subject:(bacteriology)

Eastern Illinois University

1. Paul, Oindrila. Resolving the Nutritional Requirements of the Bile Acid-Dehydroxylating Gut Bacterium Clostridium scindens.

Degree: MS, Biological Sciences, 2013, Eastern Illinois University

URL: https://thekeep.eiu.edu/theses/1181

► Compared to the other parts of the human body, the gastrointestinal tract harbors a complex microbiota. The impact of the indigenous microbiota on host… (more)

▼ Compared to the other parts of the human body, the gastrointestinal tract harbors a complex microbiota. The impact of the indigenous microbiota on host physiology is most pronounced in the colon, where the primary bile acids chenodeoxycholic acid and cholic acid are converted, via 7α-dehydroxylation, to the toxic secondary bile acids lithocholic acid and deoxycholic acid, respectively, by Clostridium scindens, an obligate anaerobe. Interestingly, other than bile acid dehydroxylation, little is known about the basic physiology of C. scindens. Understanding the metabolism of C. scindens will hopefully provide much-needed information as to how this gut bacterium impacts human health and disease. The goals of our study were to determine the vitamin and amino acid requirements of C. scindens and also to determine if C. scindens can convert primary bile acid cholic acid to secondary bile acid deoxycholic acid via 7α-dehydroxylation under defined culture conditions. C. scindens VPI 12708 was routinely maintained in anaerobic BHI broth medium at 37°C. When C. scindens was transferred from the BHI medium to a defined medium (DM; 25 mM glucose, minerals, metals, bicarbonate, 100% CO₂ gas phase, and cysteine), growth was negligible. Only when DM was supplemented with a vitamin mix (p-aminobenzoate, biotin, cyanocobalamin, folate, lipoate, nicotinate, pantothenate, pyridoxal, riboflavin, and thiamine) and an amino acid mix (alanine, arginine, asparagine, aspartate, cystine, glutamate, glutamine, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine, and valine) was growth observed. The leave-one-out technique was subsequently used to determine the specific vitamin(s) and amino acid(s) required for growth. With this technique, two vitamins (riboflavin and pantothenate) and one amino acid (tryptophan) were found to be essential for the growth of C. scindens. Growth was also reduced, but not inhibited when pyridoxal or biotin was absent from OM. It was also observed that under defined culture conditions C. scindens was able to convert cholic acid to deoxycholic acid via 7α-dehydroxylation while producing two unknown products. Advisors/Committee Members: Steven L. Daniel, Gary A. Bulla, Kai Hung.

Subjects/Keywords: Bacteriology

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APA (6^th Edition):

Paul, O. (2013). Resolving the Nutritional Requirements of the Bile Acid-Dehydroxylating Gut Bacterium Clostridium scindens. (Masters Thesis). Eastern Illinois University. Retrieved from https://thekeep.eiu.edu/theses/1181

Chicago Manual of Style (16^th Edition):

Paul, Oindrila. “Resolving the Nutritional Requirements of the Bile Acid-Dehydroxylating Gut Bacterium Clostridium scindens.” 2013. Masters Thesis, Eastern Illinois University. Accessed April 14, 2021. https://thekeep.eiu.edu/theses/1181.

MLA Handbook (7^th Edition):

Paul, Oindrila. “Resolving the Nutritional Requirements of the Bile Acid-Dehydroxylating Gut Bacterium Clostridium scindens.” 2013. Web. 14 Apr 2021.

Vancouver:

Paul O. Resolving the Nutritional Requirements of the Bile Acid-Dehydroxylating Gut Bacterium Clostridium scindens. [Internet] [Masters thesis]. Eastern Illinois University; 2013. [cited 2021 Apr 14]. Available from: https://thekeep.eiu.edu/theses/1181.

Council of Science Editors:

Paul O. Resolving the Nutritional Requirements of the Bile Acid-Dehydroxylating Gut Bacterium Clostridium scindens. [Masters Thesis]. Eastern Illinois University; 2013. Available from: https://thekeep.eiu.edu/theses/1181

McMaster University

2. Sharthiya, Harsh. THE ROLE OF THE PHOU PROTEIN IN SINORHIZOBIUM MELILOTI.

Degree: MSc, 2013, McMaster University

URL: http://hdl.handle.net/11375/15299

►

Phosphate is of central importance in cellular metabolism and since bacteria are often exposed to various concentration of phosphorous in their environment, they have… (more)

Subjects/Keywords: PhoU; Sinorhizobium meliloti; Bacteriology; Bacteriology

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APA (6^th Edition):

Sharthiya, H. (2013). THE ROLE OF THE PHOU PROTEIN IN SINORHIZOBIUM MELILOTI. (Masters Thesis). McMaster University. Retrieved from http://hdl.handle.net/11375/15299

Chicago Manual of Style (16^th Edition):

Sharthiya, Harsh. “THE ROLE OF THE PHOU PROTEIN IN SINORHIZOBIUM MELILOTI.” 2013. Masters Thesis, McMaster University. Accessed April 14, 2021. http://hdl.handle.net/11375/15299.

MLA Handbook (7^th Edition):

Sharthiya, Harsh. “THE ROLE OF THE PHOU PROTEIN IN SINORHIZOBIUM MELILOTI.” 2013. Web. 14 Apr 2021.

Vancouver:

Sharthiya H. THE ROLE OF THE PHOU PROTEIN IN SINORHIZOBIUM MELILOTI. [Internet] [Masters thesis]. McMaster University; 2013. [cited 2021 Apr 14]. Available from: http://hdl.handle.net/11375/15299.

Council of Science Editors:

Sharthiya H. THE ROLE OF THE PHOU PROTEIN IN SINORHIZOBIUM MELILOTI. [Masters Thesis]. McMaster University; 2013. Available from: http://hdl.handle.net/11375/15299

Universidad de Cantabria

3. Echavarri Erasun, Beatriz. Estudio de los efectos sobre el medio litoral derivados de la implantación de saneamientos integrales en la costa cantábrica.

Degree: 2007, Universidad de Cantabria

URL: http://hdl.handle.net/10902/1307

► RESUMEN: La presente tesis "estudio de los efectos sobre el medio litoral derivados de la implantación de saneamientos integrales en la costa Cantábrica" pretende evaluar… (more)

Subjects/Keywords: Bacteriology

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APA (6^th Edition):

Echavarri Erasun, B. (2007). Estudio de los efectos sobre el medio litoral derivados de la implantación de saneamientos integrales en la costa cantábrica. (Doctoral Dissertation). Universidad de Cantabria. Retrieved from http://hdl.handle.net/10902/1307

Chicago Manual of Style (16^th Edition):

Echavarri Erasun, Beatriz. “Estudio de los efectos sobre el medio litoral derivados de la implantación de saneamientos integrales en la costa cantábrica.” 2007. Doctoral Dissertation, Universidad de Cantabria. Accessed April 14, 2021. http://hdl.handle.net/10902/1307.

MLA Handbook (7^th Edition):

Echavarri Erasun, Beatriz. “Estudio de los efectos sobre el medio litoral derivados de la implantación de saneamientos integrales en la costa cantábrica.” 2007. Web. 14 Apr 2021.

Vancouver:

Echavarri Erasun B. Estudio de los efectos sobre el medio litoral derivados de la implantación de saneamientos integrales en la costa cantábrica. [Internet] [Doctoral dissertation]. Universidad de Cantabria; 2007. [cited 2021 Apr 14]. Available from: http://hdl.handle.net/10902/1307.

Council of Science Editors:

Echavarri Erasun B. Estudio de los efectos sobre el medio litoral derivados de la implantación de saneamientos integrales en la costa cantábrica. [Doctoral Dissertation]. Universidad de Cantabria; 2007. Available from: http://hdl.handle.net/10902/1307

University of Minnesota

4. Green, Beryl Sparks. The Oxygen Content of Media in Relation to Surface Tension and Bacterial Growth.

Degree: Masters, Bacteriology, 1921, University of Minnesota

URL: http://hdl.handle.net/11299/179541

Subjects/Keywords: Bacteriology

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APA (6^th Edition):

Green, B. S. (1921). The Oxygen Content of Media in Relation to Surface Tension and Bacterial Growth. (Masters Thesis). University of Minnesota. Retrieved from http://hdl.handle.net/11299/179541

Chicago Manual of Style (16^th Edition):

Green, Beryl Sparks. “The Oxygen Content of Media in Relation to Surface Tension and Bacterial Growth.” 1921. Masters Thesis, University of Minnesota. Accessed April 14, 2021. http://hdl.handle.net/11299/179541.

MLA Handbook (7^th Edition):

Green, Beryl Sparks. “The Oxygen Content of Media in Relation to Surface Tension and Bacterial Growth.” 1921. Web. 14 Apr 2021.

Vancouver:

Green BS. The Oxygen Content of Media in Relation to Surface Tension and Bacterial Growth. [Internet] [Masters thesis]. University of Minnesota; 1921. [cited 2021 Apr 14]. Available from: http://hdl.handle.net/11299/179541.

