subject:(Yeast genetics)

University of Texas – Austin

1. Laurent, Jon Michael. Evolutionary conservation of protein abundance and function.

Degree: PhD, Cellular and Molecular Biology, 2016, University of Texas – Austin

► Conservation lies at the heart of biology. All organisms on earth are descended from a common ancestor, resulting in the preservation of many biological properties,… (more)

▼ Conservation lies at the heart of biology. All organisms on earth are descended from a common ancestor, resulting in the preservation of many biological properties, from genotype to gene function and phenotype. The advent of next generation nucleic acid sequencing, mass-spectrometry proteomics, and other global measurement technologies, along with the emergence of computational and systems biology, have facilitated huge strides in our ability to make meaningful observations at all levels of biological phenomena. As a result, we can now make comparative assessments of the degree to which various processes are conserved and connected between species. Additionally, newly developed techniques for highly efficient genome manipulation enable us to test these comparative observations experimentally. As an example, global measurement technologies have enabled observation of the abundances of nearly all proteins and mRNAs in the cells in many organisms, and further determine to what extent proteins levels are controlled by the levels of mRNA. In chapter one, I utilize these data to investigate the conservation of protein and mRNA abundance levels across a diverse set of organisms. Expression levels, while important for proper cell functioning, are only one observable property between genotype and phenotype. Evolutionary conservation can be seen all the way to phenotype, as in the identification of orthologous phenotypes, or ‘phenologs’, described in further detail in the introduction. Given these conserved phenomena, we asked a simple question: ’To what extent can an organisms genes function in the context of another organism?’. To address this question, we systematically replaced hundreds of yeast genes with their human orthologs. In chapters two and three I present these replacements, and further discuss what features of the genes or ortholog groups can explain their ability to replace or not. Chapter four includes a presentation of an ongoing effort to extend the replacement studies by replacing a complete yeast protein complex with its human counterpart. I will end with a discussion of the current state of relevant work, and where I see additional efforts going into the future. Advisors/Committee Members: Marcotte, Edward M. (advisor), Ellington, Andrew D (committee member), Iyer, Vishwanath R (committee member), Browning, Karen S (committee member), Appling, Dean R (committee member).

Subjects/Keywords: Humanization; Yeast genetics; Systems biology; Humanized yeast

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APA (6^th Edition):

Laurent, J. M. (2016). Evolutionary conservation of protein abundance and function. (Doctoral Dissertation). University of Texas – Austin. Retrieved from http://hdl.handle.net/2152/68224

Chicago Manual of Style (16^th Edition):

Laurent, Jon Michael. “Evolutionary conservation of protein abundance and function.” 2016. Doctoral Dissertation, University of Texas – Austin. Accessed January 20, 2021. http://hdl.handle.net/2152/68224.

MLA Handbook (7^th Edition):

Laurent, Jon Michael. “Evolutionary conservation of protein abundance and function.” 2016. Web. 20 Jan 2021.

Vancouver:

Laurent JM. Evolutionary conservation of protein abundance and function. [Internet] [Doctoral dissertation]. University of Texas – Austin; 2016. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/2152/68224.

Council of Science Editors:

Laurent JM. Evolutionary conservation of protein abundance and function. [Doctoral Dissertation]. University of Texas – Austin; 2016. Available from: http://hdl.handle.net/2152/68224

Dalhousie University

2. LeBlanc, Marissa. Regulation of Lipid Metabolism and Membrane Trafficking by the Oxysterol Binding Protein Superfamily Member Kes1.

Degree: PhD, Department of Biochemistry & Molecular Biology, 2010, Dalhousie University

URL: http://hdl.handle.net/10222/13033

► The Saccharomyces cerevisiae oxysterol binding protein homologue Kes1/Osh4 is a member of an enigmatic class of proteins found throughout Eukarya. This family of proteins is… (more)

▼ The Saccharomyces cerevisiae oxysterol binding protein homologue Kes1/Osh4 is a member of an enigmatic class of proteins found throughout Eukarya. This family of proteins is united by a ?-barrel structure that binds sterols and oxysterols. An N-terminal lid is thought to both sequester sterols inside the core and promote localization of Kes1 to regions of high membrane curvature via a predicted ArfGAP lipid packing sensor motif. Additionally, a phosphoinositide-binding region on a discrete surface of Kes1 has also been identified. In this thesis, structure-function analysis of Kes1 determined that phosphoinositide binding is required for membrane association in vitro, and in vivo phosphoinositide binding is required for localization to the Golgi. Ergosterol, the major sterol in S. cerevisiae, and membrane curvature had minimal effects on membrane association. This study also revealed a role for Kes1 in the regulation of both phosphatidylinositol-4-phosphate and phosphatidylinositol-3-phosphate homeostasis. Phosphoinositide and sterol binding by Kes1 are necessary for it to alter phosphatidylinositol-4-phosphate, but not phosphatidylinositol-3-phosphate homeostasis. Misregulation of phosphatidylinositol-4-phosphate homeostasis by Kes1 manifested itself in an inability of the v-SNARE Snc1 to traffic properly and was consistent with a defect in trans-Golgi/endosome trafficking. I went on to demonstrate a role for Kes1 in regulating the conversion of phosphatidylinositol-4-phosphate to phosphatidylinositol for the synthesis of sphingolipids, and I present a model for the role of Kes1 at the Golgi. Kes1 acts as a sterol sensor that regulates phosphatidylinositol-4-phosphate to sphingolipids metabolism, which ultimately regulates the delivery of proteins that assemble into lipid rafts for their transport from the Golgi to the plasma membrane. I also uncovered a previously unknown role for Kes1 in the regulation of the cytoplasm-to-vacuole and autophagy trafficking pathways, which is mediated by the ability of Kes1 to regulate phosphatidylinositol-3-phosphate homeostasis. Advisors/Committee Members: Dr. Lina Obeid (external-examiner), Dr. Richard Singer (graduate-coordinator), Dr. Richard Singer (thesis-reader), Dr. Roy Duncan (thesis-reader), Dr. David Byers (thesis-reader), Dr. Christopher McMaster (thesis-supervisor), Not Applicable (ethics-approval), Not Applicable (manuscripts), Yes (copyright-release).

Subjects/Keywords: lipid; vesicular trafficking; yeast genetics

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

LeBlanc, M. (2010). Regulation of Lipid Metabolism and Membrane Trafficking by the Oxysterol Binding Protein Superfamily Member Kes1. (Doctoral Dissertation). Dalhousie University. Retrieved from http://hdl.handle.net/10222/13033

Chicago Manual of Style (16^th Edition):

LeBlanc, Marissa. “Regulation of Lipid Metabolism and Membrane Trafficking by the Oxysterol Binding Protein Superfamily Member Kes1.” 2010. Doctoral Dissertation, Dalhousie University. Accessed January 20, 2021. http://hdl.handle.net/10222/13033.

MLA Handbook (7^th Edition):

LeBlanc, Marissa. “Regulation of Lipid Metabolism and Membrane Trafficking by the Oxysterol Binding Protein Superfamily Member Kes1.” 2010. Web. 20 Jan 2021.

Vancouver:

LeBlanc M. Regulation of Lipid Metabolism and Membrane Trafficking by the Oxysterol Binding Protein Superfamily Member Kes1. [Internet] [Doctoral dissertation]. Dalhousie University; 2010. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/10222/13033.

Council of Science Editors:

LeBlanc M. Regulation of Lipid Metabolism and Membrane Trafficking by the Oxysterol Binding Protein Superfamily Member Kes1. [Doctoral Dissertation]. Dalhousie University; 2010. Available from: http://hdl.handle.net/10222/13033

University of Utah

3. Schlesinger, Mylynda B. Studies of DNA replication in the yeast S; Cerevisiae;.

Degree: PhD, Biochemistry;, 2001, University of Utah

URL: http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/1334/rec/1259

► In this dissertation I present the contributions that I have made to two separate projects, each concerning different aspects of DNA replication in the yeast… (more)

Subjects/Keywords: Genetics; Yeast

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APA (6^th Edition):

Schlesinger, M. B. (2001). Studies of DNA replication in the yeast S; Cerevisiae;. (Doctoral Dissertation). University of Utah. Retrieved from http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/1334/rec/1259

Chicago Manual of Style (16^th Edition):

Schlesinger, Mylynda B. “Studies of DNA replication in the yeast S; Cerevisiae;.” 2001. Doctoral Dissertation, University of Utah. Accessed January 20, 2021. http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/1334/rec/1259.

MLA Handbook (7^th Edition):

Schlesinger, Mylynda B. “Studies of DNA replication in the yeast S; Cerevisiae;.” 2001. Web. 20 Jan 2021.

Vancouver:

Schlesinger MB. Studies of DNA replication in the yeast S; Cerevisiae;. [Internet] [Doctoral dissertation]. University of Utah; 2001. [cited 2021 Jan 20]. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/1334/rec/1259.

Council of Science Editors:

Schlesinger MB. Studies of DNA replication in the yeast S; Cerevisiae;. [Doctoral Dissertation]. University of Utah; 2001. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/1334/rec/1259

University of Adelaide

4. Val, Dale Lloyd Henry. Yeast pyruvate carboxylase 2 gene (PYC2) and structure-function studies on yeast pyc isozymes / by Dale Lloyd Henry Val.

Degree: 1995, University of Adelaide

URL: http://hdl.handle.net/2440/21609

Subjects/Keywords: Yeast Genetics.

