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1.
F. Pizzo.
IN VITRO TOXICOLOGICAL EFFECTS OF FUSARIUM MYCOTOXINS ON BOVINE GRANULOSA CELLS.
Degree: 2015, Università degli Studi di Milano
URL: http://hdl.handle.net/2434/258584 
► Information on the potential effects of fusariotoxins on reproductive system in ruminants are few. Therefore, the purpose of this study is to evaluate if DON…
(more)
▼ Information on the potential effects of fusariotoxins on reproductive system in ruminants are few. Therefore, the purpose of this study is to evaluate if DON and ZEA major hydroxylated metabolites, α-zearalenol (α-Zol) and β-zearalenone (β-Zol), alone or in combination, can impair
bovine reproductive activity via affecting cell proliferation, estradiol (E2) and progesterone (P4) production and gene expression (CYP11A1 and CYP19A1 mRNA abundance) of primary
granulosa cells (GC). GC were obtained aspirating aseptically small (1 to 5 mm) (SMGC) and large (8-22 mm) (LGGC) ovarian follicles. GC were cultured for 2 days in medium containing 10% fetal
bovine serum followed by 1 or 2 days in serum free medium without (control) or with added treatments. At the end of the experiments, numbers of GC were determined using a Coulter counter, the concentration of steroid hormones was evaluated via radioimmunoassay and RNA analysis was performed. In SMGC cell proliferation was negatively affected after exposure to β-Zol at 31 µM and after exposure to α-Zol (3.1 µM) alone and combined with DON (3.3 µM). Steroidogenesis was differently affected by tested mycotoxins: DON and α-Zol had inhibitory effects while β-Zol at high concentration (31 µM) stimulated steroid production. DON (3.3 µM) and β-Zol (31 µM) increased GC CYP19A1 mRNA abundance. CYP11A1 mRNA abundance was stimulated by DON, alone and combined with α-Zol and β-Zol, whereas was inhibited by β-Zol alone. In LGGC, α-Zol alone and in combination with DON, increased cell proliferation whereas DON strongly inhibited P4 and E2 production mainly at high doses (3.3 µM). In conclusion these results demonstrated that DON and ZEA metabolites may affect in vitro
bovine GC function and also provides information on the interaction between DON and ZEA metabolites.
Advisors/Committee Members: tutor: F. Caloni, coordinator: G. Savoini, CALONI, FRANCESCA, SAVOINI, GIOVANNI.
Subjects/Keywords: mycotoxins; granulosa cells; bovine; zearalenone; deoxynivalenol; steroidogenesis; Settore VET/07 - Farmacologia e Tossicologia Veterinaria
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APA (6th Edition):
Pizzo, F. (2015). IN VITRO TOXICOLOGICAL EFFECTS OF FUSARIUM MYCOTOXINS ON BOVINE GRANULOSA CELLS. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/258584
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Pizzo, F.. “IN VITRO TOXICOLOGICAL EFFECTS OF FUSARIUM MYCOTOXINS ON BOVINE GRANULOSA CELLS.” 2015. Thesis, Università degli Studi di Milano. Accessed January 22, 2021.
http://hdl.handle.net/2434/258584.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Pizzo, F.. “IN VITRO TOXICOLOGICAL EFFECTS OF FUSARIUM MYCOTOXINS ON BOVINE GRANULOSA CELLS.” 2015. Web. 22 Jan 2021.
Vancouver:
Pizzo F. IN VITRO TOXICOLOGICAL EFFECTS OF FUSARIUM MYCOTOXINS ON BOVINE GRANULOSA CELLS. [Internet] [Thesis]. Università degli Studi di Milano; 2015. [cited 2021 Jan 22].
Available from: http://hdl.handle.net/2434/258584.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Pizzo F. IN VITRO TOXICOLOGICAL EFFECTS OF FUSARIUM MYCOTOXINS ON BOVINE GRANULOSA CELLS. [Thesis]. Università degli Studi di Milano; 2015. Available from: http://hdl.handle.net/2434/258584
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Massey University
2.
Li, Dongxing.
Oxygen consumption of bovine granulosa cells in vitro.
