subject:(bone morphogenetic proteins)

Rutgers University

1. Marmion, Robert. Function and regulation of wishful thinking, a BMP type II receptor.

Degree: PhD, Computational and Integrative Biology, 2016, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/49815/

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Development is controlled by a surprisingly small number of genetic pathways. One such pathway is called the bone morphogenetic protein (BMP) pathway, similar from flies… (more)

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Development is controlled by a surprisingly small number of genetic pathways. One such pathway is called the bone morphogenetic protein (BMP) pathway, similar from flies to humans. We used the common fruit fly, Drosophila melanogaster, to study the BMP pathway during Drosophila oogenesis, the formation of the egg. While the pathway is relatively simple, there exist combinations between the three different ligands, and four different receptors. My work focused largely on the two type II receptor, specifically on Wishful thinking (WIT). Much is known about the dynamic expression of the type I receptor during oogenesis, Thickveins. However, the pathway requires action of both type I and type II receptors. We found that WIT performs a necessary role during oogenesis and is regulated, indirectly, by BMP signaling. WIT is required for proper patterning of pathway target genes and necessary for proper formation of the eggshell. We also used a new technology, CRISPR/Cas9, to specifically remove the WIT locus from its endogenous location in the genome. This allowed for introduction of new alleles, including a tagged variant and conditional null, for future study of this gene. In the future, this may be applied to other genes in Drosophila oogenesis. Since gene patterning is crucial to development, we also worked in a collaborative effort to describe other complex gene patterns during Drosophila oogenesis. The Chorion proteins (CPs) are constituents of the eggshell and are expressed in a highly complex and dynamic fashion. We developed a system to computationally analyze gene pattering, dynamics, and conservation by utilizing a simple binary matrix. This matrix was then able to be used to predict the origin of a new domain in a related species of Drosophilid, the dorsal ridge of D. nebulosa and D. willistoni. This work served as a jumping point for multiple lab projects to study the patterning of this domain by another signaling pathway, the epidermal growth factor receptor (EGFR).

Advisors/Committee Members: Yakoby, Nir (chair), Lun, Desmond (co-chair), Nam, Jongmin (internal member), Padgett, Richard W. (outside member).

Subjects/Keywords: Bone morphogenetic proteins; Drosophila

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APA (6^th Edition):

Marmion, R. (2016). Function and regulation of wishful thinking, a BMP type II receptor. (Doctoral Dissertation). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/49815/

Chicago Manual of Style (16^th Edition):

Marmion, Robert. “Function and regulation of wishful thinking, a BMP type II receptor.” 2016. Doctoral Dissertation, Rutgers University. Accessed March 05, 2021. https://rucore.libraries.rutgers.edu/rutgers-lib/49815/.

MLA Handbook (7^th Edition):

Marmion, Robert. “Function and regulation of wishful thinking, a BMP type II receptor.” 2016. Web. 05 Mar 2021.

Vancouver:

Marmion R. Function and regulation of wishful thinking, a BMP type II receptor. [Internet] [Doctoral dissertation]. Rutgers University; 2016. [cited 2021 Mar 05]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/49815/.

Council of Science Editors:

Marmion R. Function and regulation of wishful thinking, a BMP type II receptor. [Doctoral Dissertation]. Rutgers University; 2016. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/49815/

University of Minnesota

2. Espe, Kelly Christine. Bone Morphogenetic Protein-2 (BMP2) upregulates osteoclast gene expression.

Degree: 2009, University of Minnesota

URL: http://purl.umn.edu/59781

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University of Minnesota M.S. thesis. November 2009. Major: Dentistry. Advisors:Dr. Brent Larson and Dr. John Beyer. 1 computer file (PDF); vi, 39 pages. Ill. (some… (more)

Subjects/Keywords: Bone Morphogenetic Proteins; Bone; Osteoclasts; Dentistry

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APA (6^th Edition):

Espe, K. C. (2009). Bone Morphogenetic Protein-2 (BMP2) upregulates osteoclast gene expression. (Masters Thesis). University of Minnesota. Retrieved from http://purl.umn.edu/59781

Chicago Manual of Style (16^th Edition):

Espe, Kelly Christine. “Bone Morphogenetic Protein-2 (BMP2) upregulates osteoclast gene expression.” 2009. Masters Thesis, University of Minnesota. Accessed March 05, 2021. http://purl.umn.edu/59781.

MLA Handbook (7^th Edition):

Espe, Kelly Christine. “Bone Morphogenetic Protein-2 (BMP2) upregulates osteoclast gene expression.” 2009. Web. 05 Mar 2021.

Vancouver:

Espe KC. Bone Morphogenetic Protein-2 (BMP2) upregulates osteoclast gene expression. [Internet] [Masters thesis]. University of Minnesota; 2009. [cited 2021 Mar 05]. Available from: http://purl.umn.edu/59781.

Council of Science Editors:

Espe KC. Bone Morphogenetic Protein-2 (BMP2) upregulates osteoclast gene expression. [Masters Thesis]. University of Minnesota; 2009. Available from: http://purl.umn.edu/59781

University of Illinois – Chicago

3. Ali, Saba. Elution Characteristics of Bone Morphogenetic Proteins from Demineralized Bone Matrix.

Degree: 2013, University of Illinois – Chicago

URL: http://hdl.handle.net/10027/10351

► Little is known of the elution characteristics of bone morphogenetic proteins (BMPs), proteins involved with the bone-forming mechanism, out of demineralized bone matrix (DBM).DBM is… (more)

▼ Little is known of the elution characteristics of bone morphogenetic proteins (BMPs), proteins involved with the bone-forming mechanism, out of demineralized bone matrix (DBM).DBM is a type of graft commonly used for in-vivo orthopedic surgical treatments to repair various bone abnormalities.Knowledge of the elution characteristics of BMPs out of DBM could improve the understanding of DBM function and help optimize graft design. Two sets of experiments were performed in this study; one examining the elution of BMP-7 out of bovine DBM for a period of three months, and the other examines the elution of BMPs-2, -4, and -7 out of human DBM for seven days.For the first set of experiments, the elution characteristics of BMPs out of bovine DBM were examined in-vitro using a variety of approaches as a probe to determine the association of BMPs with DBM over time.Once the DBM is placed into an aqueous environment, and as DBM hydration time increases, there is an unexpected increase in DBM BMP content as well; for example, more BMPs in the DBM can be accounted for a few days into DBM hydration versus zero hours of DBM hydration.Results support the idea that DBM hydration time impacts the complex association of BMPs with DBM, and that elution of BMPs from DBM in an aqueous environment occurs over a period of several weeks to months, which is longer than would be expected to be necessary to effect bone repair.The second set of experiments was the first to measure, and attempt to analyze the elution of human BMPs from a human DBM bone graft.The elution profiles of the human DBM were similar to the bovine DBM for the most part; however, some of the differences observed could be due to the additional carrier added to the human DBM which was not present in the bovine DBM.The elution profiles of BMPs -2, -4, and -7 were qualitatively similar to each other, differing mainly in magnitude which appeared to be related to the initial concentrations of these individual BMPs in DBM. This was the longest-term, most detailed study of the elution of BMPs from DBM that has ever been conducted which is relevant to an understanding of the osteoinductive process as well as basic bone biology.The results from this study may also be used to optimize the concentration of BMPs in a graft which can increase safety and cost benefits, and ultimately maximize DBM graft function. Advisors/Committee Members: Magin, Richard (advisor), Eddington, David (committee member), Pietrzak, William (committee member).

Subjects/Keywords: demineralized bone matrix; bone morphogenetic proteins

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APA (6^th Edition):

Ali, S. (2013). Elution Characteristics of Bone Morphogenetic Proteins from Demineralized Bone Matrix. (Thesis). University of Illinois – Chicago. Retrieved from http://hdl.handle.net/10027/10351

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ali, Saba. “Elution Characteristics of Bone Morphogenetic Proteins from Demineralized Bone Matrix.” 2013. Thesis, University of Illinois – Chicago. Accessed March 05, 2021. http://hdl.handle.net/10027/10351.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ali, Saba. “Elution Characteristics of Bone Morphogenetic Proteins from Demineralized Bone Matrix.” 2013. Web. 05 Mar 2021.

Vancouver:

Ali S. Elution Characteristics of Bone Morphogenetic Proteins from Demineralized Bone Matrix. [Internet] [Thesis]. University of Illinois – Chicago; 2013. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/10027/10351.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ali S. Elution Characteristics of Bone Morphogenetic Proteins from Demineralized Bone Matrix. [Thesis]. University of Illinois – Chicago; 2013. Available from: http://hdl.handle.net/10027/10351

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Otago

4. Trought, Robert Michael. The role of bone morphogenetic proteins in the development of the Xenopus laevis tail .

Degree: 2011, University of Otago

URL: http://hdl.handle.net/10523/1810

► This thesis looks at the role of bone morphogenetic proteins (BMPs) during tail development in Xenopus laevis. BMPs are a group of signalling molecules and… (more)

▼ This thesis looks at the role of bone morphogenetic proteins (BMPs) during tail development in Xenopus laevis. BMPs are a group of signalling molecules and their role was studied by inhibiting them from the onset of neurulation to the tailbud stage. A time period in which tissues differentiate and organs develop. Bone Morphogenetic Proteins have been well studied during early development and are involved in establishing the dorso-ventral axis through interactions with the antagonists; Chordin, Noggin and Follistatin. However, the removal of BMP activity results in embryonic death. In order to study the effects of BMP signalling in later development, a transgenic line of Xenopus that allows heat-shock inducible inhibition of BMPs was utilised. The transgene construct consists of the heatshock promoter HSP70, which drives expression of Noggin1, an inhibitor of the BMP signalling pathway. The presence of a post-anal tail is a defining characteristic of chordates. Previous studies have shown an involvement of BMP signaling in initiation of tail bud development. Results in this study demonstrated that continued BMP signaling, throughout neurulation until tail bud stages, was required for the proper development of Xenopus. The inhibition of BMPs during early neurulation in particular, resulted in gross deformation of the embryos. BMP signaling was found to be critical in proper formation of the heart, gastrointestinal tract, eye, proctodeum and the tail. The inhibition of BMPs at the onset of neurulation (stage 13) was sufficient to prevent tail formation in Xenopus embryos. This is likely to be the result of inhibition on the expression of XDelta-1 thought to be a critical component of tail bud initiation. BMP inhibition from stages 14 to 28 resulted in embryos with shortened tails. The extent of truncation declined as inhibition was delayed through these stages. The truncation of tails also coincided with a lack of differentiation of somites and a reduction in the staining of the Notch ligand XDelta-1 in the posterior wall of the tail bud. This suggests a role of BMPs in regulating Notch signaling, a pathway involved in differentiation of somites. A further role of BMPs signaling was identified in excluding the proctodeum from the posterior region of the embryo. Previously the incorporation of the proctodeum into the tail bud region has been described as an ectopic forked tail. Analysis utilising in-situ hybridisation enabled this structure to be correctly identified. This study has shown a continued role of BMPs in the development of the embryo throughout neurulation. Advisors/Committee Members: Beck, Caroline W (advisor).

Subjects/Keywords: Xenopus; BMPs; Tail; Bone Morphogenetic Proteins

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APA (6^th Edition):

Trought, R. M. (2011). The role of bone morphogenetic proteins in the development of the Xenopus laevis tail . (Masters Thesis). University of Otago. Retrieved from http://hdl.handle.net/10523/1810

Chicago Manual of Style (16^th Edition):

Trought, Robert Michael. “The role of bone morphogenetic proteins in the development of the Xenopus laevis tail .” 2011. Masters Thesis, University of Otago. Accessed March 05, 2021. http://hdl.handle.net/10523/1810.

MLA Handbook (7^th Edition):

Trought, Robert Michael. “The role of bone morphogenetic proteins in the development of the Xenopus laevis tail .” 2011. Web. 05 Mar 2021.

Vancouver:

Trought RM. The role of bone morphogenetic proteins in the development of the Xenopus laevis tail . [Internet] [Masters thesis]. University of Otago; 2011. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/10523/1810.

Council of Science Editors:

Trought RM. The role of bone morphogenetic proteins in the development of the Xenopus laevis tail . [Masters Thesis]. University of Otago; 2011. Available from: http://hdl.handle.net/10523/1810

University of Toronto

5. Prichert, Marina. The Effect of Zoledronate Pretreatment on BMP Induced Bone Formation in Mice.

Degree: 2011, University of Toronto

URL: http://hdl.handle.net/1807/31392

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Recombinant human bone morphogenetic proteins (rhBMPs) are increasingly used for reconstructing bony defects, fracture non-unions, and augmenting existing bone volumes. For the numerous patients taking… (more)

Subjects/Keywords: zoledronate; BMP; bone morphogenetic proteins; bisphosphonates; bone graft; 0567

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APA (6^th Edition):

Prichert, M. (2011). The Effect of Zoledronate Pretreatment on BMP Induced Bone Formation in Mice. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/31392

Chicago Manual of Style (16^th Edition):

Prichert, Marina. “The Effect of Zoledronate Pretreatment on BMP Induced Bone Formation in Mice.” 2011. Masters Thesis, University of Toronto. Accessed March 05, 2021. http://hdl.handle.net/1807/31392.

