovinas pode prejudicar o desempenho reprodutivo destes animais, provavelmente decorrente do aumento do teor de nitrogênio uréico plasmático (NUP). O objetivo deste estudo foi avaliar se alimentação com uréia por curto prazo oferecida a novilhas, e conseqüente aumento de NUP, exerce influência sobre a quantidade, qualidade e competência dos complexos cumulus-oócito (CCO). O experimento teve duração de 62 dias, dividido em oito semanas e dois períodos. Foram utilizadas 40 novilhas mestiças mantidas a pasto, alocadas a dois tratamentos, utilizando-se delineamento \"cross-over\": dieta sem uréia (SU): 5 kg (matéria original) de silagem de milho e 1 kg de concentrado/animal/dia ou dieta com uréia (U): 5 kg de silagem de milho e 1 kg de concentrado contendo 75 g de uréia/animal/dia. Os animais selecionados a cada semana receberam as dietas por seis dias, uma única vez ao dia. No sexto dia de oferecimento das dietas, foram realizadas aspiração folicular (OPU) e colheita de sangue, em jejum e 3 horas após a alimentação. Imediatamente após a OPU, foi realizada contagem total de CCO recuperados e classificação dos mesmos em viáveis e inviáveis. Apenas os viáveis foram destinados à produção In vitro de embriões. Em relação ao teor de NUP, houve efeito de interação entre tratamento e tempo de colheita (P=0,04) e dentro de cada tempo foi observado aumento significativo (P<0,01) para os animais do tratamento U. Não foi observado efeito de tratamento em relação ao número total de CCO recuperados por animal (média ± EPM: SU=9,15 ± 0,82 vs. U=8,82 ± 0,95), nem sobre a porcentagem de CCO viáveis sobre total de CCO recuperados por animal (SU=73,61% ± 2,47 vs. U=70,26% ± 2,31). A taxa de clivagem obtida no dia 3 após a inseminação In vitro (IIV) e as taxas de blastocisto nos dias 6, 7 e 9 após a IIV, não foram influenciadas pelo tratamento. Porém, no 11º pós IIV, houve diminuição (P=0,04) da taxa de blastocistos eclodidos para o tratamento U (SU=82,50% ± 0,05 vs. U=64,33% ± 0,07). Apesar do aumento do teor de NUP observado nas novilhas do tratamento U, a quantidade, qualidade e competência dos CCO durante as primeiras clivagens até atingirem o estádio de blastocisto In vitro não foram influenciadas pelo oferecimento de 75 g de uréia na dieta, durante seis dias. Porém, foi observada redução da taxa de eclosão dos blastocistos das novilhas do tratamento U.

Urea feeding offered to bovine dams may damage their reproductive performance, probably due to an increase in levels of plasmatic urea nitrogen (PUN). The aim of this study was evaluate if short-term urea feeding of heifers, following high PUN levels, would have an influence on the quantity, quality and competence of cumulus-oocyte complexes (COC). Trial lasted 62 days, divided into eight weeks and two periods. Forty crossbred grazing heifers were allocated to one of two treatments, using a cross-over design: diet without urea (WU): 5 kg (as fed) of corn silage and 1 kg of concentrated/animal/day, or diet with urea (U): 5 kg of corn…

Advisors/Committee Members: Binelli, Mario.

Subjects/Keywords: Bovine (female); Bovinos (fêmeas); Embrião; Embryo; Oócito; Oocyte; Urea; Uréia

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ferreira, F. A. (2007). Alimentação de novilhas com uréia por curto prazo afeta a qualidade de complexos cumulus oócito e o desenvolvimento de embriões In vitro. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/10/10131/tde-28092007-144519/ ;

Chicago Manual of Style (16^th Edition):

Ferreira, Fernanda Altieri. “Alimentação de novilhas com uréia por curto prazo afeta a qualidade de complexos cumulus oócito e o desenvolvimento de embriões In vitro.” 2007. Doctoral Dissertation, University of São Paulo. Accessed January 22, 2021. http://www.teses.usp.br/teses/disponiveis/10/10131/tde-28092007-144519/ ;.

MLA Handbook (7^th Edition):

Ferreira, Fernanda Altieri. “Alimentação de novilhas com uréia por curto prazo afeta a qualidade de complexos cumulus oócito e o desenvolvimento de embriões In vitro.” 2007. Web. 22 Jan 2021.

Vancouver:

Ferreira FA. Alimentação de novilhas com uréia por curto prazo afeta a qualidade de complexos cumulus oócito e o desenvolvimento de embriões In vitro. [Internet] [Doctoral dissertation]. University of São Paulo; 2007. [cited 2021 Jan 22]. Available from: http://www.teses.usp.br/teses/disponiveis/10/10131/tde-28092007-144519/ ;.

Council of Science Editors:

Ferreira FA. Alimentação de novilhas com uréia por curto prazo afeta a qualidade de complexos cumulus oócito e o desenvolvimento de embriões In vitro. [Doctoral Dissertation]. University of São Paulo; 2007. Available from: http://www.teses.usp.br/teses/disponiveis/10/10131/tde-28092007-144519/ ;

5. Sanches, Lorena [UNESP]. Efeitos do fator de crescimento dos fibroblastos 8 (fgf8) na maturação in vitro de complexos cumulus-oócito bovinos.

Degree: 2016, Universidade Estadual Paulista

URL: http://hdl.handle.net/11449/143787

► A maturação in vitro (MIV) é uma etapa fundamental da produção in vitro de embriões bovinos (PIV). A conclusão prematura da maturação nuclear durante a… (more)

Subjects/Keywords: Complexo cumulus-oócito; FGF8; Bovino; Oocyte cumulus complex; Bovine

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APA (6^th Edition):

Sanches, L. [. (2016). Efeitos do fator de crescimento dos fibroblastos 8 (fgf8) na maturação in vitro de complexos cumulus-oócito bovinos. (Thesis). Universidade Estadual Paulista. Retrieved from http://hdl.handle.net/11449/143787

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Sanches, Lorena [UNESP]. “Efeitos do fator de crescimento dos fibroblastos 8 (fgf8) na maturação in vitro de complexos cumulus-oócito bovinos.” 2016. Thesis, Universidade Estadual Paulista. Accessed January 22, 2021. http://hdl.handle.net/11449/143787.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Sanches, Lorena [UNESP]. “Efeitos do fator de crescimento dos fibroblastos 8 (fgf8) na maturação in vitro de complexos cumulus-oócito bovinos.” 2016. Web. 22 Jan 2021.

Vancouver:

Sanches L[. Efeitos do fator de crescimento dos fibroblastos 8 (fgf8) na maturação in vitro de complexos cumulus-oócito bovinos. [Internet] [Thesis]. Universidade Estadual Paulista; 2016. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/11449/143787.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Sanches L[. Efeitos do fator de crescimento dos fibroblastos 8 (fgf8) na maturação in vitro de complexos cumulus-oócito bovinos. [Thesis]. Universidade Estadual Paulista; 2016. Available from: http://hdl.handle.net/11449/143787

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan

6. Alfoteisy, Bilal. Natural honey as a cryoprotectant to improve viability of vitrified bovine oocytes.

Degree: 2012, University of Saskatchewan

URL: http://hdl.handle.net/10388/ETD-2012-01-336

► The main objective of this study was to investigate if natural honey can be used as a cryoprotecting agent (CP) in vitrification medium to improve… (more)

▼ The main objective of this study was to investigate if natural honey can be used as a cryoprotecting agent (CP) in vitrification medium to improve the viability of vitrified-warmed bovine oocytes. The first study was conducted to investigate the dehydration capability of natural honey compared with sucrose, and to determine the proper concentration of honey-based medium and the optimum time for sufficiently safe dehydration of bovine oocytes. Matured cumulus-oocyte complexs (COCs) were denuded and introduced individually into different concentrations (0.25, 0.5, 1.0, 1.5 or 2.0 M) of honey and sucrose-based medium followed by rehydration in control media (TCM). Video images were recorded during dehydration and rehydration, and oocyte images were captured at 12 time intervals to calculate oocyte-volume changes during dehydration and rehydration. Results demonstrated that, in honey-based media, the maximum oocyte shrinkage was achieved after 60 sec exposure in 0.25M, 0.5M and 1.0M concentrations; while at higher concentrations 1.5M and 2.0M, the maximum dehydration occurred at 30 and 20 seconds respectively. In sucrose-based medium, the maximum oocyte shrinkage was achieved after 60 sec exposure in 0.25 or 0.5M concentrations. However, at higher concentrations (1M, 1.5M or 2M), the maximum dehydration occurred at 30, 20 and 10 sec. For rehydration, oocytes dehydrated in honey or sucrose-based medium were able to regain their original volume within 60-120 sec. However, oocytes dehydrated in higher concentrations (2M honey, and 1.5M and 2M sucrose) were rehydrated back to their original volume within 20 sec. This study concluded that natural honey and sucrose caused similar cell dehydration. Only oocytes dehydrated in 1M honey-based media reached maximal dehydration after 60 sec and equally regained original volume. Therefore, 1M of honey-based medium is suggested for sufficient and safe oocyte dehydration during vitrification. The second study was conducted to determine in vitro maturation (IVM), in vitro fertilization (IVF) and embryonic development of bovine oocytes vitrified in honey-based vitrifcation media. In Experiment 1, bovine COCs were randomly distributed in control group (non-vitrified; G1), 0.5M sucrose group (second control; G2), and 0.5M, 1M and 1.5M honey groups (G3, G4 and G5 respectively). The COCs were exposed to equilibration solution 1 (VS1) at ~ 22 °C for 5 min and to vitrification solution 2 (VS2) for 1 min, mounted on Cryotops and plunged into LN2. COCs were warmed in TCM and honey/sucrose medium at 38.5 °C for 1 min, washed, matured in vitro (IVM), denuded, and immunostained to evaluate maturation. Maturation rate was significantly higher (80.7%) in control group (G1) than in vitrified groups (56, 52, 55 and 51% in G2, G3, G4 and G5, respectively) (P<.0001), whereas there was no significant difference among the vitrified groups (P>0.05). In Experiment 2, bovine COCs distributed in control (not vitrified, G1) and vitrified groups using 1M honey and 0.5M sucrose (G2 and G3 respectively),… Advisors/Committee Members: Muir, Gillian, Anzar, Muhammad, Lessard, Carl, Singh, Jaswant, Pettitt, Murray.

Subjects/Keywords: Vitrification medium; bovine oocyte; natural honey; volume changes and viability

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APA (6^th Edition):

Alfoteisy, B. (2012). Natural honey as a cryoprotectant to improve viability of vitrified bovine oocytes. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/ETD-2012-01-336

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Alfoteisy, Bilal. “Natural honey as a cryoprotectant to improve viability of vitrified bovine oocytes.” 2012. Thesis, University of Saskatchewan. Accessed January 22, 2021. http://hdl.handle.net/10388/ETD-2012-01-336.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Alfoteisy, Bilal. “Natural honey as a cryoprotectant to improve viability of vitrified bovine oocytes.” 2012. Web. 22 Jan 2021.

Vancouver:

Alfoteisy B. Natural honey as a cryoprotectant to improve viability of vitrified bovine oocytes. [Internet] [Thesis]. University of Saskatchewan; 2012. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/10388/ETD-2012-01-336.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Alfoteisy B. Natural honey as a cryoprotectant to improve viability of vitrified bovine oocytes. [Thesis]. University of Saskatchewan; 2012. Available from: http://hdl.handle.net/10388/ETD-2012-01-336

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

7. Prentice, Jennifer Rae. Vitrification of bovine oocytes.

Degree: 2010, University of Saskatchewan

URL: http://hdl.handle.net/10388/etd-12082010-190458

► The overall objective of this thesis was to investigate the vitrification of bovine cumulus oocyte complexes (COCs) as a tool for conservation of female genetics.… (more)

Subjects/Keywords: Bovine; Vitrification; Oocyte

…bovine cumulus oocyte complexes by in vitro maturation, fertilization and culture. 3 CHAPTER… …LENGTH OF EXPOSURE TO CRYOPROTECTANT AND WARMING SOLUTIONS, AND VITRIFICATION OF BOVINE CUMULUS… …OOCYTE COMPLEXES ON CLEAVAGE AND SUBSEQUENT EMBRYO DEVELOPMENT 81 5.1 Abstract 5.2… …thawed semen 11 Table 2.3 Differences of animal oocyte and embryo cryopreservation resistance… …among species, developmental stages and origin 14 Table 2.4 Oocyte and embryo…

10 more images…

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Prentice, J. R. (2010). Vitrification of bovine oocytes. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/etd-12082010-190458

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Prentice, Jennifer Rae. “Vitrification of bovine oocytes.” 2010. Thesis, University of Saskatchewan. Accessed January 22, 2021. http://hdl.handle.net/10388/etd-12082010-190458.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Prentice, Jennifer Rae. “Vitrification of bovine oocytes.” 2010. Web. 22 Jan 2021.

Vancouver:

Prentice JR. Vitrification of bovine oocytes. [Internet] [Thesis]. University of Saskatchewan; 2010. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/10388/etd-12082010-190458.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Prentice JR. Vitrification of bovine oocytes. [Thesis]. University of Saskatchewan; 2010. Available from: http://hdl.handle.net/10388/etd-12082010-190458

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

North Carolina State University

8. Rodriguez, Karina Flores. Molecular Mechanisms of Gonadotropin-Induced Oocyte Maturation.

Degree: PhD, Physiology, 2004, North Carolina State University

URL: http://www.lib.ncsu.edu/resolver/1840.16/3673

► In vitro maturation of oocytes is routinely utilized for the production of embryos for commercial as well as research purposes. The objectives of the research… (more)

▼ In vitro maturation of oocytes is routinely utilized for the production of embryos for commercial as well as research purposes. The objectives of the research described in this dissertation were: 1) to examine the molecular mechanism involved in gonadotropin-induced resumption of meiosis in cultured bovine and murine cumulus oocyte complexes (COC); 2) to determine the developmental potential of bovine oocytes maintained in meiotic arrest by inhibition of transcription; and 3) to analyze patterns of gene expression in bovine COC during the onset of gonadotropin-induced oocyte maturation. Murine COC were maintained in meiotic arrest by culture in the presence of either of the transcriptional inhibitors, 5,6-dichloro-1-β-D-ribofuranosylbenzimidazole (DRB) or α-amanitin. For either transcriptional inhibitor to effectively block maturation, FSH but not hCG, was required in the culture medium. Transcriptional inhibition of oocyte maturation was ineffective if denuded oocytes were utilized. Differential activation of Type I and Type II PKA was performed in murine COC. Activation of Type I PKA resulted in the inhibition of maturation whereas activation of Type II did not. Activation of Type II PKA resulted in a transcriptional event required for maturation of murine and bovine COC and mimicked the action of FSH. The developmental competency of bovine COC maintained in meiotic arrest for 20 h by DRB was not different from control COC. Comparison of the patterns of mRNA for oocytes matured for 0h, 4h or 4h+DRB resulted in the isolation of 4 amplicons that were expressed at 4h but not in 0h or 4h+DRB groups. This result was reconfirmed by semi-quantitative PCR. No homology to known sequences was found suggesting that they may represent novel transcripts. The following model for FSH induced oocyte maturation is proposed: FSH binds to its cumulus cell receptor and increases cAMP. The elevation of cAMP results in activation of Type I and Type II PKA. Activation of Type I PKA inhibits oocyte maturation whereas activation of Type II PKA induces gene transcription that subsequently leads to the resumption of meiosis. Advisors/Committee Members: Charlotte E. Farin, Committee Chair (advisor).

