subject:(Vascular grafts)

University of Utah

1. Vickers, Daniel Baker. Hyperthermia for treatment of hyperplasia in a hemodialysis access graft.

Degree: MS;, Bioengineering;, 2008, University of Utah

URL: http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/1473/rec/598

► I would like to express my appreciation to my supervisory committee. Committee chair Dr. Douglas Christensen provided the knowledge and expertise to make this all… (more)

Subjects/Keywords: Vascular grafts; Hemodialysis; Stenosis; Hyperplasia

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APA (6^th Edition):

Vickers, D. B. (2008). Hyperthermia for treatment of hyperplasia in a hemodialysis access graft. (Masters Thesis). University of Utah. Retrieved from http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/1473/rec/598

Chicago Manual of Style (16^th Edition):

Vickers, Daniel Baker. “Hyperthermia for treatment of hyperplasia in a hemodialysis access graft.” 2008. Masters Thesis, University of Utah. Accessed March 06, 2021. http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/1473/rec/598.

MLA Handbook (7^th Edition):

Vickers, Daniel Baker. “Hyperthermia for treatment of hyperplasia in a hemodialysis access graft.” 2008. Web. 06 Mar 2021.

Vancouver:

Vickers DB. Hyperthermia for treatment of hyperplasia in a hemodialysis access graft. [Internet] [Masters thesis]. University of Utah; 2008. [cited 2021 Mar 06]. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/1473/rec/598.

Council of Science Editors:

Vickers DB. Hyperthermia for treatment of hyperplasia in a hemodialysis access graft. [Masters Thesis]. University of Utah; 2008. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/1473/rec/598

University of Limerick

2. Coakley, Daniel Nicholas Martin. Evaluation of xenogenic extracellular matrix scaffolds for use in tissue engineered vascular grafts.

Degree: 2015, University of Limerick

URL: http://hdl.handle.net/10344/4882

►

peer-reviewed

Existing synthetic vascular grafts have unacceptably high failure rates when replacing below knee arteries. In vitro endothelialisation is an emerging procedure that has been… (more)

▼

peer-reviewed

Existing synthetic vascular grafts have unacceptably high failure rates when replacing below knee arteries. In vitro endothelialisation is an emerging procedure that has been shown to enhance the patency rates of below knee synthetic vascular grafts. Here autologous endothelial cells are harvested and cultured on the lining of the graft prior to implantation. The technique is limited by the narrow time frame availalble for culture and the finite cells obtainable following harvest. As well as this, existing synthetic materials are poor cellular substrates and must be combined with coatings to promote cellular growth and attachment. The most common coated graft used clinically is fibrin-coated polytetrafluoroethylene (ePTFE). The study aim was to compare the endothelialisation of fibrin-coated ePTFE with emerging novel extracellular matrix (ECM) scaffolds, which we hypothesise will provide a superior substrate for cell growth by mimicking the in vivo environment more closely. We also construct and verify a novel subatmospheric bioreactor in order to enhance the proliferation of cultured cells. Finally, we examine the culturing of endothelial cells inside the lumen of small diameter ECM vascular grafts. Our Results showed that cells remained viable and produced von Willebrand factor on all substrates tested. There was no difference in adhesion rates between ECM scaffolds and fibrin-coated ePTFE (p = 1.00). Endothelial cells proliferated fastest on ECM scaffolds when compared to all other materials tested (p <0.001). When seeded scaffolds were exposed to subatmospheric pressures in a closed bioreactor a mechanical strain was exerted on the construct. This was shown to alter cellular morphology and enhance cellular proliferation. We found we could rapidly form a confluent endothelial lining on the luminal surface of small diameter ECM grafts. In conclusion, ECM bioscaffolds offer a superior substrate for promoting rapid endothelialisation compared to existing fibrin-coated ePTFE by combining firm cellular anchorage and rapid cell expansion. Proliferation rates were positively influenced by a subatmospheric bioreactor. This work suggests that ECM materials are promising scaffolds for use in small vessel tissue engineering.

Advisors/Committee Members: Robinson, J.S., Lavin, Michael.

Subjects/Keywords: synthetic vascular grafts; cardiovascular system

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APA (6^th Edition):

Coakley, D. N. M. (2015). Evaluation of xenogenic extracellular matrix scaffolds for use in tissue engineered vascular grafts. (Thesis). University of Limerick. Retrieved from http://hdl.handle.net/10344/4882

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Coakley, Daniel Nicholas Martin. “Evaluation of xenogenic extracellular matrix scaffolds for use in tissue engineered vascular grafts.” 2015. Thesis, University of Limerick. Accessed March 06, 2021. http://hdl.handle.net/10344/4882.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Coakley, Daniel Nicholas Martin. “Evaluation of xenogenic extracellular matrix scaffolds for use in tissue engineered vascular grafts.” 2015. Web. 06 Mar 2021.

