You searched for subject:(arginase).
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1.
Silva, Maria Fernanda Laranjeira da.
Relação entre a localização celular da enzima arginase de Leishmania (Leishmania) amazonensis e seu papel na infecção de macrófagos murinos.
Degree: PhD, Fisiologia Geral, 2010, University of São Paulo
URL: http://www.teses.usp.br/teses/disponiveis/41/41135/tde-21072010-135930/ 
;
► Nos hospedeiros mamíferos, os parasitas do gênero Leishmania vivem nos macrófagos se evadindo de mecanismos microbicidas dessas células, tais como a produção de óxido nítrico…
(more)
▼ Nos hospedeiros mamíferos, os parasitas do gênero Leishmania vivem nos macrófagos se evadindo de mecanismos microbicidas dessas células, tais como a produção de óxido nítrico (NO). A produção de NO pela enzima óxido nítrico sintase induzida (iNOS) nos macrófagos requer L-arginina como substrato, o mesmo aminoácido utilizado pela arginase para produzir ornitina e uréia. Logo, a arginase pode atuar na sobrevivência de Leishmania no hospedeiro competindo com a iNOS, reduzindo a produção de NO, além de seu papel na via de poliaminas, essencial para a replicação dessas células. Com isso, o objetivo desse estudo é elucidar o papel da arginase de L. (L.) amazonensis durante o ciclo de vida do parasita, particularmente, sua função no estabelecimento e na manutenção da infecção da célula hospedeira, e como esse papel seria exercido. Nesse sentido, obtivemos soros policlonais anti-arginase, a partir da arginase recombinante de L. (L.) amazonensis purificada, e esses soros foram utilizados na imunomarcação da enzima em preparações com formas promastigotas e macrófagos infectados com amastigotas de L. (L.) amazonensis. Assim, determinamos a compartimentalização da arginase nos glicossomos tanto na forma promastigota do parasita como na forma amastigota, durante a infecção. Além disso, obtivemos diversos mutantes com a expressão de arginase modificada quanto à quantidade e localização que nos permitiram avaliar a importância da compartimentalização dessa enzima nos glicossomos. Entre esses mutantes temos: superexpressores de arginase, com e sem sinal de endereçamento para glicossomo; parasitas com um alelo de arginase nocauteado e o outro substituído pelo cassete contendo o segmento ddFKBP-ARG, que teriam a expressão de arginase regulada pelo domínio ddFKB sendo nocautes funcionais de arginase; e finalmente, também obtivemos parasitas nocaute nulo de arginase. A análise desses mutantes permitiu conclusões importantes para o conhecimento da fisiologia do parasita e sua relação com o macrófago, revelando que o papel da arginase de Leishmania parece ser muito mais complexo do que o inicialmente postulado, participando na regulação de outras vias metabólicas do próprio parasita e da célula hospedeira. Paralelamente, também determinamos que o sistema ddFKBP é funcional em L. (L.) amazonensis, e assim pode ser utilizado no estudo funcional de outras proteínas importantes para esses parasitas.
In the mammal host, Leishmania parasites live inside macrophages escaping from their microbicidal mechanisms, such as the nitric oxide (NO) production. The macrophage NO production by inducible nitric oxide synthase (iNOS) requires L-arginine as substrate, the same amino acid required by arginase to generate ornithine and urea. So, arginase may play a dual role in Leishmania survival reducing the NO by competing with iNOS, and participating in the polyamines pathway, which is essential for the cells replication. Considering this, the aim of this study is to elucidate the role of L. (L.) amazonensis arginase during the parasite life cycle,…
Advisors/Committee Members: Winter, Lucile Maria Floeter.
Subjects/Keywords: Arginase; Arginase; Glicossomo; Glycosome; Infecção; Infection
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APA (6th Edition):
Silva, M. F. L. d. (2010). Relação entre a localização celular da enzima arginase de Leishmania (Leishmania) amazonensis e seu papel na infecção de macrófagos murinos. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/41/41135/tde-21072010-135930/ ;
Chicago Manual of Style (16th Edition):
Silva, Maria Fernanda Laranjeira da. “Relação entre a localização celular da enzima arginase de Leishmania (Leishmania) amazonensis e seu papel na infecção de macrófagos murinos.” 2010. Doctoral Dissertation, University of São Paulo. Accessed January 19, 2021.
http://www.teses.usp.br/teses/disponiveis/41/41135/tde-21072010-135930/ ;.
MLA Handbook (7th Edition):
Silva, Maria Fernanda Laranjeira da. “Relação entre a localização celular da enzima arginase de Leishmania (Leishmania) amazonensis e seu papel na infecção de macrófagos murinos.” 2010. Web. 19 Jan 2021.
Vancouver:
Silva MFLd. Relação entre a localização celular da enzima arginase de Leishmania (Leishmania) amazonensis e seu papel na infecção de macrófagos murinos. [Internet] [Doctoral dissertation]. University of São Paulo; 2010. [cited 2021 Jan 19].
Available from: http://www.teses.usp.br/teses/disponiveis/41/41135/tde-21072010-135930/ ;.
Council of Science Editors:
Silva MFLd. Relação entre a localização celular da enzima arginase de Leishmania (Leishmania) amazonensis e seu papel na infecção de macrófagos murinos. [Doctoral Dissertation]. University of São Paulo; 2010. Available from: http://www.teses.usp.br/teses/disponiveis/41/41135/tde-21072010-135930/ ;
University of Manitoba
2.
Muleme, Helen.
The role of parasite-derived arginase in murine Leishmania major.
Degree: Immunology, 2010, University of Manitoba
URL: http://hdl.handle.net/1993/3920
► The outcome of infection with Leishmania major depends in part on the balance between arginase and inducible nitric oxide synthase in macrophages. These enzymes compete…
(more)
▼ The outcome of infection with Leishmania major depends in part on the balance between
arginase and inducible nitric oxide synthase in macrophages. These enzymes compete for the substrate L-arginine. Leishmania major also encodes an
arginase gene but, the role of this parasite-derived enzyme in infection remains unclear. We hypothesize that parasite-derived
arginase influences parasite survival and host immune response to L. major. To examine this hypothesis, we employed an
arginase deficient null mutant L. major in in vitro and in vivo experiments. Our results show that deficiency of parasite-derived
arginase impaired parasite proliferation and disease pathogenesis. Increased
arginase activity however neither affected nitric oxide production, nor did it correlate with IL-4 production. Primary infection of normally resistant hosts causes a chronic infection and does not protect them against re-infection. Thus, parasite-derived
arginase is of nutritional importance to L. major, but is not a feasible therapeutic drug target.
Advisors/Committee Members: Uzonna, Jude (Immunology) (supervisor), Soussi Gounni, Abdel (Immunology).
Subjects/Keywords: arginase; leishmaniasis; immunoparasitology
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APA (6th Edition):
Muleme, H. (2010). The role of parasite-derived arginase in murine Leishmania major. (Masters Thesis). University of Manitoba. Retrieved from http://hdl.handle.net/1993/3920
Chicago Manual of Style (16th Edition):
Muleme, Helen. “The role of parasite-derived arginase in murine Leishmania major.” 2010. Masters Thesis, University of Manitoba. Accessed January 19, 2021.
http://hdl.handle.net/1993/3920.
MLA Handbook (7th Edition):
Muleme, Helen. “The role of parasite-derived arginase in murine Leishmania major.” 2010. Web. 19 Jan 2021.
Vancouver:
Muleme H. The role of parasite-derived arginase in murine Leishmania major. [Internet] [Masters thesis]. University of Manitoba; 2010. [cited 2021 Jan 19].
Available from: http://hdl.handle.net/1993/3920.
Council of Science Editors:
Muleme H. The role of parasite-derived arginase in murine Leishmania major. [Masters Thesis]. University of Manitoba; 2010. Available from: http://hdl.handle.net/1993/3920
Queens University
3.
Richmond, Christopher.
Roles of Arginase-1 in Neural Cells
.
Degree: Biomedical and Molecular Sciences, Queens University
URL: http://hdl.handle.net/1974/25912
► Arginase-1 (ARG1) is an essential urea cycle (UC) enzyme that catalyzes the hydrolysis of arginine, proposed to also be involved in pre- and post-natal development…
(more)
▼ Arginase-1 (ARG1) is an essential urea cycle (UC) enzyme that catalyzes the hydrolysis of arginine, proposed to also be involved in pre- and post-natal development and post-injury regeneration of neural cells. Genetic mutations in fundamental regions of ARG1 may cause ARG1 deficiency (ARG1D), a UC disorder (UCD) that results in partial or complete loss of functional ARG1 expression. ARG1D presents with late onset neurological phenotypes in comparison to other UCDs, that include: intellectual and growth retardation, and initial spastic diplegia with progression into paraplegia. Because of prominent arginase-1 expression in the nervous system (NS) during embryonic and post-natal development, subsequent upregulation post-injury to the NS, and unique neurological phenotypes of ARG1D in comparison to other UCDs, we hypothesized that ARG1 has pivotal neurobiological roles.
To investigate this, we crossed nestin-cre and Arg1 loxP-flanked mouse strains, effectively creating neural (n)Arg1 knockout (KO) mice, which should congenitally lack functional arginase-1 expression in neural cells. Cre-loxP recombination of Arg1 was determined by PCR genotyping and characterization of the mouse model included protein and mRNA expression analysis of various tissues to confirm NS specific KO of arginase-1. Subsequently, nArg1 KO mice were compared to control mice for assessment of body weight, blood amino acid levels, and stride gait cycle utilizing DigiGait Imaging Systems during 8 to 26 weeks of age. To assess the role of Arg1 post-injury in neural cells a sciatic nerve crush injury (SNCI) model was used. Functional tests were carried out to assay differences in axon reinnervation by assessing return of lost motor and sensory function of nArg1 KO mice compared to control mice. Results suggest no temporal differences in axon reinnervation post-SNCI based on the rate of return of sensory function. Gait abnormalities reminiscent of those observed in ARG1-deficient patients were absent. Our results suggest that an absence of Arg1 in neural cells does not contribute to the phenotypes of ARG1 deficiency, nor is Arg1 likely to be crucial for post-injury axonal regeneration in this particular model, although return of motor function displayed modest delays compared to control mice.
Subjects/Keywords: Arginase-1
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APA (6th Edition):
Richmond, C. (n.d.). Roles of Arginase-1 in Neural Cells
. (Thesis). Queens University. Retrieved from http://hdl.handle.net/1974/25912
Note: this citation may be lacking information needed for this citation format:
No year of publication.
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Richmond, Christopher. “Roles of Arginase-1 in Neural Cells
.” Thesis, Queens University. Accessed January 19, 2021.
http://hdl.handle.net/1974/25912.
Note: this citation may be lacking information needed for this citation format:
No year of publication.
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Richmond, Christopher. “Roles of Arginase-1 in Neural Cells
.” Web. 19 Jan 2021.
Note: this citation may be lacking information needed for this citation format:
No year of publication.
Vancouver:
Richmond C. Roles of Arginase-1 in Neural Cells
. [Internet] [Thesis]. Queens University; [cited 2021 Jan 19].
Available from: http://hdl.handle.net/1974/25912.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
No year of publication.
Council of Science Editors:
Richmond C. Roles of Arginase-1 in Neural Cells
. [Thesis]. Queens University; Available from: http://hdl.handle.net/1974/25912
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
No year of publication.
4.
Alexandre Lustosa Pereira.
Terapia periodontal não-cirúrgica: avaliação de eficiência por parâmetros clínicos microbianos e salivares.
Degree: 2009, Universidade de Taubaté
URL: http://www.bdtd.unitau.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=439
► Hipótese do estudo: O presente estudo parte do pressuposto de que a terapia periodontal não cirúrgica é eficaz no tratamento da doença periodontal. Objetivos: este…
(more)
▼ Hipótese do estudo: O presente estudo parte do pressuposto de que a terapia periodontal não cirúrgica é eficaz no tratamento da doença periodontal. Objetivos: este estudo de intervenção avaliou a eficácia dos procedimentos de raspagem e aplainamento radicular utilizando como ferramenta de validação parâmetros clínicos, microbianos e salivares em indivíduos diagnosticados com gengivite e periodontite comparando-os a controles saudáveis. Método: Foram alocados no presente estudo 89 indivíduos assim caracterizados: 31 periodontalmente saudáveis (K), 27 com gengivite (G) e 31 com periodontite crônica (P). Avaliou-se os seguintes parâmetros clínicos: índice de placa, índice de sangramento, profundidade de sondagem e nível de inserção clínica. Avaliou-se ainda a presença dos periodontopatógenos Campylobacter rectus, Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tanerella forsythia, Treponema denticola e Prevotella intermedia por meio da reação em cadeia da polimerase (PCR). Finalmente, a presença salivar de arginase e proteína total por meio de espectrofotometria foi também considerada. Todos estes parâmetros foram mensurados em pré e pós terapia periodontal. Tratamento estatístico dos dados intragrupo foi realizada pelo teste de Wilcoxon, enquanto os dados intergrupos foram avaliados pelos testes Mann-Witney e Kruskal-Wallis, adotando-se significância estatística quando p<0,05. Resultados: Verificou-se melhora dos parâmetros clínicos nos grupos G e P após o tratamento (p<0,05). Houve redução da atividade de arginase e proteína total salivares nos grupos P (p<0,05) e G, equiparando-se ao grupo controle (K). T. forsythia reduziu no grupo G (p<0,05), enquanto P. gingivalis, T. denticola, P. intermedia e T. forsythia apresentaram redução no grupo P (p<0,05). Conclusão: O pressuposto apresentado neste estudo foi confirmado, tendo como validação a utilização dos parâmetros clínicos, microbiológicos e salivares.
Hypothesis of the study: the present study assumes that non-surgical periodontal therapy is efficient on the periodontal disease treatment. Aims: this interventional study evaluated the efficacy of the scaling and root planing procedures by using as a tool of validation, clinical, microbiological and salivary parameters in subjects diagnosed with gingivitis and periodontitis comparing them with healthy controls. Methods: In the present study, 89 subjects were characterized as follows: 31 periodontal healthy (K), 27 with gingivitis (G), and 31 with chronic periodontitis (P). The following parameters were evaluated: plaque index, bleeding index, probing depth, and clinical attachment level. The presence of the periodontalpathogens Campylobacter rectus, Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tanerella forsythia, Treponema denticola, and Prevotella intermedia were also evaluated by polymerase chain reaction (PCR). Finally, the presence of salivary arginase and total protein by spectrophotometry were also considered. All these parameters were measured before and after…
Advisors/Committee Members: Marinella Holzhausen, Davi Romeiro Aquino, Luis Carlos Spolidorio, José Roberto Cortelli.
Subjects/Keywords: bacteria; gingivitis; arginase; PERIODONTIA; periodontite; gengivite; bactérias; arginase; periodontitis
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APA (6th Edition):
Pereira, A. L. (2009). Terapia periodontal não-cirúrgica: avaliação de eficiência por parâmetros clínicos microbianos e salivares. (Thesis). Universidade de Taubaté. Retrieved from http://www.bdtd.unitau.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=439
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Pereira, Alexandre Lustosa. “Terapia periodontal não-cirúrgica: avaliação de eficiência por parâmetros clínicos microbianos e salivares.” 2009. Thesis, Universidade de Taubaté. Accessed January 19, 2021.
http://www.bdtd.unitau.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=439.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Pereira, Alexandre Lustosa. “Terapia periodontal não-cirúrgica: avaliação de eficiência por parâmetros clínicos microbianos e salivares.” 2009. Web. 19 Jan 2021.
Vancouver:
Pereira AL. Terapia periodontal não-cirúrgica: avaliação de eficiência por parâmetros clínicos microbianos e salivares. [Internet] [Thesis]. Universidade de Taubaté; 2009. [cited 2021 Jan 19].