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APA (6^th Edition):

Val, D. L. H. (1995). Yeast pyruvate carboxylase 2 gene (PYC2) and structure-function studies on yeast pyc isozymes / by Dale Lloyd Henry Val. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/21609

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Val, Dale Lloyd Henry. “Yeast pyruvate carboxylase 2 gene (PYC2) and structure-function studies on yeast pyc isozymes / by Dale Lloyd Henry Val.” 1995. Thesis, University of Adelaide. Accessed January 20, 2021. http://hdl.handle.net/2440/21609.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Val, Dale Lloyd Henry. “Yeast pyruvate carboxylase 2 gene (PYC2) and structure-function studies on yeast pyc isozymes / by Dale Lloyd Henry Val.” 1995. Web. 20 Jan 2021.

Vancouver:

Val DLH. Yeast pyruvate carboxylase 2 gene (PYC2) and structure-function studies on yeast pyc isozymes / by Dale Lloyd Henry Val. [Internet] [Thesis]. University of Adelaide; 1995. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/2440/21609.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Val DLH. Yeast pyruvate carboxylase 2 gene (PYC2) and structure-function studies on yeast pyc isozymes / by Dale Lloyd Henry Val. [Thesis]. University of Adelaide; 1995. Available from: http://hdl.handle.net/2440/21609

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Oxford

5. Feltham, Jack. Gene expression and regulation in the yeast metabolic cycle.

Degree: PhD, 2018, University of Oxford

URL: http://ora.ox.ac.uk/objects/uuid:73b2530f-92f8-459e-8eb9-9f57d20243be ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.770732

► Biological oscillations such as the circadian rhythm are central to physiology of organisms ranging from humans to bacteria. By coupling cellular processes to a periodic… (more)

Subjects/Keywords: Biochemistry; Yeast genetics; Molecular biology

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APA (6^th Edition):

Feltham, J. (2018). Gene expression and regulation in the yeast metabolic cycle. (Doctoral Dissertation). University of Oxford. Retrieved from http://ora.ox.ac.uk/objects/uuid:73b2530f-92f8-459e-8eb9-9f57d20243be ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.770732

Chicago Manual of Style (16^th Edition):

Feltham, Jack. “Gene expression and regulation in the yeast metabolic cycle.” 2018. Doctoral Dissertation, University of Oxford. Accessed January 20, 2021. http://ora.ox.ac.uk/objects/uuid:73b2530f-92f8-459e-8eb9-9f57d20243be ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.770732.

MLA Handbook (7^th Edition):

Feltham, Jack. “Gene expression and regulation in the yeast metabolic cycle.” 2018. Web. 20 Jan 2021.

Vancouver:

Feltham J. Gene expression and regulation in the yeast metabolic cycle. [Internet] [Doctoral dissertation]. University of Oxford; 2018. [cited 2021 Jan 20]. Available from: http://ora.ox.ac.uk/objects/uuid:73b2530f-92f8-459e-8eb9-9f57d20243be ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.770732.

Council of Science Editors:

Feltham J. Gene expression and regulation in the yeast metabolic cycle. [Doctoral Dissertation]. University of Oxford; 2018. Available from: http://ora.ox.ac.uk/objects/uuid:73b2530f-92f8-459e-8eb9-9f57d20243be ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.770732

Hong Kong University of Science and Technology

6. Sun, Xiao LIFS. Identification of the synthetic lethal mutations in yeast expressing a C-terminal truncation of the delta subunit (Ret2p) of COPI coatomer.

Degree: 2013, Hong Kong University of Science and Technology

URL: http://repository.ust.hk/ir/Record/1783.1-92648 ; https://doi.org/10.14711/thesis-b1256224 ; http://repository.ust.hk/ir/bitstream/1783.1-92648/1/th_redirect.html

► In the secretory pathway in Saccharomyces cerevisiae, the retrograde transport from Golgi to the ER is mediated by COPI coated vesicles. The COPI coat is… (more)

▼ In the secretory pathway in Saccharomyces cerevisiae, the retrograde transport from Golgi to the ER is mediated by COPI coated vesicles. The COPI coat is compromised of the heptameric complex (α-COP, β-COP, γ-COP, δ-COP, ε-COP and ζ-COP), termed coatomer, and the small GTPase Arf1. SNARE proteins are consist of a subset of vesicle cargo proteins and are important for specific vesicle fusion event. However, less is known for the interaction between the coatomer δ-COP (Ret2p) and the SNAREs. Sec22p is one of the SNAREs cycle between the ER and the Golgi apparatus. Previous data has show that amino acid substitutions in Sec26p/β-COP and Ret2p/δ-COP disrupt Sec22p’s Golgi localization. I obtained eight ret2 mutants that are defective in Sec22p retention and that are not temperature sensitive for growth. Surprisingly, a number of these mutants contained nonsense mutations (stop codons) in the ret2 coding region. These mutants indicate that the C-terminus of Ret2p is not essential for cell growth. To further investigate the requirement of the C-terminus of Ret2p, I generated incremental C-terminal truncations of the protein. Among the ret2 truncation mutants, ret2 (amino acids 1-120) cells were temperature-sensitive for growth and also significantly reduced steady-state levels of Ret2p relative to other RET2 mutants – as judged by immunoblotting. Based on these results, I speculate that Ret2p C-terminus (171a.a.-546 a.a.) is not essential for cell growth and the amino acids 120-170 of Ret2p are important for the essential function of Ret2p. In eukaryotic cells, the length and amino acid composition of the C-terminus of δ-COP (RET2) is evolutionarily conserved and the RET2 gene is essential for yeast cell growth. To gain deeper insight into the role of the C-terminus of Ret2p a synthetic lethal screen was carried out using with one of the truncations - ret2p ^(1-170). From the synthetic lethal screen I identified the GLO3 gene. Glo3p encodes a G̲TPase a̲ctivating p̲rotein (GAP) for the COPI GTPase Arf1p. Moreover, interactions between Glo3p and COPI coat (coatomer), vesicle cargos and SNAREs have been reported. In addition to its GAP domain, Glo3p also contains a cargo-binding domain termed BoCCS (Binding of Coatomer, Cargo and SNAREs) as well as the Glo3p-regulatory motif (GRM). Using site-directed mutagenesis and complementation analysis in yeast I established that the synthetic lethal relationship between ret2p^(1-170) and GLO3 results from the loss of Glo3p’s GAP activity.

Subjects/Keywords: Saccharomyces cerevisiae ; Genetics ; Yeast

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APA (6^th Edition):

Sun, X. L. (2013). Identification of the synthetic lethal mutations in yeast expressing a C-terminal truncation of the delta subunit (Ret2p) of COPI coatomer. (Thesis). Hong Kong University of Science and Technology. Retrieved from http://repository.ust.hk/ir/Record/1783.1-92648 ; https://doi.org/10.14711/thesis-b1256224 ; http://repository.ust.hk/ir/bitstream/1783.1-92648/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Sun, Xiao LIFS. “Identification of the synthetic lethal mutations in yeast expressing a C-terminal truncation of the delta subunit (Ret2p) of COPI coatomer.” 2013. Thesis, Hong Kong University of Science and Technology. Accessed January 20, 2021. http://repository.ust.hk/ir/Record/1783.1-92648 ; https://doi.org/10.14711/thesis-b1256224 ; http://repository.ust.hk/ir/bitstream/1783.1-92648/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Sun, Xiao LIFS. “Identification of the synthetic lethal mutations in yeast expressing a C-terminal truncation of the delta subunit (Ret2p) of COPI coatomer.” 2013. Web. 20 Jan 2021.

Vancouver:

Sun XL. Identification of the synthetic lethal mutations in yeast expressing a C-terminal truncation of the delta subunit (Ret2p) of COPI coatomer. [Internet] [Thesis]. Hong Kong University of Science and Technology; 2013. [cited 2021 Jan 20]. Available from: http://repository.ust.hk/ir/Record/1783.1-92648 ; https://doi.org/10.14711/thesis-b1256224 ; http://repository.ust.hk/ir/bitstream/1783.1-92648/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Sun XL. Identification of the synthetic lethal mutations in yeast expressing a C-terminal truncation of the delta subunit (Ret2p) of COPI coatomer. [Thesis]. Hong Kong University of Science and Technology; 2013. Available from: http://repository.ust.hk/ir/Record/1783.1-92648 ; https://doi.org/10.14711/thesis-b1256224 ; http://repository.ust.hk/ir/bitstream/1783.1-92648/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Victoria University of Wellington

7. Sampson, Liam D P. High Throughput Drug Discovery in S. Cerevisiae: the Characterisation of FC-592 and FC-888.

Degree: 2012, Victoria University of Wellington

URL: http://hdl.handle.net/10063/2590

► The discovery and characterisation of novel small molecule drug candidates is a medical priority. Recent advances in synthetic organic chemistry allow the de novo production… (more)

▼ The discovery and characterisation of novel small molecule drug candidates is a medical priority. Recent advances in synthetic organic chemistry allow the de novo production of diversity oriented synthetic compound libraries and synthetic modification of natural products to provide candidate compounds for screening as potential therapeutics, bioactive agents or genetic probes. Small drugs function through interaction with complex genetic networks and pathways. However, it is difficult to characterise these interactions on a genome wide level to achieve understanding of drug mechanism. Here, discovery based approaches are utilised to achieve system wide parsing of biological mechanism, in an attempt to characterise the action of novel synthetic compounds and natural product derivatives. Chemical genomic analysis allows for such understanding by examining growth profiles of a genomic deletion library of Saccharomyces cerevisiae mutants in the presence of sub-inhibitory concentrations of drug. The gene targets of small molecule compounds can be identified by noting deletion strains which display increased sensitivity, indicating chemical interaction with the associated gene network. In addition, the development and characterisation of resistant mutants can be used to identify putative drug targets. In this strategy, characterisation of the mechanism of resistance gives insight into drug mode-of-action. This study develops a high throughput yeast inhibition assay to identify bioactive compounds from a synthetic organic compound library, and attempts to characterise mechanism of action by establishing a profile of each compound’s interaction with these gene networks; and mapping a resistance mutation to provide evidence of inhibitory mechanism. Two candidate compounds are identified, FC-592 and FC-888. FC-592 displayed cytostatic inhibition. Further, yeast tag microarray homozygous profiling (HOP), chemical structure analysis, and cell-cycle analysis via flow cytometry for this compound provided evidence for a mechanism of poor specificity that targets glycoprotein biosynthesis and the secretory (Sec) pathway, as well as the cell-division cycle (CDC) pathway. Attempts to characterise a mutant resistant to this compound via synthetic genetic array mapping were unsuccessful when the resistance mutation proved to mediate a slow growth phenotype, abrogating the Synthetic Genetic Array Mapping approach utilised. Pending further analysis, it is suggested that this compound could have a role as a genetic probe in future exploration of the Sec and CDC pathways. Chemical structure analysis and a non-specific HOP screen chemigenomic profile suggested that FC-888 is an alkylating agent with a broad affinity for cellular nucleophiles. The compound demonstrates cytotoxic activity, and its efflux is not mediated by the pleiotropic drug resistance (PDR) network. It is suggested that the compound could find utility as a probe dissecting processes related to cellular defence against non-DNA specific alkylation. Advisors/Committee Members: Atkinson, Paul, Bellows, David.