Vancouver:

Coakley DNM. Evaluation of xenogenic extracellular matrix scaffolds for use in tissue engineered vascular grafts. [Internet] [Thesis]. University of Limerick; 2015. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/10344/4882.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Coakley DNM. Evaluation of xenogenic extracellular matrix scaffolds for use in tissue engineered vascular grafts. [Thesis]. University of Limerick; 2015. Available from: http://hdl.handle.net/10344/4882

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Gothenburg / Göteborgs Universitet

3. Simsa, Robin. Decellularization as a method to generate a new generation of vascular grafts.

Degree: 2020, University of Gothenburg / Göteborgs Universitet

URL: http://hdl.handle.net/2077/63616

► Decellularization of blood vessels is a technique to remove cells from the extracellular matrix (ECM), which can be used as a vascular graft for peripheral… (more)

Subjects/Keywords: tissue engineering; vascular grafts; cardiovascular disease; decellularization

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APA (6^th Edition):

Simsa, R. (2020). Decellularization as a method to generate a new generation of vascular grafts. (Thesis). University of Gothenburg / Göteborgs Universitet. Retrieved from http://hdl.handle.net/2077/63616

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Simsa, Robin. “Decellularization as a method to generate a new generation of vascular grafts.” 2020. Thesis, University of Gothenburg / Göteborgs Universitet. Accessed March 06, 2021. http://hdl.handle.net/2077/63616.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Simsa, Robin. “Decellularization as a method to generate a new generation of vascular grafts.” 2020. Web. 06 Mar 2021.

Vancouver:

Simsa R. Decellularization as a method to generate a new generation of vascular grafts. [Internet] [Thesis]. University of Gothenburg / Göteborgs Universitet; 2020. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/2077/63616.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Simsa R. Decellularization as a method to generate a new generation of vascular grafts. [Thesis]. University of Gothenburg / Göteborgs Universitet; 2020. Available from: http://hdl.handle.net/2077/63616

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Limerick

4. Davis, Laura M. Towards the development of a physiologically mimetic tissue engineered cardiovascular graft for the treatment of abdominal aortic aneurysms.

Degree: 2014, University of Limerick

URL: http://hdl.handle.net/10344/4208

►

peer-reviewed

With cardiovascular pathologies continuing to prevail, effective arterial replacements for the minimally invasive treatment of abdominal aortic aneurysms (AAAs) through endovascular aneurysm repair (EVAR)… (more)

▼

peer-reviewed

With cardiovascular pathologies continuing to prevail, effective arterial replacements for the minimally invasive treatment of abdominal aortic aneurysms (AAAs) through endovascular aneurysm repair (EVAR) are still an under-developed approach. A proposed solution to the identified problems of EVAR, including stent-graft migration and endoleaks, focuses on the use of a tissue engineered (TE) collar to create a biological seal between the device and the host artery. TE acellular vascular grafts are an emerging concept with the development and optimisation of biological scaffolds composed of naturally derived ECM, such as small intestinal submucosa (SIS) and urinary bladder matrix (UBM), receiving significant attention in the field of TE. In this research material characteristics such as scaffold surface topography and material wettability, of both naturally derived ECMs and comparative synthetically engineered materials were evaluated. As efficient endothelialisation is a desirable characteristic, cellular adhesion and proliferation as well as the interaction between the scaffold material and vascular cells were examined together with an assessment of scaffold mechanical behaviour. Overall, naturally derived ECM scaffolds demonstrated superior biocompatible surfaces for cellular growth, adhesion and proliferation while also exhibiting the biomechanical properties necessary to withstand the in vivo environment and providing improved compliance than currently employed synthetic materials. A factor currently limiting the success of biological scaffolds is effective shelf stable storage. This research demonstrated the viability, mechanical integrity and bioactive potential of ECM stent-grafts as suitable off-the-shelf vascular prosthetic devices. The effect of long-term hydrated storage in stented and un-stented configurations on the mechanical performance and bioactive potential of SIS and UBM were analysed. Additionally, inflammatory inducing cellular remnants leached after hydrated storage of single and multi-ply ECM scaffolds were quantified and the effect of such leached products on cellular proliferation was investigated. Residual DNA present in the scaffold after the hydration period was also evaluated. Results from these studies indicated a globally beneficial effect of hydrated storage on ECM material performance at both a biomechanical and biological level with hydration also facilitating the removal of residual cellular remnants from the ECM scaffolds prior to implantation. Due to the inherent similarities of ECM composition and the intended vascular site of implantation, the potential of decellularised porcine aortic tissue to develop a novel acellular TE collar material was investigated. Material decellularisation was examined with extensive evaluation of mechanical properties and residual cellular remnants. The resulting decellularised scaffolds demonstrated successful cellular adhesion indicating the potential of the material as a vascular graft upon…

Advisors/Committee Members: Walsh, Michael T., Carroll, Gráinne, McGloughlin, Timothy M., EI, European Regional Development Fund.

