You searched for subject:(Western Blot).
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NSYSU
1.
Jhou, Siou-An.
A regularly spaced and self-revealing protein ladder for Western blot analysis.
Degree: Master, Institute of Biomedical Sciences, 2014, NSYSU
URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0711114-183817 
► Accurate determination of protein molecular weights is important for Western blot analysis(Towbin et al., 1979). Protein marker plays a very important role in the Western…
(more)
▼ Accurate determination of protein molecular weights is important for
Western blot analysis(Towbin et al., 1979). Protein marker plays a very important role in the
Western blot, it is composed of different molecular weight proteins to present bands on SDS PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) and
western blot. According to different molecule weight band of the protein marker, investigators can estimate the molecule weight of protein sample and figure out if the sample is correct or not. In these experiments, investigators usually rely on pre-stained protein marker to analyze the protein sample(Parkinson and Redshaw, 1984) (Tsang et al., 1984). The advantages of the pre-stained protein marker is the band is stained with different color dye which can be clearly observed when we run the SDS PAGE gel.
Advisors/Committee Members: Ming-Hong Tai (chair), Tian-Lu Cheng (committee member), Bing-hong Chen (chair).
Subjects/Keywords: protein marker; western blot
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APA (6th Edition):
Jhou, S. (2014). A regularly spaced and self-revealing protein ladder for Western blot analysis. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0711114-183817
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Jhou, Siou-An. “A regularly spaced and self-revealing protein ladder for Western blot analysis.” 2014. Thesis, NSYSU. Accessed April 13, 2021.
http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0711114-183817.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Jhou, Siou-An. “A regularly spaced and self-revealing protein ladder for Western blot analysis.” 2014. Web. 13 Apr 2021.
Vancouver:
Jhou S. A regularly spaced and self-revealing protein ladder for Western blot analysis. [Internet] [Thesis]. NSYSU; 2014. [cited 2021 Apr 13].
Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0711114-183817.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Jhou S. A regularly spaced and self-revealing protein ladder for Western blot analysis. [Thesis]. NSYSU; 2014. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0711114-183817
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
2.
下原, 修治; シモハラ, シュウジ.
熱ショックタンパク質を標的にした温熱増感薬の開発研究 : ナフトキノン誘導体のガン温熱療法併用薬としての可能性について : ネツショック タンパクシツ オ ヒョウテキ ニシタ オンネツ ゾウカンヤク ノ カイハツ ケンキュウ ナフトキノン ユウドウタイ ノ ガン オンネツ リョウホウ ヘイヨウヤク トシテノ カノウセイ ニツイテ; Development of a hyperthermic sensitizer targeting heat shock proteins : Possibility of napthoquinone derivatives as sensitizers of hyperthermic cancer therapy.
Degree: Kumamoto University / 熊本大学
URL: http://hdl.handle.net/2298/2644
Subjects/Keywords: Western blot
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APA (6th Edition):
下原, 修治; シモハラ, . (n.d.). 熱ショックタンパク質を標的にした温熱増感薬の開発研究 : ナフトキノン誘導体のガン温熱療法併用薬としての可能性について : ネツショック タンパクシツ オ ヒョウテキ ニシタ オンネツ ゾウカンヤク ノ カイハツ ケンキュウ ナフトキノン ユウドウタイ ノ ガン オンネツ リョウホウ ヘイヨウヤク トシテノ カノウセイ ニツイテ; Development of a hyperthermic sensitizer targeting heat shock proteins : Possibility of napthoquinone derivatives as sensitizers of hyperthermic cancer therapy. (Thesis). Kumamoto University / 熊本大学. Retrieved from http://hdl.handle.net/2298/2644
Note: this citation may be lacking information needed for this citation format:
No year of publication.
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
下原, 修治; シモハラ, シュウジ. “熱ショックタンパク質を標的にした温熱増感薬の開発研究 : ナフトキノン誘導体のガン温熱療法併用薬としての可能性について : ネツショック タンパクシツ オ ヒョウテキ ニシタ オンネツ ゾウカンヤク ノ カイハツ ケンキュウ ナフトキノン ユウドウタイ ノ ガン オンネツ リョウホウ ヘイヨウヤク トシテノ カノウセイ ニツイテ; Development of a hyperthermic sensitizer targeting heat shock proteins : Possibility of napthoquinone derivatives as sensitizers of hyperthermic cancer therapy.” Thesis, Kumamoto University / 熊本大学. Accessed April 13, 2021.
http://hdl.handle.net/2298/2644.
Note: this citation may be lacking information needed for this citation format:
No year of publication.
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
下原, 修治; シモハラ, シュウジ. “熱ショックタンパク質を標的にした温熱増感薬の開発研究 : ナフトキノン誘導体のガン温熱療法併用薬としての可能性について : ネツショック タンパクシツ オ ヒョウテキ ニシタ オンネツ ゾウカンヤク ノ カイハツ ケンキュウ ナフトキノン ユウドウタイ ノ ガン オンネツ リョウホウ ヘイヨウヤク トシテノ カノウセイ ニツイテ; Development of a hyperthermic sensitizer targeting heat shock proteins : Possibility of napthoquinone derivatives as sensitizers of hyperthermic cancer therapy.” Web. 13 Apr 2021.
Note: this citation may be lacking information needed for this citation format:
No year of publication.
Vancouver:
下原, 修治; シモハラ . 熱ショックタンパク質を標的にした温熱増感薬の開発研究 : ナフトキノン誘導体のガン温熱療法併用薬としての可能性について : ネツショック タンパクシツ オ ヒョウテキ ニシタ オンネツ ゾウカンヤク ノ カイハツ ケンキュウ ナフトキノン ユウドウタイ ノ ガン オンネツ リョウホウ ヘイヨウヤク トシテノ カノウセイ ニツイテ; Development of a hyperthermic sensitizer targeting heat shock proteins : Possibility of napthoquinone derivatives as sensitizers of hyperthermic cancer therapy. [Internet] [Thesis]. Kumamoto University / 熊本大学; [cited 2021 Apr 13].
Available from: http://hdl.handle.net/2298/2644.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
No year of publication.
Council of Science Editors:
下原, 修治; シモハラ . 熱ショックタンパク質を標的にした温熱増感薬の開発研究 : ナフトキノン誘導体のガン温熱療法併用薬としての可能性について : ネツショック タンパクシツ オ ヒョウテキ ニシタ オンネツ ゾウカンヤク ノ カイハツ ケンキュウ ナフトキノン ユウドウタイ ノ ガン オンネツ リョウホウ ヘイヨウヤク トシテノ カノウセイ ニツイテ; Development of a hyperthermic sensitizer targeting heat shock proteins : Possibility of napthoquinone derivatives as sensitizers of hyperthermic cancer therapy. [Thesis]. Kumamoto University / 熊本大学; Available from: http://hdl.handle.net/2298/2644
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
No year of publication.
University of Debrecen
3.
Vaskó, Barbara.
Az antigén-ellenanyag kapcsolódásán alapuló jelzéses analitikai módszerek: ELISA, Western blot
.
Degree: DE – TEK – Természettudományi és Technológiai Kar – Biológiai és Ökológiai Intézet, 2011, University of Debrecen
URL: http://hdl.handle.net/2437/118359
► Az utóbbi években a mindennapi laboratóriumi diagnosztikában egyre nagyobb teret hódítanak az immunanalitikai módszerek (immunoassay-k), amelyek specificitásukkal, szelektivitásukkal, egyszerűségükkel és gyorsaságukkal méltán válhatnak a kromatográfiás…
(more)
▼ Az utóbbi években a mindennapi laboratóriumi diagnosztikában egyre nagyobb teret hódítanak az immunanalitikai módszerek (immunoassay-k), amelyek specificitásukkal, szelektivitásukkal, egyszerűségükkel és gyorsaságukkal méltán válhatnak a kromatográfiás módszerek vetélytársaivá. Szakdolgozatom bemutatja az immunanalitikai módszerek jelentőségét a laboratóriumi vizsgálatokban, és az antitest-antigén kölcsönhatásán alapuló jelzéses analitikai technikák két legelterjedtebb módszerét: az ELISA és a
Western blot típusait, a kivitelezésük lépéseit, a meghatározáshoz szükséges eszközöket, reagenseket és műszereket, valamint a technikák gyakorlati alkalmazását.
Advisors/Committee Members: Csákiné Jäger, Szilvia (advisor).
Subjects/Keywords: ELISA;
Western blot;
antigén-ellenanyag
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APA (6th Edition):
Vaskó, B. (2011). Az antigén-ellenanyag kapcsolódásán alapuló jelzéses analitikai módszerek: ELISA, Western blot
. (Thesis). University of Debrecen. Retrieved from http://hdl.handle.net/2437/118359
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Vaskó, Barbara. “Az antigén-ellenanyag kapcsolódásán alapuló jelzéses analitikai módszerek: ELISA, Western blot
.” 2011. Thesis, University of Debrecen. Accessed April 13, 2021.
http://hdl.handle.net/2437/118359.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Vaskó, Barbara. “Az antigén-ellenanyag kapcsolódásán alapuló jelzéses analitikai módszerek: ELISA, Western blot
.” 2011. Web. 13 Apr 2021.
Vancouver:
Vaskó B. Az antigén-ellenanyag kapcsolódásán alapuló jelzéses analitikai módszerek: ELISA, Western blot
. [Internet] [Thesis]. University of Debrecen; 2011. [cited 2021 Apr 13].
Available from: http://hdl.handle.net/2437/118359.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Vaskó B. Az antigén-ellenanyag kapcsolódásán alapuló jelzéses analitikai módszerek: ELISA, Western blot
. [Thesis]. University of Debrecen; 2011. Available from: http://hdl.handle.net/2437/118359
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
4.
Pano, Glendin Kostantinos.
Identifying Transcription Co-Regulators of Gcm/Repo in the Development of Glia in Drosophila Melanogaster.
Degree: M.S. in Biological Science, Biology, 2019, University of Mississippi
URL: https://egrove.olemiss.edu/etd/1653
► The molecular mechanism mediating the differentiation of neural progenitors to either glia or neurons is guided by the gene glial cells missing (gcm), a master…
(more)
▼ The molecular mechanism mediating the differentiation of neural progenitors to either glia or neurons is guided by the gene
glial cells missing (gcm), a master regulator of glial cell differentiation. Knockout of this transcription factor (TF) and its homolog
gcm2 in the fruit fly
Drosophila Melanogaster results in embryos that develop neurons in place of lateral glia. Conversely, overexpression of
gcm in those neural progenitors results in embryos that have substantially fewer neurons and excess glia(Jones, et.al, 1995; Hosoya, et.al, 1995; and Vincent, et.al, 1996). Additionally,
gcm has also been shown to be involved in the differentiation of blood cells, tendon cells, and lamina cells, suggesting that it interacts with other transcriptional regulators to exert different influences in different contexts (Jones, 2005; Johnson, et al.,2012). A Yeast One Hybrid screen was used to identify such co-factors (Nipper, 2014). Two protein candidates that showed a strong interaction with Gcm were Groucho (Gro) and the B-prime regulatory subunit of the
widerborst (wdb) gene (Nipper, 2014). In order to verify these interactions and further investigate them, it is necessary to develop a protocol where the genes of these proteins can be co-expressed in S2 cells. The goal of this thesis was to establish a protocol that outlines the proper conditions for
gcm expression in S2 cells, and the subsequent staining of its protein product on a
Western blot. Several steps along this process were optimized to achieve this result. This protocol can now be used in the Jones lab to investigate these protein candidates further. A co-immunoprecipitation of Gcm with the aforementioned protein candidates could confirm interaction. It would be of interest to investigate how the protein candidates affect a key glial gene–
repo–whose regulatory DNA is controlled by Gcm and potentially other transcription factors.
Advisors/Committee Members: Bradley Jones, Mika Jekabsons, Sarah Liljegren.
Subjects/Keywords: Drosophila Melanogaster; Western Blot; Biology
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APA ·
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APA (6th Edition):
Pano, G. K. (2019). Identifying Transcription Co-Regulators of Gcm/Repo in the Development of Glia in Drosophila Melanogaster. (Thesis). University of Mississippi. Retrieved from https://egrove.olemiss.edu/etd/1653
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Pano, Glendin Kostantinos. “Identifying Transcription Co-Regulators of Gcm/Repo in the Development of Glia in Drosophila Melanogaster.” 2019. Thesis, University of Mississippi. Accessed April 13, 2021.
https://egrove.olemiss.edu/etd/1653.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Pano, Glendin Kostantinos. “Identifying Transcription Co-Regulators of Gcm/Repo in the Development of Glia in Drosophila Melanogaster.” 2019. Web. 13 Apr 2021.
Vancouver:
Pano GK. Identifying Transcription Co-Regulators of Gcm/Repo in the Development of Glia in Drosophila Melanogaster. [Internet] [Thesis]. University of Mississippi; 2019. [cited 2021 Apr 13].
Available from: https://egrove.olemiss.edu/etd/1653.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Pano GK. Identifying Transcription Co-Regulators of Gcm/Repo in the Development of Glia in Drosophila Melanogaster. [Thesis]. University of Mississippi; 2019. Available from: https://egrove.olemiss.edu/etd/1653
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Minnesota
5.
Ablorh, Naa-Adjeley Dromoh.
Accurate quantitation of phospholamban expression and phosphorylation in biological samples.
Degree: PhD, Biochemistry, Molecular Bio, and Biophysics, 2013, University of Minnesota
URL: http://purl.umn.edu/155524
► Phospholamban (PLB) reversibly inhibits the sarcoplasmic reticulum calcium ATP-ase (SERCA) in cardiomyocytes. When SERCA is active, it pumps calcium into the sarcoplasmic reticulum (SR) to…
(more)
▼ Phospholamban (PLB) reversibly inhibits the sarcoplasmic reticulum calcium ATP-ase (SERCA) in cardiomyocytes. When SERCA is active, it pumps calcium into the sarcoplasmic reticulum (SR) to reduce cytosolic [Ca++]. Calcium efflux from the cytosol reduces the Ca++ available to the cytosolic contractile apparatus so that the heart can relax during diastole. The extent of relaxation depends on the amount of calcium that SERCA removes from the cytosol during diastole, while the contractile force depends on the magnitude of the end-diastolic calcium transient. Thus SERCA inhibition affects both the contractile and relaxation phases of the cardiac cycle. Unphosphorylated PLB (uPLB) inhibits SERCA at low Ca++ concentration and phosphorylated PLB (pPLB) is less inhibitory, so myocardial physiology and pathology depend critically on the mole fraction of pPLB, Xp, equal to pPLB/(uPLB+pPLB), the concentrations of total PLB (tPLB) and SERCA, and uPLB/SERCA.
Prior to our assay, neither Xp nor tPLB could be measured accurately. Previous measurements relied on radioactive tracers, which only measured changes in these parameters, or immunoblots, which did not provide acceptable precision or accuracy. The fundamental problems with immunoblots were due to the lack of (a) accurate standards for pPLB and uPLB, (b) antibodies completely specific for pPLB and uPLB, and (c) a mathematical relationship between the antibody selectivity, the intensities of the samples and Xp. I have solved these problems using purified uPLB and pPLB standards, produced by solid-phase peptide synthesis, by performing two parallel immunoblots with antibodies partially specific for uPLB and pPLB, and deriving accurate equations for calculating Xp and tPLB. When this method was applied to mixtures of known composition, it measured both Xp and tPLB with ≥ 96% accuracy. I used this assay on samples of pig cardiac SR and found that Xp varied widely among four animals, from 0.08 to 0.38, but there was remarkably little variation in the ratios of Xp/tPLB and uPLB/SERCA, suggesting that PLB phosphorylation is tuned to maintain homeostasis in SERCA regulation. I have extended this method to measure accurately the mole fractions of PLB phosphorylated at Ser16, Thr17 and bisphospho-Ser16-Thr17 in biological samples, and to analyze the PLB phosphorylation status of cardiac tissue samples obtained from human patients with specific cardiomyopathies. This assay can be adapted to any phospho-protein, and with any other posttranslational modification where purified standards and partially specific antibodies are available.
Subjects/Keywords: Phospholamban; Quantitative western blot
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APA ·
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APA (6th Edition):
Ablorh, N. D. (2013). Accurate quantitation of phospholamban expression and phosphorylation in biological samples. (Doctoral Dissertation). University of Minnesota. Retrieved from http://purl.umn.edu/155524
Chicago Manual of Style (16th Edition):
Ablorh, Naa-Adjeley Dromoh. “Accurate quantitation of phospholamban expression and phosphorylation in biological samples.” 2013. Doctoral Dissertation, University of Minnesota. Accessed April 13, 2021.
http://purl.umn.edu/155524.