Council of Science Editors:

Green BS. The Oxygen Content of Media in Relation to Surface Tension and Bacterial Growth. [Masters Thesis]. University of Minnesota; 1921. Available from: http://hdl.handle.net/11299/179541

Eastern Illinois University

5. Shrestha, Rachana. Resolution of the Nutritional Requirements and Metabolic Profile of Clostridium scindens ATCC 35704, A Major Bile Acid-Dehydroxylating Anaerobe in the Human Gut.

Degree: Biological Sciences, 2018, Eastern Illinois University

URL: https://thekeep.eiu.edu/theses/4420

► Bile acids (cholate and chenodeoxycholate) are synthesized from cholesterol in the human liver, and secreted in small intestine for food digestion. However, a portion… (more)

Subjects/Keywords: Bacteriology; Nutrition

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APA (6^th Edition):

Shrestha, R. (2018). Resolution of the Nutritional Requirements and Metabolic Profile of Clostridium scindens ATCC 35704, A Major Bile Acid-Dehydroxylating Anaerobe in the Human Gut. (Thesis). Eastern Illinois University. Retrieved from https://thekeep.eiu.edu/theses/4420

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Shrestha, Rachana. “Resolution of the Nutritional Requirements and Metabolic Profile of Clostridium scindens ATCC 35704, A Major Bile Acid-Dehydroxylating Anaerobe in the Human Gut.” 2018. Thesis, Eastern Illinois University. Accessed April 14, 2021. https://thekeep.eiu.edu/theses/4420.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Shrestha, Rachana. “Resolution of the Nutritional Requirements and Metabolic Profile of Clostridium scindens ATCC 35704, A Major Bile Acid-Dehydroxylating Anaerobe in the Human Gut.” 2018. Web. 14 Apr 2021.

Vancouver:

Shrestha R. Resolution of the Nutritional Requirements and Metabolic Profile of Clostridium scindens ATCC 35704, A Major Bile Acid-Dehydroxylating Anaerobe in the Human Gut. [Internet] [Thesis]. Eastern Illinois University; 2018. [cited 2021 Apr 14]. Available from: https://thekeep.eiu.edu/theses/4420.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Shrestha R. Resolution of the Nutritional Requirements and Metabolic Profile of Clostridium scindens ATCC 35704, A Major Bile Acid-Dehydroxylating Anaerobe in the Human Gut. [Thesis]. Eastern Illinois University; 2018. Available from: https://thekeep.eiu.edu/theses/4420

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Eastern Illinois University

6. Miller, Jill K. The Isolation and Partial Characterization of the Lipopolysaccharides from Rhizobium leguminosarum biovar trifolii strains TB4, TB104, and TB112.

Degree: MS, 1990, Eastern Illinois University

URL: https://thekeep.eiu.edu/theses/2302

► The lipopolysaccharides (LPSs) from Rhizobium leguminosarum biovar trifolii TB4 and two transposon (Tn5) symbiotic mutants, R. leguminosarum bv. trifolii TB104 and TB112, were isolated… (more)

Subjects/Keywords: Bacteriology

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APA (6^th Edition):

Miller, J. K. (1990). The Isolation and Partial Characterization of the Lipopolysaccharides from Rhizobium leguminosarum biovar trifolii strains TB4, TB104, and TB112. (Masters Thesis). Eastern Illinois University. Retrieved from https://thekeep.eiu.edu/theses/2302

Chicago Manual of Style (16^th Edition):

Miller, Jill K. “The Isolation and Partial Characterization of the Lipopolysaccharides from Rhizobium leguminosarum biovar trifolii strains TB4, TB104, and TB112.” 1990. Masters Thesis, Eastern Illinois University. Accessed April 14, 2021. https://thekeep.eiu.edu/theses/2302.

MLA Handbook (7^th Edition):

Miller, Jill K. “The Isolation and Partial Characterization of the Lipopolysaccharides from Rhizobium leguminosarum biovar trifolii strains TB4, TB104, and TB112.” 1990. Web. 14 Apr 2021.

Vancouver:

Miller JK. The Isolation and Partial Characterization of the Lipopolysaccharides from Rhizobium leguminosarum biovar trifolii strains TB4, TB104, and TB112. [Internet] [Masters thesis]. Eastern Illinois University; 1990. [cited 2021 Apr 14]. Available from: https://thekeep.eiu.edu/theses/2302.

Council of Science Editors:

Miller JK. The Isolation and Partial Characterization of the Lipopolysaccharides from Rhizobium leguminosarum biovar trifolii strains TB4, TB104, and TB112. [Masters Thesis]. Eastern Illinois University; 1990. Available from: https://thekeep.eiu.edu/theses/2302

Eastern Illinois University

7. Wozniak, Keith Leonard. Antibiotic Effects on Coliform Enumeration.

Degree: MS, 1987, Eastern Illinois University

URL: https://thekeep.eiu.edu/theses/2595

► The inhibitory effects of Bacillus polymyxa on Escherichia coli were studied. Significant coliform inhibition was observed. The inhibitory agent (presumably polymyxin) was active against… (more)

Subjects/Keywords: Bacteriology

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APA (6^th Edition):

Wozniak, K. L. (1987). Antibiotic Effects on Coliform Enumeration. (Masters Thesis). Eastern Illinois University. Retrieved from https://thekeep.eiu.edu/theses/2595

Chicago Manual of Style (16^th Edition):

Wozniak, Keith Leonard. “Antibiotic Effects on Coliform Enumeration.” 1987. Masters Thesis, Eastern Illinois University. Accessed April 14, 2021. https://thekeep.eiu.edu/theses/2595.

MLA Handbook (7^th Edition):

Wozniak, Keith Leonard. “Antibiotic Effects on Coliform Enumeration.” 1987. Web. 14 Apr 2021.

Vancouver:

Wozniak KL. Antibiotic Effects on Coliform Enumeration. [Internet] [Masters thesis]. Eastern Illinois University; 1987. [cited 2021 Apr 14]. Available from: https://thekeep.eiu.edu/theses/2595.

Council of Science Editors:

Wozniak KL. Antibiotic Effects on Coliform Enumeration. [Masters Thesis]. Eastern Illinois University; 1987. Available from: https://thekeep.eiu.edu/theses/2595

Eastern Illinois University

8. Shatters, Robert G., Jr. Characterization of the Lipopolysaccharides (LPS) from Four Transposon Generated Symbiotic Mutants of Rhizobium trifolii.

Degree: MS, 1984, Eastern Illinois University

URL: https://thekeep.eiu.edu/theses/2792

► The LPSs from four symbiotic mutants of R. trifolii were isolated and characterized. The mutants were obtained from Dr. Barry Rolfe of The Australian… (more)

▼ The LPSs from four symbiotic mutants of R. trifolii were isolated and characterized. The mutants were obtained from Dr. Barry Rolfe of The Australian National University. Three mutants have Tn5 insertions in the "nod" regions of the symbiotic plasmid. The fourth has a 30 kb deletion. This deletion includes the majority of the "nod" regions. All mutants fail to nodulate clover and fail to induce markedly curled root hairs, i.e. they are hac-nod-. The LPSs were purified as described by Carlson et al. 1978.⁷ The polysaccharide portion of the LPSs was purified by treating the LPSs in 1% HAc at 100° for 1 hour followed by Sephadex G-50 column chromatography. A larger and a smaller molecular weight polysaccharide (LPS1 and LPS2) are obtained. The LPS from one batch, but not from a second batch, of the insertion mutant ANU274 contains an additional polysaccharide which elutes in the void volume of the G-50 column (LPS3). Sugar compositions were determined by GC analysis of their alditol acetate derivatives. Uronic acid, 2-keto-3-deoxyoctonic acid (KDO), pyruvate and acetate were determined by colorimetric assays. Unusual sugars were identified by combined GC/MS analysis. The LPSs were also analyzed by PAGE.²¹ The LPS compositions from the mutants and the parent are similar, the major sugars being heptose galacturonic acid and glucuronic acid. The LPS from one batch of ANU274 was different in that it also contains rhamnose (not found in the other LPSs), increased levels of galactose and an unidentified molecule. The LPS from a second batch of ANU274 is the same as the parent and other mutant LPSs. The LPS1s from all strains, including both batches of ANU274, are very similar in composition. The LPS2s contain man, gal, glc and uronic acid, the major sugar being uronic acid. The LPS2s from all the mutants contain increased levels of glucose compared to the parent. We are uncertain if this glucose is an LPS2 component or due to contamination by small molecular weight glucans. The LPS3, present in the one batch of ANU274, contains largely rhamnose and galactose. PAGE analysis of the various LPSs show that they are very similar, except for the LPS from the one batch of ANU274. The banding patterns, except that of the one ANU274 LPS, suggest that the O-antigen may not consist of a repeating oligosaccharide but may be a single complex oligosaccharide. However, the banding pattern for the LPS from the one batch of ANU274 suggests an O-antigen which does consist of a repeating oligosaccharide. At the present time we do not know if the appearance of this different LPS in the one batch of ANU274 is due to the mutation and/or is due to slightly different growth conditions (although all batches were grown and harvested in a similar manner). Advisors/Committee Members: Terry M. Weidner.