MLA Handbook (7^th Edition):

Prichert, Marina. “The Effect of Zoledronate Pretreatment on BMP Induced Bone Formation in Mice.” 2011. Web. 05 Mar 2021.

Vancouver:

Prichert M. The Effect of Zoledronate Pretreatment on BMP Induced Bone Formation in Mice. [Internet] [Masters thesis]. University of Toronto; 2011. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/1807/31392.

Council of Science Editors:

Prichert M. The Effect of Zoledronate Pretreatment on BMP Induced Bone Formation in Mice. [Masters Thesis]. University of Toronto; 2011. Available from: http://hdl.handle.net/1807/31392

Drexel University

6. Yu, Aaron. Design and Characterization of an Intraoperatively Loaded Protein Delivery Device for the Treatment of Open Tibial Fractures.

Degree: 2013, Drexel University

URL: http://hdl.handle.net/1860/idea:7115

► The FDA approved INFUSE Bone Graft is the standard of care for the therapeutic delivery of BMP-2 in open tibial fractures stabilized via intramedullary nailing.… (more)

▼ The FDA approved INFUSE Bone Graft is the standard of care for the therapeutic delivery of BMP-2 in open tibial fractures stabilized via intramedullary nailing. Though the device is considered clinically effective, several serious and potentially preventable side effects induced by the Bone Graft's initial burst release characteristics, such as massive swelling, cystic bone growth, and ectopic bone growth have negatively impacted the lives of countless patients. Based on analysis of recent studies involving BMP-2 delivery technologies, it was determined that a lower dose, sustained release of BMP-2, averaging 1.37 to 25.7 μg per day over at the duration of at least 2-4 weeks, absent a burst release, would dramatically decrease the chances of adverse side effects while still providing the therapeutic benefits of BMP-2. Several new technologies have been developed that provide similar release profiles to the abovementioned, and they have shown much promise in several in vivo and in vitro studies. However, the majority of those technologies rely on pre-encapsulation of the BMP-2 and harsh polymer processing techniques that facilitate denaturation of the protein. Intraoperative loading circumvents this shortcoming, since the BMP-2 is stored in its stable, lyophilized form until needed. Over the course of this design project, a novel, biodegradable protein delivery device was developed in an attempt to combine the advantages of intraoperative loading with a more clinically relevant delivery profile for BMP-2. The proposed design consisted of a two component system comprising a Polycaprolactone : PEG 400 composite outer pouch with an internal, fibrous sodium alginate sponge. The system was designed for intraoperative loading using a technique similar to the INFUSE Bone Graft, where a reconstituted model protein solution (bovine serum albumin) is injected into the PCL: PEG 400 pouch containing an alginate sponge. After a brief 5 minute wait, a 10 % w/v CaCl2 solution is injected into the pouch, causing crosslinking of the swelled alginate and encapsulation of the protein. The pouch is then tightly sealed with a solvent bonding approach using ethyl acetate. This pouch design allows for successful loading with reconstituted protein solution within 20 minutes. The release characteristics of the device, as well as alternative preparation methods and their effect on the pouch release rate were investigated through dissolution testing in simulated physiological conditions (PBS, pH 7.4 & 37ºC) and the Bradford-Coomassie assay. Several pouch formulations were assessed, and three were capable of providing sustained release of BSA in the desired 1.37-25.7 μg range over 14 days, with no burst release. It was determined that modifying the ratio of PCL to PEG 400 would cause a predictable change in the release profile of the pouches, in that the higher the PCL : PEG 400 ratio, the slower the release. In addition to the protein delivery aspects, the material degradation and surface morphologies of the pouch were monitored over the… Advisors/Committee Members: Wheatley, Margaret A., School of Biomedical Engineering, Science and Health Systems.

Subjects/Keywords: Biomedical engineering; Bone-grafting; Bone morphogenetic proteins – Therapeutic use

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APA (6^th Edition):

Yu, A. (2013). Design and Characterization of an Intraoperatively Loaded Protein Delivery Device for the Treatment of Open Tibial Fractures. (Thesis). Drexel University. Retrieved from http://hdl.handle.net/1860/idea:7115

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Yu, Aaron. “Design and Characterization of an Intraoperatively Loaded Protein Delivery Device for the Treatment of Open Tibial Fractures.” 2013. Thesis, Drexel University. Accessed March 05, 2021. http://hdl.handle.net/1860/idea:7115.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Yu, Aaron. “Design and Characterization of an Intraoperatively Loaded Protein Delivery Device for the Treatment of Open Tibial Fractures.” 2013. Web. 05 Mar 2021.

Vancouver:

Yu A. Design and Characterization of an Intraoperatively Loaded Protein Delivery Device for the Treatment of Open Tibial Fractures. [Internet] [Thesis]. Drexel University; 2013. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/1860/idea:7115.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Yu A. Design and Characterization of an Intraoperatively Loaded Protein Delivery Device for the Treatment of Open Tibial Fractures. [Thesis]. Drexel University; 2013. Available from: http://hdl.handle.net/1860/idea:7115

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Texas Southwestern Medical Center

7. Coster, Adam D. Quantitative Single-Cell Imaging Reveals Insulation of Morphogenic Signal Transduction.

Degree: 2014, University of Texas Southwestern Medical Center

URL: http://hdl.handle.net/2152.5/3586

► How cells integrate external cues in order to make behavioral decisions is a central problem of cell biology. In development and in tissue-homeostasis, cell-fate decisions… (more)

Subjects/Keywords: Bone Morphogenetic Proteins; Signal Transduction; Transforming Growth Factor beta; Wnt Proteins

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APA (6^th Edition):

Coster, A. D. (2014). Quantitative Single-Cell Imaging Reveals Insulation of Morphogenic Signal Transduction. (Thesis). University of Texas Southwestern Medical Center. Retrieved from http://hdl.handle.net/2152.5/3586

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Coster, Adam D. “Quantitative Single-Cell Imaging Reveals Insulation of Morphogenic Signal Transduction.” 2014. Thesis, University of Texas Southwestern Medical Center. Accessed March 05, 2021. http://hdl.handle.net/2152.5/3586.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Coster, Adam D. “Quantitative Single-Cell Imaging Reveals Insulation of Morphogenic Signal Transduction.” 2014. Web. 05 Mar 2021.

Vancouver:

Coster AD. Quantitative Single-Cell Imaging Reveals Insulation of Morphogenic Signal Transduction. [Internet] [Thesis]. University of Texas Southwestern Medical Center; 2014. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/2152.5/3586.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Coster AD. Quantitative Single-Cell Imaging Reveals Insulation of Morphogenic Signal Transduction. [Thesis]. University of Texas Southwestern Medical Center; 2014. Available from: http://hdl.handle.net/2152.5/3586

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

8. Boulet, Nathalie. Rôles des Bone Morphogenetic Proteins dans la conversion adipocytaire et le développement du tissu adipeux humain : Roles of bone morphogenetic proteins in adipose conversion and human adipose tissue developement.

Degree: Docteur es, Physiopathologie, 2015, Université Toulouse III – Paul Sabatier

URL: http://www.theses.fr/2015TOU30187

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Les adipocytes (cellules spécialisées dans le stockage des graisses) sont formés à partir de cellules immatures appelées cellules progénitrices lors du processus d'adipogenèse. Chez l'homme,… (more)

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Les adipocytes (cellules spécialisées dans le stockage des graisses) sont formés à partir de cellules immatures appelées cellules progénitrices lors du processus d'adipogenèse. Chez l'homme, les différentes étapes de ce processus sont mal connues ainsi que les signaux qui le régulent. La première partie de mon travail de thèse a eu pour but de caractériser la cellule intermédiaire entre la cellule progénitrice et l'adipocyte : le préadipocyte. La deuxième partie a consisté à évaluer le rôle des protéines morphogénétiques de l'os (ou BMP), des inducteurs de l'adipogenèse décrits chez la souris, dans l'adipogenèse humaine. Nous avons montré que les BMP2, 4 et 7 sont produites dans le tissu gras humain et BMP7 est modulée par l'obésité. Les BMP2 et 4 induisent l'adipogenèse des cellules progénitrices humaines mais seule la BMP7 permet la production d'adipocytes particuliers " beiges " décrits pour consommer les lipides et produire de la chaleur. Ces travaux affinent nos connaissances sur les mécanismes impliqués dans l'expansion du tissu gras et permettront d'élaborer des stratégies pour lutter contre le développement des pathologies liées à l'obésité.

Adipocytes (cells specialized in fat storage) arise from immature cells, called progenitor cells, during the process of adipogenesis. In human, the different stages of adipogenesis are not well defined as well as the signals involved in adipogenic modulation. The first part of my thesis work aimed to characterize the intermediate cell state between progenitor cell and mature adipocyte: the preadipocyte. The second part aimed to evaluate the role of bone morphogenetic proteins (BMPs) in human adipogenesis. In mice, BMP2 and BMP4 induce classical adipogenesis whereas BMP7 leads to the production of "brite" adipocytes with the capacity to use lipids to produce heat. We have shown that BMP2, 4 and 7 are produced in human fat depots and BMP7 is modulated by obesity. BMP2 and 4 induce classical adipogenesis and BMP7 only induces brite adipogenesis from human progenitor cells. These works improve our knowledge about the mechanisms involved in the expansion of fat depot and may allow the identification of new strategies to fight against the development of obesity-associated pathologies.

Advisors/Committee Members: Galitzky, Jean (thesis director), Bouloumié, Anne (thesis director).

Subjects/Keywords: Adipogenèse; Bone Morphogenetic Proteins; Cellules progénitrices; Obésité; Localisation du tissu adipeux; Adipogenesis; Bone morphogenetic proteins; Progenitor cells; Obesity; Adipose tissue location

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APA (6^th Edition):

Boulet, N. (2015). Rôles des Bone Morphogenetic Proteins dans la conversion adipocytaire et le développement du tissu adipeux humain : Roles of bone morphogenetic proteins in adipose conversion and human adipose tissue developement. (Doctoral Dissertation). Université Toulouse III – Paul Sabatier. Retrieved from http://www.theses.fr/2015TOU30187

Chicago Manual of Style (16^th Edition):

Boulet, Nathalie. “Rôles des Bone Morphogenetic Proteins dans la conversion adipocytaire et le développement du tissu adipeux humain : Roles of bone morphogenetic proteins in adipose conversion and human adipose tissue developement.” 2015. Doctoral Dissertation, Université Toulouse III – Paul Sabatier. Accessed March 05, 2021. http://www.theses.fr/2015TOU30187.

MLA Handbook (7^th Edition):

Boulet, Nathalie. “Rôles des Bone Morphogenetic Proteins dans la conversion adipocytaire et le développement du tissu adipeux humain : Roles of bone morphogenetic proteins in adipose conversion and human adipose tissue developement.” 2015. Web. 05 Mar 2021.

Vancouver:

Boulet N. Rôles des Bone Morphogenetic Proteins dans la conversion adipocytaire et le développement du tissu adipeux humain : Roles of bone morphogenetic proteins in adipose conversion and human adipose tissue developement. [Internet] [Doctoral dissertation]. Université Toulouse III – Paul Sabatier; 2015. [cited 2021 Mar 05]. Available from: http://www.theses.fr/2015TOU30187.

Council of Science Editors:

Boulet N. Rôles des Bone Morphogenetic Proteins dans la conversion adipocytaire et le développement du tissu adipeux humain : Roles of bone morphogenetic proteins in adipose conversion and human adipose tissue developement. [Doctoral Dissertation]. Université Toulouse III – Paul Sabatier; 2015. Available from: http://www.theses.fr/2015TOU30187

University of Lund

9. Belfrage, Ola. Allograft bone in hip revision: the effect of locally applied pharmacological treatment.

Degree: 2014, University of Lund

URL: https://lup.lub.lu.se/record/4390448 ; https://portal.research.lu.se/ws/files/4009461/4390449.docx

► The clinical success of primary hip replacement is paramount but the need for revisions will continue to increase due to the increasing number of operated… (more)

▼ The clinical success of primary hip replacement is paramount but the need for revisions will continue to increase due to the increasing number of operated individuals. In Sweden, the number of hip revisions in 2012 exceeded 2,300. During implant loosening, some of the bone in the femur is lost, which can make the revision more difficult. One way of handling bone loss has been the impaction technique. Allograft bone is morsellised and impacted into the defect before a prosthesis is inserted. Mechanically, the allograft bone immediately contributes to prosthetic stability. Biologically, the graft triggers an inflammatory response with ingrowth of fibrous tissue and blood vessels, accompanied by osteoclasts from the host. Parts of the graft bone are resorbed and degraded, and are eventually at least partially replaced with new living bone. In our first hypothesis, we suggested that resorption of the allograft that is too fast could reduce stability and result in implant loosening. We believed that this could be inhibited by local treatment of the graft with a bisphosphonate. Our second hypothesis was that by adding bone-inductive BMP-7 to the bisphosphonate, new bone formation would also be stimulated, leading to an increase in stability. In papers I and II, the effect of the bisphosphonate zoledronate and BMP-7 on allografts was investigated in a bone conduction chamber in rats. We found a strong synergism of the combined treatment compared to the saline control. Local treatment with the bisphosphonate was efficient but it also tended to inhibit bone formation. In paper III, the same drugs were evaluated in a more clinically relevant prosthetic model in rabbits. A knee prosthesis was inserted into the tibia, which had been filled with impacted, morsellised allograft soaked in BMP-7 and/or zoledronate. Micro-CT showed increased bone density after zoledronate treatment relative to the saline control, but by histology the bone surrounding the prosthesis had a more unstable structure when BMP-7 was used combined with zoledronate. In paper IV, 30 patients had their femoral hip implant revised with the impaction bone grafting technique, and were randomised to have the bone graft soaked in either clodronate—a bisphosphonate— or saline. DXA scans were performed postoperatively and at 3 and 12 months to evaluate the effect of the study drug. Radiostereometry (RSA) was performed postoperatively, after 6 weeks, and after 3 and 12 months, to measure implant micromotion. Bone density and implant motion were similar in both groups and no significant differences were found. In conclusion, we found that local administration of bisphosphonate is effective in inhibiting bone graft resorption in animal models and that the mode of application may matter. The addition of BMP-7 may lead to reduced stability and cannot be recommended. Finally, we were unable to show any effect of the bisphosphonate clodronate on bone density or implant motion in our clinical study on hip revision with impaction bone grafting.