Available from: http://www.bdtd.unitau.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=439.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Pereira AL. Terapia periodontal não-cirúrgica: avaliação de eficiência por parâmetros clínicos microbianos e salivares. [Thesis]. Universidade de Taubaté; 2009. Available from: http://www.bdtd.unitau.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=439
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Punjabi University
5.
Kaur, Gurnoor.
Purification and characterization of arginase from
neurospora sp for the development of arginine biosensor.
Degree: 2011, Punjabi University
URL: http://shodhganga.inflibnet.ac.in/handle/10603/2897
► L-arginine is a naturally occurring basic amino acid that participates in many important biochemical reactions associated with normal physiology of the organism. It has been…
(more)
▼ L-arginine is a naturally occurring basic amino
acid that participates in many important biochemical reactions
associated with normal physiology of the organism. It has been
considered the most potent Nutraceutical ever discovered, due to
its powerful healing properties, and is referred to by scientists
as the Miracle Molecule. Since this compound is essential for
protein synthesis and has a variety of other important advantages
apart from being necessary for a host of other biochemical
reactions, it is important to detect L-arginine in physiological
fluids. In foods, Arginine is used as a flavor-providing agent. Its
detection in foods is imperative as a control measure for quality
check in foods such as beverages, juices & wine. The present
research work was carried out for the development of potentiometric
and fiber-optic biosensors for detecting Arginine in clinical and
food samples. The enzyme Arginase was extracted from fungus
Neurospora crassa and recombinant enzyme was obtained by expression
in E. coli. The recombinant enzyme production was carried out at
shake-flask level followed by optimizing media for its production
and later production was scaled up to fermentor scale. Following
this, the enzyme was purified and later immobilized by various
immobilization techniques and employed as biocomponent for
development of Biosensors (Potentiometric and Fiber-optic) for
monitoring Arginine. Sol-gel modified potentiometric biosensor was
developed by means of semi-quantitative analysis on nylon membranes
and later by coupling them to NH4 + ISE as a potentiometric
transducer for quantitative analysis. Validation studies were
carried out by spiking the samples and differences obtained were
found to be statisticslly insignificant. Both clinical and food
samples were monitored for arginine content. The developed
biosensor is sensitive, rapid, easy-to-use and portable and the
detection range (nano-level detection) achieved is way better than
existing arginine biosensors. The storage stability of the
biocomponent is superior and enhanced than conventional arginine
biosensor techniques.
Summary, Publications, References,
Appendix
Advisors/Committee Members: Verma, Neelam, Wheatley, Denys N.
Subjects/Keywords: Arginine; Recombinant; Arginase; Biosensor; Potentiometric; Fiber-optics
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APA ·
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APA (6th Edition):
Kaur, G. (2011). Purification and characterization of arginase from
neurospora sp for the development of arginine biosensor. (Thesis). Punjabi University. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/2897
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Kaur, Gurnoor. “Purification and characterization of arginase from
neurospora sp for the development of arginine biosensor.” 2011. Thesis, Punjabi University. Accessed January 19, 2021.
http://shodhganga.inflibnet.ac.in/handle/10603/2897.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Kaur, Gurnoor. “Purification and characterization of arginase from
neurospora sp for the development of arginine biosensor.” 2011. Web. 19 Jan 2021.
Vancouver:
Kaur G. Purification and characterization of arginase from
neurospora sp for the development of arginine biosensor. [Internet] [Thesis]. Punjabi University; 2011. [cited 2021 Jan 19].
Available from: http://shodhganga.inflibnet.ac.in/handle/10603/2897.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Kaur G. Purification and characterization of arginase from
neurospora sp for the development of arginine biosensor. [Thesis]. Punjabi University; 2011. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/2897
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Iceland
6.
Pétur Rafnsson 1988-.
Glucose as a factor of importance for regulating arginase activity in red blood cells
.
Degree: 2014, University of Iceland
URL: http://hdl.handle.net/1946/18038
► Background: Nitric oxide (NO) is an important biological messenger in the cardiovascular system. NO causes vasodilation, inhibits inflammation and platelet activation. The bioavailability of NO…
(more)
▼ Background: Nitric oxide (NO) is an important biological messenger in the cardiovascular system. NO causes vasodilation, inhibits inflammation and platelet activation. The bioavailability of NO is reduced in various cardiovascular diseases including atherosclerosis and diabetes compared to a healthy state. NO is formed by the enzyme nitric oxide synthase (NOS) which can be found throughout the vasculature. The amino acid L-arginine is a substrate in the reaction of NO production. The mechanism of reduced bioavailability of NO is not completely understood but it is thought to be mainly reduced production of NO and increased inactivation of NO. Emerging evidence suggest that the enzyme arginase is a major contributor to this mechanism. Arginase also uses L-arginine as a substrate to produce urea and ornithine. Experimental data demonstrate that arginase activity is increased in rats with type 2 diabetes compared with healthy controls. Glucose has been reported to regulate arignase activity in endothelial cells. Red blood cells (RBCs) contain high levels of arginase which regulates NO but this regulation in RBCs and it‘s function in these cells is unknown. The aim of this study was to test the hypothesis if there is a difference in arginase activity in RBCs between healthy individuals and patients with type 2 diabetes. Another objective was to investigate if high glucose concentration would affect arginase activity in red blood cells.
Methods: Red blood cells were extracted from healthy individuals and patients with type 2 diabetes . These cells were incubated for 24 and 48 hours in a buffer solution containing 0 mM, 5.5 mM or 25 mM glucose. Arginase activity was determined by measuring the amount of urea produced using spectrophotometry.
Results: Arginase activity at baseline, (i.e. 0 hours) of incubation did not differ between healthy individuals and patients with type 2 diabetes. Arginase activity decreased with time in both groups. Arginase activity reached a steady-state at 24 hours of incubation. There was no significant difference between arginase activity whether they were incubated with 5.5 mM glucose concentration or 25mM glucose concentration.
Discussion: According to the results of this study glucose does not effect arginase activity in red blood cells. There is not a significant difference in arginase activity in red blood cells between healthy subjects and patients with type 2 diabetes.
Keywords: Nitric oxide (NO), nitric oxide synthase (NOS), arginase, arginase activity, diabetes type 2, glucose, incubation.
Subjects/Keywords: Læknisfræði;
Sykursýki;
Blóðkorn;
Nitric oxide;
Arginase activity
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APA (6th Edition):
1988-, P. R. (2014). Glucose as a factor of importance for regulating arginase activity in red blood cells
. (Thesis). University of Iceland. Retrieved from http://hdl.handle.net/1946/18038
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
1988-, Pétur Rafnsson. “Glucose as a factor of importance for regulating arginase activity in red blood cells
.” 2014. Thesis, University of Iceland. Accessed January 19, 2021.
http://hdl.handle.net/1946/18038.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
1988-, Pétur Rafnsson. “Glucose as a factor of importance for regulating arginase activity in red blood cells
.” 2014. Web. 19 Jan 2021.
Vancouver:
1988- PR. Glucose as a factor of importance for regulating arginase activity in red blood cells
. [Internet] [Thesis]. University of Iceland; 2014. [cited 2021 Jan 19].
Available from: http://hdl.handle.net/1946/18038.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
1988- PR. Glucose as a factor of importance for regulating arginase activity in red blood cells
. [Thesis]. University of Iceland; 2014. Available from: http://hdl.handle.net/1946/18038
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
7.
Daher Antonio Queiroz.
Avaliação da atividade da arginase na saliva de indivíduos submetidos a implantes dentários.
Degree: 2008, Universidade de Taubaté
URL: http://www.bdtd.unitau.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=464
► A saliva humana possui diversas enzimas que apresentam atividades específicas e algumas delas podem estar envolvidas no processo inflamatório que acometem os implantes dentários. A…
(more)
▼ A saliva humana possui diversas enzimas que apresentam atividades específicas e algumas delas podem estar envolvidas no processo inflamatório que acometem os implantes dentários. A arginase é uma enzima hidrolítica que utiliza a L-arginina como substrato para formação da L-ornitina e uréia. Acredita-se que um aumento na produção de arginase possa levar à uma redução na produção de óxido nítrico, consequentemente aumentando a suscetibilidade à infecção bacteriana. Considerando a hipótese de que o número de implantes nos indivíduos e o fumo possam alterar a produção de arginase e que o mesmo é considerado um fator de risco para o sucesso da terapia com implantes, o presente estudo objetivou avaliar o efeito de ambas as variáveis sobre a atividade da arginase salivar (AS) em pacientes portadores de implantes dentários. Amostras de saliva de 102 indivíduos foram coletadas: 26 não-fumantes e sem implantes dentais (A), 28 pacientes com até 4 implantes dentais e não-fumantes (B), 29 pacientes com cinco ou mais implantes dentais e não-fumantes (C), dez fumantes com implantes (D) e nove fumantes sem implantes (E). Os níveis de AS foram expressos em U/mg proteína e determinados por espectrofotometria através da mensuração da L-ornitina e da proteína salivar. Houve um aumento significativo dos níveis de AS no grupo D (64.26) em relação aos grupos A (10.72, p=0), B (10.66, p=0), C (11.21, p=0) e E (13.66, p=0). Não houve diferença estatisticamente significante entre os grupos A, B, C e E (p>0,05). Dentro dos limites do presente estudo, pode-se concluir que a atividade da arginase salivar encontra-se elevada em indivíduos fumantes portadores de implante dentário, sugerindo um possível mecanismo pelo qual o fumo pode levar ao insucesso desta terapia reabilitadora.
Human saliva possesses many enzymes with activity and some of them might be involved in the inflammatory process of dental implants. Arginase is a hydrolytic enzyme that catalyses L- arginine into ornithine and urea. Its known that a high production of arginase can decreased the production of nitric oxide, consequently leading to bacterial infection susceptibility. To regard the hypothesis of number of implants and smoking might change the production of arginase and considering that smoking is a risk factor of implant therapy, the aim of this study was to evaluate the effect of both on the activity of arginase in saliva of subjects with dental implants. Saliva of 102 subjects were collected: 26 non-smoking and without dental implants (A), 28 with 4 dental implants or less and non-smoking (B), 29 with five dental implants or more and non-smoking (C), ten smokers with implants (D) and nine smokers and without dental implants (E). The arginase levels were expressed in U/mg and analyzed by spectophotometry and it was determined by measuring the Lornitine formation from L-arginine. The result showed that the mean values of arginase in group D (64.26) were statistically different among groups A (10.72, p=0), B (10.66, p=0), C (11.21, p=0) e D (13.66, p=0). There was no…
Advisors/Committee Members: Marinella Holzhausen, Wilson Abrao Saad, Anuar Antônio Xible, José Roberto Cortelli.
Subjects/Keywords: saliva; implantes dentários; arginase; arginase; saliva; smoke; óxido nítrico; fumo; nitric oxide; dental implants; ODONTOLOGIA
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APA (6th Edition):
Queiroz, D. A. (2008). Avaliação da atividade da arginase na saliva de indivíduos submetidos a implantes dentários. (Thesis). Universidade de Taubaté. Retrieved from http://www.bdtd.unitau.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=464
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Queiroz, Daher Antonio. “Avaliação da atividade da arginase na saliva de indivíduos submetidos a implantes dentários.” 2008. Thesis, Universidade de Taubaté. Accessed January 19, 2021.
http://www.bdtd.unitau.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=464.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Queiroz, Daher Antonio. “Avaliação da atividade da arginase na saliva de indivíduos submetidos a implantes dentários.” 2008. Web. 19 Jan 2021.
Vancouver:
Queiroz DA. Avaliação da atividade da arginase na saliva de indivíduos submetidos a implantes dentários. [Internet] [Thesis]. Universidade de Taubaté; 2008. [cited 2021 Jan 19].
Available from: http://www.bdtd.unitau.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=464.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Queiroz DA. Avaliação da atividade da arginase na saliva de indivíduos submetidos a implantes dentários. [Thesis]. Universidade de Taubaté; 2008. Available from: http://www.bdtd.unitau.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=464
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Universidade Estadual de Campinas
8.
Cecilio, Carla Aparecida, 1967-.
Imunidade e envelhecimento : avaliação da produção de arginase e sintase do óxido nítrico por macrófagos de camundongos.
Degree: 2011, Universidade Estadual de Campinas
URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/317405
► Abstract: Given that the age-dependent changes in the innate immune system are poorly studied in the present study we investigated the activity of iNOS and…
(more)
▼ Abstract: Given that the age-dependent changes in the innate immune system are poorly studied in the present study we investigated the activity of iNOS and
arginase, as well as cytokine production in macrophages from BALB/c young (8 weeks old ) and old (72 weeks old). Initial assessments carried out on peritoneal macrophages (PM?) or elicited from naive BALB/c mice showed that these young phagocytes have detectable levels of
arginase activity, even in the absence of inducing stimuli. Only IL-4 induced increased
arginase activity in these cells, while LPS and IFN-? induced iNOS activity. LPS inhibited the
arginase activity induced by IL-4 in PM? and macrophages derived from bone marrow (BMM?). IL-4 inhibited NO production in naïve PM? and BMM? in MO, but had no effect on M?P elicited. Phagocytosis of zymosan or apoptotic lymphocytes, but not from Leishmania major, resulting in increased
arginase activity in elicited macrophages and only the phagocytosis of zymosan induced an increase in
arginase activity in naïve macrophages. Phagocytosis of any of the tested particles resulted in an increase in
arginase activity when IL4 was added to sub-optimal dose to cultures of PM?, the addition of sub-optimal dose of IFN-? resulted in increased
arginase activity only in PM? of BALB/c elicited that phagocytized Leishmania major. Comparatively, PM? of C57BL/6 showed
arginase activity much lower than the BALB/c after the phagocytosis of any of the tested particles. Phagocytosis of zymosan or apoptotic lymphocytes, but not L. major, induced
arginase activity in PM? elicited from C57BL/6 and the addition of IFN-? significantly increased
arginase activity in these cells. Just as in BALB/c, PM? C57BL/6 iNOS showed no activity after phagocytosis of any particles tested either in the presence or absence of inflammatory stimuli. Our results also show that aging alters some of the activities of macrophages from BALB/c. Similarly, we found that PM? of young mice showed activities iNOS and
arginase higher than PM? of old mice. BMM? of young and old mice did not differ significantly in iNOS activity, but
arginase activity was higher in BMM? in older animals. The expression of
arginase-induced IL-4 was higher in PM? of naïve young mice, but was also high in M?P elicited AND BMM? of young and old mice. No difference was observed in the expression of iNOS induced by IFN-? in both PM? and BMM? of BALB/c young and old. The production of IL-1 ? induced by LPS was higher in elicited macrophages from aged mice and TNF-? was higher in elicited macrophages from young mice. Stimulation of BMM? with LPS only resulted in production of TNF-?, whose levels were higher in cells of aged mice. Our results suggest age-dependent differences in tissue macrophages and differentiated in vitro. With respect to tissue macrophages, the most notable changes were the age-dependent reduction of and activity of iNOS. Given the importance of these two mediators in the efficient elimination of pathogens, the reduction in macrophages of aged mice may explain the increased…
Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS (CRUESP), Tamashiro, Wirla Maria da Silva Cunha, 1950- (advisor), Universidade Estadual de Campinas. Instituto de Biologia (institution), Programa de Pós-Graduação em Genética e Biologia Molecular (nameofprogram), Giorgio, Selma (committee member), Verinaud, Liana Maria Cardoso (committee member), Ferreira, Heloisa Helena de de Araújo (committee member), Barreira, Maria Cristina Roque (committee member).
Subjects/Keywords: Envelhecimento; Camundongo; Macrofagos; Arginase; Óxido nítrico; Aging; Mice; Macrophages; Arginase; Nitric oxide
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APA (6th Edition):
Cecilio, Carla Aparecida, 1. (2011). Imunidade e envelhecimento : avaliação da produção de arginase e sintase do óxido nítrico por macrófagos de camundongos. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/317405
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Cecilio, Carla Aparecida, 1967-. “Imunidade e envelhecimento : avaliação da produção de arginase e sintase do óxido nítrico por macrófagos de camundongos.” 2011. Thesis, Universidade Estadual de Campinas. Accessed January 19, 2021.
http://repositorio.unicamp.br/jspui/handle/REPOSIP/317405.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Cecilio, Carla Aparecida, 1967-. “Imunidade e envelhecimento : avaliação da produção de arginase e sintase do óxido nítrico por macrófagos de camundongos.” 2011. Web. 19 Jan 2021.
