subject:(actomyosin)

Ruhr Universität Bochum

1. Qu, Zheng. Purification of chemically cross-linked actin oligomers and their interaction with actin binding proteins and myosin heads.

Degree: 2014, Ruhr Universität Bochum

URL: http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-42382

► Das chemische Reagenz, PBM (Phenylenebismaleimide), kann Lysine-191 und Cystein-374 zwischen zwei nebeneinanderliegende Aktin-Untereinheiten in der genetischen Helix der Aktin Filamente quervernetzen. Die Aktin Oligonmere wurden… (more)

Subjects/Keywords: Actin; Myosin; Actomyosin; Actin-bindende Proteine; Gelsolin

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APA (6^th Edition):

Qu, Z. (2014). Purification of chemically cross-linked actin oligomers and their interaction with actin binding proteins and myosin heads. (Thesis). Ruhr Universität Bochum. Retrieved from http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-42382

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Qu, Zheng. “Purification of chemically cross-linked actin oligomers and their interaction with actin binding proteins and myosin heads.” 2014. Thesis, Ruhr Universität Bochum. Accessed March 01, 2021. http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-42382.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Qu, Zheng. “Purification of chemically cross-linked actin oligomers and their interaction with actin binding proteins and myosin heads.” 2014. Web. 01 Mar 2021.

Vancouver:

Qu Z. Purification of chemically cross-linked actin oligomers and their interaction with actin binding proteins and myosin heads. [Internet] [Thesis]. Ruhr Universität Bochum; 2014. [cited 2021 Mar 01]. Available from: http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-42382.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Qu Z. Purification of chemically cross-linked actin oligomers and their interaction with actin binding proteins and myosin heads. [Thesis]. Ruhr Universität Bochum; 2014. Available from: http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-42382

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Guelph

2. Mishra, Vadika. Characterization of short ADP ribosylated oligomers developed by modification in F-actin structure.

Degree: MS, Department of Molecular and Cellular Biology, 2015, University of Guelph

URL: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/8714

► Researchers have been trying to determine the mechanism of the crossbridge cycle, containing actomyosin complex, formed by the interaction of F-actin and myosin. But, because… (more)

Subjects/Keywords: actin; myosin; interaction; actomyosin; F-actin

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APA (6^th Edition):

Mishra, V. (2015). Characterization of short ADP ribosylated oligomers developed by modification in F-actin structure. (Masters Thesis). University of Guelph. Retrieved from https://atrium.lib.uoguelph.ca/xmlui/handle/10214/8714

Chicago Manual of Style (16^th Edition):

Mishra, Vadika. “Characterization of short ADP ribosylated oligomers developed by modification in F-actin structure.” 2015. Masters Thesis, University of Guelph. Accessed March 01, 2021. https://atrium.lib.uoguelph.ca/xmlui/handle/10214/8714.

MLA Handbook (7^th Edition):

Mishra, Vadika. “Characterization of short ADP ribosylated oligomers developed by modification in F-actin structure.” 2015. Web. 01 Mar 2021.

Vancouver:

Mishra V. Characterization of short ADP ribosylated oligomers developed by modification in F-actin structure. [Internet] [Masters thesis]. University of Guelph; 2015. [cited 2021 Mar 01]. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/8714.

Council of Science Editors:

Mishra V. Characterization of short ADP ribosylated oligomers developed by modification in F-actin structure. [Masters Thesis]. University of Guelph; 2015. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/8714

3. McFadden, Maureen. Rôle du cytosquelette actomyosine dans les effets synaptiques du récepteur cannabinoïde de type-1 (CB1R) : Role of the actomyosin cytoskeleton on the synaptic effects of the type-1 cannabinoid receptor (CB1R).

Degree: Docteur es, Neurosciences, 2018, Paris Sciences et Lettres (ComUE)

URL: http://www.theses.fr/2018PSLET006

►

Des résultats récents de l'équipe d’accueil suggèrent que l'activation du récepteur cannabinoid de type 1 (CB1R) induit une restructuration importante des neurones aux travers du… (more)

Subjects/Keywords: Actomyosine; Synapse; Cb1r; Actomyosin; Cb1r; Synapse; 570

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APA (6^th Edition):

McFadden, M. (2018). Rôle du cytosquelette actomyosine dans les effets synaptiques du récepteur cannabinoïde de type-1 (CB1R) : Role of the actomyosin cytoskeleton on the synaptic effects of the type-1 cannabinoid receptor (CB1R). (Doctoral Dissertation). Paris Sciences et Lettres (ComUE). Retrieved from http://www.theses.fr/2018PSLET006

Chicago Manual of Style (16^th Edition):

McFadden, Maureen. “Rôle du cytosquelette actomyosine dans les effets synaptiques du récepteur cannabinoïde de type-1 (CB1R) : Role of the actomyosin cytoskeleton on the synaptic effects of the type-1 cannabinoid receptor (CB1R).” 2018. Doctoral Dissertation, Paris Sciences et Lettres (ComUE). Accessed March 01, 2021. http://www.theses.fr/2018PSLET006.

MLA Handbook (7^th Edition):

McFadden, Maureen. “Rôle du cytosquelette actomyosine dans les effets synaptiques du récepteur cannabinoïde de type-1 (CB1R) : Role of the actomyosin cytoskeleton on the synaptic effects of the type-1 cannabinoid receptor (CB1R).” 2018. Web. 01 Mar 2021.

Vancouver:

McFadden M. Rôle du cytosquelette actomyosine dans les effets synaptiques du récepteur cannabinoïde de type-1 (CB1R) : Role of the actomyosin cytoskeleton on the synaptic effects of the type-1 cannabinoid receptor (CB1R). [Internet] [Doctoral dissertation]. Paris Sciences et Lettres (ComUE); 2018. [cited 2021 Mar 01]. Available from: http://www.theses.fr/2018PSLET006.

Council of Science Editors:

McFadden M. Rôle du cytosquelette actomyosine dans les effets synaptiques du récepteur cannabinoïde de type-1 (CB1R) : Role of the actomyosin cytoskeleton on the synaptic effects of the type-1 cannabinoid receptor (CB1R). [Doctoral Dissertation]. Paris Sciences et Lettres (ComUE); 2018. Available from: http://www.theses.fr/2018PSLET006

University of North Texas

4. Gundapaneni, Deepika. Studies on actomyosin crossbridge flexibility using a new single molecule assay.

Degree: 2004, University of North Texas

URL: https://digital.library.unt.edu/ark:/67531/metadc4514/

► Several key flexure sites exist in the muscle crossbridge including the actomyosin binding site which play important roles in the actomyosin crossbridge cycle. To distinguish… (more)

Subjects/Keywords: Actomyosin.; actomyosin; crossbridge; single molecule assay

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APA (6^th Edition):

Gundapaneni, D. (2004). Studies on actomyosin crossbridge flexibility using a new single molecule assay. (Thesis). University of North Texas. Retrieved from https://digital.library.unt.edu/ark:/67531/metadc4514/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Gundapaneni, Deepika. “Studies on actomyosin crossbridge flexibility using a new single molecule assay.” 2004. Thesis, University of North Texas. Accessed March 01, 2021. https://digital.library.unt.edu/ark:/67531/metadc4514/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Gundapaneni, Deepika. “Studies on actomyosin crossbridge flexibility using a new single molecule assay.” 2004. Web. 01 Mar 2021.

Vancouver:

Gundapaneni D. Studies on actomyosin crossbridge flexibility using a new single molecule assay. [Internet] [Thesis]. University of North Texas; 2004. [cited 2021 Mar 01]. Available from: https://digital.library.unt.edu/ark:/67531/metadc4514/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Gundapaneni D. Studies on actomyosin crossbridge flexibility using a new single molecule assay. [Thesis]. University of North Texas; 2004. Available from: https://digital.library.unt.edu/ark:/67531/metadc4514/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

5. Han, MKL. Cadherin mechanotransduction in morphogenesis: the importance of α-catenin and vinculin.

Degree: 2016, Universiteit Utrecht

URL: http://dspace.library.uu.nl:8080/handle/1874/334158

► The actomyosin contractile tension that is transmitted and regulated at cadherin-based cell-cell adhesions, greatly contributes to cell shape, cell migration, and coordinated tissue formation and… (more)

▼ The actomyosin contractile tension that is transmitted and regulated at cadherin-based cell-cell adhesions, greatly contributes to cell shape, cell migration, and coordinated tissue formation and organization. In a process termed mechanotransduction, the protein complexes in cell adhesions are able to sense changes in tension and induce biochemical feedback pathways to respond to these changes. The best understood mechanism is the force-induced recruitment of vinculin to α-catenin in cadherin junctions that leads to strengthening of the adhesion. However, the importance of this mechanism for the development of a living organism has not been directly studied yet. Here we investigated whether α-catenin/vinculin-dependent cadherin mechanotransduction is important for zebrafish development, and how changes in tension at E-cadherin cell-cell contacts might contribute to the total intercellular stress in a remodeling epithelium. We first generated α-catenin-deficient zebrafish using TALEN gene editing technology. We then specifically disrupted αE-catenin-dependent mechanotransduction while maintaining junction-forming structural capacity, by using an αE-catenin construct lacking the vinculin-binding domain (α-catenin-ΔVBS). αE-catenin mutant embryos show loss of epithelial integrity, which is restored upon expression of either wild-type α-catenin or α-catenin-ΔVBS. However, expression of α-catenin-ΔVBS also perturbed convergence and extension cell movements during gastrulation. Further investigation revealed that the vinculin binding domain of a-catenin is essential for cadherin-dependent morphogenetic cell movements in the developing zebrafish embryo. Next, we investigated the role of vinculin in zebrafish development directly by generating loss-of-function alleles using TALEN and CRISPR-Cas gene editing technologies. Surprisingly, we found that zygotic loss of the two functional vinculin genes present in zebrafish, did not cause observable defects in early development. However, vinculin A-vinculin B double mutants failed to survive until adulthood, suggesting that vinculin is not needed for the early development and morphogenesis of zebrafish tissues but becomes essential after embryonal stages. To study the relationship between tension on E-Cadherin and total intercellular tension during tissue remodeling in epithelial monolayers, we characterized a FRET biosensor which measures tension on E-Cadherin molecules in single junctions. Simultaneously, we measured the total intercellular tension in the same junctions using Monolayer Stress Microscopy, which calculates the total monolayer stress from the traction forces exerted by cell-ECM adhesions. We determined that the magnitude of E-Cadherin tension is not predictive of the total level of junctional tension. Instead, we find that E-Cadherin tension rapidly and proportionally changes with total junctional tension during tissue remodeling. This places E-cadherin at an ideal position for mechanically induced feedback signaling into junctional actin organization. In this… Advisors/Committee Members: Bos, Johannes L. (Hans), de Rooij, Johan.

Subjects/Keywords: Cadherin; α-catenin; vinculin; mechanotransduction; tension; zebrafish; morphogenesis; gastrulation; adhesion; actomyosin

11 more images…

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APA (6^th Edition):

Han, M. (2016). Cadherin mechanotransduction in morphogenesis: the importance of α-catenin and vinculin. (Doctoral Dissertation). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/334158

Chicago Manual of Style (16^th Edition):

Han, MKL. “Cadherin mechanotransduction in morphogenesis: the importance of α-catenin and vinculin.” 2016. Doctoral Dissertation, Universiteit Utrecht. Accessed March 01, 2021. http://dspace.library.uu.nl:8080/handle/1874/334158.

MLA Handbook (7^th Edition):

Han, MKL. “Cadherin mechanotransduction in morphogenesis: the importance of α-catenin and vinculin.” 2016. Web. 01 Mar 2021.

Vancouver:

Han M. Cadherin mechanotransduction in morphogenesis: the importance of α-catenin and vinculin. [Internet] [Doctoral dissertation]. Universiteit Utrecht; 2016. [cited 2021 Mar 01]. Available from: http://dspace.library.uu.nl:8080/handle/1874/334158.

