subject:(biotherapeutics)

Texas A&M University

1. Simeon, Rudo Lyndon. Selecting a path against Hepatitis C Virus.

Degree: PhD, Chemical Engineering, 2013, Texas A&M University

URL: http://hdl.handle.net/1969.1/151941

► Hepatitis C virus (HCV) currently affects ~5% of the world’s population and has relatively limited treatment options for infected patients. Genetic suppressor elements (GSE) derived… (more)

▼ Hepatitis C virus (HCV) currently affects ~5% of the world’s population and has relatively limited treatment options for infected patients. Genetic suppressor elements (GSE) derived from a gene or genome of interest can act as transdominant inhibitors of a particular biology function presumably by binding to and blocking an essential interaction surface for protein activity. Taking advantage of hepatoma cell line n4mBid, that supports all stages of the HCV life cycle and strongly report HCV infection by a cell-death phenotype, we developed an iterative selection/enrichment strategy for the identification of GSE against HCV. Using this strategy, a library expressing random fragments of the HCV genome was screened for sequences able to suppress HCV infection. A 244 amino acid gene fragment, B1, was strongly enriched after 5 rounds of selection. B1 has a very high net positive charge of 43 at neutral pH and a high charge-to-mass (kDa) ratio of 1.5. We show that B1 expression specifically inhibits HCV replication, apparently due to its high positive charge. We also show that recombinant positively charged proteins can inhibit HCV infection, when supplied in vitro.In addition, eGFP-fused B1 potently penetrates both adherent and suspension cells with >80% of cells taking up the protein. Importantly, we show that B1 not only facilitates cellular uptake, but allows protein cargo to reach sites of biological relevance. B1 also delivers non-covalently conjugated RNA and DNA across the cell membrane to cytosolic and nuclear sites, with efficiency comparable to commercially available cationic lipid reagents. Our data suggest that B1 utilizes cell-surface glycans and multiple competing endocytic pathways to enter and traffic through cells.During a separate screening carried out in our lab, we identified a TACR3 inhibitor SB 222200 that had significant HCV activity. We go on to show that both TACR1 and TACR3 receptors are expressed in the HCV-permissive Huh 7.5 cell line. We also show that both TACR1 and TACR3 inhibitors significantly inhibit HCV infection. These results point to the potential for TACR1 antagonists in treating patients infected with both HCV and HIV. Advisors/Committee Members: Chen, Zhilei (advisor), Jayaraman, Arul (committee member), Karim, Nazmul (committee member), Leibowitz, Julian (committee member).

Subjects/Keywords: HCV; biomolecules; biotherapeutics; transfection; transduction

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APA (6^th Edition):

Simeon, R. L. (2013). Selecting a path against Hepatitis C Virus. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/151941

Chicago Manual of Style (16^th Edition):

Simeon, Rudo Lyndon. “Selecting a path against Hepatitis C Virus.” 2013. Doctoral Dissertation, Texas A&M University. Accessed January 20, 2021. http://hdl.handle.net/1969.1/151941.

MLA Handbook (7^th Edition):

Simeon, Rudo Lyndon. “Selecting a path against Hepatitis C Virus.” 2013. Web. 20 Jan 2021.

Vancouver:

Simeon RL. Selecting a path against Hepatitis C Virus. [Internet] [Doctoral dissertation]. Texas A&M University; 2013. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/1969.1/151941.

Council of Science Editors:

Simeon RL. Selecting a path against Hepatitis C Virus. [Doctoral Dissertation]. Texas A&M University; 2013. Available from: http://hdl.handle.net/1969.1/151941

Northeastern University

2. Berger, Victoria Lee. Analysis of N-glycans released from proteins of therapeutic and clinical significance using capillary electrophoresis and liquid chromatography coupled to mass spectrometry.