Subjects/Keywords: Bacteriology

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APA (6^th Edition):

Shatters, Robert G., J. (1984). Characterization of the Lipopolysaccharides (LPS) from Four Transposon Generated Symbiotic Mutants of Rhizobium trifolii. (Masters Thesis). Eastern Illinois University. Retrieved from https://thekeep.eiu.edu/theses/2792

Chicago Manual of Style (16^th Edition):

Shatters, Robert G., Jr. “Characterization of the Lipopolysaccharides (LPS) from Four Transposon Generated Symbiotic Mutants of Rhizobium trifolii.” 1984. Masters Thesis, Eastern Illinois University. Accessed April 14, 2021. https://thekeep.eiu.edu/theses/2792.

MLA Handbook (7^th Edition):

Shatters, Robert G., Jr. “Characterization of the Lipopolysaccharides (LPS) from Four Transposon Generated Symbiotic Mutants of Rhizobium trifolii.” 1984. Web. 14 Apr 2021.

Vancouver:

Shatters, Robert G. J. Characterization of the Lipopolysaccharides (LPS) from Four Transposon Generated Symbiotic Mutants of Rhizobium trifolii. [Internet] [Masters thesis]. Eastern Illinois University; 1984. [cited 2021 Apr 14]. Available from: https://thekeep.eiu.edu/theses/2792.

Council of Science Editors:

Shatters, Robert G. J. Characterization of the Lipopolysaccharides (LPS) from Four Transposon Generated Symbiotic Mutants of Rhizobium trifolii. [Masters Thesis]. Eastern Illinois University; 1984. Available from: https://thekeep.eiu.edu/theses/2792

Eastern Illinois University

9. Swanson, Ray W. Siderocapsa Major – Fact or Artifact?.

Degree: MS, 1979, Eastern Illinois University

URL: https://thekeep.eiu.edu/theses/3120

► The validity of the genus Siderocapsa, a group of unicellular iron-depositing bacteria, has been a subject of debate for many years. Lack of photomicroscopic… (more)

▼ The validity of the genus Siderocapsa, a group of unicellular iron-depositing bacteria, has been a subject of debate for many years. Lack of photomicroscopic and cultural studies has kept this group in taxonomic obscurity. Bacteria were collected from the Campus pond, Eastern Illinois University, Charleston, Illinois, by a submerged slide/coverslip technique. These bacteria were identified as Siderocapsa major according to the description of Molisch (1909). Scanning electron microscopy of Siderocapsa major microcolonies has shown cells (0.5 by 1.15 μm), in a clear central well (2-6 μm), surrounded by an amorphous material which accumulates with the age of the colony. Most authors have failed to explain how they have concluded that the material sequestered by Siderocapsa microcolonies is iron and/or manganese. Preliminary work employing the Prussian blue Reaction (and modifications) failed to demonstrate the presence of iron in the capsules around these bacteria. X-ray microanalysis also failed to demonstrate the presence of iron. However significant amounts of manganese were found to be present in the extra-cellular material. Some investigators have suggested that Siderocapsa microcolonies are artifacts and the result of rod-shaped bacteria that have become entrapped in the well of a Sphaerotilus natans holdfast whose filament has become dislodged. Scanning electron microscopy has shown that the size and morphology of the holdfast of Sphaerotilus-Leptothrix members does not correspond to the size of the deposit around the Siderocapsa microcapsule. In addition, statistical analysis has shown a definite developmental pattern relating the total number of cells in the microcapsule to the total amount of material deposited. The author concludes, from material observed here, that the Siderocapsa microcolonies are discretely different entities from members of the Sphaerotilus-Leptothrix group but may be related to other heterotrophic bacteria. Advisors/Committee Members: William A. Weiler.

Subjects/Keywords: Bacteriology

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APA (6^th Edition):

Swanson, R. W. (1979). Siderocapsa Major – Fact or Artifact?. (Masters Thesis). Eastern Illinois University. Retrieved from https://thekeep.eiu.edu/theses/3120

Chicago Manual of Style (16^th Edition):

Swanson, Ray W. “Siderocapsa Major – Fact or Artifact?.” 1979. Masters Thesis, Eastern Illinois University. Accessed April 14, 2021. https://thekeep.eiu.edu/theses/3120.

MLA Handbook (7^th Edition):

Swanson, Ray W. “Siderocapsa Major – Fact or Artifact?.” 1979. Web. 14 Apr 2021.

Vancouver:

Swanson RW. Siderocapsa Major – Fact or Artifact?. [Internet] [Masters thesis]. Eastern Illinois University; 1979. [cited 2021 Apr 14]. Available from: https://thekeep.eiu.edu/theses/3120.

Council of Science Editors:

Swanson RW. Siderocapsa Major – Fact or Artifact?. [Masters Thesis]. Eastern Illinois University; 1979. Available from: https://thekeep.eiu.edu/theses/3120

Eastern Illinois University

10. Pareek, Nivedita. Evaluation of Intra-Species Diversity of Oxalobacter formigenes Strains Using Pulsed-Field Gel Electrophoresis (PFGE) and Multiplex PCR.

Degree: MS, Biological Sciences, 2019, Eastern Illinois University

URL: https://thekeep.eiu.edu/theses/4580

► Oxalobacter formigenes is a beneficial, strict anaerobic gut bacterium that plays an important role in the prevention of kidney stone disease with a unique… (more)

▼ Oxalobacter formigenes is a beneficial, strict anaerobic gut bacterium that plays an important role in the prevention of kidney stone disease with a unique characteristic of using only oxalate as a carbon and energy source. To date, twenty-one strains of O. formigenes have been isolated which are further divided into two groups based on differences in the patterns of cell membrane lipids, cellular proteins, and nucleic acid fragments. The objective of the study was to evaluate intra-species diversity of O. formigenes strains using pulsed-field gel electrophoresis (PFGE) and multiplex PCR. Nine pure cultures of O. formigenes strains (5 strains group 1 specific; 4 strains group 2 specific), five O. formigenes human isolates (2 isolates group 1 specific; 3 isolates group 2 specific), and three O. formigenes mouse isolates (group 1 specific) were grown anaerobically at 37°C in undefined oxalate broth. Upon reaching late exponential growth, bacterial cells were harvested and added to melted agarose to make plugs which were incubated (55°C) in proteinase K solution to lyse cells, sliced, and then digested with Xbal 1 at 37°C. Digested agarose slices were loaded onto 1% Seakem Gold agarose gels, loaded onto PFGE for 18-19 h, and analyzed by BioNumerics software to generate a dendrogram. For multiplex PCR, bacterial cells were subjected to DNA extraction followed by amplification with suitable species and strain specific primers. In case of PFGE, four group 1 specific strains (isolated from human feces, sheep rumen, and fresh water lake sediment) showed a tight clustering with each other while other group 1 specific strain (isolated from wild rat) was tightly associated with group 1 specific human isolates. Three group 2 specific strains (isolated from human feces and Guinea pig) showed high similarity with each other but one group 2 specific strain (isolated from human feces) was in proximity with group 1 specific strains. Mouse isolates were compactly grouped with each other but did not show any closeness with rest of the strains. Amplification of genomic DNA obtained from O. formigenes strains and isolates by multiplex PCR showed the following products (amplicons): 416 bp for O. formigenes groups 1 and 2 (species specific); 210 bp for O. formigenes group 1 (group 1 specific); and 140 bp for O. formigenes group 2 (group 2 specific). Multiplex PCR of mixed bacterial DNA samples, containing high or low DNA concentrations of each group, yielded the expected 3 amplicons. This study was in overall agreement with the grouping of the strains with some exceptions indicating PFGE is a useful tool to study the phylogenetic relationship among O. formigenes strains. The multiplex PCR system for the one step detection and differentiation of O. formigenes strains is novel and optimized at every possible step asserting it as a unique and time saving technique in clinical field. Advisors/Committee Members: Steven L. Daniel, Kai Hung, Gary A. Bulla.

Subjects/Keywords: Bacteriology; Biodiversity

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APA (6^th Edition):

Pareek, N. (2019). Evaluation of Intra-Species Diversity of Oxalobacter formigenes Strains Using Pulsed-Field Gel Electrophoresis (PFGE) and Multiplex PCR. (Masters Thesis). Eastern Illinois University. Retrieved from https://thekeep.eiu.edu/theses/4580

Chicago Manual of Style (16^th Edition):

Pareek, Nivedita. “Evaluation of Intra-Species Diversity of Oxalobacter formigenes Strains Using Pulsed-Field Gel Electrophoresis (PFGE) and Multiplex PCR.” 2019. Masters Thesis, Eastern Illinois University. Accessed April 14, 2021. https://thekeep.eiu.edu/theses/4580.