Subjects/Keywords: oocyte; transcription; gonadotropins; bovine; murine

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Rodriguez, K. F. (2004). Molecular Mechanisms of Gonadotropin-Induced Oocyte Maturation. (Doctoral Dissertation). North Carolina State University. Retrieved from http://www.lib.ncsu.edu/resolver/1840.16/3673

Chicago Manual of Style (16^th Edition):

Rodriguez, Karina Flores. “Molecular Mechanisms of Gonadotropin-Induced Oocyte Maturation.” 2004. Doctoral Dissertation, North Carolina State University. Accessed January 22, 2021. http://www.lib.ncsu.edu/resolver/1840.16/3673.

MLA Handbook (7^th Edition):

Rodriguez, Karina Flores. “Molecular Mechanisms of Gonadotropin-Induced Oocyte Maturation.” 2004. Web. 22 Jan 2021.

Vancouver:

Rodriguez KF. Molecular Mechanisms of Gonadotropin-Induced Oocyte Maturation. [Internet] [Doctoral dissertation]. North Carolina State University; 2004. [cited 2021 Jan 22]. Available from: http://www.lib.ncsu.edu/resolver/1840.16/3673.

Council of Science Editors:

Rodriguez KF. Molecular Mechanisms of Gonadotropin-Induced Oocyte Maturation. [Doctoral Dissertation]. North Carolina State University; 2004. Available from: http://www.lib.ncsu.edu/resolver/1840.16/3673

University of Tennessee – Knoxville

9. Hooper, Leah Marie. Impact of Heat Stress on Germinal Vesicle Breakdown and Lipolytic Changes during In Vitro Maturation of Bovine Oocytes.

Degree: MS, Animal Science, 2014, University of Tennessee – Knoxville

URL: https://trace.tennessee.edu/utk_gradthes/3158

► The main objective of this research was to examine the lipolytic changes in triglyceride and phospholipid as well as the incidence of germinal vesicle… (more)

Subjects/Keywords: Oocyte; In Vitro Maturation; Heat Stress; Lipid Metabolism; Bovine; Animal Sciences

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hooper, L. M. (2014). Impact of Heat Stress on Germinal Vesicle Breakdown and Lipolytic Changes during In Vitro Maturation of Bovine Oocytes. (Thesis). University of Tennessee – Knoxville. Retrieved from https://trace.tennessee.edu/utk_gradthes/3158

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Hooper, Leah Marie. “Impact of Heat Stress on Germinal Vesicle Breakdown and Lipolytic Changes during In Vitro Maturation of Bovine Oocytes.” 2014. Thesis, University of Tennessee – Knoxville. Accessed January 22, 2021. https://trace.tennessee.edu/utk_gradthes/3158.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Hooper, Leah Marie. “Impact of Heat Stress on Germinal Vesicle Breakdown and Lipolytic Changes during In Vitro Maturation of Bovine Oocytes.” 2014. Web. 22 Jan 2021.

Vancouver:

Hooper LM. Impact of Heat Stress on Germinal Vesicle Breakdown and Lipolytic Changes during In Vitro Maturation of Bovine Oocytes. [Internet] [Thesis]. University of Tennessee – Knoxville; 2014. [cited 2021 Jan 22]. Available from: https://trace.tennessee.edu/utk_gradthes/3158.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hooper LM. Impact of Heat Stress on Germinal Vesicle Breakdown and Lipolytic Changes during In Vitro Maturation of Bovine Oocytes. [Thesis]. University of Tennessee – Knoxville; 2014. Available from: https://trace.tennessee.edu/utk_gradthes/3158

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

North Carolina State University

10. Hockney, Jessica Eileen. Candidate mRNAs Regulating Meiotic Resumption in Bovine Cumulus-Oocyte-Complexes.

Degree: MS, Animal Science, 2008, North Carolina State University

URL: http://www.lib.ncsu.edu/resolver/1840.16/2490

► In bovine oocytes, the resumption of meiosis is characterized by the breakdown of the germinal vesicle (GVBD). When cumulus-oocyte complexes (COCs) are cultured in-vitro in… (more)

▼ In bovine oocytes, the resumption of meiosis is characterized by the breakdown of the germinal vesicle (GVBD). When cumulus-oocyte complexes (COCs) are cultured in-vitro in the presence of gonadotropins, GVBD is characterized by an initial inhibitory phase, which is followed by an acceleration in the rate of GVBD. An initial transcriptional event is required for gonadotropin-induced in-vitro maturation. The objectives of this thesis were: 1) to define the time course required for transcriptional initiation in bovine cumulus oocyte complexes (COCs); 2) to determine the pattern of expression for Nr4A1 and Egr1 mRNAs in bovine COCs; and 3) to reduce the expression of Nr4A1 mRNA expression to determine its effect on oocyte maturation. Bovine COCs were cultured in the presence follicle stimulating hormone (FSH) alone or FSH with the transcriptional inhibitor, 5,6-dichloro-1-B-Dribofuranosylbenzamidazole (DRB), in order to refine the time course required for transcription initiation and to determine the pattern of expression for Nr4A1 and Egr1 mRNAs. All experiments contained a control group of COCs that were cultured for the entire duration in the presence of DRB. By adding DRB at 0, 30, 60, 90, 120, 150, or 180 minutes after the initiation of culture, it was determined that gene transcription required for GVBD occurs between 0 and 60 minutes after the start of culture. Analysis of COCs cultured for 0, 30, 60, 90 or 180 minutes demonstrated that Nr4A1 mRNA levels increased significantly (P<0.05) at 30 minutes after the start of culture, which is consistent with the time of transcription initiation required for GVBD. In contrast, Egr1 mRNA levels did not significantly differ throughout the culture period. Small interfering RNAs (siRNAs) designed from the sequence for Nr4A1 were used to reduce Nr4A1 mRNA expression and determine the effects of Nr4A1 mRNAs on GVBD in bovine COCs. Expression of Nr4A1 mRNA decreased in abundance in treatment groups containing siRNAs specific to Nr4A1 (siNr4A1) with the greatest decrease in expression occurring in the 25nM and 50nM siNr4A1 treatments. As expected, fewer COCs underwent GVBD when cultured in the presence of DRB at 9 and 20 hours as compared to COCs cultured in FSH alone. Additionally, no significant differences were observed between the FSH and nonspecific siRNA (siNS) treatment groups in the proportion of COCs undergoing GVBD at either 9 or 20 hours of culture. Fewer COCs cultured in the presence of 50nM siNr4A1 underwent GVBD by 9 hours of culture as compared to those cultured in FSH alone. The percentage of COCs that underwent GVBD did not differ between the siNr4A1 and FSH control treatments at 20 hours. The expression of Nr4A1 mRNA at 30 minutes after the start of culture did not differ with FSH, siNr4A1, or siNS treatments. In summary, gene transcription required for GVBD in bovine COCs occurs within 0 to 60 minutes of culture. Nr4A1 mRNAs are present in bovine COCs and these mRNA levels increase significantly after 30 minutes of culture. Furthermore, Egr1 mRNAs are present… Advisors/Committee Members: Dr. William L. Miller, Committee Member (advisor), Dr. Charlotte E. Farin, Committee Chair (advisor), Dr. Robert M. Petters, Committee Member (advisor).

Subjects/Keywords: COC; Egr1; Nr4a1; in vitro; oocyte maturation; cow; bovine; cumulus oocyte complex; oocyte

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hockney, J. E. (2008). Candidate mRNAs Regulating Meiotic Resumption in Bovine Cumulus-Oocyte-Complexes. (Thesis). North Carolina State University. Retrieved from http://www.lib.ncsu.edu/resolver/1840.16/2490

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Hockney, Jessica Eileen. “Candidate mRNAs Regulating Meiotic Resumption in Bovine Cumulus-Oocyte-Complexes.” 2008. Thesis, North Carolina State University. Accessed January 22, 2021. http://www.lib.ncsu.edu/resolver/1840.16/2490.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Hockney, Jessica Eileen. “Candidate mRNAs Regulating Meiotic Resumption in Bovine Cumulus-Oocyte-Complexes.” 2008. Web. 22 Jan 2021.

Vancouver:

Hockney JE. Candidate mRNAs Regulating Meiotic Resumption in Bovine Cumulus-Oocyte-Complexes. [Internet] [Thesis]. North Carolina State University; 2008. [cited 2021 Jan 22]. Available from: http://www.lib.ncsu.edu/resolver/1840.16/2490.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hockney JE. Candidate mRNAs Regulating Meiotic Resumption in Bovine Cumulus-Oocyte-Complexes. [Thesis]. North Carolina State University; 2008. Available from: http://www.lib.ncsu.edu/resolver/1840.16/2490

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

11. Tatimara Maria Miyauchi. Protocolos hormonais de preparação de doadoras bovinas para produção de embriões in vitro.

Degree: 2011, Universidade Jose do Rosario Vellano

URL: http://tede.unifenas.br/tde_busca/arquivo.php?codArquivo=62

►

Efficiency of in vitro bovine embryo production has been affected by low quantity and quality of recovered oocytes from ultrasound-guided follicular aspiration (OPU). The objective… (more)

▼

Efficiency of in vitro bovine embryo production has been affected by low quantity and quality of recovered oocytes from ultrasound-guided follicular aspiration (OPU). The objective of this study was to evaluate the effect of different synchronization of follicular wave protocols in the production of oocytes and embryos in vitro, of Bos taurus taurus and Bos taurus indicus and heifers. We used three hundred forty-four results of follicular aspiration. The management regime was semi- confinement of animals with positive energy balance ten days prior to the aspirations. Animals were randomly distributed in the following treatments: TI or control (N=81) - OPU independent of estrous cycle with no previous hormone treatment; TII (N=97) - Estradiol benzoate (3mg), four days before OPU; TIII (N= 97) - Estradiol benzoate (3mg), insertion of subcutaneous implant of Norgestomed and application of D-Chloprostenol Sodium (0.150 mg), four days before OPU; TIV (N= 69) the same treatment used in TIII, plus two doses of FSH (100 to 150 UI) one day before OPU, with an interval of 12 hours, OPU after 12 to 16 hours. Viable recovered oocytes were in vitro matured, fertilized and cultured and presumptive zygotes were cultured until the time of embryo transfer. Zebu showed greater recovery of viable oocytes and total production of embryos in relation to taurine respectively (14.2 0.7 vs 8.8 0.5; 17.7 0.9 vs 11.9 0.6; 4.3 0.3 vs 2.1 0.2). A significant increase in quality and oocyte recovery in groups III and IV in relation to control group, respectively (13.2 1.0 vs 13.8 1.1 vs 8.1 0.8; 16.8 1.2 vs 17.8 1.4 vs 10.7 1.0 , P <0.05). In relation to the total quantity and quality of embryos, all treatments were superior to control (P <0.05), but T IV was superior to other treatments according to the breeds studied. Treatments III and IV showed a greater recovered oocyte number in Bos taurus; the same was not observed in Zebu. A better average conversion was observed in Zebu cows. Bos indicus presented greater quality and recover rate of oocytes in summer compared to winter (15.7 1.6 vs 12.0 1.0; 19.3 1.3 vs 15.3 1.2). Bos indicus produced more embryos in both seasons and, in winter, both subspecies converted more oocytes into embryos compared with to summer (41.5 3.5 vs 33.1 2.4; 28.5 3.2 vs 19.5 2.6). Synchronization treatments were efficient in oocyte recovering of Bos taurus donors and Bos indicus embryos. Regarding the season of the year, winter has proved to be the most favorable period for conversion of embryos for the two subspecies.

A eficiência na produção in vitro de embriões bovinos é limitada pela quantidade e qualidade dos oócitos recuperados na aspiração folicular guiada por ultrassonografia (OPU). Objetivou-se com o presente estudo avaliar os efeitos de diferentes protocolos de sincronização da onda folicular na recuperação de oócitos e produção de embriões bovinos in vitro, das subespécies Bos taurus taurus e Bos taurus indicus em diferentes épocas do ano. Foram utilizados trezentos e quarenta e quatro resultados de…

Advisors/Committee Members: Marilu Martins Gioso, João Henrique Moreira Viana, Carlos Antônio de Carvalho Fernandes.

Subjects/Keywords: bovinos; fêmeas; hormônios; aspiração folicular (OPU); REPRODUCAO ANIMAL; oocyte; hormone; oócitos; OPU; female; bovine

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❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Miyauchi, T. M. (2011). Protocolos hormonais de preparação de doadoras bovinas para produção de embriões in vitro. (Thesis). Universidade Jose do Rosario Vellano. Retrieved from http://tede.unifenas.br/tde_busca/arquivo.php?codArquivo=62

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Miyauchi, Tatimara Maria. “Protocolos hormonais de preparação de doadoras bovinas para produção de embriões in vitro.” 2011. Thesis, Universidade Jose do Rosario Vellano. Accessed January 22, 2021. http://tede.unifenas.br/tde_busca/arquivo.php?codArquivo=62.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Miyauchi, Tatimara Maria. “Protocolos hormonais de preparação de doadoras bovinas para produção de embriões in vitro.” 2011. Web. 22 Jan 2021.