Degree: MS, Department of Chemistry and Chemical Biology, 2013, Northeastern University

URL: http://hdl.handle.net/2047/d20003114

► N-linked glycosylation is a prevalent post-translational modification which modulates the physical, chemical and biological properties of proteins. Glycosylation is important to monitor in both disease… (more)

▼ N-linked glycosylation is a prevalent post-translational modification which modulates the physical, chemical and biological properties of proteins. Glycosylation is important to monitor in both disease and biotherapeutic products.; A growing part of the biopharmaceutical sector are monoclonal antibody (mAb) based therapeutics, typically of the immunoglobulin G (IgG) class which contain a conserved glycosylation site at asparagine-297 in the crystallizable fragment (Fc) region. This site can be occupied by 32 possible glycan structures (in human serum IgG) that affect the function of the molecule. Therefore due to the vast microheterogeneity that can arise, biopharmaceutical products must be analyzed during and after manufacturing to verify the glycan chains or monosaccharides present. One approach to this analysis is by using capillary zone electrophoresis (CZE) with laser induced fluorescence (LIF) to monitor the abundance of individual monosaccharides from released and hydrolyzed N-glycan chains on the mAb biotherapeutic. This quantitative approach was applied to nine biotherapeutic monoclonal antibodies. The original procedure, as obtained from the literature, was further optimized, using sample clean-up protocols and dilution, to reduce noise present due to the sensitivity of the fluorescence detector and ultimately to improve the separation of the monosaccharide peaks. The resulting data showed that the therapeutics contained high levels of fucosylation with variable levels of galactosylation consistent with human glycosylation.; To investigate alterations in sialylated glycans that are associated with disease, a previously developed fluoride-mediated negative ion microfluidic chip LC-MS method can be applied; however, the glycosidic bond between the sialic acid and underlying glycan is labile and easily lost during mass spectrometric ionization. To minimize the charge on the sialic acids and therefore, to enable the application of our fluoride-mediated method, a charge neutralization reaction such as methylation or methyl amidation of the carboxylic acid can be used. A variety of reagents were tested to methylate the carboxylic acid on the sialic acid; however, none of the methylation procedures showed completed conversion of the carboxylic acids to methyl esters. A methyl amidation approach was also undertaken with (7-azabenzotriazol-1-yloxy)trispyrrolidinophosphonium hexafluorophosphate (PyAOP). This approach proved to be promising as it showed complete conversion for both linkages (α(2,3) and α(2,6)) on disialyllacto-N-tetraose, a disialylated milk sugar. When the procedure was further applied to a disialylated, complex N-glycan, an incomplete reaction was seen indicating the further need for optimization of the reaction conditions. After further optimization, PyAOP will be applied to investigate glycosylation on alpha-1 acid glycoprotein, a heavily sialylated glycoprotein involved in immune response and implicated in disease progression.

Subjects/Keywords: biotherapeutics; capillary electrophoresis; glycans; mass spectrometry; Chemistry; Medicinal-Pharmaceutical Chemistry

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APA (6^th Edition):

Berger, V. L. (2013). Analysis of N-glycans released from proteins of therapeutic and clinical significance using capillary electrophoresis and liquid chromatography coupled to mass spectrometry. (Masters Thesis). Northeastern University. Retrieved from http://hdl.handle.net/2047/d20003114

Chicago Manual of Style (16^th Edition):

Berger, Victoria Lee. “Analysis of N-glycans released from proteins of therapeutic and clinical significance using capillary electrophoresis and liquid chromatography coupled to mass spectrometry.” 2013. Masters Thesis, Northeastern University. Accessed January 20, 2021. http://hdl.handle.net/2047/d20003114.

MLA Handbook (7^th Edition):

Berger, Victoria Lee. “Analysis of N-glycans released from proteins of therapeutic and clinical significance using capillary electrophoresis and liquid chromatography coupled to mass spectrometry.” 2013. Web. 20 Jan 2021.

Vancouver:

Berger VL. Analysis of N-glycans released from proteins of therapeutic and clinical significance using capillary electrophoresis and liquid chromatography coupled to mass spectrometry. [Internet] [Masters thesis]. Northeastern University; 2013. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/2047/d20003114.