MLA Handbook (7th Edition):
Ablorh, Naa-Adjeley Dromoh. “Accurate quantitation of phospholamban expression and phosphorylation in biological samples.” 2013. Web. 13 Apr 2021.
Vancouver:
Ablorh ND. Accurate quantitation of phospholamban expression and phosphorylation in biological samples. [Internet] [Doctoral dissertation]. University of Minnesota; 2013. [cited 2021 Apr 13].
Available from: http://purl.umn.edu/155524.
Council of Science Editors:
Ablorh ND. Accurate quantitation of phospholamban expression and phosphorylation in biological samples. [Doctoral Dissertation]. University of Minnesota; 2013. Available from: http://purl.umn.edu/155524
6.
Piarroux, Raphaël.
Développement de tests de diagnostic in vitro appliqués au sérodiagnostic des infections fongiques par western blot et immunochromatographie : Development of in vitro diagnostic test applied to the diagnosis of fungal infections by western blot and immunochromatography.
Degree: Docteur es, Biologie. Microbiologie, 2018, Aix Marseille Université
URL: http://www.theses.fr/2018AIXM0763
► Le champignon microscopique Aspergillus fumigatus provoque un nombre important de maladies graves. Parmi elles, l’aspergillose pulmonaire chronique (APC) et l’aspergillose broncho-pulmonaire allergique (ABPA) affectent 3…
(more)
▼ Le champignon microscopique Aspergillus fumigatus provoque un nombre important de maladies graves. Parmi elles, l’aspergillose pulmonaire chronique (APC) et l’aspergillose broncho-pulmonaire allergique (ABPA) affectent 3 et 4,8 millions de personnes dans le monde, respectivement.L’APC est très souvent mortelle si elle n’est pas soignée. Elle se développe très souvent après une tuberculose. C’est donc une maladie des pays émergents, où il n’est souvent pas possible de la diagnostiquer à cause du coût trop important des techniques existantes.L’ABPA est une complication très grave de l’asthme et de la mucoviscidose, qui complique fortement ces maladies. Elle est très difficile à diagnostiquer.Notre travail a donc consisté à développer et évaluer deux tests, un test rapide permettant de poser le diagnostic d’APC sans avoir à utiliser de matériel de laboratoire à destination des pays émergents et un western blot qui permet la confirmation du diagnostic d’ABPA.
Aspergillus fumigatus is a microscopic fungus that can cause numerous diseases. Among them, chronic pulmonary aspergillosis (CPA) and allergic broncho-pulmonary aspergilloses (ABPA) affect 3 and 4.8 million people, respectively.CPA is often fatal if left untreated. It is often a complication of tuberculosis and therefore affect low and middle income countries. However, it is difficult to diagnose it in those countries, as the tests are too expansive.ABPA is a severe complication of asthma and cystic fibrosis, worsening those diseases. It’s very hard to diagnose it.Our work was to develop and evaluate two tests, a rapid test for the diagnosis of CPA that does not require laboratory equipment designed for low and middle income countries and a western blot for confirmation of ABPA diagnosis.
Advisors/Committee Members: Ranque, Stéphane (thesis director).
Subjects/Keywords: Western blot; Immunochromatographie; Aspergillus; Sérologie; Aspergillus; Western blot; Immunochromatography; Serology
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APA ·
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MLA ·
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CSE |
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Manager
APA (6th Edition):
Piarroux, R. (2018). Développement de tests de diagnostic in vitro appliqués au sérodiagnostic des infections fongiques par western blot et immunochromatographie : Development of in vitro diagnostic test applied to the diagnosis of fungal infections by western blot and immunochromatography. (Doctoral Dissertation). Aix Marseille Université. Retrieved from http://www.theses.fr/2018AIXM0763
Chicago Manual of Style (16th Edition):
Piarroux, Raphaël. “Développement de tests de diagnostic in vitro appliqués au sérodiagnostic des infections fongiques par western blot et immunochromatographie : Development of in vitro diagnostic test applied to the diagnosis of fungal infections by western blot and immunochromatography.” 2018. Doctoral Dissertation, Aix Marseille Université. Accessed April 13, 2021.
http://www.theses.fr/2018AIXM0763.
MLA Handbook (7th Edition):
Piarroux, Raphaël. “Développement de tests de diagnostic in vitro appliqués au sérodiagnostic des infections fongiques par western blot et immunochromatographie : Development of in vitro diagnostic test applied to the diagnosis of fungal infections by western blot and immunochromatography.” 2018. Web. 13 Apr 2021.
Vancouver:
Piarroux R. Développement de tests de diagnostic in vitro appliqués au sérodiagnostic des infections fongiques par western blot et immunochromatographie : Development of in vitro diagnostic test applied to the diagnosis of fungal infections by western blot and immunochromatography. [Internet] [Doctoral dissertation]. Aix Marseille Université 2018. [cited 2021 Apr 13].
Available from: http://www.theses.fr/2018AIXM0763.
Council of Science Editors:
Piarroux R. Développement de tests de diagnostic in vitro appliqués au sérodiagnostic des infections fongiques par western blot et immunochromatographie : Development of in vitro diagnostic test applied to the diagnosis of fungal infections by western blot and immunochromatography. [Doctoral Dissertation]. Aix Marseille Université 2018. Available from: http://www.theses.fr/2018AIXM0763
Universidade Estadual de Campinas
7.
Gonzalez-Arriagada, Wilfredo Alejandro, 1980-.
Expression of SPLUNC1 (BPIFA1) and SPLUNC2A (BPIFA2A) in saliva of patients undergoing radiotherapy : Expressão de SPLUNC1 (BPIFA1) e SPLUNC2A (BPIFA2A) na saliva de pacientes submetidos à radioterapia.
Degree: Faculdade de Odontologia de Piracicaba; Programa de Pós-Graduação em Estomatopatologia, 2014, Universidade Estadual de Campinas
URL: GONZALEZ-ARRIAGADA,
Wilfredo
Alejandro.
Expression
of
SPLUNC1
(BPIFA1)
and
SPLUNC2A
(BPIFA2A)
in
saliva
of
patients
undergoing
radiotherapy:
Expressão
de
SPLUNC1
(BPIFA1)
e
SPLUNC2A
(BPIFA2A)
na
saliva
de
pacientes
submetidos
à
radioterapia.
2014.
57
f.
Tese
(doutorado)
-
Universidade
Estadual
de
Campinas,
Faculdade
de
Odontologia
de
Piracicaba,
Piracicaba,
SP.
Disponível
em:
<http://www.repositorio.unicamp.br/handle/REPOSIP/287862>.
Acesso
em:
6
fev.
2019.
;
http://repositorio.unicamp.br/jspui/handle/REPOSIP/287862
► Orientador: Márcio Ajudarte Lopes
Tese (doutorado) - Universidade Estadual de Campinas, Faculdade de Odontologia de Piracicaba
Made available in DSpace on 2019-02-06T15:56:13Z (GMT). No. of…
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▼ Orientador: Márcio Ajudarte Lopes
Tese (doutorado) - Universidade Estadual de Campinas, Faculdade de Odontologia de Piracicaba
Made available in DSpace on 2019-02-06T15:56:13Z (GMT). No. of bitstreams: 1 Gonzalez-Arriagada_WilfredoAlejandro_D.pdf: 1774961 bytes, checksum: b24f3765bd65d083c27d09fc2ffbd39b (MD5) Previous issue date: 2014
Resumo: A radioterapia causa alterações na composição salivar e as proteínas PLUNC participam na resposta imune inata da cavidade oral. O objetivo desse estudo foi verificar se a radioterapia é capaz de modificar a expressão de PLUNC salivar e se essas proteínas estão associadas com os efeitos colaterais. MATERIAIS E MÉTODOS: Foi coletada saliva não estimulada de 65 voluntários (45 pacientes com câncer e 20 controles). No grupo de estudo a coleta foi realizada uma semana antes do início da radioterapia, no meio do tratamento e uma semana após o término. A expressão de SPLUNC1 e SPLUNC2A foi detectada por
western blot, e foi analisada com os dados clínico-patológicos e efeitos colaterais. RESULTADOS: Foi notada uma redução do fluxo salivar durante e após o término da radioterapia, sendo mais acentuada nos pacientes que foram submetidos a radioterapia envolvendo a região facial. O campo de radiação facial foi correlacionado com os efeitos colaterais, principalmente com a presença (p=0,0110) e intensidade (p=0,0143) de mucosite. SPLUNC1 e SPLUNC2A foram detectadas na saliva dos pacientes sem tratamento em concentrações variáveis. O grupo de estudo mostrou níveis de SPLUNC2A significantemente maiores que o grupo controle, enquanto SPLUNC1 não mostrou diferenças. A concentração de PLUNC foi modificada pela radioterapia, observando diminuição dos níveis de SPLUNC2A na sua forma glicosilada (p<,0001) e aumento dos níveis de SPLUNC1 (p=0,0081) na segunda e terceira coletas. A única associação entre efeitos colaterais da radioterapia e PLUNC foi a presença (p=0,0363) e intensidade
(p=0,0500) da mucosite com maiores níveis SPLUNC1. CONCLUSÕES: O presente estudo reportou que os níveis de SPLUNC1 e SPLUNC2A glicosilada são afetados pela radioterapia, sugerindo que essas proteínas podem ter importância no microambiente oral dos pacientes irradiados na cabeça e pescoço
Radiotherapy causes alteration in saliva composition and PLUNC proteins participate in innate immunity of the oral cavity. The aim of this study was to verify if radiotherapy is able to modify the salivary PLUNC expression and if they are associated with radiotherapy side-effects. MATERIALS AND METHODS: Unstimulated whole-mouth saliva of 65 voluntaries (45 cancer patients and 20 controls) was collected. In the study group the collection was performed one week before the beginning of radiotherapy, in the middle of the treatment and one week after finishing. SPLUNC1 and SPLUNC2A expression were detected by western blotting and was analyzed with clinicopathological data and radiotherapy side-effects.
RESULTS: Reduction of salivary flow rates was observed during and after conclusion of radiotherapy, being more accentuated in…
Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS, Lopes, Márcio Ajudarte, 1967-, Alves, Fabio de Abreu, Bufalino, Andreia, Vargas, Pablo Agustin, Silva, Andreia Aparecida da.
Subjects/Keywords: Western blot; Mucosite; Candidíase; Western blot; Mucositis; Candidiasis
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APA (6th Edition):
Gonzalez-Arriagada, Wilfredo Alejandro, 1. (2014). Expression of SPLUNC1 (BPIFA1) and SPLUNC2A (BPIFA2A) in saliva of patients undergoing radiotherapy : Expressão de SPLUNC1 (BPIFA1) e SPLUNC2A (BPIFA2A) na saliva de pacientes submetidos à radioterapia. (Doctoral Dissertation). Universidade Estadual de Campinas. Retrieved from GONZALEZ-ARRIAGADA, Wilfredo Alejandro. Expression of SPLUNC1 (BPIFA1) and SPLUNC2A (BPIFA2A) in saliva of patients undergoing radiotherapy: Expressão de SPLUNC1 (BPIFA1) e SPLUNC2A (BPIFA2A) na saliva de pacientes submetidos à radioterapia. 2014. 57 f. Tese (doutorado) - Universidade Estadual de Campinas, Faculdade de Odontologia de Piracicaba, Piracicaba, SP. Disponível em: <http://www.repositorio.unicamp.br/handle/REPOSIP/287862>. Acesso em: 6 fev. 2019. ; http://repositorio.unicamp.br/jspui/handle/REPOSIP/287862
Chicago Manual of Style (16th Edition):
Gonzalez-Arriagada, Wilfredo Alejandro, 1980-. “Expression of SPLUNC1 (BPIFA1) and SPLUNC2A (BPIFA2A) in saliva of patients undergoing radiotherapy : Expressão de SPLUNC1 (BPIFA1) e SPLUNC2A (BPIFA2A) na saliva de pacientes submetidos à radioterapia.” 2014. Doctoral Dissertation, Universidade Estadual de Campinas. Accessed April 13, 2021.
GONZALEZ-ARRIAGADA, Wilfredo Alejandro. Expression of SPLUNC1 (BPIFA1) and SPLUNC2A (BPIFA2A) in saliva of patients undergoing radiotherapy: Expressão de SPLUNC1 (BPIFA1) e SPLUNC2A (BPIFA2A) na saliva de pacientes submetidos à radioterapia. 2014. 57 f. Tese (doutorado) - Universidade Estadual de Campinas, Faculdade de Odontologia de Piracicaba, Piracicaba, SP. Disponível em: <http://www.repositorio.unicamp.br/handle/REPOSIP/287862>. Acesso em: 6 fev. 2019. ; http://repositorio.unicamp.br/jspui/handle/REPOSIP/287862.
MLA Handbook (7th Edition):
Gonzalez-Arriagada, Wilfredo Alejandro, 1980-. “Expression of SPLUNC1 (BPIFA1) and SPLUNC2A (BPIFA2A) in saliva of patients undergoing radiotherapy : Expressão de SPLUNC1 (BPIFA1) e SPLUNC2A (BPIFA2A) na saliva de pacientes submetidos à radioterapia.” 2014. Web. 13 Apr 2021.
Vancouver:
Gonzalez-Arriagada, Wilfredo Alejandro 1. Expression of SPLUNC1 (BPIFA1) and SPLUNC2A (BPIFA2A) in saliva of patients undergoing radiotherapy : Expressão de SPLUNC1 (BPIFA1) e SPLUNC2A (BPIFA2A) na saliva de pacientes submetidos à radioterapia. [Internet] [Doctoral dissertation]. Universidade Estadual de Campinas; 2014. [cited 2021 Apr 13].
Available from: GONZALEZ-ARRIAGADA, Wilfredo Alejandro. Expression of SPLUNC1 (BPIFA1) and SPLUNC2A (BPIFA2A) in saliva of patients undergoing radiotherapy: Expressão de SPLUNC1 (BPIFA1) e SPLUNC2A (BPIFA2A) na saliva de pacientes submetidos à radioterapia. 2014. 57 f. Tese (doutorado) - Universidade Estadual de Campinas, Faculdade de Odontologia de Piracicaba, Piracicaba, SP. Disponível em: <http://www.repositorio.unicamp.br/handle/REPOSIP/287862>. Acesso em: 6 fev. 2019. ; http://repositorio.unicamp.br/jspui/handle/REPOSIP/287862.
Council of Science Editors:
Gonzalez-Arriagada, Wilfredo Alejandro 1. Expression of SPLUNC1 (BPIFA1) and SPLUNC2A (BPIFA2A) in saliva of patients undergoing radiotherapy : Expressão de SPLUNC1 (BPIFA1) e SPLUNC2A (BPIFA2A) na saliva de pacientes submetidos à radioterapia. [Doctoral Dissertation]. Universidade Estadual de Campinas; 2014. Available from: GONZALEZ-ARRIAGADA, Wilfredo Alejandro. Expression of SPLUNC1 (BPIFA1) and SPLUNC2A (BPIFA2A) in saliva of patients undergoing radiotherapy: Expressão de SPLUNC1 (BPIFA1) e SPLUNC2A (BPIFA2A) na saliva de pacientes submetidos à radioterapia. 2014. 57 f. Tese (doutorado) - Universidade Estadual de Campinas, Faculdade de Odontologia de Piracicaba, Piracicaba, SP. Disponível em: <http://www.repositorio.unicamp.br/handle/REPOSIP/287862>. Acesso em: 6 fev. 2019. ; http://repositorio.unicamp.br/jspui/handle/REPOSIP/287862
University of Michigan
8.
Jin, Shi.
Development of Microfluidic Based Western Blot Technology for Fast and High-Content Analyses.
Degree: PhD, Chemistry, 2015, University of Michigan
URL: http://hdl.handle.net/2027.42/116663
► Western blotting has been a highly valued method in research for protein identification and relative quantitation in complex biological samples. Although widely adapted, Western blot…
(more)
▼ Western blotting has been a highly valued method in research for protein identification and relative quantitation in complex biological samples. Although widely adapted,
Western blot assays have many limitations including labor intensive, low throughput, low information content, large sample consumption, and mediocre repeatability.
A microfluidic
Western blotting system based on protein sieving on microchips followed by direct blotting onto a moving membrane is described. By flowing same separation sieving buffer by the sides of separation channel, protein bands are well defined and transferred onto membrane with minimum band broadening, which is an improvement to a previous hybrid capillary
Western blotting system. Separation speed is significantly improved on a microchip due to a high electric field applied. A complete
Western blot for actin is finished in about 25 min with 0.7 nM detection limit.