Subjects/Keywords: abdominal aortic aneurysms; ECMs; vascular grafts

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Davis, L. M. (2014). Towards the development of a physiologically mimetic tissue engineered cardiovascular graft for the treatment of abdominal aortic aneurysms. (Thesis). University of Limerick. Retrieved from http://hdl.handle.net/10344/4208

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Davis, Laura M. “Towards the development of a physiologically mimetic tissue engineered cardiovascular graft for the treatment of abdominal aortic aneurysms.” 2014. Thesis, University of Limerick. Accessed March 06, 2021. http://hdl.handle.net/10344/4208.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Davis, Laura M. “Towards the development of a physiologically mimetic tissue engineered cardiovascular graft for the treatment of abdominal aortic aneurysms.” 2014. Web. 06 Mar 2021.

Vancouver:

Davis LM. Towards the development of a physiologically mimetic tissue engineered cardiovascular graft for the treatment of abdominal aortic aneurysms. [Internet] [Thesis]. University of Limerick; 2014. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/10344/4208.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Davis LM. Towards the development of a physiologically mimetic tissue engineered cardiovascular graft for the treatment of abdominal aortic aneurysms. [Thesis]. University of Limerick; 2014. Available from: http://hdl.handle.net/10344/4208

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Waterloo

5. Pohan, Grace. Endothelialisation of Polyvinyl Alcohol Small Diameter Vascular Graft.

Degree: 2018, University of Waterloo

URL: http://hdl.handle.net/10012/13206

► As one of the possible treatments for cardiovascular disease (CVD), bypass grafting in small diameter vessel (≤ 6 mm diameter) is often employed. However, maintaining… (more)

▼ As one of the possible treatments for cardiovascular disease (CVD), bypass grafting in small diameter vessel (≤ 6 mm diameter) is often employed. However, maintaining the patency of this small diameter graft remains a challenge despite 50 years of research in this area. While ePTFE graft performance as a large diameter vascular graft is commendable and has been widely used in patients, the performance of ePTFE small diameter vascular graft is disappointing with 65% graft patency rate at 1 year.1 It was observed that graft occlusion is caused mainly by thrombus or intima hyperplasia (IH) formation. This study proposes the use of sodium trimetaphosphate (STMP) crosslinked polyvinyl alcohol (PVA) as small diameter vascular graft due to its low thrombogenicity and excellent mechanical property. However, PVA hydrophilicity is known to hinder any extracellular matrix (ECM) protein or cell attachment on its surface, while surface endothelialisation is believed to prevent thrombus and IH formation. We hypothesize that NH3 plasma luminal modification on the luminal surface of PVA along with hydrogel patterning with microtopography can improve surface interaction with endothelial cells. Additionally, we speculate the effect of terminal sterilization step on PVA grafts is minimum and the grafts can maintain its patency at 28 to 30-day timepoint. Endothelial cell attachment on PVA surface was found to improve on plasma treated luminal surface with 2 µm gratings topography. Endothelial gene expressions (PECAM1 and NOS3) were, however, found to be downregulated on plasma treated substrates. However, platelet and fibrin activation of the microgratings topography done with baboon ex vivo shunt model was comparable or lower than ePTFE graft control and collagen coated glass conduit. Furthermore, sterilization of PVA graft with γ-radiation was not found to have any toxic effect on cells and interestingly improve endothelialisation. While EtO sterilization was shown to alter PVA mechanical and material properties, γ-radiation only affected PVA graft mechanical compliance. Gamma irradiated small diameter PVA graft (2 mm ID) without any surface topography was implanted with end-to-side anastomoses at the right common carotid artery to assess PVA performance over 28-30 days in rabbit models. Five out of 7 PVA grafts were found patent after 28-30 days of implantation, while only 1 out of 2 ePTFE grafts were patent after 28-30 days. Endothelialisation of PVA graft in vivo was, however, not yet observed which compels the importance of plasma treatment and topography incorporation in the future work.

Subjects/Keywords: endothelial; vascular graft; polyvinyl alcohol; hydrogel; Endothelial seeding; Vascular grafts; Polyvinyl alcohol; Colloids

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APA (6^th Edition):

Pohan, G. (2018). Endothelialisation of Polyvinyl Alcohol Small Diameter Vascular Graft. (Thesis). University of Waterloo. Retrieved from http://hdl.handle.net/10012/13206

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Pohan, Grace. “Endothelialisation of Polyvinyl Alcohol Small Diameter Vascular Graft.” 2018. Thesis, University of Waterloo. Accessed March 06, 2021. http://hdl.handle.net/10012/13206.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Pohan, Grace. “Endothelialisation of Polyvinyl Alcohol Small Diameter Vascular Graft.” 2018. Web. 06 Mar 2021.

