subject:(bovine granulosa cells)

1. F. Pizzo. IN VITRO TOXICOLOGICAL EFFECTS OF FUSARIUM MYCOTOXINS ON BOVINE GRANULOSA CELLS.

Degree: 2015, Università degli Studi di Milano

► Information on the potential effects of fusariotoxins on reproductive system in ruminants are few. Therefore, the purpose of this study is to evaluate if DON… (more)

Subjects/Keywords: mycotoxins; granulosa cells; bovine; zearalenone; deoxynivalenol; steroidogenesis; Settore VET/07 - Farmacologia e Tossicologia Veterinaria

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Pizzo, F. (2015). IN VITRO TOXICOLOGICAL EFFECTS OF FUSARIUM MYCOTOXINS ON BOVINE GRANULOSA CELLS. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/258584

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Pizzo, F.. “IN VITRO TOXICOLOGICAL EFFECTS OF FUSARIUM MYCOTOXINS ON BOVINE GRANULOSA CELLS.” 2015. Thesis, Università degli Studi di Milano. Accessed March 06, 2021. http://hdl.handle.net/2434/258584.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Pizzo, F.. “IN VITRO TOXICOLOGICAL EFFECTS OF FUSARIUM MYCOTOXINS ON BOVINE GRANULOSA CELLS.” 2015. Web. 06 Mar 2021.

Vancouver:

Pizzo F. IN VITRO TOXICOLOGICAL EFFECTS OF FUSARIUM MYCOTOXINS ON BOVINE GRANULOSA CELLS. [Internet] [Thesis]. Università degli Studi di Milano; 2015. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/2434/258584.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Pizzo F. IN VITRO TOXICOLOGICAL EFFECTS OF FUSARIUM MYCOTOXINS ON BOVINE GRANULOSA CELLS. [Thesis]. Università degli Studi di Milano; 2015. Available from: http://hdl.handle.net/2434/258584

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Massey University

2. Li, Dongxing. Oxygen consumption of bovine granulosa cells in vitro.

Degree: M. Eng., Biotechnology, 2012, Massey University

URL: http://hdl.handle.net/10179/3986

► The oxygen consumption rate of granulosa cells is considered to be a key determinant of oocyte oxygenation in follicles. The oxygen status of the oocyte… (more)

▼ The oxygen consumption rate of granulosa cells is considered to be a key determinant of oocyte oxygenation in follicles. The oxygen status of the oocyte potentially dictates its developmental competence. However, quantitative information on the oxygen consumption rate of granulosa cells in literature is scarce. This limitation has hindered further investigation into the oocyte oxygenation, which could potentially be used as an indicator for selecting high quality oocytes for producing high quality embryos. This could ultimately contribute to improvement of the success rates of human In-Vitro Fertilisation. In light of this issue, this work developed a method for measuring the oxygen consumption rate of granulosa cells in vitro. This included techniques related to granulosa cell harvest from cows, suspending/culturing granulosa cells in culture medium and development of a competent respirometer. Each measurement run on the oxygen consumption rate of granulosa cells was conducted by suspending the granulosa cell culture in the respirometer, in which an optical-based oxygen sensor probe was employed to continuously monitor the oxygen partial pressure change in the cell suspension. Five separate sets of respirometer data were collected and used to calculate the oxygen consumption rate, giving a range of 2.1 to 3.3 x 10-16mol.cell-1.s-1/0.16 to 0.25mol.m-3.s-1. These rates were comparable with but higher than other animal cell oxygen consumption rates reported by the literature. They were approximately 5 times higher than the oxygen consumption rate of granulosa cells harvested from sheep (Gosden & Byatt-Smith 1986). The implications of the measured oxygen consumption rate were then examined in the context of oxygen transport in large bovine preantral follicles via an existing mathematical model. The resulting predicted oxygen profiles in large bovine follicles were consistent with the study of Redding et al. (2007), which showed that as a preantral follicle grew the oxygen transport across the follicle was increasingly strained, resulting in subsequent decrease in oocyte oxygenation. By applying the bovine specific parameter estimates to the model, this work predicted that the largest follicle radius for the oxygen transport in bovine preantral follicle was 134μm, beyond which the oxygen could not reach oocyte. Since the experimentally reported sizes of the large bovine preantral follicles ranged of 58 to 145μm in radius (see Section 7.1.2), this work proposed that the oxygen transport was capable of oxygenating the oocytes in all but the largest preantral follicles. If bovine preantral follicles were to grow larger than they are experimentally observed to do so, all oocytes contained within such follicles would be in a hypoxic state. This is a result consistent with other work. Furthermore, based on the use of bovine and ovine specific parameter estimates in the model, this work found that oxygen transport in follicles was likely to be the result of a unique combination of parameters for a particular species.…