Subjects/Keywords: Orthopedics; allograft bone; bone transplantation; bisphosphonate; bone morphogenetic proteins; hip revision; radiostereometric analysis

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APA (6^th Edition):

Belfrage, O. (2014). Allograft bone in hip revision: the effect of locally applied pharmacological treatment. (Doctoral Dissertation). University of Lund. Retrieved from https://lup.lub.lu.se/record/4390448 ; https://portal.research.lu.se/ws/files/4009461/4390449.docx

Chicago Manual of Style (16^th Edition):

Belfrage, Ola. “Allograft bone in hip revision: the effect of locally applied pharmacological treatment.” 2014. Doctoral Dissertation, University of Lund. Accessed March 05, 2021. https://lup.lub.lu.se/record/4390448 ; https://portal.research.lu.se/ws/files/4009461/4390449.docx.

MLA Handbook (7^th Edition):

Belfrage, Ola. “Allograft bone in hip revision: the effect of locally applied pharmacological treatment.” 2014. Web. 05 Mar 2021.

Vancouver:

Belfrage O. Allograft bone in hip revision: the effect of locally applied pharmacological treatment. [Internet] [Doctoral dissertation]. University of Lund; 2014. [cited 2021 Mar 05]. Available from: https://lup.lub.lu.se/record/4390448 ; https://portal.research.lu.se/ws/files/4009461/4390449.docx.

Council of Science Editors:

Belfrage O. Allograft bone in hip revision: the effect of locally applied pharmacological treatment. [Doctoral Dissertation]. University of Lund; 2014. Available from: https://lup.lub.lu.se/record/4390448 ; https://portal.research.lu.se/ws/files/4009461/4390449.docx

University of Hong Kong

10. 陳慧朗. The protective role of bone morphogenetic protein-7 against mesangial cell injury in IgA nephropathy.

Degree: 2008, University of Hong Kong

URL: http://hdl.handle.net/10722/52131

Subjects/Keywords: Bone morphogenetic proteins.; IgA glomerulonephritis.

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

陳慧朗.. (2008). The protective role of bone morphogenetic protein-7 against mesangial cell injury in IgA nephropathy. (Thesis). University of Hong Kong. Retrieved from http://hdl.handle.net/10722/52131

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

陳慧朗.. “The protective role of bone morphogenetic protein-7 against mesangial cell injury in IgA nephropathy.” 2008. Thesis, University of Hong Kong. Accessed March 05, 2021. http://hdl.handle.net/10722/52131.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

陳慧朗.. “The protective role of bone morphogenetic protein-7 against mesangial cell injury in IgA nephropathy.” 2008. Web. 05 Mar 2021.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Vancouver:

陳慧朗.. The protective role of bone morphogenetic protein-7 against mesangial cell injury in IgA nephropathy. [Internet] [Thesis]. University of Hong Kong; 2008. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/10722/52131.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

陳慧朗.. The protective role of bone morphogenetic protein-7 against mesangial cell injury in IgA nephropathy. [Thesis]. University of Hong Kong; 2008. Available from: http://hdl.handle.net/10722/52131

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

Vanderbilt University

11. Clendenning, Dawn Elizabeth. Determining the role of Growth Differentiation Factor-6 (Gdf6) in the development of the coronal suture.

Degree: PhD, Human Genetics, 2012, Vanderbilt University

URL: http://hdl.handle.net/1803/12174

► HUMAN GENETICS DETERMINING THE ROLE OF GROWTH DIFFERENTIATION FACTOR-6 (GDF6) IN THE DEVELOPMENT OF THE CORONAL SUTURE DAWN ELIZABETH CLENDENNING Dissertation under the direction of… (more)

Subjects/Keywords: Bone Morphogenetic Proteins; skeletal development; craniosynostosis; cranial sutures; Gdf6

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Clendenning, D. E. (2012). Determining the role of Growth Differentiation Factor-6 (Gdf6) in the development of the coronal suture. (Doctoral Dissertation). Vanderbilt University. Retrieved from http://hdl.handle.net/1803/12174

Chicago Manual of Style (16^th Edition):

Clendenning, Dawn Elizabeth. “Determining the role of Growth Differentiation Factor-6 (Gdf6) in the development of the coronal suture.” 2012. Doctoral Dissertation, Vanderbilt University. Accessed March 05, 2021. http://hdl.handle.net/1803/12174.

MLA Handbook (7^th Edition):

Clendenning, Dawn Elizabeth. “Determining the role of Growth Differentiation Factor-6 (Gdf6) in the development of the coronal suture.” 2012. Web. 05 Mar 2021.

Vancouver:

Clendenning DE. Determining the role of Growth Differentiation Factor-6 (Gdf6) in the development of the coronal suture. [Internet] [Doctoral dissertation]. Vanderbilt University; 2012. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/1803/12174.

Council of Science Editors:

Clendenning DE. Determining the role of Growth Differentiation Factor-6 (Gdf6) in the development of the coronal suture. [Doctoral Dissertation]. Vanderbilt University; 2012. Available from: http://hdl.handle.net/1803/12174

University of Adelaide

12. Hussein, Tamer. Role of oocyte-secreted factors in prevention of cumulus cell apoptosis and enhancement of oocyte developmental competence.

Degree: 2006, University of Adelaide

URL: http://hdl.handle.net/2440/58191

► Paracrine factors secreted by the oocyte (oocyte-secreted factors, OSFs) regulate a broad range of cumulus cell functions. The capacity of oocytes to regulate their own… (more)

▼ Paracrine factors secreted by the oocyte (oocyte-secreted factors, OSFs) regulate a broad range of cumulus cell functions. The capacity of oocytes to regulate their own microenvironment by OSFs may in turn contribute to oocyte developmental competence. The aim of this thesis was to examine whether cumulus cells exhibit a low incidence of apoptosis due to their close association with oocytes and their exposure to OSFs, and to investigate if OSFs have a direct influence on bovine oocyte developmental competence during in vitro maturation (IVM). This thesis includes a series of studies designed to examine by various means the nature of the paracrine network of bone morphogenetic proteins (BMPs) and their binding proteins involved in the regulation of cumulus cell apoptosis. OSFs, in particular BMP- 15 and BMP-6, but not growth differentiation factor 9 (GDF-9), reduced apoptosis of cumulus cells by following a gradient from the site of the oocytes. Morever, follistatin and a BMP6 neutralizing antibody, which antagonized the anti-apoptotic effects of BMP15 and BMP6, respectively, whether alone or combined, blocked ~50% of the antiapoptotic actions of oocytes. These results demonstrated that OSFs, particularly BMP-15 and BMP-6, maintain the low incidence of apoptosis by establishing a localized gradient of bone morphogenetic proteins. Results from the present thesis also demonstrated that OSFs enhance oocyte developmental competence during IVM, whether in their native form as an uncharacterized mix of growth factors secreted by the oocyte, throughout the oocyte maturation period, or as exogenous BMP-15 and GDF-9, during the first 9 hour of IVM. Also, OSFs improved embryo quality as evident by increased blastocyst total and trophectoderm cell numbers. These results were further verified in neutralization experiments of the exogenous growth factors and of the native OSFs. Follistatin and the kinase inhibitor SB-431542, which antagonize BMP-15 and GDF-9, respectively, neutralized the stimulatory effects of the exogenous growth factors, and impaired the developmental competence of control cumulus-oocyte complexes (COCs). The work presented in this thesis has provided multiple lines of evidence that OSFregulation of the COC microenvironment is an important determinant of cumulus cell apoptosis and oocyte developmental programming. Advisors/Committee Members: School of Paediatrics and Reproductive Health : Obstetrics and Gynaecology (school).

Subjects/Keywords: oocyte; bone morphogenetic proteins

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hussein, T. (2006). Role of oocyte-secreted factors in prevention of cumulus cell apoptosis and enhancement of oocyte developmental competence. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/58191

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Hussein, Tamer. “Role of oocyte-secreted factors in prevention of cumulus cell apoptosis and enhancement of oocyte developmental competence.” 2006. Thesis, University of Adelaide. Accessed March 05, 2021. http://hdl.handle.net/2440/58191.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Hussein, Tamer. “Role of oocyte-secreted factors in prevention of cumulus cell apoptosis and enhancement of oocyte developmental competence.” 2006. Web. 05 Mar 2021.

Vancouver:

Hussein T. Role of oocyte-secreted factors in prevention of cumulus cell apoptosis and enhancement of oocyte developmental competence. [Internet] [Thesis]. University of Adelaide; 2006. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/2440/58191.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hussein T. Role of oocyte-secreted factors in prevention of cumulus cell apoptosis and enhancement of oocyte developmental competence. [Thesis]. University of Adelaide; 2006. Available from: http://hdl.handle.net/2440/58191

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Hong Kong University of Science and Technology

13. Ang, Ying Jin. Characterization of a BMP negative regulator, LON-2, in the extracellular matrix.

Degree: 2013, Hong Kong University of Science and Technology

URL: http://repository.ust.hk/ir/Record/1783.1-62357 ; https://doi.org/10.14711/thesis-b1251007 ; http://repository.ust.hk/ir/bitstream/1783.1-62357/1/th_redirect.html

► The conserved Bone Morphogenetic Protein (BMP) pathway impacts on a myriad of developmental processes in animals through the receptor relay molecules that target nuclear genes… (more)

Subjects/Keywords: Bone morphogenetic proteins ; Growth ; Regulation ; Caenorhabditis elegans ; Genetics

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ang, Y. J. (2013). Characterization of a BMP negative regulator, LON-2, in the extracellular matrix. (Thesis). Hong Kong University of Science and Technology. Retrieved from http://repository.ust.hk/ir/Record/1783.1-62357 ; https://doi.org/10.14711/thesis-b1251007 ; http://repository.ust.hk/ir/bitstream/1783.1-62357/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ang, Ying Jin. “Characterization of a BMP negative regulator, LON-2, in the extracellular matrix.” 2013. Thesis, Hong Kong University of Science and Technology. Accessed March 05, 2021. http://repository.ust.hk/ir/Record/1783.1-62357 ; https://doi.org/10.14711/thesis-b1251007 ; http://repository.ust.hk/ir/bitstream/1783.1-62357/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ang, Ying Jin. “Characterization of a BMP negative regulator, LON-2, in the extracellular matrix.” 2013. Web. 05 Mar 2021.

Vancouver:

Ang YJ. Characterization of a BMP negative regulator, LON-2, in the extracellular matrix. [Internet] [Thesis]. Hong Kong University of Science and Technology; 2013. [cited 2021 Mar 05]. Available from: http://repository.ust.hk/ir/Record/1783.1-62357 ; https://doi.org/10.14711/thesis-b1251007 ; http://repository.ust.hk/ir/bitstream/1783.1-62357/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ang YJ. Characterization of a BMP negative regulator, LON-2, in the extracellular matrix. [Thesis]. Hong Kong University of Science and Technology; 2013. Available from: http://repository.ust.hk/ir/Record/1783.1-62357 ; https://doi.org/10.14711/thesis-b1251007 ; http://repository.ust.hk/ir/bitstream/1783.1-62357/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Hong Kong University of Science and Technology

14. Luk, Chak Hon. Characterization of the regulatory role of Sma/Mab pathway by SMA-10/LRIG in caenorhabditis elegans.

Degree: 2016, Hong Kong University of Science and Technology

URL: http://repository.ust.hk/ir/Record/1783.1-87401 ; https://doi.org/10.14711/thesis-b1626282 ; http://repository.ust.hk/ir/bitstream/1783.1-87401/1/th_redirect.html

► The Bone Morphogenetic Protein (BMP) Signaling Pathway is evolutionarily conserved with significant roles in regulations of multiple developmental processes. Its regulation is achieved by modulation… (more)

Subjects/Keywords: Growth factors ; Bone morphogenetic proteins ; Caenorhabditis elegans ; Growth

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Luk, C. H. (2016). Characterization of the regulatory role of Sma/Mab pathway by SMA-10/LRIG in caenorhabditis elegans. (Thesis). Hong Kong University of Science and Technology. Retrieved from http://repository.ust.hk/ir/Record/1783.1-87401 ; https://doi.org/10.14711/thesis-b1626282 ; http://repository.ust.hk/ir/bitstream/1783.1-87401/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Luk, Chak Hon. “Characterization of the regulatory role of Sma/Mab pathway by SMA-10/LRIG in caenorhabditis elegans.” 2016. Thesis, Hong Kong University of Science and Technology. Accessed March 05, 2021. http://repository.ust.hk/ir/Record/1783.1-87401 ; https://doi.org/10.14711/thesis-b1626282 ; http://repository.ust.hk/ir/bitstream/1783.1-87401/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Luk, Chak Hon. “Characterization of the regulatory role of Sma/Mab pathway by SMA-10/LRIG in caenorhabditis elegans.” 2016. Web. 05 Mar 2021.