Council of Science Editors:

Berger VL. Analysis of N-glycans released from proteins of therapeutic and clinical significance using capillary electrophoresis and liquid chromatography coupled to mass spectrometry. [Masters Thesis]. Northeastern University; 2013. Available from: http://hdl.handle.net/2047/d20003114

3. Christiaen, Steven. Exploring the role of quorum sensing and quorum sensing inhibition in the biotherapeutic potential of bacteria.

Degree: 2014, Ghent University

URL: http://hdl.handle.net/1854/LU-4232258

► The involvement of quorum sensing (QS) in several pathogenic bacteria to control biofilm formation and the expression of their virulence factors makes interfering with QS… (more)

Subjects/Keywords: Medicine and Health Sciences; quorum sensing; biotherapeutics; quorum quenching; probiotics

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APA (6^th Edition):

Christiaen, S. (2014). Exploring the role of quorum sensing and quorum sensing inhibition in the biotherapeutic potential of bacteria. (Thesis). Ghent University. Retrieved from http://hdl.handle.net/1854/LU-4232258

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Christiaen, Steven. “Exploring the role of quorum sensing and quorum sensing inhibition in the biotherapeutic potential of bacteria.” 2014. Thesis, Ghent University. Accessed January 20, 2021. http://hdl.handle.net/1854/LU-4232258.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Christiaen, Steven. “Exploring the role of quorum sensing and quorum sensing inhibition in the biotherapeutic potential of bacteria.” 2014. Web. 20 Jan 2021.

Vancouver:

Christiaen S. Exploring the role of quorum sensing and quorum sensing inhibition in the biotherapeutic potential of bacteria. [Internet] [Thesis]. Ghent University; 2014. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/1854/LU-4232258.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Christiaen S. Exploring the role of quorum sensing and quorum sensing inhibition in the biotherapeutic potential of bacteria. [Thesis]. Ghent University; 2014. Available from: http://hdl.handle.net/1854/LU-4232258

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

4. Clausen Lind, Andrea. Exploring the mycobiota for the treatment of gut-related diseases .

Degree: Chalmers tekniska högskola / Institutionen för biologi och bioteknik, 2020, Chalmers University of Technology

URL: http://hdl.handle.net/20.500.12380/300767

► Our gut harbors trillions of microorganisms that form a bridge between our diet and whole-body metabolism. This collection of microorganisms, referred to as the gut… (more)

Subjects/Keywords: Yeast Probiotics; Live biotherapeutics; Phenylketonuria; Mycobiome; Metagenomic analysis; Inflammatory Bowel Disease

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APA (6^th Edition):

Clausen Lind, A. (2020). Exploring the mycobiota for the treatment of gut-related diseases . (Thesis). Chalmers University of Technology. Retrieved from http://hdl.handle.net/20.500.12380/300767

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Clausen Lind, Andrea. “Exploring the mycobiota for the treatment of gut-related diseases .” 2020. Thesis, Chalmers University of Technology. Accessed January 20, 2021. http://hdl.handle.net/20.500.12380/300767.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Clausen Lind, Andrea. “Exploring the mycobiota for the treatment of gut-related diseases .” 2020. Web. 20 Jan 2021.

Vancouver:

Clausen Lind A. Exploring the mycobiota for the treatment of gut-related diseases . [Internet] [Thesis]. Chalmers University of Technology; 2020. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/20.500.12380/300767.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Clausen Lind A. Exploring the mycobiota for the treatment of gut-related diseases . [Thesis]. Chalmers University of Technology; 2020. Available from: http://hdl.handle.net/20.500.12380/300767

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Manchester

5. Nuhu, Mariam. Protein-protein interactions and aggregation in biotherapeutics.

Degree: 2015, University of Manchester

URL: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:277397

► Protein aggregation is a frequently cited problem during the development of liquid protein formulations, which is especially problematic since each protein exhibits different aggregation behaviour.… (more)

Subjects/Keywords: protein aggregation; protein-protein interaction; excipients; formulation; biotherapeutics

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APA (6^th Edition):

Nuhu, M. (2015). Protein-protein interactions and aggregation in biotherapeutics. (Doctoral Dissertation). University of Manchester. Retrieved from http://www.manchester.ac.uk/escholar/uk-ac-man-scw:277397

Chicago Manual of Style (16^th Edition):

Nuhu, Mariam. “Protein-protein interactions and aggregation in biotherapeutics.” 2015. Doctoral Dissertation, University of Manchester. Accessed January 20, 2021. http://www.manchester.ac.uk/escholar/uk-ac-man-scw:277397.