MLA Handbook (7^th Edition):

Pareek, Nivedita. “Evaluation of Intra-Species Diversity of Oxalobacter formigenes Strains Using Pulsed-Field Gel Electrophoresis (PFGE) and Multiplex PCR.” 2019. Web. 14 Apr 2021.

Vancouver:

Pareek N. Evaluation of Intra-Species Diversity of Oxalobacter formigenes Strains Using Pulsed-Field Gel Electrophoresis (PFGE) and Multiplex PCR. [Internet] [Masters thesis]. Eastern Illinois University; 2019. [cited 2021 Apr 14]. Available from: https://thekeep.eiu.edu/theses/4580.

Council of Science Editors:

Pareek N. Evaluation of Intra-Species Diversity of Oxalobacter formigenes Strains Using Pulsed-Field Gel Electrophoresis (PFGE) and Multiplex PCR. [Masters Thesis]. Eastern Illinois University; 2019. Available from: https://thekeep.eiu.edu/theses/4580

Kansas State University

11. McDaniel, Lloyd Everett. A study of certain Salmonella and similar bacteria from the intestine of the chicken.

Degree: 1937, Kansas State University

URL: http://hdl.handle.net/2097/18057

Subjects/Keywords: Bacteriology

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APA (6^th Edition):

McDaniel, L. E. (1937). A study of certain Salmonella and similar bacteria from the intestine of the chicken. (Thesis). Kansas State University. Retrieved from http://hdl.handle.net/2097/18057

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

McDaniel, Lloyd Everett. “A study of certain Salmonella and similar bacteria from the intestine of the chicken.” 1937. Thesis, Kansas State University. Accessed April 14, 2021. http://hdl.handle.net/2097/18057.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

McDaniel, Lloyd Everett. “A study of certain Salmonella and similar bacteria from the intestine of the chicken.” 1937. Web. 14 Apr 2021.

Vancouver:

McDaniel LE. A study of certain Salmonella and similar bacteria from the intestine of the chicken. [Internet] [Thesis]. Kansas State University; 1937. [cited 2021 Apr 14]. Available from: http://hdl.handle.net/2097/18057.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

McDaniel LE. A study of certain Salmonella and similar bacteria from the intestine of the chicken. [Thesis]. Kansas State University; 1937. Available from: http://hdl.handle.net/2097/18057

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

12. Lunak, Zachary Ryan. The Role and Regulation of Quinol Oxidase in Rhizobium Etli.

Degree: 2015, Marquette University

URL: https://epublications.marquette.edu/dissertations_mu/504

► Aerobic bacteria typically respire by delivering electrons to oxygen via cytochrome c oxidases. In addition, many bacteria can respire by transferring electrons to oxygen via… (more)

▼ Aerobic bacteria typically respire by delivering electrons to oxygen via cytochrome c oxidases. In addition, many bacteria can respire by transferring electrons to oxygen via quinol oxidases. Exactly why bacteria contain quinol oxidases when they already have cytochrome c oxidases is unclear. In particular, the Cyo quinol oxidase, encoded by cyoABCD, is widespread among the dominant bacterial groups but its specific role is unknown. Rhizobium etli CFN42 provides an aerobic respiratory model in which Cyo can be directly compared to cytochrome c oxidases within the same organism. Mutants of the terminal oxidases in R. etli were constructed and their ability to grow in different physiological conditions was examined. The cyo mutant had noticable growth defects in low oxygen, low pH and low iron conditions. Furthermore, the cyo gene in the wild type was significantly up-regulated in these conditions. Conversely, in slow-growth conditions, such as stationary growth or growth in certain carbon sources, cyo was significantly down-regulated. A respiratory mutant, in which Cyo is the only viable terminal oxidase, had clear phenotypic defects in these conditions due to the regulation of cyo. Examination of the 5’ promoter region of cyo revealed multiple DNA binding sites for the transcription factor, ActR of the ActSR 2-component system. An actSR mutant was constructed and had significantly lower levels of cyo expression in all physiological conditions tested. The results suggest Cyo is important for growth and adaptation to low oxygen conditions, low pH, and low iron conditions in R. etli. Having an oxidase that enables the bacterium to aerobically respire in these conditions is of great benefit for bacteria, in particular soil bacteria that have to frequently adjust to adverse environmental conditions. Interestingly, each of these conditions would theoretically lead to higher quinol:quinone ratios in the cell. The activity of the transcriptional activator of cyo, ActSR, has been linked to the redox state of quinone in other bacteria, where quinone has been shown to inhibit ActSR activity. Altogether, the results indicate that the quinol:quinone ratio in the cell may be an important cue for Cyo utilization and expression. Advisors/Committee Members: Noel, Dale K., Hristova, Krassimira, Stuart, Rosemary.

Subjects/Keywords: Bacteriology; Microbiology

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APA (6^th Edition):

Lunak, Z. R. (2015). The Role and Regulation of Quinol Oxidase in Rhizobium Etli. (Thesis). Marquette University. Retrieved from https://epublications.marquette.edu/dissertations_mu/504

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lunak, Zachary Ryan. “The Role and Regulation of Quinol Oxidase in Rhizobium Etli.” 2015. Thesis, Marquette University. Accessed April 14, 2021. https://epublications.marquette.edu/dissertations_mu/504.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lunak, Zachary Ryan. “The Role and Regulation of Quinol Oxidase in Rhizobium Etli.” 2015. Web. 14 Apr 2021.

Vancouver:

Lunak ZR. The Role and Regulation of Quinol Oxidase in Rhizobium Etli. [Internet] [Thesis]. Marquette University; 2015. [cited 2021 Apr 14]. Available from: https://epublications.marquette.edu/dissertations_mu/504.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lunak ZR. The Role and Regulation of Quinol Oxidase in Rhizobium Etli. [Thesis]. Marquette University; 2015. Available from: https://epublications.marquette.edu/dissertations_mu/504

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

McGill University

13. Kahnke, M. Jeanne (Mary Jeanne). Staphylococcus nucleoproteins in connection with immunity.

Degree: PhD, Department of Bacteriology, 1949, McGill University

URL: https://escholarship.mcgill.ca/downloads/dr26z1353.pdf ; https://escholarship.mcgill.ca/concern/theses/0v838374r

► The minute size of the bacterial cell and the fact that the bacteria is unicellular has made the problem of liberating the bacterial nucleoproteins difficult,… (more)

Subjects/Keywords: Bacteriology.

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APA (6^th Edition):

Kahnke, M. J. (. J. (1949). Staphylococcus nucleoproteins in connection with immunity. (Doctoral Dissertation). McGill University. Retrieved from https://escholarship.mcgill.ca/downloads/dr26z1353.pdf ; https://escholarship.mcgill.ca/concern/theses/0v838374r

Chicago Manual of Style (16^th Edition):

Kahnke, M Jeanne (Mary Jeanne). “Staphylococcus nucleoproteins in connection with immunity.” 1949. Doctoral Dissertation, McGill University. Accessed April 14, 2021. https://escholarship.mcgill.ca/downloads/dr26z1353.pdf ; https://escholarship.mcgill.ca/concern/theses/0v838374r.

MLA Handbook (7^th Edition):

Kahnke, M Jeanne (Mary Jeanne). “Staphylococcus nucleoproteins in connection with immunity.” 1949. Web. 14 Apr 2021.

Vancouver:

Kahnke MJ(J. Staphylococcus nucleoproteins in connection with immunity. [Internet] [Doctoral dissertation]. McGill University; 1949. [cited 2021 Apr 14]. Available from: https://escholarship.mcgill.ca/downloads/dr26z1353.pdf ; https://escholarship.mcgill.ca/concern/theses/0v838374r.

Council of Science Editors:

Kahnke MJ(J. Staphylococcus nucleoproteins in connection with immunity. [Doctoral Dissertation]. McGill University; 1949. Available from: https://escholarship.mcgill.ca/downloads/dr26z1353.pdf ; https://escholarship.mcgill.ca/concern/theses/0v838374r

University of Louisville

14. Welsh, Blanche Mae. A comparison of various sizes of inverted vials used in Durham fermentation tubes.

Degree: MS, Department of Bacteriology, 1940, University of Louisville

URL: 10.18297/etd/1941 ; https://ir.library.louisville.edu/etd/1941

Subjects/Keywords: Bacteriology

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APA (6^th Edition):

Welsh, B. M. (1940). A comparison of various sizes of inverted vials used in Durham fermentation tubes. (Masters Thesis). University of Louisville. Retrieved from 10.18297/etd/1941 ; https://ir.library.louisville.edu/etd/1941

Chicago Manual of Style (16^th Edition):

Welsh, Blanche Mae. “A comparison of various sizes of inverted vials used in Durham fermentation tubes.” 1940. Masters Thesis, University of Louisville. Accessed April 14, 2021. 10.18297/etd/1941 ; https://ir.library.louisville.edu/etd/1941.