Council of Science Editors:

Han M. Cadherin mechanotransduction in morphogenesis: the importance of α-catenin and vinculin. [Doctoral Dissertation]. Universiteit Utrecht; 2016. Available from: http://dspace.library.uu.nl:8080/handle/1874/334158

University of Illinois – Chicago

6. Shimizu, Yuka. The Role of nmMLCK in the Regulation of Lung Vascular Barrier Integrity During Pulmonary Inflammation.

Degree: 2015, University of Illinois – Chicago

URL: http://hdl.handle.net/10027/19401

► Increased vascular permeability and accumulation of alveolar fluid are cardinal features of lung inflammation such as acute lung injury (ALI), acute respiratory distress syndrome (ARDS),… (more)

▼ Increased vascular permeability and accumulation of alveolar fluid are cardinal features of lung inflammation such as acute lung injury (ALI), acute respiratory distress syndrome (ARDS), and asthma. Two key molecules implicated in the pathogenesis of these diseases are vascular endothelial growth factor (VEGF), a growth factor that promotes vascular permeability, and the non-muscle isoform of myosin light chain kinase (nmMLCK), a key regulator of vascular barrier function via actin-myosin mediated cell contraction. VEGF is a known factor upregulated in lung during ALI/ARDS and asthma pathogenesis. Also, we have recently demonstrated that nmMLCK mRNA and protein expression are upregulated in asthmatic patients and our previous work has shown that the variants in the gene encoding nmMLCK (MYLK) are associated with severe asthma in African descent individuals, strongly suggesting their involvement in asthmatic pathophysiology. In this study, I hypothesized that nmMLCK mediates VEGF induced vascular hyperpermeability in asthmatic inflammation and have provided strong evidence for a linkage between these two major asthmatic molecules. Using a combination of in vivo, in vitro, and in silico studies, I have demonstrated increased expression of nmMLCK in OVA experimental model of asthma, and this upregulation and activation of nmMLCK is mediated by VEGF. These data demonstrate that increased levels and activity of nmMLCK is strongly associated with asthmatic inflammation. Interestingly, VEGF stimulation significantly increases nmMLCK mRNA and protein expression, with Sp1 transcription factor binding and de-methylation of the nmMYLK promoter being likely mechanisms modulating nmMLCK expression. These mechanistic interactions between two major asthmatic molecules present potential novel candidates for therapeutic targeting to reduce asthmatic inflammation. Advisors/Committee Members: Ackerman, Steven J. (advisor), Garcia, Joe G. (committee member), Tyner, Angela L. (committee member), Simonovic, Miljan (committee member), Dudek, Steven M. (committee member), Yuan, Jason X. (committee member).

Subjects/Keywords: Vascular permeability; Pulmonary inflammation; Actomyosin contraction; nmMLCK; VEGF; Asthma

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APA (6^th Edition):

Shimizu, Y. (2015). The Role of nmMLCK in the Regulation of Lung Vascular Barrier Integrity During Pulmonary Inflammation. (Thesis). University of Illinois – Chicago. Retrieved from http://hdl.handle.net/10027/19401

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Shimizu, Yuka. “The Role of nmMLCK in the Regulation of Lung Vascular Barrier Integrity During Pulmonary Inflammation.” 2015. Thesis, University of Illinois – Chicago. Accessed March 01, 2021. http://hdl.handle.net/10027/19401.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Shimizu, Yuka. “The Role of nmMLCK in the Regulation of Lung Vascular Barrier Integrity During Pulmonary Inflammation.” 2015. Web. 01 Mar 2021.

Vancouver:

Shimizu Y. The Role of nmMLCK in the Regulation of Lung Vascular Barrier Integrity During Pulmonary Inflammation. [Internet] [Thesis]. University of Illinois – Chicago; 2015. [cited 2021 Mar 01]. Available from: http://hdl.handle.net/10027/19401.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Shimizu Y. The Role of nmMLCK in the Regulation of Lung Vascular Barrier Integrity During Pulmonary Inflammation. [Thesis]. University of Illinois – Chicago; 2015. Available from: http://hdl.handle.net/10027/19401

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Illinois – Chicago

7. Daneshjou, Nazila. Role of Rac1 in Regulating the Integrity of Endothelial Adherens Junctions.

Degree: 2015, University of Illinois – Chicago

URL: http://hdl.handle.net/10027/19411

► The endothelium forms a semi-permeable barrier that is critical for maintaining tissue fluid homeostasis. Endothelial permeability if mainly regulated via Adherens Junctions (AJs) complex. The… (more)

▼ The endothelium forms a semi-permeable barrier that is critical for maintaining tissue fluid homeostasis. Endothelial permeability if mainly regulated via Adherens Junctions (AJs) complex. The RhoGTPases Rac1 and RhoA play critical roles in regulating endothelial junctional permeability, however, the relationship between localized activity of RhoGTPases and the stability of Vascular Endothelial (VE)-cadherin adhesion, the main adhesive protein of AJs, remains unclear. Here using a photo-activatable probe to control spatiotemporal Rac1 activity at AJs, we addressed the relationship between Rac1 and dynamics of VE-cadherin in sub-confluent and confluent endothelium. We observed that in a sub-confluent monolayer Rac1 activation induced lamellipodia ruffling-a well-known effect of Rac1, and promoted gap sealing. Interestingly, Rac1 activation at mature junctions reduced the rate of VE-cadherin dissociation leading to increased density of VE-cadherin at AJs. This response was coupled to a reduction in RhoA-mediated and actomyosin-dependent tension across VE-cadherin adhesion sites and was independent of lamellipodia activity. Similar to Rac1 activation, inhibition of myosin II, downstream of RhoA, directly or through photo-release of the caged Rho kinase inhibitor also reduced the rate of VE-cadherin dissociation. Both, in silico modeling and kinetic analysis of VE-cadherin mutant in cells, established the inverse relationship between stability of adhesive bonds and the time of VE-cadherin retention at AJs. Thus, Rac1 functioned by stabilizing VE-cadherin adhesion in mature AJs by counteracting the actomyosin tension. The results suggest a new model of VE-cadherin adhesive interaction mediated by Rac1-induced reduction of mechanical tension at AJs resulting in the stabilizing of VE-cadherin adhesions. Advisors/Committee Members: Malik, Asrar B. (advisor), Komarova, Yulia A. (committee member), Mehta, Dolly (committee member), Karginov, Andrei (committee member), Gottardi, Cara (committee member).

Subjects/Keywords: Endothelium; Adherens Junctions; VE-cadherin; RhoGTPases; Actomyosin Tension

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APA (6^th Edition):

Daneshjou, N. (2015). Role of Rac1 in Regulating the Integrity of Endothelial Adherens Junctions. (Thesis). University of Illinois – Chicago. Retrieved from http://hdl.handle.net/10027/19411

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Daneshjou, Nazila. “Role of Rac1 in Regulating the Integrity of Endothelial Adherens Junctions.” 2015. Thesis, University of Illinois – Chicago. Accessed March 01, 2021. http://hdl.handle.net/10027/19411.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Daneshjou, Nazila. “Role of Rac1 in Regulating the Integrity of Endothelial Adherens Junctions.” 2015. Web. 01 Mar 2021.

Vancouver:

Daneshjou N. Role of Rac1 in Regulating the Integrity of Endothelial Adherens Junctions. [Internet] [Thesis]. University of Illinois – Chicago; 2015. [cited 2021 Mar 01]. Available from: http://hdl.handle.net/10027/19411.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Daneshjou N. Role of Rac1 in Regulating the Integrity of Endothelial Adherens Junctions. [Thesis]. University of Illinois – Chicago; 2015. Available from: http://hdl.handle.net/10027/19411

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universitat Pompeu Fabra

8. Hayes, D. Peran, 1983-. On the orchestration of dorsal closure in Drosophila melanogaster : from tissue-scale control to cellular-scale remodeling.

Degree: Departament de Ciències Experimentals i de la Salut, 2016, Universitat Pompeu Fabra

URL: http://hdl.handle.net/10803/586069

► La gran diversitat de vida a la terra es va desenvolupar arran d’uns inicis relativament uniformes i mitjançant moviments autoorganitzats que varen ocórrer entre grups… (more)

▼ La gran diversitat de vida a la terra es va desenvolupar arran d’uns inicis relativament uniformes i mitjançant moviments autoorganitzats que varen ocórrer entre grups de cèl·lules. Mentre que la varietat de formes que sorgeixen de la morfogènesi és il·limitada, el nombre de canvis geomètrics i topològics que la constitueixen és limitat. El control de la forma biològica sorgeix de la interacció entre la biologia molecular i la biomecànica. En aquest treball hem adoptat una aproximació biofísica per estudiar la regulació d’una transformació topològica que té lloc durant el desenvolupament de la mosca de la fruita Drosophila melanogaster. Just abans que l’embrió de Drosophila esdevingui en larva, el seu cos es cobreix amb un únic estrat de teixit epidèrmic. Per aconseguir-lo, dues capes d’epidermis s’estiren lateralment envoltant l’embrió i es fusionen dorsalment, segellant un locus que prèviament estava ple d’un teixit mort i pulsatiu anomenat amnioserosa (AS). Aquest procés es coneix com a "tancament dorsal" i resulta de gran interès com a model per estudiar altres esdeveniments de fusió tissular, com ara la cicatrització de ferides o el tancament del tub neural. Amb aquest projecte, en primer lloc fem una anàlisi del control global del procés, derivant una descripció biofísica del sistema a partir de principis bàsics primordials de la física: aquests principis són una força d’equilibri en els dos cantons (canthi) de l’obertura, i la relació Young-Laplace al voltant del seu contorn. Aquest model representa un avenç en relació a treballs previs com a conseqüència de la seva simplicitat, i la introducció d’un terme per a la pèrdua de volum de l’AS en una descripció de dues dimensions. En la segona part de la tesi, ens centrem en el paper que té el comportament pulsatiu de l’AS, ampliant el coneixement existent sobre un nou assaig experimental que pertorba mecànicament l’embrió de la mosca Drosophila. Combinant aquesta aproximació amb un nou mètode per extreure informa-ció quantitativa d’imatges confocals de les cèl·lules, investiguem la resposta de l’AS a l’estrès mecànic. Finalment, proposem una doble funció per als fluxos corticals de miosina a l’AS: donant suport estructural a les unions que s’estiren i promovent l’eliminació de l’excés d’unions mitjançant l’endocitosi Advisors/Committee Members: [email protected] (authoremail), true (authoremailshow), Solon, Jérôme (director), true (authorsendemail).

Subjects/Keywords: Dorsal Closure; Biomechanics; Morphogenesis; Actomyosin; Pulsation; Oscillation; Epithelia; Cadherin; Junction; 575

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APA (6^th Edition):

Hayes, D. Peran, 1. (2016). On the orchestration of dorsal closure in Drosophila melanogaster : from tissue-scale control to cellular-scale remodeling. (Thesis). Universitat Pompeu Fabra. Retrieved from http://hdl.handle.net/10803/586069

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Hayes, D. Peran, 1983-. “On the orchestration of dorsal closure in Drosophila melanogaster : from tissue-scale control to cellular-scale remodeling.” 2016. Thesis, Universitat Pompeu Fabra. Accessed March 01, 2021. http://hdl.handle.net/10803/586069.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Hayes, D. Peran, 1983-. “On the orchestration of dorsal closure in Drosophila melanogaster : from tissue-scale control to cellular-scale remodeling.” 2016. Web. 01 Mar 2021.

Vancouver:

Hayes, D. Peran 1. On the orchestration of dorsal closure in Drosophila melanogaster : from tissue-scale control to cellular-scale remodeling. [Internet] [Thesis]. Universitat Pompeu Fabra; 2016. [cited 2021 Mar 01]. Available from: http://hdl.handle.net/10803/586069.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hayes, D. Peran 1. On the orchestration of dorsal closure in Drosophila melanogaster : from tissue-scale control to cellular-scale remodeling. [Thesis]. Universitat Pompeu Fabra; 2016. Available from: http://hdl.handle.net/10803/586069

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of California – San Francisco

9. Naylor, Stephen Gordon. Temporal Regulation of Protein Assembly by Cdk1.

Degree: Biochemistry and Molecular Biology, 2013, University of California – San Francisco

URL: http://www.escholarship.org/uc/item/2q53f492

► Post-translational phosphorylation of proteins by cyclin-dependent kinases (Cdks) enables regulation of cell cycle events with a temporal and spatial precision that would be unattainable by… (more)

▼ Post-translational phosphorylation of proteins by cyclin-dependent kinases (Cdks) enables regulation of cell cycle events with a temporal and spatial precision that would be unattainable by control of protein synthesis and degradation alone. To understand how processes are regulated to operate reliably in every repetition of the cycle thus requires an understanding of where and when Cdk-dependent phosphates appear throughout the proteome, and the molecular effects of those phosphates on the proteins in question.Cytokinesis, the process by which one cell divides and becomes two, involves the ordered assembly of several protein populations at the site of cell division. In the budding yeast Saccharomyces cerevisiae, this occurs at the bud neck and has the end result of coordinating the contraction of an actomyosin ring with the synthesis of the primary septum, a cell wall precursor. The assembly process occurs during a period or proteome-wide reversal of Cdk-dependent phosphorylation, and is known to be sensitive to perturbations of the Cdk regulatory system.This study focuses on Iqg1, an essential protein required for recruiting actin to the bud neck. Iqg1 is phosphorylated by Cdk1 (S. cerevisiae's sole essential Cdk) at several sites, and we find that mutation of these sites to yield a nonphosphorylatable Iqg1 disrupts the timing of several aspects of the pre-cytokinesis assembly process. When expressed in place of wild type Iqg1, the phosphomutant promotes early arrival of filamentous actin at the bud neck at levels comparable to those observed in response to global inhibition of Cdk1 activity. In this mutant, we also observe early onset of the accumulation of Iqg1 itself, and of Hof1, a regulator of primary septum deposition that we reveal as a likely Iqg1 in vivo binding partner.These results reveal a common point of regulation for the two processes that comprise cytokinesis and illuminate the relationships between proteins that govern the timing of assembly of a complex multi-protein apparatus. The quantitative fluorescence microscopy methods used also provide a system for the further study of the unique molecular consequences of phosphorylation for each of Cdk1's many protein substrates.

