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You searched for subject:(asialofetuin). Showing records 1 – 3 of 3 total matches.

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University of Alberta

1. Kharaud, Gagandeep. Asialofetuin-coated PLGA Nanoparticles for Targeting Hepatocytes.

Degree: Masters of Science in Pharmaceutical Sciences, Faculty of Pharmacy and Pharmaceutical Sciences, 2010, University of Alberta

The purpose of this project was to formulate, modify and evaluate nanoparticles for targeting asialoglycoprotein receptor found in large numbers exclusively on hepatocyte surfaces. Our goal is to increase the efficacy, reduce the side effects and the cost of the hydrophilic drugs administered to cure diseases like hepatitis C virus. Selective drug delivery into targeted cells using nanoparticles is one effective approach to enhance activity and avoid systemic side effects. Here we describe our effort towards the development of specially engineered poly(D,L-lactic-co-glycolic acid) nanoparticles using double emulsion method and surface coat them with asialofetuin for targeted delivery into hepatocytes. Bovine Serum Albumin-coated nanoparticles were prepared as a negative control. Furthermore, covalently conjugated protein on nanoparticle was labeled with rhodamine and was used for cell-based studies. Results from these studies indicated that asialofetuin conjugated with nanoparticles showed enhanced and selective uptake by hepatocytes compared to nanoparticles conjugated with Bovine Serum Albumin.

Subjects/Keywords: asialofetuin; hepatocytes; nanoparticles

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Kharaud, G. (2010). Asialofetuin-coated PLGA Nanoparticles for Targeting Hepatocytes. (Masters Thesis). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/2f75r9133

Chicago Manual of Style (16th Edition):

Kharaud, Gagandeep. “Asialofetuin-coated PLGA Nanoparticles for Targeting Hepatocytes.” 2010. Masters Thesis, University of Alberta. Accessed February 26, 2021. https://era.library.ualberta.ca/files/2f75r9133.

MLA Handbook (7th Edition):

Kharaud, Gagandeep. “Asialofetuin-coated PLGA Nanoparticles for Targeting Hepatocytes.” 2010. Web. 26 Feb 2021.

Vancouver:

Kharaud G. Asialofetuin-coated PLGA Nanoparticles for Targeting Hepatocytes. [Internet] [Masters thesis]. University of Alberta; 2010. [cited 2021 Feb 26]. Available from: https://era.library.ualberta.ca/files/2f75r9133.

Council of Science Editors:

Kharaud G. Asialofetuin-coated PLGA Nanoparticles for Targeting Hepatocytes. [Masters Thesis]. University of Alberta; 2010. Available from: https://era.library.ualberta.ca/files/2f75r9133


Penn State University

2. Blome, Matthew. CHARACTERIZATION OF RICIN BINDING TO MULTIVALENT BOVINE SERUM ALBUMIN-BASED NEOGLYCOCONJUGATES .

Degree: 2008, Penn State University

Ricin toxin is a potent type 2 ribosome inactivating protein (RIP) from the plant, Ricinus communis. It has the potential to be used as a bioterrorism agent due to its high toxicity and the relative ease with which it can be obtained/purified. Ricin is a heterodimeric protein consisting of 2 subunits. The A-chain (RTA) is an N-glycosidase that halts protein synthesis by catalyzing the depurination of a specific adenine residue on ribosomal RNA. RTA is linked to the B-chain (RTB) via a single disulphide bridge. RTB is a lectin, containing 2 galactose binding sites ~70Å apart, that plays an essential role in mediating the binding and internalization of the toxin. While the binding of ricin to galactose has been studied, characterization of the binding of ricin to more complex carbohydrate ligands (i.e., glycoproteins and glycolipids) that might be present on the surface of a cell is lacking. In contrast to the low affinity of RTB for monovalent galactose (reported to be between 0.28 and 3.5x 10-4M), its affinity for asialofetuin (ASF) is about 1,000 times greater than it is to monovalent galactose. This observation supports the hypothesis that ricin exhibits multivalency when it adheres to ASF. The goal of this work was to synthesize multivalent carbohydrate ligands, use them to determine whether ricin would bind to them in a multivalent fashion, and to identify general saccharide characteristics that affect binding. To account for the distance between the two galactose binding sites on RTB, bovine serum albumin (BSA) was selected as the oligosaccharide carrier. BSA-based neoglycoconjugates were synthesized and their efficacy as ligands for the toxin monitored using surface plasmon resonance (SPR). The results indicated that ricin did exhibit multivalency when it interacted with appropriately derivatized BSA neoglycoconjugates and that it appeared to bind more efficiently to specific longer chain oligosaccharide ligands than to disaccharides. These observations have important implications for the development of possible antidotes for the treatment of people inadvertently exposed to ricin, for the use of RTB in drug deliver, and for optimization of ricin biosensors. Advisors/Committee Members: Cara Lynne Schengrund, Committee Chair/Co-Chair, Ira Joseph Ropson, Committee Member, Thomas E Spratt, Committee Member, Mark Kester, Committee Member, Michael Pishko, Committee Member.