Degree: M. Eng., Biotechnology, 2012, Massey University
URL: http://hdl.handle.net/10179/3986
► The oxygen consumption rate of granulosa cells is considered to be a key determinant of oocyte oxygenation in follicles. The oxygen status of the oocyte…
(more)
▼ The oxygen consumption rate of granulosa cells is considered to be a key determinant of oocyte oxygenation in follicles. The oxygen status of the oocyte potentially dictates its developmental competence. However, quantitative information on the oxygen consumption rate of granulosa cells in literature is scarce. This limitation has hindered further investigation into the oocyte oxygenation, which could potentially be used as an indicator for selecting high quality oocytes for producing high quality embryos. This could ultimately contribute to improvement of the success rates of human In-Vitro Fertilisation.
In light of this issue, this work developed a method for measuring the oxygen consumption rate of granulosa cells in vitro. This included techniques related to granulosa cell harvest from cows, suspending/culturing granulosa cells in culture medium and development of a competent respirometer. Each measurement run on the oxygen consumption rate of granulosa cells was conducted by suspending the granulosa cell culture in the respirometer, in which an optical-based oxygen sensor probe was employed to continuously monitor the oxygen partial pressure change in the cell suspension.
Five separate sets of respirometer data were collected and used to calculate the oxygen consumption rate, giving a range of 2.1 to 3.3 x 10-16mol.cell-1.s-1/0.16 to 0.25mol.m-3.s-1. These rates were comparable with but higher than other animal cell oxygen consumption rates reported by the literature. They were approximately 5 times higher than the oxygen consumption rate of granulosa cells harvested from sheep (Gosden & Byatt-Smith 1986).
The implications of the measured oxygen consumption rate were then examined in the context of oxygen transport in large bovine preantral follicles via an existing mathematical model. The resulting predicted oxygen profiles in large bovine follicles were consistent with the study of Redding et al. (2007), which showed that as a preantral follicle grew the oxygen transport across the follicle was increasingly strained, resulting in subsequent decrease in oocyte oxygenation. By applying the bovine specific parameter estimates to the model, this work predicted that the largest follicle radius for the oxygen transport in bovine preantral follicle was 134μm, beyond which the oxygen could not reach oocyte. Since the experimentally reported sizes of the large bovine preantral follicles ranged of 58 to 145μm in radius (see Section 7.1.2), this work proposed that the oxygen transport was capable of oxygenating the oocytes in all but the largest preantral follicles. If bovine preantral follicles were to grow larger than they are experimentally observed to do so, all oocytes contained within such follicles would be in a hypoxic state. This is a result consistent with other work.
Furthermore, based on the use of bovine and ovine specific parameter estimates in the model, this work found that oxygen transport in follicles was likely to be the result of a unique combination of parameters for a particular species.…
Subjects/Keywords: Oxygen consumption;
Granulosa cells;
Oocytes;
Oxygen consumption measurement;
Bovine follicles;
Oocyte oxygenation;
Preantral follicles
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APA (6th Edition):
Li, D. (2012). Oxygen consumption of bovine granulosa cells in vitro. (Masters Thesis). Massey University. Retrieved from http://hdl.handle.net/10179/3986
Chicago Manual of Style (16th Edition):
Li, Dongxing. “Oxygen consumption of bovine granulosa cells in vitro.” 2012. Masters Thesis, Massey University. Accessed January 22, 2021.
http://hdl.handle.net/10179/3986.
MLA Handbook (7th Edition):
Li, Dongxing. “Oxygen consumption of bovine granulosa cells in vitro.” 2012. Web. 22 Jan 2021.
Vancouver:
Li D. Oxygen consumption of bovine granulosa cells in vitro. [Internet] [Masters thesis]. Massey University; 2012. [cited 2021 Jan 22].
Available from: http://hdl.handle.net/10179/3986.
Council of Science Editors:
Li D. Oxygen consumption of bovine granulosa cells in vitro. [Masters Thesis]. Massey University; 2012. Available from: http://hdl.handle.net/10179/3986
University of Edinburgh
3.
Doyle, Lynsey Kerr.
Role of vascular endothelial growth factor (VEGF) in granulosa cell function : involvement of heterotrimeric G-protein signalling pathways.