While speed of separation is important to some applications, in many cases resolution between closely related protein isoforms is required. 2 kDa different proteins are used to demonstrate the achievement of improved separation resolution by modifying channel dimensions and lengths. With an 8 cm long separation channel, ERK1 (44 kDa) and ERK2 (42 kDa) are baseline resolved on the membrane within 8 min.
Another advantage of using microfluidic devices for
Western blotting is the potential for high throughput and high content analysis. Up to seven parallel separation channels, each with 3 sample reservoirs, are accommodated on a 3 inch by 3 inch glass chip. 21
Western blots are completed within 30 min. To demonstrate the capability of detecting multiple proteins using a small amount of cell lysate sample, 11 proteins are detected using less than 200 nL lysate sample (0.4 μg total protein). For comparison, traditional assays would require at least 90 μL (90 μg total protein) to finish the same task.
Overall, microfluidic based
Western blotting technology has shown promise for fast analysis, low sample consumption, and multiplexing over traditional methods.
Advisors/Committee Members: Kennedy, Robert T. (committee member), Burns, Mark A (committee member), Hakansson, Kristina I. (committee member), Mapp, Anna K (committee member).
Subjects/Keywords: Microfluidic based Western blot; Chemistry; Science
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APA (6th Edition):
Jin, S. (2015). Development of Microfluidic Based Western Blot Technology for Fast and High-Content Analyses. (Doctoral Dissertation). University of Michigan. Retrieved from http://hdl.handle.net/2027.42/116663
Chicago Manual of Style (16th Edition):
Jin, Shi. “Development of Microfluidic Based Western Blot Technology for Fast and High-Content Analyses.” 2015. Doctoral Dissertation, University of Michigan. Accessed April 13, 2021.
http://hdl.handle.net/2027.42/116663.
MLA Handbook (7th Edition):
Jin, Shi. “Development of Microfluidic Based Western Blot Technology for Fast and High-Content Analyses.” 2015. Web. 13 Apr 2021.
Vancouver:
Jin S. Development of Microfluidic Based Western Blot Technology for Fast and High-Content Analyses. [Internet] [Doctoral dissertation]. University of Michigan; 2015. [cited 2021 Apr 13].
Available from: http://hdl.handle.net/2027.42/116663.
Council of Science Editors:
Jin S. Development of Microfluidic Based Western Blot Technology for Fast and High-Content Analyses. [Doctoral Dissertation]. University of Michigan; 2015. Available from: http://hdl.handle.net/2027.42/116663
9.
Sena, Glauber Santos Ribeiro de.
Ocorr??ncia da infec????o por lentivirus de pequenos ruminantes em ovinos e caprinos do semi??rido baiano e caracter??sticas deste sistema produtivo na regi??o.
Degree: 2013, Brazil
URL: http://www.repositorio.ufba.br/ri/handle/ri/12190
► Submitted by Hiolanda R??go ([email protected]) on 2013-07-15T20:04:59Z No. of bitstreams: 1 Disserta????o_ICS_Glauber Santos Ribeiro de Sena.pdf: 1217162 bytes, checksum: f7bea02af0d0becfe7772cc677cf106d (MD5)
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▼ Submitted by Hiolanda R??go ([email protected]) on 2013-07-15T20:04:59Z No. of bitstreams: 1 Disserta????o_ICS_Glauber Santos Ribeiro de Sena.pdf: 1217162 bytes, checksum: f7bea02af0d0becfe7772cc677cf106d (MD5)
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CAPES; FAPESB
Este trabalho teve como objetivo avaliar a presen??a dos LVPR em propriedades dos territ??rios de identidade baianos do Portal do Sert??o, Sisal e Bacia do Jacu??pe e caracterizar o perfil socioecon??mico da caprino/ovinocultura nestas regi??es. Em 134 propriedades foram coletados soros caprinos (n=1046) e ovinos (n = 704). Estes soros foram submetidos aos testes de IDGA, ELISA e Western Blot. Durante a coleta, aplicou-se um question??rio para definir o perfil socioecon??mico da caprino/ovinocultura dessas regi??es. Os
resultados desta pesquisa mostraram a aus??ncia de animais positivos para CAEV atrav??s do IDGA, mas 10 animais positivos pelo ELISA e Western blot. N??o foram encontrados ovinos positivos para Maedi-Visna pelo IDGA. A an??lise do question??rio aplicado nas propriedades mostrou um perfil produtivo pouco tecnificado, com predom??nio de pequenas propriedades com gest??o familiar. Os rebanhos s??o pequenos, criados em sistema extensivo e formados principalmente por animais sem ra??a definida. Observou-se pouco investimento em pr??ticas e instala????es adequadas para o manejo alimentar, sanit??rio, reprodutivo e das crias que possibilitem o aumento da produtividade nas propriedades. A pequena produ????o tem como finalidade o corte (carne) e se destina a subsist??ncia familiar e ao com??rcio local. Discute-se os alcances do desenvolvimento da caprino/ovinocultura na regi??o.
Salvador
Advisors/Committee Members: Sardi, Silvia In??s.
Subjects/Keywords: LVPR; IDGA; ELISA; Western blot; Semi??rido
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APA (6th Edition):
Sena, G. S. R. d. (2013). Ocorr??ncia da infec????o por lentivirus de pequenos ruminantes em ovinos e caprinos do semi??rido baiano e caracter??sticas deste sistema produtivo na regi??o. (Masters Thesis). Brazil. Retrieved from http://www.repositorio.ufba.br/ri/handle/ri/12190
Chicago Manual of Style (16th Edition):
Sena, Glauber Santos Ribeiro de. “Ocorr??ncia da infec????o por lentivirus de pequenos ruminantes em ovinos e caprinos do semi??rido baiano e caracter??sticas deste sistema produtivo na regi??o.” 2013. Masters Thesis, Brazil. Accessed April 13, 2021.
http://www.repositorio.ufba.br/ri/handle/ri/12190.
MLA Handbook (7th Edition):
Sena, Glauber Santos Ribeiro de. “Ocorr??ncia da infec????o por lentivirus de pequenos ruminantes em ovinos e caprinos do semi??rido baiano e caracter??sticas deste sistema produtivo na regi??o.” 2013. Web. 13 Apr 2021.
Vancouver:
Sena GSRd. Ocorr??ncia da infec????o por lentivirus de pequenos ruminantes em ovinos e caprinos do semi??rido baiano e caracter??sticas deste sistema produtivo na regi??o. [Internet] [Masters thesis]. Brazil; 2013. [cited 2021 Apr 13].
Available from: http://www.repositorio.ufba.br/ri/handle/ri/12190.
Council of Science Editors:
Sena GSRd. Ocorr??ncia da infec????o por lentivirus de pequenos ruminantes em ovinos e caprinos do semi??rido baiano e caracter??sticas deste sistema produtivo na regi??o. [Masters Thesis]. Brazil; 2013. Available from: http://www.repositorio.ufba.br/ri/handle/ri/12190
10.
Gabriele Bin Alves Pereira.
Imunodetecção de antígenos de Escherichia coli em infecção experimental em aves.
Degree: 2012, University of São Paulo
URL: http://www.teses.usp.br/teses/disponiveis/10/10133/tde-05062013-160623/
► A colibacilose é uma enfermidade importante na avicultura por causar prejuízos econômicos e danos à carcaça. Escherichia coli é um micro-organismo oportunista e a complexidade…
(more)
▼ A colibacilose é uma enfermidade importante na avicultura por causar prejuízos econômicos e danos à carcaça. Escherichia coli é um micro-organismo oportunista e a complexidade da infecção se deve a diversidade de sorotipos, variados fatores de virulência e baixa capacidade de estimular a imunidade cruzada. Assim, a compreensão dos mecanismos de virulência que ocorrem no hospedeiro, pode resultar no desenvolvimento de métodos diagnósticos e profiláticos. Neste estudo, 85 pintinhos foram divididos em quatro grupos, sendo três infectados com diferentes sorogrupos de Escherichia coli (O2, O78, O119) e um vacinado com Poulvac® E.coli (Pfizer®). As lesões sugestivas de colibacilose foram avaliadas e qualificadas aos 35 dias de idade, e em seguida realizada a colheita de sangue para a obtenção do soro. Foram obtidas proteínas in vitro dos sorogrupos de E. coli utilizados na infecção experimental e da vacina (mutante de Escherichia coli
O78). Estas foram separadas por eletroforese unidimensional em gel de acrilamida utilizando dodecil sulfato de sódio (SDS) e foram submetidas a reação de western blot com a mistura de soros de cada grupo estudado e também com o pool de soros de aves livres de patógenos específicos (SPF), usadas como controle negativo. O perfil eletroforético revelou a presença de várias proteínas dos diferentes sorogrupos de E. coli quando cultivadas no meio casaminoácido e levedura. Houve diferenças entre os sorotipos, mas também proteínas comuns. Foi possível estabelecer uma relação entre virulência, quantidades de proteínas específicas e taxa de reisolamento.
The colibacillosis is a serious disease in poultry industry to cause economic losses and damage to carcass. Escherichia coli is an opportunistic microorganism and the complexity of infection is due to the diversity of serotypes, several virulence factors and low ability to stimulate cross-immunity. Thus understanding of virulence
mechanisms that occur in the host, may result in development of diagnostic and prophylactic methods. In this study, 85 chicks were divided into four groups being infected with three different serogroups of E. coli (O2, O78, O119) and vaccinated with Poulvac E. coli ® (Pfizer ®). The lesions suggestive of colibacillosis were evaluated at 35 days of age, and then the blood sample are collected to obtain the serum. Proteins were obtained in vitro from serogroups of E. coli used in experimental infection and vaccine (mutant of Escherichia coli O78). These were separated by one-dimensional electrophoresis on acrylamide gels using sodium dodecyl sulfate (SDS) and then reacted by western blot with the pool of sera from each group and also with the pool of sera from specific pathogen free poultry used as negative control. The electrophoretic pattern showed the presence of many proteins of different serogroups of E. coli when grown in the broth casaminoacid and yeast. There
were differences among serotypes, but also common proteins. It was possible to establish a relationship between virulence and…
Advisors/Committee Members: Antônio José Piantino Ferreira, Terezinha Knöbl, Elena Mettifogo.
Subjects/Keywords: Escherichia coli; Western blot; Eletroforese unidimensional; Proteínas; Escherichia coli; Western blot; Proteins; Unidimensional electrophoresis
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APA (6th Edition):
Pereira, G. B. A. (2012). Imunodetecção de antígenos de Escherichia coli em infecção experimental em aves. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/10/10133/tde-05062013-160623/
Chicago Manual of Style (16th Edition):
Pereira, Gabriele Bin Alves. “Imunodetecção de antígenos de Escherichia coli em infecção experimental em aves.” 2012. Masters Thesis, University of São Paulo. Accessed April 13, 2021.
http://www.teses.usp.br/teses/disponiveis/10/10133/tde-05062013-160623/.
MLA Handbook (7th Edition):
Pereira, Gabriele Bin Alves. “Imunodetecção de antígenos de Escherichia coli em infecção experimental em aves.” 2012. Web. 13 Apr 2021.
Vancouver:
Pereira GBA. Imunodetecção de antígenos de Escherichia coli em infecção experimental em aves. [Internet] [Masters thesis]. University of São Paulo; 2012. [cited 2021 Apr 13].
Available from: http://www.teses.usp.br/teses/disponiveis/10/10133/tde-05062013-160623/.
Council of Science Editors:
Pereira GBA. Imunodetecção de antígenos de Escherichia coli em infecção experimental em aves. [Masters Thesis]. University of São Paulo; 2012. Available from: http://www.teses.usp.br/teses/disponiveis/10/10133/tde-05062013-160623/
11.
Douglas Segalla Caragelasco.
Efeito da terapia com células tronco mesenquimais na proteinúria de cães com doença renal crônica.
Degree: 2017, University of São Paulo
URL: http://www.teses.usp.br/teses/disponiveis/10/10136/tde-11042018-104404/
► A proteinúria de origem renal é um indicador de lesão e atua na progressão da doença renal crônica em cães. Na rotina clínica, várias são…
(more)
▼ A proteinúria de origem renal é um indicador de lesão e atua na progressão da doença renal crônica em cães. Na rotina clínica, várias são as recomendações em relação à terapia de manutenção, que visam minimizar ou retardar a progressão da doença. Com o recente progresso na pesquisa sobre a terapia com as células tronco mesenquimais (CTM), tem-se discutido sobre a possibilidade de minimização dos mecanismos inflamatórios e imunológicos de autoperpetuação da lesão renal com a sua utilização, assim a análise sequencial das proteínas urinárias por métodos qualitativos, tais como a eletroforese em gel de poliacrilamida (SDS-PAGE) e a imunodetecção das proteínas por Western Blot pode trazer subsídios para esta avaliação. Portanto, como hipótese, suscita-se que a administração de CTM em cães com DRC possa trazer benefícios com o intuito de minimizar o desenvolvimento da proteinúria, contribuindo para o retardo na velocidade de progressão da doença renal.
Trata-se de um estudo prospectivo, longitudinal e duplo-cego em que foram avaliados 22 cães com DRC, tratados com solução fisiológica (SF) ou CTM e avaliados a cada 30 a 45 dias em 12 momentos, divididos em grupos de acordo com o estágio da doença, grupo A (estágio 2, SF:n=6, CTM:n=3) e grupo B (estágio 3, SF:n=6, CTM:n=7). Observou-se que os cães do Grupo B apresentaram proteinúria mais intensa, maior porcentagem de proteínas de alto peso molecular, maior imunodetecção de albumina, proteínas ligadas ao retinol e a vitamina D e menor imunodetecção de proteína de Tamm-Horsfall ao longo do acompanhamento, quando comparado ao Grupo A. Os resultados obtidos nesse estudo não permitiram definir conclusões contundentes de que a terapia com a CTM tenha trazido alterações importantes que indicassem o seu benefício. Os dados sugerem que os cães nos estágios iniciais da DRC (estágio 2) poderiam ser os mais favorecidos com a referida terapia, entretanto mais estudos contemplando um número maior
de animais, como o maior tempo de acompanhamento devem ser conduzidos para tal investigação.
Protein of renal origin is an indicator of injury and acts on the progression of chronic kidney disease in dogs. In the clinical routine, several recommendations regarding maintenance therapy are aimed at minimizing or delaying the progression of the disease. With the recent progress in the research on mesenchymal stem cell therapy (CTM), it has been discussed the possibility of minimizing the inflammatory and immunological mechanisms of self-perpetuation of the renal lesion with its use, thus the sequential analysis of the urinary proteins by qualitative methods such as polyacrylamide gel electrophoresis (SDS-PAGE) and the immunodetection of proteins by Western blot can prove the efficacy of the therapy. Therefore, as hypothesis, it is suggested that the administration of CTM in dogs with CKD can fetch benefits in order to minimize the development of proteinuria, contributing to the
delay in the rate of progression of renal disease. This is a prospective, longitudinal, double-blind study in…
Advisors/Committee Members: Marcia Mery Kogika, Lucia da Conceição Andrade, Samirah Abreu Gomes, Camila Eleuterio Rodrigues, Talita Rojas Cunha Sanches.
Subjects/Keywords: Western Blot; Canino; Eletroforese; Nefropatia; Proteína Urinária; Canine; Electrophoresis; Nephropathy; Proteinuria; Western Blot
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APA (6th Edition):
Caragelasco, D. S. (2017). Efeito da terapia com células tronco mesenquimais na proteinúria de cães com doença renal crônica. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/10/10136/tde-11042018-104404/
Chicago Manual of Style (16th Edition):
Caragelasco, Douglas Segalla. “Efeito da terapia com células tronco mesenquimais na proteinúria de cães com doença renal crônica.” 2017. Doctoral Dissertation, University of São Paulo. Accessed April 13, 2021.
http://www.teses.usp.br/teses/disponiveis/10/10136/tde-11042018-104404/.
MLA Handbook (7th Edition):
Caragelasco, Douglas Segalla. “Efeito da terapia com células tronco mesenquimais na proteinúria de cães com doença renal crônica.” 2017. Web. 13 Apr 2021.
Vancouver:
Caragelasco DS. Efeito da terapia com células tronco mesenquimais na proteinúria de cães com doença renal crônica. [Internet] [Doctoral dissertation]. University of São Paulo; 2017. [cited 2021 Apr 13].
Available from: http://www.teses.usp.br/teses/disponiveis/10/10136/tde-11042018-104404/.