Vancouver:

Pohan G. Endothelialisation of Polyvinyl Alcohol Small Diameter Vascular Graft. [Internet] [Thesis]. University of Waterloo; 2018. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/10012/13206.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Pohan G. Endothelialisation of Polyvinyl Alcohol Small Diameter Vascular Graft. [Thesis]. University of Waterloo; 2018. Available from: http://hdl.handle.net/10012/13206

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of California – Berkeley

6. Huang, Fang. Engineering Biomaterials and Biomolecules for Vascular Regeneration.

Degree: Bioengineering, 2016, University of California – Berkeley

URL: http://www.escholarship.org/uc/item/47m0z167

► Tissue engineering and regenerative medicine is a multidisciplinary field that combines the knowledge and technologies of biology, materials, chemistry and medicine among others. Within the… (more)

▼ Tissue engineering and regenerative medicine is a multidisciplinary field that combines the knowledge and technologies of biology, materials, chemistry and medicine among others. Within the realm of tissue repair and regeneration, vascular reconstruction, in particular, is influenced by both biophysical and biochemical cues and it is critical to fully understand how these cues interact with the physiological microenvironment. In this dissertation, we demonstrated how host vascular regenerative potential can be harnessed by optimizing vascular grafts from both the biomaterial and biochemical perspectives. We utilized and optimized electrospinning technology to create microfibrous vascular grafts. We showed that incorporating a fast degrading polymer into the vascular grafts effectively enhances cell infiltration without majorly compromising its mechanical and structural properties. Our in vivo data suggests that although the optimized vascular graft was able to induce the initiation of vascular regeneration, the process was accelerated and enhanced with the help of biochemical cues presented in the form of stromal cell-derived factor-1α (SDF-1α). In the presence of chemokine SDF-1α, the vascular grafts demonstrated better wall remodeling and long-term patency. This observation again highlights the significance of both biomaterial and biochemical effects. While the vascular grafts in our studies might recruit inflammatory cells, calcification which is a destructive complication typically associated with inflammatory response was not observed. The final part of this dissertation details our efforts in using protein engineering to broaden the biological application of SDF-1α by introducing a free cysteine residue on its C-terminus. We showed that the cysteine-modified SDF-1α exhibits a bioactivity different from its unmodified counterpart, the underlying mechanism of which needs to be elucidated in future studies. Taken together, this work is expected to provide insights into optimization of vascular graft platforms for broad therapeutic applications.

Subjects/Keywords: Biomedical engineering; Biomaterials; Electrospinning; SDF-1α; Tissue Engineering; Vascular Grafts; Vascular Regeneration

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APA (6^th Edition):

Huang, F. (2016). Engineering Biomaterials and Biomolecules for Vascular Regeneration. (Thesis). University of California – Berkeley. Retrieved from http://www.escholarship.org/uc/item/47m0z167

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Huang, Fang. “Engineering Biomaterials and Biomolecules for Vascular Regeneration.” 2016. Thesis, University of California – Berkeley. Accessed March 06, 2021. http://www.escholarship.org/uc/item/47m0z167.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Huang, Fang. “Engineering Biomaterials and Biomolecules for Vascular Regeneration.” 2016. Web. 06 Mar 2021.

Vancouver:

Huang F. Engineering Biomaterials and Biomolecules for Vascular Regeneration. [Internet] [Thesis]. University of California – Berkeley; 2016. [cited 2021 Mar 06]. Available from: http://www.escholarship.org/uc/item/47m0z167.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Huang F. Engineering Biomaterials and Biomolecules for Vascular Regeneration. [Thesis]. University of California – Berkeley; 2016. Available from: http://www.escholarship.org/uc/item/47m0z167

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Virginia Commonwealth University

7. Wolfe, Patricia. Tissue Engineering an Acellular Bioresorbable Vascular Graft to Promote Regeneration.

Degree: PhD, Biomedical Engineering, 2011, Virginia Commonwealth University

URL: https://doi.org/10.25772/CSZQ-C202 ; https://scholarscompass.vcu.edu/etd/2627

► Tissue engineering is an interdisciplinary field that aims to restore, maintain, or improve diseased or damaged tissues. Electrospinning has become one of the most popular… (more)

