subject:(biochemistry)

1. Iyer Mani Sankaran, Siva Sankari. Discovery and analysis of iron export and iron import mechanisms of Bradyrhizobium japonicum and their roles in managing stress responses.

Degree: 2016, State University of New York at Buffalo

URL: http://pqdtopen.proquest.com/#viewpdf?dispub=10127704

► Nutritional iron acquisition by bacteria is well described, but almost nothing is known about bacterial iron export. Here, we show that Bradyrhizobium japonicum… (more)

▼ Nutritional iron acquisition by bacteria is well described, but almost nothing is known about bacterial iron export. Here, we show that Bradyrhizobium japonicum MbfA (Blr7895) is an inner membrane protein expressed in cells specifically under high iron conditions. An mbfA deletion mutant is severely defective in iron export activity, contains >2-fold more intracellular iron than the parent strain, and displays an aberrant iron-dependent gene expression phenotype. The findings suggest that iron export plays an important role in bacterial iron homeostasis, and MbfA is responsible for the iron export activity of B. japonicum. The N-terminal Ferritin like domain (FLD) of MbfA is localized to the cytoplasmic side of the inner membrane and is required for export activity. Purified FLD is a dimer in solution implying that MbfA functions as a dimer. An mbfA mutant is sensitive to short term exposure to high levels iron or H₂O₂ but not when grown in elevated iron media, suggesting a stress response adaptation. The bfr gene encodes the iron storage protein bacterioferritin. An mbfA bfr double mutant showed a loss of stress adaptation, and had a severe growth phenotype in high iron media. The double mutant exhibits elevated intracellular iron content than the wild type, and displays aberrant gene expression even when grown in relatively low iron media. These results suggest that MbfA and Bfr work in concert to manage iron and oxidative stresses. In addition, the need for iron detoxification is not limited to extreme environments, but is also required for normal cellular function. B. japonicum cannot make siderophores for acquisition of iron in aerobic environments. The mechanism of iron uptake in the absence of xenosiderophores is unknown. Exploiting the synthetic lethal phenotype of the mbfA bfr double mutant, we identified suppressor strains that can grow in high iron concentrations. The suppressor strains harbor loss of function mutations in the feoAB operon, which is a ferrous iron transport system. Interestingly, FeoAB system is required for ferric iron utilization and is required for high affinity uptake of both ferric and ferrous iron by B. japonicum. feoB and feoA incited small, poorly developed, non-nitrogen fixing nodules on soybean plants suggesting the requirement of FeoAB system for establishment of symbiosis. A suppressor strain harboring a Glu-40 to Lys mutation in FeoA (feoAE40K ) has diminished but measurable iron uptake activity in free living cells. It elicited nitrogen fixing nodules on soybean but the bacteroids in the nodules displayed lower iron uptake activity compared to wildtype bacteroids. This strongly suggests that the FeoAB transport system is involved in iron uptake within symbiotic bacteroids. Thus our results indicate that B. japonicum employs a single iron transporter to adapt to diverse environmental conditions.

Subjects/Keywords: Biochemistry

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Iyer Mani Sankaran, S. S. (2016). Discovery and analysis of iron export and iron import mechanisms of Bradyrhizobium japonicum and their roles in managing stress responses. (Thesis). State University of New York at Buffalo. Retrieved from http://pqdtopen.proquest.com/#viewpdf?dispub=10127704

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Iyer Mani Sankaran, Siva Sankari. “Discovery and analysis of iron export and iron import mechanisms of Bradyrhizobium japonicum and their roles in managing stress responses.” 2016. Thesis, State University of New York at Buffalo. Accessed February 27, 2021. http://pqdtopen.proquest.com/#viewpdf?dispub=10127704.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Iyer Mani Sankaran, Siva Sankari. “Discovery and analysis of iron export and iron import mechanisms of Bradyrhizobium japonicum and their roles in managing stress responses.” 2016. Web. 27 Feb 2021.

Vancouver:

Iyer Mani Sankaran SS. Discovery and analysis of iron export and iron import mechanisms of Bradyrhizobium japonicum and their roles in managing stress responses. [Internet] [Thesis]. State University of New York at Buffalo; 2016. [cited 2021 Feb 27]. Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=10127704.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Iyer Mani Sankaran SS. Discovery and analysis of iron export and iron import mechanisms of Bradyrhizobium japonicum and their roles in managing stress responses. [Thesis]. State University of New York at Buffalo; 2016. Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=10127704

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

2. Alhewaitey, Anaif. Interactions of aurein with model membranes and antimalarials.

Degree: 2016, Tennessee State University

URL: http://pqdtopen.proquest.com/#viewpdf?dispub=10119062

► Aurein is a cationic antimicrobial peptide, rich in phenylalanine residues. Although the peptide has been extensively studied, its mechanism of action is not fully… (more)

▼ Aurein is a cationic antimicrobial peptide, rich in phenylalanine residues. Although the peptide has been extensively studied, its mechanism of action is not fully understood and has not been established. This project is focused on studying the interactions of aurein with model biological membranes and antimalarials using Fourier Transform Infrared (FTIR), fluorescence, dynamic light scattering (DLS), atomic force microscopy (AFM), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) techniques. FTIR data revealed conformational changes to the secondary structure of the peptide in the presence of the model membranes. The strongest interactions of aurein were found with DOPC and lipid raft systems. Fluorescence data revealed some differences in the mechanism of interaction between aurein and lipid rafts. Topographical analysis was performed using Atomic force microscopy (AFM). AFM images of the peptide with its lipid rafts showed a change in surface roughness suggesting a different mechanism of interaction. DLS data in agreement with FTIR confirmed that aurein interacts differently with the lipid rafts. The results gathered from this study provided new insights on the interaction of aurein. On the other hand, drug-drug interaction issues continue to present a major dilemma for the clinician caring for complex patients such as those infected with infectious disease. This study has examined the interaction of aurein with quinine, primaquine, and chloroquine. Significant interactions between aurein and antimalarials occured at a higher concentration of antimalarials. Interactions between aurein and antimalarials reveal a strong interaction between aurein and primaquine. Interactions between aurein and quinine or chloroquine were found to be weak and negligible. FTIR, TGA, and DSC may be used in a complementary way to gain insights into the possible drug-drug interactions involving aurein. These studies are needed to initiate in vivo controlled interaction studies between antibiotics and antimalarials.

Subjects/Keywords: Biochemistry

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Alhewaitey, A. (2016). Interactions of aurein with model membranes and antimalarials. (Thesis). Tennessee State University. Retrieved from http://pqdtopen.proquest.com/#viewpdf?dispub=10119062

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Alhewaitey, Anaif. “Interactions of aurein with model membranes and antimalarials.” 2016. Thesis, Tennessee State University. Accessed February 27, 2021. http://pqdtopen.proquest.com/#viewpdf?dispub=10119062.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Alhewaitey, Anaif. “Interactions of aurein with model membranes and antimalarials.” 2016. Web. 27 Feb 2021.

Vancouver:

Alhewaitey A. Interactions of aurein with model membranes and antimalarials. [Internet] [Thesis]. Tennessee State University; 2016. [cited 2021 Feb 27]. Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=10119062.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Alhewaitey A. Interactions of aurein with model membranes and antimalarials. [Thesis]. Tennessee State University; 2016. Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=10119062

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

3. Alattas, Noor Abdulrahman S. Polysaccharide-mediated formation of pigments from serotonin.

Degree: 2016, Tennessee State University

URL: http://pqdtopen.proquest.com/#viewpdf?dispub=10119066

► As a continuation of the research on the pigment formation from catecholamines, we studied the polysaccharide-mediated oxidation of serotonin and other 5-hydroxy indoles into… (more)

Subjects/Keywords: Biochemistry

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Alattas, N. A. S. (2016). Polysaccharide-mediated formation of pigments from serotonin. (Thesis). Tennessee State University. Retrieved from http://pqdtopen.proquest.com/#viewpdf?dispub=10119066

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Alattas, Noor Abdulrahman S. “Polysaccharide-mediated formation of pigments from serotonin.” 2016. Thesis, Tennessee State University. Accessed February 27, 2021. http://pqdtopen.proquest.com/#viewpdf?dispub=10119066.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Alattas, Noor Abdulrahman S. “Polysaccharide-mediated formation of pigments from serotonin.” 2016. Web. 27 Feb 2021.

Vancouver:

Alattas NAS. Polysaccharide-mediated formation of pigments from serotonin. [Internet] [Thesis]. Tennessee State University; 2016. [cited 2021 Feb 27]. Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=10119066.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Alattas NAS. Polysaccharide-mediated formation of pigments from serotonin. [Thesis]. Tennessee State University; 2016. Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=10119066

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

4. Fochesato, Lee-Anne. Small Molecular Weight Modulators of Apoptosis and Senescence.

Degree: MA, Chemistry and Biochemistry, 2011, National Library of Canada

URL: http://scholar.uwindsor.ca/etd/70

► ABSTRACT Age-related neurodegenerative diseases are a major problem in industrialized nations, and affect millions of people worldwide. The pro-apoptotic protein Bax plays a crucial part… (more)

Subjects/Keywords: Biochemistry.

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Fochesato, L. (2011). Small Molecular Weight Modulators of Apoptosis and Senescence. (Masters Thesis). National Library of Canada. Retrieved from http://scholar.uwindsor.ca/etd/70

Chicago Manual of Style (16^th Edition):

Fochesato, Lee-Anne. “Small Molecular Weight Modulators of Apoptosis and Senescence.” 2011. Masters Thesis, National Library of Canada. Accessed February 27, 2021. http://scholar.uwindsor.ca/etd/70.

MLA Handbook (7^th Edition):

Fochesato, Lee-Anne. “Small Molecular Weight Modulators of Apoptosis and Senescence.” 2011. Web. 27 Feb 2021.

Vancouver:

Fochesato L. Small Molecular Weight Modulators of Apoptosis and Senescence. [Internet] [Masters thesis]. National Library of Canada; 2011. [cited 2021 Feb 27]. Available from: http://scholar.uwindsor.ca/etd/70.