Subjects/Keywords: Drug discovery; Chemical genetics; Yeast

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APA (6^th Edition):

Sampson, L. D. P. (2012). High Throughput Drug Discovery in S. Cerevisiae: the Characterisation of FC-592 and FC-888. (Masters Thesis). Victoria University of Wellington. Retrieved from http://hdl.handle.net/10063/2590

Chicago Manual of Style (16^th Edition):

Sampson, Liam D P. “High Throughput Drug Discovery in S. Cerevisiae: the Characterisation of FC-592 and FC-888.” 2012. Masters Thesis, Victoria University of Wellington. Accessed January 20, 2021. http://hdl.handle.net/10063/2590.

MLA Handbook (7^th Edition):

Sampson, Liam D P. “High Throughput Drug Discovery in S. Cerevisiae: the Characterisation of FC-592 and FC-888.” 2012. Web. 20 Jan 2021.

Vancouver:

Sampson LDP. High Throughput Drug Discovery in S. Cerevisiae: the Characterisation of FC-592 and FC-888. [Internet] [Masters thesis]. Victoria University of Wellington; 2012. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/10063/2590.

Council of Science Editors:

Sampson LDP. High Throughput Drug Discovery in S. Cerevisiae: the Characterisation of FC-592 and FC-888. [Masters Thesis]. Victoria University of Wellington; 2012. Available from: http://hdl.handle.net/10063/2590

University of Toronto

8. Li, Zhijian. Systematic Exploration of Essential Yeast Gene Functions with Temperature-sensitive Mutants.

Degree: 2011, University of Toronto

URL: http://hdl.handle.net/1807/29790

► The budding yeast Saccharomyces cerevisiae is the most well characterized model organism for systematic analysis of fundamental eukaryotic processes. Approximately 19% of S. cerevisiae genes… (more)

▼ The budding yeast Saccharomyces cerevisiae is the most well characterized model organism for systematic analysis of fundamental eukaryotic processes. Approximately 19% of S. cerevisiae genes are considered essential. Essential genes tend to be more highly conserved from yeast to humans when compared to nonessential genes. The set of essential yeast genes spans diverse biological processes and while the primary role of most essential yeast genes has been characterized, the full breadth of function associated with essential genes has not been examined, due, at least in part, to the lack of adequate genetic reagents for their conditional and systematic perturbations. To systematically study yeast essential gene functions using synthetic genetic array analysis and to complement the current yeast deletion collection, I constructed a collection of temperature-sensitive yeast mutants consisting of 795 ts strains, covering 501 (~45%) of the 1,101 essential yeast genes, with ~30% of the genes represented by multiple alleles. This is the largest collection of isogenic ts yeast mutants constructed to date. I confirmed the correct integration of over 99% of the ts alleles using PCR-based strategy and the identity of the ts allele by complementation of the ts phenotype with its cognate plasmid. The ts mutant collection was characterized by high-resolution profiling of the temperature sensitivity of each ts strain, distribution analysis of gene ontology molecular function and biological process, and comparison of ts allele strains to the strains carrying Tet-repressible alleles of essential genes. The results demonstrated that the ts collection is a powerful reagent for the systematic study of yeast essential gene functions and provides a valuable resource to complement the current yeast deletion collection. I validated and demonstrated the usefulness of the ts collection in a number of different ways. First, I carried out detailed temperature profiling of each mutant strain using liquid growth assays and found that ts mutants that define particular biological pathways often show highly similar profiles. Second, I showed that the ts mutant array can be used to screen compounds for suppression of growth defects and thus is useful for exploration of chemical-genetic interactions. Third, I demonstrated that the ts collection represents a key reagent set for genetic interaction analysis because essential genes tend to be highly connected hubs on the global genetic network. Fourth, I further validated the ts array as a key resource for quantitative phenotypic analysis by using a high-content screening protocol to score six different fluorescent markers, diagnostic for different subcellular compartments or structures, in hundreds of different mutants. Quantification of the marker behaviour at the single-cell level enabled integration of this data set to generate a morphological profile for each ts mutant to reveal both known and previously unappreciated functions for essential genes, including roles for cohesion and condensin genes… Advisors/Committee Members: Boone, Charles, Molecular and Medical Genetics.

Subjects/Keywords: Genetics; Yeast; 0369; 0307

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Li, Z. (2011). Systematic Exploration of Essential Yeast Gene Functions with Temperature-sensitive Mutants. (Doctoral Dissertation). University of Toronto. Retrieved from http://hdl.handle.net/1807/29790

Chicago Manual of Style (16^th Edition):

Li, Zhijian. “Systematic Exploration of Essential Yeast Gene Functions with Temperature-sensitive Mutants.” 2011. Doctoral Dissertation, University of Toronto. Accessed January 20, 2021. http://hdl.handle.net/1807/29790.

MLA Handbook (7^th Edition):

Li, Zhijian. “Systematic Exploration of Essential Yeast Gene Functions with Temperature-sensitive Mutants.” 2011. Web. 20 Jan 2021.

Vancouver:

Li Z. Systematic Exploration of Essential Yeast Gene Functions with Temperature-sensitive Mutants. [Internet] [Doctoral dissertation]. University of Toronto; 2011. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/1807/29790.

Council of Science Editors:

Li Z. Systematic Exploration of Essential Yeast Gene Functions with Temperature-sensitive Mutants. [Doctoral Dissertation]. University of Toronto; 2011. Available from: http://hdl.handle.net/1807/29790

Michigan State University

9. Hsu, Wen Hwei. Construction of new cloning vectors for the genetic manipulation of yeasts.

Degree: PhD, Department of Microbiology and Public Health, 1983, Michigan State University

URL: http://etd.lib.msu.edu/islandora/object/etd:18285

Subjects/Keywords: Yeast – Genetics; Yeast; Cloning

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hsu, W. H. (1983). Construction of new cloning vectors for the genetic manipulation of yeasts. (Doctoral Dissertation). Michigan State University. Retrieved from http://etd.lib.msu.edu/islandora/object/etd:18285

Chicago Manual of Style (16^th Edition):

Hsu, Wen Hwei. “Construction of new cloning vectors for the genetic manipulation of yeasts.” 1983. Doctoral Dissertation, Michigan State University. Accessed January 20, 2021. http://etd.lib.msu.edu/islandora/object/etd:18285.

MLA Handbook (7^th Edition):

Hsu, Wen Hwei. “Construction of new cloning vectors for the genetic manipulation of yeasts.” 1983. Web. 20 Jan 2021.

Vancouver:

Hsu WH. Construction of new cloning vectors for the genetic manipulation of yeasts. [Internet] [Doctoral dissertation]. Michigan State University; 1983. [cited 2021 Jan 20]. Available from: http://etd.lib.msu.edu/islandora/object/etd:18285.

Council of Science Editors:

Hsu WH. Construction of new cloning vectors for the genetic manipulation of yeasts. [Doctoral Dissertation]. Michigan State University; 1983. Available from: http://etd.lib.msu.edu/islandora/object/etd:18285

Columbia University

10. Yang, Jamie Siyu. Yeast adaptation and survival under acute exposure to lethal ethanol stress.

Degree: 2020, Columbia University

URL: https://doi.org/10.7916/d8-q320-8589

► The ability to respond to stress is universal in all domains of life. Failure to properly execute the stress response compromises the fitness of the… (more)

Subjects/Keywords: Biology; Genetics; Stress (Physiology); Adaptation (Biology); Yeast

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APA (6^th Edition):

Yang, J. S. (2020). Yeast adaptation and survival under acute exposure to lethal ethanol stress. (Doctoral Dissertation). Columbia University. Retrieved from https://doi.org/10.7916/d8-q320-8589

Chicago Manual of Style (16^th Edition):

Yang, Jamie Siyu. “Yeast adaptation and survival under acute exposure to lethal ethanol stress.” 2020. Doctoral Dissertation, Columbia University. Accessed January 20, 2021. https://doi.org/10.7916/d8-q320-8589.

MLA Handbook (7^th Edition):

Yang, Jamie Siyu. “Yeast adaptation and survival under acute exposure to lethal ethanol stress.” 2020. Web. 20 Jan 2021.

Vancouver:

Yang JS. Yeast adaptation and survival under acute exposure to lethal ethanol stress. [Internet] [Doctoral dissertation]. Columbia University; 2020. [cited 2021 Jan 20]. Available from: https://doi.org/10.7916/d8-q320-8589.

