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You searched for subject:(actin myosin interaction). Showing records 1 – 2 of 2 total matches.

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University of Guelph

1. Mishra, Vadika. Characterization of short ADP ribosylated oligomers developed by modification in F-actin structure.

Degree: MS, Department of Molecular and Cellular Biology, 2015, University of Guelph

Researchers have been trying to determine the mechanism of the crossbridge cycle, containing actomyosin complex, formed by the interaction of F-actin and myosin. But, because actin forms polymers of varying lengths, it has been difficult to crystallize this complex. My research is aimed at developing a homogeneous actomyosin complex with a short actin oligomer that possesses one myosin-binding site. ADPr-oligomers are produced by chemical crosslinking of F-actin and inhibiting polymerization by ADP-ribosylation. Specifically, I determined the behavior of an ADPr-trimer and dimer with myosin and its effect on actomyosin activity. Individual myosin ATPase activities of ADPr-trimer/dimer were similar to basal myosin activity. However, in the presence of long ADPr-oligomers (tetramer, pentamer, etc.) or F-actin, ADPr-dimer/trimer show binding and activity with myosin thick filaments. Thus, I was unable to produce a short actomyosin complex through these efforts and present a model for interaction of short actin oligomers with each other in the presence of long actin filaments and myosin. Advisors/Committee Members: Dawson, John (advisor).

Subjects/Keywords: actin; myosin; interaction; actomyosin; F-actin

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Mishra, V. (2015). Characterization of short ADP ribosylated oligomers developed by modification in F-actin structure. (Masters Thesis). University of Guelph. Retrieved from https://atrium.lib.uoguelph.ca/xmlui/handle/10214/8714

Chicago Manual of Style (16th Edition):

Mishra, Vadika. “Characterization of short ADP ribosylated oligomers developed by modification in F-actin structure.” 2015. Masters Thesis, University of Guelph. Accessed March 01, 2021. https://atrium.lib.uoguelph.ca/xmlui/handle/10214/8714.

MLA Handbook (7th Edition):

Mishra, Vadika. “Characterization of short ADP ribosylated oligomers developed by modification in F-actin structure.” 2015. Web. 01 Mar 2021.

Vancouver:

Mishra V. Characterization of short ADP ribosylated oligomers developed by modification in F-actin structure. [Internet] [Masters thesis]. University of Guelph; 2015. [cited 2021 Mar 01]. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/8714.

Council of Science Editors:

Mishra V. Characterization of short ADP ribosylated oligomers developed by modification in F-actin structure. [Masters Thesis]. University of Guelph; 2015. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/8714

2. 王, 志丹. Caffeine inhibits smooth muscle actin-myosin interaction : カフェインは平滑筋ミオシンアクチン相互作用を抑制する.

Degree: 修士(健康科学), 2017, Tokyo Metropolitan University / 首都大学東京

Caffeine is known to have inhibitory effects on Ca^<2+>-induced contraction in smooth muscle, and possible mechanisms may contribute the inhibitory effects. Recently, it was found a caffeine effect on direct inhibition of myosin-actin interaction in smooth muscle. To clarify whether direct inhibition of myosin-actin interaction of caffeine to be dependent on the regulation of myosin light chain phosphorylation, in the present study, the author examined caffeine effects on skinned (cell membrane permeabilized) smooth muscle contraction. At 10 mM, caffeine significantly suppressed Ca^<2+> induced contraction, which was dependent on myosin light chain phosphorylation. On the other hand, caffeine did not affect 15.5 mM Mg^<2+> induced contraction, which was independent of myosin light chain phosphorylation. The present results suggest that caffeine inhibits actin-myosin interaction of smooth muscle through either inhibition of myosin light chain kinase activity or acceleration of myosin phosphatase activity.

カフェインには平滑筋にCaイオンの抑制があることが知られている。様々な機構がこの抑制に関与している。最近、カフェインは平滑筋のミオシンアクチン相互作用を直接的に抑制することが明らかになった。本研究では、細胞膜を破壊したスキンド盲腸紐標本を用いて、カフェインの平滑筋アクチン・ミオシン相互作用の抑制が、ミオシン軽鎖リン酸化の抑制によるものなのかを検討した。10mMのカェインはミオシン軽鎖リン酸化に依存するCaイオン活性化収縮張力を抑制した。一方、カフェインはミオシン軽鎖リン酸化に依存しない15.5mM Mgイオンによる収縮張力を抑制しない。以上から、カフェインはミオシン軽鎖リン酸化酵素の活性阻害、もしくはミオシンボスファターゼの活性を促進することでミオシン軽鎖リン酸化レベルを低下させることで、平滑筋ミオシンアクチン相互作用を抑制し、平滑筋収縮を抑制することが示唆された。

首都大学東京, 2017-03-25, 修士(健康科学)

Subjects/Keywords: caffeine; Ca^<; 2+>; -induced contraction; myosin-actin interaction; myosin light chain phosphorylation; カフェイン; Ca^<; 2+>; 活性化収縮; ミオシン-アクチン相互作用; ミオシン軽鎖リン酸化

Record DetailsSimilar RecordsGoogle PlusoneFacebookTwitterCiteULikeMendeleyreddit

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

王, . (2017). Caffeine inhibits smooth muscle actin-myosin interaction : カフェインは平滑筋ミオシンアクチン相互作用を抑制する. (Thesis). Tokyo Metropolitan University / 首都大学東京. Retrieved from http://hdl.handle.net/10748/00009635

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

王, 志丹. “Caffeine inhibits smooth muscle actin-myosin interaction : カフェインは平滑筋ミオシンアクチン相互作用を抑制する.” 2017. Thesis, Tokyo Metropolitan University / 首都大学東京. Accessed March 01, 2021. http://hdl.handle.net/10748/00009635.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

王, 志丹. “Caffeine inhibits smooth muscle actin-myosin interaction : カフェインは平滑筋ミオシンアクチン相互作用を抑制する.” 2017. Web. 01 Mar 2021.

Vancouver:

王 . Caffeine inhibits smooth muscle actin-myosin interaction : カフェインは平滑筋ミオシンアクチン相互作用を抑制する. [Internet] [Thesis]. Tokyo Metropolitan University / 首都大学東京; 2017. [cited 2021 Mar 01]. Available from: http://hdl.handle.net/10748/00009635.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

王 . Caffeine inhibits smooth muscle actin-myosin interaction : カフェインは平滑筋ミオシンアクチン相互作用を抑制する. [Thesis]. Tokyo Metropolitan University / 首都大学東京; 2017. Available from: http://hdl.handle.net/10748/00009635

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

.