Vancouver:
Cecilio, Carla Aparecida 1. Imunidade e envelhecimento : avaliação da produção de arginase e sintase do óxido nítrico por macrófagos de camundongos. [Internet] [Thesis]. Universidade Estadual de Campinas; 2011. [cited 2021 Jan 19].
Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/317405.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Cecilio, Carla Aparecida 1. Imunidade e envelhecimento : avaliação da produção de arginase e sintase do óxido nítrico por macrófagos de camundongos. [Thesis]. Universidade Estadual de Campinas; 2011. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/317405
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
9.
Aristóteles, Luciana Ritha de Cassia Rolim Barbosa.
Modulação da mecânica do tecido pulmonar periférico e da resposta do estresse oxidativo pela inibição da arginase e da iNOS em modelo experimental de inflamação crônica pulmonar.
Degree: Mestrado, Processos Inflamatórios e Alérgicos, 2012, University of São Paulo
URL: http://www.teses.usp.br/teses/disponiveis/5/5165/tde-31072012-113923/
;
► Introdução: A importância do parênquima pulmonar na fisiopatologia da asma tem sido recentemente enfatizada, particularmente nos pacientes com asma grave e de difícil controle. O…
(more)
▼ Introdução: A importância do parênquima pulmonar na fisiopatologia da asma tem sido recentemente enfatizada, particularmente nos pacientes com asma grave e de difícil controle. O óxido nítrico (NO) é um importante modulador da resposta contrátil, inflamatória e de remodelamento pulmonar que ocorrem na asma. Embora seu papel na modulação em vias aéreas proximais e distais já tenha sido estabelecido, seus efeitos no parênquima pulmonar foram pouco investigados. Objetivos: Avaliar se a inibição da
arginase 2, por intermédio do tratamento com N-hydroxy-nor-L-arginine (nor-NOHA), e da iNOS, por intermédio da administração de 1400W, pode modular a resposta constritora de parênquima distal, o estresse oxidativo, a expressão de iNOS, assim como a atividade da
arginase, em modelo de inflamação alérgica crônica pulmonar em cobaias. Métodos: Os animais foram expostos a sete inalações com soro fisiológico ou com ovoalbumina em doses crescentes (1~5mg/ml- 4 semanas) e tratados com 1400W (2mg/Kg ip. diariamente) iniciando após a 7ª inalação e nor- NOHA (10M - infusão no banho durante a avaliação da mecânica oscilatória), ou associação de 1400W + nor-NOHA (administrado conforme descrito anteriormente). Setenta e duas horas após a sétima inalação os animais foram anestesiados, exsanguinados e a foi realizada a avaliação da mecânica oscilatória do parênquima pulmonar, sendo obtidos os valores de resistência e elastância tecidual na condição basal e após desafio (0,1% de ovoalbumina). Em seguida, os fragmentos de tecido pulmonar periférico foram fixados em solução de formolaldeído a 4%, por 48 horas. Depois de terminada a fixação, o material foi submetido às técnicas histológicas habituais com parafina, para obtenção de cortes de 4m de espessura. Os cortes foram corados para Hematoxilina e Eosina e utilizando técnica de imunohistoquímica foram avaliados o número de células iNOS positivas, a expressão de PGF2, do fator de transcrição NF-kB e de
arginase 2 no septo alveolar, por intermédio de avaliação morfométrica. A expressão de
arginase 2, NF-kB e PGF2 nos fragmentos de tecido pulmonar periférico foram avaliados em aumento 400X utilizando o programa Image-Pro Plus 4.5v Image Analysis System e os resultados foram expressos em porcentagem. Em relação ao número de células iNOS positivas nos fragmentos pulmonares, foi utilizada a técnica de contagem de pontos, determinada pelo número de pontos que coincidiam em células positivas em cada campo dividido pelo número de pontos que incidiam no tecido pulmonar, no aumento de 1000x. Foram analisados 10 campos por corte, selecionados de forma randômica. Os resultados foram expressos como células por unidade de área (104m2). Para a avaliação da atividade da
arginase 2, foi utilizado o método descrito no kit da BioAssay Systems que utiliza um cromogênio, que forma um complexo corado com uréia, produzido na reação de
arginase. A determinação da atividade da
arginase 2 envolveu a medida da velocidade de reação, expressa em termos de atividade por miligrama de proteína (U/mg). Resultados: Não houve…
Advisors/Committee Members: Tibério, Iolanda de Fátima Lopes Calvo.
Subjects/Keywords: Arginase; Arginase; Chronic allergic inflammation; Estresse oxidativo; Inflamação alérgica crônica; iNOS; iNOS; NF-kB; NF-kB; Oxidative stress
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APA (6th Edition):
Aristóteles, L. R. d. C. R. B. (2012). Modulação da mecânica do tecido pulmonar periférico e da resposta do estresse oxidativo pela inibição da arginase e da iNOS em modelo experimental de inflamação crônica pulmonar. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/5/5165/tde-31072012-113923/ ;
Chicago Manual of Style (16th Edition):
Aristóteles, Luciana Ritha de Cassia Rolim Barbosa. “Modulação da mecânica do tecido pulmonar periférico e da resposta do estresse oxidativo pela inibição da arginase e da iNOS em modelo experimental de inflamação crônica pulmonar.” 2012. Masters Thesis, University of São Paulo. Accessed January 19, 2021.
http://www.teses.usp.br/teses/disponiveis/5/5165/tde-31072012-113923/ ;.
MLA Handbook (7th Edition):
Aristóteles, Luciana Ritha de Cassia Rolim Barbosa. “Modulação da mecânica do tecido pulmonar periférico e da resposta do estresse oxidativo pela inibição da arginase e da iNOS em modelo experimental de inflamação crônica pulmonar.” 2012. Web. 19 Jan 2021.
Vancouver:
Aristóteles LRdCRB. Modulação da mecânica do tecido pulmonar periférico e da resposta do estresse oxidativo pela inibição da arginase e da iNOS em modelo experimental de inflamação crônica pulmonar. [Internet] [Masters thesis]. University of São Paulo; 2012. [cited 2021 Jan 19].
Available from: http://www.teses.usp.br/teses/disponiveis/5/5165/tde-31072012-113923/ ;.
Council of Science Editors:
Aristóteles LRdCRB. Modulação da mecânica do tecido pulmonar periférico e da resposta do estresse oxidativo pela inibição da arginase e da iNOS em modelo experimental de inflamação crônica pulmonar. [Masters Thesis]. University of São Paulo; 2012. Available from: http://www.teses.usp.br/teses/disponiveis/5/5165/tde-31072012-113923/ ;
10.
Nzoumbou-Boko, Romaric.
Caractérisation d’une voie Immunomodulatrice impliquant l’arginase dans les Trypanosomoses : Characterization of an immunomodulatory pathway involving arginase in Trypanosomiasis.
Degree: Docteur es, Sciences, technologie, santé. Microbiologie-Immunologie, 2013, Université de Bordeaux Segalen
URL: http://www.theses.fr/2013BOR22053
► Une nouvelle voie d’immunomodulation, l’induction de l’arginase par les trypanosomes chez leurs hôtes, a été identifiée et caractérisée. Pour éviter la réponse cytotoxique de l’activation…
(more)
▼ Une nouvelle voie d’immunomodulation, l’induction de l’arginase par les trypanosomes chez leurs hôtes, a été identifiée et caractérisée. Pour éviter la réponse cytotoxique de l’activation « classique » M1 des macrophages et bénéficier de leur activation « alternative » M2, les parasites induisent l’arginase, qui produit la L-ornithine, indispensable à leur développement. Cette voie d’immunomodulation mise en évidence chez la souris infestée par son parasite naturel, Trypanosoma musculi, est également présente dans d’autres trypanosomoses, en particulier la trypanosomose humaine africaine (THA). Une augmentation de l’arginase, retrouvée dans le sérum de patients trypanosomés, se normalise après un traitement efficace. T. brucei gambiense, parasite de l’homme, induit l’arginase au niveau des macrophages murins et des leucocytes humains. T. lewisi, parasite du rat, induit également l’arginase. Au cours de leur longue coévolution avec leurs hôtes, les trypanosomes extracellulaires ont sélectionné un procédé favorisant leur croissance, l’induction de l’arginase, par des facteurs d’excrétion/sécrétion. Nous avons produit un anticorps monoclonal dirigé contre ce facteur inducteur. Il bloque l’induction de l’arginase par T. musculi in vitro et in vivo. Chez la souris infectée, son injection diminue considérablement la parasitémie. Il a permis l’identification du facteur inducteur, une kinésine orpheline. Cet anticorps, inhibant l’induction de l’arginase par différents trypanosomes, reconnaîtrait une région conservée de la kinésine induisant l’arginase. Cette kinésine se lie à des récepteurs de la membrane des macrophages. In vitro, l’addition de mannose à des co-cultures macrophages-parasites bloque l’induction de l’arginase et la multiplication des parasites. Chez la souris infestée par T. musculi, l’injection de mannose diminue la parasitémie, qui est également réduite chez les souris Mrc1-/-, KO pour le récepteur mannose. L’utilisation de molécules ciblant la voie inductrice de l’arginase et/ou ce récepteur peut représenter une nouvelle approche thérapeutique dans les trypanosomoses.
Arginase induction, a mechanism of immunomodulation elaborated by trypanosomes has been identified. To avoid cytotoxic classical M1 macrophage activation, trypanosomes induce alternative M2 macrophage activation, which leads to L-ornithine production, essential for parasite growth. This immunomodulation pathway has been evidenced in a natural murine trypanosomiasis provoked by Trypanosoma musculi. This mechanism is also evidenced in human African trypanosomiasis (HAT). An increase in serum arginase is measured in HAT patients. A return to normal values is obtained after an efficacious treatment. Trypanosoma brucei gambiense, the causative agent of HAT, induces arginase in mouse macrophages and human leucocytes. T. lewisi, a rat parasite, also induces macrophage arginase.During host-parasite co-evolution, extracellular trypanosomes have selected a growth promoting mechanism, macrophage arginase induction by excreted secreted factor (ESF).…
Advisors/Committee Members: Vincendeau, Philippe (thesis director), Silla, Sembella (thesis director).
Subjects/Keywords: Trypanosomes; Trypanosomoses; Macrophage; Macrophage activation; Arginase; Kinésine; Récepteur mannose; Trypanosomes; Trypanosomoses; Macrophage; Macrophage activation; Arginase; Kinesin; Mannose receptor
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APA ·
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APA (6th Edition):
Nzoumbou-Boko, R. (2013). Caractérisation d’une voie Immunomodulatrice impliquant l’arginase dans les Trypanosomoses : Characterization of an immunomodulatory pathway involving arginase in Trypanosomiasis. (Doctoral Dissertation). Université de Bordeaux Segalen. Retrieved from http://www.theses.fr/2013BOR22053
Chicago Manual of Style (16th Edition):
Nzoumbou-Boko, Romaric. “Caractérisation d’une voie Immunomodulatrice impliquant l’arginase dans les Trypanosomoses : Characterization of an immunomodulatory pathway involving arginase in Trypanosomiasis.” 2013. Doctoral Dissertation, Université de Bordeaux Segalen. Accessed January 19, 2021.
http://www.theses.fr/2013BOR22053.
MLA Handbook (7th Edition):
Nzoumbou-Boko, Romaric. “Caractérisation d’une voie Immunomodulatrice impliquant l’arginase dans les Trypanosomoses : Characterization of an immunomodulatory pathway involving arginase in Trypanosomiasis.” 2013. Web. 19 Jan 2021.
Vancouver:
Nzoumbou-Boko R. Caractérisation d’une voie Immunomodulatrice impliquant l’arginase dans les Trypanosomoses : Characterization of an immunomodulatory pathway involving arginase in Trypanosomiasis. [Internet] [Doctoral dissertation]. Université de Bordeaux Segalen; 2013. [cited 2021 Jan 19].
Available from: http://www.theses.fr/2013BOR22053.
Council of Science Editors:
Nzoumbou-Boko R. Caractérisation d’une voie Immunomodulatrice impliquant l’arginase dans les Trypanosomoses : Characterization of an immunomodulatory pathway involving arginase in Trypanosomiasis. [Doctoral Dissertation]. Université de Bordeaux Segalen; 2013. Available from: http://www.theses.fr/2013BOR22053
11.
Jisha, Jose.
Biochemical effects of cadmium, salinity and temperature on the catabolism of proteins and purines in oreochromis mossambicus (Peters).
Degree: 2014, Cochin University of Science and Technology
URL: http://dyuthi.cusat.ac.in/purl/4921
► In the present investigation, three important stressors: cadmium ion (Cd++), salinity and temperature were selected to study their effects on protein and purine catabolism of…
(more)
▼ In the present investigation, three important stressors: cadmium ion (Cd++), salinity and temperature were selected to study their effects on protein and purine catabolism of O. mossambicus. Cadmium (Cd) is a biologically nonessential metal that can be toxic to aquatic animals. Cadmium is a trace element which is a common constituent of industrial effluents. It is a non-nutrient metal and toxic to fish even at low concentrations. Cadmium ions accumulate in sensitive organs like gills, liver, and kidney of fish in an unregulated manner . Thus; the toxic effects of cadmium are related to changes in natural physiological and biochemical processes in organism. The mechanics of osmoregulation (i.e. total solute and water regulation) are reasonably well understood (Evans, 1984, 1993), and most researchers agree that salinities that differ from the internal osmotic concentration of the fish must impose energetic regulatory costs for active ion transport. There is limited information on protein and purine catabolism of euryhaline fish during salinity adaptation. Within a range of non-lethal temperatures, fishes are generally able to cope with gradual temperature changes that are common in natural systems. However, rapid increases or decreases in ambient temperature may result in sub lethal physiological and behavioral responses. The catabolic pathways of proteins and purines are important biochemical processes. The results obtained signifies that O. mossambicus when exposed to different levels of cadmium ion, salinity and temperature show great variation in the catabolism of proteins and purines. The organism is trying to attain homeostasis in the presence of stressors by increasing or decreasing the activity of certain enzymes. The present study revealed that the protein and purine catabolism in O. mossambicus is sensitive to environmental stressors.
Cochin University of Science & Technology
Subjects/Keywords: catabolism of proteins; purines; oreochromis mossambicus; Activity of arginase; AMP deaminase
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APA (6th Edition):
Jisha, J. (2014). Biochemical effects of cadmium, salinity and temperature on the catabolism of proteins and purines in oreochromis mossambicus (Peters). (Thesis). Cochin University of Science and Technology. Retrieved from http://dyuthi.cusat.ac.in/purl/4921
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Jisha, Jose. “Biochemical effects of cadmium, salinity and temperature on the catabolism of proteins and purines in oreochromis mossambicus (Peters).” 2014. Thesis, Cochin University of Science and Technology. Accessed January 19, 2021.
http://dyuthi.cusat.ac.in/purl/4921.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Jisha, Jose. “Biochemical effects of cadmium, salinity and temperature on the catabolism of proteins and purines in oreochromis mossambicus (Peters).” 2014. Web. 19 Jan 2021.
Vancouver:
Jisha J. Biochemical effects of cadmium, salinity and temperature on the catabolism of proteins and purines in oreochromis mossambicus (Peters). [Internet] [Thesis]. Cochin University of Science and Technology; 2014. [cited 2021 Jan 19].