Subjects/Keywords: Oxygen consumption; Granulosa cells; Oocytes; Oxygen consumption measurement; Bovine follicles; Oocyte oxygenation; Preantral follicles

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Li, D. (2012). Oxygen consumption of bovine granulosa cells in vitro. (Masters Thesis). Massey University. Retrieved from http://hdl.handle.net/10179/3986

Chicago Manual of Style (16^th Edition):

Li, Dongxing. “Oxygen consumption of bovine granulosa cells in vitro.” 2012. Masters Thesis, Massey University. Accessed March 06, 2021. http://hdl.handle.net/10179/3986.

MLA Handbook (7^th Edition):

Li, Dongxing. “Oxygen consumption of bovine granulosa cells in vitro.” 2012. Web. 06 Mar 2021.

Vancouver:

Li D. Oxygen consumption of bovine granulosa cells in vitro. [Internet] [Masters thesis]. Massey University; 2012. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/10179/3986.

Council of Science Editors:

Li D. Oxygen consumption of bovine granulosa cells in vitro. [Masters Thesis]. Massey University; 2012. Available from: http://hdl.handle.net/10179/3986

3. L. Terzaghi. ROLE OF PROGESTERONE RECEPTOR MEMBRANE COMPONENT 1 (PGRMC1) IN CONTROLLING GERMINAL AND SOMATIC CELL DIVISION AND FUNCTION.

Degree: 2018, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/550339

► La sub-fertilità rappresenta uno dei principali problemi che l'industria lattiero-casearia si trova ad affrontare attualmente. Si tratta di un problema multifattoriale che dipende da diversi… (more)

▼ La sub-fertilità rappresenta uno dei principali problemi che l'industria lattiero-casearia si trova ad affrontare attualmente. Si tratta di un problema multifattoriale che dipende da diversi aspetti della funzione riproduttiva. La qualità dell'ovocita, determinata durante la follicologenesi, è sicuramente cruciale per ottenere risultati riproduttivi ottimali. Per migliorare la fertilità femminile nel bovino è fondamentale definire i fattori e i meccanismi che determinano la qualità del gamete femminile, in quanto il declino della funzione riproduttiva in questa specie è dovuto principalmente alla scarsa qualità degli ovociti. In particolare, è importante conoscere quali proteine regolano la meiosi, la fecondabilità e lo sviluppo embrionale dell'ovocita. In questo progetto, abbiamo considerato principalmente il ruolo del Progesterone Receptor Membrane Component-1 (PGRMC1) sia nell'ovocita che nel compartimento follicolare, considerato che il dialogo tra compartimento germinale e compartimento somatico è uno dei fattori chiave coinvolti nell'acquisizione della competenza allo sviluppo del gamete femminile. Il PGRMC1 è una proteina multifunzionale ed altamente conservata in specie anche filogeneticamente distanti. Nei mammiferi è espressa in diversi sistemi, compresi gli organi riproduttivi, e localizza in diversi compartimenti subcellulari. Tuttavia, il suo ruolo e il preciso meccanismo d'azione in ogni sistema e/o compartimento subcellulare non sono ancora del tutto conosciuti. La parte più considerevole di questo progetto di dottorato riguarda gli studi effettuati sul ruolo del PGRMC1 nella divisione cellulare, data la sua localizzazione a livello di fuso mitotico e meiotico. Abbiamo dimostrato che la deplezione del PGRMC1 determina difetti nella divisione cellulare e la sua interazione con l'Aurora chinasi B (AURKB) indica che potrebbe svolgere la sua azione durante la citodieresi, l'ultima fase della divisione. Durante la maturazione meiotica, abbiamo valutato anche il suo ruolo nel mediare l'azione del progesterone (P4) confrontando il suo effetto con quello del recettore nucleare del progesterone (nPGR). L'inibizione di entrambi i tipi di recettore determina lo stesso effetto sull'organizzazione della piastra metafasica e sulla competenza allo sviluppo dell'ovocita, ma in fasi differenti. Inoltre, abbiamo testato l'ipotesi che il PGRMC1 possa modulare la funzione del nucleolo. Studi di immunofluorescenza hanno confermato la presenza del PGRMC1 nel nucleolo di cellule della granulosa (bGC) e negli ovociti di bovino, nonchè la colocalizzazione con la nucleolina, la proteina nucleolare più abbondante che svolge importanti funzioni in questo compartimento subcellulare. Inoltre, la down-regolazione del PGRMC1 determina uno spostamento della nucleolina dal nucleolo al nucleoplasma suggerendo un'associazione funzionale tra queste due proteine. Questa interazione è probabilmente mediata dalla presenza di ulteriori molecole in quanto successivi esperimenti di "in-situ proximity ligation" mostrano che le due… Advisors/Committee Members: tutor: V. Lodde, cotutor: A. M. Luciano, coordinatore: F. Gandolfi, LODDE, VALENTINA, GANDOLFI, FULVIO.