MLA Handbook (7^th Edition):

Nuhu, Mariam. “Protein-protein interactions and aggregation in biotherapeutics.” 2015. Web. 20 Jan 2021.

Vancouver:

Nuhu M. Protein-protein interactions and aggregation in biotherapeutics. [Internet] [Doctoral dissertation]. University of Manchester; 2015. [cited 2021 Jan 20]. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:277397.

Council of Science Editors:

Nuhu M. Protein-protein interactions and aggregation in biotherapeutics. [Doctoral Dissertation]. University of Manchester; 2015. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:277397

Brigham Young University

6. Salehi, Sayed Mohammad. Engineering Cell-free Protein Synthesis Technology for Codon Reassignment, Biotherapeutics Production using Just-add-Water System, and Biosensing Endocrine Disrupting Compounds.

Degree: PhD, 2017, Brigham Young University

URL: https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=7711&context=etd

► Cell-free protein synthesis is an emerging technology that has many applications. The open nature of this system makes it a compelling technology that can be… (more)

▼ Cell-free protein synthesis is an emerging technology that has many applications. The open nature of this system makes it a compelling technology that can be manipulated to answer many needs that are unavailable in other systems. This dissertation reports on engineering this technology for: 1) sense codon emancipation for incorporation of multiple unnatural amino acids; 2) expressing a hard-to-express anticancer biotherapeutic and introducing a just-add-water system; 3) a biosensing ligand that interacts with nuclear hormone receptors. Emancipating sense codons toward a minimized genetic code is of significant interest to science and engineering. A promising approach to sense codon emancipation is the targeted in vitro removal of native tRNA. Here we introduce a new in-vitro or "cell-free" approach to emancipate sense codons via efficient and affordable degradation of endogenous tRNA using RNase-coated superparamagnetic beads. The presented method removes greater than 99% of tRNA in cell lysates, while preserving cell-free protein synthesis activity. The resulting tRNA-depleted lysate is compatible with in vitro-transcribed synthetic tRNA for the production of peptides and proteins. Biotherapeutics have many promising applications, such as anti-cancer treatments, immune suppression, and vaccines. However, due to their biological nature, some biotherapeutics can be challenging to rapidly express and screen for activity through traditional recombinant methods. In this work, we demonstrate the use of cell-free systems for the expression and direct screening of the difficult-to-express cytotoxic protein onconase. Using cell-free systems, onconase can be rapidly expressed in soluble, active form. Furthermore, the open nature of the reaction environment allows for direct and immediate downstream characterization without the need of purification. Also, we report the ability of a "just-add-water" lyophilized cell-fee system to produce onconase. Here we introduce a Rapid Adaptable Portable In-vitro Detection biosensor platform (RAPID) for detecting ligands that interact with nuclear hormone receptors (NHRs). The biosensor is based on an engineered, allosterically-activated fusion protein, which contains the ligand binding domain from a target NHR. The presented RAPID biosensor platform is significantly faster and less labor intensive than commonly available technologies, making it a promising tool for detecting environmental EDC contamination and screening potential NHR-targeted pharmaceuticals.

Subjects/Keywords: Sayed Mohammad Amin Salehi; cell-free protein synthesis; codon emancipation; cancer biotherapeutics; endocrine disrupting compounds; nuclear hormone receptors; biosensor; Chemical Engineering

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APA (6^th Edition):

Salehi, S. M. (2017). Engineering Cell-free Protein Synthesis Technology for Codon Reassignment, Biotherapeutics Production using Just-add-Water System, and Biosensing Endocrine Disrupting Compounds. (Doctoral Dissertation). Brigham Young University. Retrieved from https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=7711&context=etd

Chicago Manual of Style (16^th Edition):

Salehi, Sayed Mohammad. “Engineering Cell-free Protein Synthesis Technology for Codon Reassignment, Biotherapeutics Production using Just-add-Water System, and Biosensing Endocrine Disrupting Compounds.” 2017. Doctoral Dissertation, Brigham Young University. Accessed January 20, 2021. https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=7711&context=etd.