Degree: PhD, 2009, University of Edinburgh
URL: http://hdl.handle.net/1842/4297
► Vascular Endothelial Growth Factor (VEGF) has been shown to be an absolute requirement for ovarian follicle development. Although VEGF is commonly regarded primarily as an…
(more)
▼ Vascular Endothelial Growth Factor (VEGF) has been shown to be an absolute requirement for ovarian follicle development. Although VEGF is commonly regarded primarily as an angiogenic factor, granulosa cells are a major site of VEGF synthesis in the follicle and they express VEGF receptors (VEGFR1 and VEGFR2). Further, the development of the dominant follicle is characterised by a substantial increase in granulosa cell expression of VEGF and its receptors. In spite of this, potential non-angiogenic effects of VEGF in these follicles have not been elucidated. The objective of the three studies described in this thesis was to use an in vitro bovine granulosa cell model to investigate the roles of VEGF during development of the dominant follicle. In addition, in light of evidence in other cell types, potential interactions between VEGF signalling and heterotrimeric protein signalling in these follicles were also investigated. In the first study, granulosa cells were obtained from healthy follicles with diameters of 4 to 8 mm (corresponding to just before the selection of a dominant follicle during a follicular wave) or 9 to 14 mm (encompassing all developmental stages of a dominant follicle) and exposed to a range of VEGF concentrations (1 to 100 ng/ml) encompassing concentrations found naturally in bovine dominant follicles. VEGF at 1 ng/ml, but not at higher concentrations (P > 0.1), induced significant proliferation of bovine granulosa cells from 4 to 8 mm follicles (P = 0.024) and increased the proliferative response of these cells to FSH (P = 0.045). VEGF also induced a dose-dependent increase in ERK1/2 activation by granulosa cells from 4 to 8 mm follicles (P < 0.03) but did not have any effect on expression of the steroidogenic enzyme, CYP11A1, by these cells (P > 0.1). VEGF, at a dose of 1 ng/ml (P = 0.003), but not at higher doses (P > 0.1), induced an increase in COX-2 expression by granulosa cells from 9 to 14 mm follicles. In addition, LH stimulation of both ERK phosphorylation (P < 0.05) and COX-2 expression (P < 0.05) in granulosa cells from 9 to 14 mm follicles were prevented (P > 0.1) by specific inhibition of VEGFR2, indicating that VEGF may mediate COX-2 responses to LH in these cells. The second study sought to examine the expression of heterotrimeric G-protein á subunits and PLCâ isoforms by real-time PCR and westen blotting in bovine granulosa cells throughout follicle development to identify specific molecular components of heterotrimeric G-protein pathways that may functionally interact with intracellular VEGF signals. Results showed that GNAS, GNA11 and GNAI2 were all expressed at significantly (P < 0.05) higher levels in granulosa cells of pre-ovulatorysize follicles (10.0 to 13.9 mm) than in cells from smaller follicles (2.0 to 5.9 mm and 6.0 to 9.9 mm). In addition, all PLCB isoforms except PLCB2 were expressed in bovine granulosa cells with PLCB3 being more abundant than PLCB1 and -4. Levels of PLCB3 in granulosa cells from pre-ovulatory-size follicles were much higher (>16-fold; P < 0.005)…
Subjects/Keywords: 636.089; vascular endothelial growth factor; VEGF; ovarian follicle development; granulosa cells; bovine; cattle
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APA ·
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APA (6th Edition):
Doyle, L. K. (2009). Role of vascular endothelial growth factor (VEGF) in granulosa cell function : involvement of heterotrimeric G-protein signalling pathways. (Doctoral Dissertation). University of Edinburgh. Retrieved from http://hdl.handle.net/1842/4297
Chicago Manual of Style (16th Edition):
Doyle, Lynsey Kerr. “Role of vascular endothelial growth factor (VEGF) in granulosa cell function : involvement of heterotrimeric G-protein signalling pathways.” 2009. Doctoral Dissertation, University of Edinburgh. Accessed January 22, 2021.
http://hdl.handle.net/1842/4297.
MLA Handbook (7th Edition):
Doyle, Lynsey Kerr. “Role of vascular endothelial growth factor (VEGF) in granulosa cell function : involvement of heterotrimeric G-protein signalling pathways.” 2009. Web. 22 Jan 2021.