Subjects/Keywords: glycoconjugate; asialofetuin; surface plasmon resonance; asialo-GM1; ricin; bovine serum albumin; multivalent

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Blome, M. (2008). CHARACTERIZATION OF RICIN BINDING TO MULTIVALENT BOVINE SERUM ALBUMIN-BASED NEOGLYCOCONJUGATES . (Thesis). Penn State University. Retrieved from https://submit-etda.libraries.psu.edu/catalog/8208

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Blome, Matthew. “CHARACTERIZATION OF RICIN BINDING TO MULTIVALENT BOVINE SERUM ALBUMIN-BASED NEOGLYCOCONJUGATES .” 2008. Thesis, Penn State University. Accessed February 26, 2021. https://submit-etda.libraries.psu.edu/catalog/8208.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Blome, Matthew. “CHARACTERIZATION OF RICIN BINDING TO MULTIVALENT BOVINE SERUM ALBUMIN-BASED NEOGLYCOCONJUGATES .” 2008. Web. 26 Feb 2021.

Vancouver:

Blome M. CHARACTERIZATION OF RICIN BINDING TO MULTIVALENT BOVINE SERUM ALBUMIN-BASED NEOGLYCOCONJUGATES . [Internet] [Thesis]. Penn State University; 2008. [cited 2021 Feb 26]. Available from: https://submit-etda.libraries.psu.edu/catalog/8208.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Blome M. CHARACTERIZATION OF RICIN BINDING TO MULTIVALENT BOVINE SERUM ALBUMIN-BASED NEOGLYCOCONJUGATES . [Thesis]. Penn State University; 2008. Available from: https://submit-etda.libraries.psu.edu/catalog/8208

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

3. Καλλιντέρη, Παρασκευή. Λιποσώματα που ενσωματώνουν το ασιαλογλυκοπεπτίδιο A-F2 της φετουΐνης: μελέτη φυσικοχημικής σταθερότητας και ενδοκυττάρωσης σε ηπατοκύτταρα που φέρουν τον υποδοχέα της ασιαλογλυκοπρωτεΐνης.

Degree: 2000, Πανεπιστήμιο Πατρών; University of Patras

Subjects/Keywords: Ασιαλοφετουΐνη; Λιποσώματα; Ηπατοκύτταρα; Υποδοχέας ασιαλογλυκοπρωτεΐνης; Χολικά άλατα; Asialofetuin; Liposomes; Liver cells; Asialoglycoprotein receptor; Cholate salts

Record DetailsSimilar RecordsGoogle PlusoneFacebookTwitterCiteULikeMendeleyreddit

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Καλλιντέρη, . (2000). Λιποσώματα που ενσωματώνουν το ασιαλογλυκοπεπτίδιο A-F2 της φετουΐνης: μελέτη φυσικοχημικής σταθερότητας και ενδοκυττάρωσης σε ηπατοκύτταρα που φέρουν τον υποδοχέα της ασιαλογλυκοπρωτεΐνης. (Thesis). Πανεπιστήμιο Πατρών; University of Patras. Retrieved from http://hdl.handle.net/10442/hedi/12558

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Καλλιντέρη, Παρασκευή. “Λιποσώματα που ενσωματώνουν το ασιαλογλυκοπεπτίδιο A-F2 της φετουΐνης: μελέτη φυσικοχημικής σταθερότητας και ενδοκυττάρωσης σε ηπατοκύτταρα που φέρουν τον υποδοχέα της ασιαλογλυκοπρωτεΐνης.” 2000. Thesis, Πανεπιστήμιο Πατρών; University of Patras. Accessed February 26, 2021. http://hdl.handle.net/10442/hedi/12558.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Καλλιντέρη, Παρασκευή. “Λιποσώματα που ενσωματώνουν το ασιαλογλυκοπεπτίδιο A-F2 της φετουΐνης: μελέτη φυσικοχημικής σταθερότητας και ενδοκυττάρωσης σε ηπατοκύτταρα που φέρουν τον υποδοχέα της ασιαλογλυκοπρωτεΐνης.” 2000. Web. 26 Feb 2021.

Vancouver:

Καλλιντέρη . Λιποσώματα που ενσωματώνουν το ασιαλογλυκοπεπτίδιο A-F2 της φετουΐνης: μελέτη φυσικοχημικής σταθερότητας και ενδοκυττάρωσης σε ηπατοκύτταρα που φέρουν τον υποδοχέα της ασιαλογλυκοπρωτεΐνης. [Internet] [Thesis]. Πανεπιστήμιο Πατρών; University of Patras; 2000. [cited 2021 Feb 26]. Available from: http://hdl.handle.net/10442/hedi/12558.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Καλλιντέρη . Λιποσώματα που ενσωματώνουν το ασιαλογλυκοπεπτίδιο A-F2 της φετουΐνης: μελέτη φυσικοχημικής σταθερότητας και ενδοκυττάρωσης σε ηπατοκύτταρα που φέρουν τον υποδοχέα της ασιαλογλυκοπρωτεΐνης. [Thesis]. Πανεπιστήμιο Πατρών; University of Patras; 2000. Available from: http://hdl.handle.net/10442/hedi/12558

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

.