Vancouver:

Luk CH. Characterization of the regulatory role of Sma/Mab pathway by SMA-10/LRIG in caenorhabditis elegans. [Internet] [Thesis]. Hong Kong University of Science and Technology; 2016. [cited 2021 Mar 05]. Available from: http://repository.ust.hk/ir/Record/1783.1-87401 ; https://doi.org/10.14711/thesis-b1626282 ; http://repository.ust.hk/ir/bitstream/1783.1-87401/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Luk CH. Characterization of the regulatory role of Sma/Mab pathway by SMA-10/LRIG in caenorhabditis elegans. [Thesis]. Hong Kong University of Science and Technology; 2016. Available from: http://repository.ust.hk/ir/Record/1783.1-87401 ; https://doi.org/10.14711/thesis-b1626282 ; http://repository.ust.hk/ir/bitstream/1783.1-87401/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Southern California

15. Hazen, Virginia M. The diverse roles of smads in dorsal spinal cord development.

Degree: PhD, Neuroscience, 2011, University of Southern California

URL: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll127/id/656050/rec/6578

► Bone Morphogenetic Proteins (BMPs) have disparate functions establishing neural circuitry in the dorsal spinal cord. BMPs first induce specific neuronal cell fates, including the most… (more)

▼ Bone Morphogenetic Proteins (BMPs) have disparate functions establishing neural circuitry in the dorsal spinal cord. BMPs first induce specific neuronal cell fates, including the most dorsal commissural sensory neurons, dorsal interneuron (dI) 1 population. Subsequently, the BMPs act as a guidance cue to direct dI1 axons away from the dorsal midline. Here, we assess how dI1 neurons interpret the BMPs to accomplish these diverse cellular responses. In the traditional signaling cascade, BMPs specify cell fate by binding to a complex of BMP receptors (Bmprs) and thereby activating the Smad family of transcriptional regulators. Our previous studies have shown that the Bmprs also mediate the guidance activities of the BMPs. However, it remains unresolved which intracellular effectors are activated by the Bmprs to control axon dynamics. To further understand the role of the canonical BMP signaling pathway in establishing neural circuitry, we have examined whether the Smad complex, which acts downstream of the Bmprs, also regulates cell fate determination and axonal pathfinding. ❧ The evolutionarily conserved Smad complex is comprised of a combination of the BMP specific receptor-activated (R) Smads, Smad1, Smad5 and Smad8, and common mediator (Co) Smad4. Inhibitory (I) Smads, Smad6 and Smad7, block the signaling of this complex. To elucidate whether these Smads mediate the disparate tasks of BMP signaling in dorsal spinal cord development, we employed various gain and loss-of-function techniques to test the sufficiency and requirement of the Smads in dorsal cell fate specification and axon dynamics. ❧ We first looked at how functionally blocking BMP signaling via the overexpression of I-Smads, Smad6 and Smad7, would affect these processes. Unexpectedly, our experiments in chicken embryos revealed that Smad6 and Smad7 have dissimilar expression patterns in the developing spinal cord and in accordance with their expression distribution, regulate the distinct outcomes of BMP signaling. In particular, Smad7 primarily limits the role of BMPs to specify the dorsal-most neurons, whereas only Smad6 acts to inhibit the outgrowth of dI1 axons. ❧ Next, we investigated the role of the BMP specific R-Smads, Smad1, Smad5 and Smad8, in orchestrating cell fate determination and axon guidance events. Similar to the I-Smads, these R-Smads have strikingly divergent expression patterns in the developing spinal cord suggesting that these Smads also differentially affect BMP mediated cell fate decisions and axonal outgrowth. Supporting this hypothesis, when Smad5, but not Smad1, function is decreased in chicken and mouse embryos, there is a loss of dorsal neural progenitors and neurons. In contrast, only Smad1 is necessary to control dI1 axon outgrowth in our loss-of-function studies. ❧ Taken together, these results suggest that different components of the canonical Smad signaling pathway are regulating the disparate outcomes of BMP signaling. Specifically, Smad5 and Smad7 are primarily involved in dorsal cell specification, whereas Smad1 and… Advisors/Committee Members: Butler, Samantha J. (Committee Chair), Bottjer, Sarah W. (Committee Member), Maxson, Robert E. (Committee Member), Watts, Alan G. (Committee Member).

Subjects/Keywords: axon guidance; cell fate; Smads; spinal cord; Bone Morphogenetic Proteins ❧

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hazen, V. M. (2011). The diverse roles of smads in dorsal spinal cord development. (Doctoral Dissertation). University of Southern California. Retrieved from http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll127/id/656050/rec/6578

Chicago Manual of Style (16^th Edition):

Hazen, Virginia M. “The diverse roles of smads in dorsal spinal cord development.” 2011. Doctoral Dissertation, University of Southern California. Accessed March 05, 2021. http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll127/id/656050/rec/6578.

MLA Handbook (7^th Edition):

Hazen, Virginia M. “The diverse roles of smads in dorsal spinal cord development.” 2011. Web. 05 Mar 2021.

Vancouver:

Hazen VM. The diverse roles of smads in dorsal spinal cord development. [Internet] [Doctoral dissertation]. University of Southern California; 2011. [cited 2021 Mar 05]. Available from: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll127/id/656050/rec/6578.

Council of Science Editors:

Hazen VM. The diverse roles of smads in dorsal spinal cord development. [Doctoral Dissertation]. University of Southern California; 2011. Available from: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll127/id/656050/rec/6578

Rutgers University

16. Niepielko, Matthew Gene, 1985-. Changes in BMP and EGFR signaling components underlie the evolution of Drosophila eggshell morphologies.

Degree: Computational and Integrative Biology, 2014, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/43548/

Subjects/Keywords: Drosophila – Morphogenesis; Bone morphogenetic proteins

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Niepielko, Matthew Gene, 1. (2014). Changes in BMP and EGFR signaling components underlie the evolution of Drosophila eggshell morphologies. (Thesis). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/43548/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Niepielko, Matthew Gene, 1985-. “Changes in BMP and EGFR signaling components underlie the evolution of Drosophila eggshell morphologies.” 2014. Thesis, Rutgers University. Accessed March 05, 2021. https://rucore.libraries.rutgers.edu/rutgers-lib/43548/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Niepielko, Matthew Gene, 1985-. “Changes in BMP and EGFR signaling components underlie the evolution of Drosophila eggshell morphologies.” 2014. Web. 05 Mar 2021.

Vancouver:

Niepielko, Matthew Gene 1. Changes in BMP and EGFR signaling components underlie the evolution of Drosophila eggshell morphologies. [Internet] [Thesis]. Rutgers University; 2014. [cited 2021 Mar 05]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/43548/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Niepielko, Matthew Gene 1. Changes in BMP and EGFR signaling components underlie the evolution of Drosophila eggshell morphologies. [Thesis]. Rutgers University; 2014. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/43548/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Texas Southwestern Medical Center

17. Danesh, Shahab Malekpour. BMP Signaling through BMPR1A is Required for Establishment of Pancreatic Laterality.

Degree: 2009, University of Texas Southwestern Medical Center

URL: http://hdl.handle.net/2152.5/354

► Pancreatic development begins in the mouse, at embryonic day 8.5, as two patches within the gut tube that are located between the stomach and the… (more)

▼ Pancreatic development begins in the mouse, at embryonic day 8.5, as two patches within the gut tube that are located between the stomach and the duodenum. These patches begin to bud, proliferate, and differentiate to become the dorsal and ventral pancreas. During pancreatic bud development several tissues that are adjacent to the gut tube provide important signals for pancreatic development. Various bone morphogenetic proteins (Bmps) and Bmp receptors (Bmprs) are expressed in the pancreas and in the tissues that flank the pancreatic bud during pancreatic development. Bmp7, BmpR1a, and BmpRII are expressed in the pancreatic endoderm during pancreatic bud development. Bmp2, Bmp4, BmpR1a and BmpRII are expressed in the dorsal aorta which transiently flanks the pancreatic bud. Bmp4, Bmp7, BmpR1a, BmpR1b, and BmpRII are expressed in the pre-pancreatic mesenchyme. Interestingly, Bmp4 is expressed asymmetrically along the gut in the mesogastrium, preceeding gut turning. Transgenic knock down of BMP signaling via the secreted BMP antagonist Noggin in the pre-pancreatic milieu results in reduced pancreas, spleen, stomach and in failure of pyloric sphincter formation. Interestingly, BMP knockdown also results in defects in lateral growth of the pancreas. Specifically, BMP knockdown prevents formation of the splanchnic mesodermal plate (SMP), the asymmetrically forming mesothelial structure that accompanies leftward growth of the pancreas. Conversely, over expression of Bmp2 in the pre-pancreatic milieu does not result in defects in SMP formation or lateral growth of the pancreas. However, overexpression of Bmp2 in the pancreas epithelium resulted in failure to differentiate into endocrine and exocrine cell lineages. Global knockout of BmpR1a, but not BmprII, prior to pancreatic bud development results in developmental defects in SMP formation and pancreatic laterality. Bmpr1a knockout results in reduced SMP expression of Bapx1, a gene required for SMP formation and lateral growth of the pancreas. Therefore, defects in lateral growth of the pancreas in Bmpr1a mutants are likely mediated by Bapx1 and, accordingly, Bmpr1a mutant pancreata exhibit misregulation of genes that require Bapx1, including Fgf9 and Fgf10. Additionally, deletion of Bmpr1a also leads to misregulation of Barx1, another gene with SMP restricted expression. Deletion of BmpR1a specifically from the pre-pancreatic endoderm or endothelial (aorta) cells did not show defects with respect to pancreatic growth, development, or differentiation. Finally, work herein shows that BMP-BMPR1A signaling to the endothelium specifically is required for vascularization of the pancreatic bud and SMP formation. This study presents the first evidence of a cell-cell signaling molecule playing a role in left-right patterning during organogenesis. Advisors/Committee Members: Cleaver, Ondine.

Subjects/Keywords: Bone Morphogenetic Protein Receptors; Bone Morphogenetic Proteins; Pancreas

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Danesh, S. M. (2009). BMP Signaling through BMPR1A is Required for Establishment of Pancreatic Laterality. (Thesis). University of Texas Southwestern Medical Center. Retrieved from http://hdl.handle.net/2152.5/354

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Danesh, Shahab Malekpour. “BMP Signaling through BMPR1A is Required for Establishment of Pancreatic Laterality.” 2009. Thesis, University of Texas Southwestern Medical Center. Accessed March 05, 2021. http://hdl.handle.net/2152.5/354.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Danesh, Shahab Malekpour. “BMP Signaling through BMPR1A is Required for Establishment of Pancreatic Laterality.” 2009. Web. 05 Mar 2021.

Vancouver:

Danesh SM. BMP Signaling through BMPR1A is Required for Establishment of Pancreatic Laterality. [Internet] [Thesis]. University of Texas Southwestern Medical Center; 2009. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/2152.5/354.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Danesh SM. BMP Signaling through BMPR1A is Required for Establishment of Pancreatic Laterality. [Thesis]. University of Texas Southwestern Medical Center; 2009. Available from: http://hdl.handle.net/2152.5/354

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Université Catholique de Louvain

18. Kuterbekov, Mirasbek. Polymeric microcarriers with bioactive coatings for the osteogenic differentiation of human adipose stromal cells.