Vancouver:
Doyle LK. Role of vascular endothelial growth factor (VEGF) in granulosa cell function : involvement of heterotrimeric G-protein signalling pathways. [Internet] [Doctoral dissertation]. University of Edinburgh; 2009. [cited 2021 Jan 22].
Available from: http://hdl.handle.net/1842/4297.
Council of Science Editors:
Doyle LK. Role of vascular endothelial growth factor (VEGF) in granulosa cell function : involvement of heterotrimeric G-protein signalling pathways. [Doctoral Dissertation]. University of Edinburgh; 2009. Available from: http://hdl.handle.net/1842/4297
4.
L. Terzaghi.
ROLE OF PROGESTERONE RECEPTOR MEMBRANE COMPONENT 1 (PGRMC1) IN CONTROLLING GERMINAL AND SOMATIC CELL DIVISION AND FUNCTION.
Degree: 2018, Università degli Studi di Milano
URL: http://hdl.handle.net/2434/550339
► La sub-fertilità rappresenta uno dei principali problemi che l'industria lattiero-casearia si trova ad affrontare attualmente. Si tratta di un problema multifattoriale che dipende da diversi…
(more)
▼ La sub-fertilità rappresenta uno dei principali problemi che l'industria lattiero-casearia si trova ad affrontare attualmente. Si tratta di un problema multifattoriale che dipende da diversi aspetti della funzione riproduttiva. La qualità dell'ovocita, determinata durante la follicologenesi, è sicuramente cruciale per ottenere risultati riproduttivi ottimali. Per migliorare la fertilità femminile nel bovino è fondamentale definire i fattori e i meccanismi che determinano la qualità del gamete femminile, in quanto il declino della funzione riproduttiva in questa specie è dovuto principalmente alla scarsa qualità degli ovociti. In particolare, è importante conoscere quali proteine regolano la meiosi, la fecondabilità e lo sviluppo embrionale dell'ovocita. In questo progetto, abbiamo considerato principalmente il ruolo del Progesterone Receptor Membrane Component-1 (PGRMC1) sia nell'ovocita che nel compartimento follicolare, considerato che il dialogo tra compartimento germinale e compartimento somatico è uno dei fattori chiave coinvolti nell'acquisizione della competenza allo sviluppo del gamete femminile.
Il PGRMC1 è una proteina multifunzionale ed altamente conservata in specie anche filogeneticamente distanti. Nei mammiferi è espressa in diversi sistemi, compresi gli organi riproduttivi, e localizza in diversi compartimenti subcellulari. Tuttavia, il suo ruolo e il preciso meccanismo d'azione in ogni sistema e/o compartimento subcellulare non sono ancora del tutto conosciuti.
La parte più considerevole di questo progetto di dottorato riguarda gli studi effettuati sul ruolo del PGRMC1 nella divisione cellulare, data la sua localizzazione a livello di fuso mitotico e meiotico. Abbiamo dimostrato che la deplezione del PGRMC1 determina difetti nella divisione cellulare e la sua interazione con l'Aurora chinasi B (AURKB) indica che potrebbe svolgere la sua azione durante la citodieresi, l'ultima fase della divisione. Durante la maturazione meiotica, abbiamo valutato anche il suo ruolo nel mediare l'azione del progesterone (P4) confrontando il suo effetto con quello del recettore nucleare del progesterone (nPGR). L'inibizione di entrambi i tipi di recettore determina lo stesso effetto sull'organizzazione della piastra metafasica e sulla competenza allo sviluppo dell'ovocita, ma in fasi differenti.
Inoltre, abbiamo testato l'ipotesi che il PGRMC1 possa modulare la funzione del nucleolo. Studi di immunofluorescenza hanno confermato la presenza del PGRMC1 nel nucleolo di cellule della
granulosa (bGC) e negli ovociti di bovino, nonchè la colocalizzazione con la nucleolina, la proteina nucleolare più abbondante che svolge importanti funzioni in questo compartimento
subcellulare. Inoltre, la down-regolazione del PGRMC1 determina uno spostamento della nucleolina dal nucleolo al nucleoplasma suggerendo un'associazione funzionale tra queste due proteine. Questa interazione è probabilmente mediata dalla presenza di ulteriori molecole in quanto successivi esperimenti di "in-situ proximity ligation" mostrano che le due…
Advisors/Committee Members: tutor: V. Lodde, cotutor: A. M. Luciano, coordinatore: F. Gandolfi, LODDE, VALENTINA, GANDOLFI, FULVIO.