Available from: http://dyuthi.cusat.ac.in/purl/4921.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Jisha J. Biochemical effects of cadmium, salinity and temperature on the catabolism of proteins and purines in oreochromis mossambicus (Peters). [Thesis]. Cochin University of Science and Technology; 2014. Available from: http://dyuthi.cusat.ac.in/purl/4921
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Queens University
12.
Price, Phillipe.
CRISPR/Cas9-Mediated Gene Editing in the Arginase-1 Deficient Mouse Genome
.
Degree: Biochemistry, 2015, Queens University
URL: http://hdl.handle.net/1974/13626
► Arginase-1 deficiency is a rare, autosomal recessive disorder of the urea cycle that normally converts ammonia to less toxic urea. Symptoms include neurodegeneration, spastic diplegia…
(more)
▼ Arginase-1 deficiency is a rare, autosomal recessive disorder of the urea cycle that normally converts ammonia to less toxic urea. Symptoms include neurodegeneration, spastic diplegia and mental retardation. We designed a Clustered Regularly Interspaced Palindromic Repeat (CRISPR)/Cas9 system to correct an induced Arg1 exon 7 and 8 deletion (Arg1∆) that produces arginase-1 deficiency in mice.
Three cell lines were generated to study this disease. The first was primary mouse embryonic fibroblasts isolated from Arg1∆-mice (Arg1∆-PMEF). The second was mouse induced-pluripotent stem cells converted from the aforementioned PMEFs (Arg1∆-miPSC). The third was human embryonic 293T kidney cells with introduced Arg1∆-transgenic DNA (Arg1∆-HEK). These three cell lines were transfected with a targeted Cas9:guide-RNA (gRNA) plasmid. PCR amplicons were subjected to a heteroduplex cleavage assay and sequenced to confirm Cas9 cleavage just upstream of the exon 7 and 8 deletion site.
DNA cleavage by Cas9:gRNA can promote homologous recombination with a single-stranded oligodeoxynucleotide (ssODN) to produce small edits in the genome of miPSCs. This mimicked an editing strategy that could be used to correct single nucleotide mutations in Arg1. Homologous recombination was also used to introduce a targeting vector containing both wild-type exon 7 and 8 cDNA and a removable positive-negative selection cassette in miPSCs. PCR analysis and sequencing of “targeted” (positively selected) cells has shown the presence of the repair vector in the correct location in Arg1. Attempts were made to subsequently remove the selection cassette by PiggyBac transposase. The “repaired” (negatively selected) miPSCs were probed by PCR to test for the correct incorporation of exons 7 and 8, showing unclear results. These cells were converted to hepatocyte-like cells showing the correct morphology and staining positive for glycogen. Future tests will be conducted to introduce these hepatocyte-like cells into Arg1-deficient mice. These experiments will serve as a functional model to test gene editing in arginase-1 deficiency. Because the CRISPR/Cas9 system can be designed to target any gene precisely, it has far-reaching applications, particularly for genetic disorders.
Subjects/Keywords: Arginase-1 Deficiency
;
Induced Pluripotent Stem Cells
;
Gene Editing
;
CRISPR/Cas9
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APA (6th Edition):
Price, P. (2015). CRISPR/Cas9-Mediated Gene Editing in the Arginase-1 Deficient Mouse Genome
. (Thesis). Queens University. Retrieved from http://hdl.handle.net/1974/13626
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Price, Phillipe. “CRISPR/Cas9-Mediated Gene Editing in the Arginase-1 Deficient Mouse Genome
.” 2015. Thesis, Queens University. Accessed January 19, 2021.
http://hdl.handle.net/1974/13626.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Price, Phillipe. “CRISPR/Cas9-Mediated Gene Editing in the Arginase-1 Deficient Mouse Genome
.” 2015. Web. 19 Jan 2021.
Vancouver:
Price P. CRISPR/Cas9-Mediated Gene Editing in the Arginase-1 Deficient Mouse Genome
. [Internet] [Thesis]. Queens University; 2015. [cited 2021 Jan 19].
Available from: http://hdl.handle.net/1974/13626.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Price P. CRISPR/Cas9-Mediated Gene Editing in the Arginase-1 Deficient Mouse Genome
. [Thesis]. Queens University; 2015. Available from: http://hdl.handle.net/1974/13626
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Otago
13.
Nair, Shiva Madhwan.
The Mechanisms of Neuroprotection by Melatonin in Cerebral Ischaemia Reperfusion
.
Degree: 2011, University of Otago
URL: http://hdl.handle.net/10523/1956
► Stroke is a major cause of death and the highest cause of morbidity worldwide. In NZ, the elderly and Maori and Pacific Island ethnicities are…
(more)
▼ Stroke is a major cause of death and the highest cause of morbidity worldwide. In NZ, the elderly and Maori and Pacific Island ethnicities are most affected. With failure of many neuroprotectant agents, current clinical therapy is limited to the use of thrombolytics to treat ischaemic stroke. Inflammation-dependent neurodegeneration occurs over a prolonged period after stroke. Melatonin has been shown to have anti-inflammatory, anti-oxidant, and neuroprotective effects after cerebral ischaemia reperfusion (IR) injury. This thesis focuses on the mechanisms of neuroprotection conferred by melatonin application after cerebral IR injury. Transient (2 hours) cerebral IR was achieved using the filament insertion model of middle cerebral artery occlusion (MCAO). The animals were first dosed (5 mg/kg i.p.) 1 hour after MCAO and two further doses administered over the next 48 hours. Melatonin administration was confirmed to reduce infarct size and be non-toxic. Immunohistochemistry was used to localise endogenous melatonin and its receptors (MT1 and MT2) in cerebral IR in MCAO and control animals. The majority of cells expressing melatonin and its receptors were found within the hypothalamus. The presence of melatonin and its receptors in the blood vessels, may suggest a role for melatonin in controlling immune cell infiltration after cerebral IR. Following MCAO, cellular immunoreactivity to melatonin receptor antibodies increased. Within the infarct, infiltrating inflammatory cells expressed melatonin and MT2 receptor. Following MCAO, major inducible enzymes such as nitric oxide synthase (iNOS), and cyclooxygenase (COX-II) were stimulated. Melatonin administration resulted in a significant decrease in iNOS activity as well as total NOS activity and a consequent decrease in nitrite levels. Melatonin administration also attenuated both the MCAO-induced increase in COX expression and activity. HT-1080 human fibrosarcoma fibroblasts was utilised to probe the effects of melatonin on
arginase enzymes. Melatonin treatment decreased cell viability at high concentrations, and this effect was attributed to its pro-oxidant effect present at these concentrations in cancer cell lines. The pro-oxidant effect was associated with increased total NOS activity. On the other hand, both
arginase II expression and activity were increased with higher concentrations of melatonin treatment. These results highlighted the possibility that melatonin may be able to stimulate eNOS and
arginase enzymes, both of which are beneficial after cerebral IR. Inflammation occurring after cerebral IR has been linked closely to mitochondria-driven apoptosis. Increased oxidative stress was seen as indicated by inhibition of aconitase enzyme activity after MCAO. Consequently, most of the electron transport chain complexes measured were significantly impaired. Melatonin administration led to protection of electron transport chain complexes, thus providing evidence of mitochondrial protection after MCAO. In conclusion, this thesis has highlighted the multifaceted…
Advisors/Committee Members: Giles, Greg (advisor).
Subjects/Keywords: Melatonin;
MCAO;
L-arginine metabolism;
Nitric oxide synthase;
Arginase;
Mitochondria
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APA (6th Edition):
Nair, S. M. (2011). The Mechanisms of Neuroprotection by Melatonin in Cerebral Ischaemia Reperfusion
. (Doctoral Dissertation). University of Otago. Retrieved from http://hdl.handle.net/10523/1956
Chicago Manual of Style (16th Edition):
Nair, Shiva Madhwan. “The Mechanisms of Neuroprotection by Melatonin in Cerebral Ischaemia Reperfusion
.” 2011. Doctoral Dissertation, University of Otago. Accessed January 19, 2021.
http://hdl.handle.net/10523/1956.
MLA Handbook (7th Edition):
Nair, Shiva Madhwan. “The Mechanisms of Neuroprotection by Melatonin in Cerebral Ischaemia Reperfusion
.” 2011. Web. 19 Jan 2021.
Vancouver:
Nair SM. The Mechanisms of Neuroprotection by Melatonin in Cerebral Ischaemia Reperfusion
. [Internet] [Doctoral dissertation]. University of Otago; 2011. [cited 2021 Jan 19].
Available from: http://hdl.handle.net/10523/1956.
Council of Science Editors:
Nair SM. The Mechanisms of Neuroprotection by Melatonin in Cerebral Ischaemia Reperfusion
. [Doctoral Dissertation]. University of Otago; 2011. Available from: http://hdl.handle.net/10523/1956
University of Manchester
14.
Bowcutt, Rowann.
Dendritic cells as a biomarker for gut pathology.
Degree: PhD, 2012, University of Manchester
URL: https://www.research.manchester.ac.uk/portal/en/theses/dendritic-cells-as-a-biomarker-for-gut-pathology(33ff2fed-3955-4401-bd01-6df1f8397642).html
;
http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.555576
► Trichuris trichiura (T. Trichiura) is a large-intestinal dwelling nematode that affects over 1 billion people world-wide and thus has large global significance. Much of our…
(more)
▼ Trichuris trichiura (T. Trichiura) is a large-intestinal dwelling nematode that affects over 1 billion people world-wide and thus has large global significance. Much of our understanding of T. trichiura infection comes from the study of the mouse model Trichuris muris (T. muris). However, how the immune system is initiated in response to helminth threat and how inflammation and pathology are resolved in T. muris infection still remain to be addressed. Here, I have attempted to provide insight into these questions. Previous work has shown resistance to T. muris infection is associated with the rapid recruitment of dendritic cells (DCs) to the colonic epithelium via epithelial production of CCL5 and CCL20. However, the epithelial-parasite interaction that drives chemokine production is not known. Pattern recognition receptor (PRRS) are critical mediators of pathogen recognition but there is no known (PRR) specific for T. muris. Here, we address the role of the cytosolic pattern recognition receptor Nod2, the location of which within the crypts correlates with the T. muris niche. In WT mice, in response to infection, there was a rapid influx of CD103+CD11c+ DCs into the colonic epithelium, whereas, this recruitment was impaired in Nod2 /- animals. In vitro and in vivo experiments confirmed the impairment in DC recruitment in Nod2-/- mice was attributable to the epithelial compartment. Subsequent work revealed decreased production of epithelial chemokines in the absence of functional Nod2. Thus, we have shown a novel role for Nod2 in the initiation the immune response to T. muris. We next addressed how pathology is regulated during T. muris infection. Firstly we investigated the role of arginase and Arg1-expressing macrophages in regulating pathology. My data showed that, unlike other gastrointestinal helminths, arginase and Arg1-expressing macrophages are not essential for resistance to T. muris or effective resolution of helminth-induced inflammation. I also addressed the role of DCs in the resolution of infection. DCs can regulate immune responses via the anti-inflammatory cytokine IL-10 and induction of regulatory T cells (Treg). I used an IL 10flox/floxCD11cCre transgenic model in which mice have DCs that cannot make IL-10. I found no role for CD11c+ cell mediated IL-10 production in the regulation of pathogen induced pathology in chronic T. muris infection. In summary I have been able to identify factors in the initiation of immunity to T. muris namely epithelial expression of Nod2. However, as arginase, Arg1-expressing macrophages and DC derived IL-10 appeared to play a redundant role in T. muris infection, the question as to how infection induced inflammation is resolved remains elusive.
Subjects/Keywords: 616.9; Dendritic Cell; Nod2; Macrophage; Arginase; Trichuris muris
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APA (6th Edition):
Bowcutt, R. (2012). Dendritic cells as a biomarker for gut pathology. (Doctoral Dissertation). University of Manchester. Retrieved from https://www.research.manchester.ac.uk/portal/en/theses/dendritic-cells-as-a-biomarker-for-gut-pathology(33ff2fed-3955-4401-bd01-6df1f8397642).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.555576
Chicago Manual of Style (16th Edition):
Bowcutt, Rowann. “Dendritic cells as a biomarker for gut pathology.” 2012. Doctoral Dissertation, University of Manchester. Accessed January 19, 2021.
https://www.research.manchester.ac.uk/portal/en/theses/dendritic-cells-as-a-biomarker-for-gut-pathology(33ff2fed-3955-4401-bd01-6df1f8397642).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.555576.
MLA Handbook (7th Edition):
Bowcutt, Rowann. “Dendritic cells as a biomarker for gut pathology.” 2012. Web. 19 Jan 2021.
Vancouver:
Bowcutt R. Dendritic cells as a biomarker for gut pathology. [Internet] [Doctoral dissertation]. University of Manchester; 2012. [cited 2021 Jan 19].
Available from: https://www.research.manchester.ac.uk/portal/en/theses/dendritic-cells-as-a-biomarker-for-gut-pathology(33ff2fed-3955-4401-bd01-6df1f8397642).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.555576.
Council of Science Editors:
Bowcutt R. Dendritic cells as a biomarker for gut pathology. [Doctoral Dissertation]. University of Manchester; 2012. Available from: https://www.research.manchester.ac.uk/portal/en/theses/dendritic-cells-as-a-biomarker-for-gut-pathology(33ff2fed-3955-4401-bd01-6df1f8397642).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.555576
Portland State University
15.
O'Malley, Karen Laurel.
Characteristics of Arginase from the Terebellid Polychaete Pista Pacifica Berkeley.
Degree: MS(M.S.) in Biology, Biology, 1973, Portland State University
URL: https://pdxscholar.library.pdx.edu/open_access_etds/1660
► Arginase has been found to occur in the tentacles, gut, and body wall of Pista pacifica Berkeley_ Partially purified arginase from the intestine has…
(more)
▼ Arginase has been found to occur in the tentacles, gut, and body wall of Pista pacifica Berkeley_ Partially purified arginase from the intestine has a molecular weight of 200,000, a Km of about 155 mM, an arginase/canavanase ratio of 22, a pH optimum of 10.5, and a temperature optimum of 60°C. In addition, P. pacifica arginase is competitively inhibited by ornithine but is not inhibited by high arginine concentrations, nor by sulfhydryl reagents. The enzyme is not stimulated by exogenous manganese and breaks down into an active subunit under harsh treatment. The subunit has a KID of about 118 mM and is also unaffected by exogenous manganese.
Polychaete arginase shows most of the properties characteristic of arginases from other animal and plant species. However, none of the characteristics observed to date can be correlated with a particular mode of nitrotelism.
Subjects/Keywords: Pista pacifica Berkeley; Arginase; Biology
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APA (6th Edition):
O'Malley, K. L. (1973). Characteristics of Arginase from the Terebellid Polychaete Pista Pacifica Berkeley. (Masters Thesis). Portland State University. Retrieved from https://pdxscholar.library.pdx.edu/open_access_etds/1660
Chicago Manual of Style (16th Edition):
O'Malley, Karen Laurel. “Characteristics of Arginase from the Terebellid Polychaete Pista Pacifica Berkeley.” 1973. Masters Thesis, Portland State University. Accessed January 19, 2021.
https://pdxscholar.library.pdx.edu/open_access_etds/1660.
MLA Handbook (7th Edition):
O'Malley, Karen Laurel. “Characteristics of Arginase from the Terebellid Polychaete Pista Pacifica Berkeley.” 1973. Web. 19 Jan 2021.
Vancouver:
O'Malley KL. Characteristics of Arginase from the Terebellid Polychaete Pista Pacifica Berkeley. [Internet] [Masters thesis]. Portland State University; 1973. [cited 2021 Jan 19].
Available from: https://pdxscholar.library.pdx.edu/open_access_etds/1660.
Council of Science Editors:
O'Malley KL. Characteristics of Arginase from the Terebellid Polychaete Pista Pacifica Berkeley. [Masters Thesis]. Portland State University; 1973. Available from: https://pdxscholar.library.pdx.edu/open_access_etds/1660
Duke University
16.