Subjects/Keywords: Progesterone Receptor Membrane Component 1; PGRMC1; fertility; reproduction; cell division; meiosis; mitosis; bovine granulosa cells; bovine oocyte; nucleolin; Canine Mammary tumors; progesterone; Settore VET/01 - Anatomia degli Animali Domestici; Settore VET/02 - Fisiologia Veterinaria; Settore BIO/06 - Anatomia Comparata e Citologia; Settore BIO/09 - Fisiologia; Settore BIO/11 - Biologia Molecolare; Settore BIO/17 - Istologia

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Terzaghi, L. (2018). ROLE OF PROGESTERONE RECEPTOR MEMBRANE COMPONENT 1 (PGRMC1) IN CONTROLLING GERMINAL AND SOMATIC CELL DIVISION AND FUNCTION. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/550339

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Terzaghi, L.. “ROLE OF PROGESTERONE RECEPTOR MEMBRANE COMPONENT 1 (PGRMC1) IN CONTROLLING GERMINAL AND SOMATIC CELL DIVISION AND FUNCTION.” 2018. Thesis, Università degli Studi di Milano. Accessed March 06, 2021. http://hdl.handle.net/2434/550339.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Terzaghi, L.. “ROLE OF PROGESTERONE RECEPTOR MEMBRANE COMPONENT 1 (PGRMC1) IN CONTROLLING GERMINAL AND SOMATIC CELL DIVISION AND FUNCTION.” 2018. Web. 06 Mar 2021.

Vancouver:

Terzaghi L. ROLE OF PROGESTERONE RECEPTOR MEMBRANE COMPONENT 1 (PGRMC1) IN CONTROLLING GERMINAL AND SOMATIC CELL DIVISION AND FUNCTION. [Internet] [Thesis]. Università degli Studi di Milano; 2018. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/2434/550339.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Terzaghi L. ROLE OF PROGESTERONE RECEPTOR MEMBRANE COMPONENT 1 (PGRMC1) IN CONTROLLING GERMINAL AND SOMATIC CELL DIVISION AND FUNCTION. [Thesis]. Università degli Studi di Milano; 2018. Available from: http://hdl.handle.net/2434/550339

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

4. Khan, Muhammad. Effect of Maternal Age on Transcriptome of Granulosa Cells from Bovine Dominant Follicles.

Degree: 2014, University of Saskatchewan

URL: http://hdl.handle.net/10388/ETD-2014-01-1364

► Advanced maternal age has been shown to influence follicular and luteal dynamics in bovine ovary resulting in reduced fertility. The overall objective of the four… (more)