Subjects/Keywords: Cellular biology; actomyosin; Cdk1; cell cycle; cytokinesis; Iqg1; microscopy

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APA (6^th Edition):

Naylor, S. G. (2013). Temporal Regulation of Protein Assembly by Cdk1. (Thesis). University of California – San Francisco. Retrieved from http://www.escholarship.org/uc/item/2q53f492

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Naylor, Stephen Gordon. “Temporal Regulation of Protein Assembly by Cdk1.” 2013. Thesis, University of California – San Francisco. Accessed March 01, 2021. http://www.escholarship.org/uc/item/2q53f492.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Naylor, Stephen Gordon. “Temporal Regulation of Protein Assembly by Cdk1.” 2013. Web. 01 Mar 2021.

Vancouver:

Naylor SG. Temporal Regulation of Protein Assembly by Cdk1. [Internet] [Thesis]. University of California – San Francisco; 2013. [cited 2021 Mar 01]. Available from: http://www.escholarship.org/uc/item/2q53f492.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Naylor SG. Temporal Regulation of Protein Assembly by Cdk1. [Thesis]. University of California – San Francisco; 2013. Available from: http://www.escholarship.org/uc/item/2q53f492

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Toronto

10. Zheng, Shiyu. An Arf-GEF Pathway for Regulating Actomyosin at Adherens Junctions: the Role of an Adaptor Protein.

Degree: 2018, University of Toronto

URL: http://hdl.handle.net/1807/102813

►

Cytoskeletal regulation is essential for morphogenesis during development. One down-regulator of actomyosin in the Drosophila embryo is the Arf-GEF Steppke. I investigated how Steppke is… (more)

Subjects/Keywords: Actomyosin; Development; Dorsal Closure; Drosophila; Stepping stone; Steppke; 0758

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APA (6^th Edition):

Zheng, S. (2018). An Arf-GEF Pathway for Regulating Actomyosin at Adherens Junctions: the Role of an Adaptor Protein. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/102813

Chicago Manual of Style (16^th Edition):

Zheng, Shiyu. “An Arf-GEF Pathway for Regulating Actomyosin at Adherens Junctions: the Role of an Adaptor Protein.” 2018. Masters Thesis, University of Toronto. Accessed March 01, 2021. http://hdl.handle.net/1807/102813.

MLA Handbook (7^th Edition):

Zheng, Shiyu. “An Arf-GEF Pathway for Regulating Actomyosin at Adherens Junctions: the Role of an Adaptor Protein.” 2018. Web. 01 Mar 2021.

Vancouver:

Zheng S. An Arf-GEF Pathway for Regulating Actomyosin at Adherens Junctions: the Role of an Adaptor Protein. [Internet] [Masters thesis]. University of Toronto; 2018. [cited 2021 Mar 01]. Available from: http://hdl.handle.net/1807/102813.

Council of Science Editors:

Zheng S. An Arf-GEF Pathway for Regulating Actomyosin at Adherens Junctions: the Role of an Adaptor Protein. [Masters Thesis]. University of Toronto; 2018. Available from: http://hdl.handle.net/1807/102813

University of Sydney

11. Nino, Jorge Luis Galeano. Cytoskeletal dynamics of Cytotoxic T cells during migration in the tumour microenvironment .

Degree: 2015, University of Sydney

URL: http://hdl.handle.net/2123/13608

► Typically, migrating T cells display an elongated polarized shape with a very dynamic leading edge and a uropod in the rear. This ‘amoeboid’ movement guarantees… (more)

Subjects/Keywords: actomyosin cortex; TGF-β; T cell migration; tumor microenvironment

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APA (6^th Edition):

Nino, J. L. G. (2015). Cytoskeletal dynamics of Cytotoxic T cells during migration in the tumour microenvironment . (Thesis). University of Sydney. Retrieved from http://hdl.handle.net/2123/13608

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Nino, Jorge Luis Galeano. “Cytoskeletal dynamics of Cytotoxic T cells during migration in the tumour microenvironment .” 2015. Thesis, University of Sydney. Accessed March 01, 2021. http://hdl.handle.net/2123/13608.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Nino, Jorge Luis Galeano. “Cytoskeletal dynamics of Cytotoxic T cells during migration in the tumour microenvironment .” 2015. Web. 01 Mar 2021.

Vancouver:

Nino JLG. Cytoskeletal dynamics of Cytotoxic T cells during migration in the tumour microenvironment . [Internet] [Thesis]. University of Sydney; 2015. [cited 2021 Mar 01]. Available from: http://hdl.handle.net/2123/13608.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Nino JLG. Cytoskeletal dynamics of Cytotoxic T cells during migration in the tumour microenvironment . [Thesis]. University of Sydney; 2015. Available from: http://hdl.handle.net/2123/13608

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

UCLA

12. Katz, Sophie Sabine. Forming a Worm: The Role of the Epidermal Cytoskeleton and the Extracellular Matrix in Shaping the Cuticle of C. elegans.

Degree: Biological Chemistry, 2018, UCLA

URL: http://www.escholarship.org/uc/item/6td6b4nc

► Understanding how cells respond to and remodel their extracellular environment is foundational to morphogenesis. Intricate apical or luminal matrices are vital components of numerous epithelial… (more)

▼ Understanding how cells respond to and remodel their extracellular environment is foundational to morphogenesis. Intricate apical or luminal matrices are vital components of numerous epithelial organs and yet shaped by elusive mechanisms. In my thesis research, I have used the molting cycle in the nematode C. elegans to study how the actin cytoskeleton is coupled to the extracellular matrix and functions as a key effector of cellular behavior. In this thesis, I characterize the mechanical networks and successive changes in tissue morphology that give rise to long ridges called alae on certain cuticles of C.�elegans. We show that knockdowns of apical constriction components lead to maze-like cuticle deformities overlying lateral epidermal syncytia (seam). Transient apical constriction leads to the formation of three actin filament bundles in the seam and adjacent syncytia that presage the forthcoming ridges of the alae. The rapid constriction and gradual expansion of the epidermis generates transient protuberances from the surface that organize provisional matrix macromolecules. The provisional matrix enables power from apical constriction to shape durable substructures in apical extracellular matrix. The proposed mechanism integrates changes in cell and extracellular matrix morphology over time and might apply to significant developmental transitions in other systems. I also reveal the extensive dynamic rearrangements of the epidermal actin cytoskeleton over the course of the last larval stage and molt. My findings show that the propagation of annular folds from larval to adult-stage cuticles does not rely on a subset of circumferential actin filament bundles present at the cortex of the major epidermal syncytium across the larval-to-adult transition. This leads to a revised understanding of patterning in which matrix proteins themselves shape the cuticle and regulate the organization of the transient molting sheath as well as the actin cytoskeleton with the epidermis. Lastly, I present preliminary evidence of regulators of the actin cytoskeleton within the epidermis, which may act downstream of the matrix.

Subjects/Keywords: Cellular biology; Developmental biology; Molecular biology; actomyosin cytoskeleton; c elegans; cuticle; molting; morphogenesis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Katz, S. S. (2018). Forming a Worm: The Role of the Epidermal Cytoskeleton and the Extracellular Matrix in Shaping the Cuticle of C. elegans. (Thesis). UCLA. Retrieved from http://www.escholarship.org/uc/item/6td6b4nc

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Katz, Sophie Sabine. “Forming a Worm: The Role of the Epidermal Cytoskeleton and the Extracellular Matrix in Shaping the Cuticle of C. elegans.” 2018. Thesis, UCLA. Accessed March 01, 2021. http://www.escholarship.org/uc/item/6td6b4nc.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Katz, Sophie Sabine. “Forming a Worm: The Role of the Epidermal Cytoskeleton and the Extracellular Matrix in Shaping the Cuticle of C. elegans.” 2018. Web. 01 Mar 2021.

Vancouver:

Katz SS. Forming a Worm: The Role of the Epidermal Cytoskeleton and the Extracellular Matrix in Shaping the Cuticle of C. elegans. [Internet] [Thesis]. UCLA; 2018. [cited 2021 Mar 01]. Available from: http://www.escholarship.org/uc/item/6td6b4nc.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Katz SS. Forming a Worm: The Role of the Epidermal Cytoskeleton and the Extracellular Matrix in Shaping the Cuticle of C. elegans. [Thesis]. UCLA; 2018. Available from: http://www.escholarship.org/uc/item/6td6b4nc

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Cornell University

13. Brown, Joel. INVESTIGATING MAMMALIAN DEVELOPMENT USING MOUSE FORWARD GENETICS.

Degree: PhD, Genetics and Development, 2018, Cornell University

URL: http://hdl.handle.net/1813/59291

► Forward genetics allows the identification of novel genes involved in a particular biological process. We performed an ENU forward mutagenesis screen in mice aimed at… (more)

▼ Forward genetics allows the identification of novel genes involved in a particular biological process. We performed an ENU forward mutagenesis screen in mice aimed at identifying genes which regulate early organ morphogenesis during development. Fifteen mouse mutants were identified in the screen which possess cardiovascular, craniofacial, extraembryonic, or general body morphology defects. After performing preliminary characterization of all 15 lines, we identified a likely causative mutation in 14 of the 15 mutants. 1D and 13B mutants both exhibit craniofacial defects and were selected for in-depth characterization to identify the molecular mechanisms whereby they regulate embryogenesis. 1D mutants are characterized by an open neural tube in the hindbrain region. This phenotype is similar to human embryos with exencephaly, a congenital birth malformation of high incidence in the human population. Positional cloning of 1D identified a mutation in SPCA1, a Golgi-localized pump that controls calcium homeostasis. Results from the molecular characterization of mouse 1D mutants, as well as from time lapse microscopy of chicken embryos, revealed that calcium is tightly regulated during neural tube closure and that calcium homeostasis is required to promote apical constriction of neuroepithelial cells. These results show that SPCA1 activity is required to regulate the actomyosin dynamics that propel apical constriction, and that the actin severing protein, Cofilin 1, is a key mediator of SPCA1 function. Together, my findings provide the first genetic evidence that calcium homeostasis is needed for neural tube closure, opening a new window into understanding the etiology of human neural tube defects. 13B mutants have neural tube defects accompanied by a suite of other malformations including randomized L-R patterning and maxillary overgrowth. Molecular characterization revealed that all 13B phenotypes result from the absence of cilia, an organelle important for neural tube patterning and for the establishment of L-R asymmetry. Exome sequencing of 13B embryos identified a nonsense mutation in Pibf1. I show that PIBF1 is required for ciliogenesis during early embryonic development and identify a novel role for PIBF1 in regulating centrosome duplication. These findings highlight the importance of PIBF1 in regulating multiple aspects of centrosome biology, and provide a model for understanding how defects in these processes contribute to human ciliopathies. Advisors/Committee Members: Garcia-Garcia, Maria J. (chair), Schimenti, John C. (committee member), Kurpios, Natasza (committee member).