Kan, Matthew.
Immunity and Arginine Deprivation in Alzheimer's Disease
.
Degree: 2015, Duke University
URL: http://hdl.handle.net/10161/12097
► The pathogenesis of Alzheimer’s disease (AD) is a critical unsolved question, and while recent studies have demonstrated a strong association between altered brain immune…
(more)
▼ The pathogenesis of Alzheimer’s disease (AD) is a critical unsolved question, and while recent studies have demonstrated a strong association between altered brain immune responses and disease progression, the mechanistic cause of neuronal dysfunction and death is unknown. We have previously described the unique CVN-AD mouse model of AD, in which immune-mediated nitric oxide is lowered to mimic human levels, resulting in a mouse model that demonstrates the cardinal features of AD, including amyloid deposition, hyperphosphorylated and aggregated tau, behavioral changes and age-dependent hippocampal neuronal loss. Using this mouse model, we studied longitudinal changes in brain immunity in relation to neuronal loss and, contrary to the predominant view that AD pathology is driven by pro-inflammatory factors, we find that the pathology in CVN-AD mice is driven by local immune suppression. Areas of hippocampal neuronal death are associated with the presence of immunosuppressive CD11c+ microglia and extracellular
arginase, resulting in arginine catabolism and reduced levels of total brain arginine. Pharmacologic disruption of the arginine utilization pathway by an inhibitor of
arginase and ornithine decarboxylase protected the mice from AD-like pathology and significantly decreased CD11c expression. Our findings strongly implicate local immune-mediated amino acid catabolism as a novel and potentially critical mechanism mediating the age-dependent and regional loss of neurons in humans with AD. There is a large interest in identifying, lineage tracing, and determining the physiologic roles of monophagocytes in Alzheimer’s disease. While Cx3cr1 knock-in fluorescent reporting and Cre expressing mice have been critical for studying neuroimmunology, mice that are homozygous null or hemizygous for CX3CR1 have perturbed neural development and immune responses. There is, therefore, a need for similar tools in which mice are CX3CR1+/+. Here, we describe a mouse where Cre is driven by the Cx3cr1 promoter on a bacterial artificial chromosome (BAC) transgene (Cx3cr1-CreBT) and the Cx3cr1 locus is unperturbed. Similarly to Cx3cr1-Cre knock-in mice, these mice express Cre in Ly6C-, but not Ly6C+, monocytes and tissue macrophages, including microglia. These mice represent a novel tool that maintains the Cx3cr1 locus while allowing for selective gene targeting in monocytes and tissue macrophages. The study of immunity in Alzheimer’s requires the ability to identify and quantify specific immune cell subsets by flow cytometry. While it is possible to identify lymphocyte subsets based on cell lineage-specific markers, the lack of such markers in brain myeloid cell subsets has prevented the study of monocytes, macrophages and dendritic cells. By improving on tissue homogenization, we present a comprehensive protocol for flow cytometric analysis, that allows for the identification of several cell types that have not been previously identified by flow cytometry. These cell types include F4/80hi macrophages, which may be…
Advisors/Committee Members: Gunn, Michael D (advisor), Colton, Carol A (advisor).
Subjects/Keywords: Immunology;
Neurosciences;
Medicine;
Alzheimer's;
arginase;
arginine;
Cx3cr1;
immunosuppression;
microglia
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APA (6th Edition):
Kan, M. (2015). Immunity and Arginine Deprivation in Alzheimer's Disease
. (Thesis). Duke University. Retrieved from http://hdl.handle.net/10161/12097
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Kan, Matthew. “Immunity and Arginine Deprivation in Alzheimer's Disease
.” 2015. Thesis, Duke University. Accessed January 19, 2021.
http://hdl.handle.net/10161/12097.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Kan, Matthew. “Immunity and Arginine Deprivation in Alzheimer's Disease
.” 2015. Web. 19 Jan 2021.
Vancouver:
Kan M. Immunity and Arginine Deprivation in Alzheimer's Disease
. [Internet] [Thesis]. Duke University; 2015. [cited 2021 Jan 19].
Available from: http://hdl.handle.net/10161/12097.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Kan M. Immunity and Arginine Deprivation in Alzheimer's Disease
. [Thesis]. Duke University; 2015. Available from: http://hdl.handle.net/10161/12097
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Louisiana State University
17.
Guan, Ying.
The Role of Polyamines in Osmotic Stress Tolerance in Gulf Killifish Fundulus Grandis.
Degree: PhD, 2013, Louisiana State University
URL: etd-06272013-193138
;
https://digitalcommons.lsu.edu/gradschool_dissertations/1123
► Polyamines are a diverse class of aliphatic molecules that are highly polycationic at physiological intracellular pH. Polyamine levels are regulated by the coordinated actions of…
(more)
▼ Polyamines are a diverse class of aliphatic molecules that are highly polycationic at physiological intracellular pH. Polyamine levels are regulated by the coordinated actions of arginase (Arg), ornithine decarboxylase (Odc), and polyamine oxidase (Pao). Although little is known of their functions in fish, polyamines have been implicated in diverse physiological processes, including RNA transcription, cell growth, cell proliferation, and cell apoptosis. The main objective of this study was to describe the transcription and enzymatic activities of key enzymes for polyamine metabolism, to measure polyamine levels, and to assess putative roles of polyamines in the gills of the Gulf killifish (Fundulus grandis) during hypoosmotic challenge. In addition, the influence of irreversible inhibition of Odc by alpha-DL-difluoromethylornithine (DFMO) was assessed in the gills. Furthermore, the transcription and enzymatic activities of Arg, Odc and Pao was assessed in other tissues such as intestine and liver during hypoosmoitc challenges. Adult F. grandis were reared in 5 ppt and acutely transferred to 5, 2, 1, 0.5, and 0.1 ppt water, and gills were sampled at 6 h, 1 d, 3 d, and 7 d post-transfer. Results showed that arg II and odc mRNA levels were highly upregulated in the gills during the first few days post-transfer to hypoosmotic water. Hypoosmotic exposure also produced increases in gill Odc activity and in the concentrations of putrescine, spermidine, and spermine. DFMO application inhibited Odc activity and reduced polyamine levels after hypoosmotic exposure (0.1 ppt). Although gill putrescine levels remained elevated throughout the first 7 d post transfer to 0.1 ppt, the concentrations of spermidine and spermine decreased over time. The ratio of putrescine level over the sum levels of spermidine and spermine increased after 0.1 ppt exposure at 1 d and beyond. Concomitant with freshwater acclimation, an increase in Pao activity suggested that polyamine catabolism was upregulated in the gills. The phenotype of mitochondrion-rich cells (MRCs) in the gill epithelium shifted from a seawater type to a freshwater type following transfer to 0.1 ppt water in correlation with the increase in mRNA levels of arg II and odc in MRCs. In addition, the isolated opercular epithelium pretreated with spermidine had a lower active Cl- secretion rate and membrane conductance following symmetrical hypotonic exposure. Transcription and enzymatic activities of Arg II, Odc, and Pao were upregulated in the intestine and liver during hypoosmotic exposure, suggesting that polyamine levels are regulated in multiple tissues of the killifish. The putative roles of polyamines include inducing cell apoptosis by increasing caspase-3 activity, stimulating cell proliferation by increasing the levels of c-fos and c-myc mRNA levels, and inducing cell swelling via the modulation of Cl- secretion in the gills following hypoosmotic challenges. In summary, fish gill and intestine of killifish transferred to fresh water underwent dramatic physiological and…
Subjects/Keywords: ornithine decarboxylase; arginase; osmoregulation; polyamine; killifish; gill epithelium; gill remodeling
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APA (6th Edition):
Guan, Y. (2013). The Role of Polyamines in Osmotic Stress Tolerance in Gulf Killifish Fundulus Grandis. (Doctoral Dissertation). Louisiana State University. Retrieved from etd-06272013-193138 ; https://digitalcommons.lsu.edu/gradschool_dissertations/1123
Chicago Manual of Style (16th Edition):
Guan, Ying. “The Role of Polyamines in Osmotic Stress Tolerance in Gulf Killifish Fundulus Grandis.” 2013. Doctoral Dissertation, Louisiana State University. Accessed January 19, 2021.
etd-06272013-193138 ; https://digitalcommons.lsu.edu/gradschool_dissertations/1123.
MLA Handbook (7th Edition):
Guan, Ying. “The Role of Polyamines in Osmotic Stress Tolerance in Gulf Killifish Fundulus Grandis.” 2013. Web. 19 Jan 2021.
Vancouver:
Guan Y. The Role of Polyamines in Osmotic Stress Tolerance in Gulf Killifish Fundulus Grandis. [Internet] [Doctoral dissertation]. Louisiana State University; 2013. [cited 2021 Jan 19].
Available from: etd-06272013-193138 ; https://digitalcommons.lsu.edu/gradschool_dissertations/1123.
Council of Science Editors:
Guan Y. The Role of Polyamines in Osmotic Stress Tolerance in Gulf Killifish Fundulus Grandis. [Doctoral Dissertation]. Louisiana State University; 2013. Available from: etd-06272013-193138 ; https://digitalcommons.lsu.edu/gradschool_dissertations/1123
Georgia State University
18.
Abdelbaky Abdelaal, Ahmed.
Induction of arginase-1 in MDSC requires exposure to CD3/CD28 activated T cells.
Degree: MS, Biology, 2017, Georgia State University
URL: https://scholarworks.gsu.edu/biology_theses/76
► Tumor-induced myeloid derived suppressive cells (MDSC) have been reported to inhibit T cell responses. In our study MDSC isolated from tumor bearing mice showed…
(more)
▼ Tumor-induced myeloid derived suppressive cells (MDSC) have been reported to inhibit T cell responses. In our study MDSC isolated from tumor bearing mice showed potent inhibition of T-cell proliferation. However, surprisingly we observed that freshly isolated MDSC from the bone marrow of tumor bearing mice do not constitutively express
arginase-1 until after exposure to T-cell proliferation. The aim of this study is to determine the mechanism by which
arginase-1 is induced in MDSC following exposure to a proliferative T cell environment. We showed that treatment of MDSC with culture supernatant isolated from T cells activated with CD3/CD28 antibodies successfully induced
arginase-1 expression and this process is independent of IL-10 and IFNγ. This suggested that
arginase-1 induction in MDSC can occur independently of cell-cell contact. Interestingly IL-2, ConA or PMA activated T-cell supernatant as well as supernatant from multiple cancer cell lines failed to induce
arginase-1 in MDSC. We also showed that M-MDSC expressed higher levels of
arginase-1 than G-MDSC after co-culture with CD3/CD28 activated T cells as well as its supernatant. In addition, other bone marrow cells have shown the potential to express
arginase-1 following exposure to the same conditions. For example, we observed that healthy Ly6C+ monocytes but not mature granulocytes successfully expressed
arginase-1.These data demonstrated that T cells activated through stimulation of TCR but not other means of activation induced
arginase-1 enzyme expression.
Advisors/Committee Members: Yuan Liu, William Walthall, Deborah Baro.
Subjects/Keywords: Myeloid derived suppressor cells; MDSC; CD3; T cells; Arginase-1
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APA (6th Edition):
Abdelbaky Abdelaal, A. (2017). Induction of arginase-1 in MDSC requires exposure to CD3/CD28 activated T cells. (Thesis). Georgia State University. Retrieved from https://scholarworks.gsu.edu/biology_theses/76
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Abdelbaky Abdelaal, Ahmed. “Induction of arginase-1 in MDSC requires exposure to CD3/CD28 activated T cells.” 2017. Thesis, Georgia State University. Accessed January 19, 2021.
https://scholarworks.gsu.edu/biology_theses/76.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Abdelbaky Abdelaal, Ahmed. “Induction of arginase-1 in MDSC requires exposure to CD3/CD28 activated T cells.” 2017. Web. 19 Jan 2021.
Vancouver:
Abdelbaky Abdelaal A. Induction of arginase-1 in MDSC requires exposure to CD3/CD28 activated T cells. [Internet] [Thesis]. Georgia State University; 2017. [cited 2021 Jan 19].
Available from: https://scholarworks.gsu.edu/biology_theses/76.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Abdelbaky Abdelaal A. Induction of arginase-1 in MDSC requires exposure to CD3/CD28 activated T cells. [Thesis]. Georgia State University; 2017. Available from: https://scholarworks.gsu.edu/biology_theses/76
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Pennsylvania
19.
Hai, Yang.
Structure and Function of Metallohydrolases in the Arginase-Deacetylase Family.
Degree: 2016, University of Pennsylvania
URL: https://repository.upenn.edu/edissertations/1753
► Arginases and deacetylases are metallohydrolases that catalyze two distinct chemical transformations. The arginases catalyze the hydrolysis of the guanidinium group of arginine by using a…
(more)
▼ Arginases and deacetylases are metallohydrolases that catalyze two distinct chemical transformations. The arginases catalyze the hydrolysis of the guanidinium group of arginine by using a hydroxide ion bridging the binuclear manganese cluster (Mn2+A-Mn2+B) for nucleophilic attack. The deacetylases catalyze the hydrolysis of amide bonds by using a mononuclear Zn2+-ion activated water molecule as the nucleophile. Despite the diverse functions, metallohydrolases of the arginase-deacetylase superfamily share the same characteristic α/β hydrolase core fold and a conserved metal binding site (the Mn2+B site in arginase corresponds to the catalytic Zn2+ site in deacetylase) which is essential for catalysis in both enzymes. We report crystal structure of formiminoglutamase from the parasitic protozoan Trypanosoma cruzi and confirm that formiminoglutamase is a Mn2+-requiring hydrolase that belongs to the arginase-deacetylase superfamily. We also report the crystal structure of an arginase-like protein from Trypanosoma brucei (TbARG) with unknown function. Although its biological role remains enigmatic, the evolutionarily more conserved Mn2+B site can be readily restored in TbARG through side-directed mutagenesis. We also report crystal structure of an arginase from the parasite Schistosoma mansoni (SmARG). Structural comparison of SmARG complexed with second-generation arginase inhibitors (α,α-disubstituted boronic acid inhibitors) with another parasitic arginase from Leishmania mexicana and human arginases reveal interesting differences in the binding modes of the additional α-substituents.
Reversible lysine acetylation rivals phosphorylation in the regulation of protein structure and function, and inhibition of the “eraser” histone deacetylase (HDAC) is a validated approach for cancer chemotherapy. HDAC6, the sole HDAC that harbors a full duplication of catalytic domain (CD1 and CD2), is a cytosolic lysine deacetylase known to deacetylate α-tubulin, heat-shock protein 90, etc. Here, we report HDAC6 structures that provide new insights about mechanism, catalysis, and inhibitor binding. In light of biochemical studies, we reveal a “gate constriction” mechanism responsible for the strict substrate specificity of CD1 versus broad substrate specificity of CD2. Analysis of other isozymes indicates that the closest relative HDAC10 contains an alternative gatekeeper that favors catalysis with acetylpolyamines. Indeed, we provide structural evidence that HDAC10 is the long-sought mammalian N8-acetylspermidine deacetylase whose identity has remained elusive for 30 years.
Subjects/Keywords: Arginase; Crystallography; Histone deacetylase; Metallohydrolase; polyamine deacetylase; Biochemistry
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APA (6th Edition):
Hai, Y. (2016). Structure and Function of Metallohydrolases in the Arginase-Deacetylase Family. (Thesis). University of Pennsylvania. Retrieved from https://repository.upenn.edu/edissertations/1753
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Hai, Yang. “Structure and Function of Metallohydrolases in the Arginase-Deacetylase Family.” 2016. Thesis, University of Pennsylvania. Accessed January 19, 2021.
https://repository.upenn.edu/edissertations/1753.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Hai, Yang. “Structure and Function of Metallohydrolases in the Arginase-Deacetylase Family.” 2016. Web. 19 Jan 2021.