Vancouver:

Miyauchi TM. Protocolos hormonais de preparação de doadoras bovinas para produção de embriões in vitro. [Internet] [Thesis]. Universidade Jose do Rosario Vellano; 2011. [cited 2021 Jan 22]. Available from: http://tede.unifenas.br/tde_busca/arquivo.php?codArquivo=62.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Miyauchi TM. Protocolos hormonais de preparação de doadoras bovinas para produção de embriões in vitro. [Thesis]. Universidade Jose do Rosario Vellano; 2011. Available from: http://tede.unifenas.br/tde_busca/arquivo.php?codArquivo=62

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

12. Tatimara Maria Miyauchi. Different protocols to bovine donor preparation for in vitro embryo production.

Degree: 2011, Universidade Jose do Rosario Vellano

URL: http://tede.unifenas.br/tde_busca/arquivo.php?codArquivo=74

►

Efficiency of in vitro bovine embryo production has been affected by low quantity and quality of recovered oocytes from ultrasound-guided follicular aspiration (OPU). The objective… (more)

▼

Efficiency of in vitro bovine embryo production has been affected by low quantity and quality of recovered oocytes from ultrasound-guided follicular aspiration (OPU). The objective of this study was to evaluate the effect of different synchronization of follicular wave protocols in the production of oocytes and embryos in vitro, of Bos taurus taurus and Bos taurus indicus and heifers. We used three hundred forty-four results of follicular aspiration. The management regime was semi- confinement of animals with positive energy balance ten days prior to the aspirations. Animals were randomly distributed in the following treatments: TI or control (N=81) - OPU independent of estrous cycle with no previous hormone treatment; TII (N=97) - Estradiol benzoate (3mg), four days before OPU; TIII (N= 97) - Estradiol benzoate (3mg), insertion of subcutaneous implant of Norgestomed and application of D-Chloprostenol Sodium (0.150 mg), four days before OPU; TIV (N= 69) the same treatment used in TIII, plus two doses of FSH (100 to 150 UI) one day before OPU, with an interval of 12 hours, OPU after 12 to 16 hours. Viable recovered oocytes were in vitro matured, fertilized and cultured and presumptive zygotes were cultured until the time of embryo transfer. Zebu showed greater recovery of viable oocytes and total production of embryos in relation to taurine respectively (14.2 0.7 vs 8.8 0.5; 17.7 0.9 vs 11.9 0.6; 4.3 0.3 vs 2.1 0.2). A significant increase in quality and oocyte recovery in groups III and IV in relation to control group, respectively (13.2 1.0 vs 13.8 1.1 vs 8.1 0.8; 16.8 1.2 vs 17.8 1.4 vs 10.7 1.0 , P <0.05). In relation to the total quantity and quality of embryos, all treatments were superior to control (P <0.05), but T IV was superior to other treatments according to the breeds studied. Treatments III and IV showed a greater recovered oocyte number in Bos taurus; the same was not observed in Zebu. A better average conversion was observed in Zebu cows. Bos indicus presented greater quality and recover rate of oocytes in summer compared to winter (15.7 1.6 vs 12.0 1.0; 19.3 1.3 vs 15.3 1.2). Bos indicus produced more embryos in both seasons and, in winter, both subspecies converted more oocytes into embryos compared with to summer (41.5 3.5 vs 33.1 2.4; 28.5 3.2 vs 19.5 2.6). Synchronization treatments were efficient in oocyte recovering of Bos taurus donors and Bos indicus embryos. Regarding the season of the year, winter has proved to be the most favorable period for conversion of embryos for the two subspecies.

A eficiência na produção in vitro de embriões bovinos é limitada pela quantidade e qualidade dos oócitos recuperados na aspiração folicular guiada por ultrassonografia (OPU). Objetivou-se com o presente estudo avaliar os efeitos de diferentes protocolos de sincronização da onda folicular na recuperação de oócitos e produção de embriões bovinos in vitro, das subespécies Bos taurus taurus e Bos taurus indicus em diferentes épocas do ano. Foram utilizados trezentos e quarenta e quatro resultados de…

Advisors/Committee Members: Carlos Antônio de Carvalho Fernandes, Marilu Martins Gioso, João Henrique Moreira Viana.

Subjects/Keywords: hormônios; female; oocyte; fêmeas; REPRODUCAO ANIMAL; bovinos; aspiração folicular (OPU); oócitos; bovine; hormone; OPU

Record Details Similar Records Cite « Share

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Miyauchi, T. M. (2011). Different protocols to bovine donor preparation for in vitro embryo production. (Thesis). Universidade Jose do Rosario Vellano. Retrieved from http://tede.unifenas.br/tde_busca/arquivo.php?codArquivo=74

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Miyauchi, Tatimara Maria. “Different protocols to bovine donor preparation for in vitro embryo production.” 2011. Thesis, Universidade Jose do Rosario Vellano. Accessed January 22, 2021. http://tede.unifenas.br/tde_busca/arquivo.php?codArquivo=74.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Miyauchi, Tatimara Maria. “Different protocols to bovine donor preparation for in vitro embryo production.” 2011. Web. 22 Jan 2021.

Vancouver:

Miyauchi TM. Different protocols to bovine donor preparation for in vitro embryo production. [Internet] [Thesis]. Universidade Jose do Rosario Vellano; 2011. [cited 2021 Jan 22]. Available from: http://tede.unifenas.br/tde_busca/arquivo.php?codArquivo=74.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Miyauchi TM. Different protocols to bovine donor preparation for in vitro embryo production. [Thesis]. Universidade Jose do Rosario Vellano; 2011. Available from: http://tede.unifenas.br/tde_busca/arquivo.php?codArquivo=74

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

13. Lemes, Rafaella Curvelano. Análise do perfil de expressão gênica de oócitos bovinos advindos de complexos cumulus-oócito com diferentes qualidades morfológicas.

Degree: Mestrado, Genética, 2013, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/17/17135/tde-29082013-163547/ ;

►

A busca por melhores resultados nas biotecnologias reprodutivas leva a um estudo dos mecanismos fisiológicos básicos dos gametas e embriões em estágios iniciais. Entender como… (more)

▼

A busca por melhores resultados nas biotecnologias reprodutivas leva a um estudo dos mecanismos fisiológicos básicos dos gametas e embriões em estágios iniciais. Entender como funciona o desenvolvimento destes, quais os nutrientes que eles precisam para se desenvolver in vitro e quais as condições ambientais necessárias permitem maiores taxas de sucesso. Tendo em vista a heterogeneidade dos complexos cumulus-oócito (COC) bovinos recuperados de ovários obtidos em frigorífico para a maturação in vitro e a relação entre oócito e células do cumulus nos resultados da produção in vitro de embriões, esse trabalho visa a identificação de fatores moleculares que possam explicar as melhores taxas de blastocistos obtidas por COCs com mais de três camadas de células do cumulus compactas e ooplasma homogêneo (COCI) quando comparados com COCs com menos de duas camadas de células do cumulus, com parte da zona pelúcida exposta e ooplasma homogêneo ou heterogêneo (COCIII). Para isso, COCI e III foram maturados in vitro separadamente, foram vortexados para retirada das células do cumulus e os oócitos foram submetidos a extração de RNA. O RNA foi amplificado em aRNA, marcado com biotina, fragmentado e hibridizado em microarray. Os arrays foram escaneados e os dados gerados analisados pelo métodos Significance Analysis of Microarrays e Rank Products em busca de genes diferencialmente expressos (DEG). A análise funcional in silico foi realizada com a ferramenta Ingenuity Pathway Analysis. Os resultados mostraram que o perfil de expressão dos oócitos de COCIII é diferente do perfil dos oócitos de COCI, como pode ser observado pelos 446 DEGs identificados, sendo 24 com expressão aumentada em oócitos de COCIII. Os genes com expressão alterada estão envolvidos em processos básicos da célula, como reassumição da meiose, metabolismo do oócito e maturação molecular, o que corrobora a ideia de que uma grande quantidade de células do cumulus interagindo com o oócito é crucial para a competência de desenvolvimento.

The researches for better results in reproductive biotechnologies have leading to studies of the basic physiological mechanisms of gametes and embryos in the early stages of developmental. Understand about their development, which nutrients they need and what environmental conditions are necessary to allow higher success rates. Because of the cumulus-oocyte complexes (COC) heterogeneous recovered from bovine ovaries obtained from an abattoir used for in vitro maturation and the relationship between oocyte and cumulus cells in the in vitro production of embryos, this study aims to identify molecular factors which might explain the improved rates of blastocyst obtained by COCs with more than three compact layers of cumulus cells and homogeneous ooplasm (COCI) compared with COC with less than two layers of cumulus cells, with part of the zona pellucida exposed and homogeneous or heterogeneous ooplasm (COCIII). For this, COCI and III were matured in vitro separately, their cumulus cells were removed and…

Advisors/Committee Members: Lôbo, Raysildo Barbosa.

Subjects/Keywords: Microarray; Bovine; Bovino; Complexo cumulus-oócito; Cumulus-oocyte complex; Microarray; Morphological quality; Qualidade morfológica

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lemes, R. C. (2013). Análise do perfil de expressão gênica de oócitos bovinos advindos de complexos cumulus-oócito com diferentes qualidades morfológicas. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/17/17135/tde-29082013-163547/ ;

Chicago Manual of Style (16^th Edition):

Lemes, Rafaella Curvelano. “Análise do perfil de expressão gênica de oócitos bovinos advindos de complexos cumulus-oócito com diferentes qualidades morfológicas.” 2013. Masters Thesis, University of São Paulo. Accessed January 22, 2021. http://www.teses.usp.br/teses/disponiveis/17/17135/tde-29082013-163547/ ;.

MLA Handbook (7^th Edition):

Lemes, Rafaella Curvelano. “Análise do perfil de expressão gênica de oócitos bovinos advindos de complexos cumulus-oócito com diferentes qualidades morfológicas.” 2013. Web. 22 Jan 2021.

Vancouver:

Lemes RC. Análise do perfil de expressão gênica de oócitos bovinos advindos de complexos cumulus-oócito com diferentes qualidades morfológicas. [Internet] [Masters thesis]. University of São Paulo; 2013. [cited 2021 Jan 22]. Available from: http://www.teses.usp.br/teses/disponiveis/17/17135/tde-29082013-163547/ ;.

Council of Science Editors:

Lemes RC. Análise do perfil de expressão gênica de oócitos bovinos advindos de complexos cumulus-oócito com diferentes qualidades morfológicas. [Masters Thesis]. University of São Paulo; 2013. Available from: http://www.teses.usp.br/teses/disponiveis/17/17135/tde-29082013-163547/ ;

14. Feitosa, Weber Beringui. Proteína quinase C (PKC) e proteína quinase dependente de cálcio/calmodulina (CaMK II) na ativação de oócitos bovinos.

Degree: PhD, Reprodução Animal, 2010, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/10/10131/tde-22092010-133245/ ;

►

A fecundação resulta no aumento intracelular de cálcio que é necessário para a transição do oócito até o estádio de zigoto. Os eventos que ocorrem… (more)

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A fecundação resulta no aumento intracelular de cálcio que é necessário para a transição do oócito até o estádio de zigoto. Os eventos que ocorrem durante esta transição são caracterizados como ativação, sendo estes dependentes de cálcio. Entretanto, os eventos bioquímicos que ocorrem durante a ativação ainda não estão completamente elucidados. A proteína quinase C (PKC) e a proteína quinase dependente de cálcio/calmodulina (CaMKII), por apresentarem atividade durante a fecundação e por serem ativadas por cálcio são implicadas na regulação dos eventos da ativação. Entretanto, existem muitas dúvidas sobre o real papel destas proteínas na ativação do oócito. Deste modo, o objetivo do presente trabalho foi avaliar o papel da PKC e da CaMKII na ativação de oócitos bovinos. Para tal, oócitos bovinos maturados in vitro foram ativados partenogeneticamente (AP) com cálcio ionóforo A23187 (5μM) por 5 minutos, sendo a retomada da meiose, a organização do citoesqueleto e do retículo endoplasmático (RE) avaliada 1 hora após a ativação. No experimento 1 foi avaliado o papel da CaMKII nestes eventos. Os oócitos foram AP na presença ou ausência de 100M do inibidor de CaMKII (Autocamtide-2 Related Inhibitory Peptide, Myristoylated). A inibição da CaMKII não afetou a retomada da meiose e nem a distribuição dos RE, após a AP. Entretanto, não ocorreu a rotação do fuso meiótico no estádio de telófase II quando a CaMKII foi inibidada. Estes resultados demonstram que embora a CaMKII não tenha efeito na retomada da meiose, esta proteína participa na progressão do ciclo celular de oócitos bovinos, após a AP. No experimento 2 foi avaliado o papel da PKC em oócitos bovinos AP. Os oócitos foram ativados partenogeneticamente na presença ou ausência de 10μM do inibidor de PKC (Bisindolymaleimide I). A inibição da PKC não afetou a retomada da meiose e nem a progressão pelo ciclo celular até o estádio de telófase II. Entretanto, a organização do RE foi afetada pela inibição da PKC. Resultado semelhante foi obtido quando os oócitos foram ativados na presença de citocalasina C, um despolimerizador de filamentos de actina. O presente experimento demonstra a participação da via PKC-actina na organização do RE na ativação de oócitos bovinos.

The intracellular calcium increase resulting from fertilization is necessary for oocyte transition to zygote. The events that occur during this transition are characterized as activation, which are dependent on calcium. However the biochemical events that occur during this activation are still not fully elucidated. The protein kinase C (PKC) and the calcium/calmodulin-dependent protein kinase II (CaMKII), are involved in regulating the events of activation, since these proteins have activity during fertilization and are activated by calcium. However there are many doubts about the real role of these proteins in the oocyte activation. Thus, the objective of this study was to evaluate the role of PKC and CaMKII in bovine oocyte activation. For this purpose, in vitro matured bovines oocytes were…

Advisors/Committee Members: Assumpção, Mayra Elena Ortiz D\'Ávila.