Subjects/Keywords: calcium; Actomyosin; Apical Constriction; Cofilin 1; Neural Tube; SPCA1; Developmental biology; Genetics; Molecular biology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Brown, J. (2018). INVESTIGATING MAMMALIAN DEVELOPMENT USING MOUSE FORWARD GENETICS. (Doctoral Dissertation). Cornell University. Retrieved from http://hdl.handle.net/1813/59291

Chicago Manual of Style (16^th Edition):

Brown, Joel. “INVESTIGATING MAMMALIAN DEVELOPMENT USING MOUSE FORWARD GENETICS.” 2018. Doctoral Dissertation, Cornell University. Accessed March 01, 2021. http://hdl.handle.net/1813/59291.

MLA Handbook (7^th Edition):

Brown, Joel. “INVESTIGATING MAMMALIAN DEVELOPMENT USING MOUSE FORWARD GENETICS.” 2018. Web. 01 Mar 2021.

Vancouver:

Brown J. INVESTIGATING MAMMALIAN DEVELOPMENT USING MOUSE FORWARD GENETICS. [Internet] [Doctoral dissertation]. Cornell University; 2018. [cited 2021 Mar 01]. Available from: http://hdl.handle.net/1813/59291.

Council of Science Editors:

Brown J. INVESTIGATING MAMMALIAN DEVELOPMENT USING MOUSE FORWARD GENETICS. [Doctoral Dissertation]. Cornell University; 2018. Available from: http://hdl.handle.net/1813/59291

Texas Medical Center

14. Yang, Chih-Chao. ADHERENS JUNCTIONS AND THE ACTOMYOSIN NETWORK REGULATE ORGAN GROWTH BY MODULATING HIPPO PATHWAY ACTIVITY IN DROSOPHILA.

Degree: PhD, 2012, Texas Medical Center

URL: https://digitalcommons.library.tmc.edu/utgsbs_dissertations/287

► Adherens junctions (AJs) and basolateral modules are important for the establishment and maintenance of apico-basal polarity. Loss of AJs and basolateral module members lead… (more)

▼ Adherens junctions (AJs) and basolateral modules are important for the establishment and maintenance of apico-basal polarity. Loss of AJs and basolateral module members lead to tumor formation, as well as poor prognosis for metastasis. Recently, in mammalian studies it has been shown that loss of either AJ or basolateral module members deregulate Yorkie activity, the downstream transcriptional effector of the Hippo pathway. Importantly, it is unclear if AJ and basolateral components act through the same or parallel mechanisms to regulate Yorkie activity. Here, we dissect how loss of AJ and basolateral components affects Hippo signaling in Drosophila. Surprisingly, while scrib knock-down tissue displays increased reporter activity autonomously, α-cat knock-down tissue shows a cell autonomous decrease and a cell non-autonomous increase of Hippo reporter activity. We provided several lines of evidence to show the differential regulation in polarity protein localizations and oncogenic cooperative overgrowth by AJs and basolateral complexes. Finally, we show that Hippo pathway activity is induced in α-cat and scrib double knocked-down tissue. Taken together, our results provide evidence to show that basolateral modules and AJs act in parallel to modulate Hippo pathway activity. Non-muscle myosin II is an actomyosin component that interacts with the actin. Non-muscle myosin II also interacts with lgl, though the function of this interaction is not clear. Our lab demonstrated that modulating F-actin regulates Hippo pathway activity, and lgl also has been described as a Hippo pathway regulator. Therefore we suspect that myosin II is also involved in Hippo pathway regulation. We first characterized non-muscle Myosin II as a novel tumor suppressor gene by affecting Hippo pathway activity. Upstream regulators of Myosin II, members in the Rho signaling pathway, also displayed similar phenotypes as the Myosin II knock-down tissues. Apoptosis is also induced in myosin II knock-down tissues, however, blocking cell death does not affect myosin II knock-down induced Hippo activation. Our data suggested hyperactivating myosin II induced F-actin accumulation so therefore induces Hippo target activation. Unexpectedly, we also observed that reducing F-actin activity induced Hippo target activation in vivo. These controversial data indicated that actomyosin may regulate the Hippo pathway through multiple mechanisms. Advisors/Committee Members: Halder, Georg, Galko, Michael, Jafar-Nejad, Hamed.

Subjects/Keywords: Hippo pathways; adherens junctions; polarity; growth control; actomyosin; cytoskeleton; Cell and Developmental Biology

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APA (6^th Edition):

Yang, C. (2012). ADHERENS JUNCTIONS AND THE ACTOMYOSIN NETWORK REGULATE ORGAN GROWTH BY MODULATING HIPPO PATHWAY ACTIVITY IN DROSOPHILA. (Doctoral Dissertation). Texas Medical Center. Retrieved from https://digitalcommons.library.tmc.edu/utgsbs_dissertations/287

Chicago Manual of Style (16^th Edition):

Yang, Chih-Chao. “ADHERENS JUNCTIONS AND THE ACTOMYOSIN NETWORK REGULATE ORGAN GROWTH BY MODULATING HIPPO PATHWAY ACTIVITY IN DROSOPHILA.” 2012. Doctoral Dissertation, Texas Medical Center. Accessed March 01, 2021. https://digitalcommons.library.tmc.edu/utgsbs_dissertations/287.

MLA Handbook (7^th Edition):

Yang, Chih-Chao. “ADHERENS JUNCTIONS AND THE ACTOMYOSIN NETWORK REGULATE ORGAN GROWTH BY MODULATING HIPPO PATHWAY ACTIVITY IN DROSOPHILA.” 2012. Web. 01 Mar 2021.

Vancouver:

Yang C. ADHERENS JUNCTIONS AND THE ACTOMYOSIN NETWORK REGULATE ORGAN GROWTH BY MODULATING HIPPO PATHWAY ACTIVITY IN DROSOPHILA. [Internet] [Doctoral dissertation]. Texas Medical Center; 2012. [cited 2021 Mar 01]. Available from: https://digitalcommons.library.tmc.edu/utgsbs_dissertations/287.

Council of Science Editors:

Yang C. ADHERENS JUNCTIONS AND THE ACTOMYOSIN NETWORK REGULATE ORGAN GROWTH BY MODULATING HIPPO PATHWAY ACTIVITY IN DROSOPHILA. [Doctoral Dissertation]. Texas Medical Center; 2012. Available from: https://digitalcommons.library.tmc.edu/utgsbs_dissertations/287

Northeastern University

15. Bouffard, Jeff. Development Of A Lab Bioimage Informatics System For Fluorescence Microscopy Data, With Application To Experimental Studies Of Rhogap Regulation Of Calcium Signaling And Actomyosin Contractility.

Degree: PhD, Department of Bioengineering, 2019, Northeastern University

URL: http://hdl.handle.net/2047/D20328157

► Microscopic imaging is a powerful tool to advance our understanding of biological systems. Image-based biological investigations can be large and complex, requiring bioimage informatics solutions… (more)

▼ Microscopic imaging is a powerful tool to advance our understanding of biological systems. Image-based biological investigations can be large and complex, requiring bioimage informatics solutions to manage and examine large amounts of information. This dissertation describes the development of a lab bioimage informatics system to organize and analyze fluorescence microscopy movies. This system is applied here to investigate the regulation of calcium signaling and actomyosin contractility in the C. elegans spermatheca, a multicellular contractile tube with stereotyped tissue function and conserved genes and regulatory networks. The lab bioimage informatics system standardizes the organization, processing, and analysis of calcium sensor movies by using computer programs to automate processes. The motivations, design goals, and implementation for this lab bioimage informatics system are presented. The experimental investigation revealed a new role for a RhoGAP known to regulate actomyosin contractility. For this work, the lab bioimage informatics system supported analysis of almost 500 fluorescence microscopy movies, acquired by 4 different people over 4 years. These data show that the RhoGAP SPV-1 is a key regulator of calcium signaling and tissue function in the C. elegans spermatheca. Experiments to establish mechanism suggest SPV-1 coordinates the activity of multiple GTPases to control tissue contractility. An additional prototype image processing and analysis pipeline is presented, which automatically segments the spermathecal tissue from calcium sensor movies. This pipeline will advance spatial analysis of the tissue, enabling quantitative analysis of tissue shape changes and mechanochemical signaling in the spermatheca. A major product of this dissertation is an operational lab bioimage informatics system, used by multiple researchers for more than two and a half years to organize movies and support publications and collaborations. This dissertation work also produced a biological investigation, using standardized analysis of movies, which was published in a Molecular Biology of the Cell special issue on Quantitative Cell Biology. A final product is an image processing pipeline that extracts new spatial measurements from movies. The tools and insights presented here continue to be used, and the framework presented may be useful for other researchers dealing with large amounts of complex imaging data.

Subjects/Keywords: actomyosin contractlity; bioimage informatics; calcium signaling; fluorescence microscopy; image analysis; RhoGAP; Bioengineering; Cellular biology; Bioinformatics

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APA (6^th Edition):

Bouffard, J. (2019). Development Of A Lab Bioimage Informatics System For Fluorescence Microscopy Data, With Application To Experimental Studies Of Rhogap Regulation Of Calcium Signaling And Actomyosin Contractility. (Doctoral Dissertation). Northeastern University. Retrieved from http://hdl.handle.net/2047/D20328157

Chicago Manual of Style (16^th Edition):

Bouffard, Jeff. “Development Of A Lab Bioimage Informatics System For Fluorescence Microscopy Data, With Application To Experimental Studies Of Rhogap Regulation Of Calcium Signaling And Actomyosin Contractility.” 2019. Doctoral Dissertation, Northeastern University. Accessed March 01, 2021. http://hdl.handle.net/2047/D20328157.

MLA Handbook (7^th Edition):

Bouffard, Jeff. “Development Of A Lab Bioimage Informatics System For Fluorescence Microscopy Data, With Application To Experimental Studies Of Rhogap Regulation Of Calcium Signaling And Actomyosin Contractility.” 2019. Web. 01 Mar 2021.

Vancouver:

Bouffard J. Development Of A Lab Bioimage Informatics System For Fluorescence Microscopy Data, With Application To Experimental Studies Of Rhogap Regulation Of Calcium Signaling And Actomyosin Contractility. [Internet] [Doctoral dissertation]. Northeastern University; 2019. [cited 2021 Mar 01]. Available from: http://hdl.handle.net/2047/D20328157.

Council of Science Editors:

Bouffard J. Development Of A Lab Bioimage Informatics System For Fluorescence Microscopy Data, With Application To Experimental Studies Of Rhogap Regulation Of Calcium Signaling And Actomyosin Contractility. [Doctoral Dissertation]. Northeastern University; 2019. Available from: http://hdl.handle.net/2047/D20328157

Universitat Pompeu Fabra

16. Czerniak, Natalia Dorotea, 1985-. Head involution in Drosophila melanogaster: on the role of supracellular actomyosin structures in tissue bending, spreading, and positioning.

Degree: Departament de Ciències Experimentals i de la Salut, 2015, Universitat Pompeu Fabra

URL: http://hdl.handle.net/10803/386441

► La morfogènesi crea una plètora de formes complexes en animals i plantes. Hem consagrat aquest treball a l'estudi de la involució del cap (head involution… (more)

Subjects/Keywords: Morphogenesis; Head involution; Epidermis; Hedgehog; Actomyosin cable; Involució del cap; Cable d'actomyosina; Morfogènesi; 575

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APA (6^th Edition):

Czerniak, Natalia Dorotea, 1. (2015). Head involution in Drosophila melanogaster: on the role of supracellular actomyosin structures in tissue bending, spreading, and positioning. (Thesis). Universitat Pompeu Fabra. Retrieved from http://hdl.handle.net/10803/386441

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Czerniak, Natalia Dorotea, 1985-. “Head involution in Drosophila melanogaster: on the role of supracellular actomyosin structures in tissue bending, spreading, and positioning.” 2015. Thesis, Universitat Pompeu Fabra. Accessed March 01, 2021. http://hdl.handle.net/10803/386441.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Czerniak, Natalia Dorotea, 1985-. “Head involution in Drosophila melanogaster: on the role of supracellular actomyosin structures in tissue bending, spreading, and positioning.” 2015. Web. 01 Mar 2021.

Vancouver:

Czerniak, Natalia Dorotea 1. Head involution in Drosophila melanogaster: on the role of supracellular actomyosin structures in tissue bending, spreading, and positioning. [Internet] [Thesis]. Universitat Pompeu Fabra; 2015. [cited 2021 Mar 01]. Available from: http://hdl.handle.net/10803/386441.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Czerniak, Natalia Dorotea 1. Head involution in Drosophila melanogaster: on the role of supracellular actomyosin structures in tissue bending, spreading, and positioning. [Thesis]. Universitat Pompeu Fabra; 2015. Available from: http://hdl.handle.net/10803/386441

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Toronto

17. Bhatti, Amad Imran. PKCδ Regulates Actomyosin Organization in Leading Cellular Protrusions during Drosophila Border Cell Migration.