MLA Handbook (7^th Edition):

Welsh, Blanche Mae. “A comparison of various sizes of inverted vials used in Durham fermentation tubes.” 1940. Web. 14 Apr 2021.

Vancouver:

Welsh BM. A comparison of various sizes of inverted vials used in Durham fermentation tubes. [Internet] [Masters thesis]. University of Louisville; 1940. [cited 2021 Apr 14]. Available from: 10.18297/etd/1941 ; https://ir.library.louisville.edu/etd/1941.

Council of Science Editors:

Welsh BM. A comparison of various sizes of inverted vials used in Durham fermentation tubes. [Masters Thesis]. University of Louisville; 1940. Available from: 10.18297/etd/1941 ; https://ir.library.louisville.edu/etd/1941

Loyola University Chicago

15. Burke, Alice J. The Influence of Chemicals on the Growth of Bacteria.

Degree: MS, Bacteriology, 1932, Loyola University Chicago

URL: https://ecommons.luc.edu/luc_theses/13

Subjects/Keywords: Bacteriology

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APA (6^th Edition):

Burke, A. J. (1932). The Influence of Chemicals on the Growth of Bacteria. (Thesis). Loyola University Chicago. Retrieved from https://ecommons.luc.edu/luc_theses/13

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Burke, Alice J. “The Influence of Chemicals on the Growth of Bacteria.” 1932. Thesis, Loyola University Chicago. Accessed April 14, 2021. https://ecommons.luc.edu/luc_theses/13.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Burke, Alice J. “The Influence of Chemicals on the Growth of Bacteria.” 1932. Web. 14 Apr 2021.

Vancouver:

Burke AJ. The Influence of Chemicals on the Growth of Bacteria. [Internet] [Thesis]. Loyola University Chicago; 1932. [cited 2021 Apr 14]. Available from: https://ecommons.luc.edu/luc_theses/13.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Burke AJ. The Influence of Chemicals on the Growth of Bacteria. [Thesis]. Loyola University Chicago; 1932. Available from: https://ecommons.luc.edu/luc_theses/13

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Utah State University

16. Green, Kenneth Maxwell. Investigations into the Microbial Ecology and Limnology of Hyrum Reservoir, in Northern Utah.

Degree: MS, Animal, Dairy, and Veterinary Sciences, 1971, Utah State University

URL: https://digitalcommons.usu.edu/etd/3775

► A series of preliminary investigations was carried out to determine the factors promoting the dense, late summer waterblooms of Aphanizomenon flos-aguae in Hyrum Reservoir… (more)

▼ A series of preliminary investigations was carried out to determine the factors promoting the dense, late summer waterblooms of Aphanizomenon flos-aguae in Hyrum Reservoir in northern Utah. Attempts were made to culture the Aphanizomenon in the ASM-8a medium of O'Flaherty and Phinney (J. Phycol. 6:95-97. 1970), but no growth was obtained and the algae soon lysed. Cultures were maintained, without growth, for more than four months in a lake water – lake sediment medium at 17 C under 1500 lux flourescent light. Dissolved oxygen measurements using an in situ probe revealed the development of a sharp oxycline during the summer months; on one day the dissolved oxygen concentration was observed to drop from 118% saturation (8.6 mg O₂/1) at 6.5 meters depth, to 33% saturation (2.4 mg O₂/l) at 7 meters, with a concurrent temperature decrease from 21.5 C to 21 C. During the same period, pH was found to drop from 8.6 at the surface to 8.5 at 5 meters, 8.0 at 10 meters, and to 7.8 at the bottom (16 meters). Secchi disc depth corresponded to 14% of the incident radiation, this depth varying from l.3 to l.8 meters during the algal bloom. The photic zone (1% of incident radiation) extended to 3.5 meters depth. Water samples were collected from late April until early October , and these were analyzed for total organic carbon (TOC) using a Beckman model 915 total organic carbon analyzer. The organic carbon concentrations were found not to vary significantly with season or location. Many of the samples contained large numbers of Aphanizomenon but carbon analyses did not reflect this. It was concluded that the phytoplankton carbon in the reservoir was so much smaller than the carbon in the form of other organic materials, such as microseston, bacteria, detritus, colloids, and dissolved material, that fluctuations in algal carbon were therefore masked by the large amount of carbon continually present in these other forms. The mean organic carbon concentration for the lake was 4.6 mg/1 (n=118, s=1.47) and the range was from 1.2 to 8.9 mg TOC/1. The repeatability of measurements with the carbon analyzer is only within a range of 2 mg C/1, so the instrument is not sufficiently accurate for lake water analysis without the use of concentration techniques. Some trends were observed, but only at a l ow level of statistical significance: TOC concentration decreased slightly with depth, and TOC was correlated with stream discharge in the Little Bear River, which feeds the reservoir. An increase in TOC concentration in the reservoir during the algal bloom could not be verified statistically. Advisors/Committee Members: Frederick J. Post, ;.

Subjects/Keywords: Bacteriology

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APA (6^th Edition):

Green, K. M. (1971). Investigations into the Microbial Ecology and Limnology of Hyrum Reservoir, in Northern Utah. (Masters Thesis). Utah State University. Retrieved from https://digitalcommons.usu.edu/etd/3775

Chicago Manual of Style (16^th Edition):

Green, Kenneth Maxwell. “Investigations into the Microbial Ecology and Limnology of Hyrum Reservoir, in Northern Utah.” 1971. Masters Thesis, Utah State University. Accessed April 14, 2021. https://digitalcommons.usu.edu/etd/3775.

MLA Handbook (7^th Edition):

Green, Kenneth Maxwell. “Investigations into the Microbial Ecology and Limnology of Hyrum Reservoir, in Northern Utah.” 1971. Web. 14 Apr 2021.

Vancouver:

Green KM. Investigations into the Microbial Ecology and Limnology of Hyrum Reservoir, in Northern Utah. [Internet] [Masters thesis]. Utah State University; 1971. [cited 2021 Apr 14]. Available from: https://digitalcommons.usu.edu/etd/3775.

Council of Science Editors:

Green KM. Investigations into the Microbial Ecology and Limnology of Hyrum Reservoir, in Northern Utah. [Masters Thesis]. Utah State University; 1971. Available from: https://digitalcommons.usu.edu/etd/3775

Michigan State University

17. Edwards, Ogden Frazelle. A critique of the filtrability of bacteria.

Degree: MS, 1933, Michigan State University

URL: http://etd.lib.msu.edu/islandora/object/etd:10530

Subjects/Keywords: Bacteriology

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APA (6^th Edition):

Edwards, O. F. (1933). A critique of the filtrability of bacteria. (Masters Thesis). Michigan State University. Retrieved from http://etd.lib.msu.edu/islandora/object/etd:10530

Chicago Manual of Style (16^th Edition):

Edwards, Ogden Frazelle. “A critique of the filtrability of bacteria.” 1933. Masters Thesis, Michigan State University. Accessed April 14, 2021. http://etd.lib.msu.edu/islandora/object/etd:10530.

MLA Handbook (7^th Edition):

Edwards, Ogden Frazelle. “A critique of the filtrability of bacteria.” 1933. Web. 14 Apr 2021.

Vancouver:

Edwards OF. A critique of the filtrability of bacteria. [Internet] [Masters thesis]. Michigan State University; 1933. [cited 2021 Apr 14]. Available from: http://etd.lib.msu.edu/islandora/object/etd:10530.

Council of Science Editors:

Edwards OF. A critique of the filtrability of bacteria. [Masters Thesis]. Michigan State University; 1933. Available from: http://etd.lib.msu.edu/islandora/object/etd:10530

McGill University

18. Conklin, Raymond Leroy. A Sporadic Outbreak in Cattle Resembling Tetanus.

Degree: MS, Department of Bacteriology, 1923, McGill University

URL: https://escholarship.mcgill.ca/downloads/70795b43x.pdf ; https://escholarship.mcgill.ca/concern/theses/vd66w2397

► The disease has persisted and has been confused with other diseases of a contagious and infectious nature. The clinical symptoms have indicated forage, arsenic, and… (more)

Subjects/Keywords: Bacteriology.

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APA (6^th Edition):

Conklin, R. L. (1923). A Sporadic Outbreak in Cattle Resembling Tetanus. (Masters Thesis). McGill University. Retrieved from https://escholarship.mcgill.ca/downloads/70795b43x.pdf ; https://escholarship.mcgill.ca/concern/theses/vd66w2397

Chicago Manual of Style (16^th Edition):

Conklin, Raymond Leroy. “A Sporadic Outbreak in Cattle Resembling Tetanus.” 1923. Masters Thesis, McGill University. Accessed April 14, 2021. https://escholarship.mcgill.ca/downloads/70795b43x.pdf ; https://escholarship.mcgill.ca/concern/theses/vd66w2397.