Council of Science Editors:

Yang JS. Yeast adaptation and survival under acute exposure to lethal ethanol stress. [Doctoral Dissertation]. Columbia University; 2020. Available from: https://doi.org/10.7916/d8-q320-8589

University of Adelaide

11. [No author]. Mitochondrial genetics of yeast / by David F. Callen.

Degree: 1975, University of Adelaide

URL: http://hdl.handle.net/2440/20031

Subjects/Keywords: Mitochondrial genetics.; Yeast.

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APA (6^th Edition):

author], [. (1975). Mitochondrial genetics of yeast / by David F. Callen. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/20031

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

author], [No. “Mitochondrial genetics of yeast / by David F. Callen.” 1975. Thesis, University of Adelaide. Accessed January 20, 2021. http://hdl.handle.net/2440/20031.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

author], [No. “Mitochondrial genetics of yeast / by David F. Callen.” 1975. Web. 20 Jan 2021.

Vancouver:

author] [. Mitochondrial genetics of yeast / by David F. Callen. [Internet] [Thesis]. University of Adelaide; 1975. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/2440/20031.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

author] [. Mitochondrial genetics of yeast / by David F. Callen. [Thesis]. University of Adelaide; 1975. Available from: http://hdl.handle.net/2440/20031

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Heriot-Watt University

12. Comerford, John G. Magnesium and the plasma membrane adenosine triphosphatase in cell cycle mutants of fission yeast.

Degree: PhD, 1985, Heriot-Watt University

URL: http://hdl.handle.net/10399/1609

Subjects/Keywords: 572.8; Yeast genetics

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APA (6^th Edition):

Comerford, J. G. (1985). Magnesium and the plasma membrane adenosine triphosphatase in cell cycle mutants of fission yeast. (Doctoral Dissertation). Heriot-Watt University. Retrieved from http://hdl.handle.net/10399/1609

Chicago Manual of Style (16^th Edition):

Comerford, John G. “Magnesium and the plasma membrane adenosine triphosphatase in cell cycle mutants of fission yeast.” 1985. Doctoral Dissertation, Heriot-Watt University. Accessed January 20, 2021. http://hdl.handle.net/10399/1609.

MLA Handbook (7^th Edition):

Comerford, John G. “Magnesium and the plasma membrane adenosine triphosphatase in cell cycle mutants of fission yeast.” 1985. Web. 20 Jan 2021.

Vancouver:

Comerford JG. Magnesium and the plasma membrane adenosine triphosphatase in cell cycle mutants of fission yeast. [Internet] [Doctoral dissertation]. Heriot-Watt University; 1985. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/10399/1609.

Council of Science Editors:

Comerford JG. Magnesium and the plasma membrane adenosine triphosphatase in cell cycle mutants of fission yeast. [Doctoral Dissertation]. Heriot-Watt University; 1985. Available from: http://hdl.handle.net/10399/1609

University of Bath

13. Evans, David Roy Hywel. The dna26-1 mutation of Saccharomyces cerevisiae.

Degree: PhD, 1991, University of Bath

URL: https://researchportal.bath.ac.uk/en/studentthesis/the-dna261-mutation-of-saccharomyces-cerevisiae(4877e73f-5288-426d-b10f-d1c07b042f06).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.280897

Subjects/Keywords: 572.8; Yeast genetics

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APA (6^th Edition):

Evans, D. R. H. (1991). The dna26-1 mutation of Saccharomyces cerevisiae. (Doctoral Dissertation). University of Bath. Retrieved from https://researchportal.bath.ac.uk/en/studentthesis/the-dna261-mutation-of-saccharomyces-cerevisiae(4877e73f-5288-426d-b10f-d1c07b042f06).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.280897

Chicago Manual of Style (16^th Edition):

Evans, David Roy Hywel. “The dna26-1 mutation of Saccharomyces cerevisiae.” 1991. Doctoral Dissertation, University of Bath. Accessed January 20, 2021. https://researchportal.bath.ac.uk/en/studentthesis/the-dna261-mutation-of-saccharomyces-cerevisiae(4877e73f-5288-426d-b10f-d1c07b042f06).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.280897.

MLA Handbook (7^th Edition):

Evans, David Roy Hywel. “The dna26-1 mutation of Saccharomyces cerevisiae.” 1991. Web. 20 Jan 2021.

Vancouver:

Evans DRH. The dna26-1 mutation of Saccharomyces cerevisiae. [Internet] [Doctoral dissertation]. University of Bath; 1991. [cited 2021 Jan 20]. Available from: https://researchportal.bath.ac.uk/en/studentthesis/the-dna261-mutation-of-saccharomyces-cerevisiae(4877e73f-5288-426d-b10f-d1c07b042f06).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.280897.

Council of Science Editors:

Evans DRH. The dna26-1 mutation of Saccharomyces cerevisiae. [Doctoral Dissertation]. University of Bath; 1991. Available from: https://researchportal.bath.ac.uk/en/studentthesis/the-dna261-mutation-of-saccharomyces-cerevisiae(4877e73f-5288-426d-b10f-d1c07b042f06).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.280897

Hong Kong University of Science and Technology

14. Chen, Li LIFS. Mutations in TED1 and DCR2, two glycosylphosphatidylinositol anchored protein remodelases, activate the spindle assembly checkpoint in budding yeast cells.

Degree: 2017, Hong Kong University of Science and Technology

URL: http://repository.ust.hk/ir/Record/1783.1-105622 ; https://doi.org/10.14711/thesis-991012564169103412 ; http://repository.ust.hk/ir/bitstream/1783.1-105622/1/th_redirect.html

► Herein I describe a genetic study that employed a temperature-sensitive allele encoding the Golgi glycosyltransferase sorting receptor VPS74. Through characterizing genes that act as dosage… (more)

Subjects/Keywords: Yeast ; Genetics ; Growth ; Proteins ; Cell receptors

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APA (6^th Edition):

Chen, L. L. (2017). Mutations in TED1 and DCR2, two glycosylphosphatidylinositol anchored protein remodelases, activate the spindle assembly checkpoint in budding yeast cells. (Thesis). Hong Kong University of Science and Technology. Retrieved from http://repository.ust.hk/ir/Record/1783.1-105622 ; https://doi.org/10.14711/thesis-991012564169103412 ; http://repository.ust.hk/ir/bitstream/1783.1-105622/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Chen, Li LIFS. “Mutations in TED1 and DCR2, two glycosylphosphatidylinositol anchored protein remodelases, activate the spindle assembly checkpoint in budding yeast cells.” 2017. Thesis, Hong Kong University of Science and Technology. Accessed January 20, 2021. http://repository.ust.hk/ir/Record/1783.1-105622 ; https://doi.org/10.14711/thesis-991012564169103412 ; http://repository.ust.hk/ir/bitstream/1783.1-105622/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Chen, Li LIFS. “Mutations in TED1 and DCR2, two glycosylphosphatidylinositol anchored protein remodelases, activate the spindle assembly checkpoint in budding yeast cells.” 2017. Web. 20 Jan 2021.

Vancouver:

Chen LL. Mutations in TED1 and DCR2, two glycosylphosphatidylinositol anchored protein remodelases, activate the spindle assembly checkpoint in budding yeast cells. [Internet] [Thesis]. Hong Kong University of Science and Technology; 2017. [cited 2021 Jan 20]. Available from: http://repository.ust.hk/ir/Record/1783.1-105622 ; https://doi.org/10.14711/thesis-991012564169103412 ; http://repository.ust.hk/ir/bitstream/1783.1-105622/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Chen LL. Mutations in TED1 and DCR2, two glycosylphosphatidylinositol anchored protein remodelases, activate the spindle assembly checkpoint in budding yeast cells. [Thesis]. Hong Kong University of Science and Technology; 2017. Available from: http://repository.ust.hk/ir/Record/1783.1-105622 ; https://doi.org/10.14711/thesis-991012564169103412 ; http://repository.ust.hk/ir/bitstream/1783.1-105622/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Victoria University of Wellington

15. Busby, Bede Phillip. Phenotypic implications of genetic interaction networks in Saccharomyces cerevisiae.

Degree: 2015, Victoria University of Wellington

URL: http://hdl.handle.net/10063/8756

► Gene functions were studied as extensive networks comprising synergistic functional interactions between overlapping pairs of genes. Elucidation of such networks related to drug phenotypes (statins… (more)

▼ Gene functions were studied as extensive networks comprising synergistic functional interactions between overlapping pairs of genes. Elucidation of such networks related to drug phenotypes (statins in this thesis) provides additional information to classical genetics as to what genes and metabolic pathways might be involved in phenotypes and, importantly, where side-effects might arise in drug effects. A key question is whether there are genetic interaction networks that vary with individuals and with phenotypes. To answer this question a panel of twenty-six fully sequenced yeast strains from the Saccharomyces Genome Resequencing Project (SGRP; Sanger Institute) was screened for statin resistance to approximate a model for individuals in a human population. Three strains (Y55, SK1 and YPS606) were shown to be 500% more resistant to atorvastatin than the S288C laboratory control strain and were selected for further analysis. Synthetic genetic array analysis (SGA) and chemical genetic profiling were utilised to elucidate genetic interaction networks in the four different strains. SGA analysis depends on the availability of a genome-wide deletion mutant array (DMA) which already exists for S288C and the current studies constructed equivalent Y55-, SK1-, and YS606-strain specific deletion mutant arrays called here “ssDMA’s”. Creating the new ssDMAs involved six back-crossings (1-1/2⁶) of Y55, SK1 and YPS606 with S288C to place the genome-wide deletion mutations of S288C on the genetic background of the strains using appropriate selection markers between each backcrossing. The four DMAs were then subjected to chemical genetic profiling with two statin drugs and also subjected to SGA analysis utilising five query genes chosen for their involvement with the cellular response to statins. The query genes HMG1, HMG2, ARV1, BTS1 and OPI3 were constructed to be strain specific and generated a total 25 genetic interaction networks. The chemical genetic profiles in the ssDMAs identified off-target interactions genes associated with the resistance phenotype in Y55, SK1, and YPS606 that were not observed to show genetic interactions in the more sensitive S288c strain. There was little conservation of the genetic interaction networks elicited by the specific query genes between the strains with the exception of OPI3. There was, however, conservation of fundamental cellular processes, as might be expected, but the genes encoding these processes in the SGAs of the different strains were for the most part different. Therefore, we conclude that the genetic interaction networks concerning statins are different between individuals. Advisors/Committee Members: Atkinson, Paul, Teesdale-Spittle, Paul.