Degree: 2020, University of Toronto

URL: http://hdl.handle.net/1807/103539

►

Actin-based membrane protrusions are essential for cell migration, including the collective migration of the border cell cluster (BCC) during Drosophila oogenesis. Our lab previously showed… (more)

Subjects/Keywords: Actomyosin dynamics; Border cell cluster; Cellular protrusions; Collective cell migration; Confocal microscopy; PKCδ; 0379

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APA (6^th Edition):

Bhatti, A. I. (2020). PKCδ Regulates Actomyosin Organization in Leading Cellular Protrusions during Drosophila Border Cell Migration. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/103539

Chicago Manual of Style (16^th Edition):

Bhatti, Amad Imran. “PKCδ Regulates Actomyosin Organization in Leading Cellular Protrusions during Drosophila Border Cell Migration.” 2020. Masters Thesis, University of Toronto. Accessed March 01, 2021. http://hdl.handle.net/1807/103539.

MLA Handbook (7^th Edition):

Bhatti, Amad Imran. “PKCδ Regulates Actomyosin Organization in Leading Cellular Protrusions during Drosophila Border Cell Migration.” 2020. Web. 01 Mar 2021.

Vancouver:

Bhatti AI. PKCδ Regulates Actomyosin Organization in Leading Cellular Protrusions during Drosophila Border Cell Migration. [Internet] [Masters thesis]. University of Toronto; 2020. [cited 2021 Mar 01]. Available from: http://hdl.handle.net/1807/103539.

Council of Science Editors:

Bhatti AI. PKCδ Regulates Actomyosin Organization in Leading Cellular Protrusions during Drosophila Border Cell Migration. [Masters Thesis]. University of Toronto; 2020. Available from: http://hdl.handle.net/1807/103539

University of Toronto

18. Kobb, Anna. Actomyosin Dynamics During Coordinated Cell Movements in Embryonic Wound Repair.

Degree: PhD, 2019, University of Toronto

URL: http://hdl.handle.net/1807/99065

►

Embryos repair epithelial wounds rapidly, in a process driven by collective cell behaviors. Upon wounding, filamentous actin and the motor protein non-muscle myosin II polarize… (more)

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Embryos repair epithelial wounds rapidly, in a process driven by collective cell behaviors. Upon wounding, filamentous actin and the motor protein non-muscle myosin II polarize in the cells adjacent to the wound, forming a supracellular cable around the lesion. Contraction at the wound margin coordinates cell movements and drives rapid wound closure. Using FRAP in Drosophila embryos, we found that myosin turnover at the wound margin was slower than in actomyosin networks with reduced contractility. Using laser ablation we showed that tension in the wound margin cable increased as wound closure progressed, which was associated with reduced myosin turnover. Reducing tension resulted in increased turnover and loss of myosin from the wound edge. Finally, myosin motor activity was necessary for its stabilization around the wound and for efficient wound closure. Our results indicate that mechanical forces regulate myosin dynamics during embryonic wound repair, however we could not discount regulation of myosin through its binding partner, actin. To investigate a role for tension in regulating actin during wound repair, we used FRAP to show that actin was stabilized around embryonic wounds. Loss of tension through laser ablation led to loss of actin fluorescence, however, photobleaching experiments after laser severing showed no change in actin turnover. We found no changes in actin dynamics between early and late wound closure, when tension is higher. Together these data suggest that tension may be partially necessary for actin localization, but it is neither necessary nor sufficient to regulate actin turnover. To begin to pick apart the relationship between actin and myosin at the wound margin, we inhibited myosin activity and measured actin turnover. Actin stabilization was independent of myosin activity, suggesting actin may be regulated independently. To understand whether myosin turnover is independent of actin, we stabilized actin networks pharmacologically and monitored for changes in myosin dynamics. Myosin turnover was unaffected by actin stabilization. These results suggest that actin and myosin are independently regulated, and that these networks are more complex than expected. This work provides insights into the mechanisms used by cells to coordinate their behaviour and could have implications for development and disease.

2020-01-21 00:00:00

Advisors/Committee Members: Fernandez-Gonzalez, Rodrigo, Biomedical Engineering.

Subjects/Keywords: actomyosin contractility; collective migration; FRAP; fruit fly; Laser ablation; wound repair; 0541

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kobb, A. (2019). Actomyosin Dynamics During Coordinated Cell Movements in Embryonic Wound Repair. (Doctoral Dissertation). University of Toronto. Retrieved from http://hdl.handle.net/1807/99065

Chicago Manual of Style (16^th Edition):

Kobb, Anna. “Actomyosin Dynamics During Coordinated Cell Movements in Embryonic Wound Repair.” 2019. Doctoral Dissertation, University of Toronto. Accessed March 01, 2021. http://hdl.handle.net/1807/99065.

MLA Handbook (7^th Edition):

Kobb, Anna. “Actomyosin Dynamics During Coordinated Cell Movements in Embryonic Wound Repair.” 2019. Web. 01 Mar 2021.

Vancouver:

Kobb A. Actomyosin Dynamics During Coordinated Cell Movements in Embryonic Wound Repair. [Internet] [Doctoral dissertation]. University of Toronto; 2019. [cited 2021 Mar 01]. Available from: http://hdl.handle.net/1807/99065.

Council of Science Editors:

Kobb A. Actomyosin Dynamics During Coordinated Cell Movements in Embryonic Wound Repair. [Doctoral Dissertation]. University of Toronto; 2019. Available from: http://hdl.handle.net/1807/99065

Washington University in St. Louis

19. Loza, Andrew J. Structure, Dynamics, and Regulation of Collective Cell Migration.

Degree: PhD, Biology & Biomedical Sciences (Computational & Molecular Biophysics), 2016, Washington University in St. Louis

URL: https://openscholarship.wustl.edu/art_sci_etds/997

► Collective migration is the process by which cells organize individual motions to productively migrate as a group and plays a fundamental role in organism development,… (more)

▼ Collective migration is the process by which cells organize individual motions to productively migrate as a group and plays a fundamental role in organism development, tissue regeneration, and cancer invasion. In development, coordinated migration facilitates the formation of complex organ structures and is required for proper dissemination of neural crest cells throughout an organism. After injury, this process allows breaches in epithelial layers to be repaired while maintaining tissue integrity, and in cancer, collective behavior enhances invasion of tumor cells into the surrounding tissue. Chapter 1 provides an introduction for the role of collective migration across an organism’s lifespan, the mechanisms used by cells to generate motile force, and the emergence of collective behavior. Chapter 2 dissects the intertwined roles of three fundamental parameters often altered in collective migration processes: cell density, cell adhesion, and cell-cell contractility through the Rho-ROCK-Myosin II signaling axis. Through quantitative analysis of large-scale time-lapse imaging and mathematical modeling, I identify force-sensitive contractility and cell packing as mediators of two distinct classes of collective migration. From these results, I formulate a phase-diagram of collective cell migration and test predictions in an in-vivo epithelium using genetic manipulations to drive collective motion between predicted migratory phases. In Chapter 3, the effect of phenotypic heterogeneity on the organization of cells is examined, providing insight into the effects of early cancer progression on epithelial dynamics. I find that mutant cells within an otherwise wild-type tissue impact organization through local and field-effects, disrupting normal dynamics and leading to cell-type segregation. Chapter 4 provides a theoretical framework for quantitatively understanding and predicting the dynamics of protein interactions underlying biological processes including collective migration. Traditional chemical kinetics approaches break down in situations where components are slow diffusing or in countable numbers, requiring the formulation of new models that take into account this level of complexity. Here I develop an event-driven algorithm that bridges well-mixed and unmixed systems and use it to predict the effect of apparent changes in enzymatic efficiency due to alterations in mobility that may be caused by protein complex formation. Overall the work in this dissertation advances our understanding of the structure and dynamics of collective migration and the parameters governing this process by combining quantitative statistical analysis, mathematical modeling, and in-vivo live imaging. Advisors/Committee Members: Gregory D. Longmore, Elliot Elson, Guy Genin, Robert Mecham, Amit Pathak.

Subjects/Keywords: actomyosin contractility; cell adhesion; cell density; collective migration; phase diagram; spatiotemporal correlation

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APA (6^th Edition):

Loza, A. J. (2016). Structure, Dynamics, and Regulation of Collective Cell Migration. (Doctoral Dissertation). Washington University in St. Louis. Retrieved from https://openscholarship.wustl.edu/art_sci_etds/997

Chicago Manual of Style (16^th Edition):

Loza, Andrew J. “Structure, Dynamics, and Regulation of Collective Cell Migration.” 2016. Doctoral Dissertation, Washington University in St. Louis. Accessed March 01, 2021. https://openscholarship.wustl.edu/art_sci_etds/997.

MLA Handbook (7^th Edition):

Loza, Andrew J. “Structure, Dynamics, and Regulation of Collective Cell Migration.” 2016. Web. 01 Mar 2021.

Vancouver:

Loza AJ. Structure, Dynamics, and Regulation of Collective Cell Migration. [Internet] [Doctoral dissertation]. Washington University in St. Louis; 2016. [cited 2021 Mar 01]. Available from: https://openscholarship.wustl.edu/art_sci_etds/997.

Council of Science Editors:

Loza AJ. Structure, Dynamics, and Regulation of Collective Cell Migration. [Doctoral Dissertation]. Washington University in St. Louis; 2016. Available from: https://openscholarship.wustl.edu/art_sci_etds/997

Duke University

20. Hunter, Ginger. The Role of Mechanically Gated Ion Channels in Dorsal Closure During Drosophila Morphogenesis .

Degree: 2012, Duke University

URL: http://hdl.handle.net/10161/5601

► Physical forces play a key role in the morphogenesis of embryos. As cells and tissues change shape, grow, and migrate, they exert and respond… (more)

Subjects/Keywords: Developmental biology; Cellular biology; actomyosin; dorsal closure; drosophila; mechanically gated ion channels; mechanotransduction

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APA (6^th Edition):

Hunter, G. (2012). The Role of Mechanically Gated Ion Channels in Dorsal Closure During Drosophila Morphogenesis . (Thesis). Duke University. Retrieved from http://hdl.handle.net/10161/5601

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Hunter, Ginger. “The Role of Mechanically Gated Ion Channels in Dorsal Closure During Drosophila Morphogenesis .” 2012. Thesis, Duke University. Accessed March 01, 2021. http://hdl.handle.net/10161/5601.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Hunter, Ginger. “The Role of Mechanically Gated Ion Channels in Dorsal Closure During Drosophila Morphogenesis .” 2012. Web. 01 Mar 2021.

Vancouver:

Hunter G. The Role of Mechanically Gated Ion Channels in Dorsal Closure During Drosophila Morphogenesis . [Internet] [Thesis]. Duke University; 2012. [cited 2021 Mar 01]. Available from: http://hdl.handle.net/10161/5601.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hunter G. The Role of Mechanically Gated Ion Channels in Dorsal Closure During Drosophila Morphogenesis . [Thesis]. Duke University; 2012. Available from: http://hdl.handle.net/10161/5601

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

21. Bailles, Anaïs. Intercellular coupling and mechanical feedback during tissue morphogenesis : Couplages intercellulaires et rétrocontrôles mécaniques au cours de la morphogenèse.

Degree: Docteur es, Biologie du developpement, 2018, Aix Marseille Université

URL: http://www.theses.fr/2018AIXM0499

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Un des mécanismes principaux de la morphogenèse des organismes est la contraction des réseaux d’actine sous l’effet du moteur moléculaire Myosine II. L’invagination de l’endoderme… (more)

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Un des mécanismes principaux de la morphogenèse des organismes est la contraction des réseaux d’actine sous l’effet du moteur moléculaire Myosine II. L’invagination de l’endoderme postérieur de Drosophila est causée par la contraction apicale des cellules par MyoII, mais la cause de sa déformation polarisée est inconnue. Nous avons découvert une vague de Rho1, MyoII et de déformation qui se propage à l’échelle du tissu et sous-tend la déformation de l’endoderme. MyoII est d’abord activée dans le primordium de l’endoderme par un ligand de GPCR, Fog. L’activation apicale de MyoII se propage ensuite à travers l’épithélium dorsal à 2.2 ± 0.2 µm/min. La dynamique de la vague n’est définie ni par les niveaux de Fog ni par leur motif d’expression. A la place, l’activité de MyoII est nécessaire pour l’activation intracellulaire de Rho1 et sa propagation à travers le tissu, indiquant une boucle de rétroaction. Des simulations d’un matériau viscoélastique contractile montrent qu’une boucle de rétroaction basée sur la tension peut générer une vague. Des perturbations de l’environnement mécanique du tissu avec des moyens génétiques ou mécaniques résultent en une augmentation de l’activité de MyoII et une diminution de la vitesse de la vague. Les déformations ou les forces du tissu procurent donc un rétrocontrôle sur l’activation de Rho1/MyoII lors de la vague, contrôlant sa dynamique. A l’échelle cellulaire, la vague de déformation implique la compression basale des cellules et l’étalement et l’adhésion du cortex apical sur la membrane vitelline, suivi d’un détachement. Ainsi la morphogenèse observée émerge de la propagation stimulée mécaniquement d’une vague de déformation 3D.