Vancouver:
Hai Y. Structure and Function of Metallohydrolases in the Arginase-Deacetylase Family. [Internet] [Thesis]. University of Pennsylvania; 2016. [cited 2021 Jan 19].
Available from: https://repository.upenn.edu/edissertations/1753.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Hai Y. Structure and Function of Metallohydrolases in the Arginase-Deacetylase Family. [Thesis]. University of Pennsylvania; 2016. Available from: https://repository.upenn.edu/edissertations/1753
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Guelph
20.
Lau, Elisa P. Y.
Pathways of Hydroxyurea Synthesis in the Mitochondria of the Little Skate (Leucoraja erincea).
Degree: MS, Department of Integrative Biology, 2020, University of Guelph
URL: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/21329
► Hydroxyurea (HU) is an anti-proliferative and anti-microbial compound that is widely used in medical therapy but is also found to occur naturally in different phylogenetic…
(more)
▼ Hydroxyurea (HU) is an anti-proliferative and anti-microbial compound that is widely used in medical therapy but is also found to occur naturally in different phylogenetic groups. However, it is still unknown how this compound is endogenously synthesized. I hypothesized that HU is synthesized through either the diversion of hydroxyarginine from the nitric oxide synthase (NOS) pathway by
arginase, or the hydroxylation of urea by cytochrome P450. To test the possible pathways involved in HU synthesis, I incubated isolated Leucoraja erinacea mitochondria with stable isotope labelled arginine and enzymatic inhibitors and determined the activity of mitochondrial
arginase and NOS. While HU was found to be mainly mitochondrial, the inhibition of either enzyme had no effect on the rate of HU production, suggesting that another pathway is involved in HU synthesis. The pathway for HU synthesis is still unknown. However, this study contributes to the known evolutionary timeline for mitochondrial NOS.
Advisors/Committee Members: Ballantyne, Jim S. (advisor).
Subjects/Keywords: hydroxyurea; elasmobranchs; mitochondria; gas chromatography mass spectrophotometry; arginase; nitric oxide synthase
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APA (6th Edition):
Lau, E. P. Y. (2020). Pathways of Hydroxyurea Synthesis in the Mitochondria of the Little Skate (Leucoraja erincea). (Masters Thesis). University of Guelph. Retrieved from https://atrium.lib.uoguelph.ca/xmlui/handle/10214/21329
Chicago Manual of Style (16th Edition):
Lau, Elisa P Y. “Pathways of Hydroxyurea Synthesis in the Mitochondria of the Little Skate (Leucoraja erincea).” 2020. Masters Thesis, University of Guelph. Accessed January 19, 2021.
https://atrium.lib.uoguelph.ca/xmlui/handle/10214/21329.
MLA Handbook (7th Edition):
Lau, Elisa P Y. “Pathways of Hydroxyurea Synthesis in the Mitochondria of the Little Skate (Leucoraja erincea).” 2020. Web. 19 Jan 2021.
Vancouver:
Lau EPY. Pathways of Hydroxyurea Synthesis in the Mitochondria of the Little Skate (Leucoraja erincea). [Internet] [Masters thesis]. University of Guelph; 2020. [cited 2021 Jan 19].
Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/21329.
Council of Science Editors:
Lau EPY. Pathways of Hydroxyurea Synthesis in the Mitochondria of the Little Skate (Leucoraja erincea). [Masters Thesis]. University of Guelph; 2020. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/21329
21.
Rômulo José Soares Bezerra.
Avaliação da modulação das atividades da óxido nítrico sintase (NOS)/arginase como participantes do sistema de defesa de Leishmania amazonensis frente a derivados de compostos mesoiônicos.
Degree: 2008, Fundação Oswaldo Cruz
URL: http://www.bdtd.cict.fiocruz.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=171
► A L-arginina está envolvida na produção de óxido nítrico (NO) e citrulina, mediada pela óxido nítrico sintase (NOS), e L-ornitina e uréia, por atividade da…
(more)
▼ A L-arginina está envolvida na produção de óxido nítrico (NO) e citrulina, mediada pela óxido nítrico sintase (NOS), e L-ornitina e uréia, por atividade da arginase. A regulação de NOS ocorre principalmente em nível transcricional. Trabalhos prévios de nosso grupo demonstram a atividade de NOS em parasitos do gênero Leishmania. A atividade da arginase e sua seqüência genética também foram descritas na literatura para estes parasitos. Em busca de novos alvos intracelulares do parasito para ação de agentes leishmanicidas, foram realizados ensaios com derivados mesiônicos da classe dos 1,3,4-tiadiazóis e das arilsidnonas. Os parasitos foram analisados em ausência/presença de derivados 4- e 3-metoxilados, a fim de verificar o efeito sobre a produção de NO e sobre a atividade de arginase de L. amazonensis. Os resultados indicaram que as drogas testadas reduziram de 70 a 90% a produção NO pelo parasito, e que os mesmos são capazes de diminuir a atividade da NOS solúvel constitutiva purificada de promastigotas e amastigotas axênicas de L. amazonensis. Além disso, ensaios realizados com 4-fenil-5-(4-X-fenil)-1,2,3-tiadiazólio-2-fenilaminida, onde X=H (MIH), NO2 (MI-NO2) ou OCH3 (MI-OCH3) e 3[N-4-X-fenil]-1,2,3-oxadiazólio-5-olato, onde X=H (SID-H), NO2 (SID-NO2) ou OCH3 (SID-OCH3) demonstraram maior eficácia contra amastigotas axênicas, em comparação com o Isetionato de Pentamidina. Os ensaios de citotoxicidade para macrófagos peritoneais murinos demonstraram que tais derivados apresentam baixa toxicidade (~10%). A maioria dos derivados testados não afetou significantemente a atividade da arginase de L. amazonensis.
L-arginine is involved in the production of both nitric oxide (NO) and citrulline, mediated by nitric oxide synthase (NOS), and L-ornithine and urea, by arginase activity. The NOS regulation occurs mainly at the transcriptional level. In previous work we show that parasite of Leishmania genus have a NOS activity. The arginase activity and its gene sequence has also been reported in the literature on Leishmania parasites. In a search for intracellular targets for potential antileishmanicidal agents, we performed assays using aryl sydnone and 1,3,4- thiadiazolium mesoionic compounds. Parasites were assayed in absence/presence of 4- and 3- methoxy mesoionic derivatives in order to verify the effect on NO production and arginase activity in L. amazonensis. The results indicated that the drugs reduce from 70 to 90% of the NO production by the parasite and act on a soluble constitutive nitric oxide synthase purified from L. amazonensis promastigotes and axenic amastigotes. Furthermore, assays with 4-phenyl-5-(4-Xphenyl)- 1,2,3-thiadiazolium-2-phenylaminide, where X=H (MI-H), NO2 (MI-NO2) and OCH3 (MI-OCH3) and 3[N-4-X-phenyl]-1,2,3-oxadiazolium-5-olate, where X=H (SID-H), NO2 (SID-NO2) and OCH3 (SID-OCH3) showed a major efficacy of all compounds against axenic amastigotes, compared to Pentamidine isethionate. Assessment of citotoxicity for peritoneal murine macrophage cultures showed low toxicity (~10%). The…
Advisors/Committee Members: Marcelo da Silva Genestra.
Subjects/Keywords: Thiadiazoles; Nitric Oxide Synthase; Leishmania; Compostos Orgânicos; Tiadiazóis; Óxido Nítrico Sintase; Arginase; Leishmaniose; BIOQUIMICA DOS MICROORGANISMOS; Humanos; Leishmania; Organic Chemicals; Arginase; Leishmaniasis
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APA ·
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APA (6th Edition):
Bezerra, R. J. S. (2008). Avaliação da modulação das atividades da óxido nítrico sintase (NOS)/arginase como participantes do sistema de defesa de Leishmania amazonensis frente a derivados de compostos mesoiônicos. (Thesis). Fundação Oswaldo Cruz. Retrieved from http://www.bdtd.cict.fiocruz.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=171
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Bezerra, Rômulo José Soares. “Avaliação da modulação das atividades da óxido nítrico sintase (NOS)/arginase como participantes do sistema de defesa de Leishmania amazonensis frente a derivados de compostos mesoiônicos.” 2008. Thesis, Fundação Oswaldo Cruz. Accessed January 19, 2021.
http://www.bdtd.cict.fiocruz.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=171.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Bezerra, Rômulo José Soares. “Avaliação da modulação das atividades da óxido nítrico sintase (NOS)/arginase como participantes do sistema de defesa de Leishmania amazonensis frente a derivados de compostos mesoiônicos.” 2008. Web. 19 Jan 2021.
Vancouver:
Bezerra RJS. Avaliação da modulação das atividades da óxido nítrico sintase (NOS)/arginase como participantes do sistema de defesa de Leishmania amazonensis frente a derivados de compostos mesoiônicos. [Internet] [Thesis]. Fundação Oswaldo Cruz; 2008. [cited 2021 Jan 19].
Available from: http://www.bdtd.cict.fiocruz.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=171.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Bezerra RJS. Avaliação da modulação das atividades da óxido nítrico sintase (NOS)/arginase como participantes do sistema de defesa de Leishmania amazonensis frente a derivados de compostos mesoiônicos. [Thesis]. Fundação Oswaldo Cruz; 2008. Available from: http://www.bdtd.cict.fiocruz.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=171
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
22.
Rosangela Almeida Victor.
Resposta metabólica aeróbia, anaeróbia e argininolítica do bagre Genidens Genidens, (Curvier, 1829), presentes nos estuários do município de Ubatuba.
Degree: 2009, Universidade de Taubaté
URL: http://www.bdtd.unitau.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=183
► A resposta metabólica do bagre Genidens genidens de três estuários (rios Grande, Indaiá e Escuro) do município de Ubatuba SP foi estudada comparativamente em relação…
(more)
▼ A resposta metabólica do bagre Genidens genidens de três estuários (rios Grande, Indaiá e Escuro) do município de Ubatuba SP foi estudada comparativamente em relação ao impacto da atividade humana na região. Encéfalo, fígado e músculo epaxial foram utilizados para determinar a atividade específica das enzimas lactato desidrogenase (LDH), malato desidrogenase (MDH) e arginase, como prováveis marcadoras desse impacto. As diferenças entre os níveis teciduais de LDH dos bagres coletados nos três estuários não foram significativamente diferentes, provavelmente devido a sua capacidade adaptativa, associada à sazonalidade das concentrações de oxigênio nas águas estuarinas. Os níveis de arginase hepática e muscular dos bagres coletados nos estuários dos rios Escuro e Indaiá, respectivamente, foram diferentes em relação aos dois outros estuários. Comportamento semelhante a esse foi constatado em relação aos níveis da enzima MDH, marcadora do potencial gerador de ATP desses tecidos. Nesse sentido, os níveis de arginase e MDH do fígado e músculo epaxial podem estar refletindo o esforço adaptativo metabólico desse bagre às condições ambientais dessas regiões estuarinas.
The metabolic response of catfish Genidens genidens of the three estuaries (Grande, Indaia and Escuro rivers) in the municipality of Ubatuba - SP was studied in comparison to the impact of human activity in the region. Brain, liver and epaxial muscle were used to determine the specific activity of the enzymes lactate dehydrogenase (LDH), malate dehydrogenase (MDH) and arginase, as probable markers of that impact. The differences between the tissues levels of LDH of the catfishes collected in the three estuaries were not significantly different, probably due to its adaptive capacity, associated with the seasonality of the oxygen concentrations in estuarine waters. Arginase levels of liver and muscle of the catfishes collected in estuaries of rivers Escuro and Indaia, respectively, were different in the relation to the other two estuaries. Behavior was similar to that observed in the levels of the enzyme MDH, marker of the potential generator of ATP of those tissues. Accordingly, the levels of arginase and MDH of the liver and epaxial muscle might reflect the effort of that adaptive metabolic catfish to environmental conditions such estuarine areas.
Advisors/Committee Members: Wilma Pereira Bastos-Ramos, Valter José Cobo, Edson Rodrigues.
Subjects/Keywords: Ubatuba; estuário; bagre; Genidens genidens; metabolismo energético; biomarcadores; arginase; lactato desidrogenase; malato desidrogenase; Ubatuba; estuary; catfish; Genidens genidens; energy metabolism; biomarkers; arginase; lactate dehydrogenase; malate dehydrogenase; CONSERVACAO DAS ESPECIES ANIMAIS
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APA ·
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CSE |
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to Zotero / EndNote / Reference
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APA (6th Edition):
Victor, R. A. (2009). Resposta metabólica aeróbia, anaeróbia e argininolítica do bagre Genidens Genidens, (Curvier, 1829), presentes nos estuários do município de Ubatuba. (Thesis). Universidade de Taubaté. Retrieved from http://www.bdtd.unitau.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=183
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Victor, Rosangela Almeida. “Resposta metabólica aeróbia, anaeróbia e argininolítica do bagre Genidens Genidens, (Curvier, 1829), presentes nos estuários do município de Ubatuba.” 2009. Thesis, Universidade de Taubaté. Accessed January 19, 2021.
http://www.bdtd.unitau.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=183.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Victor, Rosangela Almeida. “Resposta metabólica aeróbia, anaeróbia e argininolítica do bagre Genidens Genidens, (Curvier, 1829), presentes nos estuários do município de Ubatuba.” 2009. Web. 19 Jan 2021.
Vancouver:
Victor RA. Resposta metabólica aeróbia, anaeróbia e argininolítica do bagre Genidens Genidens, (Curvier, 1829), presentes nos estuários do município de Ubatuba. [Internet] [Thesis]. Universidade de Taubaté; 2009. [cited 2021 Jan 19].
Available from: http://www.bdtd.unitau.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=183.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Victor RA. Resposta metabólica aeróbia, anaeróbia e argininolítica do bagre Genidens Genidens, (Curvier, 1829), presentes nos estuários do município de Ubatuba. [Thesis]. Universidade de Taubaté; 2009. Available from: http://www.bdtd.unitau.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=183
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
23.
Rosetti, Andreza da Silva.
Bordetella pertussis: participação da arginase, TGF-b e TLR4 no controle da síntese de óxido nítrico em macrófagos derivados de medula óssea murina.
Degree: Mestrado, Biotecnologia, 2009, University of São Paulo
URL: http://www.teses.usp.br/teses/disponiveis/87/87131/tde-15032010-164402/
;
► Bordetella pertussis e Bordetella parapertussis são os principais agentes causadores da coqueluche no homem. O óxido nítrico é fundamental para o controle de diversos processos…
(more)
▼ Bordetella pertussis e Bordetella parapertussis são os principais agentes causadores da coqueluche no homem. O óxido nítrico é fundamental para o controle de diversos processos fisiopatológicos. Neste trabalho analisamos sinais moleculares envolvidos na produção de NO em macrófagos derivados de medula óssea murina (BMDMO) infectadas por Bpertussis e Bparapertussis. Nossos resultados mostraram que BMDMO de C57BL/6 estimulados com Bpertussis não sintetizaram níveis significativos de nitrito, ao contrário da infecção com Bparapertussis. BMDMO de C57BL/6 infectados por Bpertussis e Bparapertussis produziram níveis elevados de arginase e de TGFb e esta produção foi dependente de TLR4, porém a produção de NO pelos BMDMO de C3H/HeJ infectados com Bparapertussis foi independente deste receptor. A adição exógena de PT em BMDMO infectados com Bparapertussis reduziu a quantidade de NO sintetizada. Concluímos que TGFb e arginase contribuem para o controle da produção de NO durante a infecção in vitro de BMDMO com Bpertussis e este mecanismo depende de LPS envolvendo TLR4 e PT.