Subjects/Keywords: Genetic interaction; Network; Genetics; Saccharomyces cerevisiae; Yeast

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APA (6^th Edition):

Busby, B. P. (2015). Phenotypic implications of genetic interaction networks in Saccharomyces cerevisiae. (Doctoral Dissertation). Victoria University of Wellington. Retrieved from http://hdl.handle.net/10063/8756

Chicago Manual of Style (16^th Edition):

Busby, Bede Phillip. “Phenotypic implications of genetic interaction networks in Saccharomyces cerevisiae.” 2015. Doctoral Dissertation, Victoria University of Wellington. Accessed January 20, 2021. http://hdl.handle.net/10063/8756.

MLA Handbook (7^th Edition):

Busby, Bede Phillip. “Phenotypic implications of genetic interaction networks in Saccharomyces cerevisiae.” 2015. Web. 20 Jan 2021.

Vancouver:

Busby BP. Phenotypic implications of genetic interaction networks in Saccharomyces cerevisiae. [Internet] [Doctoral dissertation]. Victoria University of Wellington; 2015. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/10063/8756.

Council of Science Editors:

Busby BP. Phenotypic implications of genetic interaction networks in Saccharomyces cerevisiae. [Doctoral Dissertation]. Victoria University of Wellington; 2015. Available from: http://hdl.handle.net/10063/8756

Kansas State University

16. Riley, Michael I. Genetic analysis of trisomic tetraploids and the expression of crytopleurine resistance in aneuploid Saccharomyces cerevisiae.

Degree: 1976, Kansas State University

URL: http://hdl.handle.net/2097/11432

Subjects/Keywords: Fungi – Genetics; Yeast

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APA (6^th Edition):

Riley, M. I. (1976). Genetic analysis of trisomic tetraploids and the expression of crytopleurine resistance in aneuploid Saccharomyces cerevisiae. (Thesis). Kansas State University. Retrieved from http://hdl.handle.net/2097/11432

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Riley, Michael I. “Genetic analysis of trisomic tetraploids and the expression of crytopleurine resistance in aneuploid Saccharomyces cerevisiae.” 1976. Thesis, Kansas State University. Accessed January 20, 2021. http://hdl.handle.net/2097/11432.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Riley, Michael I. “Genetic analysis of trisomic tetraploids and the expression of crytopleurine resistance in aneuploid Saccharomyces cerevisiae.” 1976. Web. 20 Jan 2021.

Vancouver:

Riley MI. Genetic analysis of trisomic tetraploids and the expression of crytopleurine resistance in aneuploid Saccharomyces cerevisiae. [Internet] [Thesis]. Kansas State University; 1976. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/2097/11432.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Riley MI. Genetic analysis of trisomic tetraploids and the expression of crytopleurine resistance in aneuploid Saccharomyces cerevisiae. [Thesis]. Kansas State University; 1976. Available from: http://hdl.handle.net/2097/11432

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Leicester

17. Hu, Yue. Quantitative genetics of complex traits : solutions for studying the genetic basis of variation in yeast.

Degree: PhD, 2020, University of Leicester

URL: https://doi.org/10.25392/leicester.data.12651731.v1 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.811572

► Recent advances in high-throughput techniques for DNA sequencing and phenotyping have greatly facilitated the identification of genetic variants underlying traits at a genomewide level. In… (more)

▼ Recent advances in high-throughput techniques for DNA sequencing and phenotyping have greatly facilitated the identification of genetic variants underlying traits at a genomewide level. In this study, a large amount of yeast genetic resources and phenotypic data were collected for the study of natural genetic variation in yeast under different environment conditions. Quantitative trait locus (QTL) analysis and epistasis analysis have been applied to Saccharomyces cerevisiae on 6 groups of 1st generation bi-parental inter-cross segregants and 12th generation multiparental high resolution segregants. Using yeast as model organism, growth under stress conditions of a variety of conventional genotoxic agents was measured. Different QTLs were mapped to causative genes that are related to DNA repair and protein transport. In addition, by comparing the genes identified under 19 different agents, 14 frequently occurring genes producing effect on the growth of yeast, were further analysed. QTL output was clustered through a changepoint model for improving the selection of candidate genes in large gene sets. Furthermore, Temporal QTL analysis was applied to study the dynamic development of yeast growth under X-ray irradiation that expands the phenotype in the time dimension. By comparing the QTL in different time spans, genes that only exhibit effects for a certain period of time rather than continuously through, or at the end of, the experiment were found. One of the major industrial applications of yeast is brewing. In this project, whole genome sequencing analysis were performed on a highly diverse 12th generation de novo hybrid population. Variant calling was applied for these pool sequencing and identification of genetic variants. Pool QTL analysis was applied to compare the allele frequency difference of extreme pools under the same condition. Multiple QTL intervals responding to the brewing environment were identified. This provides useful genetic insights for brewing yeast breeding and improvement.

Subjects/Keywords: microbiology; yeast; complex traits; Quantitative genetics

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APA (6^th Edition):

Hu, Y. (2020). Quantitative genetics of complex traits : solutions for studying the genetic basis of variation in yeast. (Doctoral Dissertation). University of Leicester. Retrieved from https://doi.org/10.25392/leicester.data.12651731.v1 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.811572

Chicago Manual of Style (16^th Edition):

Hu, Yue. “Quantitative genetics of complex traits : solutions for studying the genetic basis of variation in yeast.” 2020. Doctoral Dissertation, University of Leicester. Accessed January 20, 2021. https://doi.org/10.25392/leicester.data.12651731.v1 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.811572.

MLA Handbook (7^th Edition):

Hu, Yue. “Quantitative genetics of complex traits : solutions for studying the genetic basis of variation in yeast.” 2020. Web. 20 Jan 2021.

Vancouver:

Hu Y. Quantitative genetics of complex traits : solutions for studying the genetic basis of variation in yeast. [Internet] [Doctoral dissertation]. University of Leicester; 2020. [cited 2021 Jan 20]. Available from: https://doi.org/10.25392/leicester.data.12651731.v1 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.811572.

Council of Science Editors:

Hu Y. Quantitative genetics of complex traits : solutions for studying the genetic basis of variation in yeast. [Doctoral Dissertation]. University of Leicester; 2020. Available from: https://doi.org/10.25392/leicester.data.12651731.v1 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.811572

Simon Fraser University

18. Adames, Neil R. Analysis of new genes involved in yeast mating and cell polarity.

Degree: 1997, Simon Fraser University

URL: http://summit.sfu.ca/item/7458

Subjects/Keywords: Yeast fungi – Genetics.

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APA (6^th Edition):

Adames, N. R. (1997). Analysis of new genes involved in yeast mating and cell polarity. (Thesis). Simon Fraser University. Retrieved from http://summit.sfu.ca/item/7458

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Adames, Neil R. “Analysis of new genes involved in yeast mating and cell polarity.” 1997. Thesis, Simon Fraser University. Accessed January 20, 2021. http://summit.sfu.ca/item/7458.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Adames, Neil R. “Analysis of new genes involved in yeast mating and cell polarity.” 1997. Web. 20 Jan 2021.

Vancouver:

Adames NR. Analysis of new genes involved in yeast mating and cell polarity. [Internet] [Thesis]. Simon Fraser University; 1997. [cited 2021 Jan 20]. Available from: http://summit.sfu.ca/item/7458.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Adames NR. Analysis of new genes involved in yeast mating and cell polarity. [Thesis]. Simon Fraser University; 1997. Available from: http://summit.sfu.ca/item/7458

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Washington

19. Andrie, Jennifer Margaret. A cradle-to-grave analysis of cis-regulatory variation in yeast.

Degree: PhD, 2017, University of Washington

URL: http://hdl.handle.net/1773/38629

► Cis-regulatory variation is an important source of phenotypic variation within populations and a major target of adaptive divergence between species. However, the molecular processes that… (more)

Subjects/Keywords: cis-regulatory; functional genomics; genome assembly; RNA decay; yeast; Genetics; Genetics

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APA (6^th Edition):

Andrie, J. M. (2017). A cradle-to-grave analysis of cis-regulatory variation in yeast. (Doctoral Dissertation). University of Washington. Retrieved from http://hdl.handle.net/1773/38629

Chicago Manual of Style (16^th Edition):

Andrie, Jennifer Margaret. “A cradle-to-grave analysis of cis-regulatory variation in yeast.” 2017. Doctoral Dissertation, University of Washington. Accessed January 20, 2021. http://hdl.handle.net/1773/38629.

MLA Handbook (7^th Edition):

Andrie, Jennifer Margaret. “A cradle-to-grave analysis of cis-regulatory variation in yeast.” 2017. Web. 20 Jan 2021.