Subjects/Keywords: Progesterone Receptor Membrane Component 1; PGRMC1; fertility; reproduction; cell division; meiosis; mitosis; bovine granulosa cells; bovine oocyte; nucleolin; Canine Mammary tumors; progesterone; Settore VET/01 - Anatomia degli Animali Domestici; Settore VET/02 - Fisiologia Veterinaria; Settore BIO/06 - Anatomia Comparata e Citologia; Settore BIO/09 - Fisiologia; Settore BIO/11 - Biologia Molecolare; Settore BIO/17 - Istologia
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APA (6th Edition):
Terzaghi, L. (2018). ROLE OF PROGESTERONE RECEPTOR MEMBRANE COMPONENT 1 (PGRMC1) IN CONTROLLING GERMINAL AND SOMATIC CELL DIVISION AND FUNCTION. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/550339
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Terzaghi, L.. “ROLE OF PROGESTERONE RECEPTOR MEMBRANE COMPONENT 1 (PGRMC1) IN CONTROLLING GERMINAL AND SOMATIC CELL DIVISION AND FUNCTION.” 2018. Thesis, Università degli Studi di Milano. Accessed January 22, 2021.
http://hdl.handle.net/2434/550339.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Terzaghi, L.. “ROLE OF PROGESTERONE RECEPTOR MEMBRANE COMPONENT 1 (PGRMC1) IN CONTROLLING GERMINAL AND SOMATIC CELL DIVISION AND FUNCTION.” 2018. Web. 22 Jan 2021.
Vancouver:
Terzaghi L. ROLE OF PROGESTERONE RECEPTOR MEMBRANE COMPONENT 1 (PGRMC1) IN CONTROLLING GERMINAL AND SOMATIC CELL DIVISION AND FUNCTION. [Internet] [Thesis]. Università degli Studi di Milano; 2018. [cited 2021 Jan 22].
Available from: http://hdl.handle.net/2434/550339.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Terzaghi L. ROLE OF PROGESTERONE RECEPTOR MEMBRANE COMPONENT 1 (PGRMC1) IN CONTROLLING GERMINAL AND SOMATIC CELL DIVISION AND FUNCTION. [Thesis]. Università degli Studi di Milano; 2018. Available from: http://hdl.handle.net/2434/550339
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
5.
Khan, Muhammad.
Effect of Maternal Age on Transcriptome of Granulosa Cells from Bovine Dominant Follicles.
Degree: 2014, University of Saskatchewan
URL: http://hdl.handle.net/10388/ETD-2014-01-1364
► Advanced maternal age has been shown to influence follicular and luteal dynamics in bovine ovary resulting in reduced fertility. The overall objective of the four…
(more)
▼ Advanced maternal age has been shown to influence follicular and luteal dynamics in
bovine ovary resulting in reduced fertility. The overall objective of the four studies presented in this thesis is to identify the maternal age-associated transcriptional changes in
granulosa cells of the dominant follicles during follicle development.
In the first study, mRNA expression levels of housekeeping genes were measured by real–time quantitative PCR (RT-qPCR) in
granulosa cells of dominant follicles and FSH-stimulated follicles to select and validate suitable reference genes for relative gene expression analyses during maternal and follicular aging. Stability of six reference genes (GAPDH, ACTB, EIF2B2, UBE2D2, SF3A1 and RNF20) was analyzed using GeNorm, DeltaCT and NormFinder programs and comprehensive ranking order was determined based on these programs. Geometric mean of multiple genes (UBE2D2, EIF2B2, GAPDH and SF3A1) was more appropriate reference control than individual genes for the comparison of relative gene expression among dominant and FSH-stimulated follicles during maternal and/or follicular aging studies.