▼ Tissue engineering is an interdisciplinary field that aims to restore, maintain, or improve diseased or damaged tissues. Electrospinning has become one of the most popular means to fabricate a scaffold for various tissue engineering applications as the process is extremely versatile and inexpensive. The ability for electrospinning to consistently create nanofibrous structures capable of mimicking the native extracellular matrix (ECM) is the basis behind why this technique is so successful in tissue engineering. Cardiovascular disease has been the leading cause of death in the United States for over 100 years, and because of this, the need for coronary artery replacements is in serious demand. More specifically, small diameter vessels (<6 mm I.D.) are most needed, due to the fact that they are most often affected and the current clinical replacements provide less than optimal long-term patency and regenerative ability. Tissue engineering of vascular grafts has been investigated for over 50 years, however, synthetic replacements made of Dacron® and expanded-poly(tetrafluoroethylene) (e-PTFE) still remain the clinical standard. This study examines a variety of different ways to alter different characteristics of electrospun constructs, to create scaffolds that would be favorable for use as a blood vessel replacement; the end goal being the creation of an acellular bioresorbable vascular graft that would provide sufficient mechanical support to withstand physiological forces, as well as ample biocompatibility to allow host cells to infiltrate and regenerate the graft as the structure degrades. As a way of tailoring the mechanical and thermal properties of a scaffold to be more conducive to that of a native artery, a novel co-polymer was created from the random copolymerization of two monomers; 1,4-Dioxan-2-one (DX) and DL-3-methyl-1,4-dioxan-2-one (DL-3-MeDX) were mixed at different ratios and electrospun, forming nanofibrous scaffolds that exhibited different mechanical and thermal properties. Next, scaffolds were electrospun from natural and synthetic polymers, and the potential for these materials to elicit the formation of an acute thrombotic occlusion was investigated by quantifying tissue factor expression from monocytes using a novel technique. Tissue factor expression by monocytes on the electrospun natural and synthetic polymer scaffolds was compared to that of e-PTFE to determine their potential for use as vascular graft materials. Platelet-rich plasma (PRP), a naturally occurring blood component which is comprised of supraphysiologic concentrations of autologous growth factors, was activated and lyophilized to form a preparation rich in growth factors (PRGF). PRGF was electrospun for the first time, to create a scaffold that would mimic the role of the native ECM in the wound healing cascade. Characterization of these scaffolds proved their bioactivity was enhanced, with cell infiltration occurring throughout the structures in as little as 3 days. Lastly, PRP/PRGF and/or heparin were incorporated into… Advisors/Committee Members: Gary Bowlin.

Subjects/Keywords: tissue engineering; electrospinning; vascular grafts; Biomedical Engineering and Bioengineering; Engineering

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wolfe, P. (2011). Tissue Engineering an Acellular Bioresorbable Vascular Graft to Promote Regeneration. (Doctoral Dissertation). Virginia Commonwealth University. Retrieved from https://doi.org/10.25772/CSZQ-C202 ; https://scholarscompass.vcu.edu/etd/2627

Chicago Manual of Style (16^th Edition):

Wolfe, Patricia. “Tissue Engineering an Acellular Bioresorbable Vascular Graft to Promote Regeneration.” 2011. Doctoral Dissertation, Virginia Commonwealth University. Accessed March 06, 2021. https://doi.org/10.25772/CSZQ-C202 ; https://scholarscompass.vcu.edu/etd/2627.

MLA Handbook (7^th Edition):

Wolfe, Patricia. “Tissue Engineering an Acellular Bioresorbable Vascular Graft to Promote Regeneration.” 2011. Web. 06 Mar 2021.

Vancouver:

Wolfe P. Tissue Engineering an Acellular Bioresorbable Vascular Graft to Promote Regeneration. [Internet] [Doctoral dissertation]. Virginia Commonwealth University; 2011. [cited 2021 Mar 06]. Available from: https://doi.org/10.25772/CSZQ-C202 ; https://scholarscompass.vcu.edu/etd/2627.

Council of Science Editors:

Wolfe P. Tissue Engineering an Acellular Bioresorbable Vascular Graft to Promote Regeneration. [Doctoral Dissertation]. Virginia Commonwealth University; 2011. Available from: https://doi.org/10.25772/CSZQ-C202 ; https://scholarscompass.vcu.edu/etd/2627

Queen Mary, University of London

8. Qui, Lin. Interaction between vascular endothelial cells and surface textured biomaterials.

Degree: PhD, 2014, Queen Mary, University of London

URL: http://qmro.qmul.ac.uk/xmlui/handle/123456789/8854 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.667301

► A promising approach to overcome thrombus and neointima formation on vascular grafts is to create a functional, quiescent monolayer of endothelial cells on the surface… (more)

▼ A promising approach to overcome thrombus and neointima formation on vascular grafts is to create a functional, quiescent monolayer of endothelial cells on the surface of implants. Surface topography of these implants is proven to enhance cell attachment and to reduce the inflammation associated with a smooth surface. Photoembossing is a relatively new, simple, environment-friendly and cost-effective technique to create surface topographies, since there is no etching step or mould needed. In this study, photopolymer films are photoembossed through contact mask photoembossing, while fibres are photoembossed through holographic lithography. Surface relief textures of ridges and grooves with various pitch sizes and heights are successfully obtained through both methods. Furthermore, we introduce this technique to fabricate, for the first time, reproducible surface textures on electrospun fibres. Human umbilical vein endothelial cells (HUVECs) are used in the study. Three different systems are investigated: non-degradable PMMA-TPETA, semi-degradable PLGA-TPETA and fully degradable PLGA-PEGDA-DTT, for different applications and therapeutic requirements. Both non-degradable PMMA-TPETA photopolymer and semi-degradable PLGA-TPETA photopolymer are shown to improve biocompatibility compared to PMMA and PLGA, respectively. Photoembossed films made from these two photopolymers show significantly improved cell attachment and proliferation, IV with a water contact angle around 70º. It is shown that the pitch size of surface topographies affects cell adhesion and migration in the wound healing assay study. Interaction between HUVECs and fibres shows that cells grow from their initial locations at fibre crossings. Focal adhesions are seen to be more aggregated on the surface textured fibres, while those on the glass cover slips are more dispersed near the edge of the cell membrane. The appearance of F-actin in the cytoplasm is also seen to be influenced by the surface topography, where changes in the diameter of the fibre and its surface texture result in F-actin rearrangement. Our study shows that a surface textured, fully degradable, gel-like photopolymer PLGA-PEGDA-DTT has great potential to be further developed for tissue engineering applications.