Vancouver:

Andrie JM. A cradle-to-grave analysis of cis-regulatory variation in yeast. [Internet] [Doctoral dissertation]. University of Washington; 2017. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/1773/38629.

Council of Science Editors:

Andrie JM. A cradle-to-grave analysis of cis-regulatory variation in yeast. [Doctoral Dissertation]. University of Washington; 2017. Available from: http://hdl.handle.net/1773/38629

Rutgers University

20. Basu, Urmimala. Mechanism and regulation of yeast and human mitochondrial DNA transcription.

Degree: PhD, Transcription, 2019, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/60589/

► Mitochondria are autonomous double-membrane-bound organelles in eukaryotic cells. Their most important function is to synthesize ATP to meet the energy needs of the organism through… (more)

▼ Mitochondria are autonomous double-membrane-bound organelles in eukaryotic cells. Their most important function is to synthesize ATP to meet the energy needs of the organism through oxidative phosphorylation. This cardinal role in energy production makes mitochondria a key player in metabolic, degenerative, and age-related diseases. Dysregulations of mitochondrial energy production in humans affect all organs, but the high energy demanding organs of the body like the heart, brain, kidneys, etc., are the primary targets of mitochondrial malfunctions. Mitochondria contain their own genome that is replicated and transcribed by enzymes distinct from the nuclear enzymes. Interestingly, the mitochondrial DNA (mtDNA) replication and transcription enzymes are homologous to bacteriophage T7 encoded enzymes. Thus, yeast and human mtDNA are transcribed by phage T7-like single-subunit RNA polymerases (RNAP) called Rpo41 and POLRMT, respectively. They are structurally homologous to T7 RNAP, but both yeast and human RNAPs require transcription factors to initiate transcription which includes: Mtf1 (in yeast) and mitochondrial transcription factor A /TFAM and mitochondrial transcription factor B2 /TFB2M (in humans). Reliance of the mtRNAP on transcription factors, results in regulation of gene expression that is unprecedented in homologous phage T7. Transcription initiation is a crucial step where gene expression is regulated. However, mtDNA transcription initiation, elongation, and termination mechanisms are understudied. The overarching goals of my graduate research were to investigate the mechanisms of regulation of transcription initiation through biochemical and biophysical characterization. In the first part of the thesis, I have showed that yeast and human mtRNAPs can initiate transcription with NAD+/NADH, which results in production of capped RNA transcripts in the mitochondria. Capping with NAD+/NADH is a new discovery brought to light in the last decade. My research has showed that initiation with NAD+ or NADH is up to 40% as efficient as initiation with ATP for S. cerevisiae mtRNAP and up to 60% as efficient as initiation with ATP for human mtRNAP. Similarly, direct quantitation of NAD+- and NADH-capped RNA in vivo showed up to ~60% NAD+ and NADH capping of yeast mitochondrial transcripts and up to ~10% NAD+ capping of human mitochondrial transcripts. Furthermore, both S. cerevisiae mtRNAP and human mtRNAP can cap RNA with NAD+ and NADH more efficiently than bacterial RNAP and eukaryotic nuclear RNAP II. The capping efficiency is higher with promoter derivatives having R:Y at position -1 than with promoter derivatives having Y:R. The implications of alternatively capping mitochondrial RNAs are not known; however, capping can affect RNA stability, processing, and global gene expression. Since intracellular NAD+ and NADH levels dictate the efficiency of capping, we propose that mtRNAPs use NAD+/NADH capping as both a sensor and actuator in coupling cellular metabolism to mitochondrial transcriptional outputs, sensing NAD+… Advisors/Committee Members: Hampsey, Michael (chair), School of Graduate Studies.

Subjects/Keywords: Biochemistry; Genetic transcription; Yeast fungi – Genetics; Mitochondrial DNA – Genetics

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Basu, U. (2019). Mechanism and regulation of yeast and human mitochondrial DNA transcription. (Doctoral Dissertation). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/60589/

Chicago Manual of Style (16^th Edition):

Basu, Urmimala. “Mechanism and regulation of yeast and human mitochondrial DNA transcription.” 2019. Doctoral Dissertation, Rutgers University. Accessed January 20, 2021. https://rucore.libraries.rutgers.edu/rutgers-lib/60589/.

MLA Handbook (7^th Edition):

Basu, Urmimala. “Mechanism and regulation of yeast and human mitochondrial DNA transcription.” 2019. Web. 20 Jan 2021.

Vancouver:

Basu U. Mechanism and regulation of yeast and human mitochondrial DNA transcription. [Internet] [Doctoral dissertation]. Rutgers University; 2019. [cited 2021 Jan 20]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/60589/.

Council of Science Editors:

Basu U. Mechanism and regulation of yeast and human mitochondrial DNA transcription. [Doctoral Dissertation]. Rutgers University; 2019. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/60589/

21. Suleeporn Kitcha. Screening of Oleaginous Yeasts and Optimization for Lipid Production Using Crude Glycerol as a Carbon Source .

Degree: คณะอุตสาหกรรมเกษตร ภาควิชาเทคโนโลยีชีวภาพอุตสาหกรรม, 2012, Prince of Songkla University

URL: http://kb.psu.ac.th/psukb/handle/2016/10398

Subjects/Keywords: Lipids; Yeast; Yeast fungi Genetics; Biotechnology

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APA (6^th Edition):

Kitcha, S. (2012). Screening of Oleaginous Yeasts and Optimization for Lipid Production Using Crude Glycerol as a Carbon Source . (Thesis). Prince of Songkla University. Retrieved from http://kb.psu.ac.th/psukb/handle/2016/10398

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kitcha, Suleeporn. “Screening of Oleaginous Yeasts and Optimization for Lipid Production Using Crude Glycerol as a Carbon Source .” 2012. Thesis, Prince of Songkla University. Accessed January 20, 2021. http://kb.psu.ac.th/psukb/handle/2016/10398.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kitcha, Suleeporn. “Screening of Oleaginous Yeasts and Optimization for Lipid Production Using Crude Glycerol as a Carbon Source .” 2012. Web. 20 Jan 2021.

Vancouver:

Kitcha S. Screening of Oleaginous Yeasts and Optimization for Lipid Production Using Crude Glycerol as a Carbon Source . [Internet] [Thesis]. Prince of Songkla University; 2012. [cited 2021 Jan 20]. Available from: http://kb.psu.ac.th/psukb/handle/2016/10398.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kitcha S. Screening of Oleaginous Yeasts and Optimization for Lipid Production Using Crude Glycerol as a Carbon Source . [Thesis]. Prince of Songkla University; 2012. Available from: http://kb.psu.ac.th/psukb/handle/2016/10398

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Rhodes University

22. Johannsen, Elz̀bieta. Hybridization studies within the genus Kluyveromyces van der Walt emend. van der Walt.

Degree: Faculty of Science, Biochemistry and Microbiology, 1979, Rhodes University

URL: http://hdl.handle.net/10962/d1013400

► Hybridization studies based on the prototrophic selection technique, involving the use of auxotrophic mutants of strains of all accepted species of the genus Kluyveromyces, are… (more)

Subjects/Keywords: Yeast fungi – Biotechnology; Yeast fungi – Genetics; Yeast fungi – Hybridization

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APA (6^th Edition):

Johannsen, E. (1979). Hybridization studies within the genus Kluyveromyces van der Walt emend. van der Walt. (Thesis). Rhodes University. Retrieved from http://hdl.handle.net/10962/d1013400

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Johannsen, Elz̀bieta. “Hybridization studies within the genus Kluyveromyces van der Walt emend. van der Walt.” 1979. Thesis, Rhodes University. Accessed January 20, 2021. http://hdl.handle.net/10962/d1013400.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Johannsen, Elz̀bieta. “Hybridization studies within the genus Kluyveromyces van der Walt emend. van der Walt.” 1979. Web. 20 Jan 2021.

Vancouver:

Johannsen E. Hybridization studies within the genus Kluyveromyces van der Walt emend. van der Walt. [Internet] [Thesis]. Rhodes University; 1979. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/10962/d1013400.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Johannsen E. Hybridization studies within the genus Kluyveromyces van der Walt emend. van der Walt. [Thesis]. Rhodes University; 1979. Available from: http://hdl.handle.net/10962/d1013400

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

SUNY College at Brockport

23. Burkhart, Allyson. The Effects of KU70 on Mitochondrial Stability in the Saccharomyces cerevisiae.

Degree: MS, Biology, 2016, SUNY College at Brockport

URL: https://digitalcommons.brockport.edu/bio_theses/21

► The purpose of this experiment is to determine the role of KU70, a nuclear gene, in maintaining mitochondrial DNA in the model organism Saccharomyces… (more)

Subjects/Keywords: KU70 mitochondrial stability yeast genetics; Biology; Genetics and Genomics; Life Sciences; Molecular Genetics

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APA (6^th Edition):

Burkhart, A. (2016). The Effects of KU70 on Mitochondrial Stability in the Saccharomyces cerevisiae. (Thesis). SUNY College at Brockport. Retrieved from https://digitalcommons.brockport.edu/bio_theses/21

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Burkhart, Allyson. “The Effects of KU70 on Mitochondrial Stability in the Saccharomyces cerevisiae.” 2016. Thesis, SUNY College at Brockport. Accessed January 20, 2021. https://digitalcommons.brockport.edu/bio_theses/21.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Burkhart, Allyson. “The Effects of KU70 on Mitochondrial Stability in the Saccharomyces cerevisiae.” 2016. Web. 20 Jan 2021.