Council of Science Editors:

Fochesato L. Small Molecular Weight Modulators of Apoptosis and Senescence. [Masters Thesis]. National Library of Canada; 2011. Available from: http://scholar.uwindsor.ca/etd/70

5. Pricop, Ionut. Unraveling the RNAi-like Activity in Toxoplasma Gondii.

Degree: MS, Chemistry and Biochemistry, 2009, National Library of Canada

URL: http://scholar.uwindsor.ca/etd/308

► Ribonuclease activity within the RNAi mechanism has been thoroughly described in model organisms such as Caenorhabditis elegans. However, RNAi is still largely a mystery in… (more)

Subjects/Keywords: Biochemistry.

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Pricop, I. (2009). Unraveling the RNAi-like Activity in Toxoplasma Gondii. (Masters Thesis). National Library of Canada. Retrieved from http://scholar.uwindsor.ca/etd/308

Chicago Manual of Style (16^th Edition):

Pricop, Ionut. “Unraveling the RNAi-like Activity in Toxoplasma Gondii.” 2009. Masters Thesis, National Library of Canada. Accessed February 27, 2021. http://scholar.uwindsor.ca/etd/308.

MLA Handbook (7^th Edition):

Pricop, Ionut. “Unraveling the RNAi-like Activity in Toxoplasma Gondii.” 2009. Web. 27 Feb 2021.

Vancouver:

Pricop I. Unraveling the RNAi-like Activity in Toxoplasma Gondii. [Internet] [Masters thesis]. National Library of Canada; 2009. [cited 2021 Feb 27]. Available from: http://scholar.uwindsor.ca/etd/308.

Council of Science Editors:

Pricop I. Unraveling the RNAi-like Activity in Toxoplasma Gondii. [Masters Thesis]. National Library of Canada; 2009. Available from: http://scholar.uwindsor.ca/etd/308

6. Franklin, Norah. Regulation of MTMR2 by Phosphorylation: Subcellular Characterization and Functional Role in Vesicular Trafficking.

Degree: MA, Chemistry and Biochemistry, 2011, National Library of Canada

URL: http://scholar.uwindsor.ca/etd/71

► MTMR2 is an active member of the MTM family of inositol lipid phosphatases and the physiological substrates for MTMR2 play key roles in endosomal trafficking.… (more)

Subjects/Keywords: Biochemistry.

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Franklin, N. (2011). Regulation of MTMR2 by Phosphorylation: Subcellular Characterization and Functional Role in Vesicular Trafficking. (Masters Thesis). National Library of Canada. Retrieved from http://scholar.uwindsor.ca/etd/71

Chicago Manual of Style (16^th Edition):

Franklin, Norah. “Regulation of MTMR2 by Phosphorylation: Subcellular Characterization and Functional Role in Vesicular Trafficking.” 2011. Masters Thesis, National Library of Canada. Accessed February 27, 2021. http://scholar.uwindsor.ca/etd/71.

MLA Handbook (7^th Edition):

Franklin, Norah. “Regulation of MTMR2 by Phosphorylation: Subcellular Characterization and Functional Role in Vesicular Trafficking.” 2011. Web. 27 Feb 2021.

Vancouver:

Franklin N. Regulation of MTMR2 by Phosphorylation: Subcellular Characterization and Functional Role in Vesicular Trafficking. [Internet] [Masters thesis]. National Library of Canada; 2011. [cited 2021 Feb 27]. Available from: http://scholar.uwindsor.ca/etd/71.

Council of Science Editors:

Franklin N. Regulation of MTMR2 by Phosphorylation: Subcellular Characterization and Functional Role in Vesicular Trafficking. [Masters Thesis]. National Library of Canada; 2011. Available from: http://scholar.uwindsor.ca/etd/71

7. Kaur, Harmanpreet. DEVELOPMENT AND VALIDATION OF A FLOW DEVICE TO STUDY PLATELET FUNCTION IN VITRO AND ELUCIDATING THE ROLE OF THYMOSIN β 4 IN VARIOUS PHYSIOLOGICAL PROCESSES.

Degree: PhD, Chemistry and Biochemistry, 2011, National Library of Canada

URL: http://scholar.uwindsor.ca/etd/393

► Parallel plate flow chambers, simulating in vivo fluid shear stress, provide a real time insight into the dynamic process of platelet aggregation and investigation of… (more)

Subjects/Keywords: Biochemistry.

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kaur, H. (2011). DEVELOPMENT AND VALIDATION OF A FLOW DEVICE TO STUDY PLATELET FUNCTION IN VITRO AND ELUCIDATING THE ROLE OF THYMOSIN β 4 IN VARIOUS PHYSIOLOGICAL PROCESSES. (Doctoral Dissertation). National Library of Canada. Retrieved from http://scholar.uwindsor.ca/etd/393

Chicago Manual of Style (16^th Edition):

Kaur, Harmanpreet. “DEVELOPMENT AND VALIDATION OF A FLOW DEVICE TO STUDY PLATELET FUNCTION IN VITRO AND ELUCIDATING THE ROLE OF THYMOSIN β 4 IN VARIOUS PHYSIOLOGICAL PROCESSES.” 2011. Doctoral Dissertation, National Library of Canada. Accessed February 27, 2021. http://scholar.uwindsor.ca/etd/393.

MLA Handbook (7^th Edition):

Kaur, Harmanpreet. “DEVELOPMENT AND VALIDATION OF A FLOW DEVICE TO STUDY PLATELET FUNCTION IN VITRO AND ELUCIDATING THE ROLE OF THYMOSIN β 4 IN VARIOUS PHYSIOLOGICAL PROCESSES.” 2011. Web. 27 Feb 2021.

Vancouver:

Kaur H. DEVELOPMENT AND VALIDATION OF A FLOW DEVICE TO STUDY PLATELET FUNCTION IN VITRO AND ELUCIDATING THE ROLE OF THYMOSIN β 4 IN VARIOUS PHYSIOLOGICAL PROCESSES. [Internet] [Doctoral dissertation]. National Library of Canada; 2011. [cited 2021 Feb 27]. Available from: http://scholar.uwindsor.ca/etd/393.

Council of Science Editors:

Kaur H. DEVELOPMENT AND VALIDATION OF A FLOW DEVICE TO STUDY PLATELET FUNCTION IN VITRO AND ELUCIDATING THE ROLE OF THYMOSIN β 4 IN VARIOUS PHYSIOLOGICAL PROCESSES. [Doctoral Dissertation]. National Library of Canada; 2011. Available from: http://scholar.uwindsor.ca/etd/393

8. Lek, Mark T. Design of novel chimeras provides insight into structure/function activity of apolipoprotein E3 and apolipoprotein AI.

Degree: 2017, California State University, Long Beach

URL: http://pqdtopen.proquest.com/#viewpdf?dispub=10196288

► Apolipoprotein (apo) E3 (299 residues, ∼34 kDa) and apoAI (243 residues, ∼28 kDa) are exchangeable apolipoproteins that play a dominant role in regulating plasma… (more)

Subjects/Keywords: Biochemistry

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lek, M. T. (2017). Design of novel chimeras provides insight into structure/function activity of apolipoprotein E3 and apolipoprotein AI. (Thesis). California State University, Long Beach. Retrieved from http://pqdtopen.proquest.com/#viewpdf?dispub=10196288

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lek, Mark T. “Design of novel chimeras provides insight into structure/function activity of apolipoprotein E3 and apolipoprotein AI.” 2017. Thesis, California State University, Long Beach. Accessed February 27, 2021. http://pqdtopen.proquest.com/#viewpdf?dispub=10196288.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lek, Mark T. “Design of novel chimeras provides insight into structure/function activity of apolipoprotein E3 and apolipoprotein AI.” 2017. Web. 27 Feb 2021.

Vancouver:

Lek MT. Design of novel chimeras provides insight into structure/function activity of apolipoprotein E3 and apolipoprotein AI. [Internet] [Thesis]. California State University, Long Beach; 2017. [cited 2021 Feb 27]. Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=10196288.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lek MT. Design of novel chimeras provides insight into structure/function activity of apolipoprotein E3 and apolipoprotein AI. [Thesis]. California State University, Long Beach; 2017. Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=10196288

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

9. Hauser, Kevin Eduard. Complementarity in the structure and dynamics of protein-DNA search and recognition| A multiscale modeling study.

Degree: 2017, State University of New York at Stony Brook

URL: http://pqdtopen.proquest.com/#viewpdf?dispub=10248000

► Transcription factors (TF) interact with DNA to regulate gene expression by specifically binding one or two sequences out of millions of possible sites in… (more)

▼ Transcription factors (TF) interact with DNA to regulate gene expression by specifically binding one or two sequences out of millions of possible sites in a genome. Sequence specific binding (recognition) arises from two mechanisms: amino acid-nucleobase H-bonding (direct readout), and sequence-dependent DNA deformability and structure (indirect readout). These lead to tight, specific binding. TFs can also rapidly search for their target by attaching to any sequence and then sliding along DNA. This decreases the space through which a TF randomly walks to stumble on the target. Tight binding and rapid sliding are optimized because TFs can switch between different DNA-binding modes, a search mode and a recognition mode. Due to the disorder during search and the dynamics during recognition, the atomic level dynamics are too transient to see by experimental techniques. Therefore my goal was to develop an atomistic model of protein-DNA search and recognition using computer simulations. To meet this goal I studied a model TF, the human mitochondrial transcription termination factor-1 (MTERF1), which has a modular superhelical-architecture complementary to DNA. I used molecular dynamics (MD) simulations to characterize MTERF1 search and recognition, alongside other computational methods to generate my structural models. I was the first to observe spontaneous sliding of a TF on DNA. I found that flexibility between modules permitted contacts along the DNA footprint to shift independently. This implies that the net sliding barrier of MTERF1 is similar to the barrier for one module-DNA contact rather than the sum of all module-DNA contacts. Next I utilized enhanced sampling to drive MTERF1 over the recognition barrier(s). I found that the DNA deformed as MTERF1 made increasingly more direct readout contacts to DNA, with the global recognition process characterized by partnered DNA and MTERF1 helix unwinding. These results suggest that direct and indirect readout occur simultaneously during recognition as MTERF1 unwinds (deforms) the target DNA sequence. Helix unwinding motions were present in search mode, suggesting the dynamics that drive search and recognition are intrinsic to the MTERF1 architecture.