Degree: 2019, Université Catholique de Louvain

URL: http://hdl.handle.net/2078.1/216773

►

The regeneration of critical-sized bone defects remains a major healthcare challenge. The limits of tissue grafting prompted us to develop a synthetic alternative based on… (more)

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The regeneration of critical-sized bone defects remains a major healthcare challenge. The limits of tissue grafting prompted us to develop a synthetic alternative based on biomaterial constructs, osteoinductive factors and stromal/stem cells. For biomaterial constructs, we focused on porous polymeric microcarriers as they support large-scale cell expansion and modular tissue assembly, circumventing two crucial bottlenecks for clinical translation. To facilitate industrial supply and regulatory approval, we designed a solvent-free method for their fabrication based on the spherulitic crystallization of poly(L-lactide) (PLLA) in its blends with polyethylene glycol (PEG). The PLLA spherulites were easily recovered as microcarriers by rinsing away the water-soluble PEG. Their size and porosity could be independently controlled by tuning the PLLA/PEG ratio and crystallization temperature. The biocompatibility and osteoconductivity of PLLA microcarriers were confirmed through the expansion and osteogenic differentiation of human adipose stromal cells (hASCs). Because the latter hASC function is sensitive to different culture parameters, we then used the Design of Experiments for their rapid screening. In combination with high-throughput analysis, we identified a strong effect of serum, hASC process, ascorbate and bone morphogenetic protein 9 (BMP-9) on the osteogenic differentiation of hASCs. Finally, to deliver osteoinductive factors, we elaborated polyelectrolyte multilayers (PEMs) based on biocompatible poly(L-ornithine) and hyaluronic acid. These PEMs were characterized in terms of their growth, morphology, the ability to incorporate different BMPs and to function as coatings on PLLA microcarriers. Our preliminary results showed that the incorporation of BMPs inside PEMs had a strong effect on hASC adhesion. While further studies are needed, PLLA microcarriers coated with BMP-loaded PEMs and seeded with hASCs could be a promising synthetic implant for bone regeneration.

(FSA - Sciences de l'ingénieur) – UCL, 2019

Advisors/Committee Members: UCL - SST/IMCN/BSMA - Bio and soft matter, UCL - Ecole Polytechnique de Louvain, Jonas, Alain, Glinel, Karine, Picart, Catherine, Lavalle, Philippe, Miettinen, Susanna, Noël, Daniele.

Subjects/Keywords: Bone morphogenetic proteins; Microcarriers; Human adipose stromal cells; Polyelectrolyte multilayers; Osteogenic differentiation; Bone tissue engineering

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kuterbekov, M. (2019). Polymeric microcarriers with bioactive coatings for the osteogenic differentiation of human adipose stromal cells. (Thesis). Université Catholique de Louvain. Retrieved from http://hdl.handle.net/2078.1/216773

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kuterbekov, Mirasbek. “Polymeric microcarriers with bioactive coatings for the osteogenic differentiation of human adipose stromal cells.” 2019. Thesis, Université Catholique de Louvain. Accessed March 05, 2021. http://hdl.handle.net/2078.1/216773.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kuterbekov, Mirasbek. “Polymeric microcarriers with bioactive coatings for the osteogenic differentiation of human adipose stromal cells.” 2019. Web. 05 Mar 2021.

Vancouver:

Kuterbekov M. Polymeric microcarriers with bioactive coatings for the osteogenic differentiation of human adipose stromal cells. [Internet] [Thesis]. Université Catholique de Louvain; 2019. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/2078.1/216773.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kuterbekov M. Polymeric microcarriers with bioactive coatings for the osteogenic differentiation of human adipose stromal cells. [Thesis]. Université Catholique de Louvain; 2019. Available from: http://hdl.handle.net/2078.1/216773

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Minnesota

19. Espe, Kelly Christine. Bone Morphogenetic Protein-2 (BMP2) upregulates osteoclast gene expression.

Degree: MS, Dentistry, 2009, University of Minnesota

URL: http://purl.umn.edu/59781

► Bone Morphogenetic Proteins (BMPs) induce bone formation by osteoblasts, but their direct role in bone resorption by osteoclasts remains to be characterized. Twisted Gastrulation (Twsg1)… (more)

Subjects/Keywords: Bone Morphogenetic Proteins; Bone; Osteoclasts; Dentistry

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Espe, K. C. (2009). Bone Morphogenetic Protein-2 (BMP2) upregulates osteoclast gene expression. (Masters Thesis). University of Minnesota. Retrieved from http://purl.umn.edu/59781

Chicago Manual of Style (16^th Edition):

Espe, Kelly Christine. “Bone Morphogenetic Protein-2 (BMP2) upregulates osteoclast gene expression.” 2009. Masters Thesis, University of Minnesota. Accessed March 05, 2021. http://purl.umn.edu/59781.

MLA Handbook (7^th Edition):

Espe, Kelly Christine. “Bone Morphogenetic Protein-2 (BMP2) upregulates osteoclast gene expression.” 2009. Web. 05 Mar 2021.

Vancouver:

Espe KC. Bone Morphogenetic Protein-2 (BMP2) upregulates osteoclast gene expression. [Internet] [Masters thesis]. University of Minnesota; 2009. [cited 2021 Mar 05]. Available from: http://purl.umn.edu/59781.

Council of Science Editors:

Espe KC. Bone Morphogenetic Protein-2 (BMP2) upregulates osteoclast gene expression. [Masters Thesis]. University of Minnesota; 2009. Available from: http://purl.umn.edu/59781

20. Cestari, Tania Mary. Aspectos celulares, teciduais e moleculares da osteogênese ectópica e ortotópica induzida pela matriz alogênica óssea e dentinária.

Degree: PhD, Biologia Oral, 2009, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/25/25142/tde-02062009-111408/ ;

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O objetivo do atual trabalho, foi correlacionar os eventos celulares e teciduais com a expressão das proteínas VEGF, BMP-7, RANKL e OPG durante a osteogênese… (more)

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O objetivo do atual trabalho, foi correlacionar os eventos celulares e teciduais com a expressão das proteínas VEGF, BMP-7, RANKL e OPG durante a osteogênese ectópica e ortotópica, induzida pela matriz óssea (MO) e dentinária (MD) alogênica. Matrizes alogênicas desmineralizada em HCl a 0,6N, obtidas de fêmur e incisivo de ratos, fori implantada entre as fáscias musculares da coxa e em defeito trans-ósseo de 8mm de diâmetro nos ossos parietais. As análises radiográfica e histomorfométrica da neoformação óssea e, a imunohistoquímica e o western blotting para as proteínas VEGF, BMP, RANKL e OPG, mostraram que: a) o volume da região do enxerto nos sítios ortotópicos reduziu 19% em 42 dias; b) em ambos tipos de enxerto e locais de implantação, ocorreu formação de tecido cartilaginoso e ósseo; c) nos sítios intramusculares, a reabsorção da matriz alogênica e a remodelação do tecido cartilaginoso, ósseo e medular foi mais acelerado, em relação a implantação ortotópico; d) o aumento na densidade de volume dos vasos sanguíneos e no número de osteoblastos/osteócitos e osteoclastos ocorreu simultaneamente e estava associado à maior reabsorção da matriz alogênica e à formação do tecido medular (hematopoiético); e) as proteínas VEGF, BMP-7, RANKL, OPG foram expressas em condrócitos, osteoblastos ativos, osteócitos recém aprisionados na matriz e em células estromais próximas aos osteoblastos ou às áreas da matriz alogênica reabsorvida; e f) a expressão das proteínas VEGF, BMP-7, RANKL e OPG foi maior no grupo MO. O pico de expressão dessas proteínas ocorreu nos períodos de 14 aos 21 dias no grupo da MO e 21 e 28 dias no grupo da MD. Concluímos que, a capacidade osteoindutora da matriz alogênica desmineralizada está relacionado a origem da matriz e ao sítio de implantação e que, as proteínas VEGF, BMP-7, RANKL e OPG estão associadas a maior reabsorção da matriz implantada, promovendo uma rápida e contínua liberação dos morfógenos contidos em seu interior que, induzem temporal e espacialmente a formação óssea/medular.

The aim of the present work was to correlate the cellular and tissue events with the expression of VEGF, BMP-7, RANKL and OPG during ectopic and orthotopic osteogenesis, induced by bone and dentin allogeneic matrix. Allogenic matrices obtained from femur and incisor of rats and demineralized in 0.6 N HCl were implanted into a intramuscular pocket and a 8mm-diameter bone defect in the skull. The radiographic and histomorphometric analysis of new bone formation, and immunohistochemistry and western blotting for VEGF, BMP, RANKL and OPG proteins, showed that: a) the total volume of the graft region in orthotopic site decreased 19% at 42 days b) in both graft types and implantation sites occurred formation of cartilaginous and bone tissues, c) in intramuscular sites, the resorption of allogenic matrix and remodeling of the new formed cartilage and bone were faster, in relation to orthotopic implantation sites; d) the increase in the volume density of blood vessels and in the number of osteoblasts/osteocytes and…

Advisors/Committee Members: Taga, Rumio.

Subjects/Keywords: Angiogenic proteins; Bone matrix; Bone morphogenetic proteins; Bone reabsorption; Matriz óssea; Osteogenese; Osteogenesis; Proteína morfogenética óssea; Proteínas angiogenicas; Reabsorção óssea

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Cestari, T. M. (2009). Aspectos celulares, teciduais e moleculares da osteogênese ectópica e ortotópica induzida pela matriz alogênica óssea e dentinária. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/25/25142/tde-02062009-111408/ ;

Chicago Manual of Style (16^th Edition):

Cestari, Tania Mary. “Aspectos celulares, teciduais e moleculares da osteogênese ectópica e ortotópica induzida pela matriz alogênica óssea e dentinária.” 2009. Doctoral Dissertation, University of São Paulo. Accessed March 05, 2021. http://www.teses.usp.br/teses/disponiveis/25/25142/tde-02062009-111408/ ;.

MLA Handbook (7^th Edition):

Cestari, Tania Mary. “Aspectos celulares, teciduais e moleculares da osteogênese ectópica e ortotópica induzida pela matriz alogênica óssea e dentinária.” 2009. Web. 05 Mar 2021.

Vancouver:

Cestari TM. Aspectos celulares, teciduais e moleculares da osteogênese ectópica e ortotópica induzida pela matriz alogênica óssea e dentinária. [Internet] [Doctoral dissertation]. University of São Paulo; 2009. [cited 2021 Mar 05]. Available from: http://www.teses.usp.br/teses/disponiveis/25/25142/tde-02062009-111408/ ;.

Council of Science Editors:

Cestari TM. Aspectos celulares, teciduais e moleculares da osteogênese ectópica e ortotópica induzida pela matriz alogênica óssea e dentinária. [Doctoral Dissertation]. University of São Paulo; 2009. Available from: http://www.teses.usp.br/teses/disponiveis/25/25142/tde-02062009-111408/ ;

Loma Linda University

21. Yalung, Jelson. Mechanical Evaluation of Mandibular Defects Restored with rhBMP-2: A Finite Element Model.

Degree: MS, Orthodontics and Dentofacial Orthopedics, 2012, Loma Linda University

URL: https://scholarsrepository.llu.edu/etd/107

► Introduction: The utilization of recombinant human Bone Morphogenetic Protein-2 (rh- BMP2) to form new bone has been shown to be a promising alternative to autogenous… (more)

▼ Introduction: The utilization of recombinant human Bone Morphogenetic Protein-2 (rh- BMP2) to form new bone has been shown to be a promising alternative to autogenous bone grafts. Understanding the biomechanical properties of rhBMP-2 restored mandibular defects would provide useful knowledge in the future success of orthopedic and dental treatment in patients who have had restoration of mandibular defects with rhBMP-2. The aim of the study was to evaluate and compare the biomechanical characteristics of rhBMP-2 regenerated bone in mandibular defects using 2 different concentrations of rhBMP-2 with a given carrier in non-human primates Material and Methods: Critical-sized defects (approximately 2.5 cm) were created in the mandibles of 6 adult male non-human primates. Each side of the mandibles received one of 2 carrier types: 1) 1.35 mg/mL rhBMP-2 combined with a collagen ceramic sponge (CCS) and 2) 0.75 mg/mL rhBMP-2 combined with CCS. All defects were stabilized with a titanium reconstruction plate. Young's modulus of 10 bone samples was calculated using the results from a tensile test of the samples. Density of the 10 samples was determined with a pycnometer. A 2-dimensional model of the mandible was virtually created to simulate the mandible for a given BMP/carrier group. Subdomains of the model included cortical bone, regenerated bone, periodontal ligament, enamel, and cementum. Boundary conditions of the subdomains were assigned using the biomechanical properties determined in the literature and the regenerated bone values were assigned based on prior testing. Finite Element Analysis was performed with the COMSOL Finite Element Modeling software to measure peak Von Mises stresses and surface displacement of the newly regenerated bone in response to a 150 N force of the masseter muscle. Results: There was no statistical difference between the mechanical properties among treatment groups. However, both treatment groups showed a statistically significant difference in stress distribution, displacement in the x-axis, and displacement in the yaxis when compared to the control group (p < 0.05) but no statistically significant difference when compared to each other (p < 0.05). Conclusions: The differences in stress distribution and displacement when comparing the treatment group to the control group indicate that the treatment group regenerated bone was less stiff which lead to more displacement in the mandible and higher stress in response to function. This may be attributed to the 6-month follow-up period where the regenerated bone did not complete mineralization. The similarity in mechanical properties, stress, and displacement between treatment groups indicate that a rhBMP-2 concentration of 0.75 mg/mL produced bone that was biomechanically comparable to a concentration of 1.35 mg/mL. Advisors/Committee Members: Caruso, Joseph M., Herford, Alan S., Rungcharassaeng, Kitichai.