MLA Handbook (7^th Edition):

Conklin, Raymond Leroy. “A Sporadic Outbreak in Cattle Resembling Tetanus.” 1923. Web. 14 Apr 2021.

Vancouver:

Conklin RL. A Sporadic Outbreak in Cattle Resembling Tetanus. [Internet] [Masters thesis]. McGill University; 1923. [cited 2021 Apr 14]. Available from: https://escholarship.mcgill.ca/downloads/70795b43x.pdf ; https://escholarship.mcgill.ca/concern/theses/vd66w2397.

Council of Science Editors:

Conklin RL. A Sporadic Outbreak in Cattle Resembling Tetanus. [Masters Thesis]. McGill University; 1923. Available from: https://escholarship.mcgill.ca/downloads/70795b43x.pdf ; https://escholarship.mcgill.ca/concern/theses/vd66w2397

McGill University

19. Shanly, Eleanor. On the heat resistance of bacterial spores, with a consideration of the nature of the spore-like bodies seen in B. tuberculosis and allied forms.

Degree: MS, Department of Bacteriology, 1914, McGill University

URL: https://escholarship.mcgill.ca/downloads/zp38wg382.pdf ; https://escholarship.mcgill.ca/concern/theses/3j333508p

► Every bacteriologist will agree that the proof of the spore lies in its resistance. There is abundant evidence on every hand that its resistance to… (more)

Subjects/Keywords: Bacteriology.

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APA (6^th Edition):

Shanly, E. (1914). On the heat resistance of bacterial spores, with a consideration of the nature of the spore-like bodies seen in B. tuberculosis and allied forms. (Masters Thesis). McGill University. Retrieved from https://escholarship.mcgill.ca/downloads/zp38wg382.pdf ; https://escholarship.mcgill.ca/concern/theses/3j333508p

Chicago Manual of Style (16^th Edition):

Shanly, Eleanor. “On the heat resistance of bacterial spores, with a consideration of the nature of the spore-like bodies seen in B. tuberculosis and allied forms.” 1914. Masters Thesis, McGill University. Accessed April 14, 2021. https://escholarship.mcgill.ca/downloads/zp38wg382.pdf ; https://escholarship.mcgill.ca/concern/theses/3j333508p.

MLA Handbook (7^th Edition):

Shanly, Eleanor. “On the heat resistance of bacterial spores, with a consideration of the nature of the spore-like bodies seen in B. tuberculosis and allied forms.” 1914. Web. 14 Apr 2021.

Vancouver:

Shanly E. On the heat resistance of bacterial spores, with a consideration of the nature of the spore-like bodies seen in B. tuberculosis and allied forms. [Internet] [Masters thesis]. McGill University; 1914. [cited 2021 Apr 14]. Available from: https://escholarship.mcgill.ca/downloads/zp38wg382.pdf ; https://escholarship.mcgill.ca/concern/theses/3j333508p.

Council of Science Editors:

Shanly E. On the heat resistance of bacterial spores, with a consideration of the nature of the spore-like bodies seen in B. tuberculosis and allied forms. [Masters Thesis]. McGill University; 1914. Available from: https://escholarship.mcgill.ca/downloads/zp38wg382.pdf ; https://escholarship.mcgill.ca/concern/theses/3j333508p

McGill University

20. Newton, Dorothy E. Marine spore forming bacteria.

Degree: MS, Department of Bacteriology, 1922, McGill University

URL: https://escholarship.mcgill.ca/downloads/bc386n217.pdf ; https://escholarship.mcgill.ca/concern/theses/37720g58w

This investigation was undertaken with the object of isolating and studying the spore forming bacteria found in the sea, in fish and shell-fish such as herring, clam, lobster, etc. [...] Advisors/Committee Members: Dickson, B. & Harrison, F. (Supervisors).

Subjects/Keywords: Bacteriology.

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APA (6^th Edition):

Newton, D. E. (1922). Marine spore forming bacteria. (Masters Thesis). McGill University. Retrieved from https://escholarship.mcgill.ca/downloads/bc386n217.pdf ; https://escholarship.mcgill.ca/concern/theses/37720g58w

Chicago Manual of Style (16^th Edition):

Newton, Dorothy E. “Marine spore forming bacteria.” 1922. Masters Thesis, McGill University. Accessed April 14, 2021. https://escholarship.mcgill.ca/downloads/bc386n217.pdf ; https://escholarship.mcgill.ca/concern/theses/37720g58w.

MLA Handbook (7^th Edition):

Newton, Dorothy E. “Marine spore forming bacteria.” 1922. Web. 14 Apr 2021.

Vancouver:

Newton DE. Marine spore forming bacteria. [Internet] [Masters thesis]. McGill University; 1922. [cited 2021 Apr 14]. Available from: https://escholarship.mcgill.ca/downloads/bc386n217.pdf ; https://escholarship.mcgill.ca/concern/theses/37720g58w.

Council of Science Editors:

Newton DE. Marine spore forming bacteria. [Masters Thesis]. McGill University; 1922. Available from: https://escholarship.mcgill.ca/downloads/bc386n217.pdf ; https://escholarship.mcgill.ca/concern/theses/37720g58w

McGill University

21. McMaster, Norman Berry. The microbiology of air-dried cultivated soils.

Degree: MS, Department of Bacteriology, 1932, McGill University

URL: https://escholarship.mcgill.ca/downloads/q237hv49b.pdf ; https://escholarship.mcgill.ca/concern/theses/vd66w260g

► The beneficial effect of the drying of soils upon their fertility has been a recognised fact in agricultural practice for many centuries. Russell, (16., p.p.… (more)

Subjects/Keywords: Bacteriology.

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APA (6^th Edition):

McMaster, N. B. (1932). The microbiology of air-dried cultivated soils. (Masters Thesis). McGill University. Retrieved from https://escholarship.mcgill.ca/downloads/q237hv49b.pdf ; https://escholarship.mcgill.ca/concern/theses/vd66w260g

Chicago Manual of Style (16^th Edition):

McMaster, Norman Berry. “The microbiology of air-dried cultivated soils.” 1932. Masters Thesis, McGill University. Accessed April 14, 2021. https://escholarship.mcgill.ca/downloads/q237hv49b.pdf ; https://escholarship.mcgill.ca/concern/theses/vd66w260g.

MLA Handbook (7^th Edition):

McMaster, Norman Berry. “The microbiology of air-dried cultivated soils.” 1932. Web. 14 Apr 2021.

Vancouver:

McMaster NB. The microbiology of air-dried cultivated soils. [Internet] [Masters thesis]. McGill University; 1932. [cited 2021 Apr 14]. Available from: https://escholarship.mcgill.ca/downloads/q237hv49b.pdf ; https://escholarship.mcgill.ca/concern/theses/vd66w260g.

Council of Science Editors:

McMaster NB. The microbiology of air-dried cultivated soils. [Masters Thesis]. McGill University; 1932. Available from: https://escholarship.mcgill.ca/downloads/q237hv49b.pdf ; https://escholarship.mcgill.ca/concern/theses/vd66w260g

McGill University

22. Kennedy, Margaret E. The red discoloration of dried codfish.

Degree: MS, Department of Bacteriology, 1921, McGill University

URL: https://escholarship.mcgill.ca/downloads/9019s521d.pdf ; https://escholarship.mcgill.ca/concern/theses/tm70mz28c

The codfish Industry provides for the Dominion of Canada a revenue of no inconsiderable amount - according to government statistics, a revenue which surpasses that derived from all other fish enterprises, salmon alone excepted. [...] Advisors/Committee Members: Harrison, F. (Supervisor).

Subjects/Keywords: Bacteriology.

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APA (6^th Edition):

Kennedy, M. E. (1921). The red discoloration of dried codfish. (Masters Thesis). McGill University. Retrieved from https://escholarship.mcgill.ca/downloads/9019s521d.pdf ; https://escholarship.mcgill.ca/concern/theses/tm70mz28c

Chicago Manual of Style (16^th Edition):

Kennedy, Margaret E. “The red discoloration of dried codfish.” 1921. Masters Thesis, McGill University. Accessed April 14, 2021. https://escholarship.mcgill.ca/downloads/9019s521d.pdf ; https://escholarship.mcgill.ca/concern/theses/tm70mz28c.

MLA Handbook (7^th Edition):

Kennedy, Margaret E. “The red discoloration of dried codfish.” 1921. Web. 14 Apr 2021.

Vancouver:

Kennedy ME. The red discoloration of dried codfish. [Internet] [Masters thesis]. McGill University; 1921. [cited 2021 Apr 14]. Available from: https://escholarship.mcgill.ca/downloads/9019s521d.pdf ; https://escholarship.mcgill.ca/concern/theses/tm70mz28c.