Bordetella pertussis and Bordetella parapertussis are the main etiologic causes of human whooping cough. Nitric oxide (NO) is crucial for several physiopathologic events. Herein we analyzed the molecular signals required for NO production by murine bone marrow-derived macrophages (BMDM) infected with Bpertussis or Bparapertussis. Our data show that BMDM obtained from C57Bl/6 mice was not able to produce measurable levels of nitrite when stimulated with Bpertussis while infection of these cells with Bparapertussis induced high levels of nitrite. Arginase and TLR4-dependent TGF-b were produced in response to infection with either Bpertussis or Bparapertussis. NO production by BMDM obtained from C3H/HeJ mice occurred after Bparapertussis infection in the absence of TLR4. Addition of pertussis toxin to the C57Bl/6 BMDM cultures infected with Bparapertussis decreased NO levels. In conclusion, TGF-b and arginase play a role controlling NO production by BMDM during in vitro infection by Bpertussis. This effect depends on the presence of LPS-TLR4 and PT signaling pathways.
Advisors/Committee Members: Abrahao Neto, Jose, Borges, Monamaris Marques.
Subjects/Keywords: B. pertussis; B. pertussis; Anaerobic bacteria gram-negative; Arginase; Arginase; Bactérias anaeróbicas gram-negativas; Biotechnology; Biotecnologia; Immune regulation; Macrófagos; Macrophage; Nitric oxide; Óxido nítrico; Regulação imune; TGF-b; TGF-b
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Rosetti, A. d. S. (2009). Bordetella pertussis: participação da arginase, TGF-b e TLR4 no controle da síntese de óxido nítrico em macrófagos derivados de medula óssea murina. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/87/87131/tde-15032010-164402/ ;
Chicago Manual of Style (16th Edition):
Rosetti, Andreza da Silva. “Bordetella pertussis: participação da arginase, TGF-b e TLR4 no controle da síntese de óxido nítrico em macrófagos derivados de medula óssea murina.” 2009. Masters Thesis, University of São Paulo. Accessed January 19, 2021.
http://www.teses.usp.br/teses/disponiveis/87/87131/tde-15032010-164402/ ;.
MLA Handbook (7th Edition):
Rosetti, Andreza da Silva. “Bordetella pertussis: participação da arginase, TGF-b e TLR4 no controle da síntese de óxido nítrico em macrófagos derivados de medula óssea murina.” 2009. Web. 19 Jan 2021.
Vancouver:
Rosetti AdS. Bordetella pertussis: participação da arginase, TGF-b e TLR4 no controle da síntese de óxido nítrico em macrófagos derivados de medula óssea murina. [Internet] [Masters thesis]. University of São Paulo; 2009. [cited 2021 Jan 19].
Available from: http://www.teses.usp.br/teses/disponiveis/87/87131/tde-15032010-164402/ ;.
Council of Science Editors:
Rosetti AdS. Bordetella pertussis: participação da arginase, TGF-b e TLR4 no controle da síntese de óxido nítrico em macrófagos derivados de medula óssea murina. [Masters Thesis]. University of São Paulo; 2009. Available from: http://www.teses.usp.br/teses/disponiveis/87/87131/tde-15032010-164402/ ;
Universidade do Estado do Rio de Janeiro
24.
Monique Bandeira Moss.
Mecanismos moleculares e celulares envolvidos na modulação da via L-arginina-óxido nítrico em hipertensão e insuficiência renal crônica.
Degree: PhD, 2010, Universidade do Estado do Rio de Janeiro
URL: http://www.bdtd.uerj.br/tde_busca/arquivo.php?codArquivo=2167
;
► A insuficiência renal crônica (IRC) e a hipertensão arterial sistêmica (HAS) são patologias com alta morbidade e mortalidade, consumindo grandes verbas de saúde pública. A…
(more)
▼ A insuficiência renal crônica (IRC) e a hipertensão arterial sistêmica (HAS) são patologias com alta morbidade e mortalidade, consumindo grandes verbas de saúde pública. A disfunção endotelial presente tanto na IRC, como na hipertensão, contribui para a manutenção de elevada resistência periférica, favorecendo complicações como a aterosclerose. Esta disfunção endotelial é parte de um estado pró-trombótico, levando à ocorrência de eventos cardiovasculares, principal causa de morte nestas patologias. O óxido nítrico (NO) tem um papel importante na modulação da atividade plaquetária. Anormalidades na síntese e/ou inativação do NO são descritas tanto na insuficiência renal crônica como na hipertensão. Estudos prévios demonstraram uma redução do influxo de L-arginina em eritrócitos e plaquetas de pacientes hipertensos e em um modelo animal de hipertensão. Além disso, em IRC, nosso grupo mostrou uma ativação da via L-arginina-NO em plaquetas. O objetivo dessa tese é avaliar a via L-arginina-NO na HAS e em diferentes estágios da IRC, bem como investigar o ciclo da uréia, e a presença de marcadores de estresse nesses pacientes. De acordo com o presente estudo pôde-se verificar que não houve alteração na síntese de NO em eritrócitos na hipertensão, todavia ocorre uma ativação do ciclo da uréia, que pode ser dada pelo aumento do influxo de L-arginina eritrocitário previamente demonstrado. Não foi demonstrada diferença significativa na peroxidação lipídica sistêmica, em plaquetas ou eritrócitos na HAS. Em plaquetas, no entanto, houve uma redução da atividade da NO sintase (NOS), que não foi acompanhada por alteração da expressão das isoformas da NOS, da arginase, da fosfodiesterase 5 (PDE5) ou da guanilato ciclase (GC) solúvel. Essa redução na síntese de NO em plaquetas pode ser explicada por um menor influxo de L-arginina que está presente na hipertensão. Os eritrócitos de pacientes renais crônicos em hemodiálise mostraram um maior influxo de L-arginina associado a um aumento da expressão e da atividade da arginase, não havendo diferença na atividade da NOS. Além disso, apesar e não ter sido mostrado alteração nos marcadores de peroxidação lipídica em eritrócitos e plaquetas, foi detectado um aumento dos mesmos no soro de pacientes com IRC em hemodiálise. Por outro lado, as plaquetas dos mesmos pacientes apresentaram uma maior expressão da eNOS, da iNOS e da GC solúvel, acompanhada de uma redução da atividade da arginase, o que pode justificar a disfunção plaquetária que existe nesses pacientes. Plaquetas de pacientes portadores de IRC em tratamento conservador mostraram um aumento da atividade da NOS associado com maior expressão tanto da iNOS como da eNOS. Curiosamente foram detectados menores concentrações de 35-monofosfato de guanosina cíclica (GMPc), não havendo no entanto, diferença nos padrões de agregação plaquetária induzida por colágeno ou adenosina difosfato (ADP). As descobertas aqui apresentadas certamente contribuirão para uma melhor compreensão da fisiopatologia da HAS e da IRC.
Chronic renal failure (CRF)…
Advisors/Committee Members: Antônio Cláudio Mendes Ribeiro, Tatiana Marlowe Cunha Brunini, Jorge José de Carvalho, Nadja Lima Pinheiro, Miguel Luís Graciano, Sérgio Fernando Ferreira dos Santos.
Subjects/Keywords: Insuficiência rrenal crônica; Hipertensão arterial sistêmica; L-arginina; Óxido nítrico; Arginase; Plaquetas; Eritrócitos; Chronic renal failure; Essential hypertension; Nitric oxide; L-arginine; Arginase; Platelets; Erythrocytes; FARMACOLOGIA CARDIORENAL; Plaquetas (Sangue)
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APA (6th Edition):
Moss, M. B. (2010). Mecanismos moleculares e celulares envolvidos na modulação da via L-arginina-óxido nítrico em hipertensão e insuficiência renal crônica. (Doctoral Dissertation). Universidade do Estado do Rio de Janeiro. Retrieved from http://www.bdtd.uerj.br/tde_busca/arquivo.php?codArquivo=2167 ;
Chicago Manual of Style (16th Edition):
Moss, Monique Bandeira. “Mecanismos moleculares e celulares envolvidos na modulação da via L-arginina-óxido nítrico em hipertensão e insuficiência renal crônica.” 2010. Doctoral Dissertation, Universidade do Estado do Rio de Janeiro. Accessed January 19, 2021.
http://www.bdtd.uerj.br/tde_busca/arquivo.php?codArquivo=2167 ;.
MLA Handbook (7th Edition):
Moss, Monique Bandeira. “Mecanismos moleculares e celulares envolvidos na modulação da via L-arginina-óxido nítrico em hipertensão e insuficiência renal crônica.” 2010. Web. 19 Jan 2021.
Vancouver:
Moss MB. Mecanismos moleculares e celulares envolvidos na modulação da via L-arginina-óxido nítrico em hipertensão e insuficiência renal crônica. [Internet] [Doctoral dissertation]. Universidade do Estado do Rio de Janeiro; 2010. [cited 2021 Jan 19].
Available from: http://www.bdtd.uerj.br/tde_busca/arquivo.php?codArquivo=2167 ;.
Council of Science Editors:
Moss MB. Mecanismos moleculares e celulares envolvidos na modulação da via L-arginina-óxido nítrico em hipertensão e insuficiência renal crônica. [Doctoral Dissertation]. Universidade do Estado do Rio de Janeiro; 2010. Available from: http://www.bdtd.uerj.br/tde_busca/arquivo.php?codArquivo=2167 ;
25.
Prati, Clément.
Rôle de l'arginase dans l'atteinte vasculaire associée à l'arthrite chez le rat : Role of arginase in vascular disease associated with arthritis in rats.
Degree: Docteur es, Sciences de la vie et de la santé, 2012, Besançon
URL: http://www.theses.fr/2012BESA3017
► Les patients atteints de polyarthrite rhumatoide (PR) ont une diminution de l'espérance de vie de 10 à 15 ans. Cette augmentation de la mortalité semble…
(more)
▼ Les patients atteints de polyarthrite rhumatoide (PR) ont une diminution de l'espérance de vie de 10 à 15 ans. Cette augmentation de la mortalité semble liée à un processus athéromateux accéléré. La dysfonction endothéliale (DE) joue un rôle clé dans ce processus. L'arginase est une enzyme qui régule le niveau de NO par compétition avec la NO Synthase (NOS) pour leur substrat commun, la L-arginine.Nous avons montré que la vasodilatation Acétylcholine (ACh) dépendante était altérée dans le modèle d'Arthrite Induite à Adjuvant (AIA) chez le rat Lewis, témoignant d'une DE. L'incubation des anneaux aortiques avec la nor-NOHA un inhibiteur sélectif d'arginase a amélioré la réponse vasculaire à l'ACh chez les rats AIA. L'activité et l'expression vasculaire de l'arginase se sont révélées augmentées chez les rats AIA et corrélées positivement à la sévérité de l'arthrite.Nous avons caractérisé les mécanismes impliqués dans la DE du rat AIA. Nos résultats ont montré que la DE mettait enjeu une diminution de l'activité de la NO synthase, un déficit en EDHF, une suractivité de la COX-2, ainsi qu'une production excessive des anions superoxydes. Le traitement curatif des rats AIA par la nor-NOHA pendant 3 semaines, a permis de restaurer la fonction endothéliale. L'inhibition de l'arginase n'a pas modifié l'atteinte articulaire des rats AIA.Nos travaux ont permis de mieux comprendre la physiopathologie de la DE associée à la PR et ont déterminé pour la première fois le rôle délétère de l'arginase dans cette anomalie. Ils ouvrent des perspectives quant à l'utilisation des inhibiteurs d'arginase comme futurs traitements pharmacologiques de l'atteinte vasculaire du patient PR
Patients with RA are characterized by a decrease in life expectancy of 10 to 15 years. This increase in mortality seems to be related to an accelerated atheroma process. Endothelial dysfunction (ED) has a key role in these processes. The arginase is an enzyme which regulates the level of NO by competing with the NO synthase (NOS) to their common substrate, L-arginine.We showed that acetylcholine (ACh) dependent vasodilation was altered in the model of Adjuvant Induced Arthritis (AIA) in Lewis rats, indicating a endothelial dysfunction. The incubation of aortic rings with nor-NOHA has improved the vascular response to ACh in AIA rats. The activity and expression of vascular arginase are increased in AIA rats and positively correlated with the severity of arthritis.We characterized the mechanisms involved in DE in AIA rats. Our results showed that ED involved a decrease of activity of NO synthase, a decrease of EDHF, overactiviry of COX-2, thromboxane synthase and prostacyclin synthase, as well as excessive superoxide anions. The cure of AIA rats by a selective inhibitor of arginase, nor-NOHA for 3 weeks, has restored endothelial function. In contrast, inhibition of arginase activity did not change the weight, the diameter of ankles, radiological or histological articular damage in AIA rats.Our work has led to a better understanding of pathophysiology of ED…
Advisors/Committee Members: Demougeot, Céline (thesis director), Wendling, Daniel (thesis director).
Subjects/Keywords: Polyarthrite rhumatoïde; Arthrite induite à adjuvant; Rat; Arginase; Dysfonction endothéliale; Nor NOHA; Rheumatoid arthritis; Adjuvant-induced arthritis; Rat; Arginase; Endothelial dysfunction; Nor NOHA; 616.7; WE 346
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APA ·
Chicago ·
MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Prati, C. (2012). Rôle de l'arginase dans l'atteinte vasculaire associée à l'arthrite chez le rat : Role of arginase in vascular disease associated with arthritis in rats. (Doctoral Dissertation). Besançon. Retrieved from http://www.theses.fr/2012BESA3017
Chicago Manual of Style (16th Edition):
Prati, Clément. “Rôle de l'arginase dans l'atteinte vasculaire associée à l'arthrite chez le rat : Role of arginase in vascular disease associated with arthritis in rats.” 2012. Doctoral Dissertation, Besançon. Accessed January 19, 2021.
http://www.theses.fr/2012BESA3017.
MLA Handbook (7th Edition):
Prati, Clément. “Rôle de l'arginase dans l'atteinte vasculaire associée à l'arthrite chez le rat : Role of arginase in vascular disease associated with arthritis in rats.” 2012. Web. 19 Jan 2021.
Vancouver:
Prati C. Rôle de l'arginase dans l'atteinte vasculaire associée à l'arthrite chez le rat : Role of arginase in vascular disease associated with arthritis in rats. [Internet] [Doctoral dissertation]. Besançon; 2012. [cited 2021 Jan 19].
Available from: http://www.theses.fr/2012BESA3017.
Council of Science Editors:
Prati C. Rôle de l'arginase dans l'atteinte vasculaire associée à l'arthrite chez le rat : Role of arginase in vascular disease associated with arthritis in rats. [Doctoral Dissertation]. Besançon; 2012. Available from: http://www.theses.fr/2012BESA3017
Univerzitet u Beogradu
26.
Stojiljković, Mojca D., 1967-.
Efekat eksperimentalnog dijabetesa tipa 1 i supstitucione
terapije na molekule regulisane insulinom u srcu pacova.
Degree: Biološki fakultet, 2016, Univerzitet u Beogradu
URL: https://fedorabg.bg.ac.rs/fedora/get/o:12801/bdef:Content/get
► Biologija - Molekularna endokrinologija / Biology - Molecular endocrinology
Insulinska deficijencija i hiperglikemija, koje predstavljaju osnovne karakteristike dijabetesa tipa 1, povezane su sa brojnim endokrinim…
(more)
▼ Biologija - Molekularna endokrinologija / Biology -
Molecular endocrinology
Insulinska deficijencija i hiperglikemija, koje
predstavljaju osnovne karakteristike dijabetesa tipa 1, povezane su
sa brojnim endokrinim i metaboličkim promenama, a jedna od
najčešćih komplikacija je povećana učestalost nastanka
kardiovaskularnih oboljenja. Zbog toga je rana i adekvatna primena
terapije insulinom neophodna, kako za kontrolu bolesti, tako i za
prevenciju potencijalnih komplikacija. Polazna hipoteza
istraţivanja čiji su rezultati obuhvaćeni ovom doktorskom
disertacijom je bila da u stanju hipoinsulinemije i hiperglikemije
moţe doći do promena u signalnom putu insulina u srcu, što moţe da
se odrazi na produkciju azot-monoksida i iskorišćavanje energetskih
supstrata, a da bi supstitucija insulinom trebala da koriguje
nastale promene. Za uspostavljanje eksperimentalnog modela
dijabetesa tipa 1, muţjaci pacova soja Wistar tretirani su
streptozotocinom (60 mg/kg). Poznato je da se akutni dijabetes
javlja između osmog dana i tri nedelje, a hronični 3 nedelje nakon
tretmana streptozotocinom. Ţivotinje sa dijabetesom su ili bile
netretirane 2 nedelje ili podvrgnute subhroničnom tretmanu
insulinom (3 IU) u trajanju od 7 dana. Za analizu su selektirani
signalni molekuli Akt i ERK koji predstavljaju medijatore gotovo
svih efekata insulina u srcu. Osim toga analizirani su i efektorni
molekuli regulisani preko aktivacije signalnih puteva Akt i ERK,
kao što su eNOS i iNOS, uključeni u sintezu azot-monoksida, kao i
transporteri glukoze GLUT1 i GLUT4 i transporter masnih kiselina
CD36. Ispitivana je ekspresija ovih molekula na nivou gena i
proteina i/ili fosforilacija, kao i unutarćelijska lokalizacija
transportera za glukozu i masne kiseline u srcu. Poznato je da je
kompeticija između arginaze i NOS za zajednički supstrat L-arginin,
ograničavajući faktor za proizvodnju
azot-monoksida...