Subjects/Keywords: Biochemistry

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hauser, K. E. (2017). Complementarity in the structure and dynamics of protein-DNA search and recognition| A multiscale modeling study. (Thesis). State University of New York at Stony Brook. Retrieved from http://pqdtopen.proquest.com/#viewpdf?dispub=10248000

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Hauser, Kevin Eduard. “Complementarity in the structure and dynamics of protein-DNA search and recognition| A multiscale modeling study.” 2017. Thesis, State University of New York at Stony Brook. Accessed February 27, 2021. http://pqdtopen.proquest.com/#viewpdf?dispub=10248000.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Hauser, Kevin Eduard. “Complementarity in the structure and dynamics of protein-DNA search and recognition| A multiscale modeling study.” 2017. Web. 27 Feb 2021.

Vancouver:

Hauser KE. Complementarity in the structure and dynamics of protein-DNA search and recognition| A multiscale modeling study. [Internet] [Thesis]. State University of New York at Stony Brook; 2017. [cited 2021 Feb 27]. Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=10248000.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hauser KE. Complementarity in the structure and dynamics of protein-DNA search and recognition| A multiscale modeling study. [Thesis]. State University of New York at Stony Brook; 2017. Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=10248000

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

The George Washington University

10. Pendo, Kathryn Margaret. Development of Delivery by Schistosomes of Broadly Neutralizing Anti-HIV-1 Antibody.

Degree: 2017, The George Washington University

URL: http://pqdtopen.proquest.com/#viewpdf?dispub=10272646

► Introduction. The overarching goal of this study is to determine whether a long-lived, transgenic helminth parasite might express a potentially therapeutic antibody, using HIV-1… (more)

Subjects/Keywords: Biochemistry

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Pendo, K. M. (2017). Development of Delivery by Schistosomes of Broadly Neutralizing Anti-HIV-1 Antibody. (Thesis). The George Washington University. Retrieved from http://pqdtopen.proquest.com/#viewpdf?dispub=10272646

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Pendo, Kathryn Margaret. “Development of Delivery by Schistosomes of Broadly Neutralizing Anti-HIV-1 Antibody.” 2017. Thesis, The George Washington University. Accessed February 27, 2021. http://pqdtopen.proquest.com/#viewpdf?dispub=10272646.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Pendo, Kathryn Margaret. “Development of Delivery by Schistosomes of Broadly Neutralizing Anti-HIV-1 Antibody.” 2017. Web. 27 Feb 2021.

Vancouver:

Pendo KM. Development of Delivery by Schistosomes of Broadly Neutralizing Anti-HIV-1 Antibody. [Internet] [Thesis]. The George Washington University; 2017. [cited 2021 Feb 27]. Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=10272646.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Pendo KM. Development of Delivery by Schistosomes of Broadly Neutralizing Anti-HIV-1 Antibody. [Thesis]. The George Washington University; 2017. Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=10272646

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of California – Berkeley

11. Dietrich, Jeffrey Allen. Transcription Factor-Based Small-Molecule Screens and Selections.

Degree: Bioengineering, 2011, University of California – Berkeley

URL: http://www.escholarship.org/uc/item/7j6805gf

► Directed evolution of E. coli for improved small-molecule production requires a combination of rational design and high-throughput screening technologies. Rational design-based directed evolution schemes use… (more)

▼ Directed evolution of E. coli for improved small-molecule production requires a combination of rational design and high-throughput screening technologies. Rational design-based directed evolution schemes use structural analyses and metabolic models to help identify targets for mutagenesis, thus improving the likelihood of identifying the desired phenotype. We used a strictly rational design-based approach to re-engineer cytochrome P450_BM3 for epoxidation of amorphadiene, developing a novel route for production of the anti-malarial compound artemisinin. A model structure of the lowest energy transition state complex for amorphadiene in the P450_BM3 active site was created using ROSETTA-based energy minimization. The resulting enzyme variant produced artemisinic-11S,12-epoxide at titers greater than 250 mg*l^-1. Continued attempts to use ROSETTA and to either improve P450_BM3 epoxidase activity or introduce hydroxylase activity, however, proved unsuccessful. In the absence of a high-throughput screening approach, further improvement of the P450-based production system would be difficult.As with most small-molecules, there exists no known high-throughput screen for artemisinic-11S-12-epoxide, amorphadiene, or any structurally-related compound. We hypothesized that a generalized method for high-throughput screen or selection design could be based on transcription factor-promoter pairs responding to the target small-molecule. Transcription factors have long been used to construct whole-cell biosensors for the detection of environmental small-molecule pollutants¹, but the work has remained largely un-translated toward screen development. While no known transcription factor binds artemisinic epoxide, a putative transcription factor-promoter pair responsive to 1-butanol, a biofuel molecule of interest in our laboratory, was recently reported². The transcription factor, BmoR, and its cognate promoter, P_BMO, were used to build a short-chain alcohol biosensor for use as a genetic screen or selection. Following optimization of expression temperature, promoter, and reporter 5'-untranslated region, among other parameters, the BmoR-P_BMO system was shown to provide robust detection of 1-butanol in an E. coli host. The biosensor transfer function - relating input alcohol concentration to output fluorescent signal - was derived for 1-butanol and structurally related alcohols using the Hill Equation. The biosensor exhibited a linear response between 100 µM and 40 mM 1-butanol, and a dynamic range of over 8000 GFP/OD600 units. A 700 µM difference in 1-butanol concentration could be detected at 95% confidence. By replacing the GFP reporter with TetA, a tetracycline transporter, a 1-butanol selection was constructed; E. coli harboring the TetA-based biosensor exhibited 1-butanol dependent growth in the presence of tetracycline up to 40 mM exogenously added…

Subjects/Keywords: Biochemistry

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Dietrich, J. A. (2011). Transcription Factor-Based Small-Molecule Screens and Selections. (Thesis). University of California – Berkeley. Retrieved from http://www.escholarship.org/uc/item/7j6805gf

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Dietrich, Jeffrey Allen. “Transcription Factor-Based Small-Molecule Screens and Selections.” 2011. Thesis, University of California – Berkeley. Accessed February 27, 2021. http://www.escholarship.org/uc/item/7j6805gf.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Dietrich, Jeffrey Allen. “Transcription Factor-Based Small-Molecule Screens and Selections.” 2011. Web. 27 Feb 2021.

Vancouver:

Dietrich JA. Transcription Factor-Based Small-Molecule Screens and Selections. [Internet] [Thesis]. University of California – Berkeley; 2011. [cited 2021 Feb 27]. Available from: http://www.escholarship.org/uc/item/7j6805gf.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Dietrich JA. Transcription Factor-Based Small-Molecule Screens and Selections. [Thesis]. University of California – Berkeley; 2011. Available from: http://www.escholarship.org/uc/item/7j6805gf

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of California – Berkeley

12. Wong, Roger Hoi Fung. Regulation of lipogenic gene transcription during fasting and feeding/insulin: Role of USF, SREBP-1c, and BAF60c.

Degree: Comparative Biochemistry, 2010, University of California – Berkeley

URL: http://www.escholarship.org/uc/item/3zc4j28d

► Transcription of genes encoding enzymes involved in fatty acid and triacylglycerol synthesis, including fatty acid synthase and mitochondrial glycerol-3-phosphate acyltransferase, is coordinately induced in lipogenic… (more)

▼ Transcription of genes encoding enzymes involved in fatty acid and triacylglycerol synthesis, including fatty acid synthase and mitochondrial glycerol-3-phosphate acyltransferase, is coordinately induced in lipogenic tissues by feeding and insulin treatment. Dysregulation of lipognesis often contributes to metabolic diseases such as obesity, diabetes, and cardiovascular diseases. Transcription factors and signaling molecules involved in transcriptional activation of lipogenesis represent attractive targets for the prevention and treatment of metabolic diseases. In transcriptional activation of fatty acid synthase by feeding/insulin, USF constitutively bound to the -65 E-box is required. In this study, USF was shown to function as a molecular switch by recruiting various interacting proteins during the fasting/feeding transition. First, USF was detected to directly interact with SREBP-1 that is induced by feeding and binds nearby -150 SRE. Cotransfection of USF and SREBP-1c with an FAS promoter-luciferase reporter construct resulted in high synergistic activation of the FAS promoter. Chromatin immunoprecipitation analysis of mouse liver demonstrated that USF binds constitutively to the fatty acid synthase promoter during fasting/feeding, whereas the binding of SREBP-1 was observed only during feeding, in a manner identical to that of the FAS promoter. These data show that USF recruits SREBP-1c to the lipogenic gene promoters upon feeding/insulin. Similar cooperative action of USF and SREBP-1c in the activation of the mitochondrial glycerol-3-phosphate acyltransferase promoter was also observed.During feeding/insulin, USF-1 recruits three distinct families of proteins to the lipogenic promoters: 1) P/CAF which acetylates USF and functions as a coactivator, 2) DNA break/repair machinery including Ku70, Ku80, PARP-1, TopoIIB and DNA-PK, which causes a DNA break in the lipogenic gene promoter prior to the transcriptional initiation in vivo, and 3) signaling molecules including PP1 and DNA-PK. PP1 dephosphorylates and activates DNA-PK upon feeding/insulin treatment. Thus in the fed state, activated DNA-PK phosphorylates USF-1 at S262, allowing the recruitment of and acetylation by P/CAF at K237, leading to promoter activation. P/CAF-mediated acetylation of USF is reversed by HDAC9 in the fasted state. Although total HDAC9 levels do not change during fasting/feeding, nuclear abundance of HDAC9 increases upon fasting, suggesting regulation of HDAC9 by nuclear translocation. DNA break/repair components associated with USF also bring about transient DNA breaks during feeding-induced FAS activation. In DNA-PK deficient SCID mice, feeding induced USF-1 phosphorylation/acetylation, DNA-breaks, and FAS activation leading to lipogenesis are impaired, resulting in decreased liver and circulating triglyceride levels. This study demonstrates that DNA-PK mediates the feeding/insulin-dependent lipogenic gene activation. BAF60c was also detected to be an USF interacting protein recruited to lipogenic gene promoters upon feeding. Among…

Subjects/Keywords: Biochemistry

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wong, R. H. F. (2010). Regulation of lipogenic gene transcription during fasting and feeding/insulin: Role of USF, SREBP-1c, and BAF60c. (Thesis). University of California – Berkeley. Retrieved from http://www.escholarship.org/uc/item/3zc4j28d

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Wong, Roger Hoi Fung. “Regulation of lipogenic gene transcription during fasting and feeding/insulin: Role of USF, SREBP-1c, and BAF60c.” 2010. Thesis, University of California – Berkeley. Accessed February 27, 2021. http://www.escholarship.org/uc/item/3zc4j28d.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Wong, Roger Hoi Fung. “Regulation of lipogenic gene transcription during fasting and feeding/insulin: Role of USF, SREBP-1c, and BAF60c.” 2010. Web. 27 Feb 2021.