One of the main mechanisms of organism morphogenesis is the contraction of actin filament networks powered by non-muscle Myosin II motor proteins (MyoII). Drosophila presumptive posterior endoderm invagination is caused by MyoII-dependent apical constriction, but the cause of its polarized deformation is unknown. We unravelled a tissue scale wave of high Rho1 and MyoII activation and deformation which underlies the polarized deformation of the endoderm. MyoII is first activated medio-apically in cells within the endoderm primordium by the GPCR ligand Fog. Subsequently, apical MyoII activation propagates across the dorsal epithelium at a constant speed of 2.2 ± 0.2 µm/min. MyoII wave dynamics are set neither by Fog levels nor expression pattern. Instead, both intracellular Rho1 activation and its propagation across the tissue require sustained MyoII activity, indicating a positive feedback from contractility into Rho1 activity. Through simulations of a contractile viscoelastic material we found that a stress-based feedback loop could generate a wave. Perturbations of the tissue mechanical environment with both genetic and physical means result in an increase in MyoII activity and a strong reduction of the wave speed. Tissue deformation or stress thus provides a feedback onto Rho1/MyoII activity during the wave, controlling its dynamics. At the cell…

Advisors/Committee Members: Lecuit, Thomas (thesis director).

Subjects/Keywords: Morphogenèse; Drosophila; Mécanique; Microscopie; Actomyosine; Actomyosin; Morphogenesis; Drosophila; Mechanics; Time-Lapse microscopy; 571

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bailles, A. (2018). Intercellular coupling and mechanical feedback during tissue morphogenesis : Couplages intercellulaires et rétrocontrôles mécaniques au cours de la morphogenèse. (Doctoral Dissertation). Aix Marseille Université. Retrieved from http://www.theses.fr/2018AIXM0499

Chicago Manual of Style (16^th Edition):

Bailles, Anaïs. “Intercellular coupling and mechanical feedback during tissue morphogenesis : Couplages intercellulaires et rétrocontrôles mécaniques au cours de la morphogenèse.” 2018. Doctoral Dissertation, Aix Marseille Université. Accessed March 01, 2021. http://www.theses.fr/2018AIXM0499.

MLA Handbook (7^th Edition):

Bailles, Anaïs. “Intercellular coupling and mechanical feedback during tissue morphogenesis : Couplages intercellulaires et rétrocontrôles mécaniques au cours de la morphogenèse.” 2018. Web. 01 Mar 2021.

Vancouver:

Bailles A. Intercellular coupling and mechanical feedback during tissue morphogenesis : Couplages intercellulaires et rétrocontrôles mécaniques au cours de la morphogenèse. [Internet] [Doctoral dissertation]. Aix Marseille Université 2018. [cited 2021 Mar 01]. Available from: http://www.theses.fr/2018AIXM0499.

Council of Science Editors:

Bailles A. Intercellular coupling and mechanical feedback during tissue morphogenesis : Couplages intercellulaires et rétrocontrôles mécaniques au cours de la morphogenèse. [Doctoral Dissertation]. Aix Marseille Université 2018. Available from: http://www.theses.fr/2018AIXM0499

University of Helsinki

22. Özge Nilay, Yurdakul. The rigor bond’s interaction with protein denaturation : effects on water-holding of myofibrils.

Degree: Department of Food and Environmental Sciences; Helsingfors universitet, Agrikultur- och forstvetenskapliga fakulteten, Institutionen för livsmedels- och miljövetenskaper, 2016, University of Helsinki

URL: http://hdl.handle.net/10138/172491

► The aim of the experimental work was to understand the rigor bonds’ effect together with protein denaturation on water-holding capacity by breaking the actomyosin bonds… (more)

Subjects/Keywords: PSE; water-holding capacity; protein denaturation; pyrophosphate; actomyosin bonds; Livsmedelsteknologi; Food Technology; Elintarviketeknologia

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APA (6^th Edition):

Özge Nilay, Y. (2016). The rigor bond’s interaction with protein denaturation : effects on water-holding of myofibrils. (Masters Thesis). University of Helsinki. Retrieved from http://hdl.handle.net/10138/172491

Chicago Manual of Style (16^th Edition):

Özge Nilay, Yurdakul. “The rigor bond’s interaction with protein denaturation : effects on water-holding of myofibrils.” 2016. Masters Thesis, University of Helsinki. Accessed March 01, 2021. http://hdl.handle.net/10138/172491.

MLA Handbook (7^th Edition):

Özge Nilay, Yurdakul. “The rigor bond’s interaction with protein denaturation : effects on water-holding of myofibrils.” 2016. Web. 01 Mar 2021.

Vancouver:

Özge Nilay Y. The rigor bond’s interaction with protein denaturation : effects on water-holding of myofibrils. [Internet] [Masters thesis]. University of Helsinki; 2016. [cited 2021 Mar 01]. Available from: http://hdl.handle.net/10138/172491.

Council of Science Editors:

Özge Nilay Y. The rigor bond’s interaction with protein denaturation : effects on water-holding of myofibrils. [Masters Thesis]. University of Helsinki; 2016. Available from: http://hdl.handle.net/10138/172491

Vanderbilt University

23. Carrington, Leolene Jean. The Rho family GEF Asef2 regulates cancer cell migration by modulating Rac activation and actomyosin contractility.

Degree: PhD, Biological Sciences, 2016, Vanderbilt University

URL: http://hdl.handle.net/1803/11651

► Non-muscle myosin II (MyoII) contractility is important to the regulation of many cellular processes, including migration. The small GTPase Rho has been shown to regulate… (more)

Subjects/Keywords: GTPase; adhesion turnover; Myosin II; actomyosin; Rho GEF; TIRF; FRET; extracellular matrix; Asef2; PAK

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Carrington, L. J. (2016). The Rho family GEF Asef2 regulates cancer cell migration by modulating Rac activation and actomyosin contractility. (Doctoral Dissertation). Vanderbilt University. Retrieved from http://hdl.handle.net/1803/11651

Chicago Manual of Style (16^th Edition):

Carrington, Leolene Jean. “The Rho family GEF Asef2 regulates cancer cell migration by modulating Rac activation and actomyosin contractility.” 2016. Doctoral Dissertation, Vanderbilt University. Accessed March 01, 2021. http://hdl.handle.net/1803/11651.

MLA Handbook (7^th Edition):

Carrington, Leolene Jean. “The Rho family GEF Asef2 regulates cancer cell migration by modulating Rac activation and actomyosin contractility.” 2016. Web. 01 Mar 2021.

Vancouver:

Carrington LJ. The Rho family GEF Asef2 regulates cancer cell migration by modulating Rac activation and actomyosin contractility. [Internet] [Doctoral dissertation]. Vanderbilt University; 2016. [cited 2021 Mar 01]. Available from: http://hdl.handle.net/1803/11651.

Council of Science Editors:

Carrington LJ. The Rho family GEF Asef2 regulates cancer cell migration by modulating Rac activation and actomyosin contractility. [Doctoral Dissertation]. Vanderbilt University; 2016. Available from: http://hdl.handle.net/1803/11651

University of New South Wales

24. Lyttleton, Roman. Development of solid-state nanodevices for studying proteins.

Degree: Physics, 2018, University of New South Wales

URL: http://handle.unsw.edu.au/1959.4/60335 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:51866/SOURCE02?view=true

► In both lab-on-a-chip applications and biocomputation, being able to easily track a molecule passing a particular point along a channel is heavily desirable. This thesis… (more)

▼ In both lab-on-a-chip applications and biocomputation, being able to easily track a molecule passing a particular point along a channel is heavily desirable. This thesis has centred on electrical detection of passing filaments in an actomyosin in vitro motility assay (IVMA). This would enable tracking large numbers of agents at once, an important advance in scaling up parallel processing biocomputers. We built a custom CVD furnace for growing CNTs that safely handled a variety of input gases with precise flow rate and temperature control. Ethanol vapour was found to be a superior feedstock gas to methane, and EBL-defined Fe islands more reliably catalysed CNTs of the appropriate density than solution deposited FeMoC or alumina nanoparticles. The definition of Fe islands was improved by the design and optimisation of a photolithography process that allowed batch-fabrication of many samples in parallel. CNFET contacts made from Cr, Ti, and Pd were compared and Pd was found to be the most reliable for high quality CNFETs that lasted more than 3 weeks with little degradation.The best performing devices had on-state conductances of up to 2 µS and subthreshold swings as low as 0.3 V/dec for liquid gated measurements although leakage currents through our EBL resist encapsulation between the liquid gate and the source-drain contacts were present. These leakage currents could be removed by ensuring a particle-free surface or doubling the thickness of the encapsulating resist. A Faraday cage was integrated with a fluorescence microscope to assist with electrical detection of a passing actin filament by simultaneous electrical and optical measurement. We developed a method to raise CNFETs up to the actin travelling height and combine them with the required nanostructures for actomyosin motility on the same substrate. We demonstrate the potential for a thin layer of parylene to coat the CNFET, preventing myosin binding without adversely affecting electrical detection or filament crossing. A novel EBL process was developed to fabricate thin strips of Nafion with resolution around 1 µm This process was used to create Nafion wrap-gate InAs transistor, an ionic transducer, which was characterised in several measurement ranges and atmospheric conditions. Advisors/Committee Members: Micolich, Adam, Physics, Faculty of Science, UNSW, Curmi, Paul, Physics, Faculty of Science, UNSW.

Subjects/Keywords: Carbon nanotube field effect transistors; Biocomputation; Carbon nanotubes; Biosensing; Nafion; Actomyosin; Actin; Myosin

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lyttleton, R. (2018). Development of solid-state nanodevices for studying proteins. (Doctoral Dissertation). University of New South Wales. Retrieved from http://handle.unsw.edu.au/1959.4/60335 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:51866/SOURCE02?view=true

Chicago Manual of Style (16^th Edition):

Lyttleton, Roman. “Development of solid-state nanodevices for studying proteins.” 2018. Doctoral Dissertation, University of New South Wales. Accessed March 01, 2021. http://handle.unsw.edu.au/1959.4/60335 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:51866/SOURCE02?view=true.

MLA Handbook (7^th Edition):

Lyttleton, Roman. “Development of solid-state nanodevices for studying proteins.” 2018. Web. 01 Mar 2021.

Vancouver:

Lyttleton R. Development of solid-state nanodevices for studying proteins. [Internet] [Doctoral dissertation]. University of New South Wales; 2018. [cited 2021 Mar 01]. Available from: http://handle.unsw.edu.au/1959.4/60335 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:51866/SOURCE02?view=true.

Council of Science Editors:

Lyttleton R. Development of solid-state nanodevices for studying proteins. [Doctoral Dissertation]. University of New South Wales; 2018. Available from: http://handle.unsw.edu.au/1959.4/60335 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:51866/SOURCE02?view=true

25. Munjal, Akankshi. Regulation of a bio-mechanical network driving shape changes during tissue morphogenesis : Régulation d'un réseau biomécanique entraînant des changements de forme lors de morphogenese des tissus.