Subjects/Keywords: Bovine; Bovinos; CaMK II; CaMKII; Endoplasmic reticulum; Oócito; Oocyte; PKC; PKC; Retículo endoplasmático

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Feitosa, W. B. (2010). Proteína quinase C (PKC) e proteína quinase dependente de cálcio/calmodulina (CaMK II) na ativação de oócitos bovinos. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/10/10131/tde-22092010-133245/ ;

Chicago Manual of Style (16^th Edition):

Feitosa, Weber Beringui. “Proteína quinase C (PKC) e proteína quinase dependente de cálcio/calmodulina (CaMK II) na ativação de oócitos bovinos.” 2010. Doctoral Dissertation, University of São Paulo. Accessed January 22, 2021. http://www.teses.usp.br/teses/disponiveis/10/10131/tde-22092010-133245/ ;.

MLA Handbook (7^th Edition):

Feitosa, Weber Beringui. “Proteína quinase C (PKC) e proteína quinase dependente de cálcio/calmodulina (CaMK II) na ativação de oócitos bovinos.” 2010. Web. 22 Jan 2021.

Vancouver:

Feitosa WB. Proteína quinase C (PKC) e proteína quinase dependente de cálcio/calmodulina (CaMK II) na ativação de oócitos bovinos. [Internet] [Doctoral dissertation]. University of São Paulo; 2010. [cited 2021 Jan 22]. Available from: http://www.teses.usp.br/teses/disponiveis/10/10131/tde-22092010-133245/ ;.

Council of Science Editors:

Feitosa WB. Proteína quinase C (PKC) e proteína quinase dependente de cálcio/calmodulina (CaMK II) na ativação de oócitos bovinos. [Doctoral Dissertation]. University of São Paulo; 2010. Available from: http://www.teses.usp.br/teses/disponiveis/10/10131/tde-22092010-133245/ ;

15. Lopes, Everton. Ação da Proteína Kinase C na maturação de oócitos bovinos.

Degree: Mestrado, Reprodução Animal, 2012, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/10/10131/tde-19042013-145633/ ;

► A qualidade do oócito é um fator limitante na fertilidade das fêmeas e reflete seu intrínseco potencial ao desenvolvimento embrionário subsequente. As alterações moleculares e… (more)

▼ A qualidade do oócito é um fator limitante na fertilidade das fêmeas e reflete seu intrínseco potencial ao desenvolvimento embrionário subsequente. As alterações moleculares e bioquímicas no processo de maturação dos oócitos são necessárias para permitir a fecundação destes. Sob influência das gonadotrofinas, uma cascata de eventos é desencadeada, alterando a expressão gênica e a estrutura dos folículos. A maturação ocorre pelo intercâmbio entre o oócito e as células do cumulus que irão fornecer fatores para o desenvolvimento do oócito e criar o microambiente necessário para garantir o sucesso na maturação. A ação do FSH sobre a retomada da meiose ocorre, possivelmente, por ativação da proteína quinase C (PKC). A via de sinalização desta proteína parece estar envolvida na ativação da quinase ativada por mitógeno (MAPK) em oócitos e células do cumulus, na maturação induzida por FSH e LH, além de regular a síntese do Fator de Crescimento Epidermal (EGF). Deste modo, o objetivo do presente trabalho foi avaliar a ação da PKC na maturação de oócitos bovinos e se esta ativação envolve o EGF. Para tal foram realizados dois experimentos. Em ambos, a progressão do ciclo celular foi avaliada utilizando a sonda fluorescente Hoechst 33342. A expansão das células do cumulus foi avaliada utilizando-se o software Image Pro Plus 5.1 para análise das imagens dos oócitos geradas em microscópio Olympus IX81. O maior diâmetro de cada complexo cumulus oócito foi adotado como parâmetro de mensuração da expansão. A dosagem de progesterona do meio de cultivo foi realizada pela técnica de RIA. A ativação da PKC e da MAPK foi avaliada pela técnica de Western blot. Os dados foram avaliados pelo software SigmaPlot versão 12.2 e submetidos ao teste de normalidade (Shapiro-Wilk). Quando necessário, os dados foram transformados. Para comparação entre dois tratamentos, utilizou-se o teste t-student. Para mais de dois tratamentos foi realizada análise de variância e teste de comparação de médias (TUKEY), considerando-se 0,05 para rejeitar a hipótese de nulidade. No experimento 1 foi avaliado se a ativação da PKC foi estimulada por gonadotrofinas. Os oócitos foram maturados in vitro tratados com gonadotrofinas, na presença ou ausência do inibidor de PKC. A presença do inibidor de PKC diminuiu as taxas de quebra de vesícula germinativa e a expansão das células do cumulus, sem alterar a esteroidogênese. Estes resultados demonstram que a PKC participa da via de sinalização da retomada da meiose. No experimento 2 foi avaliado se o EGF está envolvido na via regulada pela PKC. Os oócitos foram maturados in vitro, na presença ou ausência de LH e FSH, do inibidor de PKC e do EGF. O EGF foi capaz de reverter os efeitos do inibidor de PKC, aumentando as taxas de quebra de vesícula germinativa e a expansão de células do cumulus. Não foi possível detectar, nas condições deste experimento, a ativação das proteínas PKC e MAPK através do Western Blot. Este trabalho permite concluir que a via de sinalização da maturação de oócitos bovinos envolve a PKC e sugere a… Advisors/Committee Members: Assumpção, Mayra Elena Ortiz D'Avila.

Subjects/Keywords: Bloqueio meiótico; Bovine oocyte; EGF; EGF; Gonadotrofinas; Gonadotropins; Meiotic arrest; Oócito bovino; PKC; PKC

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lopes, E. (2012). Ação da Proteína Kinase C na maturação de oócitos bovinos. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/10/10131/tde-19042013-145633/ ;

Chicago Manual of Style (16^th Edition):

Lopes, Everton. “Ação da Proteína Kinase C na maturação de oócitos bovinos.” 2012. Masters Thesis, University of São Paulo. Accessed January 22, 2021. http://www.teses.usp.br/teses/disponiveis/10/10131/tde-19042013-145633/ ;.

MLA Handbook (7^th Edition):

Lopes, Everton. “Ação da Proteína Kinase C na maturação de oócitos bovinos.” 2012. Web. 22 Jan 2021.

Vancouver:

Lopes E. Ação da Proteína Kinase C na maturação de oócitos bovinos. [Internet] [Masters thesis]. University of São Paulo; 2012. [cited 2021 Jan 22]. Available from: http://www.teses.usp.br/teses/disponiveis/10/10131/tde-19042013-145633/ ;.

Council of Science Editors:

Lopes E. Ação da Proteína Kinase C na maturação de oócitos bovinos. [Masters Thesis]. University of São Paulo; 2012. Available from: http://www.teses.usp.br/teses/disponiveis/10/10131/tde-19042013-145633/ ;

Universidade Federal de Viçosa

16. Vívian Rachel de Araújo Mendes. Detecção viral e capacidade de maturação in vitro de ovócitos de vacas naturalmente infectadas pelo Herpesvirus bovino 1.

Degree: 2012, Universidade Federal de Viçosa

URL: http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=5534

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O objetivo do presente estudo foi verificar a presença do DNA do herpesvirus bovino 1 (BoHV1) em Complexos Cumulus Oóforus (COCs) e no sangue, além… (more)

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O objetivo do presente estudo foi verificar a presença do DNA do herpesvirus bovino 1 (BoHV1) em Complexos Cumulus Oóforus (COCs) e no sangue, além de avaliar a capacidade de desenvolvimento de ovócitos oriundos de vacas infectadas naturalmente. Os COCs foram obtidos de 15 doadoras por meio de aspiração folicular guiada por ultrassom (OPU). A extração do DNA foi realizada em um pool de COCs de todas as aspirações de uma mesma doadora e no sangue total, para a realização das reações de Nested-PCR. Os tratamentos foram definidos a partir do título de anticorpos detectados pela soroneutralização em microplacas, sendo estabelecidos quatro grupos: animais negativos (título menor do que 2); título baixo (2 a 8); título médio (16 a 32) e título alto (64 a 512). Foram selecionados para o cultivo in vitro somente os COCs com camada compacta de células do cumulus, zona pelúcida íntegra e citoplasma homogêneo. Após 24 horas de cultivo, os ovócitos foram fixados e corados para avaliação da maturação nuclear. O estádio do ciclo celular meiótico foi avaliado, por meio de um microscópio óptico com aumento de 1000X em imersão. Foi considerado maduro o ovócito que atingiu o estádio de metáfase II. O DNA do BoHV1 foi identificado no pool de COCs de três doadoras soropositivas, não sendo detectado em nenhuma das amostras soronegativas. Entretanto, não foi encontrado DNA viral em amostras sanguíneas, mesmo nas oriundas de animais soropositivos. Não houve diferença (P>0,05) na taxa de maturação nuclear ovocitária do grupo controle negativo (76,7%), quando comparada com a observada em animais com baixa titulação de anticorpos contra o BoHV1 (62,2%). Entretanto, verificou-se menor taxa de maturação nuclear (P<0,05) em ovócitos oriundos de vacas com titulação média (48,4%) e alta (50,9%), quando comparadas com o controle negativo. Os resultados obtidos permitem concluir que vacas infectadas naturalmente pelo BoHV1 podem apresentar o DNA viral em estruturas ovarianas e ainda ter comprometimento na taxa de maturação nuclear ovocitária.

This study was carried out to verify the presence of DNA of the bovine herpesvirus 1 (BoHV1) in Cumulus Oophorus Complexes (COCs) and in blood, as well as to evaluate the development ability of the oocytes from the naturally infected cows. COCs were obtained from 15 donors through follicular aspiration guided by ultrasound (OPU). The DNA extraction was accomplished in a pool of COCs of all aspirations from a same donor and in the total blood, in order to accomplish the Nested-PCR reactions. The treatments were defined from the title of the antibodies detected by serum-neutralization in microplates, and four groups were established: negative animals (title lower than 2); low title (2 to 8); medium title (16 to 32) and high title (64 to 512). Only the COCs provided with compact layer of cumulus cells, an integral pellucid zone and homogenous cytoplasm were selected for in vitro culture. After 24 hours under culture, the oocytes were fixed and stained for evaluation of the nuclear maturation. The stage of the…

Advisors/Committee Members: Eduardo Paulino da Costa, José Domingos Guimarães, Abelardo Silva Júnior, Marcelo Rezende Luz.

Subjects/Keywords: Ovócito bovino; BoHV1; Maturação "in vitro"; REPRODUCAO ANIMAL; Bovine oocyte; BoHV1; "In vitro" maturation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mendes, V. R. d. A. (2012). Detecção viral e capacidade de maturação in vitro de ovócitos de vacas naturalmente infectadas pelo Herpesvirus bovino 1. (Thesis). Universidade Federal de Viçosa. Retrieved from http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=5534

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mendes, Vívian Rachel de Araújo. “Detecção viral e capacidade de maturação in vitro de ovócitos de vacas naturalmente infectadas pelo Herpesvirus bovino 1.” 2012. Thesis, Universidade Federal de Viçosa. Accessed January 22, 2021. http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=5534.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mendes, Vívian Rachel de Araújo. “Detecção viral e capacidade de maturação in vitro de ovócitos de vacas naturalmente infectadas pelo Herpesvirus bovino 1.” 2012. Web. 22 Jan 2021.

Vancouver:

Mendes VRdA. Detecção viral e capacidade de maturação in vitro de ovócitos de vacas naturalmente infectadas pelo Herpesvirus bovino 1. [Internet] [Thesis]. Universidade Federal de Viçosa; 2012. [cited 2021 Jan 22]. Available from: http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=5534.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mendes VRdA. Detecção viral e capacidade de maturação in vitro de ovócitos de vacas naturalmente infectadas pelo Herpesvirus bovino 1. [Thesis]. Universidade Federal de Viçosa; 2012. Available from: http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=5534

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Federal de Santa Maria

17. Luciano Ricardo Sandri. EFEITO DE BLOQUEADORES MEIÓTICOS NA MATURAÇÃO E ULTRAESTRUTURA DE OÓCITOS E SUA CONSEQÜÊNCIA NA PRODUÇÃO DE EMBRIÕES IN VITRO.

Degree: 2007, Universidade Federal de Santa Maria

URL: http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=1087

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O objetivo desse trabalho foi testar o efeito do Cordicepim (CY), um bloqueador da tradução do RNAm, na maturação citoplasmática do oócito bovino juntamente a… (more)

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O objetivo desse trabalho foi testar o efeito do Cordicepim (CY), um bloqueador da tradução do RNAm, na maturação citoplasmática do oócito bovino juntamente a um fator de crescimento (IGF-I), associado ou não ao Roscovitina (Ros), um bloqueador do fator promotor da mitose. Doze horas em cultivo com CY seguidos de 18 ou 24 horas de maturação foi suficente para que 80% dos oócitos, em média, atingissem metáfase II. A produção de blastocisto após 12 horas de cultivo em meio com CY seguido de 18 horas (32,3%) de cultivo livre de inibição não apresentou diferença significativa em relação ao grupo controle (39,6%; P>0,05). Um efeito negativo do Ros na produção de blastocistos foi observado quando não associado ao CY (16,7% e 49% de blastocistos, respectivamente). A diminuição na concentração de CY e Ros, quando associados, causou uma queda na produção de blastocisto (8,3% e 36,4% de blastocistos, respectivamente). Utilizando técnica de microscopia eletrônica de transmissão foi possível observar que o cultivo por 12 horas em CY manteve o oócito com algumas características de imaturo, porém com acúmulo e atividades de algumas organelas característico de oócito maturo. Em conclusão, a exposição temporária de oócitos bovinos ao CY associado ao IGF-I, durante a maturação in vitro, permite um aumento no tempo de cultivo, sem afetar a ultra-estrutura citoplasmática do oócito e sem afetar os índices de desenvolvimento embrionário, associado ao Roscovitina ou não

The aim of this study was test the effect of Cordicepim (CY), an inhibitor of mRNA translation, associate with growth factor (IGF-I), in the presence or absence of Roscovitina (Ros), a mitogen promoting factor blocker. Twelve hours in culture media with CY plus 18 or 24 hours of maturation resulted in an average of 80% of oocytes reaching metaphase II. The blastocyst yield after 12 hours culture media with CY plus 18 hours of maturation without inhibition (32.3%) was similar (P>0.05) to the control group (39.6%). A negative effect of Ros on blastocyst yield was observed when not associated to CY, (16.7% in Ros vs 49% in control group). When the concentration of Ros associated with CY was reduced, the blastocyst yield was lower (8.3%) compared with the control group (36.4%). Transmission electron microscopy evaluation showed characteristics like a mature oocyte but also some characteristics of immature oocyte after 12 hours of inhibition. In conclusion, the temporary exposure of bovine oocytes to CY associated with IGF-I during in vitro maturation allowed an increase in culture time with no effect on cytoplasm ultra-structure and blastocyst yield, associated or not with Roscovitina

Advisors/Committee Members: João Francisco Coelho de Oliveira, Marcelo Bertolini, Alceu Mezzalira.