Council of Science Editors:
Caragelasco DS. Efeito da terapia com células tronco mesenquimais na proteinúria de cães com doença renal crônica. [Doctoral Dissertation]. University of São Paulo; 2017. Available from: http://www.teses.usp.br/teses/disponiveis/10/10136/tde-11042018-104404/
Universidade Federal da Bahia
12.
Virgínia Maria Góes da Silva.
Characterization of immunomodulating factor produced by Leishmania amazonensis and adjuvant derived from babassu (Orbignya phalerata, Mart.), candidates for leishmaniasis vaccine.
Degree: 2009, Universidade Federal da Bahia
URL: http://www.bibliotecadigital.ufba.br/tde_busca/arquivo.php?codArquivo=3522
;
http://www.bibliotecadigital.ufba.br/tde_busca/arquivo.php?codArquivo=3523
► A leishmaniose permanece um importante problema de saúde pública, apesar do intenso esforço visando o desenvolvimento de uma vacina eficaz. No presente estudo foi utilizado…
(more)
▼ A leishmaniose permanece um importante problema de saúde pública, apesar do intenso esforço visando o desenvolvimento de uma vacina eficaz. No presente estudo foi utilizado um modelo murino experimental de leishmaniose. A linhagem de camundongos BALB/c é altamente suscetível à infecção por L. amazonensis e relativamente resistente a L. braziliensis. Portanto, esse modelo poderia contribuir para o estudo de fatores relacionados ao parasito que determinem uma ótima resposta imune do hospedeiro ou pelo contrário, inibir ou desviar a resposta imune, induzindo suscetibilidade. No presente estudo nós demonstramos que a injeção intravenosa do extrato de amastigota de L. amazonensis (extrato La), e não do extrato de amastigota L. braziliensis (extrato Lb) em camundongos BALB/c, pode intensificar a infecção por L. braziliensis. A atividade biológica do extrato La não foi mediada por moléculas anfifílicas e foi inibida pelo pré-tratamento do extrato com inibidores irreversíveis de serino-protease. Esse efeito foi associado com o aumento da produção da IL-4 e a diminuição de IFN-γ por células do linfonodo drenante estimuladas com anti-CD3. Ademais, esse efeito foi ausente em camundongos nocaute para IL-4. Por outro lado, atividade de anticorpo IgG1 anti-Leishmania, detectado por ELISA, foi significantemente superior no grupo de camundongos tratados com o extrato La do que no controle, grupo tratado com salina. A diferença quantitativa na produção de anticorpo IgG1 anti-Leishmania induzido pela administração intravenosa do La extrato levaram a estudos qualitativos, permitindo a detecção de antígenos do parasito relacionados com a evolução da doença (suscetibilidade) ou, em última analise, a um antígeno candidato para o desenvolvimento de uma vacina. A neutralização pelo sistema imune de um fator que induz uma resposta imune inadequada do hospedeiro poderia, pelo menos teoricamente, favorecer uma resposta ótima ou adequada. Esses estudos qualitativos revelaram que camundongos BALB/c que se tornaram suscetíveis a infecção por L. braziliensis através de injeções do extrato La produziram anticorpos IgG1 reconhecendo especificamente um antígeno com peso molecular de 116 kDa. Essa reatividade foi ausente no soro dos camundongos infectados, previamente tratados com o extrato La suplementado com inibidor de protease ou salina. Finalmente, esses estudos demonstraram que o extrato aquoso obtido da farinha do mesocarpo do babaçu (BAE) poderia agir como um adjuvante imunológico para 12 imunização com antígeno de Leishmania através da promoção de uma resposta imune protetora tipo Th1. Esses achados indicam uma nova abordagem que possa resultar no desenvolvimento de uma vacina, associando uma molécula capaz de potencializar a infecção por Leishmania com a atividade de um adjuvante recém descoberto a partir de um produto natural, presente na dieta humana com registro de boa segurança.
The leishmaniasis remain a major public health problem, despite an intensive effort aiming the development of an effective vaccine. In the present study…
Advisors/Committee Members: Amélia Maria Ribeiro de Jesus, Sergio Coutinho Furtado de Mendonca, Lain Carlos Pontes de Carvalho, Neci Matos Soares, Gabriel Grimaldi Filho, José Orivaldo Mengele Júnior.
Subjects/Keywords: leishmaniose; leishmania; imunomodulação; antígeno; babaçu e Western blot; leishmaniasis; leishmania; immunomodulation; antigen; babassu e western blot; IMUNOLOGIA
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APA (6th Edition):
Silva, V. M. G. d. (2009). Characterization of immunomodulating factor produced by Leishmania amazonensis and adjuvant derived from babassu (Orbignya phalerata, Mart.), candidates for leishmaniasis vaccine. (Thesis). Universidade Federal da Bahia. Retrieved from http://www.bibliotecadigital.ufba.br/tde_busca/arquivo.php?codArquivo=3522 ; http://www.bibliotecadigital.ufba.br/tde_busca/arquivo.php?codArquivo=3523
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Silva, Virgínia Maria Góes da. “Characterization of immunomodulating factor produced by Leishmania amazonensis and adjuvant derived from babassu (Orbignya phalerata, Mart.), candidates for leishmaniasis vaccine.” 2009. Thesis, Universidade Federal da Bahia. Accessed April 13, 2021.
http://www.bibliotecadigital.ufba.br/tde_busca/arquivo.php?codArquivo=3522 ; http://www.bibliotecadigital.ufba.br/tde_busca/arquivo.php?codArquivo=3523.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Silva, Virgínia Maria Góes da. “Characterization of immunomodulating factor produced by Leishmania amazonensis and adjuvant derived from babassu (Orbignya phalerata, Mart.), candidates for leishmaniasis vaccine.” 2009. Web. 13 Apr 2021.
Vancouver:
Silva VMGd. Characterization of immunomodulating factor produced by Leishmania amazonensis and adjuvant derived from babassu (Orbignya phalerata, Mart.), candidates for leishmaniasis vaccine. [Internet] [Thesis]. Universidade Federal da Bahia; 2009. [cited 2021 Apr 13].
Available from: http://www.bibliotecadigital.ufba.br/tde_busca/arquivo.php?codArquivo=3522 ; http://www.bibliotecadigital.ufba.br/tde_busca/arquivo.php?codArquivo=3523.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Silva VMGd. Characterization of immunomodulating factor produced by Leishmania amazonensis and adjuvant derived from babassu (Orbignya phalerata, Mart.), candidates for leishmaniasis vaccine. [Thesis]. Universidade Federal da Bahia; 2009. Available from: http://www.bibliotecadigital.ufba.br/tde_busca/arquivo.php?codArquivo=3522 ; http://www.bibliotecadigital.ufba.br/tde_busca/arquivo.php?codArquivo=3523
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Vienna
13.
Schuster, Roswitha.
Anionen-transportierende Polypeptide (OATPs) in Lebertumoren.
Degree: 2010, University of Vienna
URL: http://othes.univie.ac.at/11785/
► Leberkrebs ist eine der häufigsten Tumorerkrankungen weltweit, Männer sind doppelt so oft betroffen als Frauen. Der Grund für die hohe Sterblichkeitsrate liegt darin, dass Leberkrebs…
(more)
▼ Leberkrebs ist eine der häufigsten Tumorerkrankungen weltweit, Männer sind doppelt so oft betroffen als Frauen. Der Grund für die hohe Sterblichkeitsrate liegt darin, dass Leberkrebs erst in einem sehr späten und fortgeschrittenen Stadium erkannt wird. Zudem ist er meist resistent gegen Chemotherapie. Die Effizienz zytostatischer Substanzen beruht weitgehend auf deren Konzentration in den Zellen, die wiederrum unter anderem von der Aufnahme und dem Abtransport dieser Substanzen durch spezifische Wirkstofftransporter abhängt. Obwohl die Überexpression des ABC-Efflux Transporters zu erhöhten Transportraten aus der Zelle hinaus führt, könnte die intrazelluläre Wirkstoffakkumulation durch eine effektive Aufnahme mittels charakteristischer Aufnahmetransporter kompensiert werden. In dieser Hinsicht stellen die Transporter der Organic Anion Transporting Polypeptides (OATP) eine interessante Gruppe dar, da sie in der Lage sind ein breites Spektrum an vorwiegend anionischen, endogenen und exogenen Substanzen, die für eine Chemotherapie im klinischem Gebrauch sind, in verschiedene Zellen des menschlichen Körpers zu transportieren. In der Leber spielen sie eine wichtige Rolle in der Aufnahme, Verteilung, Metabolisierung und Ausscheidung von Arzneistoffen. Bis heute konnten 11 humane OATPs ermittelt werden. Das Ziel meiner Arbeit war, die Expression der OATP1B1 und OATP2A1, in gesunden und kranken Lebergewebe mittels Western Blot Analyse und anschließender Immundetektion zu ermitteln und die Daten mit vorhandenen Real Time PCR Ergebnissen zu vergleichen. Es konnte gezeigt werden, dass in der Membranfraktion dieser Tumore immunoreaktive Banden von OATP1B1 bei einem Molekulargewicht von 70 kDa aufscheinen. Die OATP1B1 Level waren in allen Patientenproben in normalem Gewebe höher als in malignem Gewebe. Immunoreaktive Banden des OATP2A1 konnten in der Membranfraktion bei einem Molekulargewicht von 50 kDa detektiert werden, während im Gesamtextrakt die immunoreaktive Fraktion bei einem Molekulargewicht von 50 kDa als auch bei 70 kDa sichtbar gemacht werden konnte. Zudem zeigten sich höhere Expressionslevel des 50 kDa OATP2A1 in malignem Gewebe während die Expressionslevel des 70 kDa Proteins in Normalgewebe höher erschienen. Bei beiden Transportern korrelierte die mRNA Expression mit den Ergebnissen der Proteinexpression. Zusammenfassend zeigt diese Arbeit individuelle Expressionsmuster von OATP1B1 und OATP2A1 in malignem und nicht-malignem Lebergewebe.
Hepatocellular carcinomas are the sixth most common cause of cancer worldwide and these tumors are two-times more common in men than in women. One reason for the high mortality rate is that in most cases, hepatocellular carcinomas are recognized at advanced stage. Additionally, these tumors are highly resistant to standard chemotherapy regimens. The efficiency of any cytostatic drug therapy critically depends on the concentration of anticancer drugs in the cells which results from the uptake and efflux via specific drug transporters and the degree of biotransformation.…
Subjects/Keywords: 44.40 Pharmazie, Pharmazeutika; Anionen-Transportierende Polypeptide / OATP / Lebertumor / Western Blot / Chemotherapie; Anion-transporting polypeptides / OATP / liver cancer / western blot / chemotherapy
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APA (6th Edition):
Schuster, R. (2010). Anionen-transportierende Polypeptide (OATPs) in Lebertumoren. (Thesis). University of Vienna. Retrieved from http://othes.univie.ac.at/11785/
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Schuster, Roswitha. “Anionen-transportierende Polypeptide (OATPs) in Lebertumoren.” 2010. Thesis, University of Vienna. Accessed April 13, 2021.
http://othes.univie.ac.at/11785/.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Schuster, Roswitha. “Anionen-transportierende Polypeptide (OATPs) in Lebertumoren.” 2010. Web. 13 Apr 2021.
Vancouver:
Schuster R. Anionen-transportierende Polypeptide (OATPs) in Lebertumoren. [Internet] [Thesis]. University of Vienna; 2010. [cited 2021 Apr 13].
Available from: http://othes.univie.ac.at/11785/.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Schuster R. Anionen-transportierende Polypeptide (OATPs) in Lebertumoren. [Thesis]. University of Vienna; 2010. Available from: http://othes.univie.ac.at/11785/
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Brno University of Technology
14.
Šupák, Marek.
Příprava myších monoklonálních protilátek proti cyklin-dependentní kináze 13: Preparation of mouse monoclonal antobodies against cyclin-dependent kinase.
Degree: 2019, Brno University of Technology
URL: http://hdl.handle.net/11012/177430
► The aim of this master‘s thesis is to prepare a monoclonal antibody against cyclin-dependent kinase 13 (CDK13). The theoretical part focuses on antigen-antibody binding, which…
(more)
▼ The aim of this master‘s thesis is to prepare a monoclonal antibody against cyclin-dependent kinase 13 (CDK13). The theoretical part focuses on antigen-antibody binding, which is essential for the use of monoclonal antibodies in the determination of CDK13 as well as the transcription that this kinase affects. This section is also devoted to
Western blot and ELISA methods for detection of newly generated antibodies. Furthermore, the antibodies and the antigen definition are stated, which are later on discussed. The practical part is devoted to the preparation of antigen - its isolation and purification on a peristaltic pump. It also addresses immunization, its course, and the amount of antigen used to immunize mice. After immunization, the work focuses on fusion of sp-2 cells and splenocytes, which were first removed from the immunized mouse and purified. After the fusion alone, selection of hybridomas on HAT selection medium is mentioned, followed by detection first by ELISA and later by
Western blotting. The resulting hybridomas with positive ELISA response are frozen for further testing at the Veterinary Research Institute in Brno, where the entire practical part of this thesis was carried out. These frozen hybridomas are further tested by immunoprecipitation to conclude this thesis.
Advisors/Committee Members: Kohoutek,, Jiří (advisor), Ševčíková,, Sabina (referee).
Subjects/Keywords: CDK13; hybridomová technológia; primárne protilátky; Western blot; ELISA; CDK13; hybridoma technology; primary antibody; western blot; ELISA
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APA ·
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APA (6th Edition):
Šupák, M. (2019). Příprava myších monoklonálních protilátek proti cyklin-dependentní kináze 13: Preparation of mouse monoclonal antobodies against cyclin-dependent kinase. (Thesis). Brno University of Technology. Retrieved from http://hdl.handle.net/11012/177430
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Šupák, Marek. “Příprava myších monoklonálních protilátek proti cyklin-dependentní kináze 13: Preparation of mouse monoclonal antobodies against cyclin-dependent kinase.” 2019. Thesis, Brno University of Technology. Accessed April 13, 2021.
http://hdl.handle.net/11012/177430.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Šupák, Marek. “Příprava myších monoklonálních protilátek proti cyklin-dependentní kináze 13: Preparation of mouse monoclonal antobodies against cyclin-dependent kinase.” 2019. Web. 13 Apr 2021.
Vancouver:
Šupák M. Příprava myších monoklonálních protilátek proti cyklin-dependentní kináze 13: Preparation of mouse monoclonal antobodies against cyclin-dependent kinase. [Internet] [Thesis]. Brno University of Technology; 2019. [cited 2021 Apr 13].
Available from: http://hdl.handle.net/11012/177430.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Šupák M. Příprava myších monoklonálních protilátek proti cyklin-dependentní kináze 13: Preparation of mouse monoclonal antobodies against cyclin-dependent kinase. [Thesis]. Brno University of Technology; 2019. Available from: http://hdl.handle.net/11012/177430
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
15.
Pereira, Heloisa Aparecida Barbosa da Silva.
Efeito do tempo de tratamento e da dose de fluoreto administrada cronicamente na expressão proteica em fígado de ratos.
Degree: 2015, Universidade Federal de São Carlos; Câmpus São Carlos; Programa de Pós-Graduação em Genética Evolutiva e Biologia Molecular – PPGGEv; UFSCar
URL: https://repositorio.ufscar.br/handle/ufscar/7290
► Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
In a previous study conducted by our group, it was noticed that fluoride (F)…
(more)
▼ Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
In a previous study conducted by our group, it was noticed that fluoride (F) can induce changes in the expression of several liver proteins. Reports in the literature suggest that the changes caused by F in the body are dose- and time-dependent. The objective of this study was to analyze the effect of different F concentrations, exposure time to this ion and concomitant exposure to a high calorie diet in the metabolism of lipids and protein expression in the liver of rats. The study was divided into 2 steps. The first step included 72 21-day-old male Wistar rats that were divided into 2 groups (n=36) according to the diet administered (AIN-93M and Presence). Each group was further divided according to the duration of the treatment (20 or 60 days). In addition, each these was divided into 3 subgroups (n=6), according to the concentration of F administrated in the drinking water, as
follows: 0 mg/L (control), 15 mg/L or 50 mg/L. After the experimental period, the animals were anesthetized and the liver and blood were collected. F analysis in plasma and liver tissue was done. Part of the liver was fixed for histological analysis. Lipids were analyzed in plasma and triglycerides were analyzed in the liver. Expressions of proteins were evaluated in the liver by Western blotting. In the second step the only Presence diet were use and the groups experimental the same as previously described. At the end of experimental period, liver and plasma were collected. F concentration in plasma and liver were analyzed. Liver proteins were extracted and prepared for mass spectrometry analysis. Proteins were sequenced and identified. The analysis of F concentrations indicated a doseresponse increase in plasma, regardless the time of exposure to F and type of diet. F concentrations in the liver were higher in the groups receiving 50 ppm F in respect to control. Administration of F
altered the lipid profile, with a reduction in TGA in plasma and increase in HDL when the hypercaloric diet was used. The expression of GRP78, ERP29 and SOD2 and Apo-E was altered by F, under the influence of time and type of diet administered. For the groups receiving 50 mgF/L, it was observed an increase in the concentration of proteins related to the defense against oxidative stress and ER stress. For the group that received the concentration of 15 mgF/L, changes in structural proteins, mitochondrial proteins and proteins related to cell proliferation were observed, depending on the time of administration. The results suggest an adaptive mechanism of liver upon exposure to F, which seems to be related to the activation of proteins related to maintenance of homeostasis and that fight against oxidative stress and ER stress caused by this ion.
