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You searched for subject:(anti carcinogenic). Showing records 1 – 3 of 3 total matches.

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The Ohio State University

1. Arango Tamayo, Daniel Alejandro. The Immune-modulatory and Anti-carcinogenic Mechanisms of the Flavonoid Apigenin.

Degree: PhD, Molecular, Cellular and Developmental Biology, 2015, The Ohio State University

Dietary phytochemicals provide health benefits against several cancers and inflammatory diseases. Flavonoids are amongst the most abundant dietary phytochemicals emerging as key anti-carcinogenic and anti-inflammatory molecules. Yet, the mechanisms underlying their anti-cancer and anti-inflammatory activities are poorly defined. The goal of this project was to study the immune-modulatory and anti-carcinogenic mechanisms of the flavonoid apigenin. I investigated the modes of action of apigenin using different model systems including a monocytic leukemia cell line, breast cancer cell lines, macrophages and mouse models of inflammation and breast cancer development. In monocytic leukemia, I found that apigenin induces DNA strand breaks leading to the activation of a DNA damage response pathway that results in cell cycle arrest and induction of apoptosis. Using mouse models of inflammation, I showed that apigenin reduces lipopolysaccharide (LPS)-induced lethality by inhibiting the activity of the transcription factor NF-kB and the expression of the pro-inflammatory molecules miR-155 and TNFalpha. I established, using a pre-clinical mouse model of breast cancer development, that the immune-modulatory and anti-carcinogenic activities of apigenin work in concert to delay breast tumor progression and metastasis by dually acting on malignant and immune cells. My results show that apigenin induces apoptosis and inhibits proliferation in breast tumors as well as halts macrophages infiltration in the tumor microenvironment by reducing the expression of NF-kB-dependent chemokines and promoting apoptosis in blood monocytes, the macrophage progenitors. Moreover, I implemented the use of a newly formulated celery-based apigenin-rich diet in mouse models of inflammation and breast cancer demonstrating that this diet, as well as apigenin, have anti-inflammatory and anti-carcinogenic activities by immune-modulating monocytes and macrophages and inducing apoptosis in cancer cells. To investigate the molecular mechanisms underlying the biological effects of apigenin, we developed of a new genome-wide approach to identify direct targets of this flavonoid. From these studies, I identified 160 candidate targets of apigenin that revealed unexpected mechanisms on how this dietary phytochemical modulates cellular functions such as apoptosis, immune and DNA damage response signaling pathways. In addition, I observed that apigenin interacts with RNA binding proteins including the heterogeneous nuclear RiboNucleoProtein A2 (hnRNPA2) and affects splicing genome-wide, providing a novel mechanism on how this flavone regulates apoptotic cell fate through modulation of mRNA processing. Altogether, this investigation offers a fresh view on how flavonoids influence human health, by impacting multiple cellular targets with moderate affinity. Thus, in contrast to pharmaceutical drugs selected to have high affinity and specificity for main hubs of biological pathways, the effect of flavonoids would be distributed across the entire cellular network with… Advisors/Committee Members: Doseff, Andrea (Advisor).

Subjects/Keywords: Molecular Biology; Apigenin; Flavonoids; Immune-modulatory; Anti-carcinogenic; Diet

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APA (6th Edition):

Arango Tamayo, D. A. (2015). The Immune-modulatory and Anti-carcinogenic Mechanisms of the Flavonoid Apigenin. (Doctoral Dissertation). The Ohio State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=osu1437511798

Chicago Manual of Style (16th Edition):

Arango Tamayo, Daniel Alejandro. “The Immune-modulatory and Anti-carcinogenic Mechanisms of the Flavonoid Apigenin.” 2015. Doctoral Dissertation, The Ohio State University. Accessed December 05, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=osu1437511798.

MLA Handbook (7th Edition):

Arango Tamayo, Daniel Alejandro. “The Immune-modulatory and Anti-carcinogenic Mechanisms of the Flavonoid Apigenin.” 2015. Web. 05 Dec 2020.

Vancouver:

Arango Tamayo DA. The Immune-modulatory and Anti-carcinogenic Mechanisms of the Flavonoid Apigenin. [Internet] [Doctoral dissertation]. The Ohio State University; 2015. [cited 2020 Dec 05]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1437511798.

Council of Science Editors:

Arango Tamayo DA. The Immune-modulatory and Anti-carcinogenic Mechanisms of the Flavonoid Apigenin. [Doctoral Dissertation]. The Ohio State University; 2015. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1437511798

2. Lakshmi B. Antimutagenic and anti carcinogenic activities of selected mushrooms from Kerala;.

Degree: Microbiology, 2014, University of Calicut

newline

References p.139-159

Advisors/Committee Members: K.K. Janardanan.