Subjects/Keywords: 610.28; Materials Science; Vascular grafts; Tissue Engineering; Biomaterials; Bioengineering

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APA (6^th Edition):

Qui, L. (2014). Interaction between vascular endothelial cells and surface textured biomaterials. (Doctoral Dissertation). Queen Mary, University of London. Retrieved from http://qmro.qmul.ac.uk/xmlui/handle/123456789/8854 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.667301

Chicago Manual of Style (16^th Edition):

Qui, Lin. “Interaction between vascular endothelial cells and surface textured biomaterials.” 2014. Doctoral Dissertation, Queen Mary, University of London. Accessed March 06, 2021. http://qmro.qmul.ac.uk/xmlui/handle/123456789/8854 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.667301.

MLA Handbook (7^th Edition):

Qui, Lin. “Interaction between vascular endothelial cells and surface textured biomaterials.” 2014. Web. 06 Mar 2021.

Vancouver:

Qui L. Interaction between vascular endothelial cells and surface textured biomaterials. [Internet] [Doctoral dissertation]. Queen Mary, University of London; 2014. [cited 2021 Mar 06]. Available from: http://qmro.qmul.ac.uk/xmlui/handle/123456789/8854 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.667301.

Council of Science Editors:

Qui L. Interaction between vascular endothelial cells and surface textured biomaterials. [Doctoral Dissertation]. Queen Mary, University of London; 2014. Available from: http://qmro.qmul.ac.uk/xmlui/handle/123456789/8854 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.667301

Cal Poly

9. Berry, Carolyn. DESIGN AND DEVELOPMENT OF TWO TEST FIXTURES TO TEST THE LONGITUDINAL AND TRANSVERSE TENSILE PROPERTIES OF SMALL DIAMETER TUBULAR POLYMERS.

Degree: MS, Biomedical and General Engineering, 2011, Cal Poly

URL: https://digitalcommons.calpoly.edu/theses/494 ; 10.15368/theses.2011.49

► Hundreds of thousands of vascular bypass grafts are implanted in the United States every year, but there has yet to be an ideal graft… (more)

▼ Hundreds of thousands of vascular bypass grafts are implanted in the United States every year, but there has yet to be an ideal graft material to substitute for one’s own autologous vessel. Many synthetic materials have been shown to be successful vessel replacements; however, none have been proven to exhibit the same mechanical properties as native vessels, one of the most important criteria in selecting a vascular graft material. Part of this issue is due to the fact that, currently, there is no “gold standard” for testing the longitudinal and transverse tensile properties of small diameter tubular materials. While there are ASTM and ISO standards that suggest ways to test tubes in their original form, many researchers have published tensile strength data based on cutting the tube and testing it as a flat sample. Thus, it was the aim of this thesis to understand, establish, and implement accurate tensile testing methods of small diameter polymers in their original, tubular state on Cal Poly’s campus. Two test fixtures were created based on specified design criteria in order to test materials in their tubular form in both the longitudinal and transverse directions. Both fixtures were successful in testing PLGA and ePTFE samples, and statistical data was gathered for the transverse test fixture. The new transverse test fixture was tested against the current method of testing, and a significant (α = 0.05) difference between methods was established for ultimate tensile strength. This analysis, however, cannot determine which test method is more accurate, thus more extensive testing is required to verify the design of both fixtures. By developing a method for testing small diameter polymers in tubular form on Cal Poly’s campus, it allows for more testing of various small diameter tubes and more comparative data to validate each design. It also demonstrates a need for a more detailed and widespread standardization of testing for small diameter tubes, especially in vascular substitute applications where the ideal vessel replacement has yet to be found. Advisors/Committee Members: Kristen O'Halloran Cardinal.