Em trabalho prévio realizado pelo nosso grupo foi observado que o fluoreto (F) pode provocar alterações na expressão de várias proteínas
hepáticas. Relatos na literatura sugerem que as alterações causadas pelo F no organismo são dose e …
Advisors/Committee Members: Buzalaf, Marília Afonso Rabelo.
Subjects/Keywords: Fluoreto; Perfil lipídico; Análise proteômica; Fígado; Western blot; Western blot; Fluoride; Lipid profile; Proteomic analysis; Liver; CIENCIAS BIOLOGICAS
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APA (6th Edition):
Pereira, H. A. B. d. S. (2015). Efeito do tempo de tratamento e da dose de fluoreto administrada cronicamente na expressão proteica em fígado de ratos. (Doctoral Dissertation). Universidade Federal de São Carlos; Câmpus São Carlos; Programa de Pós-Graduação em Genética Evolutiva e Biologia Molecular – PPGGEv; UFSCar. Retrieved from https://repositorio.ufscar.br/handle/ufscar/7290
Chicago Manual of Style (16th Edition):
Pereira, Heloisa Aparecida Barbosa da Silva. “Efeito do tempo de tratamento e da dose de fluoreto administrada cronicamente na expressão proteica em fígado de ratos.” 2015. Doctoral Dissertation, Universidade Federal de São Carlos; Câmpus São Carlos; Programa de Pós-Graduação em Genética Evolutiva e Biologia Molecular – PPGGEv; UFSCar. Accessed April 13, 2021.
https://repositorio.ufscar.br/handle/ufscar/7290.
MLA Handbook (7th Edition):
Pereira, Heloisa Aparecida Barbosa da Silva. “Efeito do tempo de tratamento e da dose de fluoreto administrada cronicamente na expressão proteica em fígado de ratos.” 2015. Web. 13 Apr 2021.
Vancouver:
Pereira HABdS. Efeito do tempo de tratamento e da dose de fluoreto administrada cronicamente na expressão proteica em fígado de ratos. [Internet] [Doctoral dissertation]. Universidade Federal de São Carlos; Câmpus São Carlos; Programa de Pós-Graduação em Genética Evolutiva e Biologia Molecular – PPGGEv; UFSCar; 2015. [cited 2021 Apr 13].
Available from: https://repositorio.ufscar.br/handle/ufscar/7290.
Council of Science Editors:
Pereira HABdS. Efeito do tempo de tratamento e da dose de fluoreto administrada cronicamente na expressão proteica em fígado de ratos. [Doctoral Dissertation]. Universidade Federal de São Carlos; Câmpus São Carlos; Programa de Pós-Graduação em Genética Evolutiva e Biologia Molecular – PPGGEv; UFSCar; 2015. Available from: https://repositorio.ufscar.br/handle/ufscar/7290
Universidade Federal da Bahia
16.
Lia Muniz Barretto Fernandes.
Doenças de Newcastle: padronização de testes sorológicos para o diagnostico em avestruzes (Struthio Camelus) e avaliação soroepidemiológica nos Estados da Bahia e de São Paulo.
Degree: 2006, Universidade Federal da Bahia
URL: http://www.bibliotecadigital.ufba.br/tde_busca/arquivo.php?codArquivo=548
► Newcastle Disease is a highly contagious viral infection, which attacks several avian species, and represents one of the most important diseases for the modern poultry…
(more)
▼ Newcastle Disease is a highly contagious viral infection, which attacks several avian species, and represents one of the most important diseases for the modern poultry industry. Because of the relevance of the poultry industry in Brazil and the boom in ostrich rearing, it?s necessary the development of control strategies and it depends on the development of practical and reliable tools for diagnosis. Such tools are available for chickens; however specific tests were not developed for ostriches yet. The aim of the present work is to standardize serological tests for the detection of antibodies against the Newcastle disease in ostriches and assess the seroepidemiological situation of flocks located in Bahia and São Paulo. The standardization of haemagglutination inhibition (HI) revealed interference of the type of used red blood cells and demonstrated the need of the use of erythrocytes of the same species or, alternatively, of turkeys. Indirect ELISA were developed or modified for this species and, in spite of they present high correlation amongst themselves, they demonstrated low correlation with HI. "Western blot" and "dotblot" were developed, and that can be useful in evaluation of the immune response and to make possible the implantation of control programs. The samples analyzed revealed the presence of antibodies against NDV and, as these animals were not vaccinated, the hypothesis that the ostriches are in contact with the vaccine virus or wild virus is reinforced.
Doença de Newcastle é uma enfermidade viral aguda, altamente contagiosa, que acomete aves de várias espécies, considerada como uma das doenças mais importantes para a indústria avícola moderna. Ferramentas para diagnóstico e controle estão disponíveis para galinhas, porém ainda não foram desenvolvidos testes específicos para avestruzes. O presente trabalho visou padronizar testes sorológicos para a detecção de anticorpos contra a Doença de Newcastle em avestruzes e avaliar a situação soroepidemiológica de plantéis do estado da Bahia e de São Paulo. A padronização da técnica da Inibição da Hemaglutinação revelou interferência do tipo de eritrócito utilizado e demonstrou a necessidade do uso de hemácias da mesma espécie ou, alternativamente, de perus. Testes de ELISA indiretos foram desenvolvidos ou modificados para a utilização nesta espécie e, apesar de apresentarem alta correlação entre si, demonstraram baixa correlação com a HI. Foram desenvolvidos ainda os testes ?western blot? e ?dotblot?, que podem auxiliar na avaliação da resposta imune e facilitar a implantação de programas de controle. As amostras séricas analisadas revelaram a presença de anticorpos e, a ausência de vacinação dos animais avaliados, reforça a hipótese de que as avestruzes estão em contato com o vírus vacinal ou vírus de campo.
Advisors/Committee Members: Ruben Pablo Schocken-Iturrino, Elizabeth Santin, Luciano Doretto Júnior, Antonio Carlos Paulillo, Nilce Maria Soares Queiroz Gama, Songeli Menezes Freire.
Subjects/Keywords: Newcastle disease; ostriches; western-blott; dot-blot; inibição da hemaglutinação; ELISA; avestruzes; doença de newcastle; Ciências Biológicas; HI; ELISA; Dot-Blot; Western-blot
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APA (6th Edition):
Fernandes, L. M. B. (2006). Doenças de Newcastle: padronização de testes sorológicos para o diagnostico em avestruzes (Struthio Camelus) e avaliação soroepidemiológica nos Estados da Bahia e de São Paulo. (Thesis). Universidade Federal da Bahia. Retrieved from http://www.bibliotecadigital.ufba.br/tde_busca/arquivo.php?codArquivo=548
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Fernandes, Lia Muniz Barretto. “Doenças de Newcastle: padronização de testes sorológicos para o diagnostico em avestruzes (Struthio Camelus) e avaliação soroepidemiológica nos Estados da Bahia e de São Paulo.” 2006. Thesis, Universidade Federal da Bahia. Accessed April 13, 2021.
http://www.bibliotecadigital.ufba.br/tde_busca/arquivo.php?codArquivo=548.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Fernandes, Lia Muniz Barretto. “Doenças de Newcastle: padronização de testes sorológicos para o diagnostico em avestruzes (Struthio Camelus) e avaliação soroepidemiológica nos Estados da Bahia e de São Paulo.” 2006. Web. 13 Apr 2021.
Vancouver:
Fernandes LMB. Doenças de Newcastle: padronização de testes sorológicos para o diagnostico em avestruzes (Struthio Camelus) e avaliação soroepidemiológica nos Estados da Bahia e de São Paulo. [Internet] [Thesis]. Universidade Federal da Bahia; 2006. [cited 2021 Apr 13].
Available from: http://www.bibliotecadigital.ufba.br/tde_busca/arquivo.php?codArquivo=548.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Fernandes LMB. Doenças de Newcastle: padronização de testes sorológicos para o diagnostico em avestruzes (Struthio Camelus) e avaliação soroepidemiológica nos Estados da Bahia e de São Paulo. [Thesis]. Universidade Federal da Bahia; 2006. Available from: http://www.bibliotecadigital.ufba.br/tde_busca/arquivo.php?codArquivo=548
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Texas A&M University
17.
Zriba, Slim.
Towards the Development of an Improved Vaccine for Swine Against Brucellosis.
Degree: MS, Biomedical Sciences, 2017, Texas A&M University
URL: http://hdl.handle.net/1969.1/161643
► Swine brucellosis is a zoonotic disease with variable incidence rates in domestic and feral populations. It is a serious public health issue due to the…
(more)
▼ Swine brucellosis is a zoonotic disease with variable incidence rates in domestic and feral populations. It is a serious public health issue due to the potential for spillover not only between infected feral and domestic swine but also cattle and humans. Previous studies published by our laboratory has demonstrated the cross-protective immunity elicited by the S19ΔvjbR live attenuated vaccine in mice against challenge with B. abortus, B. melitensis or B. suis, suggesting its potential use as a vaccine against multiple Brucella species. To date there is no effective vaccine used to prevent brucellosis in swine. This prompted us to study the potential use of S19 ΔvjbR as a vaccine candidate in this natural host by evaluating its safety and humoral response in pregnant swine. Fifteen pregnant gilts at fifty days of gestation (midgestation) were divided into four groups and vaccinated subcutaneously with 1X10
10 CFU of either; 1) strain S19; 2) S19ΔvjbR encapsulated in alginate microspheres 3) unencapsulated S19ΔvjbR, or 4) empty capsules as a control. Interestingly, none of the animals vaccinated with either S19 or S19ΔvjbR aborted or demonstrated any adverse side effect associated with vaccination. No bacteria was recovered from any of the tissues examined supporting the lack of histopathological changes in major organs either in piglets or in gilts. Vaginal swab culture from vaccinated animals showed no bacterial growth on Farrell’s agar medium. RBT and IgG iELISA tests were found substantial at 2 week (k= 0.8) and 4 week (k=0.65) post-vaccination suggesting that RBT can be used during this interval as a good tool to confirm the immunization of animals with the different vaccine strains. Different responses of gilts sera against purified vjbR protein at pre-vaccination and two week post-vaccination raised the question of specificity of the vjbR as marker and its inability to validate DIVA capabilities of the ΔvjbR vaccine candidates.
Advisors/Committee Members: Arenas, Angela (advisor), Ficht, Thomas A. (committee member), Welsh, C. Jane (committee member).
Subjects/Keywords: Abortion; brucellosis; vaccination; iELISA; Western blot; Rose Bengal Test; DIVA; ΔvjbR
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APA ·
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MLA ·
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APA (6th Edition):
Zriba, S. (2017). Towards the Development of an Improved Vaccine for Swine Against Brucellosis. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/161643
Chicago Manual of Style (16th Edition):
Zriba, Slim. “Towards the Development of an Improved Vaccine for Swine Against Brucellosis.” 2017. Masters Thesis, Texas A&M University. Accessed April 13, 2021.
http://hdl.handle.net/1969.1/161643.
MLA Handbook (7th Edition):
Zriba, Slim. “Towards the Development of an Improved Vaccine for Swine Against Brucellosis.” 2017. Web. 13 Apr 2021.
Vancouver:
Zriba S. Towards the Development of an Improved Vaccine for Swine Against Brucellosis. [Internet] [Masters thesis]. Texas A&M University; 2017. [cited 2021 Apr 13].
Available from: http://hdl.handle.net/1969.1/161643.
Council of Science Editors:
Zriba S. Towards the Development of an Improved Vaccine for Swine Against Brucellosis. [Masters Thesis]. Texas A&M University; 2017. Available from: http://hdl.handle.net/1969.1/161643
Texas A&M University
18.
Lin, Huaiying 1986-.
Expression and Evaluation of Recombinant Babesia bovis Antigens of Vaccine Potential Against Tick Fever in Cattle.
Degree: MS, Biomedical Sciences, 2013, Texas A&M University
URL: http://hdl.handle.net/1969.1/149237
► Babesia bovis is a causative agent of bovine babesiosis and is transmitted by vector ticks, Rhipicephalus (Boophilus) spp. The disease has a high mortality rate…
(more)
▼ Babesia bovis is a causative agent of bovine babesiosis and is transmitted by vector ticks, Rhipicephalus (Boophilus) spp. The disease has a high mortality rate in susceptible cattle, causing serious economic loss. At present, the only commercial vaccine is culture-based with limited availability. No effective molecular vaccine has been developed to date. Generating a vaccine with specific critical epitopes responsible for protection against B. bovis is critically important. Immunity against B. bovis requires both innate and adaptive responses, with antigen-specific CD4+ T cells essential to the latter through production of IFN-γ. Fourteen B. bovis proteins were selected as putative vaccine candidates and their full-length genes cloned for recombinant protein production intended for evaluating peripheral blood mononuclear cell IFN-γ secretion level from experimentally infected animals in ELISPOT. All proteins expressed in insoluble form (inclusion bodies) and could not be purified. B. bovis genes were then truncated to exclude signal peptide and transmembrane regions, then cloned and expressed using pET101/D-TOPO in Escherichia coli to obtain soluble, useable proteins. Only recombinant B. bovis MSA1, MSA2b and MSA2a1 proteins were successfully expressed in soluble form. These proteins induce invasion-blocking antibodies in immunized cattle, are hypothesized to elicit protection in susceptible animals, but were previously studied by others.
Due to failure to produce new candidates to assay, the animal experiments were not performed. Instead, sera from field-infected cattle were assayed for reactivity against the MSA proteins by indirect immunofluorescent antibody (IFA) and
western blot (WB) analysis. Field sera from South Texas (#41) and the Mexican Yucatan (#6,9 and #11) along with positive and negative controls were tested. In IFA test, cattle #6, #9 and #41 were positive while #11 was negative. In WB, #41 and #6 reacted with the recombinant MSA proteins and with control B. bovis whole parasite lysate. However, both #11 and #9 had no signal in WB, although the latter was positive in IFA. Several theories may explain this phenomenon, such as the different preparation process of the antigen in the two tests, strain differences between sera and test antigens, or the different design and nature of each test.
Advisors/Committee Members: Holman, Patricia J (advisor), Mwangi, Waithaka (committee member), Teel, Pete D (committee member), Freeman, Jeanne (committee member).
Subjects/Keywords: Babesia bovis; MSA; protein expression in E. coli; Western Blot
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APA (6th Edition):
Lin, H. 1. (2013). Expression and Evaluation of Recombinant Babesia bovis Antigens of Vaccine Potential Against Tick Fever in Cattle. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/149237
Chicago Manual of Style (16th Edition):
Lin, Huaiying 1986-. “Expression and Evaluation of Recombinant Babesia bovis Antigens of Vaccine Potential Against Tick Fever in Cattle.” 2013. Masters Thesis, Texas A&M University. Accessed April 13, 2021.
http://hdl.handle.net/1969.1/149237.
MLA Handbook (7th Edition):
Lin, Huaiying 1986-. “Expression and Evaluation of Recombinant Babesia bovis Antigens of Vaccine Potential Against Tick Fever in Cattle.” 2013. Web. 13 Apr 2021.
Vancouver:
Lin H1. Expression and Evaluation of Recombinant Babesia bovis Antigens of Vaccine Potential Against Tick Fever in Cattle. [Internet] [Masters thesis]. Texas A&M University; 2013. [cited 2021 Apr 13].
Available from: http://hdl.handle.net/1969.1/149237.
Council of Science Editors:
Lin H1. Expression and Evaluation of Recombinant Babesia bovis Antigens of Vaccine Potential Against Tick Fever in Cattle. [Masters Thesis]. Texas A&M University; 2013. Available from: http://hdl.handle.net/1969.1/149237
University of Waikato
19.
