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You searched for subject:(Viral GPCR). Showing records 1 – 3 of 3 total matches.

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1. Bittencourt, Fabiola M. Examination of the Function of the Murine Cytomegalovirus Encoded G Protein-Coupled Receptor M33 in vivo.

Degree: PhD, Medicine: Molecular Genetics, Biochemistry, and Microbiology, 2014, University of Cincinnati

The betaherpesvirus Human Cytomegalovirus (HCMV) is estimated to be present in 40-80% of world population. HCMV infection in a healthy person causes mild symptoms, although the virus persists in the host in the latent form. Immunocompromised patients such as HIV and organ transplant patients as well as fetuses and neonatal babies with underdeveloped immune system are most affected by HCMV infection. CMVs are characterized by their strict species specifity; therefore humans are the only host for HCMV. The mouse cytomegalovirus (MCMV) has been the most useful animal model to explore HCMV spread and disease. CMVs encode G Protein-Coupled Receptors (GPCR) and these viral GPCRs are increasingly recognized as important regulators of pathogenesis and disease. HCMV for example encodes four of these GPCRs, whereas MCMV expresses two: M33 and M78. M33 shows constitutive signaling properties and activates various signaling pathways. M33 stimulates the PLC-&beta/PKC pathway via G&alphaq/11, however activation of NF-&kappaB and p38 MAP-kinase is independent of G-proteins. Importantly, M33 is essential for dissemination to or growth in salivary glands of immunocompetent mice and the G-protein signaling ability of M33 is necessary for viral growth at this site. Here we used the non-obese diabetic (NOD) and the immunocompromised NOD scid gamma (NSG) mouse models to assess a potential role for M33 as an immunomodulator and to further investigate the mechanism(s) by which M33 promotes viral growth in vivo. We are able to detect replication of &DeltaM33 viruses in the salivary glands of immunocompromised NSG mice; however it exhibited a 400x defect compared to wildtype MCMV. Since the growth phenotype is not completely reverted in the NSG mice, we conclude that M33 is not solely functioning to modulate the immune system. We also showed that M33 is dispensable for hematogenous dissemination within the host and that activation of G&alphaq signaling pathways alone is not sufficient to promote salivary gland growth as neither HCMV US28 nor a constitutively active form of G&alphaq are able to complement the unique functions of M33. We also describe here the generation and characterization of an in vitro cell culture model with cells extracted from mouse salivary glands. Specialized growth conditions resulted in cells that express markers of salivary gland epithelium, but none resulted in maintenance of epithelial markers similar to those expressed by the organ in vivo. Despite growth conditions that enable sustained expression of at least low levels of the salivary epithelial markers, M33 was not required for viral growth in these primary cells. Taken together, these studies suggest that M33 is necessary to allow viral growth within the three-dimensional structure of the gland in vivo but that this activity is not required once the structure of the gland is disrupted. An understanding of CMV replication in the salivary gland and how M33 modulates salivary gland function to promote viral replication will provide insights into mechanisms that… Advisors/Committee Members: Miller, William (Committee Chair).

Subjects/Keywords: Virology; Murine Cytomegalovirus; Viral GPCR; M33

…of the desensitization process in regulating viral GPCR function in vivo as assessed in… …the roles of the MCMV encoded GPCR M33 and M33 signaling, in viral replication in salivary… …dissemination. Among these viral receptors, the MCMV encoded M33 GPCR is an activator of CREB, NF-κB… …GPCR) Signaling Schematic. Table 1: GPCRs encoded by herpesviruses. Chapter II: Table 2… …Primers used for PCR analysis at the Gq and G11 loci. Figure 2: M33 is required for viral… 

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Bittencourt, F. M. (2014). Examination of the Function of the Murine Cytomegalovirus Encoded G Protein-Coupled Receptor M33 in vivo. (Doctoral Dissertation). University of Cincinnati. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=ucin1397234044

Chicago Manual of Style (16th Edition):

Bittencourt, Fabiola M. “Examination of the Function of the Murine Cytomegalovirus Encoded G Protein-Coupled Receptor M33 in vivo.” 2014. Doctoral Dissertation, University of Cincinnati. Accessed December 09, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1397234044.

MLA Handbook (7th Edition):

Bittencourt, Fabiola M. “Examination of the Function of the Murine Cytomegalovirus Encoded G Protein-Coupled Receptor M33 in vivo.” 2014. Web. 09 Dec 2019.

Vancouver:

Bittencourt FM. Examination of the Function of the Murine Cytomegalovirus Encoded G Protein-Coupled Receptor M33 in vivo. [Internet] [Doctoral dissertation]. University of Cincinnati; 2014. [cited 2019 Dec 09]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=ucin1397234044.

Council of Science Editors:

Bittencourt FM. Examination of the Function of the Murine Cytomegalovirus Encoded G Protein-Coupled Receptor M33 in vivo. [Doctoral Dissertation]. University of Cincinnati; 2014. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=ucin1397234044

2. Westen, Gerard Jacob Pieter van. Déjà Vu - Réjà Vu: on knowledge-based approaches linking ligand and target information to bioactivity.