Vancouver:

Wong RHF. Regulation of lipogenic gene transcription during fasting and feeding/insulin: Role of USF, SREBP-1c, and BAF60c. [Internet] [Thesis]. University of California – Berkeley; 2010. [cited 2021 Feb 27]. Available from: http://www.escholarship.org/uc/item/3zc4j28d.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Wong RHF. Regulation of lipogenic gene transcription during fasting and feeding/insulin: Role of USF, SREBP-1c, and BAF60c. [Thesis]. University of California – Berkeley; 2010. Available from: http://www.escholarship.org/uc/item/3zc4j28d

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of California – San Diego

13. Du, Qian. Bcl3 and REG-gamma are the Regulators of NF-kappaB p50 and p52.

Degree: Chemistry, 2017, University of California – San Diego

URL: http://www.escholarship.org/uc/item/2293k9sk

► Apart from the canonical and non-canonical activation pathway of NF-κB, the atypical IκB family member Bcl3, can regulate p50 and p52 homodimers’ activity. These dimers,… (more)

Subjects/Keywords: Biochemistry

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Du, Q. (2017). Bcl3 and REG-gamma are the Regulators of NF-kappaB p50 and p52. (Thesis). University of California – San Diego. Retrieved from http://www.escholarship.org/uc/item/2293k9sk

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Du, Qian. “Bcl3 and REG-gamma are the Regulators of NF-kappaB p50 and p52.” 2017. Thesis, University of California – San Diego. Accessed February 27, 2021. http://www.escholarship.org/uc/item/2293k9sk.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Du, Qian. “Bcl3 and REG-gamma are the Regulators of NF-kappaB p50 and p52.” 2017. Web. 27 Feb 2021.

Vancouver:

Du Q. Bcl3 and REG-gamma are the Regulators of NF-kappaB p50 and p52. [Internet] [Thesis]. University of California – San Diego; 2017. [cited 2021 Feb 27]. Available from: http://www.escholarship.org/uc/item/2293k9sk.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Du Q. Bcl3 and REG-gamma are the Regulators of NF-kappaB p50 and p52. [Thesis]. University of California – San Diego; 2017. Available from: http://www.escholarship.org/uc/item/2293k9sk

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of California – San Diego

14. Pu, Yuan. UTILIZING CHLAMYDOMONAS REINHARDTII AS A PLATFORM TO STUDY METABOLIC ENGINEERING.

Degree: Chemistry, 2016, University of California – San Diego

URL: http://www.escholarship.org/uc/item/1zh1641q

► Paclitaxel, as known as taxol, is a member of a large family terpenoid and it is famous for its anti-cancer properties. It was originally isolated… (more)

Subjects/Keywords: Biochemistry

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Pu, Y. (2016). UTILIZING CHLAMYDOMONAS REINHARDTII AS A PLATFORM TO STUDY METABOLIC ENGINEERING. (Thesis). University of California – San Diego. Retrieved from http://www.escholarship.org/uc/item/1zh1641q

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Pu, Yuan. “UTILIZING CHLAMYDOMONAS REINHARDTII AS A PLATFORM TO STUDY METABOLIC ENGINEERING.” 2016. Thesis, University of California – San Diego. Accessed February 27, 2021. http://www.escholarship.org/uc/item/1zh1641q.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Pu, Yuan. “UTILIZING CHLAMYDOMONAS REINHARDTII AS A PLATFORM TO STUDY METABOLIC ENGINEERING.” 2016. Web. 27 Feb 2021.

Vancouver:

Pu Y. UTILIZING CHLAMYDOMONAS REINHARDTII AS A PLATFORM TO STUDY METABOLIC ENGINEERING. [Internet] [Thesis]. University of California – San Diego; 2016. [cited 2021 Feb 27]. Available from: http://www.escholarship.org/uc/item/1zh1641q.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Pu Y. UTILIZING CHLAMYDOMONAS REINHARDTII AS A PLATFORM TO STUDY METABOLIC ENGINEERING. [Thesis]. University of California – San Diego; 2016. Available from: http://www.escholarship.org/uc/item/1zh1641q

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of California – San Diego

15. Ko, Juyeon. BioID Utilized to Identify Proteins Mediating Signaling Crosstalk between IKKbeta and STAT3.

Degree: Chemistry, 2016, University of California – San Diego

URL: http://www.escholarship.org/uc/item/2053146x

► IKKβ, which is the main protein kinase in the canonical NF-κB signaling, regulates cytokine production, inflammation, cell proliferation, survival and the responses to cellular stress.… (more)

Subjects/Keywords: Biochemistry

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ko, J. (2016). BioID Utilized to Identify Proteins Mediating Signaling Crosstalk between IKKbeta and STAT3. (Thesis). University of California – San Diego. Retrieved from http://www.escholarship.org/uc/item/2053146x

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ko, Juyeon. “BioID Utilized to Identify Proteins Mediating Signaling Crosstalk between IKKbeta and STAT3.” 2016. Thesis, University of California – San Diego. Accessed February 27, 2021. http://www.escholarship.org/uc/item/2053146x.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ko, Juyeon. “BioID Utilized to Identify Proteins Mediating Signaling Crosstalk between IKKbeta and STAT3.” 2016. Web. 27 Feb 2021.

Vancouver:

Ko J. BioID Utilized to Identify Proteins Mediating Signaling Crosstalk between IKKbeta and STAT3. [Internet] [Thesis]. University of California – San Diego; 2016. [cited 2021 Feb 27]. Available from: http://www.escholarship.org/uc/item/2053146x.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ko J. BioID Utilized to Identify Proteins Mediating Signaling Crosstalk between IKKbeta and STAT3. [Thesis]. University of California – San Diego; 2016. Available from: http://www.escholarship.org/uc/item/2053146x

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

16. Mednick, Gabriel Franchesco. Structural And Functional Characterization Of A Bacterial Photosensing Light-Oxygen-Voltage (LOV) Protein Domain From Rhizobium Leguminosarum.

Degree: Chemistry, 2016, University of California – Santa Cruz

URL: http://www.escholarship.org/uc/item/23b297c7

► Light-Oxygen-Voltage (LOV) domains are an important family of sensors that can be found in bacteria, archaea and eukaryotes. This thesis focuses on the blue light-sensing… (more)

▼ Light-Oxygen-Voltage (LOV) domains are an important family of sensors that can be found in bacteria, archaea and eukaryotes. This thesis focuses on the blue light-sensing LOV domains and their signal transduction pathways (STPs) in bacteria. Specifically, a LOV domain from Rhizobium leguminosarum (Rle) is characterized. Biophysical characterization of the protein’s cyclic light response (photocycle) and structure were addressed in order to further our understanding of this important signalling pathway. Chapter 1 introduces the fundamental characteristics of light sensing with a specific emphasis on bacterial LOV domains. After introducing these sensors and their associated signalling pathways, Chapter 2 focuses on a LOV-histidine ki- nase (HK) from Rle. The importance of Rhizobium-plant symbiosis and previous research on the in vivo characterization of the Rh-LOV-HK STP are summarized. Then, the cloning, protein expression and purification methods that were used throughout this thesis research are described. In the results section, various optical spectroscopy experiments that were used to characterize the photocycle properties of Rh-LOV-HK and the Rh-LOV domain in isolation are presented. Experiments included time-resolved flow-flash absorption measurements to measure early changes in the photocycle, and absorption and fluorescence spectroscopy to characterize the average light-activated (adduct) state lifetime. Chapter 2 con- cludes with circular dichroism (CD) experiments, which can provide insights into the conformational changes that lead to the active signalling state. In Chapter 3, the experimental procedures that were used to crystallize and solve the structure of Rh-LOV at 1.89 A resolution are described. Analysis of this high-resolution structure includes a close look at interactions with FMN in the active pocket, the dimerization interface and the position of the C-terminal region. For comparison, the structure of Rh-LOV is contrasted with previously crystallized bacterial LOV domain structures. These homologous LOV domains can have distinctly different temporal photocycle characteristics, which may arise from small differences in structure. Finally, we discuss the experimental procedures that were carried out with the intention of resolving the light-activated crystal structure of Rh-LOV. In Chapter 4, the structural-functional characterization of Rh-LOV using electron paramagnetic resonance (EPR) techniques is presented. At various positions in the Rh-LOV structure, we show that changes in the continuous wave (CW) EPR spectrum of a protein-bound paramagnetic label corresponds to the localized protein environment. Using the pulsed EPR technique, double electron-electron resonance (DEER), distance probability distributions were determined between various positions in the Rh-LOV solution structure. We show that it is a dimer and compare the solution and crystal structure dimer orientations. The results from DEER experiments that were undertaken to…

Subjects/Keywords: Biochemistry

2 more images…

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mednick, G. F. (2016). Structural And Functional Characterization Of A Bacterial Photosensing Light-Oxygen-Voltage (LOV) Protein Domain From Rhizobium Leguminosarum. (Thesis). University of California – Santa Cruz. Retrieved from http://www.escholarship.org/uc/item/23b297c7

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mednick, Gabriel Franchesco. “Structural And Functional Characterization Of A Bacterial Photosensing Light-Oxygen-Voltage (LOV) Protein Domain From Rhizobium Leguminosarum.” 2016. Thesis, University of California – Santa Cruz. Accessed February 27, 2021. http://www.escholarship.org/uc/item/23b297c7.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mednick, Gabriel Franchesco. “Structural And Functional Characterization Of A Bacterial Photosensing Light-Oxygen-Voltage (LOV) Protein Domain From Rhizobium Leguminosarum.” 2016. Web. 27 Feb 2021.