Vancouver:

Burkhart A. The Effects of KU70 on Mitochondrial Stability in the Saccharomyces cerevisiae. [Internet] [Thesis]. SUNY College at Brockport; 2016. [cited 2021 Jan 20]. Available from: https://digitalcommons.brockport.edu/bio_theses/21.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Burkhart A. The Effects of KU70 on Mitochondrial Stability in the Saccharomyces cerevisiae. [Thesis]. SUNY College at Brockport; 2016. Available from: https://digitalcommons.brockport.edu/bio_theses/21

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Michigan State University

24. Zhao, Dongyan. Plant mutator-like transposable elements (MULEs) : their evolutionary dynamics, interaction with genes, and recapitulation of transposition activity in yeast.

Degree: 2014, Michigan State University

URL: http://etd.lib.msu.edu/islandora/object/etd:2367

►

Thesis Ph. D. Michigan State University. Plant Breeding, Genetics and Biotechnology - Horticulture - Doctor of Philosophy 2014.

Transposable elements (TEs) are genomic sequences that… (more)

▼

Thesis Ph. D. Michigan State University. Plant Breeding, Genetics and Biotechnology - Horticulture - Doctor of Philosophy 2014.

Transposable elements (TEs) are genomic sequences that can move from one position to another within a genome, where the movement is catalyzed by transposases. Mutator-like transposable elements (MULEs) are a highly mutagenic TE superfamily, which is widespread in a range of organisms. This dissertation focuses on studying the evolution and biology of MULEs in plants, specifically 1) Mechanisms underlying differential abundance of MULEs in maize and rice; 2) Transposition of a rice MULE in yeast; 3) Insertions of MULEs and their impact on gene expression in the Illinois Long-Term Selection Maize population.While the maize genome (2,500 Mb) is six times larger than the rice genome (380 Mb), it contains fewer MULEs than the latter (12,900 vs. 32,000). The differential amplification of MULEs in the two genomes prompted us to investigate the status of MULEs containing transposase (coding-MULEs). Examination of the two genomes indicates that they harbor similar amount of candidate coding-MULEs; however, the majority of candidate coding-MULEs in maize are defective. This is partly due to the higher amount of LTR retrotransposons that disrupt the coding region of MULEs in maize. Additionally, the candidate coding-MULEs seem to be subjected to higher indel rate in maize than that in rice, which accelerate the deterioration of maize elements. Collectively, nested insertions and accumulation of indels may explain the low abundance of MULEs in maize than that in rice. To date, MULEs have been only shown to be active in their native hosts. In this study, transposition of a rice MULE was recapitulated in yeast, representing the first report of MULE transposition in a heterologous species. The wild-type transposase induced low transposition frequency, however, it could be improved by a variety of transposase modifications, including deletion, substitution, and fusion of an enhanced yellow fluorescent protein (EYFP). Deletion of the N-terminal 129 amino acids led to enhanced transposition frequency as well as altered cellular localization of the transposase. Mutational analysis revealed a critical region of the transposase, where changes of the amino acid compositions resulted in either enhanced or repressed activity. Additionally, fusion of an EYFP to the N-terminal deleted transposase also enhanced transposition frequency, which is the first report of transposition activity enhancement by protein fusion. Taken together, the establishment of the MULE transposition system in yeast laid the foundation for further studying MULE biology.MULEs have the propensity to insert into genic regions, which influence the expression of adjacent genes as well as the relevant developmental processes. To determine whether MULEs played any role in the Illinois Long-Term Selection Experiment (ILTSE) maize strains, MULE insertions that are co-segregating with either high or low protein maize strains were…

Advisors/Committee Members: Jiang, Ning, Barry, Cornellius, Grumet, Rebecca, Shiu, Shin-Han, Wang, Dechun.

Subjects/Keywords: Transposons; Yeast – Genetics; Corn – Genetics; Rice – Genetics; Molecular biology; Plant sciences; Bioinformatics

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APA (6^th Edition):

Zhao, D. (2014). Plant mutator-like transposable elements (MULEs) : their evolutionary dynamics, interaction with genes, and recapitulation of transposition activity in yeast. (Thesis). Michigan State University. Retrieved from http://etd.lib.msu.edu/islandora/object/etd:2367

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Zhao, Dongyan. “Plant mutator-like transposable elements (MULEs) : their evolutionary dynamics, interaction with genes, and recapitulation of transposition activity in yeast.” 2014. Thesis, Michigan State University. Accessed January 20, 2021. http://etd.lib.msu.edu/islandora/object/etd:2367.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Zhao, Dongyan. “Plant mutator-like transposable elements (MULEs) : their evolutionary dynamics, interaction with genes, and recapitulation of transposition activity in yeast.” 2014. Web. 20 Jan 2021.

Vancouver:

Zhao D. Plant mutator-like transposable elements (MULEs) : their evolutionary dynamics, interaction with genes, and recapitulation of transposition activity in yeast. [Internet] [Thesis]. Michigan State University; 2014. [cited 2021 Jan 20]. Available from: http://etd.lib.msu.edu/islandora/object/etd:2367.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Zhao D. Plant mutator-like transposable elements (MULEs) : their evolutionary dynamics, interaction with genes, and recapitulation of transposition activity in yeast. [Thesis]. Michigan State University; 2014. Available from: http://etd.lib.msu.edu/islandora/object/etd:2367

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

25. Lee, Hana. Investigating genetic determinants of phenotypic variation in natural isolates of Saccharomyces cerevisiae.

Degree: Molecular & Cell Biology, 2012, University of California – Berkeley

URL: http://www.escholarship.org/uc/item/90c5h46g

► The causal link between genotype and phenotype is one of the fundamental principles of modern biology; yet there remain significant challenges to successfully identifying and… (more)

Subjects/Keywords: Genetics; genomics; quantitative genetics; yeast

…computational approaches. In this respect, the budding yeast, Saccharomyces cerevisiae, has two main… …are applicable to other species. While yeast has long been one of the most well-studied… …to recognize the advantages of studying natural isolates of yeast. In addition to the… …environments make yeast an attractive model not only for addressing questions in quantitative… …genetics but for understanding the genetics of local adaptation (Landry et al., 2006a…

10 more images…

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APA (6^th Edition):

Lee, H. (2012). Investigating genetic determinants of phenotypic variation in natural isolates of Saccharomyces cerevisiae. (Thesis). University of California – Berkeley. Retrieved from http://www.escholarship.org/uc/item/90c5h46g

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lee, Hana. “Investigating genetic determinants of phenotypic variation in natural isolates of Saccharomyces cerevisiae.” 2012. Thesis, University of California – Berkeley. Accessed January 20, 2021. http://www.escholarship.org/uc/item/90c5h46g.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lee, Hana. “Investigating genetic determinants of phenotypic variation in natural isolates of Saccharomyces cerevisiae.” 2012. Web. 20 Jan 2021.

Vancouver:

Lee H. Investigating genetic determinants of phenotypic variation in natural isolates of Saccharomyces cerevisiae. [Internet] [Thesis]. University of California – Berkeley; 2012. [cited 2021 Jan 20]. Available from: http://www.escholarship.org/uc/item/90c5h46g.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lee H. Investigating genetic determinants of phenotypic variation in natural isolates of Saccharomyces cerevisiae. [Thesis]. University of California – Berkeley; 2012. Available from: http://www.escholarship.org/uc/item/90c5h46g

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Durban University of Technology

26. Wakelin, Kyle. Overexpression and partial characterization of a modified fungal xylanase in Escherichia coli.

Degree: 2009, Durban University of Technology

URL: http://hdl.handle.net/10321/480

►

Submitted in complete fulfillment for the Degree of Master of Technology (Biotechnology)in the Department of Biotechnology and Food Technology, Faculty of Applied Sciences, Durban University… (more)

Subjects/Keywords: Biotechnology; Xylanases – Genetics; Escherichia coli – Genetics; Protein engineering; Enzymes – Industrial applications; Yeast fungi – Genetic engineering

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APA (6^th Edition):

Wakelin, K. (2009). Overexpression and partial characterization of a modified fungal xylanase in Escherichia coli. (Thesis). Durban University of Technology. Retrieved from http://hdl.handle.net/10321/480

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Wakelin, Kyle. “Overexpression and partial characterization of a modified fungal xylanase in Escherichia coli.” 2009. Thesis, Durban University of Technology. Accessed January 20, 2021. http://hdl.handle.net/10321/480.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Wakelin, Kyle. “Overexpression and partial characterization of a modified fungal xylanase in Escherichia coli.” 2009. Web. 20 Jan 2021.