MLA Handbook (7^th Edition):

Salehi, Sayed Mohammad. “Engineering Cell-free Protein Synthesis Technology for Codon Reassignment, Biotherapeutics Production using Just-add-Water System, and Biosensing Endocrine Disrupting Compounds.” 2017. Web. 20 Jan 2021.

Vancouver:

Salehi SM. Engineering Cell-free Protein Synthesis Technology for Codon Reassignment, Biotherapeutics Production using Just-add-Water System, and Biosensing Endocrine Disrupting Compounds. [Internet] [Doctoral dissertation]. Brigham Young University; 2017. [cited 2021 Jan 20]. Available from: https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=7711&context=etd.

Council of Science Editors:

Salehi SM. Engineering Cell-free Protein Synthesis Technology for Codon Reassignment, Biotherapeutics Production using Just-add-Water System, and Biosensing Endocrine Disrupting Compounds. [Doctoral Dissertation]. Brigham Young University; 2017. Available from: https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=7711&context=etd

7. RYAN HARYADI. THE ISOLATION CHARACTERISATION AND APPLICATION OF CHO GLYCOSYLATION MUTANT CELL LINES IN BIOTHERAPEUTICS.

Degree: 2019, National University of Singapore

URL: https://scholarbank.nus.edu.sg/handle/10635/155578

Subjects/Keywords: CHO; n-glycosylation; mutants; biotherapeutics; o-glycosylation; glycans

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APA (6^th Edition):

HARYADI, R. (2019). THE ISOLATION CHARACTERISATION AND APPLICATION OF CHO GLYCOSYLATION MUTANT CELL LINES IN BIOTHERAPEUTICS. (Thesis). National University of Singapore. Retrieved from https://scholarbank.nus.edu.sg/handle/10635/155578

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

HARYADI, RYAN. “THE ISOLATION CHARACTERISATION AND APPLICATION OF CHO GLYCOSYLATION MUTANT CELL LINES IN BIOTHERAPEUTICS.” 2019. Thesis, National University of Singapore. Accessed January 20, 2021. https://scholarbank.nus.edu.sg/handle/10635/155578.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

HARYADI, RYAN. “THE ISOLATION CHARACTERISATION AND APPLICATION OF CHO GLYCOSYLATION MUTANT CELL LINES IN BIOTHERAPEUTICS.” 2019. Web. 20 Jan 2021.

Vancouver:

HARYADI R. THE ISOLATION CHARACTERISATION AND APPLICATION OF CHO GLYCOSYLATION MUTANT CELL LINES IN BIOTHERAPEUTICS. [Internet] [Thesis]. National University of Singapore; 2019. [cited 2021 Jan 20]. Available from: https://scholarbank.nus.edu.sg/handle/10635/155578.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

HARYADI R. THE ISOLATION CHARACTERISATION AND APPLICATION OF CHO GLYCOSYLATION MUTANT CELL LINES IN BIOTHERAPEUTICS. [Thesis]. National University of Singapore; 2019. Available from: https://scholarbank.nus.edu.sg/handle/10635/155578

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

8. Čaval, Tomislav. Mass Spectrometric Exploration of the GlycoUniverse.