▼ Advanced maternal age has been shown to influence follicular and luteal dynamics in bovine ovary resulting in reduced fertility. The overall objective of the four studies presented in this thesis is to identify the maternal age-associated transcriptional changes in granulosa cells of the dominant follicles during follicle development. In the first study, mRNA expression levels of housekeeping genes were measured by real–time quantitative PCR (RT-qPCR) in granulosa cells of dominant follicles and FSH-stimulated follicles to select and validate suitable reference genes for relative gene expression analyses during maternal and follicular aging. Stability of six reference genes (GAPDH, ACTB, EIF2B2, UBE2D2, SF3A1 and RNF20) was analyzed using GeNorm, DeltaCT and NormFinder programs and comprehensive ranking order was determined based on these programs. Geometric mean of multiple genes (UBE2D2, EIF2B2, GAPDH and SF3A1) was more appropriate reference control than individual genes for the comparison of relative gene expression among dominant and FSH-stimulated follicles during maternal and/or follicular aging studies. In the second study, maternal age-associated changes in the transcriptome of granulosa cells recovered at the time of selection of the dominant follicle from aged (n=3) and young cows (n=3) were determined by EmbryoGENE bovine oligo-microarrays (EMBV3, Agilent Technology). The mRNA expression of five transcripts (CYP19A1, PCNA, GJA1, TPM2, and VNN1) was confirmed in a different set of granulosa cell samples by RT-qPCR to validate microarray data. A total of 169 genes/isoforms were differentially expressed (≥ 2-fold-change; P ≤ 0.05) in aged cows vs. young cows. These transcripts revealed inefficient 1) control of gonadotropins, and gonadotropin-induced changes in the cytoskeleton and extracellular matrix, 2) lipid metabolism and steroidogenesis 3) cell proliferation, cell cycle control and intercellular communication, and 4) higher oxidative stress responses in aged cows vs. young cows. In the third study, changes in the transcriptome of granulosa cells of the preovulatory follicle 24 h after LH treatment from aged (n= 3) and young (n=3) were determined. A total of 1340 genes were expressed differentially (≥ 2-fold change; P ≤ 0.05) in aged cows vs. young cows. The mRNA expression of five transcripts (RGS2, PTGS2, TNFAIP6, VNN1, NR5A2 and GADD45B) was confirmed in a different set of granulosa cell samples to validate microarray data. These transcripts were related to delayed 1) response to LH treatment 2) cellular differentiation and luteinization and 3) progesterone synthesis. Intra-follicle levels of progesterone were lower (P < 0.05) in aged cows compared to young and mid-aged cows. The fourth study compared the aged-associated changes in the transcriptome of granulosa cells during follicle development from the time of dominant follicle selection to preovulatory stage (24 h after LH). In comparison to young cows, aged cows expressed fewer differentially expressed genes/isoforms (1206 vs. 2260, respectively)… Advisors/Committee Members: Singh, Jaswant, Adams, Gregg P., Sirard, Marc A., Misra, Vikram, Anzar, Muhammad.

Subjects/Keywords: Maternal age; Granulosa cells; Dominant follicle at selection; Preovulatory follicle; Reference genes; Microarrays; RT-qPCR; Bovine; Luteinizing hormone; Ovariectomy; Follicular aspiration; Transcriptome analysis; Ingenuity Pathway Analysis; Upstream regulators; Estradiol; Progesterone; Follicular fluid; Ultrasonography; Ovulation

…68 CHAPTER 4: MATERNAL AGE RELATED CHANGES IN TRANSCRIPTOME OF BOVINE GRANULOSA CELLS OF… …95 CHAPTER 5: MATERNAL AGE RELATED CHANGES IN TRANSCRIPTOME OF BOVINE GRANULOSA CELLS OF… …188 8.3 Maternal age related changes in transcriptome of bovine granulosa cells of… …GRANULOSA CELLS....................................... 50 3.1 3.2 3.2.1 3.2.2 3.2.3 3.2.4 3.2.5… …53 Collection of Granulosa cells…

11 more images…

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Khan, M. (2014). Effect of Maternal Age on Transcriptome of Granulosa Cells from Bovine Dominant Follicles. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/ETD-2014-01-1364

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Khan, Muhammad. “Effect of Maternal Age on Transcriptome of Granulosa Cells from Bovine Dominant Follicles.” 2014. Thesis, University of Saskatchewan. Accessed March 06, 2021. http://hdl.handle.net/10388/ETD-2014-01-1364.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Khan, Muhammad. “Effect of Maternal Age on Transcriptome of Granulosa Cells from Bovine Dominant Follicles.” 2014. Web. 06 Mar 2021.

Vancouver:

Khan M. Effect of Maternal Age on Transcriptome of Granulosa Cells from Bovine Dominant Follicles. [Internet] [Thesis]. University of Saskatchewan; 2014. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/10388/ETD-2014-01-1364.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Khan M. Effect of Maternal Age on Transcriptome of Granulosa Cells from Bovine Dominant Follicles. [Thesis]. University of Saskatchewan; 2014. Available from: http://hdl.handle.net/10388/ETD-2014-01-1364

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

		in
And / Or
		in
And / Or
		in
And / Or
		in

Open Access Theses and Dissertations