Vancouver:

Mednick GF. Structural And Functional Characterization Of A Bacterial Photosensing Light-Oxygen-Voltage (LOV) Protein Domain From Rhizobium Leguminosarum. [Internet] [Thesis]. University of California – Santa Cruz; 2016. [cited 2021 Feb 27]. Available from: http://www.escholarship.org/uc/item/23b297c7.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mednick GF. Structural And Functional Characterization Of A Bacterial Photosensing Light-Oxygen-Voltage (LOV) Protein Domain From Rhizobium Leguminosarum. [Thesis]. University of California – Santa Cruz; 2016. Available from: http://www.escholarship.org/uc/item/23b297c7

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of California – San Diego

17. Tran, Eileen Thuc Duyen. Characterizing a Unique IRES in Kaposi's Sarcoma-Associated Herpesvirus.

Degree: Chemistry, 2016, University of California – San Diego

URL: http://www.escholarship.org/uc/item/5n8935d1

► The synthesis of proteins during eukaryotic translation involves four major phases, where initiation is often the rate-limiting step and target for translational control with a… (more)

Subjects/Keywords: Biochemistry

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Tran, E. T. D. (2016). Characterizing a Unique IRES in Kaposi's Sarcoma-Associated Herpesvirus. (Thesis). University of California – San Diego. Retrieved from http://www.escholarship.org/uc/item/5n8935d1

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Tran, Eileen Thuc Duyen. “Characterizing a Unique IRES in Kaposi's Sarcoma-Associated Herpesvirus.” 2016. Thesis, University of California – San Diego. Accessed February 27, 2021. http://www.escholarship.org/uc/item/5n8935d1.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Tran, Eileen Thuc Duyen. “Characterizing a Unique IRES in Kaposi's Sarcoma-Associated Herpesvirus.” 2016. Web. 27 Feb 2021.

Vancouver:

Tran ETD. Characterizing a Unique IRES in Kaposi's Sarcoma-Associated Herpesvirus. [Internet] [Thesis]. University of California – San Diego; 2016. [cited 2021 Feb 27]. Available from: http://www.escholarship.org/uc/item/5n8935d1.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Tran ETD. Characterizing a Unique IRES in Kaposi's Sarcoma-Associated Herpesvirus. [Thesis]. University of California – San Diego; 2016. Available from: http://www.escholarship.org/uc/item/5n8935d1

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of California – Berkeley

18. Hejab, Nisreen M A. Characterization of Tau and Anticancer Drug Interactions with Microtubules via Cryo-Electron Microscopy Studies.

Degree: Comparative Biochemistry, 2016, University of California – Berkeley

URL: http://www.escholarship.org/uc/item/7zb0x85p

► Microtubules (MTs) are essential components of the eukaryotic cytoskeleton. They form by the polymerization of tubulin into cylindrical polymers composed of protofilaments. MTs are involved… (more)

▼ Microtubules (MTs) are essential components of the eukaryotic cytoskeleton. They form by the polymerization of tubulin into cylindrical polymers composed of protofilaments. MTs are involved in a diverse array of cellular functions due to their dynamic instability, which is modulated by many factors in vivo and in vitro. Agents that modulate MT dynamics include MT associated proteins (MAPs) and naturally occurring compounds known as MT stabilizing agents (MSAs). A number of MSAs have demonstrated or predicted potential as anticancer agents, but a detailed structural basis for their mechanism of action is still lacking. We have used cryo-electron microscopy (cryo-EM) to study the structural basis of action of zampanolide, a taxane-binding site (TBS) MSA, as well as several proteins known to stabilize MTs, with main focus on tau. We have obtained a high-resolution (4.2 Å) cryo-EM reconstruction of microtubules stabilized by zampanolide and have compared it to Taxol, which binds to the same pocket on β-tubulin. We find that each TBS MSA has distinct structural effects on the microtubule (MT) lattice. Binding of Taxol or zampanolide both induce MT heterogeneity, but each affects the longitudinal interface differently.Tau is the prominent neuronal MAP and it has been implicated with Alzheimer disease (AD). Tau is known to bind and stabilize MTs in the axons but its binding mode and stabilizing mechanism are not well understood. Our study shows that tau binds exclusively to the external surface of MTs along protofilaments and that it remains in an extended confirmation. In addition, in the presence of kinesin, full length tau (FL-tau) dissociates from MTs allowing kinesin binding. Surprisingly, sub-stoichiometric amounts of tau result in the formation of GDP-tubulin double rings that disappear upon increasing the amounts of tau added to MTs. FL-tau and minimal 2-R or 4-R tau constructs result in the formation of sleeve-like structures around MTs, suggesting that tau binds to tubulin dimers as well as to fully polymerized MTs. Thus, we hypothesize that tau functions by oligomerizing tubulin dimers leading to a compacted MT lattice that could reinforce the interactions across the longitudinal MT interface. Furthermore, we identified amino acids on the surface of tubulin that could interact with tau. Tau binds to MTs near the C-terminus, and the sequestering of the acidic C-terminus of tubulin could contribute to the stability of MTs in the presence of tau.

Subjects/Keywords: Biochemistry

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hejab, N. M. A. (2016). Characterization of Tau and Anticancer Drug Interactions with Microtubules via Cryo-Electron Microscopy Studies. (Thesis). University of California – Berkeley. Retrieved from http://www.escholarship.org/uc/item/7zb0x85p

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Hejab, Nisreen M A. “Characterization of Tau and Anticancer Drug Interactions with Microtubules via Cryo-Electron Microscopy Studies.” 2016. Thesis, University of California – Berkeley. Accessed February 27, 2021. http://www.escholarship.org/uc/item/7zb0x85p.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Hejab, Nisreen M A. “Characterization of Tau and Anticancer Drug Interactions with Microtubules via Cryo-Electron Microscopy Studies.” 2016. Web. 27 Feb 2021.

Vancouver:

Hejab NMA. Characterization of Tau and Anticancer Drug Interactions with Microtubules via Cryo-Electron Microscopy Studies. [Internet] [Thesis]. University of California – Berkeley; 2016. [cited 2021 Feb 27]. Available from: http://www.escholarship.org/uc/item/7zb0x85p.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hejab NMA. Characterization of Tau and Anticancer Drug Interactions with Microtubules via Cryo-Electron Microscopy Studies. [Thesis]. University of California – Berkeley; 2016. Available from: http://www.escholarship.org/uc/item/7zb0x85p

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of California – Merced

19. Nguyen, Loan Thi Cam. DELIVERY OF ANTI-INFLAMMATORY PROTEINS ACROSS THE HUMAN BLOOD BRAIN BARRIER FOR THE TREATMENT OF TRAUMATIC BRAIN INJURY AND RELATED PATHOLOGIES.

Degree: Chemistry and Chemical Biology, 2016, University of California – Merced

URL: http://www.escholarship.org/uc/item/86g4b3cn

► Traumatic Brain Injury (TBI) is an injury that occurs following sudden head trauma and results in extensive damage to brain tissue primarily due to the… (more)

Subjects/Keywords: Biochemistry

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Nguyen, L. T. C. (2016). DELIVERY OF ANTI-INFLAMMATORY PROTEINS ACROSS THE HUMAN BLOOD BRAIN BARRIER FOR THE TREATMENT OF TRAUMATIC BRAIN INJURY AND RELATED PATHOLOGIES. (Thesis). University of California – Merced. Retrieved from http://www.escholarship.org/uc/item/86g4b3cn

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Nguyen, Loan Thi Cam. “DELIVERY OF ANTI-INFLAMMATORY PROTEINS ACROSS THE HUMAN BLOOD BRAIN BARRIER FOR THE TREATMENT OF TRAUMATIC BRAIN INJURY AND RELATED PATHOLOGIES.” 2016. Thesis, University of California – Merced. Accessed February 27, 2021. http://www.escholarship.org/uc/item/86g4b3cn.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Nguyen, Loan Thi Cam. “DELIVERY OF ANTI-INFLAMMATORY PROTEINS ACROSS THE HUMAN BLOOD BRAIN BARRIER FOR THE TREATMENT OF TRAUMATIC BRAIN INJURY AND RELATED PATHOLOGIES.” 2016. Web. 27 Feb 2021.

Vancouver:

Nguyen LTC. DELIVERY OF ANTI-INFLAMMATORY PROTEINS ACROSS THE HUMAN BLOOD BRAIN BARRIER FOR THE TREATMENT OF TRAUMATIC BRAIN INJURY AND RELATED PATHOLOGIES. [Internet] [Thesis]. University of California – Merced; 2016. [cited 2021 Feb 27]. Available from: http://www.escholarship.org/uc/item/86g4b3cn.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Nguyen LTC. DELIVERY OF ANTI-INFLAMMATORY PROTEINS ACROSS THE HUMAN BLOOD BRAIN BARRIER FOR THE TREATMENT OF TRAUMATIC BRAIN INJURY AND RELATED PATHOLOGIES. [Thesis]. University of California – Merced; 2016. Available from: http://www.escholarship.org/uc/item/86g4b3cn

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of California – Santa Cruz

20. Liban, Tyler James. Structural Conservation and E2F Binding Specificity Within the Retinoblastoma Pocket Protein Family.

Degree: Chemistry, 2016, University of California – Santa Cruz

URL: http://www.escholarship.org/uc/item/9wc5k495

► The regulation of cell division is critical for ensuring the outcome of two viable daughter cells. Improper regulation of the cell cycle can lead to… (more)

Subjects/Keywords: Biochemistry

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Liban, T. J. (2016). Structural Conservation and E2F Binding Specificity Within the Retinoblastoma Pocket Protein Family. (Thesis). University of California – Santa Cruz. Retrieved from http://www.escholarship.org/uc/item/9wc5k495

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Liban, Tyler James. “Structural Conservation and E2F Binding Specificity Within the Retinoblastoma Pocket Protein Family.” 2016. Thesis, University of California – Santa Cruz. Accessed February 27, 2021. http://www.escholarship.org/uc/item/9wc5k495.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Liban, Tyler James. “Structural Conservation and E2F Binding Specificity Within the Retinoblastoma Pocket Protein Family.” 2016. Web. 27 Feb 2021.