Degree: 2013, Department of Medicinal Chemistry, Leiden/Amsterdam Center for Drug Research (LACDR), Faculty of Science, Leiden University

Over the last decades several disciplines relevant to medicinal chemistry and preclinical drug discovery have made gigantic leaps; this includes chemistry, biology and measurement of bioactivity. Better techniques have led to massive amounts of data. Moreover, sources of chemical and bioactivity data have become available in the public domain. Hence there is a need for new techniques combining and mining these data sources. This thesis focuses on computational methods combining data from these disciplines and demonstrates that the sum of these methods leads to better quality predictions than models using the individual data sources. One of the techniques central in this thesis is proteochemometric modeling, a machine learning approach linking chemical descriptors and protein descriptors to a biologically relevant output variable. This output variable describes the activity of molecules on biological macromolecules and hence proteochemometric models can make relevant predictions for both unseen molecules and unseen macromolecules (e.g. novel viral mutants). Secondly we present a novel technique that is able to combine information from multiple crystal structures in such a way that shared and unique pharmacophoric features can be isolated and visualized. Approaches presented here have been validated prospectively and have been shown to be widely applicable.

Subjects/Keywords: Proteochemometrics; Machine learning; Polypharmacology; Viral resistance; Hiv; GPCR; QSAR; Consensus structures; Chemogenomics; Cheminformatics; Ortholog; Paralog; Proteochemometrics; Machine learning; Polypharmacology; Viral resistance; Hiv; GPCR; QSAR; Consensus structures; Chemogenomics; Cheminformatics; Ortholog; Paralog

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Westen, G. J. P. v. (2013). Déjà Vu - Réjà Vu: on knowledge-based approaches linking ligand and target information to bioactivity. (Doctoral Dissertation). Department of Medicinal Chemistry, Leiden/Amsterdam Center for Drug Research (LACDR), Faculty of Science, Leiden University. Retrieved from http://hdl.handle.net/1887/20394

Chicago Manual of Style (16th Edition):

Westen, Gerard Jacob Pieter van. “Déjà Vu - Réjà Vu: on knowledge-based approaches linking ligand and target information to bioactivity.” 2013. Doctoral Dissertation, Department of Medicinal Chemistry, Leiden/Amsterdam Center for Drug Research (LACDR), Faculty of Science, Leiden University. Accessed December 09, 2019. http://hdl.handle.net/1887/20394.

MLA Handbook (7th Edition):

Westen, Gerard Jacob Pieter van. “Déjà Vu - Réjà Vu: on knowledge-based approaches linking ligand and target information to bioactivity.” 2013. Web. 09 Dec 2019.

Vancouver:

Westen GJPv. Déjà Vu - Réjà Vu: on knowledge-based approaches linking ligand and target information to bioactivity. [Internet] [Doctoral dissertation]. Department of Medicinal Chemistry, Leiden/Amsterdam Center for Drug Research (LACDR), Faculty of Science, Leiden University; 2013. [cited 2019 Dec 09]. Available from: http://hdl.handle.net/1887/20394.

Council of Science Editors:

Westen GJPv. Déjà Vu - Réjà Vu: on knowledge-based approaches linking ligand and target information to bioactivity. [Doctoral Dissertation]. Department of Medicinal Chemistry, Leiden/Amsterdam Center for Drug Research (LACDR), Faculty of Science, Leiden University; 2013. Available from: http://hdl.handle.net/1887/20394


University of Queensland

3. Trindade Oliveira, Martha. Cell-type specific activities of cytomegalovirus GPCR homologues.

Degree: School of Chemistry & Molecular Biosciences, 2016, University of Queensland

Subjects/Keywords: cytomegalovirus; viral GPCR; migration; chemokines; trophoblast; endothelial cells; intranasal dissemination; footpad dissemination; LysM; CD11c; 0605 Microbiology; 110804 Medical Virology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Trindade Oliveira, M. (2016). Cell-type specific activities of cytomegalovirus GPCR homologues. (Thesis). University of Queensland. Retrieved from http://espace.library.uq.edu.au/view/UQ:406757

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Trindade Oliveira, Martha. “Cell-type specific activities of cytomegalovirus GPCR homologues.” 2016. Thesis, University of Queensland. Accessed December 09, 2019. http://espace.library.uq.edu.au/view/UQ:406757.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Trindade Oliveira, Martha. “Cell-type specific activities of cytomegalovirus GPCR homologues.” 2016. Web. 09 Dec 2019.

Vancouver:

Trindade Oliveira M. Cell-type specific activities of cytomegalovirus GPCR homologues. [Internet] [Thesis]. University of Queensland; 2016. [cited 2019 Dec 09]. Available from: http://espace.library.uq.edu.au/view/UQ:406757.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Trindade Oliveira M. Cell-type specific activities of cytomegalovirus GPCR homologues. [Thesis]. University of Queensland; 2016. Available from: http://espace.library.uq.edu.au/view/UQ:406757

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

.