Subjects/Keywords: Orthodontics and Orthodontology; Other Dentistry; Bone Morphogenetic Proteins; Bone Development; Growth Substances; Recombinant Proteins; Bone Morphogenetic Protein 2; Bone regeneration; Mandible - surgery; Mandibular defects; Recombinant human bone morphogenetic protein-2; bone growth

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Yalung, J. (2012). Mechanical Evaluation of Mandibular Defects Restored with rhBMP-2: A Finite Element Model. (Thesis). Loma Linda University. Retrieved from https://scholarsrepository.llu.edu/etd/107

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Yalung, Jelson. “Mechanical Evaluation of Mandibular Defects Restored with rhBMP-2: A Finite Element Model.” 2012. Thesis, Loma Linda University. Accessed March 05, 2021. https://scholarsrepository.llu.edu/etd/107.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Yalung, Jelson. “Mechanical Evaluation of Mandibular Defects Restored with rhBMP-2: A Finite Element Model.” 2012. Web. 05 Mar 2021.

Vancouver:

Yalung J. Mechanical Evaluation of Mandibular Defects Restored with rhBMP-2: A Finite Element Model. [Internet] [Thesis]. Loma Linda University; 2012. [cited 2021 Mar 05]. Available from: https://scholarsrepository.llu.edu/etd/107.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Yalung J. Mechanical Evaluation of Mandibular Defects Restored with rhBMP-2: A Finite Element Model. [Thesis]. Loma Linda University; 2012. Available from: https://scholarsrepository.llu.edu/etd/107

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

22. V. Ceresoli. PRODUCTION OF HUMAN RECOMBINANT DIFFERENTIATION FACTORS IN TRANSGENIC TOBACCO PLANTS.

Degree: 2015, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/263847

► Research on bone regeneration began decades ago as a result of intensive studies on bone growth and healing. Bone has been recognised, among the many… (more)

▼ Research on bone regeneration began decades ago as a result of intensive studies on bone growth and healing. Bone has been recognised, among the many tissues in human body, as having the highest potential for regeneration, and it is the second most transplanted tissue following blood. Due to both internal mediators and external mechanical demands, it possesses the intrinsic ability for regeneration and is constantly engaged in a cycle of resorption and renewal undergoing continual chemical exchange and structural remodelling throughout adult life as well as during repair process in response to injury. Despite these abilities, beyond a critical point clinical intervention measures are needed; there are different clinical conditions requiring a large quantity of bone regeneration, such as for skeletal reconstruction of large bone defects created by trauma, infection, or cases in which the regenerative process is compromised, including necrosis, atrophic non-unions and osteoporosis. To describe the extent of this situation, it is estimated that annually more than 2.2 million patients receive bone defect repairs worldwide, with a cost greater than $2.5 billion just in the United States; this figure is expected to globally double by 2020 due to a variety of factors, including increased life expectancy. The board of the Bone and Joint Decade in 2009 has assessed that half of the people aged over 65, affected by chronic conditions, suffers of joint diseases and that the number of osteoporotic fractures has doubled in the previous 15 years. It should be also pointed out that the worldwide incidence of bone disorders and conditions is increasing in those societies where population ageing is combined with increased obesity and poor physical activity. Shortcomings, limitations, and complications of current clinical treatments for bone repair and regeneration have been reported in different studies. A variety of graft materials are currently used to enhance bone healing, and the relative success of these materials depends on many factors, not only on the specific properties of the graft itself. In addition to its physical properties, to be effective, a grafting material is required to even provide osteoconductive and/or osteoinductive activities. Osteoconduction, the ability of promoting bone growth by allowing bone formation on material’s surface, may suffice in clinical condition of less severe defects, where sufficient quantities and margins of bone exist. Osteoinduction instead, is the capability of promoting de novo bone formation at soft or hard tissue sites, and offers needful advantages for biologic reconstruction of severe situations. Among most commonly used materials there are allografts, cadaveric bone usually obtained from a bone bank, autologous grafts, bone harvested from the patient’s own body, or synthetic ones, often made of hydroxyapatite or other naturally and biocompatible substances. Allografts, mineralized or demineralized, are histocompatible, available in various forms including demineralized bone… Advisors/Committee Members: tutors: M. Del Fabbro, E. Pedrazzini, coordinator: R.L. Weinstein, DEL FABBRO, MASSIMO, WEINSTEIN, ROBERTO LODOVICO.

Subjects/Keywords: Plant Based Pharmaceuticals; Recombinant Proteins; Bone Morphogenetic Proteins; Bone Regeneration; Settore MED/28 - Malattie Odontostomatologiche; Settore BIO/11 - Biologia Molecolare

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ceresoli, V. (2015). PRODUCTION OF HUMAN RECOMBINANT DIFFERENTIATION FACTORS IN TRANSGENIC TOBACCO PLANTS. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/263847

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ceresoli, V.. “PRODUCTION OF HUMAN RECOMBINANT DIFFERENTIATION FACTORS IN TRANSGENIC TOBACCO PLANTS.” 2015. Thesis, Università degli Studi di Milano. Accessed March 05, 2021. http://hdl.handle.net/2434/263847.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ceresoli, V.. “PRODUCTION OF HUMAN RECOMBINANT DIFFERENTIATION FACTORS IN TRANSGENIC TOBACCO PLANTS.” 2015. Web. 05 Mar 2021.

Vancouver:

Ceresoli V. PRODUCTION OF HUMAN RECOMBINANT DIFFERENTIATION FACTORS IN TRANSGENIC TOBACCO PLANTS. [Internet] [Thesis]. Università degli Studi di Milano; 2015. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/2434/263847.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ceresoli V. PRODUCTION OF HUMAN RECOMBINANT DIFFERENTIATION FACTORS IN TRANSGENIC TOBACCO PLANTS. [Thesis]. Università degli Studi di Milano; 2015. Available from: http://hdl.handle.net/2434/263847

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

23. Domingues, Roberta Santos. Efeito da desmineralização ácida da interface enxerto-leito na consolidação de enxertos ósseos autógenos em bloco.

Degree: PhD, Reabilitação Oral, 2013, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/25/25146/tde-02092013-105942/ ;

►

Para testar a hipótese de que a desmineralização in situ das superfícies de contato enxerto-leito, e a forma como o enxerto é estabilizado ao leito,… (more)

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Para testar a hipótese de que a desmineralização in situ das superfícies de contato enxerto-leito, e a forma como o enxerto é estabilizado ao leito, podem influenciar os mecanismos envolvidos na consolidação do enxerto, fragmentos ósseos de 10 mm de diâmetro foram removidos das metáfises proximais tibiais de 36 coelhos (Oryctolagus Cuniculus) e transplantados para uma área adjacente. Na tíbia esquerda dos animais, as superfícies de contato do enxerto e do leito hospedeiro foram desmineralizadas com ácido cítrico pH 1,0 por 3 minutos antes dos enxertos serem fixados ao leito. Na tíbia direita, o transplante do bloco ósseo não foi precedido de desmineralização. Metade dos enxertos foi imobilizada sobre o leito pela superposição de uma membrana não reabsorvível de politetrafluoretileno colada com cianoacrilato ao leito à distância da interface enxerto-leito. A outra metade dos enxertos foi fixada por um parafuso de titânio no centro do enxerto. Assim, foram formados 4 grupos de estudo: membrana (M), membrana + ácido (MA), parafuso (P) e parafuso + ácido (PA). Três animais de cada grupo forneceram espécimes para análise microscópica quantitativa e qualitativa aos 15, 30 e 45 dias de pós-operatório. A análise qualitativa demonstrou que não houve formação óssea na interface em nenhum espécime aos 15 dias e que nos demais períodos, em todos os grupos, a quantidade de tecido ósseo neoformado na interface e seu estágio de maturação aumentaram com o tempo. Ambos os métodos de fixação empregados foram eficientes em manter os enxertos em posição, porém a membrana promoveu menor reabsorção da estrutura do enxerto. A análise quantitativa computadorizada revelou que, aos 30 dias, os grupos MA e PA apresentaram maior área de formação óssea na interface (71,34 ± 12,03%; 56,74 ± 2,15% respectivamente) em relação aos grupos M e P (51,75 ± 11,02%; 43,95 ± 4,05% respectivamente) e superfícies de consolidação óssea mais extensas (93,41 ± 5,95%; 93,73 ± 4,96% respectivamente) do que os grupos sem tratamento ácido (73,49 ± 7,7%; 73,77 ± 11,77% respectivamente para M e P), sendo essas diferenças estatisticamente significantes (p<0,05). Aos 45 dias de pós-operatório, os grupos MA e PA (71,18 ± 8,9%; 58,97 ± 3,97% respectivamente) apresentaram resultados superiores aos grupos M e P (59,78 ± 11,28%; 46,08 ± 3,53% respectivamente) em relação à área de neoformação óssea na interface, porém essa diferença não foi significativa. Concluiu-se que a desmineralização ácida das superfícies contactantes nos enxertos ósseos autógenos em bloco na tíbia de coelhos promoveu a osteogênese na interface enxerto-leito e acelerou a consolidação dos enxertos. Além disso, quando o tratamento ácido foi associado ao uso de membrana como método de fixação, a consolidação e grau de reabsorção óssea foram otimizados.

In order to test the hypothesis that the demineralization ïn situöf contacting surfaces of bone graft/bone bed and the fixation method used for graft stabilization can influence the mechanisms involved in the consolidation of the graft, bone…

Advisors/Committee Members: Rezende, Maria Lucia Rubo de.

Subjects/Keywords: Ácido cítrico; Bone morphogenetic proteins; Bone resorption; Bone transplantation; Citric acid; Demineralization; Desmineralização; Proteínas morfogenéticas ósseas; Reabsorção óssea; Transplante ósseo

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Domingues, R. S. (2013). Efeito da desmineralização ácida da interface enxerto-leito na consolidação de enxertos ósseos autógenos em bloco. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/25/25146/tde-02092013-105942/ ;

Chicago Manual of Style (16^th Edition):

Domingues, Roberta Santos. “Efeito da desmineralização ácida da interface enxerto-leito na consolidação de enxertos ósseos autógenos em bloco.” 2013. Doctoral Dissertation, University of São Paulo. Accessed March 05, 2021. http://www.teses.usp.br/teses/disponiveis/25/25146/tde-02092013-105942/ ;.

MLA Handbook (7^th Edition):

Domingues, Roberta Santos. “Efeito da desmineralização ácida da interface enxerto-leito na consolidação de enxertos ósseos autógenos em bloco.” 2013. Web. 05 Mar 2021.

Vancouver:

Domingues RS. Efeito da desmineralização ácida da interface enxerto-leito na consolidação de enxertos ósseos autógenos em bloco. [Internet] [Doctoral dissertation]. University of São Paulo; 2013. [cited 2021 Mar 05]. Available from: http://www.teses.usp.br/teses/disponiveis/25/25146/tde-02092013-105942/ ;.

Council of Science Editors:

Domingues RS. Efeito da desmineralização ácida da interface enxerto-leito na consolidação de enxertos ósseos autógenos em bloco. [Doctoral Dissertation]. University of São Paulo; 2013. Available from: http://www.teses.usp.br/teses/disponiveis/25/25146/tde-02092013-105942/ ;

UCLA

24. Andrews, Madeline. Bone Morphogenetic Protein Signaling Directs Neural Development in the Dorsal Spinal Cord.

Degree: Neuroscience, 2017, UCLA

URL: http://www.escholarship.org/uc/item/96n7n312

► The dorsal horn of the spinal cord is responsible for integrating, processing and relaying sensory information from the external environment to other parts of the… (more)

Subjects/Keywords: Neurosciences; Developmental biology; Bone morphogenetic proteins; developmental biology; molecular biology; neuroscience; spinal cord

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Andrews, M. (2017). Bone Morphogenetic Protein Signaling Directs Neural Development in the Dorsal Spinal Cord. (Thesis). UCLA. Retrieved from http://www.escholarship.org/uc/item/96n7n312

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Andrews, Madeline. “Bone Morphogenetic Protein Signaling Directs Neural Development in the Dorsal Spinal Cord.” 2017. Thesis, UCLA. Accessed March 05, 2021. http://www.escholarship.org/uc/item/96n7n312.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Andrews, Madeline. “Bone Morphogenetic Protein Signaling Directs Neural Development in the Dorsal Spinal Cord.” 2017. Web. 05 Mar 2021.

Vancouver:

Andrews M. Bone Morphogenetic Protein Signaling Directs Neural Development in the Dorsal Spinal Cord. [Internet] [Thesis]. UCLA; 2017. [cited 2021 Mar 05]. Available from: http://www.escholarship.org/uc/item/96n7n312.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Andrews M. Bone Morphogenetic Protein Signaling Directs Neural Development in the Dorsal Spinal Cord. [Thesis]. UCLA; 2017. Available from: http://www.escholarship.org/uc/item/96n7n312

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

25. Salmeron, Samira. Efeito da desmineralização óssea sobre parâmetros de superfície e sobre o comportamento de pré-osteoblastos em cultura: estudo em microscopia eletrônica de varredura e confocal.