Subjects/Keywords: cordicepim; IGF-I; roscovitina; oócito bovino; MEDICINA VETERINARIA; cordicepim; IGF-I; roscovitina; bovine oocyte

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sandri, L. R. (2007). EFEITO DE BLOQUEADORES MEIÓTICOS NA MATURAÇÃO E ULTRAESTRUTURA DE OÓCITOS E SUA CONSEQÜÊNCIA NA PRODUÇÃO DE EMBRIÕES IN VITRO. (Thesis). Universidade Federal de Santa Maria. Retrieved from http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=1087

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Sandri, Luciano Ricardo. “EFEITO DE BLOQUEADORES MEIÓTICOS NA MATURAÇÃO E ULTRAESTRUTURA DE OÓCITOS E SUA CONSEQÜÊNCIA NA PRODUÇÃO DE EMBRIÕES IN VITRO.” 2007. Thesis, Universidade Federal de Santa Maria. Accessed January 22, 2021. http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=1087.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Sandri, Luciano Ricardo. “EFEITO DE BLOQUEADORES MEIÓTICOS NA MATURAÇÃO E ULTRAESTRUTURA DE OÓCITOS E SUA CONSEQÜÊNCIA NA PRODUÇÃO DE EMBRIÕES IN VITRO.” 2007. Web. 22 Jan 2021.

Vancouver:

Sandri LR. EFEITO DE BLOQUEADORES MEIÓTICOS NA MATURAÇÃO E ULTRAESTRUTURA DE OÓCITOS E SUA CONSEQÜÊNCIA NA PRODUÇÃO DE EMBRIÕES IN VITRO. [Internet] [Thesis]. Universidade Federal de Santa Maria; 2007. [cited 2021 Jan 22]. Available from: http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=1087.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Sandri LR. EFEITO DE BLOQUEADORES MEIÓTICOS NA MATURAÇÃO E ULTRAESTRUTURA DE OÓCITOS E SUA CONSEQÜÊNCIA NA PRODUÇÃO DE EMBRIÕES IN VITRO. [Thesis]. Universidade Federal de Santa Maria; 2007. Available from: http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=1087

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Federal de Santa Maria

18. Paulo Roberto Antunes da Rosa. PROTEÍNAS LIGANTES DOS RECEPTORES DE FATORES DE CRESCIMENTO EM COMPLEXO CUMULUS-OÓCITO BOVINO.

Degree: 2011, Universidade Federal de Santa Maria

URL: http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=3983

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O objetivo do presente trabalho foi caracterizar as proteínas Grb10 e Grb14 em complexos cumulus-oócito (CCOs) de bovinos oriundos de folículos em diferentes fases de… (more)

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O objetivo do presente trabalho foi caracterizar as proteínas Grb10 e Grb14 em complexos cumulus-oócito (CCOs) de bovinos oriundos de folículos em diferentes fases de desenvolvimento e mostrar o envolvimento do estradiol na regulação da expressão de RNAm. Primeiramente, foram obtidos pool de CCOs de folículos de 3-8mm para verificar a expressão de RNAm para Grb10 e Grb14 no oócito desnudo e nas células do cumulus. Tanto o oócito quanto as células do cumulus expressaram RNAm para as proteínas em estudo. Com o intuito de caracterizar o modelo experimental utilizado, foi verificado que a competência à progressão meiótica se da em oócitos oriundos de folículos com diâmetro >2mm (P<0,01) e aumenta ao longo do desenvolvimento folicular, já que, oócitos oriundos de folículos de 1-3 e 4-6mm apresentam taxas de maturação inferiores aos oócitos oriundos de folículos de 6-8 e >8mm (P<0,05). O primeiro experimento foi delineado com o intuito de demonstrar uma expressão diferencial de mRNA para as proteínas Grb10 e Grb14 ao longo do desenvolvimento folicular. Para isso os grupos de CCOs (1-3, 4-6, 6-8 e >8mm) foram submetidos a extração RNA e transcrição reversa. A expressão relativa dos genes foi realizada por PCR em tempo real. Nesse experimento foi verificado que a expressão do gene Grb10 em CCOs esteve elevada nos grupos 1-3 e 4-6mm, diminuindo nos grupos 6-8 e >8mm (P<0,05). Já a expressão de mRNA de Grb14 esteve alta no grupo 1-3, diminuindo (P<0,05) conforme o aumento do tamanho folicular. Além disso, foi realizada a localização da proteína Grb10 pela técnica de imunofluorescência. A proteína Grb10 foi localizada tanto no oócito como no cumulus dos grupos de CCOs (1-3, 4-6, 6-8 e >8mm) porém a localização foi mais evidente em CCOs oriundos de folículos com diâmetro <6mm. Com o intuito de identificar uma influência hormonal na regulação da expressão de Grb10 e Grb14, foi realizado um terceiro experimento no qual CCOs oriundos de folículos de 3-8mm foram co-cultivados com metades foliculares em meio suplementado com estradiol-17β e/ou fulvestrant (antagonista do estradiol) durante 6h. A expressão de RNAm não apresentou diferença significativa entre os tratamentos (P>0,05). Com base nesses resultados pode-se concluir que há expressão de RNAm para Grb10 e Grb14 bem como localização da proteína Grb10 em CCOs de bovinos, e que a expressão diferencial de RNAm sugere um envolvimento desses genes na aquisição de competência oocitária ao longo do desenvolvimento folicular. Portanto novos estudos precisam ser feitos para entender um mecanismo de regulação na expressão de RNAm.

The aim of this study was to characterize the Grb10 and Grb14 mRNA and protein expression in COCs derived from follicles of different stages of development (1-3, 4-6, 6-8 and >8mm in diameter) and determinate the involvement of the estradiol in the Grb10 and/or Grb14 mRNA expression. Firstly, a pool of the 80 COCs from follicles at 3 to 8mm was used to demonstrate Grb14 and Grb10 mRNA expression in denuded oocytes and respective cumulus cells. The…

Advisors/Committee Members: Katia Padilha Barreto, Paulo Bayard Dias Goncalves, Rafael Gianella Mondadori.

Subjects/Keywords: maturação; Grb10, Grb14; MEDICINA VETERINARIA; Grb10; Grb14; maturation oocyte; bovine; bovino; oócito

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APA (6^th Edition):

Rosa, P. R. A. d. (2011). PROTEÍNAS LIGANTES DOS RECEPTORES DE FATORES DE CRESCIMENTO EM COMPLEXO CUMULUS-OÓCITO BOVINO. (Thesis). Universidade Federal de Santa Maria. Retrieved from http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=3983

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Rosa, Paulo Roberto Antunes da. “PROTEÍNAS LIGANTES DOS RECEPTORES DE FATORES DE CRESCIMENTO EM COMPLEXO CUMULUS-OÓCITO BOVINO.” 2011. Thesis, Universidade Federal de Santa Maria. Accessed January 22, 2021. http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=3983.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Rosa, Paulo Roberto Antunes da. “PROTEÍNAS LIGANTES DOS RECEPTORES DE FATORES DE CRESCIMENTO EM COMPLEXO CUMULUS-OÓCITO BOVINO.” 2011. Web. 22 Jan 2021.

Vancouver:

Rosa PRAd. PROTEÍNAS LIGANTES DOS RECEPTORES DE FATORES DE CRESCIMENTO EM COMPLEXO CUMULUS-OÓCITO BOVINO. [Internet] [Thesis]. Universidade Federal de Santa Maria; 2011. [cited 2021 Jan 22]. Available from: http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=3983.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Rosa PRAd. PROTEÍNAS LIGANTES DOS RECEPTORES DE FATORES DE CRESCIMENTO EM COMPLEXO CUMULUS-OÓCITO BOVINO. [Thesis]. Universidade Federal de Santa Maria; 2011. Available from: http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=3983

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Texas A&M University

19. Burns, Gregory Willis. Production and Functional Analysis of Recombinant Bovine Morphogenic Protein 15.

Degree: MS, Biomedical Sciences, 2013, Texas A&M University

URL: http://hdl.handle.net/1969.1/151804

► After 40 years of research, in vitro systems for mammalian embryo production produce lower quality embryos than those derived from in vivo sources. Recent reports… (more)

▼ After 40 years of research, in vitro systems for mammalian embryo production produce lower quality embryos than those derived from in vivo sources. Recent reports have demonstrated that in vitro bovine oocyte maturation systems benefit from the addition of oocyte secreted factors, specifically Bone Morphogenic Protein 15 (BMP15) from heterologous sources. However, known amino acid sequence variation and species-specific patterns of post-translational glycosylation lead us to hypothesize that utilization of bovine-specific oocyte secreted factors would be more beneficial than the observed effects of heterologous factors. To test this hypothesis, wild type, bovine BMP15 was cloned using reverse transcriptase PCR from RNA obtained from bovine ovarian tissue. For improved detection and purification of the biologically active recombinant protein, a FLAG tag peptide sequence (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) was incorporated into the wild type BMP15 gene by PCR. This modified protein was cloned into the pCDNA 3 mammalian expression vector. HEK-293 (human embryonic kidney 293) and FBK (fetal bovine kidney) cell lines were transfected via electroporation and then selected to homogeneity. Collection and purification of rbFL-BMP15 from conditioned medium was accomplished by incubation with anti-FLAG affinity gel and the use of 3X FLAG peptide for elution. Peptides of 15.4 kDa and 17 kDa were noted from the human HEK-293 transfected cell line, while in contrast, bovine FBK cells produced a single 17 kDa protein. Bioactivity and BMP receptor signaling specificity were ascertained using in vitro treatment of HeLa cells and Western blotting for the BMP signaling molecule phosphorylated-SMAD 1/5. Inhibition of this signaling cascade using dorsomorphin, a selective bone morphogenic protein receptor I inhibitor, demonstrated the purified proteins served as BMP15-like agonists. To examine the impact of our purified, bovine-specific peptides on oocyte maturation, cumulus oocyte complexes were in vitro matured for 24 hours in the presence of 100 ng/ml recombinant human BMP15 or rbFL-BMP15 from human or bovine cell lines. Real time quantitative PCR analysis of BMP15 stimulated genes, PTGS2 and TSG6, revealed statistically significant increases in transcript level for treatment with human BMP15 by a Dunnett’s test (p<0.05). In this report, however, we failed to detect a significant affect of rbFL-BMP15 on the gene expression of in vitro mature cumulus oocyte complexes at 24 hours with 100 ng/ml rbFL-BMP15. Future studies including differing time points and concentrations, along with the addition of GDF9 to form a possible heterodimer should be investigated for the possibility of improving bovine oocyte in vitro maturation. Advisors/Committee Members: Long, Charles R (advisor), Golding, Michael C (committee member), Li, Qinglei (committee member).

Subjects/Keywords: BMP15; IVM; TGFB; recombinant protein; FLAG; BMPRI; ART; cattle; bovine; COC; oocyte

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APA (6^th Edition):

Burns, G. W. (2013). Production and Functional Analysis of Recombinant Bovine Morphogenic Protein 15. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/151804

Chicago Manual of Style (16^th Edition):

Burns, Gregory Willis. “Production and Functional Analysis of Recombinant Bovine Morphogenic Protein 15.” 2013. Masters Thesis, Texas A&M University. Accessed January 22, 2021. http://hdl.handle.net/1969.1/151804.

MLA Handbook (7^th Edition):

Burns, Gregory Willis. “Production and Functional Analysis of Recombinant Bovine Morphogenic Protein 15.” 2013. Web. 22 Jan 2021.

Vancouver:

Burns GW. Production and Functional Analysis of Recombinant Bovine Morphogenic Protein 15. [Internet] [Masters thesis]. Texas A&M University; 2013. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/1969.1/151804.

Council of Science Editors:

Burns GW. Production and Functional Analysis of Recombinant Bovine Morphogenic Protein 15. [Masters Thesis]. Texas A&M University; 2013. Available from: http://hdl.handle.net/1969.1/151804

University of Saskatchewan

20. Malhi, Pritpal Singh. A bovine model to study reproductive aging.

Degree: 2007, University of Saskatchewan

URL: http://hdl.handle.net/10388/etd-06042007-170036

► Decline in fertility with age has been well documented in women. There are ethical limitations to use humans as a model for basic research, and… (more)

Subjects/Keywords: ovary; reproduction; aging; bovine model; oocyte

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APA (6^th Edition):

Malhi, P. S. (2007). A bovine model to study reproductive aging. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/etd-06042007-170036

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Malhi, Pritpal Singh. “A bovine model to study reproductive aging.” 2007. Thesis, University of Saskatchewan. Accessed January 22, 2021. http://hdl.handle.net/10388/etd-06042007-170036.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Malhi, Pritpal Singh. “A bovine model to study reproductive aging.” 2007. Web. 22 Jan 2021.

Vancouver:

Malhi PS. A bovine model to study reproductive aging. [Internet] [Thesis]. University of Saskatchewan; 2007. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/10388/etd-06042007-170036.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Malhi PS. A bovine model to study reproductive aging. [Thesis]. University of Saskatchewan; 2007. Available from: http://hdl.handle.net/10388/etd-06042007-170036

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universitat Politècnica de València

21. Cebrián Serrano, Alberto. Factors affecting the in vitro embryo production in cattle associated to ovum pick up sistem .