Hardie, Sarah Elyse.
Expression and Purification of Recombinant VMO1 Protein
.
Degree: 2015, University of Waikato
URL: http://hdl.handle.net/10289/9609
► Hearing loss is a common sensory deficit that affects more than 275 million people worldwide. Identifying and understanding the genetic causes which underlie hearing loss…
(more)
▼ Hearing loss is a common sensory deficit that affects more than 275 million people worldwide. Identifying and understanding the genetic causes which underlie hearing loss can lead to improved diagnostic and treatment strategies. The biological function of Vitelline Membrane Outer Layer 1 homolog (chicken) (VMO1) is of particular interest as the mRNA transcript was previously found to be uniquely expressed and highly abundant in the mouse inner ear.
The aim of this research was to design, clone, express and purify a recombinant VMO1 protein containing a His Tag using the VMO1 gene amplified from human cell lines. The VMO1 gene was cloned into the pET28b(+) expression system and bacterial cells were manipulated to express the recombinant VMO1 protein through IPTG induction. The resulting recombinant protein was then purified using a nickel affinity gel. Finally, a commercial VMO1 antibody was tested and validated using the purified recombinant VMO1 protein using
western blot analysis.
Bioinformatics analysis was undertaken to develop primers to amplify cDNA of two isoforms of the VMO1 gene in human cell lines, isoform 1 and isoform 3. Both isoforms of the VMO1 gene were found to be expressed in two commercial in vitro human cancer cell lines; monocytic leukaemia and breast cancer. Following amplification, the isoforms were sub cloned into T-tailed vectors, with isoform 1 being successfully ligated into the pET28b(+) expression vector. Sequencing data confirmed the amplified cloned PCR inserts were VMO1 transcripts, and the predicted translated sequence was in-frame with the His Tag, resulting in a recombinant protein that was 20 kDa in size.
Expression of the recombinant VMO1 protein was attempted using three bacterial expression strains with successful expression of a 20 kDa protein occurring in the Rosetta™ (DE3) pLysS strain. The recombinant VMO1 protein formed inclusion bodies within bacterial cells following over expression.
Western blot analysis of non purified protein samples showed that the 6X His antibody recognised a 20 kDa recombinant protein as expected. However, the commercially available VMO1 antibody detected two bands approximately 20 and 35 kDa in size.
In the future, it is recommended that optimisation of recombinant protein folding methodology, large scale protein induction and purification of the VMO1 protein be completed. In addition, it is recommended to validate the VMO1 antibody by testing in vitro human cancer cell lines for the expression of VMO1 protein. Once the specificity of the VMO1 antibody has been demonstrated by
western blot analysis, functional assays could be performed to determine the biological function of VMO1. This may provide further insight into the function of VMO1 in the mammalian inner ear and the role it may play in hearing.
Advisors/Committee Members: Peters, Linda (advisor).
Subjects/Keywords: VMO1;
Western blot;
Antibodies;
Recombinant protein;
Hearing loss;
pET system
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APA ·
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MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Hardie, S. E. (2015). Expression and Purification of Recombinant VMO1 Protein
. (Masters Thesis). University of Waikato. Retrieved from http://hdl.handle.net/10289/9609
Chicago Manual of Style (16th Edition):
Hardie, Sarah Elyse. “Expression and Purification of Recombinant VMO1 Protein
.” 2015. Masters Thesis, University of Waikato. Accessed April 13, 2021.
http://hdl.handle.net/10289/9609.
MLA Handbook (7th Edition):
Hardie, Sarah Elyse. “Expression and Purification of Recombinant VMO1 Protein
.” 2015. Web. 13 Apr 2021.
Vancouver:
Hardie SE. Expression and Purification of Recombinant VMO1 Protein
. [Internet] [Masters thesis]. University of Waikato; 2015. [cited 2021 Apr 13].
Available from: http://hdl.handle.net/10289/9609.
Council of Science Editors:
Hardie SE. Expression and Purification of Recombinant VMO1 Protein
. [Masters Thesis]. University of Waikato; 2015. Available from: http://hdl.handle.net/10289/9609
Universidade Nova
20.
CALISTO, Daniela Cristina Portugal.
Imunidade em Malária: contribuição para o estudo de anticorpos em indivíduos com estadia em zona endémica.
Degree: 2015, Universidade Nova
URL: https://www.rcaap.pt/detail.jsp?id=oai:run.unl.pt:10362/19842
► A malária é uma doença infecciosa causada por um protozoário do género Plasmodium. A sua transmissão ao homem é efectuada sobretudo pela picada da fêmea…
(more)
▼ A malária é uma doença infecciosa causada por um protozoário do género Plasmodium. A sua transmissão ao homem é efectuada sobretudo pela picada da fêmea do mosquito Anopheles, podendo ser também transmitida por via transfusional e congénita.
Tendo em conta as zonas endémicas de malária, 3,2 mil milhões de pessoas estão em risco de infecção, sendo a África Subsariana a região mais afectada. No entanto, com o aumento das viagens internacionais, nomeadamente para países tropicais, aumenta o risco de importação de malária para zonas não endémicas, pelo que esta doença deve continuar a ser vigiada cuidadosamente.
O desenvolvimento de imunidade protectora é um processo complexo e moroso, sendo necessárias repetidas exposições ao parasita.Uma vez adquirida, essa imunidade pode ser perdida se o indivíduo permanecer durante algum tempo fora de zona endémica de malária. No entanto, não é claro o período de persistência dos anticorpos no sangue, quando o sistema imune do indivíduo deixa de ser estimulado pelo contacto com o parasita.
Em estudos anteriores, foram verificados anticorpos anti-Plasmodium spp. em indivíduos cujas estadias em zona endémica de malária ocorreram 10 ou mais anos antes da sua participação no estudo.
Assim, o objectivo geral deste trabalho é investigar a natureza biológica das imunoglobulinas (subclasses e tempo de persistência na circulação sanguínea) e identificar as proteínas parasitárias responsáveis pela sua estimulação, em indivíduos com estadia prévia em zona endémica.
De acordo com o resultado serológico por ensaios imunoenzimáticos (ELISA) distinguiram-se duas populações: com (n=76) e sem (n=242) reactividade serológica para Plasmodium spp.
Os resultados serológicos obtidos foram relacionados com as características socio-demográficas, história de malária e número de estadias/viagens a zona endémica, não se tendo encontrado diferenças estatisticamente significativas.
Nos indivíduos com resultado serológico positivo para Plasmodium spp. (n=76) procedeu-se à caracterização das subclasses de imunoglobulinas, IgM (n=11) e IgG (n=51) anti-P. falciparum.
Com base nos resultados de PCR, realizados para identificar infecções em indivíduos com teste serológico positivo para Plasmodium spp. (n=76), dois grupos surgiram: i) grupo com infecção actual (n=8) e ii) grupo com infecção ocorrida no passado (n=68).
As amostras com reactividade serológica para anticorpos anti-P. falciparum foram analisadas por
Western Blot, a fim de se identificarem os antigénios responsáveis pela reactividade serológica dos anticorpos. As proteínas identificadas encontram-se aproximadamente entre o peso molecular de 30 – 60 e 70 – 100 kDa.
A análise dos perfis proteicos demonstrou maior reactividade no grupo de infecção actual do que no grupo de infecção ocorrida no passado. Não obstante, o grupo de participantes com infecção actual apresenta um perfil proteico semelhante entre si,
xii
enquanto que no grupo de infecção ocorrida no passado este perfil é heterogéneo entre os indivíduos.
Este trabalho poderá ser o ponto de partida para…
Advisors/Committee Members: TEODÓSIO, Rosa, SILVA, Marcelo Sousa.
Subjects/Keywords: Malária; Plasmodium falciparum; Anticorpos anti-Plasmodium spp; Viagens; ELISA; Western Blot
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APA ·
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MLA ·
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to Zotero / EndNote / Reference
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APA (6th Edition):
CALISTO, D. C. P. (2015). Imunidade em Malária: contribuição para o estudo de anticorpos em indivíduos com estadia em zona endémica. (Thesis). Universidade Nova. Retrieved from https://www.rcaap.pt/detail.jsp?id=oai:run.unl.pt:10362/19842
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
CALISTO, Daniela Cristina Portugal. “Imunidade em Malária: contribuição para o estudo de anticorpos em indivíduos com estadia em zona endémica.” 2015. Thesis, Universidade Nova. Accessed April 13, 2021.
https://www.rcaap.pt/detail.jsp?id=oai:run.unl.pt:10362/19842.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
CALISTO, Daniela Cristina Portugal. “Imunidade em Malária: contribuição para o estudo de anticorpos em indivíduos com estadia em zona endémica.” 2015. Web. 13 Apr 2021.
Vancouver:
CALISTO DCP. Imunidade em Malária: contribuição para o estudo de anticorpos em indivíduos com estadia em zona endémica. [Internet] [Thesis]. Universidade Nova; 2015. [cited 2021 Apr 13].
Available from: https://www.rcaap.pt/detail.jsp?id=oai:run.unl.pt:10362/19842.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
CALISTO DCP. Imunidade em Malária: contribuição para o estudo de anticorpos em indivíduos com estadia em zona endémica. [Thesis]. Universidade Nova; 2015. Available from: https://www.rcaap.pt/detail.jsp?id=oai:run.unl.pt:10362/19842
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
21.
박, 영준.
Differential expression of beta 2 integrins in Korean Behcet’s disease patients.
Degree: 2014, Ajou University
URL: http://repository.ajou.ac.kr/handle/201003/10921
;
http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000017921
► Background and objectives: Beta 2 integrins (CD11/CD18) are cell adhesion molecules that play a major role in cell migration to inflammatory sites. They also induce…
(more)
▼ Background and objectives: Beta 2 integrins (CD11/CD18) are cell adhesion molecules that play a major role in cell migration to inflammatory sites. They also induce cellular signaling, subsequently leading to cytokine production. On our preliminary strudy, we demonstrated possible alteration in susceptibility to Behcet’s disease (BD) according to single nucleotide polymorphisms of CD11a, CD11c and CD18.Thus, our aim was to investigate the possible relationship between beta 2 integrins expression and BD.
Materials and methods: BD patients (n=51) consisting of 22 active patients and 29 inactive patients, 20 recurrent aphthous ulcer (RAU) patients and 23 healthy controls (HC) were included. The expression of CD11a, CD11b, CD11c, as well as CD4, CD8, CD18 and CD56 in peripheral blood mononuclear cells was assessed by flow cytometry. The transcriptional level expression of CD11a and CD11c was assessed by reverse transcription polymerase charin reaction.Western blot analysis was performed to evaluate CD11a and CD11c protein. Immunohistochemical analysis of CD11a and CD11c was done on erythema nodosum like lesions of 13 BD patients and 14 idiopathic erythema nodosum lesions.
Results: On flow cytometry, the surface expression of CD3 was significantly increased whereas CD4 expression was significantly decreased in BD patients. Expression of CD11a was significanty increased, in contrast to decreased expression of CD11b. RAU patients showed significant decrease in mRNA level of CD11a whereas BD patients did not, compared to the HC. CD11c expression was significantly higher in active BD group compared to the inactive group. CD11c mRNA expression was decreased in BD patients but showed increased CD11c expression by Western blotting compared to the healthy controls. Immunohistochemisty did not show any significant difference between BD patients and classical erythema nosodum.
Discussion: In contrast to previous reports, our study showed decreased CD11a surface expression on peripheral blood. The decrease is likely to have caused from active transmigration of CD11a+ cells in to the tissues, as relative mRNA level and western blot results suggest. CD11c did not show significant difference of surface expression among other groups except the active and the inactive group. Based on the relative low mRNA expression and higher expression in western blot, we suggest that the CD11c might have an immunomodulatory role.
Conclusion: Thus, our study implies that the differential expression of beta 2 integrins in BD patients, especially CD11a and CD11c is likely to be involved in the pathogenesis of BD.Further studies on the functional effect of CD11/CD18 in immunologic abnormalities are needed to elucidate the exact role of CD11/CD18 in BD
ABSTRACT i
TABLE OF CONTENTS iii
LIST OF FIGURES v
LIST OF TABLES vi
INTRODUCTION 1
MATERIAL AND METHODS 4
A. Subjects 4
B. Flow cytometry 4
C. Reverse transcription polymerase chain reaction 5
D. Immunohistochemistry 6
E. Western blot 6
F. Statistical analysis 7
RESULTS 8
A.…
Advisors/Committee Members: 대학원 의학과, 201224102, 박, 영준.
Subjects/Keywords: Beta 2 integrins; Behcet’s disease; flow cytometry; RT-PCR; Western blot
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APA ·
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Manager
APA (6th Edition):
박, . (2014). Differential expression of beta 2 integrins in Korean Behcet’s disease patients. (Thesis). Ajou University. Retrieved from http://repository.ajou.ac.kr/handle/201003/10921 ; http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000017921
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
박, 영준. “Differential expression of beta 2 integrins in Korean Behcet’s disease patients.” 2014. Thesis, Ajou University. Accessed April 13, 2021.
http://repository.ajou.ac.kr/handle/201003/10921 ; http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000017921.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
박, 영준. “Differential expression of beta 2 integrins in Korean Behcet’s disease patients.” 2014. Web. 13 Apr 2021.
Vancouver:
박 . Differential expression of beta 2 integrins in Korean Behcet’s disease patients. [Internet] [Thesis]. Ajou University; 2014. [cited 2021 Apr 13].
Available from: http://repository.ajou.ac.kr/handle/201003/10921 ; http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000017921.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
박 . Differential expression of beta 2 integrins in Korean Behcet’s disease patients. [Thesis]. Ajou University; 2014. Available from: http://repository.ajou.ac.kr/handle/201003/10921 ; http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000017921
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Ottawa
22.
Gandhi, Reno.
Hippocampal Synaptic Plasticity in a Murine Knock-Out Model of Fragile X Syndrome
.
Degree: 2014, University of Ottawa
URL: http://hdl.handle.net/10393/31610
► The dissertation is divided into two separate experiments that explore the effects of visual-spatial learning on PSD-95 dorsal hippocampal expression. Specifically, the aim of these…
(more)
▼ The dissertation is divided into two separate experiments that explore the effects of visual-spatial learning on PSD-95 dorsal hippocampal expression. Specifically, the aim of these studies was to explore the effect of learning an assay, the Hebb-Williams mazes, on the protein expression of PSD-95 in Fmr1 KO mice. PSD-95 is an important scaffolding protein hypothesized to be involved in learning and memory. In cellular models of Fragile X Syndrome it has been shown to be dysregulated but it has never been measured following behavioural learning. Establishment of a deficit using an ecologically valid behavioural assay could lead to the development of novel interventions. Study one employed a subset of the Hebb-Williams mazes of various levels of difficulty to evaluate PSD-95 protein expression in Fmrp intact and Fmr1 KO mice following learning. The results revealed significant increases in PSD-95 protein expression in control runners when compared to Fmr1 KO mice. There was a negative correlation between PSD-95 protein levels and mean total errors on the mazes meaning that as expression was increased, errors were decreased. The goals of study two were to reverse the molecular and behavioural deficits using pharmacological antagonist treatment shown to be effective in cellular models of Fragile X Syndrome. Fmr1 KO mice were treated with either saline or 20 mg/kg of a metabotropic glutamate receptor antagonist, 2-Methyl-6-(phenylethynyl) pyridine (MPEP). Relative to saline treated controls, drug treated Fmr1 KO mice made fewer errors on the same subset of Hebb-Williams mazes used in study one. Latency to complete these mazes did not differ between groups, indicating that MPEP treatment does not adversely affect motor functioning. Protein assessment revealed that PSD-95 was selectively rescued in MPEP treated mice and not saline controls. Similar to study one, a negative correlation between PSD-95 protein levels and mean total errors was observed. When taken together, these studies indicate that protein deficits are associated with a deficit of learning that can be reversed with a selective glutamate receptor antagonist. One of the strengths of the Hebb-Williams mazes is that performance is measurable without floor or ceiling effects, which plague other common behavioural assays. These data further suggest that pharmacological antagonist treatments may be promising in correcting the learning deficits in human Fragile X Syndrome patients.