Subjects/Keywords: tensile properties; tubular polymers; vascular grafts; tensile testing; Biomaterials

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APA (6^th Edition):

Berry, C. (2011). DESIGN AND DEVELOPMENT OF TWO TEST FIXTURES TO TEST THE LONGITUDINAL AND TRANSVERSE TENSILE PROPERTIES OF SMALL DIAMETER TUBULAR POLYMERS. (Masters Thesis). Cal Poly. Retrieved from https://digitalcommons.calpoly.edu/theses/494 ; 10.15368/theses.2011.49

Chicago Manual of Style (16^th Edition):

Berry, Carolyn. “DESIGN AND DEVELOPMENT OF TWO TEST FIXTURES TO TEST THE LONGITUDINAL AND TRANSVERSE TENSILE PROPERTIES OF SMALL DIAMETER TUBULAR POLYMERS.” 2011. Masters Thesis, Cal Poly. Accessed March 06, 2021. https://digitalcommons.calpoly.edu/theses/494 ; 10.15368/theses.2011.49.

MLA Handbook (7^th Edition):

Berry, Carolyn. “DESIGN AND DEVELOPMENT OF TWO TEST FIXTURES TO TEST THE LONGITUDINAL AND TRANSVERSE TENSILE PROPERTIES OF SMALL DIAMETER TUBULAR POLYMERS.” 2011. Web. 06 Mar 2021.

Vancouver:

Berry C. DESIGN AND DEVELOPMENT OF TWO TEST FIXTURES TO TEST THE LONGITUDINAL AND TRANSVERSE TENSILE PROPERTIES OF SMALL DIAMETER TUBULAR POLYMERS. [Internet] [Masters thesis]. Cal Poly; 2011. [cited 2021 Mar 06]. Available from: https://digitalcommons.calpoly.edu/theses/494 ; 10.15368/theses.2011.49.

Council of Science Editors:

Berry C. DESIGN AND DEVELOPMENT OF TWO TEST FIXTURES TO TEST THE LONGITUDINAL AND TRANSVERSE TENSILE PROPERTIES OF SMALL DIAMETER TUBULAR POLYMERS. [Masters Thesis]. Cal Poly; 2011. Available from: https://digitalcommons.calpoly.edu/theses/494 ; 10.15368/theses.2011.49

Georgia Tech

10. Greer, Linda S. Material property testing of a collagen/smooth muscle cell gel for the development of a tissue engineered vascular graft.

Degree: MS, Mechanical engineering, 1994, Georgia Tech

URL: http://hdl.handle.net/1853/33447

Subjects/Keywords: Tissues; Biomedical engineering; Vascular grafts

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APA (6^th Edition):

Greer, L. S. (1994). Material property testing of a collagen/smooth muscle cell gel for the development of a tissue engineered vascular graft. (Masters Thesis). Georgia Tech. Retrieved from http://hdl.handle.net/1853/33447

Chicago Manual of Style (16^th Edition):

Greer, Linda S. “Material property testing of a collagen/smooth muscle cell gel for the development of a tissue engineered vascular graft.” 1994. Masters Thesis, Georgia Tech. Accessed March 06, 2021. http://hdl.handle.net/1853/33447.

MLA Handbook (7^th Edition):

Greer, Linda S. “Material property testing of a collagen/smooth muscle cell gel for the development of a tissue engineered vascular graft.” 1994. Web. 06 Mar 2021.

Vancouver:

Greer LS. Material property testing of a collagen/smooth muscle cell gel for the development of a tissue engineered vascular graft. [Internet] [Masters thesis]. Georgia Tech; 1994. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/1853/33447.

Council of Science Editors:

Greer LS. Material property testing of a collagen/smooth muscle cell gel for the development of a tissue engineered vascular graft. [Masters Thesis]. Georgia Tech; 1994. Available from: http://hdl.handle.net/1853/33447

Virginia Tech

11. Gurjarpadhye, Abhijit Achyut. Dynamic Non-Destructive Monitoring of Bioengineered Blood Vessel Development within a Bioreactor using Multi-Modality Imaging.

Degree: PhD, Biomedical Engineering, 2013, Virginia Tech

URL: http://hdl.handle.net/10919/51359

► Regenerative medicine involves formation of tissue or organ for replacement of a wounded or dysfunctional tissue. Healthy cells extracted from the patient are expanded and… (more)