Degree: 2013, Universitat Politècnica de València

URL: http://hdl.handle.net/10251/27646

► La producción de embriones mediante la recuperación de ovocitos inmaduros por ovum pick up (OPU), y su posterior maduración, fecundación y cultivo en el laboratorio… (more)

▼ La producción de embriones mediante la recuperación de ovocitos inmaduros por ovum pick up (OPU), y su posterior maduración, fecundación y cultivo en el laboratorio in vitro, presenta numerosos beneficios para optimizar el potencial reproductivo, tanto de hembras como de machos. Además, frente a la superovulación convencional mediante tratamiento hormonal y la recogida de embriones in vivo, la producción in vitro de embriones (PIVE) con ovocitos de OPU ofrece considerables ventajas. Sin embargo, actualmente la PIVE continua siendo ineficiente e incapaz de producir embriones de calidad similar a los in vivo, lo cual ha limitado una aplicación más amplia de esta tecnología. Así pues, el objetivo de esta tesis fue la optimización de la PIVE en ganado vacuno, condicionado por las peculiaridades y deficiencias de la PIVE cuando los ovocitos son recuperados por la técnica de OPU. Con este fin, cinco experimento se llevaron a cabo en esta tesis. En el primero de ellos se estudió el efecto del fluido oviductal bovino (FOb) sobre el desarrollo y la calidad embrionaria (Experimento 1). Las fases del proceso de PIVE en las cuales el cultivo de ovocitos/embriones, bien individualmente o bien en número reducido, pudiera perjudicar el posterior desarrollo hasta el estadio de blastocisto y/o a su calidad, se estudiaron en el Experimento 2. En el Experimento 3 se testó si el desarrollo y la calidad de embriones cultivados in vitro en número reducido podría ser mejorada con la adición conjunta de factor de crecimiento epidérmico, insulina, transferrina y selenio (FCE-ITS) o por el sistema de cultivo de embriones llamado well of well (WOW). Las propiedades protectoras de la melatonina frente a los daños causados por el estrés oxidativo, subsecuentes de las condiciones de PIVE o de un estrés térmico durante la maduración ovocitaria, fueron evaluadas en el Experimento 4. Por último, en el Experimento 5 usamos ovocitos recolectados por OPU para evaluar el efecto del semen sexado sobre Advisors/Committee Members: Salvador Vidal, Ignacio (advisor), Silvestre Camps, Miguel Ángel (advisor).

Subjects/Keywords: Embryo; Oocyte; Bovine; Ovum pick up; In vitro fetilization; In vitro production of embryos

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Cebrián Serrano, A. (2013). Factors affecting the in vitro embryo production in cattle associated to ovum pick up sistem . (Doctoral Dissertation). Universitat Politècnica de València. Retrieved from http://hdl.handle.net/10251/27646

Chicago Manual of Style (16^th Edition):

Cebrián Serrano, Alberto. “Factors affecting the in vitro embryo production in cattle associated to ovum pick up sistem .” 2013. Doctoral Dissertation, Universitat Politècnica de València. Accessed January 22, 2021. http://hdl.handle.net/10251/27646.

MLA Handbook (7^th Edition):

Cebrián Serrano, Alberto. “Factors affecting the in vitro embryo production in cattle associated to ovum pick up sistem .” 2013. Web. 22 Jan 2021.

Vancouver:

Cebrián Serrano A. Factors affecting the in vitro embryo production in cattle associated to ovum pick up sistem . [Internet] [Doctoral dissertation]. Universitat Politècnica de València; 2013. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/10251/27646.

Council of Science Editors:

Cebrián Serrano A. Factors affecting the in vitro embryo production in cattle associated to ovum pick up sistem . [Doctoral Dissertation]. Universitat Politècnica de València; 2013. Available from: http://hdl.handle.net/10251/27646

Utah State University

22. Bayles, Ammon Hanson. Mechanisms and Signal Transduction Pathways Involved in Bovine Oocyte Activation.

Degree: PhD, Animal, Dairy, and Veterinary Sciences, 2012, Utah State University

URL: https://digitalcommons.usu.edu/etd/1380

► In addition to contributing genes at fertilization, the sperm cell induces the oocyte to leave its arrested state and resume metabolism in the process… (more)

Subjects/Keywords: bovine; fertilization; Oocyte Activation; Phospholipase C; Signal Transduction; Src Family Kinase; Animal Sciences

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APA (6^th Edition):

Bayles, A. H. (2012). Mechanisms and Signal Transduction Pathways Involved in Bovine Oocyte Activation. (Doctoral Dissertation). Utah State University. Retrieved from https://digitalcommons.usu.edu/etd/1380

Chicago Manual of Style (16^th Edition):

Bayles, Ammon Hanson. “Mechanisms and Signal Transduction Pathways Involved in Bovine Oocyte Activation.” 2012. Doctoral Dissertation, Utah State University. Accessed January 22, 2021. https://digitalcommons.usu.edu/etd/1380.

MLA Handbook (7^th Edition):

Bayles, Ammon Hanson. “Mechanisms and Signal Transduction Pathways Involved in Bovine Oocyte Activation.” 2012. Web. 22 Jan 2021.

Vancouver:

Bayles AH. Mechanisms and Signal Transduction Pathways Involved in Bovine Oocyte Activation. [Internet] [Doctoral dissertation]. Utah State University; 2012. [cited 2021 Jan 22]. Available from: https://digitalcommons.usu.edu/etd/1380.

Council of Science Editors:

Bayles AH. Mechanisms and Signal Transduction Pathways Involved in Bovine Oocyte Activation. [Doctoral Dissertation]. Utah State University; 2012. Available from: https://digitalcommons.usu.edu/etd/1380

Louisiana State University

23. Len Yin, Jose. The Ability of Bull and Stallion Thawed Spermatozoa Refrozen without Cryoprotectants to Activate Intra- and Interspecies Oocytes.

Degree: PhD, Veterinary Medicine, 2016, Louisiana State University

URL: etd-07082016-081805 ; https://digitalcommons.lsu.edu/gradschool_dissertations/2508

► Semen cryopreservation has allowed the establishment of genome banks and the large scale propagation of species. The development of simple techniques to cryopreserve semen or… (more)

▼ Semen cryopreservation has allowed the establishment of genome banks and the large scale propagation of species. The development of simple techniques to cryopreserve semen or alternatives to efficiently use cryopreserved semen from males of valuable genetics that have become infertile will permit continuous propagation of the genetics from these males and may serve as a model for preservation and propagation of endangered species. Sperm cryopreservation without cryoprotectants is a simple process, and offspring have been produced following intracytoplasmic sperm injection (ICSI); however the ability of frozen-thawed sperm refrozen without the addition of cryoprotectants to activate oocytes following ICSI was unknown. In the series of experiments performed, bull and stallion frozen-thawed sperm refrozen without the addition of cryoprotectants was used to activate intra- and interspecies oocyte following ICSI. Additionally, equine cumulus-oocyte complexes (COCs) glucose metabolism during in vitro maturation was evaluated. The first experiment demonstrated that bull and stallion frozen-thawed sperm refrozen without the addition of cryoprotectants had their plasma membrane damaged; however the DNA was unaffected. The second experiment demonstrated that bull and stallion frozen-thawed sperm refrozen without the addition of cryoprotectants had the ability to activate bovine oocytes following intracytoplasmic sperm injection; although at a lower rate compared to frozen-thawed sperm. The third experiment demonstrated that frozen-thawed stallion sperm refrozen without the addition of cryoprotectants was unable to activate equine oocytes. The exact reason for this failure could not be explained from the experiment; however COC metabolism during in vitro maturation impacts embryo activation/development and required further investigation. The fourth experiment demonstrated that equine COCs consume and metabolize glucose through glycolysis during in vitro maturation; however, results from this experiment were unable to explain the failure of refrozen stallion sperm to activate equine oocytes. To our knowledge, this is the first report of the use of bull or stallion frozen-thawed sperm refrozen without the addition of cryoprotectants to activate oocytes following ICSI. Furthermore, this is also the first report of equine COCs glucose metabolism during in vitro maturation.

Subjects/Keywords: cryoprotectants; sperm; ICSI; refrozen; oocyte activation; bull; stallion; equine; bovine; plasma membrane; DNA

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APA (6^th Edition):

Len Yin, J. (2016). The Ability of Bull and Stallion Thawed Spermatozoa Refrozen without Cryoprotectants to Activate Intra- and Interspecies Oocytes. (Doctoral Dissertation). Louisiana State University. Retrieved from etd-07082016-081805 ; https://digitalcommons.lsu.edu/gradschool_dissertations/2508

Chicago Manual of Style (16^th Edition):

Len Yin, Jose. “The Ability of Bull and Stallion Thawed Spermatozoa Refrozen without Cryoprotectants to Activate Intra- and Interspecies Oocytes.” 2016. Doctoral Dissertation, Louisiana State University. Accessed January 22, 2021. etd-07082016-081805 ; https://digitalcommons.lsu.edu/gradschool_dissertations/2508.

MLA Handbook (7^th Edition):

Len Yin, Jose. “The Ability of Bull and Stallion Thawed Spermatozoa Refrozen without Cryoprotectants to Activate Intra- and Interspecies Oocytes.” 2016. Web. 22 Jan 2021.

Vancouver:

Len Yin J. The Ability of Bull and Stallion Thawed Spermatozoa Refrozen without Cryoprotectants to Activate Intra- and Interspecies Oocytes. [Internet] [Doctoral dissertation]. Louisiana State University; 2016. [cited 2021 Jan 22]. Available from: etd-07082016-081805 ; https://digitalcommons.lsu.edu/gradschool_dissertations/2508.

Council of Science Editors:

Len Yin J. The Ability of Bull and Stallion Thawed Spermatozoa Refrozen without Cryoprotectants to Activate Intra- and Interspecies Oocytes. [Doctoral Dissertation]. Louisiana State University; 2016. Available from: etd-07082016-081805 ; https://digitalcommons.lsu.edu/gradschool_dissertations/2508

University of Guelph

24. Carvajal, Diana. Role of Hippo Signaling Pathway During Bovine Oocyte Maturation In-Vitro.

Degree: MS, Department of Biomedical Sciences, 2018, University of Guelph

URL: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/14579

► Oocyte development is strictly regulated by several ovarian signaling pathways. One of these pathways is the Hippo signaling pathway, known for its role in cell… (more)

Subjects/Keywords: Hippo signaling pathway; MST1/2; LATS1/2; YAP; TAZ; Oocyte maturation; Bovine

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APA (6^th Edition):

Carvajal, D. (2018). Role of Hippo Signaling Pathway During Bovine Oocyte Maturation In-Vitro. (Masters Thesis). University of Guelph. Retrieved from https://atrium.lib.uoguelph.ca/xmlui/handle/10214/14579

Chicago Manual of Style (16^th Edition):

Carvajal, Diana. “Role of Hippo Signaling Pathway During Bovine Oocyte Maturation In-Vitro.” 2018. Masters Thesis, University of Guelph. Accessed January 22, 2021. https://atrium.lib.uoguelph.ca/xmlui/handle/10214/14579.

MLA Handbook (7^th Edition):

Carvajal, Diana. “Role of Hippo Signaling Pathway During Bovine Oocyte Maturation In-Vitro.” 2018. Web. 22 Jan 2021.

Vancouver:

Carvajal D. Role of Hippo Signaling Pathway During Bovine Oocyte Maturation In-Vitro. [Internet] [Masters thesis]. University of Guelph; 2018. [cited 2021 Jan 22]. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/14579.

Council of Science Editors:

Carvajal D. Role of Hippo Signaling Pathway During Bovine Oocyte Maturation In-Vitro. [Masters Thesis]. University of Guelph; 2018. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/14579

Massey University

25. Li, Dongxing. Oxygen consumption of bovine granulosa cells in vitro.

Degree: M. Eng., Biotechnology, 2012, Massey University

URL: http://hdl.handle.net/10179/3986

► The oxygen consumption rate of granulosa cells is considered to be a key determinant of oocyte oxygenation in follicles. The oxygen status of the oocyte… (more)

▼ The oxygen consumption rate of granulosa cells is considered to be a key determinant of oocyte oxygenation in follicles. The oxygen status of the oocyte potentially dictates its developmental competence. However, quantitative information on the oxygen consumption rate of granulosa cells in literature is scarce. This limitation has hindered further investigation into the oocyte oxygenation, which could potentially be used as an indicator for selecting high quality oocytes for producing high quality embryos. This could ultimately contribute to improvement of the success rates of human In-Vitro Fertilisation. In light of this issue, this work developed a method for measuring the oxygen consumption rate of granulosa cells in vitro. This included techniques related to granulosa cell harvest from cows, suspending/culturing granulosa cells in culture medium and development of a competent respirometer. Each measurement run on the oxygen consumption rate of granulosa cells was conducted by suspending the granulosa cell culture in the respirometer, in which an optical-based oxygen sensor probe was employed to continuously monitor the oxygen partial pressure change in the cell suspension. Five separate sets of respirometer data were collected and used to calculate the oxygen consumption rate, giving a range of 2.1 to 3.3 x 10-16mol.cell-1.s-1/0.16 to 0.25mol.m-3.s-1. These rates were comparable with but higher than other animal cell oxygen consumption rates reported by the literature. They were approximately 5 times higher than the oxygen consumption rate of granulosa cells harvested from sheep (Gosden & Byatt-Smith 1986). The implications of the measured oxygen consumption rate were then examined in the context of oxygen transport in large bovine preantral follicles via an existing mathematical model. The resulting predicted oxygen profiles in large bovine follicles were consistent with the study of Redding et al. (2007), which showed that as a preantral follicle grew the oxygen transport across the follicle was increasingly strained, resulting in subsequent decrease in oocyte oxygenation. By applying the bovine specific parameter estimates to the model, this work predicted that the largest follicle radius for the oxygen transport in bovine preantral follicle was 134μm, beyond which the oxygen could not reach oocyte. Since the experimentally reported sizes of the large bovine preantral follicles ranged of 58 to 145μm in radius (see Section 7.1.2), this work proposed that the oxygen transport was capable of oxygenating the oocytes in all but the largest preantral follicles. If bovine preantral follicles were to grow larger than they are experimentally observed to do so, all oocytes contained within such follicles would be in a hypoxic state. This is a result consistent with other work. Furthermore, based on the use of bovine and ovine specific parameter estimates in the model, this work found that oxygen transport in follicles was likely to be the result of a unique combination of parameters for a particular species.…

Subjects/Keywords: Oxygen consumption; Granulosa cells; Oocytes; Oxygen consumption measurement; Bovine follicles; Oocyte oxygenation; Preantral follicles

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Li, D. (2012). Oxygen consumption of bovine granulosa cells in vitro. (Masters Thesis). Massey University. Retrieved from http://hdl.handle.net/10179/3986

Chicago Manual of Style (16^th Edition):

Li, Dongxing. “Oxygen consumption of bovine granulosa cells in vitro.” 2012. Masters Thesis, Massey University. Accessed January 22, 2021. http://hdl.handle.net/10179/3986.