Council of Science Editors:

Kennedy ME. The red discoloration of dried codfish. [Masters Thesis]. McGill University; 1921. Available from: https://escholarship.mcgill.ca/downloads/9019s521d.pdf ; https://escholarship.mcgill.ca/concern/theses/tm70mz28c

McGill University

23. Whitehead, Howard. A. The Lethal and Mutagenic Effects of Ultraviolet Light on Escherichia Coli.

Degree: MS, Department of Biology, 1951, McGill University

URL: https://escholarship.mcgill.ca/downloads/2z10wt13m.pdf ; https://escholarship.mcgill.ca/concern/theses/m326m4543

► It would be hard to name a papaer or date which might be regarded as the starting point for the study of the effects of… (more)

Subjects/Keywords: Bacteriology.

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APA (6^th Edition):

Whitehead, H. A. (1951). The Lethal and Mutagenic Effects of Ultraviolet Light on Escherichia Coli. (Masters Thesis). McGill University. Retrieved from https://escholarship.mcgill.ca/downloads/2z10wt13m.pdf ; https://escholarship.mcgill.ca/concern/theses/m326m4543

Chicago Manual of Style (16^th Edition):

Whitehead, Howard A. “The Lethal and Mutagenic Effects of Ultraviolet Light on Escherichia Coli.” 1951. Masters Thesis, McGill University. Accessed April 14, 2021. https://escholarship.mcgill.ca/downloads/2z10wt13m.pdf ; https://escholarship.mcgill.ca/concern/theses/m326m4543.

MLA Handbook (7^th Edition):

Whitehead, Howard A. “The Lethal and Mutagenic Effects of Ultraviolet Light on Escherichia Coli.” 1951. Web. 14 Apr 2021.

Vancouver:

Whitehead HA. The Lethal and Mutagenic Effects of Ultraviolet Light on Escherichia Coli. [Internet] [Masters thesis]. McGill University; 1951. [cited 2021 Apr 14]. Available from: https://escholarship.mcgill.ca/downloads/2z10wt13m.pdf ; https://escholarship.mcgill.ca/concern/theses/m326m4543.

Council of Science Editors:

Whitehead HA. The Lethal and Mutagenic Effects of Ultraviolet Light on Escherichia Coli. [Masters Thesis]. McGill University; 1951. Available from: https://escholarship.mcgill.ca/downloads/2z10wt13m.pdf ; https://escholarship.mcgill.ca/concern/theses/m326m4543

McGill University

24. Symons, Jennie L. Organisms which cause blackening in clams.

Degree: MS, Department of Bacteriology, 1921, McGill University

URL: https://escholarship.mcgill.ca/downloads/w37639664.pdf ; https://escholarship.mcgill.ca/concern/theses/p2676z51q

► The problem of blackening is a serious one In the canning industry. Blackening is a common form of deterioration in advanced stages of which the… (more)

Subjects/Keywords: Bacteriology.

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Symons, J. L. (1921). Organisms which cause blackening in clams. (Masters Thesis). McGill University. Retrieved from https://escholarship.mcgill.ca/downloads/w37639664.pdf ; https://escholarship.mcgill.ca/concern/theses/p2676z51q

Chicago Manual of Style (16^th Edition):

Symons, Jennie L. “Organisms which cause blackening in clams.” 1921. Masters Thesis, McGill University. Accessed April 14, 2021. https://escholarship.mcgill.ca/downloads/w37639664.pdf ; https://escholarship.mcgill.ca/concern/theses/p2676z51q.

MLA Handbook (7^th Edition):

Symons, Jennie L. “Organisms which cause blackening in clams.” 1921. Web. 14 Apr 2021.

Vancouver:

Symons JL. Organisms which cause blackening in clams. [Internet] [Masters thesis]. McGill University; 1921. [cited 2021 Apr 14]. Available from: https://escholarship.mcgill.ca/downloads/w37639664.pdf ; https://escholarship.mcgill.ca/concern/theses/p2676z51q.

Council of Science Editors:

Symons JL. Organisms which cause blackening in clams. [Masters Thesis]. McGill University; 1921. Available from: https://escholarship.mcgill.ca/downloads/w37639664.pdf ; https://escholarship.mcgill.ca/concern/theses/p2676z51q

McGill University

25. Bynoe, Evan Theodore. A systematic study of the Genus Mycobacterium.

Degree: MS, Department of Bacteriology, 1931, McGill University

URL: https://escholarship.mcgill.ca/downloads/02870z940.pdf ; https://escholarship.mcgill.ca/concern/theses/qb98mj278

► Lehmann and Neumann (54) first proposed the generic name Mycobacteriumn for the group of acid-fast bacteria, with Mycobacterium tuberculosis - the tubercle bacillus of Koch… (more)

Subjects/Keywords: Bacteriology.

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APA (6^th Edition):

Bynoe, E. T. (1931). A systematic study of the Genus Mycobacterium. (Masters Thesis). McGill University. Retrieved from https://escholarship.mcgill.ca/downloads/02870z940.pdf ; https://escholarship.mcgill.ca/concern/theses/qb98mj278

Chicago Manual of Style (16^th Edition):

Bynoe, Evan Theodore. “A systematic study of the Genus Mycobacterium.” 1931. Masters Thesis, McGill University. Accessed April 14, 2021. https://escholarship.mcgill.ca/downloads/02870z940.pdf ; https://escholarship.mcgill.ca/concern/theses/qb98mj278.

MLA Handbook (7^th Edition):

Bynoe, Evan Theodore. “A systematic study of the Genus Mycobacterium.” 1931. Web. 14 Apr 2021.

Vancouver:

Bynoe ET. A systematic study of the Genus Mycobacterium. [Internet] [Masters thesis]. McGill University; 1931. [cited 2021 Apr 14]. Available from: https://escholarship.mcgill.ca/downloads/02870z940.pdf ; https://escholarship.mcgill.ca/concern/theses/qb98mj278.

Council of Science Editors:

Bynoe ET. A systematic study of the Genus Mycobacterium. [Masters Thesis]. McGill University; 1931. Available from: https://escholarship.mcgill.ca/downloads/02870z940.pdf ; https://escholarship.mcgill.ca/concern/theses/qb98mj278

McGill University

26. Sadler, Wilfrid. Studies in marine bacteriology : Bacterial destruction of Copepods ; The bacteriology of swelled canned fish.

Degree: MS, Department of Bacteriology, 1917, McGill University

URL: https://escholarship.mcgill.ca/downloads/1z40kw515.pdf ; https://escholarship.mcgill.ca/concern/theses/pc289m65v

► At the request and on the suggestion of Dr. Willey l have proceeded with the examination of the cultures secured and have obtained in pure… (more)

Subjects/Keywords: Bacteriology.

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sadler, W. (1917). Studies in marine bacteriology : Bacterial destruction of Copepods ; The bacteriology of swelled canned fish. (Masters Thesis). McGill University. Retrieved from https://escholarship.mcgill.ca/downloads/1z40kw515.pdf ; https://escholarship.mcgill.ca/concern/theses/pc289m65v

Chicago Manual of Style (16^th Edition):

Sadler, Wilfrid. “Studies in marine bacteriology : Bacterial destruction of Copepods ; The bacteriology of swelled canned fish.” 1917. Masters Thesis, McGill University. Accessed April 14, 2021. https://escholarship.mcgill.ca/downloads/1z40kw515.pdf ; https://escholarship.mcgill.ca/concern/theses/pc289m65v.

MLA Handbook (7^th Edition):

Sadler, Wilfrid. “Studies in marine bacteriology : Bacterial destruction of Copepods ; The bacteriology of swelled canned fish.” 1917. Web. 14 Apr 2021.

Vancouver:

Sadler W. Studies in marine bacteriology : Bacterial destruction of Copepods ; The bacteriology of swelled canned fish. [Internet] [Masters thesis]. McGill University; 1917. [cited 2021 Apr 14]. Available from: https://escholarship.mcgill.ca/downloads/1z40kw515.pdf ; https://escholarship.mcgill.ca/concern/theses/pc289m65v.

Council of Science Editors:

Sadler W. Studies in marine bacteriology : Bacterial destruction of Copepods ; The bacteriology of swelled canned fish. [Masters Thesis]. McGill University; 1917. Available from: https://escholarship.mcgill.ca/downloads/1z40kw515.pdf ; https://escholarship.mcgill.ca/concern/theses/pc289m65v

McGill University

27. Anderson, Marian. On the relative rate of dextrose fermentation of generations of colon bacteria grown on sugar-free media.

Degree: MS, Department of Bacteriology, 1920, McGill University

URL: https://escholarship.mcgill.ca/downloads/pv63g2785.pdf ; https://escholarship.mcgill.ca/concern/theses/f7623g24t

► Ever since the colon bacillus was isolated by Escherich in 1856 from the dejecta of a breast-fed infant and subsequently, in 1884, from the feces… (more)

Subjects/Keywords: Bacteriology.