Subjects/Keywords: Fragile X Syndrome;
Hebb-Williams Mazes;
PSD-95;
Western Blot
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APA (6th Edition):
Gandhi, R. (2014). Hippocampal Synaptic Plasticity in a Murine Knock-Out Model of Fragile X Syndrome
. (Thesis). University of Ottawa. Retrieved from http://hdl.handle.net/10393/31610
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Gandhi, Reno. “Hippocampal Synaptic Plasticity in a Murine Knock-Out Model of Fragile X Syndrome
.” 2014. Thesis, University of Ottawa. Accessed April 13, 2021.
http://hdl.handle.net/10393/31610.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Gandhi, Reno. “Hippocampal Synaptic Plasticity in a Murine Knock-Out Model of Fragile X Syndrome
.” 2014. Web. 13 Apr 2021.
Vancouver:
Gandhi R. Hippocampal Synaptic Plasticity in a Murine Knock-Out Model of Fragile X Syndrome
. [Internet] [Thesis]. University of Ottawa; 2014. [cited 2021 Apr 13].
Available from: http://hdl.handle.net/10393/31610.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Gandhi R. Hippocampal Synaptic Plasticity in a Murine Knock-Out Model of Fragile X Syndrome
. [Thesis]. University of Ottawa; 2014. Available from: http://hdl.handle.net/10393/31610
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Debrecen
23.
Kovács, Elek Gergő.
A humán NOD-like receptor NLRC5 fehérje vizsgálata
.
Degree: DE – Természettudományi és Technológiai Kar – Biológiai és Ökológiai Intézet, 2014, University of Debrecen
URL: http://hdl.handle.net/2437/191934
► A Flag-fúziós fehérjével jelölt NLRC5 fehérjét 293T sejtekben expresszáltattunk. A fúziós fehérje kimutatása során Western-blot eljárásban anti-flag és anti-NLRC5 antitesteket használtunk fel és teszteltünk. Az…
(more)
▼ A Flag-fúziós fehérjével jelölt NLRC5 fehérjét 293T sejtekben expresszáltattunk. A fúziós fehérje kimutatása során
Western-
blot eljárásban anti-flag és anti-NLRC5 antitesteket használtunk fel és teszteltünk. Az anti-flag antitestek használatával sikeresen kimutattuk az overexpresszált fehérjét, ezzel szemben az anti-NLRC5 antitestek kevésbé bizonyultak hatékonynak a flag-tagged NLRC5 fehérje kimutatásában. Az expresszáltatott NLRC5 fehérje jelenlétét
Western-
blot eljárással sikerült kimutatni, az eljárás során anti-flag antitesteket használtunk. Az NLRC5 molekula tömegének meghatározása érdekében teljes sejtlizátumból végeztünk Gélszűrést, az eredmények alapján feltételezhető az overexpresszált NLRC5 oligomerizációja.
Advisors/Committee Members: Mótyán, János András (advisor).
Subjects/Keywords: NLRC5;
Western-blot eljárás;
anti-flag antitest;
expresszálás
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APA ·
Chicago ·
MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Kovács, E. G. (2014). A humán NOD-like receptor NLRC5 fehérje vizsgálata
. (Thesis). University of Debrecen. Retrieved from http://hdl.handle.net/2437/191934
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Kovács, Elek Gergő. “A humán NOD-like receptor NLRC5 fehérje vizsgálata
.” 2014. Thesis, University of Debrecen. Accessed April 13, 2021.
http://hdl.handle.net/2437/191934.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Kovács, Elek Gergő. “A humán NOD-like receptor NLRC5 fehérje vizsgálata
.” 2014. Web. 13 Apr 2021.
Vancouver:
Kovács EG. A humán NOD-like receptor NLRC5 fehérje vizsgálata
. [Internet] [Thesis]. University of Debrecen; 2014. [cited 2021 Apr 13].
Available from: http://hdl.handle.net/2437/191934.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Kovács EG. A humán NOD-like receptor NLRC5 fehérje vizsgálata
. [Thesis]. University of Debrecen; 2014. Available from: http://hdl.handle.net/2437/191934
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Univerzitet u Beogradu
24.
Novaković, Radmila B., 1972-.
Uticaj polifenola prirodnog porekla i sintetskih otvarača
kalijumovih kanala na kontraktilnost izolovanog uterusa : doktorska
disertacija / Radmila B. Novaković.
Degree: Medicinski fakultet, 2015, Univerzitet u Beogradu
URL: https://fedorabg.bg.ac.rs/fedora/get/o:9778/bdef:Content/get
► Medicina - Farmakologija / Medicine - Pharmacology
Prirodni polifenoli su zastupljeni u velikom broju biljnih vrsta. Kao posebni izvori rezveratrola moglo bi se izdvojiti grožđe…
(more)
▼ Medicina - Farmakologija / Medicine -
Pharmacology
Prirodni polifenoli su zastupljeni u velikom broju
biljnih vrsta. Kao posebni izvori rezveratrola moglo bi se
izdvojiti grožđe i vino, kao njegov produkt, a naringenina
grejpfrut, njegov sok, hmelj i pivo. Tokom poslednje decenije
rezveratrol se našao u žiži naučne i šire javnosti kao supstanca
koja usporava starenje, ima antikancerogena, antiinflamatorna,
kardioprotektivna svojstva. Kao moguća mesta dejstva pokazan je
veliki broj ćelijskih struktura, stoga je rezveratrol označen kao
"jedan molekul - mnogo meta". Između ostalog, pokazano je da
rezveratrol dovodi do relaksacije glatkih mišića mnogobrojnih
krvnih sudova kao i glatkih mišića žučne kese. Kalijumovi kanali su
direktno ili indirektno ukljućeni u mehanizam dejstva rezveratrola.
Za razliku od njega, naringenin, pripada manje proučavanoj grupi,
flavonoidima. Njegov mehanizam inhibicije kontrakcija glatkih
mišića uterusa još uvek nije proučen. Pored velikog interesovanja
za rezveratrol, njegov uticaj na kontraktilnost glatke muskulature
uterusa nije izučavan. Stoga su ciljevi ove studije bili da
ispitimo moguće inhibitorno dejstvo rezveratrola i naringenina na
nekoliko eksperimentalnih modela negravidnog i gravidnog uterusa i
da definišemo ulogu kalijumovih kanala u tim mehanizmima dejstva.
Nepoželjna kontraktilnost uterusa je uzrok poremećaja kao što su
dismenoreja, kod negravidnog, a prevremeni porođaj, kod gravidnog
uterusa. Do danas, ni jedan od ovih problema nije farmakološki
rešen. Dismenoreja se uglavnom tretira nesteroidnim
antiinflamatornim lekovima, koji nisu uspešni kod svih pacijenata i
imaju izrazite neželjene efekte. Dodatno, prevremeni porođaj je
uzrok morbiditeta novorođenčati u 50 % slučajeva. Malo je dokaza da
lekovi koji se koriste za suzbijanje prevremenih kontrakcija zaista
efikasni. Prevremeni porođaj je češći i ozbiljniji problem u grupi
pacijenkinja iz progrma in vitro oplodnje. Shodno tome, idealno
sredstvo za prevenciju i lečenje neželjene kontraktilnosti uterusa
još uvek nije pronađen...
Advisors/Committee Members: Gojković Bukarica, Ljiljana, 1962-.
Subjects/Keywords: uterus; myometrium; contractions; potassium channels;
resveratrol; naringenin; immunohistochemical staining; western
blot
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Novaković, Radmila B., 1. (2015). Uticaj polifenola prirodnog porekla i sintetskih otvarača
kalijumovih kanala na kontraktilnost izolovanog uterusa : doktorska
disertacija / Radmila B. Novaković. (Thesis). Univerzitet u Beogradu. Retrieved from https://fedorabg.bg.ac.rs/fedora/get/o:9778/bdef:Content/get
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Novaković, Radmila B., 1972-. “Uticaj polifenola prirodnog porekla i sintetskih otvarača
kalijumovih kanala na kontraktilnost izolovanog uterusa : doktorska
disertacija / Radmila B. Novaković.” 2015. Thesis, Univerzitet u Beogradu. Accessed April 13, 2021.
https://fedorabg.bg.ac.rs/fedora/get/o:9778/bdef:Content/get.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Novaković, Radmila B., 1972-. “Uticaj polifenola prirodnog porekla i sintetskih otvarača
kalijumovih kanala na kontraktilnost izolovanog uterusa : doktorska
disertacija / Radmila B. Novaković.” 2015. Web. 13 Apr 2021.
Vancouver:
Novaković, Radmila B. 1. Uticaj polifenola prirodnog porekla i sintetskih otvarača
kalijumovih kanala na kontraktilnost izolovanog uterusa : doktorska
disertacija / Radmila B. Novaković. [Internet] [Thesis]. Univerzitet u Beogradu; 2015. [cited 2021 Apr 13].
Available from: https://fedorabg.bg.ac.rs/fedora/get/o:9778/bdef:Content/get.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Novaković, Radmila B. 1. Uticaj polifenola prirodnog porekla i sintetskih otvarača
kalijumovih kanala na kontraktilnost izolovanog uterusa : doktorska
disertacija / Radmila B. Novaković. [Thesis]. Univerzitet u Beogradu; 2015. Available from: https://fedorabg.bg.ac.rs/fedora/get/o:9778/bdef:Content/get
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
25.
Österberg, Emmy.
Ro52 : Structure and interactions of constructs of RING and B-box.
Degree: The Institute of Technology, 2014, Linköping UniversityLinköping University
URL: http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-110362
► The ubiquitination process is vital to maintain the protein homeostasis in the cell. With high specificity it regulates degradation of proteins by tagging them…
(more)
▼ The ubiquitination process is vital to maintain the protein homeostasis in the cell. With high specificity it regulates degradation of proteins by tagging them with a small protein called ubiquitin. Four proteins are involved to perform the process and in this thesis one of these proteins is studied. This protein is called Ro52 and belongs to the TRIM protein family. It posses E3 ligase activity because of a N-terminal RING-domain and therefore it is responsible for the last step in the ubiquitination process. The structure of Ro52 is not totally solved and the function of the protein’s four domains is not fully understood. In this thesis three constructs of two domains from Ro52 (RING and B-box) is investigated by circular dichroism (CD), nuclear magnetic resonance (NMR) spectroscopy and auto-ubiquitination assay by Western blot. The goal was to gain deeper insight in structural and functional properties of these domains. In the end only two constructs were investigated because of time limitations. It was shown by NMR that one construct has similar structure as the wild type but lower stability, possibly due to shorter N-terminal region. Comparison of the results from CD measurements showed that the constructs were well structured but did not reveal any significant differences in secondary structure between the constructs. Functional analysis by Western blot encountered unexpected problems and no results were obtained. The current thesis provides a basis for further investigation of variant constructs jointly expressing the RING-B-box domains, and shows that even small changes may alter structure and stability in ways that might affect functional properties.
Subjects/Keywords: Structural biology; Ro52; TRIM21; Ubiquitination; Western blot; Protein expression; Protein purification
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APA ·
Chicago ·
MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Österberg, E. (2014). Ro52 : Structure and interactions of constructs of RING and B-box. (Thesis). Linköping UniversityLinköping University. Retrieved from http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-110362
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Österberg, Emmy. “Ro52 : Structure and interactions of constructs of RING and B-box.” 2014. Thesis, Linköping UniversityLinköping University. Accessed April 13, 2021.
http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-110362.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Österberg, Emmy. “Ro52 : Structure and interactions of constructs of RING and B-box.” 2014. Web. 13 Apr 2021.
Vancouver:
Österberg E. Ro52 : Structure and interactions of constructs of RING and B-box. [Internet] [Thesis]. Linköping UniversityLinköping University; 2014. [cited 2021 Apr 13].
Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-110362.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Österberg E. Ro52 : Structure and interactions of constructs of RING and B-box. [Thesis]. Linköping UniversityLinköping University; 2014. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-110362
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
IUPUI
26.
TruongVo, ThucNhi.
Application of pulse width modulation to a Western blotting device.
Degree: 2016, IUPUI
URL: http://hdl.handle.net/1805/11824
► Indiana University-Purdue University Indianapolis (IUPUI)
One of the critical steps in a current Western blot technique is a blotting process, which in general requires one…
(more)
▼ Indiana University-Purdue University Indianapolis (IUPUI)
One of the critical steps in a current Western blot technique is a blotting process, which in general requires one electrophoretic gel for every protein species to be analyzed. In most cases, multiple protein species are analyzed simultaneously and thus it is necessary for a scientist to run multiple gels. In order to make it possible to analyze multiple protein species from a single gel, a novel blotting device, BlotMan, was employed in this study. Designed by Dr. Chien’s group (YC Bioelectric), BlotMan uses pulse width modulation (PWM) for applying a protein size-dependent voltage during a blotting process. In this study, the differential average voltage profile, depending on protein size (e.g. 17 kDa to 140 kDa), was built and enabled BlotMan to transfer all protein species in equal efficiency regardless of the protein size. Furthermore, Blot- Man consists of a user-friendly, custom-made interface box, which can be remotely controlled by a smart phone. BlotMan’s capability was evaluated using standard protein markers, as well as protein samples that were isolated from chondrosarcoma cells (SW1353) and breast cancer cells (MDA-MB-213). The experimental results revealed that BlotMan was capable of generating 5 blotting membranes from a single gel simultaneously. Protein species such as c-Src, eukaryotic translation initiation factor 2 alpha (eIF2α) and its phosphorylated form (p-eIF2α), lamin B, and β-actin were successfully detected. It is also demonstrated that compared to a regular constant voltage, PWM signals improved transfer efficiency and a signal-to-noise ratio. In conclusion, this study demonstrated that BlotMan was able to facilitate Western blotting analysis by generating multiple blotting membranes from a single gel with an improved signal-to-noise ratio. Further analysis is recommended for understanding the mechanism of PWMts action on transfer efficiency and noise reduction.
Advisors/Committee Members: Yokota, Hiroki.
Subjects/Keywords: Pulse width modulation; protein transfer process; Western blot; BlotMan
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APA (6th Edition):
TruongVo, T. (2016). Application of pulse width modulation to a Western blotting device. (Thesis). IUPUI. Retrieved from http://hdl.handle.net/1805/11824
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
TruongVo, ThucNhi. “Application of pulse width modulation to a Western blotting device.” 2016. Thesis, IUPUI. Accessed April 13, 2021.
http://hdl.handle.net/1805/11824.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
TruongVo, ThucNhi. “Application of pulse width modulation to a Western blotting device.” 2016. Web. 13 Apr 2021.
Vancouver:
TruongVo T. Application of pulse width modulation to a Western blotting device. [Internet] [Thesis]. IUPUI; 2016. [cited 2021 Apr 13].
Available from: http://hdl.handle.net/1805/11824.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
TruongVo T. Application of pulse width modulation to a Western blotting device. [Thesis]. IUPUI; 2016. Available from: http://hdl.handle.net/1805/11824
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Melbourne
27.
Parkin, Georgia May.
Catechol metabolism and glutamate uptake in the prefrontal cortex of subjects with schizophrenia: implications for cognitive function.
Degree: 2019, University of Melbourne
URL: http://hdl.handle.net/11343/224293
► My thesis comprises of two projects, the first which investigates catechol-O-methyltransferase (COMT) protein levels by genotype (chapter 1 and 2), and the second which investigates…
(more)
▼ My thesis comprises of two projects, the first which investigates catechol-O-methyltransferase (COMT) protein levels by genotype (chapter 1 and 2), and the second which investigates excitatory amino acid transporter (EAAT)1 and EAAT2 and metabotropic glutamate receptor 5 mRNA levels (chapter 3) in the prefrontal cortex of subjects with schizophrenia.
Project 1. COMT genotype has previously been associated with cognitive function; I hypothesized that COMT genotype would also be associated with differing levels of cortical COMT protein. Using
western blotting, I measured protein levels of the two COMT protein isoforms, membrane bound (MB-) COMT and soluble (S-) COMT, as well as beta-actin protein levels, in Brodmann’s area (BA) 9 of 199 individuals, 119 of whom had psychiatric disorders. I have shown that levels of S-COMT protein vary with genotype at rs4818 and rs4680, but not rs737865 and rs165599 (rs4818 genotype: CC<CG, p=0.03, CC<GG, p=0.002; rs4680 genotype: AA<GG, p=0.001), and that levels of MB-COMT protein vary with genotype at rs165599 (GG<AG, p=0.02, GG<AA, p=0.04). As S-COMT is located in the cytosol where it is unlikely to have access to dopamine, my results contest the belief that the associations between rs4680, rs4818 and cognition are through COMT-mediated dopamine degradation. Instead, I propose that S-COMT protein modulates cognition through metabolism of other catechol structures, such as catecholestrogens. In support of this, an Affymetrix™ microarray analysis has found that the cortical expression of 15 genes vary by COMT genotype at rs4680 and/or rs4818, with 11 of these genes previously shown to be regulated by estrogen. COMT protein levels were not affected by a diagnosis of schizophrenia (p>0.05), suggesting that this variation in protein level by genotype occurs even in the presence of cortical dysfunction.