Degree: PhD, Reabilitação Oral, 2015, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/25/25146/tde-03092015-102109/ ;

► A desmineralização óssea superficial tem se demonstrado favorável à consolidação de enxertos e ao comportamento celular, entretanto os mecanismos envolvidos ainda não estão esclarecidos. Os… (more)

▼ A desmineralização óssea superficial tem se demonstrado favorável à consolidação de enxertos e ao comportamento celular, entretanto os mecanismos envolvidos ainda não estão esclarecidos. Os subsídios para o embasamento biológico da desmineralização, proporcionado por publicações anteriores, sugeriram que modificações na superfície óssea teriam influenciado o comportamento de pré-osteoblastos em cultura. Assim, este estudo objetivou comparar o efeito de duas concentrações de ácido cítrico na desmineralização de superfícies ósseas onde foram cultivadas células pré-osteoblásticas (MC3T3-E1), e analisar parâmetros de superfície comparando superfícies desmineralizadas a não desmineralizadas. Setenta amostras ósseas bicorticais foram removidas das calvárias de 35 ratos e divididas em grupos para as análises: 1) Microscopia Eletrônica de Varredura (MEV) para avaliação da área de recobrimento e espessura da camada de células sobre as amostras (n = 15) durante 24, 48 e 72 horas: Grupo AC.10 amostras desmineralizadas por 30 segundos com ácido cítrico 10 %; Grupo AC.50 amostras desmineralizadas por 30 segundos com ácido cítrico 50 %; e Grupo C (controle) amostras não desmineralizadas; 2) Microscopia Confocal para análise da área de expressão e intensidade de fluorescência das BMP-2, -4 e -7: AC.10 seis amostras desmineralizadas conforme item 1); AC.50 seis amostras desmineralizadas conforme item 1); C três amostras não desmineralizadas; 3) Microscopia Confocal para análise da rugosidade superficial média (Ra e Sa): Grupos AC.10 e AC.50 com cinco amostras cada, desmineralizadas conforme o item 1), sendo cada amostra seu próprio controle (análises antes e depois da desmineralização). Também foram avaliadas as distâncias entre picos (P-P) e entre picos e vales (P-V) antes e depois da desmineralização; 4) Microscopia Eletrônica de Varredura / Espectroscopia de Energia Dispersiva (MEV / EDS) para análise da composição superficial: mesmas amostras do item 3) foram avaliadas antes e depois da desmineralização quanto à porcentagem atômica (%A) de carbono, oxigênio, magnésio, fósforo, enxofre e cálcio. Análises estatísticas foram feitas adotando nível de significância de 95 %. Amostras desmineralizadas apresentaram células morfologicamente em estágios mais avançados de diferenciação do que as não desmineralizadas. A área de recobrimento superficial foi significantemente maior após 24 horas de cultura nos grupos teste do que no controle e a espessura da camada de células também foi maior nos grupos teste às 48 e 72 horas. Houve significantemente maior expressão de BMP-2 e -7 nos grupos teste do que no controle e, apenas AC.10 demonstrou maior expressão de BMP-4 do que os demais grupos, sem significância em relação a AC.50. Os parâmetros de superfície Ra e Sa foram inconclusivos, mas P-P e P-V diminuíram consideravelmente após a desmineralização para distâncias compatíveis com superfícies favoráveis à adesão e diferenciação celular. A análise da composição química superficial revelou diminuição da %A de enxofre e magnésio nos… Advisors/Committee Members: Oliveira, Rodrigo Cardoso de, Rezende, Maria Lucia Rubo de.

Subjects/Keywords: Ácido cítrico; Bone morphogenetic proteins; Citric acid; Osteoblastos; Osteoblasts; Proteínas ósseas morfogenéticas

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Salmeron, S. (2015). Efeito da desmineralização óssea sobre parâmetros de superfície e sobre o comportamento de pré-osteoblastos em cultura: estudo em microscopia eletrônica de varredura e confocal. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/25/25146/tde-03092015-102109/ ;

Chicago Manual of Style (16^th Edition):

Salmeron, Samira. “Efeito da desmineralização óssea sobre parâmetros de superfície e sobre o comportamento de pré-osteoblastos em cultura: estudo em microscopia eletrônica de varredura e confocal.” 2015. Doctoral Dissertation, University of São Paulo. Accessed March 05, 2021. http://www.teses.usp.br/teses/disponiveis/25/25146/tde-03092015-102109/ ;.

MLA Handbook (7^th Edition):

Salmeron, Samira. “Efeito da desmineralização óssea sobre parâmetros de superfície e sobre o comportamento de pré-osteoblastos em cultura: estudo em microscopia eletrônica de varredura e confocal.” 2015. Web. 05 Mar 2021.

Vancouver:

Salmeron S. Efeito da desmineralização óssea sobre parâmetros de superfície e sobre o comportamento de pré-osteoblastos em cultura: estudo em microscopia eletrônica de varredura e confocal. [Internet] [Doctoral dissertation]. University of São Paulo; 2015. [cited 2021 Mar 05]. Available from: http://www.teses.usp.br/teses/disponiveis/25/25146/tde-03092015-102109/ ;.

Council of Science Editors:

Salmeron S. Efeito da desmineralização óssea sobre parâmetros de superfície e sobre o comportamento de pré-osteoblastos em cultura: estudo em microscopia eletrônica de varredura e confocal. [Doctoral Dissertation]. University of São Paulo; 2015. Available from: http://www.teses.usp.br/teses/disponiveis/25/25146/tde-03092015-102109/ ;

University of Texas Southwestern Medical Center

26. Sanchez, Angelica. BMP Signaling Regulates Germ Cell Pluripotency, Sexual Differentiation, and Cancer Susceptibility.

Degree: 2016, University of Texas Southwestern Medical Center

URL: http://hdl.handle.net/2152.5/6149

► Testicular germ cell tumors are the most common malignancy found in young men between the ages of 14-40. While these tumors are highly curable with… (more)

▼ Testicular germ cell tumors are the most common malignancy found in young men between the ages of 14-40. While these tumors are highly curable with cisplatin based combined chemotherapies, the treatments come with very detrimental side effects and ultimately fail in up to 15% of patients. These patients have no other avenues of treatment and often times succumb to the disease. Very little is currently known about the biology of the tumors but risk factors highlight possible mechanisms of action. For example, patients with Disorders of Sex Development (DSD) have increased risk for developing malignant germ cell tumors. DSDs manifest at birth and present with atypical gonadal or anatomical sex as well as chromosomal aberrations[1, 2]. Some cases are explained by the presence of chromosomal aberrations, but the cause of many others remains unknown. Such syndromes thus highlight the potential links between germ cell pluripotency, sexual differentiation and cancer susceptibility. A recent GWAS study [3] identified association of BMP7 with testicular dysgenesis syndrome; however the molecular mechanisms behind this association remain unknown. Previously, we described the development of testicular germ cell tumors in zebrafish carrying a mutation in bmpr1bb, a BMP family receptor, and demonstrated that human GCTs have defects in BMP signaling. Here I use this model and next-generation sequencing analysis in a cross-species comparative oncology approach to identify genes and pathways with fundamental importance to the development of the human disease. I further defined the role of specific BMP ligands in mediating germ cell differentiation and identified reciprocal somatic- and germ cell BMP signaling events that regulate germ cell differentiation and maturation. Using genetic crosses to further impair BMP signaling, I found that zebrafish doubly heterozygous for mutations in bmpr1bb and bmp2b, bmp7a or smad5 have profoundly impaired gonadogenesis and altered male:female sex ratios. Affected fish also exhibit markedly abnormal gonadal differentiation, including the presence of undifferentiated gonadal tissue and the occurrence of biphenotypic gonads, as well as greatly increased germ cell tumor susceptibility. Our findings implicate defective BMP pathway signaling as a potential factor in DSDs and GCT susceptibility. Our goals are to identify biological mechanisms that govern germ cell differentiation and to understand how defects in this process cause human disease. Advisors/Committee Members: Castrillon, Diego H., Amatruda, James F., Cobb, Melanie H., Cleaver, Ondine.

Subjects/Keywords: Bone Morphogenetic Proteins; Cell Differentiation; Germ Cells; Neoplasms, Germ Cell and Embryonal; Sex Differentiation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sanchez, A. (2016). BMP Signaling Regulates Germ Cell Pluripotency, Sexual Differentiation, and Cancer Susceptibility. (Thesis). University of Texas Southwestern Medical Center. Retrieved from http://hdl.handle.net/2152.5/6149

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Sanchez, Angelica. “BMP Signaling Regulates Germ Cell Pluripotency, Sexual Differentiation, and Cancer Susceptibility.” 2016. Thesis, University of Texas Southwestern Medical Center. Accessed March 05, 2021. http://hdl.handle.net/2152.5/6149.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Sanchez, Angelica. “BMP Signaling Regulates Germ Cell Pluripotency, Sexual Differentiation, and Cancer Susceptibility.” 2016. Web. 05 Mar 2021.

Vancouver:

Sanchez A. BMP Signaling Regulates Germ Cell Pluripotency, Sexual Differentiation, and Cancer Susceptibility. [Internet] [Thesis]. University of Texas Southwestern Medical Center; 2016. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/2152.5/6149.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Sanchez A. BMP Signaling Regulates Germ Cell Pluripotency, Sexual Differentiation, and Cancer Susceptibility. [Thesis]. University of Texas Southwestern Medical Center; 2016. Available from: http://hdl.handle.net/2152.5/6149

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University College London (University of London)

27. Wardle, Fiona Claire. Regulation of the BMP signalling pathway by BMP1-related metalloproteases.

Degree: PhD, 1998, University College London (University of London)

URL: https://discovery.ucl.ac.uk/id/eprint/10101181/ ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.287477

► Bone Moiphogenetic Proteins (BMPs) 2-8 are members of the TGFβ superfamily of secreted signalling molecules. During embryonic development BMPs are involved in many processes including… (more)

▼ Bone Moiphogenetic Proteins (BMPs) 2-8 are members of the TGFβ superfamily of secreted signalling molecules. During embryonic development BMPs are involved in many processes including cell fate determination, morphogenesis, growth and programmed cell death, all of which are essential for normal development. BMP activity may be regulated in variety of ways. Of particular interest is the finding that three Xenopus proteins. Noggin, Chordin and Follistatin are able to bind BMP4 and prevent it activating its receptor (Piccolo et al., 1996; Zimmerman et al., 1996; Fainsod et al., 1997). Inhibition by Chordin can be alleviated by Xolloid, a BMP1-related metalloprotease that cleaves Chordin (Piccolo et al., 1997). Similarly, in the Drosophila embryo Dpp, a BMP2/4 homologue, is inhibited by the Chordin homologue, Sog, but activated by Tolloid, a BMP 1-related metalloprotease (Marques et al., 1997). Since BMP1-related metalloproteases are present during development of many animal species, an attractive hypothesis is that release from inactive complexes by these metal loproteases is a general mechanism for regulating BMP activity. Mesodermal patterning during Xenopus development, a process known to require BMP activity, is used as a test system to investigate the role of BMP 1-related metalloproteases in BMP signalling. Recent experiments have shown that overexpression of XBMPl and Xolloid partially ventralizes dorsal mesoderm (Goodman et al., 1998). In contrast, expression of the sea urchin BMP 1-related metalloprotease, SpAN, completely ventralizes dorsal mesoderm. This ventralizing activity requires a functional BMP signalling pathway, indicating that these metalloproteases may be activating endogenous BMPs in the Xenopus embryo. In addition, SpAN, XBMP1 and Xolloid inhibit the activity of Chordin and SpAN is also inhibits Noggin. To further test the action of these metalloproteases, putative dominant-negative constructs were made, lacking the metalloprotease domain but retaining the protein-protein interaction domains. These truncated constructs dorsalize ventral mesoderm, consistent with the truncated protein binding its target, but being unable to process it. Finally, two sea urchin TGFβs expressed during early sea urchin development, Univin and suBMP2/4, were tested in the Xenopus embryo and found to be functionally homologous to Vgl and BMP4 respectively, suggesting that similar molecules may act in both sea urchins and Xenopus to pattern the early embryo.

Subjects/Keywords: 572; Bone Morphogenetic Proteins; Cell death

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wardle, F. C. (1998). Regulation of the BMP signalling pathway by BMP1-related metalloproteases. (Doctoral Dissertation). University College London (University of London). Retrieved from https://discovery.ucl.ac.uk/id/eprint/10101181/ ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.287477

Chicago Manual of Style (16^th Edition):

Wardle, Fiona Claire. “Regulation of the BMP signalling pathway by BMP1-related metalloproteases.” 1998. Doctoral Dissertation, University College London (University of London). Accessed March 05, 2021. https://discovery.ucl.ac.uk/id/eprint/10101181/ ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.287477.

MLA Handbook (7^th Edition):

Wardle, Fiona Claire. “Regulation of the BMP signalling pathway by BMP1-related metalloproteases.” 1998. Web. 05 Mar 2021.

Vancouver:

Wardle FC. Regulation of the BMP signalling pathway by BMP1-related metalloproteases. [Internet] [Doctoral dissertation]. University College London (University of London); 1998. [cited 2021 Mar 05]. Available from: https://discovery.ucl.ac.uk/id/eprint/10101181/ ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.287477.