MLA Handbook (7^th Edition):

Li, Dongxing. “Oxygen consumption of bovine granulosa cells in vitro.” 2012. Web. 22 Jan 2021.

Vancouver:

Li D. Oxygen consumption of bovine granulosa cells in vitro. [Internet] [Masters thesis]. Massey University; 2012. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/10179/3986.

Council of Science Editors:

Li D. Oxygen consumption of bovine granulosa cells in vitro. [Masters Thesis]. Massey University; 2012. Available from: http://hdl.handle.net/10179/3986

26. Schwarz, Kátia Regina Lancellotti. O óxido nítrico e os nucleotídeos cíclicos em oócitos bovinos maturados in vitro.

Degree: PhD, Qualidade e Produtividade Animal, 2011, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/74/74131/tde-03072012-091027/ ;

►

O óxido nítrico (NO) é um mensageiro químico gerado pela atividade da enzima óxido nítrico sintase (NOS) a qual foi detectada em vários órgãos incluído… (more)

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O óxido nítrico (NO) é um mensageiro químico gerado pela atividade da enzima óxido nítrico sintase (NOS) a qual foi detectada em vários órgãos incluído o sistema reprodutor. O sistema NOS/NO parece desempenhar papel importante na maturação oocitária entre outras funções. No entanto, apesar das evidências, há poucos estudos sobre o papel desse sistema em oócitos da espécie bovina. Sabe-se que o NO atua pela via da guanilato ciclase (GC) estimulando a produção do nucleotídeo GMPc, que por sua vez é capaz de influenciar os níveis de outro nucleotídeo, o AMPc, que é um importante elemento da via de sinalização das gonadotrofinas nos oócitos e no controle da maturação oocitária. O objetivo do presente estudo foi de investigar o envolvimento da via do GMPc na ação do sistema NOS/NO na maturação in vitro (MIV) de oócitos bovinos e seu efeito sobre a via do AMPc. Com a maior concentração estudada do doador de NO (10-7M de SNAP), apenas 36% dos oócitos conseguiram alcançar o estágio de RVG (P< 0,05), após 9 horas de maturação. Esse atraso também foi observado com diferentes concentrações do estimulador de GC (5, 10 ou 50μM de Proptoporfirina IX) e pelo análogo de GMPc (1, 2 e 4mM de 8-Br-GMPc ), que apresentaram uma taxa média de RVG de 50% para os tratamentos e 70% para os grupos controles sem as drogas (P<0,05). No início da maturação (0h), os níveis de GMPc foram de 5,29 pmol/oócito sofrendo uma queda logo na primeira hora de cultivo para 2,97 pmol nos oócitos do grupo controle e 1,54 pmol nos cultivados com associação de 10-7M de SNAP (doador de NO) e 100μM de OQD (inibidor de GC (P<0,05). No grupo de oócitos cultivados apenas com SNAP, os níveis de GMPc se mantiveram em 4,51 pmol/oócito, semelhante ao grupo imaturo (0h de cultivo, P>0,05). O doador de NO manteve estável o nível de GMPc somente na primeira hora de maturação. Após 3 e 6 h de MIV, os níveis de GMPc permaneceram baixos e similares (0,07 a 2,46 pmol/oócito, P>0,05) nos grupos controle (sem drogas), tratado com doador de NO (10-7M de SNAP) associado ou não ao inibidor de guanilato ciclase (100μM de OQD). Também foi observada uma queda nos níveis de AMPc em relação ao grupo imaturo (32,42 fmol de AMPc/oócito) para os demais grupos (P<0,05), que apresentaram aproximadamente, 12,0 a 16,0 fmol de AMPc/oócito durante a primeira hora, 3,3 a 8,0 fmol/oócito durante a terceira hora e 7,4 a 18,3 durante a sexta hora de maturação (P>0,05). O NO afetou os níveis de GMPc no início da maturação, mas não os níveis de AMPc. O NO e o GMPc podem atuar no controle da expressão gênica de uma série de proteínas envolvidas no controles dos níveis de AMPc e GMPc ou suas funções. Esse controle pode ser efeito direto do NO (PKG2, PDE3A, PDE4D e PDE8A), do GMPc (ADCY6) ou do NO via GMPc (PKA1) e varia com o compartimento considerado (oócito ou células do cumulus). Esses resultados demonstraram a inter-relação das vias NO/GMPc/AMPc e toda a sua complexidade dependendo do tipo celular e da fase da maturação de oócitos bovinos.

The NOS/NO system seems to play an important…

Advisors/Committee Members: Leal, Cláudia Lima Verde.

Subjects/Keywords: In vitro maturation; Bovine oocyte; Maturação in vitro; Meiose; Meiosis; Nitric oxide; Oócito bovino; Óxido nítrico

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Schwarz, K. R. L. (2011). O óxido nítrico e os nucleotídeos cíclicos em oócitos bovinos maturados in vitro. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/74/74131/tde-03072012-091027/ ;

Chicago Manual of Style (16^th Edition):

Schwarz, Kátia Regina Lancellotti. “O óxido nítrico e os nucleotídeos cíclicos em oócitos bovinos maturados in vitro.” 2011. Doctoral Dissertation, University of São Paulo. Accessed January 22, 2021. http://www.teses.usp.br/teses/disponiveis/74/74131/tde-03072012-091027/ ;.

MLA Handbook (7^th Edition):

Schwarz, Kátia Regina Lancellotti. “O óxido nítrico e os nucleotídeos cíclicos em oócitos bovinos maturados in vitro.” 2011. Web. 22 Jan 2021.

Vancouver:

Schwarz KRL. O óxido nítrico e os nucleotídeos cíclicos em oócitos bovinos maturados in vitro. [Internet] [Doctoral dissertation]. University of São Paulo; 2011. [cited 2021 Jan 22]. Available from: http://www.teses.usp.br/teses/disponiveis/74/74131/tde-03072012-091027/ ;.

Council of Science Editors:

Schwarz KRL. O óxido nítrico e os nucleotídeos cíclicos em oócitos bovinos maturados in vitro. [Doctoral Dissertation]. University of São Paulo; 2011. Available from: http://www.teses.usp.br/teses/disponiveis/74/74131/tde-03072012-091027/ ;

Universidade Federal de Viçosa

27. Letícia Martins Fagundes. Congelação de ovócitos desnudados ou não, maturos e imaturos de bovinos, utilizando o etileno glicol pelo método convencional.

Degree: 2002, Universidade Federal de Viçosa

URL: http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=5658

► This study aimed at the evaluation of the effects from cryopreservation on the in vitro matured and immature oocytes, using the conventional method. The experiment… (more)

▼ This study aimed at the evaluation of the effects from cryopreservation on the in vitro matured and immature oocytes, using the conventional method. The experiment was carried out in the Animal Reproduction Laboratory - DVT-UFV, by using oocytes from cows ovaries from slaughterhouse, which were distributed into six treatments. Treatment 1 (T1): non-frozen oocytes (fresh ones) provided with the cumulus oophorus cells (COC) which were immediately submitted to the processes MIV, FIV and CIV. Treatment 2 (T2): non-frozen oocytes (fresh ones), deprived unprovided of the cumulus oophorus cells (naked), which were immediately submitted to MIV, FIV and CIV. Treatment 3 (T3): immature oocytes provided with the cumulus oophorus cells, which were submitted to cryopreservation just after selection. Later, they were thawed and those considered as normal ones were submitted to MIV, FIV and CIV. Treatment 4 (T4): naked oocytes submitted to cryopreservation. Later, they were thawed and those considered as normal ones were submitted to MIV, FIV and CIV. Treatment 5 (T5): oocytes provided with the cumulus oophorus cells, matured in vitro, and then they were frozen. Later, they were thawed and those considered as normal ones were submitted to recultivation for 2 hours in a maturation medium, and then submitted to FIV and CIV. Treatment 6 (T6): naked, in vitro matured oocytes which were submitted to cryopreservation. After thawing, those oocytes which were considered as normal ones were submitted to recultivation for 2 hours, and then to FIV and CIV. Those oocytes of the treatments 3, 4, 5 and 6 were frozen in a solution containing 1.8 mol L-1 ethylene glycol (EG) in Talp-Hepes (base medium) added with 0.4% BSA-V by the conventional method. The oocytes were dehydrated by immersion into three solutions (phases) at increasing concentrations of the cryoproctetor, (0.6; 1.2 and 1.8 mol L-1 of EG) during a maximum permanence time of five minutes for each phase, at environmental temperature. Thawing was accomplished by immersion into water bath at 30 °C for 20 seconds. Later, the oocytes were rehydrated at three stages, that is, 0.9 mol L-1 EG + 0.3 mol L -1 sucrose, 0.3 mol L-1 sucrose, and without both EG and sucrose for six minutes each one. After thawing, the oocytes recovery rates were 92.6, 97.5, 96.6 and 93,2% for the treatments 3, 4, 5 and 6, respectively. In relation to the recovered oocytes, the following were also found: a cytoplasm withdrawal rate of 3.3, 0.8, 0.3 and 6.2%, and a cellular content loss of 2.2, 13.1, 3.8 and 16.1%, also for treatments 3, 4, 5 and 6, respectively. The frozen immature oocytes presented a maturation rate of 9.2 and 5.8% (treatments 3 and 4, respectively); these results were much inferior to those obtained by the fresh oocytes, that is, 82.5 (T1) and 75.4% (T2). The fecundation rates were 56.2, 0.0, 38.7, 8.6, 63.6 and 16.7%, while the clivage rates were 36.3, 7.9, 0.4, 0.0, 0.0, and 0.0% for treatments 1, 2, 3, 4, 5 and 6, respectively. Only the unfrozen and no-naked oocytes (T1) exhibited development… Advisors/Committee Members: Ciro Alexandre Alves Torres, Eduardo Paulino da Costa, José Domingos Guimarães, Giovanni Ribeiro de Carvalho, Tarcízio Antônio Rego de Paula.

Subjects/Keywords: REPRODUCAO ANIMAL; Criopreservação; Ovócito; Bovino; Cryopreservation; Oocyte; Bovine

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Fagundes, L. M. (2002). Congelação de ovócitos desnudados ou não, maturos e imaturos de bovinos, utilizando o etileno glicol pelo método convencional. (Thesis). Universidade Federal de Viçosa. Retrieved from http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=5658

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Fagundes, Letícia Martins. “Congelação de ovócitos desnudados ou não, maturos e imaturos de bovinos, utilizando o etileno glicol pelo método convencional.” 2002. Thesis, Universidade Federal de Viçosa. Accessed January 22, 2021. http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=5658.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Fagundes, Letícia Martins. “Congelação de ovócitos desnudados ou não, maturos e imaturos de bovinos, utilizando o etileno glicol pelo método convencional.” 2002. Web. 22 Jan 2021.

Vancouver:

Fagundes LM. Congelação de ovócitos desnudados ou não, maturos e imaturos de bovinos, utilizando o etileno glicol pelo método convencional. [Internet] [Thesis]. Universidade Federal de Viçosa; 2002. [cited 2021 Jan 22]. Available from: http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=5658.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Fagundes LM. Congelação de ovócitos desnudados ou não, maturos e imaturos de bovinos, utilizando o etileno glicol pelo método convencional. [Thesis]. Universidade Federal de Viçosa; 2002. Available from: http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=5658

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade de Brasília

28. Marcelo Tigre Moura. Utilização da actinomicina D como método de enucleação química de ovócitos bovinos destinados à transferência nuclear.

Degree: 2007, Universidade de Brasília

URL: http://bdtd.bce.unb.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=2088

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A clonagem por Transferência Nuclear (TN) é uma técnica pouco eficiente e laboriosa. O processo, do ponto de vista técnico, é um dos principais fatores… (more)

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A clonagem por Transferência Nuclear (TN) é uma técnica pouco eficiente e laboriosa. O processo, do ponto de vista técnico, é um dos principais fatores responsáveis pela quantidade e qualidade dos embriões produzidos. A enucleação é a etapa mais agressiva da TN. Apesar dos métodos alternativos propostos, a enucleação permanece sendo realizada como inicialmente descrita nos anos 50. O objetivo do presente trabalho foi avaliar o efeito do inibidor irreversível de transcrição e replicação actinomicina D (Fluka, Suiça) como método de enucleação química de ovócitos. Ovócitos foram maturados in vitro (MIV) por 4 ou 6 horas e expostos a actinomicina D (T1, controle; T2 = 1,0 μg ml- / 16hs; T3 = 1,0 μg ml- / 14hs; T4 = 2,5 μg ml- / 14hs; T5 = 5,0 μg ml- / 14hs). Os ovócitos foram desnudados após 20 horas de MIV e ativados com 24-26 horas de MIV. As taxas de clivagem foram avaliadas 48 horas após a ativação (D2) e as de blastocisto após 168 (D7) e 192 (D8) horas de cultivo. Parte dos ovócitos foi fixada e corada com lacmóide ou orceína para determinação da fase da meiose ou para visualização da morfologia dos cromossomos, respectivamente. Posteriormente, ovócitos tratados com actinomicina D foram utilizados para clonagem por TN, sendo que parte dos embriões (D3) foi fixada para avaliar o percentual de células apoptóticas através do teste de TUNEL. Os dados relativos às taxas de maturação, clivagem e blastocisto foram analisados pelo teste do Qui-quadrado e os percentuais de apoptose pelo teste de Mann-Whitney. A taxa de maturação (T1 = 90,4%; T2 = 82,3%; T3 = 79,1%; T4 = 83,4%; T5 = 74,7%), clivagem (T1 = 68,9%; T2 = 46,0%; T3 = 49,7%; T4= 33,4; T5= 29,3%) e de blastocisto em D8 (T1 = 41,1%; T2 = 1,4%; T3= 1,3%; T4= 0,9%; T5= 0,0%) após o tratamento com actinomicina D foi significativamente menor. Dos ovócitos fixados, 63,2% estavam em metáfase II (MII). Ao avaliar os cromossomos, foi observada uma descondensação significativa com as concentrações mais altas (2,5 e 5,0 μg ml-). Os demais tratamentos não apresentaram modificações perceptíveis. Ovócitos tratados com 1,0 μg ml- por 14 horas foram utilizados como citoplasmas receptores. A taxa de clivagem dos embriões reconstruídos (61,3%) foi semelhante ao grupo partenogenético tratado (61,3%) e inferior ao não tratado com actinomicina D (70,2%), embora a taxa de blastocisto tenha sido mais alta no grupo de TN (11,8%) em relação ao controle tratado (3,6%) e inferior ao controle sem tratamento (38,0%). Ao analisar o índice apoptótico dos embriões, os embriões partenogenéticos tratados tiveram um índice maior que os partenogenéticos não tratados (24,2% contra 4,8%). No entanto, os embriões oriundos de TN com ovócito tratado não foram diferentes estatisticamente dos TN controle (9,3 contra 13,0%). A actinomicina D é eficiente no bloqueio da transcrição e replicação embrionária. Além disso, foi possível obter embriões reconstruídos que apresentavam percentual de células apoptóticas indistinguível do controle.