Subjects/Keywords: Anti carcinogenic activity; Antimutagenic activity; Microbiology; Mushroom

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

B, L. (2014). Antimutagenic and anti carcinogenic activities of selected mushrooms from Kerala;. (Thesis). University of Calicut. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/31385

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

B, Lakshmi. “Antimutagenic and anti carcinogenic activities of selected mushrooms from Kerala;.” 2014. Thesis, University of Calicut. Accessed December 05, 2020. http://shodhganga.inflibnet.ac.in/handle/10603/31385.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

B, Lakshmi. “Antimutagenic and anti carcinogenic activities of selected mushrooms from Kerala;.” 2014. Web. 05 Dec 2020.

Vancouver:

B L. Antimutagenic and anti carcinogenic activities of selected mushrooms from Kerala;. [Internet] [Thesis]. University of Calicut; 2014. [cited 2020 Dec 05]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/31385.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

B L. Antimutagenic and anti carcinogenic activities of selected mushrooms from Kerala;. [Thesis]. University of Calicut; 2014. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/31385

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

3. Dansby, Montreka. Evaluation of the Antioxidant and Biological Properties of Muscadine Grape Seed Extracts.

Degree: PhD, Food Science, 2007, North Carolina State University

The objectives of this research were i) to optimize extraction of phenolic compounds from muscadine grape seeds using food grade solvents, ii) to characterize and quantify the phenolic compounds present in Carlos muscadine grape seed extracts, iii) to assess the oxidative stability of the grape seed extract, iv) to evaluate the antimicrobial activity of the optimal muscadine grape seed extract, and v) to evaluate the effect of muscadine grape seed extract on MCF-10A breast cells. Carlos muscadine grape seeds were manually removed from the skin and pulp, then freeze-dried or oven-dried. Phenolic compounds were extracted from muscadine grape seeds using a Dionex Accelerated Solvent Extractor 200 following a two factor composite design. The two factors were solvent and temperature. Ethanol in combination with water and ethyl acetate in combination with water were evaluated in this research. Solvent percentages ranged from 35% to 95% and temperature ranged from 75C to 150C. Optimal extraction conditions were determined using the Folin-Ciocalteu and oxygen radical absorbance capacity assays. Identification and quantification of the phenolic compounds were determined by high performance liquid chromatography with mass spectroscopy. The optimal extraction conditions for the highest recovery of phenolic compounds consisted of the freeze-dried seeds extracted with 65% ethanol and 35% water at 150°C, with total phenolic and oxygen radical absorbance capacity values of 5.87 mg GAE⁄g and 60.32 M-TE⁄g of seeds, respectively. The four major phenolic compounds quantified in the muscadine grape seed extract were epicatechin, catechin, gallic acid and ellagic acid. Oxidative stability index of the muscadine grape seed extract resulted in an induction period of 4.6 hours. In the antimicrobial study, both muscadine grape seed extract and ellagic acid inhibited the growth of E. coli 0157:H7, Listeria monocytogenes, and Salmonella typhimurium at 104 cfu⁄ml. These research findings show that muscadine grape seed extracts contain phytochemicals with antioxidant and antimicrobial properties that can be used in applications including food ingredients, food supplements and other nutraceutical applications. Advisors/Committee Members: Dr. Leon Boyd, Committee Chair (advisor).

Subjects/Keywords: antioxidants; anti-microbial; anti-carcinogenic; grape seed; polyphenols; muscadines

…grapes and grape products) exhibited anti-carcinogenic properties by inducing cell cycle… …such as antiinflammatory, anticarcinogenic and anti-microbial properties. There is… …concluded that lipid profiles responded significantly more anti-atherogenic on red wine compared… …functions in modulating physiological and pathological reactions of the body, such as anti-cancer… …anti-mutagenesis, and cardioprotection (Slater et al., 2003). Anticarcinogenic… 

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Dansby, M. (2007). Evaluation of the Antioxidant and Biological Properties of Muscadine Grape Seed Extracts. (Doctoral Dissertation). North Carolina State University. Retrieved from http://www.lib.ncsu.edu/resolver/1840.16/5328

Chicago Manual of Style (16th Edition):

Dansby, Montreka. “Evaluation of the Antioxidant and Biological Properties of Muscadine Grape Seed Extracts.” 2007. Doctoral Dissertation, North Carolina State University. Accessed December 05, 2020. http://www.lib.ncsu.edu/resolver/1840.16/5328.

MLA Handbook (7th Edition):

Dansby, Montreka. “Evaluation of the Antioxidant and Biological Properties of Muscadine Grape Seed Extracts.” 2007. Web. 05 Dec 2020.

Vancouver:

Dansby M. Evaluation of the Antioxidant and Biological Properties of Muscadine Grape Seed Extracts. [Internet] [Doctoral dissertation]. North Carolina State University; 2007. [cited 2020 Dec 05]. Available from: http://www.lib.ncsu.edu/resolver/1840.16/5328.

Council of Science Editors:

Dansby M. Evaluation of the Antioxidant and Biological Properties of Muscadine Grape Seed Extracts. [Doctoral Dissertation]. North Carolina State University; 2007. Available from: http://www.lib.ncsu.edu/resolver/1840.16/5328

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