Degree: Docteur es, Biologie du developpement, 2015, Aix Marseille Université

URL: http://www.theses.fr/2015AIXM4038

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Forces requises pour les changements de forme au cours de la morphogenèse des tissus sont générés par d’actine et de myosine. Durant ma thèse, je… (more)

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Forces requises pour les changements de forme au cours de la morphogenèse des tissus sont générés par d’actine et de myosine. Durant ma thèse, je étudié le rôle de la réglementation MyoII par la voie Rho1-Rok durant l’élongation de l’ectoderme ventro-latéral par intercalation cellulaire. Les pulsations de MyoII médio-apicale se déplacent de manière anisotrope vers les jonctions parallèles avec l’axe dorso-ventral (ou jonctions verticales). Ceci provoque le rétrécissement graduel des jonctions qui sont stabilisées par une population de MyoII polarisée dans le plan du tissu et enrichie au niveau de ces jonctions. Les mécanismes cellulaires qui régulent la pulsatilité, la stabilité et la polarité de la myosine II restent à élucider. J’ai identifié deux propriétés cruciales de la dynamique de la myosine II régie par phospho- à savoir la cinétique d’échange gouvernée par les cycles de phosphorylation-déphosphorylation des chaines légères régulatrices de la MyoII (RLC) et l’advection due à la contraction des moteurs sur le réseau de F-actine. Contrôle spatial sur le chiffre d'affaires MyoII établit 2 régimes stables des taux élevés et faibles dissociation résultant dans MyoII polarité. Pulsatilité est un comportement auto-organisé qui émerge à taux de dissociation intermédiaires permettant d'advection MyoII et les régulateurs en amont. Dans la deuxième partie de ma thèse, je l'ai montré que la protéine GPCR- GRsmog et la brume, et la voie G-protéines en aval permettent l'activation progressive des MyoII, établissant pulsatilité et de la stabilité pour produire des déformations de forme polarisées cours de la morphogenèse.

Forces required to power shape changes during tissue morphogenesis are generated by non-muscle MyosinII (MyoII) pulling filamentous actin. During my PhD, I investigated the role of MyoII regulation through the conserved Rho1-Rok pathway during Drosophila germband extension. The morphogenetic process is powered by cell intercalation involving shrinkage of junctions in the dorsal-ventral axis (‘vertical junctions’) followed by junction extension in the anterior-posterior axis. Advances in light microscopy revealed that the actomyosin networks exhibit pulsed contractions to power junction shrinkage, and alternate with steps of stabilization by MyoII enriched on vertical junctions (planar-polarity) to result in irreversible shape changes. Although described in many different contexts, the underlying mechanisms of this ratchet-like behavior remained unclear. Using genetic and biophysical tools, quantitative imaging and subtle perturbations, I identified 2 critical properties underlying MyoII dynamics- turnover governed by phospho-cycling of the MyoII Regulatory Light Chain, and advection due to contraction of the motors on actin networks. Spatial control over MyoII turnover establishes 2 stable regimes of high and low dissociation rates resulting in MyoII planar polarity. Pulsatility is a self-organized behavior that emerges at intermediate dissociation rates enabling advection of MyoII and upstream…

Advisors/Committee Members: Lecuit, Thomas (thesis director).

Subjects/Keywords: Morphogenèse du tissu; Polarité; Gastrulation; Drosophile; Les réseaux de actomyosine; Gpcr; Tissue morphogenesis; Polarity; Gastrulation; Drosophila; Actomyosin networks; Gpcr; 570

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APA (6^th Edition):

Munjal, A. (2015). Regulation of a bio-mechanical network driving shape changes during tissue morphogenesis : Régulation d'un réseau biomécanique entraînant des changements de forme lors de morphogenese des tissus. (Doctoral Dissertation). Aix Marseille Université. Retrieved from http://www.theses.fr/2015AIXM4038

Chicago Manual of Style (16^th Edition):

Munjal, Akankshi. “Regulation of a bio-mechanical network driving shape changes during tissue morphogenesis : Régulation d'un réseau biomécanique entraînant des changements de forme lors de morphogenese des tissus.” 2015. Doctoral Dissertation, Aix Marseille Université. Accessed March 01, 2021. http://www.theses.fr/2015AIXM4038.

MLA Handbook (7^th Edition):

Munjal, Akankshi. “Regulation of a bio-mechanical network driving shape changes during tissue morphogenesis : Régulation d'un réseau biomécanique entraînant des changements de forme lors de morphogenese des tissus.” 2015. Web. 01 Mar 2021.

Vancouver:

Munjal A. Regulation of a bio-mechanical network driving shape changes during tissue morphogenesis : Régulation d'un réseau biomécanique entraînant des changements de forme lors de morphogenese des tissus. [Internet] [Doctoral dissertation]. Aix Marseille Université 2015. [cited 2021 Mar 01]. Available from: http://www.theses.fr/2015AIXM4038.

Council of Science Editors:

Munjal A. Regulation of a bio-mechanical network driving shape changes during tissue morphogenesis : Régulation d'un réseau biomécanique entraînant des changements de forme lors de morphogenese des tissus. [Doctoral Dissertation]. Aix Marseille Université 2015. Available from: http://www.theses.fr/2015AIXM4038

26. Rothschild, Pierre-Raphaël. Remaniements du cytosquelette des barrières hémato-rétiniennes au cours de la rétinopathie diabétique : implications physiopathologiques et thérapeutiques : rôle de la PKCζ et de la voie Rho/ROCK/Myosine II : ROCK controls blood-retinal barrier breakdown and capillary perfusion in diabetic retinopathy : therapeutic implication.

Degree: Docteur es, Neurosciences, 2015, Sorbonne Paris Cité

URL: http://www.theses.fr/2015USPCB130

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La rétinopathie diabétique (RD) se compose d’une part d’une ischémie rétinienne périphérique et d’autre part d’une exsudation rétinienne responsable d’un œdème maculaire diabétique, première cause… (more)

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La rétinopathie diabétique (RD) se compose d’une part d’une ischémie rétinienne périphérique et d’autre part d’une exsudation rétinienne responsable d’un œdème maculaire diabétique, première cause de cécité chez les moins 55 ans. Les traitements utilisés actuellement sont non spécifiques et traitent les complications tardives de la RD. Les phases précoces de la RD ne sont donc pas ciblées. L’hyperglycémie chronique entraine un stress oxydant et une activation des PKC qui participent à l’altération des BHR. L’objectif de ce travail a été 1°) d’étudier l’implication de la PKCζ et de la voie Rho/ROCK/Myosine II sur la physiopathogénie de la RD et 2°) de montrer l’effet bénéfique de leurs inhibiteur sur les BHR et sur la reperfusion des capillaires rétiniens. Nous avons confirmé l’hyperactivation de la PKCζ et de la voie Rho/ROCK/Myosine II chez les rats diabétiques et leur participation à la rupture de la BHR externe. Le traitement par leurs inhibiteurs respectifs normalise l’activation des deux enzymes et restaure l’intégrité anatomique et fonctionnelle de la BHR externe. De plus l'hyperactivation de ROCK altère la perfusion rétinienne par 1) constriction focale artériolaire, 2) protrusions membranaires endoluminales des cellules endothéliales (blebbing) et 3) vasoconstriction capillaire diffuse. Nous avons montré que l'ensemble de ces phénomènes étaient réversibles par traitement intravitréen de son inhibiteur le Fasudil. De manière importante le traitement par Fasudil induit également une diminution du VEGF rétinien responsable de la perméabilité des barrières et témoin indirect de l’ischémie rétinienne. Ces travaux éclairent la physiopathogénie de la RD et ouvre des perspectives thérapeutiques permettant de cibler les événements précoces de la RD.

Diabetic retinopathy (DR) mainly results from peripheral retinal ischemia and exudation leading to sight threatening complications such as retinal neovascularization or macular edema. This latter represents the main cause of visual loss among working age individuals. Current treatments address late complications of DR and are non-specific. Therefore, early events are currently not addressed. Chronic hyperglycemia increases oxidative stress and activates PKC leading to blood retinal barrier (BRB) breakdown. The aims of the present work were two fold. First, to assess the implication of PKCζ and the Rho/ROCK/Myosin II pathway on the pathogenesis of DR and second, to assess whether their specific inhibitors have the potential to restore the phenotype. Herein we have demonstrated the pathogenic role of PCKζ and ROCK hyperactivation on the development of diabetes induced external BRB breakdown. Furthermore their inhibitors restored the morphologic and functional aspect of the external BRB. We also found that ROCK hyperactivation was responsible for altered retinal perfusion through several mechanism namely 1) focal constriction of retinal arterioles; 2) endoluminal protrusions of the endothelial cell membrane (blebs) and 3) capillary diffuse vasoconstriction. We were able to…

Advisors/Committee Members: Crisanti-Lassiaz, Patricia (thesis director).

Subjects/Keywords: Rétinopathie diabétique; Diabetic retinopathy; Macular oedema; ROCK1; Fasudil; Blood retinal barrier; Diabetes; Goto Kakizaki; Actomyosin; Blebs; 573.8

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Rothschild, P. (2015). Remaniements du cytosquelette des barrières hémato-rétiniennes au cours de la rétinopathie diabétique : implications physiopathologiques et thérapeutiques : rôle de la PKCζ et de la voie Rho/ROCK/Myosine II : ROCK controls blood-retinal barrier breakdown and capillary perfusion in diabetic retinopathy : therapeutic implication. (Doctoral Dissertation). Sorbonne Paris Cité. Retrieved from http://www.theses.fr/2015USPCB130

Chicago Manual of Style (16^th Edition):

Rothschild, Pierre-Raphaël. “Remaniements du cytosquelette des barrières hémato-rétiniennes au cours de la rétinopathie diabétique : implications physiopathologiques et thérapeutiques : rôle de la PKCζ et de la voie Rho/ROCK/Myosine II : ROCK controls blood-retinal barrier breakdown and capillary perfusion in diabetic retinopathy : therapeutic implication.” 2015. Doctoral Dissertation, Sorbonne Paris Cité. Accessed March 01, 2021. http://www.theses.fr/2015USPCB130.

MLA Handbook (7^th Edition):

Rothschild, Pierre-Raphaël. “Remaniements du cytosquelette des barrières hémato-rétiniennes au cours de la rétinopathie diabétique : implications physiopathologiques et thérapeutiques : rôle de la PKCζ et de la voie Rho/ROCK/Myosine II : ROCK controls blood-retinal barrier breakdown and capillary perfusion in diabetic retinopathy : therapeutic implication.” 2015. Web. 01 Mar 2021.

Vancouver:

Rothschild P. Remaniements du cytosquelette des barrières hémato-rétiniennes au cours de la rétinopathie diabétique : implications physiopathologiques et thérapeutiques : rôle de la PKCζ et de la voie Rho/ROCK/Myosine II : ROCK controls blood-retinal barrier breakdown and capillary perfusion in diabetic retinopathy : therapeutic implication. [Internet] [Doctoral dissertation]. Sorbonne Paris Cité; 2015. [cited 2021 Mar 01]. Available from: http://www.theses.fr/2015USPCB130.

Council of Science Editors:

Rothschild P. Remaniements du cytosquelette des barrières hémato-rétiniennes au cours de la rétinopathie diabétique : implications physiopathologiques et thérapeutiques : rôle de la PKCζ et de la voie Rho/ROCK/Myosine II : ROCK controls blood-retinal barrier breakdown and capillary perfusion in diabetic retinopathy : therapeutic implication. [Doctoral Dissertation]. Sorbonne Paris Cité; 2015. Available from: http://www.theses.fr/2015USPCB130

Universitat Pompeu Fabra

27. Sumi, Angughali Aheto, 1986-. On contractile actomyosin waves and their role in junctional remodeling during epithelial constriction.