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APA (6^th Edition):

Anderson, M. (1920). On the relative rate of dextrose fermentation of generations of colon bacteria grown on sugar-free media. (Masters Thesis). McGill University. Retrieved from https://escholarship.mcgill.ca/downloads/pv63g2785.pdf ; https://escholarship.mcgill.ca/concern/theses/f7623g24t

Chicago Manual of Style (16^th Edition):

Anderson, Marian. “On the relative rate of dextrose fermentation of generations of colon bacteria grown on sugar-free media.” 1920. Masters Thesis, McGill University. Accessed April 14, 2021. https://escholarship.mcgill.ca/downloads/pv63g2785.pdf ; https://escholarship.mcgill.ca/concern/theses/f7623g24t.

MLA Handbook (7^th Edition):

Anderson, Marian. “On the relative rate of dextrose fermentation of generations of colon bacteria grown on sugar-free media.” 1920. Web. 14 Apr 2021.

Vancouver:

Anderson M. On the relative rate of dextrose fermentation of generations of colon bacteria grown on sugar-free media. [Internet] [Masters thesis]. McGill University; 1920. [cited 2021 Apr 14]. Available from: https://escholarship.mcgill.ca/downloads/pv63g2785.pdf ; https://escholarship.mcgill.ca/concern/theses/f7623g24t.

Council of Science Editors:

Anderson M. On the relative rate of dextrose fermentation of generations of colon bacteria grown on sugar-free media. [Masters Thesis]. McGill University; 1920. Available from: https://escholarship.mcgill.ca/downloads/pv63g2785.pdf ; https://escholarship.mcgill.ca/concern/theses/f7623g24t

McGill University

28. Byrne, Joseph Lawrence. The differentiation of salmonella pullorum by fermentation and agglutination reactions.

Degree: MS, Department of Bacteriology, 1939, McGill University

URL: https://escholarship.mcgill.ca/downloads/rj430768s.pdf ; https://escholarship.mcgill.ca/concern/theses/3197xp74n

► Although Salmonella pullorum has for many years been the subject of much investigation resulting in the accumulation or considerable knowledge of its various characteristics, there… (more)

Subjects/Keywords: Bacteriology.

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APA (6^th Edition):

Byrne, J. L. (1939). The differentiation of salmonella pullorum by fermentation and agglutination reactions. (Masters Thesis). McGill University. Retrieved from https://escholarship.mcgill.ca/downloads/rj430768s.pdf ; https://escholarship.mcgill.ca/concern/theses/3197xp74n

Chicago Manual of Style (16^th Edition):

Byrne, Joseph Lawrence. “The differentiation of salmonella pullorum by fermentation and agglutination reactions.” 1939. Masters Thesis, McGill University. Accessed April 14, 2021. https://escholarship.mcgill.ca/downloads/rj430768s.pdf ; https://escholarship.mcgill.ca/concern/theses/3197xp74n.

MLA Handbook (7^th Edition):

Byrne, Joseph Lawrence. “The differentiation of salmonella pullorum by fermentation and agglutination reactions.” 1939. Web. 14 Apr 2021.

Vancouver:

Byrne JL. The differentiation of salmonella pullorum by fermentation and agglutination reactions. [Internet] [Masters thesis]. McGill University; 1939. [cited 2021 Apr 14]. Available from: https://escholarship.mcgill.ca/downloads/rj430768s.pdf ; https://escholarship.mcgill.ca/concern/theses/3197xp74n.

Council of Science Editors:

Byrne JL. The differentiation of salmonella pullorum by fermentation and agglutination reactions. [Masters Thesis]. McGill University; 1939. Available from: https://escholarship.mcgill.ca/downloads/rj430768s.pdf ; https://escholarship.mcgill.ca/concern/theses/3197xp74n

McMaster University

29. Puri, Akshita. CULTURING AIRWAY POLYMICROBIAL COMMUNITIES UNDER CONTINUOUS FLOW CONDITIONS.

Degree: MSc, 2013, McMaster University

URL: http://hdl.handle.net/11375/13288

►

Microbes are ubiquitous in the biosphere and play important roles in natural ecosystems. They are typically present as diverse, complex communities, and in humans… (more)

▼

Microbes are ubiquitous in the biosphere and play important roles in natural ecosystems. They are typically present as diverse, complex communities, and in humans these communities are present on all exposed surfaces and mucosal tissues. The human upper respiratory tract harbors a complex microbiome and the composition includes what are traditionally considered commensal organisms, including a significant proportion of anaerobic bacteria. It is generally assumed that most of the bacteria from any particular environment cannot be readily cultivated, including the human microbiome. Some in vitro microfluidic and in vivo models are available to study the airway microbial communities, however these methods are expensive, limited and are not practical for experiments manipulating the community. A robust culture-based approach that can propagate these polymicrobial communities has been developed in this study to investigate spatial-temporal changes in bacterial populations in vitro. Matrix embedded synthetic bacterial communities, comprised of aerobes and anaerobes, were cultivated in continuous flow cell systems. The structure of communities propagated in these systems was compared to those in static and shaken batch cultures. The data shows that reproducible stable bacterial communities can be propagated with these culture methods, however the community composition varies considerably with the approach used. Only matrix embedded communities, cultured under continuous flow conditions, could successfully retain obligate anaerobes when flow cell systems were operated in an aerobic environment. This optimized method was used for culturing complex and diverse natural communities from clinical samples (sputum). The majority of bacteria present in the original sample were recovered in flow cell cultures and the methodology was consistent. This study provides an experimental system that can be used for examining microbial community dynamics and community structure-function relationship.

Master of Science (MSc)

Advisors/Committee Members: Surette, Michael G., Biochemistry and Biomedical Sciences.

Subjects/Keywords: Bacteriology; Environmental Microbiology and Microbial Ecology; Bacteriology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Puri, A. (2013). CULTURING AIRWAY POLYMICROBIAL COMMUNITIES UNDER CONTINUOUS FLOW CONDITIONS. (Masters Thesis). McMaster University. Retrieved from http://hdl.handle.net/11375/13288

Chicago Manual of Style (16^th Edition):

Puri, Akshita. “CULTURING AIRWAY POLYMICROBIAL COMMUNITIES UNDER CONTINUOUS FLOW CONDITIONS.” 2013. Masters Thesis, McMaster University. Accessed April 14, 2021. http://hdl.handle.net/11375/13288.

MLA Handbook (7^th Edition):

Puri, Akshita. “CULTURING AIRWAY POLYMICROBIAL COMMUNITIES UNDER CONTINUOUS FLOW CONDITIONS.” 2013. Web. 14 Apr 2021.

Vancouver:

Puri A. CULTURING AIRWAY POLYMICROBIAL COMMUNITIES UNDER CONTINUOUS FLOW CONDITIONS. [Internet] [Masters thesis]. McMaster University; 2013. [cited 2021 Apr 14]. Available from: http://hdl.handle.net/11375/13288.

Council of Science Editors:

Puri A. CULTURING AIRWAY POLYMICROBIAL COMMUNITIES UNDER CONTINUOUS FLOW CONDITIONS. [Masters Thesis]. McMaster University; 2013. Available from: http://hdl.handle.net/11375/13288

Iowa State University

30. Thompson, Nancy Elizabeth. Role of bacteriophage in the acquisition of competence for genetic transformation and transfection in Staphylococcus aureus and related studies.

Degree: 1979, Iowa State University

URL: https://lib.dr.iastate.edu/rtd/7255

Subjects/Keywords: Bacteriology; Microbiology; Bacteriology

Record Details Similar Records Cite « Share

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Thompson, N. E. (1979). Role of bacteriophage in the acquisition of competence for genetic transformation and transfection in Staphylococcus aureus and related studies. (Thesis). Iowa State University. Retrieved from https://lib.dr.iastate.edu/rtd/7255

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Thompson, Nancy Elizabeth. “Role of bacteriophage in the acquisition of competence for genetic transformation and transfection in Staphylococcus aureus and related studies.” 1979. Thesis, Iowa State University. Accessed April 14, 2021. https://lib.dr.iastate.edu/rtd/7255.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Thompson, Nancy Elizabeth. “Role of bacteriophage in the acquisition of competence for genetic transformation and transfection in Staphylococcus aureus and related studies.” 1979. Web. 14 Apr 2021.

Vancouver:

Thompson NE. Role of bacteriophage in the acquisition of competence for genetic transformation and transfection in Staphylococcus aureus and related studies. [Internet] [Thesis]. Iowa State University; 1979. [cited 2021 Apr 14]. Available from: https://lib.dr.iastate.edu/rtd/7255.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Thompson NE. Role of bacteriophage in the acquisition of competence for genetic transformation and transfection in Staphylococcus aureus and related studies. [Thesis]. Iowa State University; 1979. Available from: https://lib.dr.iastate.edu/rtd/7255

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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Open Access Theses and Dissertations