Cloning by Nuclear Transfer (NT) is a low…

Advisors/Committee Members: Rodolfo Rumpf, Maurício Machaim Franco, Carolina Madeira Lucci.

Subjects/Keywords: ovócito; oocyte; transferência nuclear; bovino; enucleação; actinomicina D; REPRODUCAO ANIMAL; bovine; enucleation; actinomycin D; nuclear transfer.

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Moura, M. T. (2007). Utilização da actinomicina D como método de enucleação química de ovócitos bovinos destinados à transferência nuclear. (Thesis). Universidade de Brasília. Retrieved from http://bdtd.bce.unb.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=2088

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Moura, Marcelo Tigre. “Utilização da actinomicina D como método de enucleação química de ovócitos bovinos destinados à transferência nuclear.” 2007. Thesis, Universidade de Brasília. Accessed January 22, 2021. http://bdtd.bce.unb.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=2088.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Moura, Marcelo Tigre. “Utilização da actinomicina D como método de enucleação química de ovócitos bovinos destinados à transferência nuclear.” 2007. Web. 22 Jan 2021.

Vancouver:

Moura MT. Utilização da actinomicina D como método de enucleação química de ovócitos bovinos destinados à transferência nuclear. [Internet] [Thesis]. Universidade de Brasília; 2007. [cited 2021 Jan 22]. Available from: http://bdtd.bce.unb.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=2088.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Moura MT. Utilização da actinomicina D como método de enucleação química de ovócitos bovinos destinados à transferência nuclear. [Thesis]. Universidade de Brasília; 2007. Available from: http://bdtd.bce.unb.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=2088

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Federal de Santa Maria

29. Luciana Benetti. RECEPTOR DE ANGIOTENSINA II ENVOLVIDO NA REGULAÇÃO DA MATURAÇÃO NUCLEAR DE OÓCITO BOVINO.

Degree: 2008, Universidade Federal de Santa Maria

URL: http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=2022

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O objetivo deste estudo foi de verificar o tipo de receptor da angiotensina II (AngII) envolvido na maturação nuclear de oócitos bovinos e a possível… (more)

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O objetivo deste estudo foi de verificar o tipo de receptor da angiotensina II (AngII) envolvido na maturação nuclear de oócitos bovinos e a possível participação da bradicinina (BK) como mediadora da AngII nesse processo. O primeiro experimento foi conduzido para analisar o tipo de receptor, para isso os complexos cumulus-oócitos (CCOs) foram cultivados por 7 ou 12h na presença de hemiseções foliculares e AngII (10-11M), com ou sem os antagonistas losartan (10-6M; seletivo para receptores AT1), PD123319 (10-6M; seletivo para receptores AT2) ou saralasina (10-7M; antagonista AT1 e AT2). Adicionalmente, foram utilizados dois grupos controle, onde os oócitos foram cultivados na ausência ou na presença de hemiseções foliculares (controle positivo e negativo, respectivamente). Oócitos cultivados por 7h em presença de AngII atingiram 70,4% de rompimento da vesícula germinativa (RVG). Quando o losartan foi adicionado ao meio de cultivo com AngII, não houve diferença na percentagem de oócitos em RVG (73,2%), porém, quando os CCOs foram incubados na presença de AngII + PD123319, houve queda significativa na maturação nuclear (52,1%; P<0,05). O cultivo dos oócitos por um período maior (12h) não causou diferença significativa em relação a esses tratamentos quando foram realizados às 7h (P<0,05). Esses dados indicam que os receptores AT2 mediam as ações da AngII na maturação nuclear em bovinos. Em um segundo experimento, foi verificado o efeito de diferentes concentrações de PD123319 na maturação nuclear dos oócitos na presença de AngII (10-11M) e hemiseções foliculares. O cultivo foi realizado por 7h em um delineamento similar ao do experimento anterior e verificou-se que a inibição induzida pelo antagonista ocorreu de maneira dosedependente e há relação linear entre concentração de PD e índices de RVG obtidos (P=0,0306). Para verificar a participação da BK no mecanismo de ação dos receptores AT2, os oócitos foram cultivados na presença de AngII (10-11M) e hemiseções foliculares com ou sem Hoe-140 (antagonista de receptores B2 da BK) em diferentes concentrações (10-6, 10-7, 10-8 e 10-9M) por 7h. Nesse experimento, não houve diferença nos índices de oócitos que atingiram RVG entre os grupos tratados com o antagonista e o grupo AngII (P>0,05). Esses dados permitem inferir que a AngII atua na maturação nuclear dos oócitos bovinos ativando os receptores AT2 e que a via BK/receptor B2 não está envolvida nesse processo.

The aim of this study was to verify the type of angiotensin II (AngII) receptor involved in nuclear maturation of bovine oocytes and the possible participation of bradykinin (BK) as a mediator of AngII in this process. The first experiment was conducted to analyze the type of AngII receptor, for this cumulus-oocyte complexes (COCs) were cultured for 7 or 12h in the presence of follicular hemisections and AngII (10-11M), with or without the antagonists losartan (10-6M; selective for AT1 receptor), PD123319 (10-6M; selective for AT2 receptor) or saralasin (10-7M; AT1 and AT2 antagonist). Additionally, two control…

Advisors/Committee Members: Katia Padilha Barreto, Juliano Ferreira, José Ricardo de Figueiredo.

Subjects/Keywords: oócito bovino; angiotensina II; receptor AT2; bradicinina; maturação nuclear; FARMACIA; oocyte bovine; angiotensin II; AT2 receptor; bradykinin; nuclear maturation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Benetti, L. (2008). RECEPTOR DE ANGIOTENSINA II ENVOLVIDO NA REGULAÇÃO DA MATURAÇÃO NUCLEAR DE OÓCITO BOVINO. (Thesis). Universidade Federal de Santa Maria. Retrieved from http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=2022

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Benetti, Luciana. “RECEPTOR DE ANGIOTENSINA II ENVOLVIDO NA REGULAÇÃO DA MATURAÇÃO NUCLEAR DE OÓCITO BOVINO.” 2008. Thesis, Universidade Federal de Santa Maria. Accessed January 22, 2021. http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=2022.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Benetti, Luciana. “RECEPTOR DE ANGIOTENSINA II ENVOLVIDO NA REGULAÇÃO DA MATURAÇÃO NUCLEAR DE OÓCITO BOVINO.” 2008. Web. 22 Jan 2021.

Vancouver:

Benetti L. RECEPTOR DE ANGIOTENSINA II ENVOLVIDO NA REGULAÇÃO DA MATURAÇÃO NUCLEAR DE OÓCITO BOVINO. [Internet] [Thesis]. Universidade Federal de Santa Maria; 2008. [cited 2021 Jan 22]. Available from: http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=2022.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Benetti L. RECEPTOR DE ANGIOTENSINA II ENVOLVIDO NA REGULAÇÃO DA MATURAÇÃO NUCLEAR DE OÓCITO BOVINO. [Thesis]. Universidade Federal de Santa Maria; 2008. Available from: http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=2022

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Federal de Santa Maria

30. Jerônimo Rubert Stefanello. ANGIOTENSINA II E SUA ASSOCIAÇÃO COM FATOR-I DE CRESCIMENTO SEMELHANTE À INSULINA, INSULINA E CÉLULAS FOLICULARES NA MATURAÇÃO DE OÓCITOS BOVINOS E CONSEQÜENTE DESENVOLVIMENTO EMBRIONÁRIO.

Degree: 2005, Universidade Federal de Santa Maria

URL: http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=317

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The aim of the present study was to evaluate the action of angiotensin II (Ang II), insulin-like growth factor-I (IGF-I) and insulin (Ins) on bovine… (more)

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The aim of the present study was to evaluate the action of angiotensin II (Ang II), insulin-like growth factor-I (IGF-I) and insulin (Ins) on bovine oocyte nuclear and cytoplasmic maturation with follicular cells. Cumulus-oocyte complexes (COCs) were cultured for 22 h with follicular cells and Ang II, IGF-I or Ins. In this experiment, two control groups were used, where the oocytes were cultured with (control with cells) or without (control without cells) follicular cells. Two experiments were performed with these five groups, in the presence or absence of LH and FSH. Only the oocytes cultured in the Ang II group (88.21.8 and 90.74.3%), respectively without and with LH/FSH) had resumption of meiosis in similar rates than those cultured in the absence of follicular cells (89.70.3 and 92.62.6; P<0.01). In a second experiment, the action of Ang II and its association with IGF-I or insulin on oocyte meiotic maturation was observed for 7 (germinal vesicle breakdown - GVBD), 12 (metaphase I - MI) and 22 (metaphase II - MII) hours, in a similar experimental design of the previous experiment. The Ang II, Ang II + IGF-I and Ang II + Ins, independent of gonadotrophins, were able to overturns the inhibition of meiotic maturation caused by follicular cells, in the same rates of control group without cells, in the three evaluated period (P<0.01). One last study was performed to investigate the effect of follicular cells, Ang II, IGF-I, Ins and its associations during oocyte maturation on the rate of subsequent embryo development. The oocytes were submitted to maturation similarly to the second experiment for 1 h (1+23 h), 12 h (12+12 h) or 24 h in the presence of follicular cells and its respective treatments + the period to complete 24 h in maturation media. In 1 + 23 h, the cleavage, blastocyst and hatching rates were not different among these five groups. In 12 + 12 h, the oocytes matured in the Ang II + IGF-I group developed into blastocyst (43.80.6) and hatching/total oocytes (17.11.2) in a higher rates than the others treatments (P<0.05). In 24 h, the association of Ang II + IGF-I improved blastocyst rate in comparison to the others treatments with follicular cells (P<0.05). Also, only in this group hatched blastocysts were obtained after 24 h in the presence of follicular cells. In conclusion, Ang II overturned the inhibitory effect on bovine oocyte nuclear maturation caused by follicular cells, independent of the presence of gonadotrophins, IGF-I and insulin. However, the oocyte cytoplasmic maturation evaluated through embryo development was improved when the Ang II and IGF-I were presents in maturation media with follicular cells for 12 + 12 hours.

O objetivo do presente estudo foi de avaliar a ação da angiotensina II (Ang II), fator-I de crescimento semelhante à insulina (IGF-I) e insulina (Ins) na maturação nuclear e citoplasmática de oócitos bovinos na presença de células foliculares. Os complexos cumulus-oócitos (CCOs) foram cultivados por 22 h na presença de células foliculares e Ang II, IGF-I ou Ins. Nesse…

Advisors/Committee Members: Paulo Bayard Dias Goncalves, Alceu Mezzalira.

Subjects/Keywords: bovino; oócito; maturação; angiotensina II; IGF-I; MEDICINA VETERINARIA; bovine; oocyte; maturation; angiotensin II; IGF-I

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APA (6^th Edition):

Stefanello, J. R. (2005). ANGIOTENSINA II E SUA ASSOCIAÇÃO COM FATOR-I DE CRESCIMENTO SEMELHANTE À INSULINA, INSULINA E CÉLULAS FOLICULARES NA MATURAÇÃO DE OÓCITOS BOVINOS E CONSEQÜENTE DESENVOLVIMENTO EMBRIONÁRIO. (Thesis). Universidade Federal de Santa Maria. Retrieved from http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=317

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Stefanello, Jerônimo Rubert. “ANGIOTENSINA II E SUA ASSOCIAÇÃO COM FATOR-I DE CRESCIMENTO SEMELHANTE À INSULINA, INSULINA E CÉLULAS FOLICULARES NA MATURAÇÃO DE OÓCITOS BOVINOS E CONSEQÜENTE DESENVOLVIMENTO EMBRIONÁRIO.” 2005. Thesis, Universidade Federal de Santa Maria. Accessed January 22, 2021. http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=317.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Stefanello, Jerônimo Rubert. “ANGIOTENSINA II E SUA ASSOCIAÇÃO COM FATOR-I DE CRESCIMENTO SEMELHANTE À INSULINA, INSULINA E CÉLULAS FOLICULARES NA MATURAÇÃO DE OÓCITOS BOVINOS E CONSEQÜENTE DESENVOLVIMENTO EMBRIONÁRIO.” 2005. Web. 22 Jan 2021.

Vancouver:

Stefanello JR. ANGIOTENSINA II E SUA ASSOCIAÇÃO COM FATOR-I DE CRESCIMENTO SEMELHANTE À INSULINA, INSULINA E CÉLULAS FOLICULARES NA MATURAÇÃO DE OÓCITOS BOVINOS E CONSEQÜENTE DESENVOLVIMENTO EMBRIONÁRIO. [Internet] [Thesis]. Universidade Federal de Santa Maria; 2005. [cited 2021 Jan 22]. Available from: http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=317.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Stefanello JR. ANGIOTENSINA II E SUA ASSOCIAÇÃO COM FATOR-I DE CRESCIMENTO SEMELHANTE À INSULINA, INSULINA E CÉLULAS FOLICULARES NA MATURAÇÃO DE OÓCITOS BOVINOS E CONSEQÜENTE DESENVOLVIMENTO EMBRIONÁRIO. [Thesis]. Universidade Federal de Santa Maria; 2005. Available from: http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=317

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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