Degree: Departament de Ciències Experimentals i de la Salut, 2017, Universitat Pompeu Fabra

URL: http://hdl.handle.net/10803/565600

► Los tejidos epiteliales llevan a cabo una remodelación extensiva durante el desarrollo embrionario. Estudios recientes han revelada que, en un sinnumero de procesos de desarrollo… (more)

▼ Los tejidos epiteliales llevan a cabo una remodelación extensiva durante el desarrollo embrionario. Estudios recientes han revelada que, en un sinnumero de procesos de desarrollo embrionario, la remodelación epitelial se asocia con pulsaciones de áreas en células individuales y con flujos corticales de actomiosina. Durante el cierre dorsal de Drosophila, la amnioserosa (AS), un tejido contractil que cubre la región dorsal del embrión, se observan pulsaciones contráctiles en células individuales y flujos regulares de actomiosina durante la reducción de la superficial apical celular. Al día de hoy, no se conoce el mecanismo biofísico que produce estas pulsaciones celulares ni y el papel que tienen las oscilaciones contráctiles de actomiosina en el epitelio del cierre dorsal embrionario. En este proyecto, se desarrolló un modelo biofísico para entender estas oscilaciones celulares. El modelo se basa en propiedades intrínsecas de la célula como la rotación de la corteza celular, la contractilidad activa mediante moléculas productoras de fuerza y la elasticidad celular. Utilizando éste modelo, se muestra que acoplando estas tres propiedades clave es suficiente para generar oscilaciones celulares estables. Además, dentro de este marco, se han generado oscilaciones mediante el acoplamiento de varias unidades oscilantes y la introducción de un término de difusión para considerar el intercambio de moléculas productoras de fuerza entre las unidades. A continuación, se investigó el papel de estas oscilaciones contráctiles de actomiosina en la remodelación de tejidos. Como resultado, se desarrolla una técnica innovadora que permite aplicar extensión mecánica al tejido de AS y estudiar la respuesta celular ante tal estrés. Con este método, se pueden detener las pulsaciones contráctiles y los flujos de actomiosina en células de la AS. Se muestra que este arresto celular está asociado con la relocalización de actina y miosina de la región central de las células hacia las uniones adherentes intercelulares para mantener su integridad durante la extension epitelial. Esta relocalización de miosina se correlaciona directamente con la tensión en uniones intercelulares y no ocurre en células en las que el reciclaje cellular a través de endocitosis se ha bloqueado. El resultado es un exceso en la acumulación de membrana plasmática en células oscilantes que no responden a la extension epitelial. Tras liberar al tejido de la extension epithelial, la miosina se relocaliza a la área central de las células y las pulsaciones continuan. Esto indica que las células pueden cambiar entre dos estados según la tension aplicada: uno dónde las células muestran oscilaciones asociadas con pulsaciones contráctiles de actomiosina, y otra donde la forma celular se establece con la localización preferente de miosina en las uniones intercelulares. Además, tras liberar el tejido de una extensión de alta duración (>10mins), las uniones intercelulares sufrieron corrugaciones. La localización consistente de oscilaciones de miosina en las regions corrugadas,… Advisors/Committee Members: [email protected] (authoremail), true (authoremailshow), Solon, Jérôme (director), true (authorsendemail).

Subjects/Keywords: Actomyosin; Endocytosis; Actomiosina; Endocitosis; Contractile pulses; Mechanical stress; Adherens junction; Pulsaciones contráctiles; Estrés mecánica; Uniones adherentes; Adherens junction; 577

Record Details Similar Records Cite « Share

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sumi, Angughali Aheto, 1. (2017). On contractile actomyosin waves and their role in junctional remodeling during epithelial constriction. (Thesis). Universitat Pompeu Fabra. Retrieved from http://hdl.handle.net/10803/565600

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Sumi, Angughali Aheto, 1986-. “On contractile actomyosin waves and their role in junctional remodeling during epithelial constriction.” 2017. Thesis, Universitat Pompeu Fabra. Accessed March 01, 2021. http://hdl.handle.net/10803/565600.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Sumi, Angughali Aheto, 1986-. “On contractile actomyosin waves and their role in junctional remodeling during epithelial constriction.” 2017. Web. 01 Mar 2021.

Vancouver:

Sumi, Angughali Aheto 1. On contractile actomyosin waves and their role in junctional remodeling during epithelial constriction. [Internet] [Thesis]. Universitat Pompeu Fabra; 2017. [cited 2021 Mar 01]. Available from: http://hdl.handle.net/10803/565600.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Sumi, Angughali Aheto 1. On contractile actomyosin waves and their role in junctional remodeling during epithelial constriction. [Thesis]. Universitat Pompeu Fabra; 2017. Available from: http://hdl.handle.net/10803/565600

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universitat Pompeu Fabra

28. Johnsen, Lisa, 1987-. Lipid droplet regulation by the differentially spliced proteins Osw5L and Osw5S.

Degree: Departament de Ciències Experimentals i de la Salut, 2016, Universitat Pompeu Fabra

URL: http://hdl.handle.net/10803/565566

► Los tejidos epiteliales llevan a cabo una remodelación extensiva durante el desarrollo embrionario. Estudios recientes han revelada que, en un sinnumero de procesos de desarrollo… (more)

▼ Los tejidos epiteliales llevan a cabo una remodelación extensiva durante el desarrollo embrionario. Estudios recientes han revelada que, en un sinnumero de procesos de desarrollo embrionario, la remodelación epitelial se asocia con pulsaciones de áreas en células individuales y con flujos corticales de actomiosina. Durante el cierre dorsal de Drosophila, la amnioserosa (AS), un tejido contractil que cubre la región dorsal del embrión, se observan pulsaciones contráctiles en células individuales y flujos regulares de actomiosina durante la reducción de la superficial apical celular. Al día de hoy, no se conoce el mecanismo biofísico que produce estas pulsaciones celulares ni y el papel que tienen las oscilaciones contráctiles de actomiosina en el epitelio del cierre dorsal embrionario. En este proyecto, se desarrolló un modelo biofísico para entender estas oscilaciones celulares. El modelo se basa en propiedades intrínsecas de la célula como la rotación de la corteza celular, la contractilidad activa mediante moléculas productoras de fuerza y la elasticidad celular. Utilizando éste modelo, se muestra que acoplando estas tres propiedades clave es suficiente para generar oscilaciones celulares estables. Además, dentro de este marco, se han generado oscilaciones mediante el acoplamiento de varias unidades oscilantes y la introducción de un término de difusión para considerar el intercambio de moléculas productoras de fuerza entre las unidades. A continuación, se investigó el papel de estas oscilaciones contráctiles de actomiosina en la remodelación de tejidos. Como resultado, se desarrolla una técnica innovadora que permite aplicar extensión mecánica al tejido de AS y estudiar la respuesta celular ante tal estrés. Con este método, se pueden detener las pulsaciones contráctiles y los flujos de actomiosina en células de la AS. Se muestra que este arresto celular está asociado con la relocalización de actina y miosina de la región central de las células hacia las uniones adherentes intercelulares para mantener su integridad durante la extension epitelial. Esta relocalización de miosina se correlaciona directamente con la tensión en uniones intercelulares y no ocurre en células en las que el reciclaje cellular a través de endocitosis se ha bloqueado. El resultado es un exceso en la acumulación de membrana plasmática en células oscilantes que no responden a la extension epitelial. Tras liberar al tejido de la extension epithelial, la miosina se relocaliza a la área central de las células y las pulsaciones continuan. Esto indica que las células pueden cambiar entre dos estados según la tension aplicada: uno dónde las células muestran oscilaciones asociadas con pulsaciones contráctiles de actomiosina, y otra donde la forma celular se establece con la localización preferente de miosina en las uniones intercelulares. Además, tras liberar el tejido de una extensión de alta duración (>10mins), las uniones intercelulares sufrieron corrugaciones. La localización consistente de oscilaciones de miosina en las regions corrugadas,… Advisors/Committee Members: [email protected] (authoremail), true (authoremailshow), Carvalho, Pedro (director), true (authorsendemail).

Subjects/Keywords: Actomyosin; Contractile pulses; Mechanical stress; Adherens junction; Endocytosis; Actomiosina; Pulsaciones contráctiles; Estrés mecánica; Uniones adherentes; Endocitosis; 577

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Johnsen, Lisa, 1. (2016). Lipid droplet regulation by the differentially spliced proteins Osw5L and Osw5S. (Thesis). Universitat Pompeu Fabra. Retrieved from http://hdl.handle.net/10803/565566

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Johnsen, Lisa, 1987-. “Lipid droplet regulation by the differentially spliced proteins Osw5L and Osw5S.” 2016. Thesis, Universitat Pompeu Fabra. Accessed March 01, 2021. http://hdl.handle.net/10803/565566.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Johnsen, Lisa, 1987-. “Lipid droplet regulation by the differentially spliced proteins Osw5L and Osw5S.” 2016. Web. 01 Mar 2021.

Vancouver:

Johnsen, Lisa 1. Lipid droplet regulation by the differentially spliced proteins Osw5L and Osw5S. [Internet] [Thesis]. Universitat Pompeu Fabra; 2016. [cited 2021 Mar 01]. Available from: http://hdl.handle.net/10803/565566.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Johnsen, Lisa 1. Lipid droplet regulation by the differentially spliced proteins Osw5L and Osw5S. [Thesis]. Universitat Pompeu Fabra; 2016. Available from: http://hdl.handle.net/10803/565566

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

29. TAN SONG HUI. TEMPORAL ASSOCIATION OF TENSIN1 AND P130CAS AT FOCAL ADHESIONS LINKS ACTOMYOSIN CONTRACTION TO CELL MIGRATION.

Degree: 2014, National University of Singapore

URL: http://scholarbank.nus.edu.sg/handle/10635/118657

Subjects/Keywords: Tensin1; p130Cas; focal adhesions; actomyosin contraction; cell migration

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

HUI, T. S. (2014). TEMPORAL ASSOCIATION OF TENSIN1 AND P130CAS AT FOCAL ADHESIONS LINKS ACTOMYOSIN CONTRACTION TO CELL MIGRATION. (Thesis). National University of Singapore. Retrieved from http://scholarbank.nus.edu.sg/handle/10635/118657

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

HUI, TAN SONG. “TEMPORAL ASSOCIATION OF TENSIN1 AND P130CAS AT FOCAL ADHESIONS LINKS ACTOMYOSIN CONTRACTION TO CELL MIGRATION.” 2014. Thesis, National University of Singapore. Accessed March 01, 2021. http://scholarbank.nus.edu.sg/handle/10635/118657.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

HUI, TAN SONG. “TEMPORAL ASSOCIATION OF TENSIN1 AND P130CAS AT FOCAL ADHESIONS LINKS ACTOMYOSIN CONTRACTION TO CELL MIGRATION.” 2014. Web. 01 Mar 2021.

Vancouver:

HUI TS. TEMPORAL ASSOCIATION OF TENSIN1 AND P130CAS AT FOCAL ADHESIONS LINKS ACTOMYOSIN CONTRACTION TO CELL MIGRATION. [Internet] [Thesis]. National University of Singapore; 2014. [cited 2021 Mar 01]. Available from: http://scholarbank.nus.edu.sg/handle/10635/118657.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

HUI TS. TEMPORAL ASSOCIATION OF TENSIN1 AND P130CAS AT FOCAL ADHESIONS LINKS ACTOMYOSIN CONTRACTION TO CELL MIGRATION. [Thesis]. National University of Singapore; 2014. Available from: http://scholarbank.nus.edu.sg/handle/10635/118657

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

30. EKTA MAKHIJA. CELL GEOMETRIC CONSTRAINTS REGULATE NUCLEAR AND CHROMATIN PLASTICITY VIA ACTOMYOSIN CONTRACTILITY.

Degree: 2015, National University of Singapore

URL: http://scholarbank.nus.edu.sg/handle/10635/121985

Subjects/Keywords: Mechanotransduction; Cell Geometry; Actomyosin Contractility; Nuclear Plasticity; Chromatin Dynamics

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

MAKHIJA, E. (2015). CELL GEOMETRIC CONSTRAINTS REGULATE NUCLEAR AND CHROMATIN PLASTICITY VIA ACTOMYOSIN CONTRACTILITY. (Thesis). National University of Singapore. Retrieved from http://scholarbank.nus.edu.sg/handle/10635/121985

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

MAKHIJA, EKTA. “CELL GEOMETRIC CONSTRAINTS REGULATE NUCLEAR AND CHROMATIN PLASTICITY VIA ACTOMYOSIN CONTRACTILITY.” 2015. Thesis, National University of Singapore. Accessed March 01, 2021. http://scholarbank.nus.edu.sg/handle/10635/121985.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

MAKHIJA, EKTA. “CELL GEOMETRIC CONSTRAINTS REGULATE NUCLEAR AND CHROMATIN PLASTICITY VIA ACTOMYOSIN CONTRACTILITY.” 2015. Web. 01 Mar 2021.

Vancouver:

MAKHIJA E. CELL GEOMETRIC CONSTRAINTS REGULATE NUCLEAR AND CHROMATIN PLASTICITY VIA ACTOMYOSIN CONTRACTILITY. [Internet] [Thesis]. National University of Singapore; 2015. [cited 2021 Mar 01]. Available from: http://scholarbank.nus.edu.sg/handle/10635/121985.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

MAKHIJA E. CELL GEOMETRIC CONSTRAINTS REGULATE NUCLEAR AND CHROMATIN PLASTICITY VIA ACTOMYOSIN CONTRACTILITY. [Thesis]. National University of Singapore; 2015. Available from: http://scholarbank.nus.edu.sg/handle/10635/121985

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Open Access Theses and Dissertations