Advisors/Committee Members: Žakula, Zorica, 1955-.
Subjects/Keywords: Type 1 diabetes; insulin; heart; Akt; ERK1/2; arginase;
NOS; GLUT4; GLUT1; CD36
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APA ·
Chicago ·
MLA ·
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CSE |
Export
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APA (6th Edition):
Stojiljković, Mojca D., 1. (2016). Efekat eksperimentalnog dijabetesa tipa 1 i supstitucione
terapije na molekule regulisane insulinom u srcu pacova. (Thesis). Univerzitet u Beogradu. Retrieved from https://fedorabg.bg.ac.rs/fedora/get/o:12801/bdef:Content/get
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Stojiljković, Mojca D., 1967-. “Efekat eksperimentalnog dijabetesa tipa 1 i supstitucione
terapije na molekule regulisane insulinom u srcu pacova.” 2016. Thesis, Univerzitet u Beogradu. Accessed January 19, 2021.
https://fedorabg.bg.ac.rs/fedora/get/o:12801/bdef:Content/get.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Stojiljković, Mojca D., 1967-. “Efekat eksperimentalnog dijabetesa tipa 1 i supstitucione
terapije na molekule regulisane insulinom u srcu pacova.” 2016. Web. 19 Jan 2021.
Vancouver:
Stojiljković, Mojca D. 1. Efekat eksperimentalnog dijabetesa tipa 1 i supstitucione
terapije na molekule regulisane insulinom u srcu pacova. [Internet] [Thesis]. Univerzitet u Beogradu; 2016. [cited 2021 Jan 19].
Available from: https://fedorabg.bg.ac.rs/fedora/get/o:12801/bdef:Content/get.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Stojiljković, Mojca D. 1. Efekat eksperimentalnog dijabetesa tipa 1 i supstitucione
terapije na molekule regulisane insulinom u srcu pacova. [Thesis]. Univerzitet u Beogradu; 2016. Available from: https://fedorabg.bg.ac.rs/fedora/get/o:12801/bdef:Content/get
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
27.
Mabley, Jon Gunnarsson.
Cytokine and growth factor modulation of nitric oxide production and effects in rat islets of Langerhans and insulin-secreting cell lines.
Degree: PhD, 1996, University of Sussex
URL: https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.360535
Subjects/Keywords: 572; Interleukin-1; Arginase; Free radicals
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APA ·
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APA (6th Edition):
Mabley, J. G. (1996). Cytokine and growth factor modulation of nitric oxide production and effects in rat islets of Langerhans and insulin-secreting cell lines. (Doctoral Dissertation). University of Sussex. Retrieved from https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.360535
Chicago Manual of Style (16th Edition):
Mabley, Jon Gunnarsson. “Cytokine and growth factor modulation of nitric oxide production and effects in rat islets of Langerhans and insulin-secreting cell lines.” 1996. Doctoral Dissertation, University of Sussex. Accessed January 19, 2021.
https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.360535.
MLA Handbook (7th Edition):
Mabley, Jon Gunnarsson. “Cytokine and growth factor modulation of nitric oxide production and effects in rat islets of Langerhans and insulin-secreting cell lines.” 1996. Web. 19 Jan 2021.
Vancouver:
Mabley JG. Cytokine and growth factor modulation of nitric oxide production and effects in rat islets of Langerhans and insulin-secreting cell lines. [Internet] [Doctoral dissertation]. University of Sussex; 1996. [cited 2021 Jan 19].
Available from: https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.360535.
Council of Science Editors:
Mabley JG. Cytokine and growth factor modulation of nitric oxide production and effects in rat islets of Langerhans and insulin-secreting cell lines. [Doctoral Dissertation]. University of Sussex; 1996. Available from: https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.360535
University of Western Ontario
28.
Poirier, Jacob W.
The Role of GABA Signalling in Lung Macrophage Immune Response.
Degree: 2016, University of Western Ontario
URL: https://ir.lib.uwo.ca/etd/4154
► Lung macrophages (LMϕs) play a key role in pulmonary innate immunity. They polarize into different phenotypes adapting to the needs of the immediate pulmonary environment.…
(more)
▼ Lung macrophages (LMϕs) play a key role in pulmonary innate immunity. They polarize into different phenotypes adapting to the needs of the immediate pulmonary environment. Studies in our laboratory suggest that murine LMϕs are endowed with an autocrine gamma-aminobutyric acid (GABA) signaling system. My honors thesis study found that antagonizing the autocrine GABA signaling in alveolar macrophages (AMϕs) increased secretion of the M1 cytokine tumor necrosis factor-alpha (TNF-α), suggesting a role for GABA signaling in immune response. This project explored whether GABA signaling plays a role in LMϕ polarization. Results from this study confirmed that bacterial toxin lipopolysaccharide (LPS) and the Th1 cytokine interferon gamma (IFNγ) shifted LMϕs to the pro-inflammatory M1 phenotype, marked by increased expression of inducible nitric oxide synthase (iNOS). On the other hand, the Th2 cytokines interleukin (IL)-4 and IL-13 shifted LMϕs toward the M2 phenotype marked by increased arginase-1. Importantly, in both RAW 264.7 cell line and primary LMϕs, LPS and IFNγ treatment increased iNOS expression while decreasing glutamic acid decarboxylase (GAD) and A-type GABA receptor α2-subunit (α2-GABAAR). Conversely, treatment with IL4/13 induced an upregulation of arginase-1, GAD, and α2-GABAAR. Moreover, treatment of primary LMϕs with IL4/13 and GABAAR antagonist picrotoxin decreased arginase-1 and GAD expression, and increased iNOS levels. These results suggest the autocrine GABA signaling system in LMϕs dynamically changes with their phenotypic polarization. This signaling system functions to limit the M1 response, but facilitate M2 reaction, and thus a change in the GABA signaling may alter the inflammatory responses of these cells.
Subjects/Keywords: Macrophage polarization; Alveolar macrophage; GABA signalling; Pulmonary inflammation; iNOS; Arginase-1; Cellular and Molecular Physiology
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APA ·
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Export
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Manager
APA (6th Edition):
Poirier, J. W. (2016). The Role of GABA Signalling in Lung Macrophage Immune Response. (Thesis). University of Western Ontario. Retrieved from https://ir.lib.uwo.ca/etd/4154
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Poirier, Jacob W. “The Role of GABA Signalling in Lung Macrophage Immune Response.” 2016. Thesis, University of Western Ontario. Accessed January 19, 2021.
https://ir.lib.uwo.ca/etd/4154.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Poirier, Jacob W. “The Role of GABA Signalling in Lung Macrophage Immune Response.” 2016. Web. 19 Jan 2021.
Vancouver:
Poirier JW. The Role of GABA Signalling in Lung Macrophage Immune Response. [Internet] [Thesis]. University of Western Ontario; 2016. [cited 2021 Jan 19].
Available from: https://ir.lib.uwo.ca/etd/4154.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Poirier JW. The Role of GABA Signalling in Lung Macrophage Immune Response. [Thesis]. University of Western Ontario; 2016. Available from: https://ir.lib.uwo.ca/etd/4154
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
29.
Baldo, Salvatore.
Development and characterization of nanocarbon-based devices for sensing applications.
Degree: 2017, Università degli Studi di Catania
URL: http://hdl.handle.net/10761/3750
► The subject of this PhD thesis has been the development and characterization of nanocarbon-based sensors, with particular attention to the use of easy and cheap…
(more)
▼ The subject of this PhD thesis has been the development and characterization of nanocarbon-based sensors, with particular attention to the use of easy and cheap methodologies and processes that are required for future large scale production (dielectrophoresis, lift-off, etc..). In the first part of the thesis the development of Carbon nanotubes (CNTs)-based devices for biosensing applications is reported and the tests on protein detection are shown. In the second part the development of Graphene oxide (GO)-based devices is shown and the electrical behavior of the devices is investigated in different ambient conditions and for different oxygen functionalization (GO and reduced GO).
The thesis work is divided as follows:
In the first chapter the Carbon-like materials basics are recalled. In particular the types, the properties, and the main synthesis methods are described;
The second chapter introduces the FET/Resistor devices and then describes the most common CNTs/GO structures in literature;
In the third chapter, the methods for devices preparation are described with a focus on the dielectrophoretic process, used for the deposition of the carbon-based sensitive layers. Therefore, the characterization of the devices and the sensing properties achieved as immunosensors, or humidity/temperature and volatile organic compounds (VOC) sensors are shown and discussed;
Finally, in the conclusions the main results, work in progress, developments and future applications are discussed.
Subjects/Keywords: Area 02 - Scienze fisiche; cnt, graphene, graphene oxide, sensor, arginase, dielectrophoresis, cntfet, biosensor, nanomaterial, carbon
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APA ·
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APA (6th Edition):
Baldo, S. (2017). Development and characterization of nanocarbon-based devices for sensing applications. (Thesis). Università degli Studi di Catania. Retrieved from http://hdl.handle.net/10761/3750
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Baldo, Salvatore. “Development and characterization of nanocarbon-based devices for sensing applications.” 2017. Thesis, Università degli Studi di Catania. Accessed January 19, 2021.
http://hdl.handle.net/10761/3750.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Baldo, Salvatore. “Development and characterization of nanocarbon-based devices for sensing applications.” 2017. Web. 19 Jan 2021.
Vancouver:
Baldo S. Development and characterization of nanocarbon-based devices for sensing applications. [Internet] [Thesis]. Università degli Studi di Catania; 2017. [cited 2021 Jan 19].
Available from: http://hdl.handle.net/10761/3750.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Baldo S. Development and characterization of nanocarbon-based devices for sensing applications. [Thesis]. Università degli Studi di Catania; 2017. Available from: http://hdl.handle.net/10761/3750
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Kansas State University
30.
Malalasekera, Aruni Peiris.
Development
of novel therapeutic and diagnostic approaches utilizing tools from
the physical sciences.
Degree: PhD, Department of
Chemistry, 2016, Kansas State University
URL: http://hdl.handle.net/2097/35779
► Numerous Proteases are implicated in cancer initiation, survival, and progression. Therefore, it is important to diagnose the levels of protease expression by tumors and surrounding…
(more)
▼ Numerous Proteases are implicated in cancer
initiation, survival, and progression. Therefore, it is important
to diagnose the levels of protease expression by tumors and
surrounding tissues, which are reflected in blood and tissue
samples. Nanoplatforms for Cathepsin(CTS) B and L, matrix
metalloproteinases(MMP) 1, 2, 3, 7, 9, 13 and urokinase plasminogen
activator(uPA) detection have been synthesized. Nanoplatforms
feature a central dopamine-coated core/shell Fe/Fe₃O₄ nanoparticle.
Cyanine 5.5 is permanently tethered to the dopamine ligands via
amide bonds. Tetrakis(4-carboxy-phenyl)porphyrin (TCPP) is
co-tethered to Fe/Fe₃O₄/dopamine by means of protease consensus
sequences. In the presence of a relevant protease sequence, it is
cleaved, releasing TCPP from the nanoplatform. In contrast, Cy 5.5
will remain permanently tethered to the nanoparticle. Therefore, an
extensive increase of emission intensity of the fluorescence signal
from TCPP is observed. This permits the detection of the activity
of proteases at femtomolar levels in biospecimens by fluorescence
spectroscopy. 46 breast cancer and 20 healthy human blood serum
samples were analyzed. Based on the expression pattern of analyzed
enzymes, human breast cancer can be detected at stage I. By
monitoring CTS B and L stage 0 detection may be achieved. This
study demonstrates the feasibility of minimally invasive successful
early cancer diagnosis.
Immunosuppression is one of the hallmarks
of aggressive cancers.
Arginase is overexpressed in cancer
patients, resulting in systemic immunosuppression. Two
nanoplatforms for
arginase detection have been synthesized. Both
feature a central dopamine-coated core/shell Fe/Fe₃O₄ nanoparticle
to which cyanine 7.0 or cyanine 7.5 is tethered via amide bonds. In
both nanoplatforms, cyanine 5.5 is linked to the N-terminal of the
peptide sequence GRRRRRRRG. Arginine (R) reacts to ornithine (O) in
the presence of
arginase. According to our results
obtained from
fluorescence spectroscopy, the oligopeptides GRRRRRRRG and
GOOOOOOOG differ in their chain dynamics. In the presence of
arginase, and dependent on
arginase activity, fluorescence increase
of both nanoplatforms is observed, which is an indication that
proton-transfer quenching decreases when arginine gets converted to
ornithine. The novel assays permit the detection of active
arginase
within an hour. Additionally, Förster Resonance Energy Transfer
(FRET) is observed in nanoplatforms featuring cy 5.5/7.0 pairs,
resulting in picomolar detection limits. This is the first example
of a “post-translational” enzyme sensor, in which the tether is
subjected to chemical transformations of the aminoacid side chains
and not cleaved by an enzyme, resulting in the modified mobility of
the tether. The nanoplatforms do not show a fluorescence increase
when incubated with NO-reductase, an enzyme indicative of
immunoactivation, which also uses
arginase as substrate.
Copper
dependent inhibitory activity of 10000 compound library has been
studied against of Staphylococcus aureus. 53 copper-…
Advisors/Committee Members: Stefan Bossmann.
Subjects/Keywords: Breast
cancer diagnostics;
Biophotonics; High
throughput diagnostics;
Copper-dependent drug activation;
Nanophotonics; Arginase
detection
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APA (6th Edition):
Malalasekera, A. P. (2016). Development
of novel therapeutic and diagnostic approaches utilizing tools from
the physical sciences. (Doctoral Dissertation). Kansas State University. Retrieved from http://hdl.handle.net/2097/35779
Chicago Manual of Style (16th Edition):
Malalasekera, Aruni Peiris. “Development
of novel therapeutic and diagnostic approaches utilizing tools from
the physical sciences.” 2016. Doctoral Dissertation, Kansas State University. Accessed January 19, 2021.
http://hdl.handle.net/2097/35779.
MLA Handbook (7th Edition):
Malalasekera, Aruni Peiris. “Development
of novel therapeutic and diagnostic approaches utilizing tools from
the physical sciences.” 2016. Web. 19 Jan 2021.
Vancouver:
Malalasekera AP. Development
of novel therapeutic and diagnostic approaches utilizing tools from
the physical sciences. [Internet] [Doctoral dissertation]. Kansas State University; 2016. [cited 2021 Jan 19].
Available from: http://hdl.handle.net/2097/35779.
Council of Science Editors:
Malalasekera AP. Development
of novel therapeutic and diagnostic approaches utilizing tools from
the physical sciences. [Doctoral Dissertation]. Kansas State University; 2016. Available from: http://hdl.handle.net/2097/35779
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