Degree: 2019, University Utrecht

URL: https://dspace.library.uu.nl/handle/1874/386312 ; URN:NBN:NL:UI:10-1874-386312 ; 1874/386312 ; urn:isbn:9789039372104 ; URN:NBN:NL:UI:10-1874-386312 ; https://dspace.library.uu.nl/handle/1874/386312

► Currently over 80% of biological drugs on the market are modified by sugar residues called glycans. Presence of these glycans on each of these molecules… (more)

Subjects/Keywords: Native mass spectrometry; Glycoproteomics; Glycosylation; Biotherapeutics; Glycoengineering

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APA (6^th Edition):

Čaval, T. (2019). Mass Spectrometric Exploration of the GlycoUniverse. (Doctoral Dissertation). University Utrecht. Retrieved from https://dspace.library.uu.nl/handle/1874/386312 ; URN:NBN:NL:UI:10-1874-386312 ; 1874/386312 ; urn:isbn:9789039372104 ; URN:NBN:NL:UI:10-1874-386312 ; https://dspace.library.uu.nl/handle/1874/386312

Chicago Manual of Style (16^th Edition):

Čaval, Tomislav. “Mass Spectrometric Exploration of the GlycoUniverse.” 2019. Doctoral Dissertation, University Utrecht. Accessed January 20, 2021. https://dspace.library.uu.nl/handle/1874/386312 ; URN:NBN:NL:UI:10-1874-386312 ; 1874/386312 ; urn:isbn:9789039372104 ; URN:NBN:NL:UI:10-1874-386312 ; https://dspace.library.uu.nl/handle/1874/386312.

MLA Handbook (7^th Edition):

Čaval, Tomislav. “Mass Spectrometric Exploration of the GlycoUniverse.” 2019. Web. 20 Jan 2021.

Vancouver:

Čaval T. Mass Spectrometric Exploration of the GlycoUniverse. [Internet] [Doctoral dissertation]. University Utrecht; 2019. [cited 2021 Jan 20]. Available from: https://dspace.library.uu.nl/handle/1874/386312 ; URN:NBN:NL:UI:10-1874-386312 ; 1874/386312 ; urn:isbn:9789039372104 ; URN:NBN:NL:UI:10-1874-386312 ; https://dspace.library.uu.nl/handle/1874/386312.

Council of Science Editors:

Čaval T. Mass Spectrometric Exploration of the GlycoUniverse. [Doctoral Dissertation]. University Utrecht; 2019. Available from: https://dspace.library.uu.nl/handle/1874/386312 ; URN:NBN:NL:UI:10-1874-386312 ; 1874/386312 ; urn:isbn:9789039372104 ; URN:NBN:NL:UI:10-1874-386312 ; https://dspace.library.uu.nl/handle/1874/386312

9. King, Chris. Computational Design of Protein Therapeutics with Reduced Immunogenicity through Structural Modeling of Protein Interactions.

Degree: PhD, 2014, University of Washington

URL: http://hdl.handle.net/1773/25382

► Proteins possess huge potential as therapeutic agents for the control and modulation of human physiology. Protein interactions regulate most physiological processes, mediating the connection between… (more)

Subjects/Keywords: biotherapeutics; deimmunization; machine learning; molecular modeling; protein design; Biochemistry; Bioinformatics; Biophysics; biological chemistry

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

King, C. (2014). Computational Design of Protein Therapeutics with Reduced Immunogenicity through Structural Modeling of Protein Interactions. (Doctoral Dissertation). University of Washington. Retrieved from http://hdl.handle.net/1773/25382

Chicago Manual of Style (16^th Edition):

King, Chris. “Computational Design of Protein Therapeutics with Reduced Immunogenicity through Structural Modeling of Protein Interactions.” 2014. Doctoral Dissertation, University of Washington. Accessed January 20, 2021. http://hdl.handle.net/1773/25382.

MLA Handbook (7^th Edition):

King, Chris. “Computational Design of Protein Therapeutics with Reduced Immunogenicity through Structural Modeling of Protein Interactions.” 2014. Web. 20 Jan 2021.

Vancouver:

King C. Computational Design of Protein Therapeutics with Reduced Immunogenicity through Structural Modeling of Protein Interactions. [Internet] [Doctoral dissertation]. University of Washington; 2014. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/1773/25382.

Council of Science Editors:

King C. Computational Design of Protein Therapeutics with Reduced Immunogenicity through Structural Modeling of Protein Interactions. [Doctoral Dissertation]. University of Washington; 2014. Available from: http://hdl.handle.net/1773/25382

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