Vancouver:

Liban TJ. Structural Conservation and E2F Binding Specificity Within the Retinoblastoma Pocket Protein Family. [Internet] [Thesis]. University of California – Santa Cruz; 2016. [cited 2021 Feb 27]. Available from: http://www.escholarship.org/uc/item/9wc5k495.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Liban TJ. Structural Conservation and E2F Binding Specificity Within the Retinoblastoma Pocket Protein Family. [Thesis]. University of California – Santa Cruz; 2016. Available from: http://www.escholarship.org/uc/item/9wc5k495

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of California – San Diego

21. Huang, Andrea. Lipases of Branched Fatty Acid Esters of Hydroxy Fatty Acids in Drosophila melanogaster.

Degree: Biology, 2017, University of California – San Diego

URL: http://www.escholarship.org/uc/item/0sr9n8pf

► A recently discovered class of endogenous lipids called fatty acid esters of hydroxy fatty acids (FAHFAs) display potent anti-diabetic and anti-inflammatory effects. This leads to… (more)

Subjects/Keywords: Biochemistry

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Huang, A. (2017). Lipases of Branched Fatty Acid Esters of Hydroxy Fatty Acids in Drosophila melanogaster. (Thesis). University of California – San Diego. Retrieved from http://www.escholarship.org/uc/item/0sr9n8pf

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Huang, Andrea. “Lipases of Branched Fatty Acid Esters of Hydroxy Fatty Acids in Drosophila melanogaster.” 2017. Thesis, University of California – San Diego. Accessed February 27, 2021. http://www.escholarship.org/uc/item/0sr9n8pf.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Huang, Andrea. “Lipases of Branched Fatty Acid Esters of Hydroxy Fatty Acids in Drosophila melanogaster.” 2017. Web. 27 Feb 2021.

Vancouver:

Huang A. Lipases of Branched Fatty Acid Esters of Hydroxy Fatty Acids in Drosophila melanogaster. [Internet] [Thesis]. University of California – San Diego; 2017. [cited 2021 Feb 27]. Available from: http://www.escholarship.org/uc/item/0sr9n8pf.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Huang A. Lipases of Branched Fatty Acid Esters of Hydroxy Fatty Acids in Drosophila melanogaster. [Thesis]. University of California – San Diego; 2017. Available from: http://www.escholarship.org/uc/item/0sr9n8pf

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of California – San Diego

22. Zhang, Yue. Loss of Usp18 Impairs the Propagation of Myeloid Leukemia Cells in Mouse Models.

Degree: Biology, 2017, University of California – San Diego

URL: http://www.escholarship.org/uc/item/16f0p00k

► Acute Myloid Leukemia (AML) is one of the most common leukemia in adults with poor five years survival even with modern chemotherapy treatment. New strategies… (more)

Subjects/Keywords: Biochemistry

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Zhang, Y. (2017). Loss of Usp18 Impairs the Propagation of Myeloid Leukemia Cells in Mouse Models. (Thesis). University of California – San Diego. Retrieved from http://www.escholarship.org/uc/item/16f0p00k

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Zhang, Yue. “Loss of Usp18 Impairs the Propagation of Myeloid Leukemia Cells in Mouse Models.” 2017. Thesis, University of California – San Diego. Accessed February 27, 2021. http://www.escholarship.org/uc/item/16f0p00k.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Zhang, Yue. “Loss of Usp18 Impairs the Propagation of Myeloid Leukemia Cells in Mouse Models.” 2017. Web. 27 Feb 2021.

Vancouver:

Zhang Y. Loss of Usp18 Impairs the Propagation of Myeloid Leukemia Cells in Mouse Models. [Internet] [Thesis]. University of California – San Diego; 2017. [cited 2021 Feb 27]. Available from: http://www.escholarship.org/uc/item/16f0p00k.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Zhang Y. Loss of Usp18 Impairs the Propagation of Myeloid Leukemia Cells in Mouse Models. [Thesis]. University of California – San Diego; 2017. Available from: http://www.escholarship.org/uc/item/16f0p00k

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of California – San Diego

23. Chang, Kimberly. Biochemical investigation of Amm3, an unusual flavin-dependent tryptophan halogenase involved in the cryptic biosynthesis of the ammosamides.

Degree: Marine biology, 2017, University of California – San Diego

URL: http://www.escholarship.org/uc/item/66d6j4r5

► Pyrroloquinoline alkaloids have been extensively isolated in the past three decades due to their broad-spectrum bioactivities. Although many total synthesis reports have described the unique… (more)

Subjects/Keywords: Biochemistry

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Chang, K. (2017). Biochemical investigation of Amm3, an unusual flavin-dependent tryptophan halogenase involved in the cryptic biosynthesis of the ammosamides. (Thesis). University of California – San Diego. Retrieved from http://www.escholarship.org/uc/item/66d6j4r5

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Chang, Kimberly. “Biochemical investigation of Amm3, an unusual flavin-dependent tryptophan halogenase involved in the cryptic biosynthesis of the ammosamides.” 2017. Thesis, University of California – San Diego. Accessed February 27, 2021. http://www.escholarship.org/uc/item/66d6j4r5.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Chang, Kimberly. “Biochemical investigation of Amm3, an unusual flavin-dependent tryptophan halogenase involved in the cryptic biosynthesis of the ammosamides.” 2017. Web. 27 Feb 2021.

Vancouver:

Chang K. Biochemical investigation of Amm3, an unusual flavin-dependent tryptophan halogenase involved in the cryptic biosynthesis of the ammosamides. [Internet] [Thesis]. University of California – San Diego; 2017. [cited 2021 Feb 27]. Available from: http://www.escholarship.org/uc/item/66d6j4r5.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Chang K. Biochemical investigation of Amm3, an unusual flavin-dependent tryptophan halogenase involved in the cryptic biosynthesis of the ammosamides. [Thesis]. University of California – San Diego; 2017. Available from: http://www.escholarship.org/uc/item/66d6j4r5

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of California – San Diego

24. Michaud, Jennifer M. The influence of microorganisms on the atmosphere and tools for the study of transacylase domains.

Degree: Chemistry, 2017, University of California – San Diego

URL: http://www.escholarship.org/uc/item/8mr2g6vv

► Specific biochemical influences on sea spray aerosol (SSA) are not well characterized and are vital to understanding the complex and variable chemical composition and atmospheric… (more)

▼ Specific biochemical influences on sea spray aerosol (SSA) are not well characterized and are vital to understanding the complex and variable chemical composition and atmospheric properties of SSA. Several studies reported in this thesis explore how microorganisms affect SSA both by examining their metabolic products in seawater and how they transfer from the seawater to aerosol, as well as the impacts of airborne microbes on SSA properties. To investigate the influence of microbial metabolism on aerosol composition, direct measurements of aerosols revealed a correlation between marine bacteria in seawater and the production of gas-phase even-chain (C:2, C:4) alkyl nitrates (RONO2). Alkyl nitrates are a large contributor to the production of tropospheric ozone and prior to these studies generally thought to originate from anthropogenic sources and produced through uv-dependent mechanisms. The results show that bacterial metabolism can contribute to the production of climate warming ozone and demonstrate an important biochemical influence on atmospheric processes. In separate studies, we explored the role of enzymatic processing of lipid metabolites by lipase in seawater and the resulting changes to aerosol morphology and composition. We observed that lipase driven digestion of lipids in seawater vastly changes the composition of SSA in simple triacylglycerol and diatom lysate systems, identifying a possible mechanism for organismal control of aerosolization.The structural organization within SSA, taxon-specificity of air-sea transfer, and ice nucleation (IN) characteristics of whole bacteria, viruses, and vesicles was explored in several studies. One study elucidated the detailed composition of seawater (bulk), SSML, and SSA using cryogenic transmission electron microscopy. The unique technique allowed the preservation and detailed examination of soft biological particles, facilitating observations of complex structures of diatoms, bacteria, viruses, and vesicles, their hydration state, and their orientation within particles. SSML derived vesicles had a more multi-lamellar structure in contrast to bulk and aerosol samples. This was the first demonstration of vesicles within SSA, indicating a possible role of these structures in climate processes. Another study examined bacterial and viral transfer across the air-sea interface using metagenomics, revealing marine bacteria aerosolize more efficiently than viruses and taxon-specific properties governing selective transfer of viruses and bacteria delineated by class and order. Several species of bacteria and viruses were consistently enriched in aerosols, and hydrophobic cell- and virus- surfaces positively influenced aerosolization. The results provide a genomic framework to elucidate aerosolization mechanisms and like the lipase studies, identify additional microbial controls of SSA composition and properties. Lastly, we examined the IN properties of halotolerant bacteria and fungi isolated from rain and SSA samples collected from Scripps Pier in La Jolla, CA. Most…

Subjects/Keywords: Biochemistry

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Michaud, J. M. (2017). The influence of microorganisms on the atmosphere and tools for the study of transacylase domains. (Thesis). University of California – San Diego. Retrieved from http://www.escholarship.org/uc/item/8mr2g6vv

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Michaud, Jennifer M. “The influence of microorganisms on the atmosphere and tools for the study of transacylase domains.” 2017. Thesis, University of California – San Diego. Accessed February 27, 2021. http://www.escholarship.org/uc/item/8mr2g6vv.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Michaud, Jennifer M. “The influence of microorganisms on the atmosphere and tools for the study of transacylase domains.” 2017. Web. 27 Feb 2021.