In the second study, maternal age-associated changes in the transcriptome of
granulosa cells recovered at the time of selection of the dominant follicle from aged (n=3) and young cows (n=3) were determined by EmbryoGENE
bovine oligo-microarrays (EMBV3, Agilent Technology). The mRNA expression of five transcripts (CYP19A1, PCNA, GJA1, TPM2, and VNN1) was confirmed in a different set of
granulosa cell samples by RT-qPCR to validate microarray data. A total of 169 genes/isoforms were differentially expressed (≥ 2-fold-change; P ≤ 0.05) in aged cows vs. young cows. These transcripts revealed inefficient 1) control of gonadotropins, and gonadotropin-induced changes in the cytoskeleton and extracellular matrix, 2) lipid metabolism and steroidogenesis 3) cell proliferation, cell cycle control and intercellular communication, and 4) higher oxidative stress responses in aged cows vs. young cows.
In the third study, changes in the transcriptome of
granulosa cells of the preovulatory follicle 24 h after LH treatment from aged (n= 3) and young (n=3) were determined. A total of 1340 genes were expressed differentially (≥ 2-fold change; P ≤ 0.05) in aged cows vs. young cows. The mRNA expression of five transcripts (RGS2, PTGS2, TNFAIP6, VNN1, NR5A2 and GADD45B) was confirmed in a different set of
granulosa cell samples to validate microarray data. These transcripts were related to delayed 1) response to LH treatment 2) cellular differentiation and luteinization and 3) progesterone synthesis. Intra-follicle levels of progesterone were lower (P < 0.05) in aged cows compared to young and mid-aged cows.
The fourth study compared the aged-associated changes in the transcriptome of
granulosa cells during follicle development from the time of dominant follicle selection to preovulatory stage (24 h after LH). In comparison to young cows, aged cows expressed fewer differentially expressed genes/isoforms (1206 vs. 2260, respectively)…
Advisors/Committee Members: Singh, Jaswant, Adams, Gregg P., Sirard, Marc A., Misra, Vikram, Anzar, Muhammad.
Subjects/Keywords: Maternal age; Granulosa cells; Dominant follicle at selection; Preovulatory follicle; Reference genes; Microarrays; RT-qPCR; Bovine; Luteinizing hormone; Ovariectomy; Follicular aspiration; Transcriptome analysis; Ingenuity Pathway Analysis; Upstream regulators; Estradiol; Progesterone; Follicular fluid; Ultrasonography; Ovulation
…68
CHAPTER 4: MATERNAL AGE RELATED CHANGES IN TRANSCRIPTOME OF
BOVINE GRANULOSA CELLS OF… …95
CHAPTER 5: MATERNAL AGE RELATED CHANGES IN TRANSCRIPTOME OF
BOVINE GRANULOSA CELLS OF… …188
8.3
Maternal age related changes in transcriptome of bovine granulosa cells of… …GRANULOSA CELLS....................................... 50
3.1
3.2
3.2.1
3.2.2
3.2.3
3.2.4
3.2.5… …53
Collection of Granulosa cells…
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
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to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Khan, M. (2014). Effect of Maternal Age on Transcriptome of Granulosa Cells from Bovine Dominant Follicles. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/ETD-2014-01-1364
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Khan, Muhammad. “Effect of Maternal Age on Transcriptome of Granulosa Cells from Bovine Dominant Follicles.” 2014. Thesis, University of Saskatchewan. Accessed January 22, 2021.
http://hdl.handle.net/10388/ETD-2014-01-1364.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Khan, Muhammad. “Effect of Maternal Age on Transcriptome of Granulosa Cells from Bovine Dominant Follicles.” 2014. Web. 22 Jan 2021.
Vancouver:
Khan M. Effect of Maternal Age on Transcriptome of Granulosa Cells from Bovine Dominant Follicles. [Internet] [Thesis]. University of Saskatchewan; 2014. [cited 2021 Jan 22].
Available from: http://hdl.handle.net/10388/ETD-2014-01-1364.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Khan M. Effect of Maternal Age on Transcriptome of Granulosa Cells from Bovine Dominant Follicles. [Thesis]. University of Saskatchewan; 2014. Available from: http://hdl.handle.net/10388/ETD-2014-01-1364
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
. 
Open Access Theses and Dissertations