Project 2. EAAT1 and EAAT2 mediate glutamatergic neurotransmission and prevent glutamate excitotoxicity, through the perisynaptic binding and transportation of glutamate into glia. An Affymetrix™ microarray analysis which preceded my PhD studies found a 36% increase in EAAT1 mRNA levels in BA9 of subjects with schizophrenia. The aim of this chapter was to determine whether changes in EAAT1 and EAAT2 mRNA levels occur in other cortical regions from subjects with schizophrenia. I used quantitative PCR to compare mRNA levels of EAAT1, EAAT2 and mGluR5 across post-mortem BA10, BA44 and BA46 of subjects with schizophrenia (n=20) and non-psychiatric controls (n=18). Reactions were measured in triplicate with results normalised to the geometric mean of two stably expressed reference genes – transcription factor B1, mitochondrial (TFB1M) and S-phase kinase-associated protein 1A (SKP1A). EAAT1 mRNA levels were significantly higher in BA10 (U=67, p=0.0006) and BA44 (U=68, p=0.0007), and EAAT2 mRNA levels w¬ere significantly higher in BA10 (U=85, p=0.0047), with mRNA levels of both transporters showing no change in BA46 (EAAT1: U=164, p=0.65; EAAT2: U=146, p=0.33), of subjects with…
Subjects/Keywords: post-mortem; glutamate; catechol-O-methyltransferase; schizophrenia; western blot; qPCR
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APA ·
Chicago ·
MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Parkin, G. M. (2019). Catechol metabolism and glutamate uptake in the prefrontal cortex of subjects with schizophrenia: implications for cognitive function. (Doctoral Dissertation). University of Melbourne. Retrieved from http://hdl.handle.net/11343/224293
Chicago Manual of Style (16th Edition):
Parkin, Georgia May. “Catechol metabolism and glutamate uptake in the prefrontal cortex of subjects with schizophrenia: implications for cognitive function.” 2019. Doctoral Dissertation, University of Melbourne. Accessed April 13, 2021.
http://hdl.handle.net/11343/224293.
MLA Handbook (7th Edition):
Parkin, Georgia May. “Catechol metabolism and glutamate uptake in the prefrontal cortex of subjects with schizophrenia: implications for cognitive function.” 2019. Web. 13 Apr 2021.
Vancouver:
Parkin GM. Catechol metabolism and glutamate uptake in the prefrontal cortex of subjects with schizophrenia: implications for cognitive function. [Internet] [Doctoral dissertation]. University of Melbourne; 2019. [cited 2021 Apr 13].
Available from: http://hdl.handle.net/11343/224293.
Council of Science Editors:
Parkin GM. Catechol metabolism and glutamate uptake in the prefrontal cortex of subjects with schizophrenia: implications for cognitive function. [Doctoral Dissertation]. University of Melbourne; 2019. Available from: http://hdl.handle.net/11343/224293
28.
Justis, Brooke.
Levels of human tear lacritin isoforms in healthy adults.
Degree: 2019, James Madison University
URL: https://commons.lib.jmu.edu/honors201019/687
► Current diagnostics of dry eye disease are unable to identify the multiple complex causes of dry eye, thereby limiting the proper treatments of the disease.…
(more)
▼ Current diagnostics of dry eye disease are unable to identify the multiple complex causes of dry eye, thereby limiting the proper treatments of the disease. The tear glycoprotein lacritin has been reported to be decreased in dry eye patients, specifically those with Sjӧgren’s syndrome associated dry eye. Therefore, the use of lacritin in a diagnostic test for dry eye could improve the current diagnostics available.
Western blot assays have revealed the presence of an active lacritin monomer as well as an inactive tissue transglutaminase cross-linked polymer in human tears. A third isoform of lacritin, the splice variant lacritin-c, has also been detected in human tears. Using a diagnostic multiplex
western blot assay, the three lacritin isoforms were quantitated in forty samples of human tears from twenty adults and analyzed using JMP software. This provided a baseline distribution of percent lacritin in human tears that will be used in future lacritin clinical trials.
Advisors/Committee Members: Robert L. McKown.
Subjects/Keywords: Lacritin; Isoform; Western Blot; Immunoassay; Diagnostic; Tear; Ophthalmology
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APA ·
Chicago ·
MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Justis, B. (2019). Levels of human tear lacritin isoforms in healthy adults. (Masters Thesis). James Madison University. Retrieved from https://commons.lib.jmu.edu/honors201019/687
Chicago Manual of Style (16th Edition):
Justis, Brooke. “Levels of human tear lacritin isoforms in healthy adults.” 2019. Masters Thesis, James Madison University. Accessed April 13, 2021.
https://commons.lib.jmu.edu/honors201019/687.
MLA Handbook (7th Edition):
Justis, Brooke. “Levels of human tear lacritin isoforms in healthy adults.” 2019. Web. 13 Apr 2021.
Vancouver:
Justis B. Levels of human tear lacritin isoforms in healthy adults. [Internet] [Masters thesis]. James Madison University; 2019. [cited 2021 Apr 13].
Available from: https://commons.lib.jmu.edu/honors201019/687.
Council of Science Editors:
Justis B. Levels of human tear lacritin isoforms in healthy adults. [Masters Thesis]. James Madison University; 2019. Available from: https://commons.lib.jmu.edu/honors201019/687
29.
Cunha, Ana Isabel Ribeiro da.
Estudo da expressão da proteína LRP1B em tumores de ovário
.
Degree: 2019, Universidade de Aveiro
URL: http://hdl.handle.net/10773/30172
► O LRP1B (Recetor de lipoproteína de baixa densidade ligado à proteína 1B), pertencente à superfamília de recetores de lipoproteína de baixa densidade, interage com vários…
(more)
▼ O LRP1B (Recetor de lipoproteína de baixa densidade ligado à proteína 1B), pertencente à superfamília de recetores de lipoproteína de baixa densidade, interage com vários ligandos e serve como mediador da endocitose. O seu gene está descrito como sendo um dos 10 genes mais deletados em cancro. Embora o papel do LRP1B no cancro não seja ainda totalmente claro, pensa-se que a sua atividade endocítica possa desempenhar um papel importante na modulação da disponibilidade de fatores no microambiente tumoral. Pode ainda intervir na internalização de fármacos e assim contribuir para a resistência de terapias lipossomais, como por exemplo à doxorrubicina lipossomal em cancro de ovário. A maioria dos estudos sobre a disfunção do LRP1B têm por base a análise de DNA/RNA. Embora haja alguns estudos que referem a utilização de anticorpos para a deteção da proteína LRP1B, a informação é ainda escassa, provavelmente devido à pouca informação sobre os anticorpos utilizados e ao facto do LRP1B ser uma proteína de elevado peso molecular, codificada por um cDNA longo difícil de clonar. Nos últimos anos, novos anticorpos para o LRP1B têm começado a aparecer o que poderá ajudar na melhor caracterização da função desta proteína em cancro.
Assim, este projeto teve como objetivo principal a análise da expressão e localização de LRP1B em carcinoma de ovário com recurso a anticorpos comerciais, usando as metodologias de
Western blot e imunohistoquímica (IHQ). Pretendeu-se ainda com este trabalho adquirir competências em cultura celular de linhas celulares tumorais humanas e em metodologias de análise da resposta a fármacos usando, como modelo, linhas celulares de carcinoma de ovário com sobre-expressão de LRP1B.
Se por um lado, não foi possível avaliar a expressão de LRP1B em linhas celulares tumorais de ovário por
Western blot (devido à inespecificidade dos anticorpos usados nas condições testadas) por outro lado, os resultados obtidos com a metodologia de IHQ foram bastante positivos. De facto, foi possível não só a otimização dos anticorpos como também o seu uso na análise da expressão do LRP1B em cancro de ovário. Foi analisada uma série de amostras de carcinoma de ovário obtidas de pacientes sujeitas a tratamento com quimioterapia que incluía dois grupos: um de pacientes tratadas com doxorrubicina lipossomal (“tratadas com DL”) e outro de pacientes tratadas com outras quimioterapias (“não tratadas com DL”). O padrão de marcação LRP1B observado era maioritariamente citoplasmático e, em alguns casos, membranar. Uma análise semi-quantitativa dos casos permitiu fazer uma avaliar relativamente à intensidade da marcação de LRP1B (ausente, fraca, moderada e forte) e à percentagem de células tumorais marcadas positivamente (< 5%; 5-25%; 25-50%, 50-75% e > 75%). Não houve diferenças significativas na expressão do LRP1B entre os casos e os controlos.
Por último, foi analisada a resposta de uma linha de carcinoma de ovário com sobre-expressão de LRP1B à doxorrubicina lipossomal, tendo-se verificado uma tendência de aumento da sensibilidade a…
Advisors/Committee Members: Lima, Raquel (advisor), Mendo, Sónia (advisor).
Subjects/Keywords: LRP1B;
Cancro;
Imunohistoquímica;
Western blot;
Anticorpos;
Tumores de ovário
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APA (6th Edition):
Cunha, A. I. R. d. (2019). Estudo da expressão da proteína LRP1B em tumores de ovário
. (Thesis). Universidade de Aveiro. Retrieved from http://hdl.handle.net/10773/30172
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Cunha, Ana Isabel Ribeiro da. “Estudo da expressão da proteína LRP1B em tumores de ovário
.” 2019. Thesis, Universidade de Aveiro. Accessed April 13, 2021.
http://hdl.handle.net/10773/30172.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Cunha, Ana Isabel Ribeiro da. “Estudo da expressão da proteína LRP1B em tumores de ovário
.” 2019. Web. 13 Apr 2021.
Vancouver:
Cunha AIRd. Estudo da expressão da proteína LRP1B em tumores de ovário
. [Internet] [Thesis]. Universidade de Aveiro; 2019. [cited 2021 Apr 13].
Available from: http://hdl.handle.net/10773/30172.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Cunha AIRd. Estudo da expressão da proteína LRP1B em tumores de ovário
. [Thesis]. Universidade de Aveiro; 2019. Available from: http://hdl.handle.net/10773/30172
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
30.
PASSOS, G. P.
Avaliação Soro-epidemiológica e Molecular de Cães Assintomática para Leishmaniose Tegumentar Americana em Área Endêmica.
Degree: 2013, Universidade Federal do Espírito Santo; Mestrado em Ciências Veterinárias; Programa de Pós-Graduação em Ciências Veterinárias; UFES; BR
URL: http://repositorio.ufes.br/handle/10/7725
► Made available in DSpace on 2018-08-01T22:56:45Z (GMT). No. of bitstreams: 1 tese_6434_DISSERTAÇÃO GABRIELA PORFIRIO-PASSOS.pdf: 872492 bytes, checksum: 4693b33b1f6f17af259a9c2b1b3878cb (MD5) Previous issue date: 2013-02-26
Com o…
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▼ Made available in DSpace on 2018-08-01T22:56:45Z (GMT). No. of bitstreams: 1 tese_6434_DISSERTAÇÃO GABRIELA PORFIRIO-PASSOS.pdf: 872492 bytes, checksum: 4693b33b1f6f17af259a9c2b1b3878cb (MD5) Previous issue date: 2013-02-26
Com o objetivo de realizar o diagnóstico de leishmaniose tegumentar americana (LTA) em cães, foram utilizados métodos de cultura e isolamento, testes sorológicos de ELISA e Western Blot (WB) e pesquisa de DNA do parasito para dois grupos de animais, um grupo composto de animais sem lesões clínicas sugestivas de LTA, mas residentes em torno de casos clínicos humanos confirmados para LTA, e outro grupo de animais com lesões sugestivas de LTA que serviram como controle dos protocolos realizados. O estudo foi realizado no município de Iúna, ES, Brasil, região endêmica para enfermidade. No primeiro grupo, foram analisadas amostras de soro de 109 animais sem histórico ou lesões indicativas de LTA, estas foram submetidas às técnicas
de ELISA e WB que resultaram em 20 animais sorologicamente positivos para as duas técnicas. O teste ELISA apresentou sensibilidade de 100,00% (IC95% - 0,83 a 1,00) e especificidade de 77,53% (IC95% - 0,67 a 0,86), em relação à técnica de WB. O teste WB apresentou maior acurácia e mostrou-se mais adequado para diagnóstico dos animais assintomáticos, enquanto a técnica de ELISA para a triagem. Para a pesquisa do DNA do parasito nos 20 animais assintomáticos e positivos pela técnica de WB utilizou-se sangue total e biópsia de tecido íntegro do pavilhão auricular pela técnica de reação em cadeia da polimerase (PCR). Os resultados para PCR da biópsia de tecido íntegro e PCR de tecido sanguíneo mostraram sensibilidade de 30,0% (IC95% - 0,12 a 0,54) e 20,0% (IC95% - 0,06 a 0,44), respectivamente. A especificidade, 99,0% (IC95% - 0,93 a 0,99) e 100% (IC95% - 0,96 a 1,00), para a PCR da biópsia de tecido íntegro e PCR de tecido sanguíneo quando comparadas ao WB. Os resultados mostram que
tecido íntegro do pavilhão auricular e sangue de animais assintomáticos submetidos à técnica de PCR, apresentaram baixa sensibilidade e alta especificidade, assim, a PCR da biópsia de tecido íntegro é melhor indicador para animais assintomáticos que a PCR de tecido sanguíneo. Para o segundo grupo, foram identificados três animais com lesões sugestivas para LTA e sorologicamente positivo. A partir de uma amostra de biópsia de lesão sugestiva de LTA presente no pavilhão auricular, parte desta foi destinada a cultura e isolamento e outra parte para comparação de três protocolos de extração do DNA de tecido animal para diagnóstico da LTA canina. Os protocolos utilizados tiveram como base o Fenol-Clorofórmio, Acetato de Potássio e associação entre as duas metodologias. Em comparação com os padrões moleculares de concentração de DNA concluiu-se que o protocolo com Acetato de Potássio foi o mais indicado para o tipo de tecido empregado. Por fim, de posse dos dados apresentados para os
animais sorologicamente positivos e assintomáticos estudados, foi possível concluir que estes não representaram…
Advisors/Committee Members: MARTINS, I. V. F., ZANINI, M. S..
Subjects/Keywords: Extração de DNA; ELISA; Western Blot; PCR; Leishmania brazil
Record Details
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Record Details
Similar Records
Cite
« Share
❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
PASSOS, G. P. (2013). Avaliação Soro-epidemiológica e Molecular de Cães Assintomática para Leishmaniose Tegumentar Americana em Área Endêmica. (Masters Thesis). Universidade Federal do Espírito Santo; Mestrado em Ciências Veterinárias; Programa de Pós-Graduação em Ciências Veterinárias; UFES; BR. Retrieved from http://repositorio.ufes.br/handle/10/7725
Chicago Manual of Style (16th Edition):
PASSOS, G P. “Avaliação Soro-epidemiológica e Molecular de Cães Assintomática para Leishmaniose Tegumentar Americana em Área Endêmica.” 2013. Masters Thesis, Universidade Federal do Espírito Santo; Mestrado em Ciências Veterinárias; Programa de Pós-Graduação em Ciências Veterinárias; UFES; BR. Accessed April 13, 2021.
http://repositorio.ufes.br/handle/10/7725.
MLA Handbook (7th Edition):
PASSOS, G P. “Avaliação Soro-epidemiológica e Molecular de Cães Assintomática para Leishmaniose Tegumentar Americana em Área Endêmica.” 2013. Web. 13 Apr 2021.
Vancouver:
PASSOS GP. Avaliação Soro-epidemiológica e Molecular de Cães Assintomática para Leishmaniose Tegumentar Americana em Área Endêmica. [Internet] [Masters thesis]. Universidade Federal do Espírito Santo; Mestrado em Ciências Veterinárias; Programa de Pós-Graduação em Ciências Veterinárias; UFES; BR; 2013. [cited 2021 Apr 13].
Available from: http://repositorio.ufes.br/handle/10/7725.
Council of Science Editors:
PASSOS GP. Avaliação Soro-epidemiológica e Molecular de Cães Assintomática para Leishmaniose Tegumentar Americana em Área Endêmica. [Masters Thesis]. Universidade Federal do Espírito Santo; Mestrado em Ciências Veterinárias; Programa de Pós-Graduação em Ciências Veterinárias; UFES; BR; 2013. Available from: http://repositorio.ufes.br/handle/10/7725
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