Vancouver:

Michaud JM. The influence of microorganisms on the atmosphere and tools for the study of transacylase domains. [Internet] [Thesis]. University of California – San Diego; 2017. [cited 2021 Feb 27]. Available from: http://www.escholarship.org/uc/item/8mr2g6vv.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Michaud JM. The influence of microorganisms on the atmosphere and tools for the study of transacylase domains. [Thesis]. University of California – San Diego; 2017. Available from: http://www.escholarship.org/uc/item/8mr2g6vv

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of California – Merced

25. Chen, Shuo. Adding new chemistry to proteins via genetic incorporation.

Degree: Chemistry and Chemical Biology, 2014, University of California – Merced

URL: http://www.escholarship.org/uc/item/9jb4g6jb

► ABSTRACTAdding New Chemistry to Proteins via Genetic IncorporationbyShuo Chen, B.S., Peking UniversityDirected by: Professor Meng-Lin Tsao Professor Tao YeIncorporation of unnatural amino acids into proteins… (more)

▼ ABSTRACTAdding New Chemistry to Proteins via Genetic IncorporationbyShuo Chen, B.S., Peking UniversityDirected by: Professor Meng-Lin Tsao Professor Tao YeIncorporation of unnatural amino acids into proteins provides a powerful way to add new chemistries to proteins, which would be an advantage to study protein behavior, to provide a site-specific protein modification method, and to enhance protein properties. Rather than incorporating unnatural amino acid into proteins by means of chemical methods, recent advances in genetic incorporation of unnatural amino acid into proteins might be an effective way to introduce novel chemical groups into proteins, which shows a promising future in chemical biology studies.The unnatural amino acid, 2-amino-3- (6-hydroxy-naphthalen-2-yl)propanoic acid (2NpOH) is an analogue of tyrosine, with an orthogonal reactivity and unique fluorescence properties, which can be utilized as site-specific bio-conjugation tag or as a biochemical probe. Based on rational design and random mutation, two aminoacyl-tRNA synthetases (RS-NpOH), which can be utilized to introduce 2-amino-3- (6-hydroxy-naphthalen-2-yl) propanoic acid into protein in vivo in response to the UAG stop codon, were evolved. Protein expression with RS-NpOH showed that the unnatural amino acid could be successfully incorporated into proteins. Both ESI-MS analysis and the strong blue fluorescence acquired by addition of 2NpOH indicated that both aminoacyl-tRNA synthetases had high efficiency and fidelity for selective incorporation of 2NpOH.A chemoselective azo coupling reaction between the genetically encoded 2-naphthol group and diazotized aniline derivatives was developed. The coupling reaction only required very mild condition of pH 7 at 0 °C, and possessed fast reaction rate, high efficiency and excellent selectivity. Thus, it provides us an alternate method for bio-conjugation, such as PEGylation. Furthermore, to exploit the higher reactivity of the 2-naphthol residue under a nucleophilic addition coupling, an in vivo Mannich type reaction was proposed. It revealed that a cross coupling reaction could achieve between a lysine residue and a 2NpOH residue with the presence of formaldehyde at physiological conditions. Thus, new functionality can be attached to the unnatural amino acid site of proteins, and it would provide us a new tool to study the behavior of proteins and eventually the production of functionally enhanced proteins.In addition, the fluorescent feature of this unnatural amino acid was also studied upon insertion of the 2NpOH into the GFP chromophore for protein evolution. However, no red-shift fluorescence was observed as expected In this work, incorporation of two unnatural amino acids into single protein was also demonstrated.

Subjects/Keywords: Biochemistry

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Chen, S. (2014). Adding new chemistry to proteins via genetic incorporation. (Thesis). University of California – Merced. Retrieved from http://www.escholarship.org/uc/item/9jb4g6jb

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Chen, Shuo. “Adding new chemistry to proteins via genetic incorporation.” 2014. Thesis, University of California – Merced. Accessed February 27, 2021. http://www.escholarship.org/uc/item/9jb4g6jb.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Chen, Shuo. “Adding new chemistry to proteins via genetic incorporation.” 2014. Web. 27 Feb 2021.

Vancouver:

Chen S. Adding new chemistry to proteins via genetic incorporation. [Internet] [Thesis]. University of California – Merced; 2014. [cited 2021 Feb 27]. Available from: http://www.escholarship.org/uc/item/9jb4g6jb.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Chen S. Adding new chemistry to proteins via genetic incorporation. [Thesis]. University of California – Merced; 2014. Available from: http://www.escholarship.org/uc/item/9jb4g6jb

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of California – Berkeley

26. Fernhoff, Nathaniel Bernard. On the Molecular Mechanisms of Functional and Dysfunction NO Signaling in Mammalian Physiology.

Degree: Molecular & Cell Biology, 2010, University of California – Berkeley

URL: http://www.escholarship.org/uc/item/2cq0g1wq

► Nitric oxide (NO) is an important physiological mediator of vasodilation, platelet aggregation, and neurotransmission. An NO signal regulates these processes by signal transduction through the… (more)

▼ Nitric oxide (NO) is an important physiological mediator of vasodilation, platelet aggregation, and neurotransmission. An NO signal regulates these processes by signal transduction through the enzyme soluble guanylate cyclase (sGC), and dysfunction in this pathway manifests in human disease. NO activates sGC several hundred fold to produce the second messenger cGMP, and the mechanism of activation was previously thought to result exclusively from NO binding to the sGC heme. However, recent studies have shown that heme-bound NO only partially activates sGC and additional NO binding to a nonheme site is required for maximal NO activation. Therefore, understanding the nature the nonheme-NO coordination is crucial to understanding the mechanism of enzyme activation.Thiol oxidation of sGC cysteines results in the loss of enzyme activity, therefore, the role of cysteines in NO-stimulated sGC activity was investigated. We found that the thiol modifying reagent methyl methanethiosulfonate specifically inhibits NO activation of sGC by blocking the non-heme site, which defines a role for sGC cysteine(s) in mediating NO binding. The nature of the NO/cysteine interaction was probed by examining the effects of redox active reagents on NO-stimulated activity. These results show that NO binding to, and dissociation from, the critical cysteine(s) does not involve a change in the thiol redox state. Evidence is provided for non-heme NO in the physiological activation of sGC in context of a primary cell culture of human umbilical vein endothelial cells. These findings have relevance to diseases involving the NO/cGMP signaling pathway.Heme-oxidation also results in the loss enzyme activity and may play a role in the NO-desensitization of sGC in cardiovascular disease. In vitro, the sGC heme is typically found in the ferrous [Fe(II)] state, which is activated several hundred fold by NO. Comparatively, the ferric [Fe(III)] form of sGC is only weakly activated by NO, but the mechanism of this blunted response is not well understood. Specifically, sGC undergoes a reaction with NO termed reductive nitrosylation, where the sGC heme is reduced to the ferrous [Fe(II)] state; but despite the recovery of the initial heme state, NO-stimulated activity does not recover. We found that the reduction of the sGC heme occurs via a coupled oxidation of an sGC cysteine to a nitrosothiol, and that the S-nitrosation of this key cysteine inhibits the enzymatic response to NO. The data from chapter 2 suggests the mechanism of inhibition may be the oxidation of a cysteine that constitutes the nonheme NO binding site. Thus, thiol-oxidation is at the root of the NO-desensitization of heme-oxidized sGC and the implications to human health and disease are discussed.

Subjects/Keywords: Biochemistry

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Fernhoff, N. B. (2010). On the Molecular Mechanisms of Functional and Dysfunction NO Signaling in Mammalian Physiology. (Thesis). University of California – Berkeley. Retrieved from http://www.escholarship.org/uc/item/2cq0g1wq

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Fernhoff, Nathaniel Bernard. “On the Molecular Mechanisms of Functional and Dysfunction NO Signaling in Mammalian Physiology.” 2010. Thesis, University of California – Berkeley. Accessed February 27, 2021. http://www.escholarship.org/uc/item/2cq0g1wq.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Fernhoff, Nathaniel Bernard. “On the Molecular Mechanisms of Functional and Dysfunction NO Signaling in Mammalian Physiology.” 2010. Web. 27 Feb 2021.

Vancouver:

Fernhoff NB. On the Molecular Mechanisms of Functional and Dysfunction NO Signaling in Mammalian Physiology. [Internet] [Thesis]. University of California – Berkeley; 2010. [cited 2021 Feb 27]. Available from: http://www.escholarship.org/uc/item/2cq0g1wq.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Fernhoff NB. On the Molecular Mechanisms of Functional and Dysfunction NO Signaling in Mammalian Physiology. [Thesis]. University of California – Berkeley; 2010. Available from: http://www.escholarship.org/uc/item/2cq0g1wq

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of California – Berkeley

27. Dunkle, Jack A. Structural Studies of the Elongation Cycle of Protein Synthesis and Its Inhibition by Antibiotics.

Degree: Molecular & Cell Biology, 2010, University of California – Berkeley

URL: http://www.escholarship.org/uc/item/49m7m9bs

► Protein synthesis takes place in four stages: initiation, elongation, termination and recycling. Elongation consists of delivery of a charged tRNA to the ribosome, peptide bond… (more)

Subjects/Keywords: Biochemistry

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Dunkle, J. A. (2010). Structural Studies of the Elongation Cycle of Protein Synthesis and Its Inhibition by Antibiotics. (Thesis). University of California – Berkeley. Retrieved from http://www.escholarship.org/uc/item/49m7m9bs

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Dunkle, Jack A. “Structural Studies of the Elongation Cycle of Protein Synthesis and Its Inhibition by Antibiotics.” 2010. Thesis, University of California – Berkeley. Accessed February 27, 2021. http://www.escholarship.org/uc/item/49m7m9bs.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Dunkle, Jack A. “Structural Studies of the Elongation Cycle of Protein Synthesis and Its Inhibition by Antibiotics.” 2010. Web. 27 Feb 2021.