Council of Science Editors:

Wardle FC. Regulation of the BMP signalling pathway by BMP1-related metalloproteases. [Doctoral Dissertation]. University College London (University of London); 1998. Available from: https://discovery.ucl.ac.uk/id/eprint/10101181/ ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.287477

Hong Kong University of Science and Technology

28. Wong, Kwok Hei. Mapping of CRM-1 and LON-1 interaction domains and characterization of their regulatory functions on the BMP pathway.

Degree: 2012, Hong Kong University of Science and Technology

URL: http://repository.ust.hk/ir/Record/1783.1-7663 ; https://doi.org/10.14711/thesis-b1190225 ; http://repository.ust.hk/ir/bitstream/1783.1-7663/1/th_redirect.html

► The control of the body sizes of organisms has long been a topic of interest in developmental biology. In the past decade, Caenorhabditis elegans (C.… (more)

▼ The control of the body sizes of organisms has long been a topic of interest in developmental biology. In the past decade, Caenorhabditis elegans (C. elegans) has been used to reveal the principles that underlie the control of body size. Well-characterized developmental processes of C. elegans facilitate in-depth investigations of the growth regulation that involves many genes. Bone morphogenetic protein (BMP) pathways, a sub-family of the transforming growth factor (TGF)-beta signaling pathways, is one of the major regulatory pathways that control the C. elegans body length. The BMP signal is activated by the binding of DBL-1 to the receptors, transduced to the nucleus of the cell by Smad proteins. The transcription of target genes is regulated by the BMP signal strength, and thus controls the animal growth. Recently, a BMP facilitator, CRM-1, has been found to have putative physical interaction with the product of a target gene of a BMP pathway, LON-1. This observation confers the possibility that LON-1 could regulate the BMP signaling event via physical interaction with CRM-1. In this study, the CRM-1;LON-1 physical interaction is tested and the interaction domains are mapped by a yeast-2-hybrid assay. The SCP-domain on LON-1 and the cysteine-rich (CR) domain on CRM-1 are responsible for this interaction. Moreover, the establishment of a genetic relationship between crm-1 and lon-1 is attempted, yet no clear cut epistatic relationship can be revealed as the crm-1;lon-1 double mutant displays an intermediate phenotype. The molecular function of LON-1 is investigated, and a dominant negative effect cannot be observed by the ectopic expression of lon-1 complementary DNA (cDNA) in the GABA neurons and body wall muscle in wild-type (WT) animals. The results suggest that lon-1 can only perform its function at the hypodermis. Finally, transcriptional reporter lon-1::gfp is generated to investigate the effect of mutations in crm-1 and lon-1. The possible self-regulation of lon-1 by a negative feedback mechanism is revealed and then discussed.

Subjects/Keywords: Bone morphogenetic proteins ; Cellular signal transduction ; Growth – Regulation ; Caenorhabditis elegans – Genetics ; Caenorhabditis elegans – Morphogenesis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wong, K. H. (2012). Mapping of CRM-1 and LON-1 interaction domains and characterization of their regulatory functions on the BMP pathway. (Thesis). Hong Kong University of Science and Technology. Retrieved from http://repository.ust.hk/ir/Record/1783.1-7663 ; https://doi.org/10.14711/thesis-b1190225 ; http://repository.ust.hk/ir/bitstream/1783.1-7663/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Wong, Kwok Hei. “Mapping of CRM-1 and LON-1 interaction domains and characterization of their regulatory functions on the BMP pathway.” 2012. Thesis, Hong Kong University of Science and Technology. Accessed March 05, 2021. http://repository.ust.hk/ir/Record/1783.1-7663 ; https://doi.org/10.14711/thesis-b1190225 ; http://repository.ust.hk/ir/bitstream/1783.1-7663/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Wong, Kwok Hei. “Mapping of CRM-1 and LON-1 interaction domains and characterization of their regulatory functions on the BMP pathway.” 2012. Web. 05 Mar 2021.

Vancouver:

Wong KH. Mapping of CRM-1 and LON-1 interaction domains and characterization of their regulatory functions on the BMP pathway. [Internet] [Thesis]. Hong Kong University of Science and Technology; 2012. [cited 2021 Mar 05]. Available from: http://repository.ust.hk/ir/Record/1783.1-7663 ; https://doi.org/10.14711/thesis-b1190225 ; http://repository.ust.hk/ir/bitstream/1783.1-7663/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Wong KH. Mapping of CRM-1 and LON-1 interaction domains and characterization of their regulatory functions on the BMP pathway. [Thesis]. Hong Kong University of Science and Technology; 2012. Available from: http://repository.ust.hk/ir/Record/1783.1-7663 ; https://doi.org/10.14711/thesis-b1190225 ; http://repository.ust.hk/ir/bitstream/1783.1-7663/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Hong Kong

29. 周立雄. Differential action of bone morphogenetic protein BMP-2 and BMP-7 on nucleus pulposus cells of intervertebral disc.

Degree: 2014, University of Hong Kong

URL: http://hdl.handle.net/10722/209509

► Low back pain (LBP) is associated with intervertebral disc (IVD) degeneration and exerts enormous socioeconomic burdens on the society. The nucleus pulposus (NP) is the… (more)

▼ Low back pain (LBP) is associated with intervertebral disc (IVD) degeneration and exerts enormous socioeconomic burdens on the society. The nucleus pulposus (NP) is the structural and functional core of the IVD, and plays vital roles in its homeostasis. Although the etiology of IVD degeneration is not fully understood, the cellular changes of the NP have been proposed to be associated with degeneration. Conventional management for IVD degeneration primarily targets to relieve LBP and other symptoms without restoring or preserving disc function. Novel therapeutic strategies have emerged with an aim to retard or even reverse disc degeneration. In particular, the use of growth factors, such as the bone morphogenetic proteins (BMP), has received considerable attention due to their anabolic effects on extracellular matrix (ECM) synthesis by NP cells. BMP-2 and BMP-7 are of great interest for their involvement in osteogenesis, chondrogenesis, and development and maintenance of the IVD. To date, the benefits of BMP-2 on disc degeneration are controversial, given the inconsistent findings from animal model studies. The effectiveness of BMP-7 in disc repair, however, has been well demonstrated both in vitro and in vivo. A better understanding of the differences between BMP-2 and BMP-7 regulatory action on NP cells may facilitate future applications of BMP in disc repair/regeneration. This study hypothesized that BMP-2 and BMP-7 act differentially on human NP cells via different signal transduction processes. The differential effect of BMP-2 and BMP-7 was first tested in bovine NP cells using a three-dimensional culture system (alginate beads). Both BMP-2 and BMP-7 enhanced ECM production and phenotypic characteristics of bovine NP cells. Notably, BMP-7 was significantly more potent than BMP-2 in this regard. The effects of BMPs were further tested on non-degenerated (ND-NP) and degenerated (D-NP) human NP cells. The DMMB assay revealed that BMP-7 exerted a superior up-regulatory action on GAG production of D-NP cells compared to BMP-2. Furthermore, the overall response of D-NP cells to BMP-2 and BMP-7 was significantly lower than ND-NP cells. Immunohistochemical staining and quantitative RT-PCR assays demonstrated that D-NP cells possess a more fibroblastic and less chondrocyte-like phenotype than ND-NP cells. At the mRNA level, the BMP receptor BMPR1A was not expressed in D-NP cells. BMP-7, but not BMP-2, induced expression of BMPR1A in D-NP cells. On the other hand, gene expression of selected TGF-β pathway components and hypoxia pathway components were significantly up-regulated by BMP-2 but down-regulated by BMP-7. These findings suggest that D-NP cells can activate differential molecular cascades in response to BMP-2 and BMP-7. In conclusion, this study showed a superior effect of BMP7 in up-regulation of classical BMP signaling components including BMP receptor BMPR1A. The reduced responsiveness of D-NP cells to BMP-2 and BMP-7 stimulation may be related to a different expression pattern of BMP receptors. This…

Subjects/Keywords: Bone morphogenetic proteins; Intervertebral disk - Diseases

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

周立雄. (2014). Differential action of bone morphogenetic protein BMP-2 and BMP-7 on nucleus pulposus cells of intervertebral disc. (Thesis). University of Hong Kong. Retrieved from http://hdl.handle.net/10722/209509

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

周立雄. “Differential action of bone morphogenetic protein BMP-2 and BMP-7 on nucleus pulposus cells of intervertebral disc.” 2014. Thesis, University of Hong Kong. Accessed March 05, 2021. http://hdl.handle.net/10722/209509.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

周立雄. “Differential action of bone morphogenetic protein BMP-2 and BMP-7 on nucleus pulposus cells of intervertebral disc.” 2014. Web. 05 Mar 2021.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Vancouver:

周立雄. Differential action of bone morphogenetic protein BMP-2 and BMP-7 on nucleus pulposus cells of intervertebral disc. [Internet] [Thesis]. University of Hong Kong; 2014. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/10722/209509.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

周立雄. Differential action of bone morphogenetic protein BMP-2 and BMP-7 on nucleus pulposus cells of intervertebral disc. [Thesis]. University of Hong Kong; 2014. Available from: http://hdl.handle.net/10722/209509

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

University of Hong Kong

30. Li, Ruixi. Anti-inflammatory role of bone morphogenetic protein 7 in the diabetic kidney.

Degree: 2015, University of Hong Kong

URL: http://hdl.handle.net/10722/211143

► Diabetic nephropathy (DN) has become the leading cause of end stage renal disease (ESRD) in developed countries, in which over 90% of the diabetes burden… (more)

▼ Diabetic nephropathy (DN) has become the leading cause of end stage renal disease (ESRD) in developed countries, in which over 90% of the diabetes burden is type 2 in origin. Despite the ever advancing knowledge in the pathogenetic mechanisms of DN, a specific treatment is still lacking and management using contemporary approaches or exploiting newly described therapeutic targets are largely unrewarding. BMP-7 has been reported to confer renal protective effects in acute and chronic kidney disease models, but its potential utility in type 2 diabetic nephropathy remains unknown. In this work, therefore, I hypothesized that BMP-7 confers renal protection that will be explore in vitro in AGE-induced tubular epithelial cells, and in vivo in a murine type 2 DN model. Primary human proximal tubular epithelial cells (PTECs) were growth-arrested and exposed to glycated human serum albumin (AGEs) with or without BMP-7. Inhibitors of different signaling pathways were used to dissect the involvement of each pathway. Nine-week-old db/db mice and their db/m littermates underwent uninephrectomy (Unx) or sham operation, and received BMP-7 (300 μg/kg body weight) or vehicle treatment intraperitoneally every other day for 8 weeks before sacrifice. In cultured human PTECs, exposure to AGEs induced overexpression of ICMA-1, MCP-1, IL-8 and IL-6, involving activation of at least p44/42 and p38 MAPK signaling. BMP-7 dose-dependently attenuated AGE-induced up-regulation of ICMA-1, MCP-1, IL-8 and IL-6 at both mRNA and protein levels. Moreover, BMP-7 suppressed AGE-induced p38 and p44/42 MAPK phosphorylation and reactive oxygen species production in PTECs. Compared with vehicle control, Unx db/db mice treated with BMP-7 for 8 weeks had significantly lower urinary albumin-to-creatinine ratio (3549±816.2 μg/mg vs. 8612±2037 μg/mg, p=0.036), serum BUN (33.26±1.09 mg/dL vs. 37.49±0.89 mg/dL, p=0.006), and renal cortical expression of ICMA-1 and MCP-1 at both gene and protein levels. PAS staining of kidney tissue showed less severe tubular damage and interstitial inflammatory cell infiltration in the BMP-7-treated group. In conclusion, this series of experiments demonstrated that BMP-7 attenuates tubular pro-inflammatory responses in diabetic kidney disease by suppressing oxidative stress and multiple signaling pro-inflammatory pathways including p38 and p44/42 MAPK. These observations are largely translated in animals with diabetic kidney inflammation. The potential application of BMP-7 as a therapeutic molecule in diabetic nephropathy warrants further investigation and development.

Subjects/Keywords: Bone morphogenetic proteins; Diabetic nephropathies - Treatment

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Li, R. (2015). Anti-inflammatory role of bone morphogenetic protein 7 in the diabetic kidney. (Thesis). University of Hong Kong. Retrieved from http://hdl.handle.net/10722/211143

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Li, Ruixi. “Anti-inflammatory role of bone morphogenetic protein 7 in the diabetic kidney.” 2015. Thesis, University of Hong Kong. Accessed March 05, 2021. http://hdl.handle.net/10722/211143.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Li, Ruixi. “Anti-inflammatory role of bone morphogenetic protein 7 in the diabetic kidney.” 2015. Web. 05 Mar 2021.

Vancouver:

Li R. Anti-inflammatory role of bone morphogenetic protein 7 in the diabetic kidney. [Internet] [Thesis]. University of Hong Kong; 2015. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/10722/211143.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Li R. Anti-inflammatory role of bone morphogenetic protein 7 in the diabetic kidney. [Thesis]. University of Hong Kong; 2015. Available from: http://hdl.handle.net/10722/211143

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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Open Access Theses and Dissertations