Vancouver:

Wakelin K. Overexpression and partial characterization of a modified fungal xylanase in Escherichia coli. [Internet] [Thesis]. Durban University of Technology; 2009. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/10321/480.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Wakelin K. Overexpression and partial characterization of a modified fungal xylanase in Escherichia coli. [Thesis]. Durban University of Technology; 2009. Available from: http://hdl.handle.net/10321/480

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of California – San Francisco

27. Charles, Georgette Mona. Regulation of the SWI/SNF Chromatin Remodeling Complexes by the Non-Catalytic Subunits.

Degree: Biochemistry and Molecular Biology, 2010, University of California – San Francisco

URL: http://www.escholarship.org/uc/item/7310t7m3

► The primary unit of eukaryotic DNA packaging is a nucleosome, which contains ~150 bp of DNA wrapped around an octamer of histone proteins. This packaging… (more)

▼ The primary unit of eukaryotic DNA packaging is a nucleosome, which contains ~150 bp of DNA wrapped around an octamer of histone proteins. This packaging restricts accessibility for transcription, replication, repair, and recombination. ATP-dependent chromatin remodeling complexes play a key role in regulating this accessibility. The catalytic subunits of these complexes use ATP, to alter histone-DNA and histone-histone interactions. Interestingly, the ATPase subunits alone carry out most of the remodeling activities of the entire complexes. This raises the question, of what are the functions of the non-catalytic subunits. Two hypotheses are: (i) non-catalytic subunits enhance the biochemical activity of the catalytic subunit, and (ii) non-catalytic subunits facilitate targeted localization.In Chapter 2, mechanistic roles of the non-catalytic subunits were elucidated. Comparing the hSWI/SNF complex with Brg1, its ATPase subunit, revealed that the non-catalytic subunits do not simply promote an active conformation of Brg1, but rather, enhance discrete steps in the remodeling reaction. Minimal complexes containing, Baf155, Baf170, and Ini1 were sufficient to lower the KM for nucleosomes, suggesting that these non-catalytic subunits help stabilize nucleosome binding. Overall, the data reveal that non-catalytic subunits have important mechanistic roles, including distinguishing between nucleotide states. In Chapter 3, other roles of non-catalytic subunits were identified, following a novel acetylation on the RSC complex, the yeast homolog of hSWI/SNF. Rsc4, the acetylated subunit, contains two bromodomains (BDs). BD1 has been previously shown to bind the acetylation mark when BD2 is not bound to acetylated H3K14 tails. However, we find that acetylation of Rsc4 does not modulate RSC's ability to recognize H3-acetylated nucleosomes. Instead, we found that high Rsc4 acetylation levels as maintained by BD1, are critical for resistance to DNA damage, especially in the absence of another remodeler, INO80. Further, cells lacking Rsc4 acetylation and NHP10, a unique INO80 subunit, have delayed S-phase progression and HU sensitivity, revealing a role for the RSC complex and its acetylation in resistance to replication stress. This study suggests that non-catalytic subunits can specify remodeler participation in cellular processes, distinct from enzymatic functions.

Subjects/Keywords: Chemistry, Biochemistry; Biology, Genetics; Biology, Molecular; Chromatin; Enzymology; Protein Biochemistry; Yeast Genetics

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APA (6^th Edition):

Charles, G. M. (2010). Regulation of the SWI/SNF Chromatin Remodeling Complexes by the Non-Catalytic Subunits. (Thesis). University of California – San Francisco. Retrieved from http://www.escholarship.org/uc/item/7310t7m3

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Charles, Georgette Mona. “Regulation of the SWI/SNF Chromatin Remodeling Complexes by the Non-Catalytic Subunits.” 2010. Thesis, University of California – San Francisco. Accessed January 20, 2021. http://www.escholarship.org/uc/item/7310t7m3.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Charles, Georgette Mona. “Regulation of the SWI/SNF Chromatin Remodeling Complexes by the Non-Catalytic Subunits.” 2010. Web. 20 Jan 2021.

Vancouver:

Charles GM. Regulation of the SWI/SNF Chromatin Remodeling Complexes by the Non-Catalytic Subunits. [Internet] [Thesis]. University of California – San Francisco; 2010. [cited 2021 Jan 20]. Available from: http://www.escholarship.org/uc/item/7310t7m3.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Charles GM. Regulation of the SWI/SNF Chromatin Remodeling Complexes by the Non-Catalytic Subunits. [Thesis]. University of California – San Francisco; 2010. Available from: http://www.escholarship.org/uc/item/7310t7m3

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of California – San Francisco

28. Kliegman, Joseph Ian Morningstar. Chemical-Genetic Characterization of TORC2 in Yeast.

Degree: Biophysics, 2013, University of California – San Francisco

URL: http://www.escholarship.org/uc/item/3mb5352x

► As the clinical focus of cancer therapeutics shift from systemic toxins to targeted therapy, the ability to systematically identify promising candidates for multi-targeted cancer therapy… (more)

Subjects/Keywords: Biophysics; analog-sensitive; chemical genetics; E-MAP; kinase; selective pharmacology; yeast genetics

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APA (6^th Edition):

Kliegman, J. I. M. (2013). Chemical-Genetic Characterization of TORC2 in Yeast. (Thesis). University of California – San Francisco. Retrieved from http://www.escholarship.org/uc/item/3mb5352x

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kliegman, Joseph Ian Morningstar. “Chemical-Genetic Characterization of TORC2 in Yeast.” 2013. Thesis, University of California – San Francisco. Accessed January 20, 2021. http://www.escholarship.org/uc/item/3mb5352x.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kliegman, Joseph Ian Morningstar. “Chemical-Genetic Characterization of TORC2 in Yeast.” 2013. Web. 20 Jan 2021.

Vancouver:

Kliegman JIM. Chemical-Genetic Characterization of TORC2 in Yeast. [Internet] [Thesis]. University of California – San Francisco; 2013. [cited 2021 Jan 20]. Available from: http://www.escholarship.org/uc/item/3mb5352x.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kliegman JIM. Chemical-Genetic Characterization of TORC2 in Yeast. [Thesis]. University of California – San Francisco; 2013. Available from: http://www.escholarship.org/uc/item/3mb5352x

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Washington

29. Clark, Anne Elizabeth. Causes and consequences of genetic variation in budding yeast.

Degree: PhD, 2019, University of Washington

URL: http://hdl.handle.net/1773/44822

► The complex evolutionary forces that shape genomes result in sequences that are part utility and part history. The randomness involved in this process makes it… (more)

▼ The complex evolutionary forces that shape genomes result in sequences that are part utility and part history. The randomness involved in this process makes it difficult to imagine ever being able to read a genome and completely understand the effect of every base. But with large–scale mapping approaches, we can relate specific genetic variants present in natural populations to specific differences in traits of interest. The budding yeast Saccharomyces cerevisiae is one of the most useful model organisms in the study of genetics, and also has a long history of association with human activity. In this dissertation, I explore several methodological aspects related both to mapping traits in S. cerevisiae and to understanding how specific regions of budding yeast genomes have evolved over time. I begin by discussing the two main approaches to positionally mapping trait variation: association and linkage. Both of these approaches are aided by the recent sequencing of hundreds of S. cerevisiae isolates, along with the less extensive but complementary sequencing of other members of the Saccharomyces genus. Genome–wide association is a simple way of taking advantage of the extensive diversity of available sequences, but suffers from inflated false positive rates due to population structure. Linkage mapping has traditionally been more widely applied in yeast, since it is relatively simple to generate very large pools of individuals that are ideal for mapping. This approach remains powerful, but the great number of sequences now available makes it appealing to create larger crosses that incorporate more of this diversity. However, simply mapping trait variation to specific loci does not directly inform us about the mechanisms by which genetic variants affect phenotypes, or about how this variation has arrived at its current state. I also discuss some specific evolutionary aspects of budding yeast genomes. In particular, the ability of these substantially diverged yeasts to hybridize creates interesting possibilities for adaptation. I describe the development and application of an approach for identifying introgressed regions that remain from past hybridization events, and that therefore may have played significant evolutionary roles. Overall, the extensive genetic and phenotypic variation uncovered in budding yeast opens up exciting opportunities to discover the genetic basis of complex traits, and to investigate the larger picture of how evolutionary forces have shaped and continue to shape these experimentally and economically important organisms. Advisors/Committee Members: Akey, Joshua (advisor).

Subjects/Keywords: budding yeast; genomics; introgression; quantitative traits; Saccharomyces; S. cerevisiae; Genetics; Bioinformatics; Biostatistics; Genetics

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APA (6^th Edition):

Clark, A. E. (2019). Causes and consequences of genetic variation in budding yeast. (Doctoral Dissertation). University of Washington. Retrieved from http://hdl.handle.net/1773/44822

Chicago Manual of Style (16^th Edition):

Clark, Anne Elizabeth. “Causes and consequences of genetic variation in budding yeast.” 2019. Doctoral Dissertation, University of Washington. Accessed January 20, 2021. http://hdl.handle.net/1773/44822.

MLA Handbook (7^th Edition):

Clark, Anne Elizabeth. “Causes and consequences of genetic variation in budding yeast.” 2019. Web. 20 Jan 2021.

Vancouver:

Clark AE. Causes and consequences of genetic variation in budding yeast. [Internet] [Doctoral dissertation]. University of Washington; 2019. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/1773/44822.

Council of Science Editors:

Clark AE. Causes and consequences of genetic variation in budding yeast. [Doctoral Dissertation]. University of Washington; 2019. Available from: http://hdl.handle.net/1773/44822

University of Washington

30. Kim, Seungsoo. Maps and mechanisms of three-dimensional genome organization.

Degree: PhD, 2019, University of Washington

URL: http://hdl.handle.net/1773/44275

► The three-dimensional organization of the genome inside the nucleus both impacts and is influenced by its functions, including transcription and DNA replication. Recent technological advances,… (more)

Subjects/Keywords: chromosome conformation; genome; homolog pairing; saturation mutagenesis; yeast; Molecular biology; Genetics; Bioinformatics; Genetics

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kim, S. (2019). Maps and mechanisms of three-dimensional genome organization. (Doctoral Dissertation). University of Washington. Retrieved from http://hdl.handle.net/1773/44275

Chicago Manual of Style (16^th Edition):

Kim, Seungsoo. “Maps and mechanisms of three-dimensional genome organization.” 2019. Doctoral Dissertation, University of Washington. Accessed January 20, 2021. http://hdl.handle.net/1773/44275.

MLA Handbook (7^th Edition):

Kim, Seungsoo. “Maps and mechanisms of three-dimensional genome organization.” 2019. Web. 20 Jan 2021.

Vancouver:

Kim S. Maps and mechanisms of three-dimensional genome organization. [Internet] [Doctoral dissertation]. University of Washington; 2019. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/1773/44275.

Council of Science Editors:

Kim S. Maps and mechanisms of three-dimensional genome organization. [Doctoral Dissertation]. University of Washington; 2019. Available from: http://hdl.handle.net/1773/44275

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Open Access Theses and Dissertations