Vancouver:

Dunkle JA. Structural Studies of the Elongation Cycle of Protein Synthesis and Its Inhibition by Antibiotics. [Internet] [Thesis]. University of California – Berkeley; 2010. [cited 2021 Feb 27]. Available from: http://www.escholarship.org/uc/item/49m7m9bs.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Dunkle JA. Structural Studies of the Elongation Cycle of Protein Synthesis and Its Inhibition by Antibiotics. [Thesis]. University of California – Berkeley; 2010. Available from: http://www.escholarship.org/uc/item/49m7m9bs

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of California – Berkeley

28. Shen, Sam. In search of a physiological role for amine oxidase,copper containing-3 (AOC3) in adipocytes.

Degree: Chemistry, 2010, University of California – Berkeley

URL: http://www.escholarship.org/uc/item/8br113n1

► In search of a physiological role for amine oxidase,copper containing-3 (AOC3) in adipocytesThe function of adipocytes was initially thought to be fat storage, thermal insulation,… (more)

▼ In search of a physiological role for amine oxidase,copper containing-3 (AOC3) in adipocytesThe function of adipocytes was initially thought to be fat storage, thermal insulation, and organprotection. With the discovery of adipokine, cytokine, and chemokine production by adipocytes,they are now considered to have endocrine function with a major role in homeostasis and energybalance. Amine oxidase, copper containing 3 (AOC3) is a major ectoenzyme expressed byadipocytes and catalyzes the oxidative deamination of amines to produce hydrogen peroxide,ammonia, and aldehyde. The physiological function of AOC3 in adipocytes and its endogenoussubstrate(s) are currently unknown. First, a signaling role for the hydrogen peroxide product wasinvestigated. Using an intracellular probe specific for H2O2, it was found that the plasmamembrane of adipocytes is permeable to H2O2 introduced exogenously. However, it was notpossible to detect an increase in intracellular H2O2 even after long incubation times with AOC3substrates. Adipocytes were found to produce extracellular H2O2 at an approximate rate of 0.078uM/min/well upon incubation with AOC3 substrate from whole cell kinetic studies. Due to thisslow rate of H2O2 production, the probe may not be sensitive enough to show an increase inintracellular H2O2 originating from AOC3 turnover. Adipocytes may have an immune function,with possible involvement in chemokine production. Using chemotaxis assays, the migration ofRAW264.7 macrophages was found to be independent of media components released byadipocytes incubated with isoamylamine, suggesting an absence or ineffectively lowconcentration of macrophage-specific chemokine release by adipocytes. However, theseexperiments used unactivated macrophages, which subsequently were reported by others to havechemotactic characteristics that are distinct from activated macrophages; the latter will be thesubject of future studies. In addition to the chemotaxis assay, release of the chemokines byadipocytes was investigated using ELISA. It was found that release of monocytechemoattractant protein-1 (MCP-1) by adipocytes was dependent on both concentration of H2O2exposure and incubation time, with adipocytes also capable of constitutive MCP-1 release.However, the release of MCP-1, as well as other cytokines (including IL-6, KC, and IP-10), wasfound to have little correlation with AOC3 turnover. On the other hand, release of IL-13, a cytokine correlated with populations of alternatively activated macrophages, was shown to bedependent on AOC3 turnover, though the increase was low with no correlation to H2O2. To findadipocyte response(s) to AOC3 turnover, it is important to characterize the enzyme's kineticprofile, since the endogenous substrate(s) of AOC3 is currently unknown. With this goal inmind, the human AOC3 enzyme was expressed and purified using a new expression system andpurification protocol. Using the purified enzyme, a substrate kinetic profile was determined. Itwas found that both cyclic and noncyclic alkylamines, arylamines, and branched…

Subjects/Keywords: Biochemistry

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Shen, S. (2010). In search of a physiological role for amine oxidase,copper containing-3 (AOC3) in adipocytes. (Thesis). University of California – Berkeley. Retrieved from http://www.escholarship.org/uc/item/8br113n1

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Shen, Sam. “In search of a physiological role for amine oxidase,copper containing-3 (AOC3) in adipocytes.” 2010. Thesis, University of California – Berkeley. Accessed February 27, 2021. http://www.escholarship.org/uc/item/8br113n1.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Shen, Sam. “In search of a physiological role for amine oxidase,copper containing-3 (AOC3) in adipocytes.” 2010. Web. 27 Feb 2021.

Vancouver:

Shen S. In search of a physiological role for amine oxidase,copper containing-3 (AOC3) in adipocytes. [Internet] [Thesis]. University of California – Berkeley; 2010. [cited 2021 Feb 27]. Available from: http://www.escholarship.org/uc/item/8br113n1.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Shen S. In search of a physiological role for amine oxidase,copper containing-3 (AOC3) in adipocytes. [Thesis]. University of California – Berkeley; 2010. Available from: http://www.escholarship.org/uc/item/8br113n1

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of California – Irvine

29. Mathur, Radhika. Identification of a Novel Mechanism for Regulation of F-box Proteins.

Degree: Biomedical Sciences, 2014, University of California – Irvine

URL: http://www.escholarship.org/uc/item/2q49r7dz

► Post-translational modification of proteins with ubiquitin plays a role in most biological processes. The key players of this system are the E3 ubiquitin ligases, which… (more)

▼ Post-translational modification of proteins with ubiquitin plays a role in most biological processes. The key players of this system are the E3 ubiquitin ligases, which mediate substrate specific covalent attachment of ubiquitin. The SCF ubiquitin ligases are amongst best-understood E3 complexes. They are composed of Skp1, Cdc53/Cullin, Rbx1, and one of the multiple F-box proteins, which bind substrates and confer specificity to this complex. As these modular ligases share the same core components but differ in the identity of F-box proteins, the repertoire of SCF ligases present in a cell at any given time is thus governed by the abundance of different F-box proteins. Regulating F-box protein abundance is thus essential for normal cellular function and misregulation causes human diseases. In the studies presented here, we describe a novel mechanism governing maintenance of F-box protein homeostasis. In the absence of substrates, the core SCF ligase ubiquitylates its F-box protein and targets it for degradation. Using the yeast F-box protein Met30 as a model system, we demonstrate presence of an additional mechanism for F-box protein degradation apart from autoubiquitylation. This pathway targets F-box proteins that are dissociated from Skp1, and plays a crucial role in limiting substrate shielding effects caused by association of excess F-box proteins with their substrates thereby inhibiting substrate recognition by fully assembled ligases. Characterization of this pathway shows that it is governed by the ubiquitin proteasome system and is mediated by a ligase dependent on Cdc53 but independent of Skp1 and for the first time illustrates uncoupling of Cdc53 and Skp1 function in yeast.Furthermore, we show that Cic1 and the AAA ATPase Cdc48, along with its substrate recruiting factors Npl4/Ufd1 are involved in this mechanism of F-box protein degradation. Preliminary studies conducted to ascribe a role for these proteins in this process, suggest Cic1 as a unique factor that recruits ubiquitylated F-box proteins to Cdc48/Npl4/Ufd1 for targeting to proteasome for degradation. Together, our findings shed light on the complexity of the ubiquitin system. They uncover a novel degradation pathway, suggest existence of yet unknown types of ligases, and introduce the concept of protein specificity factors for Cdc48.

Subjects/Keywords: Biochemistry

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mathur, R. (2014). Identification of a Novel Mechanism for Regulation of F-box Proteins. (Thesis). University of California – Irvine. Retrieved from http://www.escholarship.org/uc/item/2q49r7dz

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mathur, Radhika. “Identification of a Novel Mechanism for Regulation of F-box Proteins.” 2014. Thesis, University of California – Irvine. Accessed February 27, 2021. http://www.escholarship.org/uc/item/2q49r7dz.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mathur, Radhika. “Identification of a Novel Mechanism for Regulation of F-box Proteins.” 2014. Web. 27 Feb 2021.

Vancouver:

Mathur R. Identification of a Novel Mechanism for Regulation of F-box Proteins. [Internet] [Thesis]. University of California – Irvine; 2014. [cited 2021 Feb 27]. Available from: http://www.escholarship.org/uc/item/2q49r7dz.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mathur R. Identification of a Novel Mechanism for Regulation of F-box Proteins. [Thesis]. University of California – Irvine; 2014. Available from: http://www.escholarship.org/uc/item/2q49r7dz

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of California – Santa Cruz

30. Jameson, John Brian. Characterization and Inhibition Studies of 15-Lipoxygenase Enzymes.

Degree: Chemistry, 2014, University of California – Santa Cruz

URL: http://www.escholarship.org/uc/item/67w3t326

► The research presented in this dissertation focuses on two distinct areas of 15-lipoxygenase biology: screening and identification of inhibitors toward human 15-lipoxygenase enzymes and biological… (more)

▼ The research presented in this dissertation focuses on two distinct areas of 15-lipoxygenase biology: screening and identification of inhibitors toward human 15-lipoxygenase enzymes and biological and biochemical characterization of a 15-lipoxygenase enzyme (p15-LOX) from the opportunistic pathogen Pseudomonas aeruginosa (P. aeruginosa). Two novel, selective inhibitors are reported for human 15-lipoxygenase-2 (15-LOX-2). The reported compounds were micromolar active with competitive and mixed type inhibition. They will serve as chemical tools for future studies to more deeply probe the role of 15-LOX-2 in atherosclerosis, cancer, and for general elucidation of 15-LOX-2 function in tissues. In addition, the binding affinities for five nanomolar active human 15-lipoxygenase-1 inhibitors were determined by a novel mass spectrometric assay with the ability to measure binding affinities well below the current limit of quantitation for the traditional UV assay. The assay also represents the first of its kind capable of assessing allosteric effect in a high throughput, 96-well format, opening the possibility of screening compound libraries to identify allosteric effectors to lipoxygenase (LOX) enzymes. Finally, a number of biochemical and biological properties of the LOX enzyme expressed by P. aeruginosa were determined. The most actively metabolized substrate is AA and K_cat far exceeds human homologues at low pH. The enzyme is active with a variety of polyunsaturated fatty acid substrates, but phospholipids are not metabolized well compared to human LOX enzymes. Selective inhibitors to human LOX isozymes do not significantly inhibit p15-LOX. Expression of LOX during P. aeruginosa infection increases observed cytokines from several cell types. Together these studies support the hypothesis that this enzyme is capable of disrupting host immunity, but do not strongly support expression of p15-LOX solely for this purpose. Subsequent studies are needed to further elucidate the biological function of p15-LOX.

Subjects/Keywords: Biochemistry

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Jameson, J. B. (2014). Characterization and Inhibition Studies of 15-Lipoxygenase Enzymes. (Thesis). University of California – Santa Cruz. Retrieved from http://www.escholarship.org/uc/item/67w3t326

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Jameson, John Brian. “Characterization and Inhibition Studies of 15-Lipoxygenase Enzymes.” 2014. Thesis, University of California – Santa Cruz. Accessed February 27, 2021. http://www.escholarship.org/uc/item/67w3t326.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Jameson, John Brian. “Characterization and Inhibition Studies of 15-Lipoxygenase Enzymes.” 2014. Web. 27 Feb 2021.

Vancouver:

Jameson JB. Characterization and Inhibition Studies of 15-Lipoxygenase Enzymes. [Internet] [Thesis]. University of California – Santa Cruz; 2014. [cited 2021 Feb 27]. Available from: http://www.escholarship.org/uc/item/67w3t326.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Jameson JB. Characterization and Inhibition Studies of 15-Lipoxygenase Enzymes. [Thesis]. University of California – Santa Cruz; 2014. Available from: http://www.escholarship.org/uc/item/67w3t326

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Open Access Theses and Dissertations