▼ Regenerative medicine involves formation of tissue or organ for replacement of a wounded or dysfunctional tissue. Healthy cells extracted from the patient are expanded and are seeded on a three-dimensional biodegradable scaffold. The structure is then placed in a bioreactor and is provided with nutrients for the cells, which proliferate and migrate throughout the scaffold to eventually form a desired to tissue that can be transplanted into the patient\'s body. Inability to monitor this complex process of regeneration in real-time makes control and optimization of this process extremely difficult. Histology, the gold standard used for tissue structural assessment, is a static technique that only provides "snapshots" of the progress and requires the specimen to be sacrificed. This inefficiency severely limits our understanding of the biological processes associated with tissue growth during the in vitro pre-conditioning phase. Optical Coherence Tomography (OCT) enables imaging of cross sectional structure in biological tissues by measuring the echo time delay of backreflected light. OCT has recently emerged as an important method to assess the structures of physiological, pathological as well as tissue engineered blood vessels. The goal of the present study is to develop an imaging system for non-destructive monitoring of blood vessels maturing within a bioreactor. Non-destructive structural imaging of tissue-engineered blood vessels cultured in a novel bioreactor was performed using free-space and catheter-based OCT imaging, while monitoring of the endothelium development was performed using a fluorescence imaging system that utilizes a commercial OCT catheter. The project included execution of three specific aims. Firstly, we developed OCT instrumentation to determine geometrical and optical properties of porcine and human skin in real-time. The purpose of the second aim was to assess structural development of tissue-engineered blood vessels maturing in a bioreactor. We constructed a novel quartz-based bioreactor that will permit free space and catheter-based OCT imaging of vascular grafts. The grafts were made of biodegradable PCL-collagen and seeded with multipotent mesenchymal cells. We imaged the maturing grafts over 30 days to assess changes in graft wall thickness. We also monitored change in optical properties of the grafts based on free-space OCT scanning. Finally, in order to visualize the proliferation of endothelial cells and development of the endothelium, we developed an imaging system that utilizes a commercial OCT catheter for single-cell-level imaging of the growing endothelium of a tissue-engineered blood vessel. We have developed two modules of an imaging system for non-destructive monitoring of maturing bioengineered vascular grafts. The first module provides the ability to non-destructively examine the structure of the grafts while the second module can track the progress of endothelialization. As both modules use the same endoscope for imaging, when operated in… Advisors/Committee Members: Rylander, Christopher G. (committeechair), Rylander, M Nichole (committee member), Wang, Ge (committee member), Soker, Shay (committee member), Xu, Yong (committee member).

Subjects/Keywords: Optical Coherence Tomography; Fluorescence Imaging; Vascular Grafts; Bioreactor; Non-destructive

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APA (6^th Edition):

Gurjarpadhye, A. A. (2013). Dynamic Non-Destructive Monitoring of Bioengineered Blood Vessel Development within a Bioreactor using Multi-Modality Imaging. (Doctoral Dissertation). Virginia Tech. Retrieved from http://hdl.handle.net/10919/51359

Chicago Manual of Style (16^th Edition):

Gurjarpadhye, Abhijit Achyut. “Dynamic Non-Destructive Monitoring of Bioengineered Blood Vessel Development within a Bioreactor using Multi-Modality Imaging.” 2013. Doctoral Dissertation, Virginia Tech. Accessed March 06, 2021. http://hdl.handle.net/10919/51359.

MLA Handbook (7^th Edition):

Gurjarpadhye, Abhijit Achyut. “Dynamic Non-Destructive Monitoring of Bioengineered Blood Vessel Development within a Bioreactor using Multi-Modality Imaging.” 2013. Web. 06 Mar 2021.

Vancouver:

Gurjarpadhye AA. Dynamic Non-Destructive Monitoring of Bioengineered Blood Vessel Development within a Bioreactor using Multi-Modality Imaging. [Internet] [Doctoral dissertation]. Virginia Tech; 2013. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/10919/51359.

Council of Science Editors:

Gurjarpadhye AA. Dynamic Non-Destructive Monitoring of Bioengineered Blood Vessel Development within a Bioreactor using Multi-Modality Imaging. [Doctoral Dissertation]. Virginia Tech; 2013. Available from: http://hdl.handle.net/10919/51359

University of Oklahoma

12. Goktas, Selda. Development of a Novel Ex Vivo Scaffold for the Regeneration of Periodontal Tissues.

Degree: PhD, 2011, University of Oklahoma

URL: http://hdl.handle.net/11244/318693

► In order to create functional HUV bioscaffolds for periodontal tissue engineering applications, in vitro 3D culture conditions were developed and optimized. The goal was to… (more)

Subjects/Keywords: Guided tissue regeneration; Vascular grafts; Tissue engineering; Periodontal disease – Treatment

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APA (6^th Edition):

Goktas, S. (2011). Development of a Novel Ex Vivo Scaffold for the Regeneration of Periodontal Tissues. (Doctoral Dissertation). University of Oklahoma. Retrieved from http://hdl.handle.net/11244/318693

Chicago Manual of Style (16^th Edition):

Goktas, Selda. “Development of a Novel Ex Vivo Scaffold for the Regeneration of Periodontal Tissues.” 2011. Doctoral Dissertation, University of Oklahoma. Accessed March 06, 2021. http://hdl.handle.net/11244/318693.

MLA Handbook (7^th Edition):

Goktas, Selda. “Development of a Novel Ex Vivo Scaffold for the Regeneration of Periodontal Tissues.” 2011. Web. 06 Mar 2021.

Vancouver:

Goktas S. Development of a Novel Ex Vivo Scaffold for the Regeneration of Periodontal Tissues. [Internet] [Doctoral dissertation]. University of Oklahoma; 2011. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/11244/318693.

Council of Science Editors:

Goktas S. Development of a Novel Ex Vivo Scaffold for the Regeneration of Periodontal Tissues. [Doctoral Dissertation]. University of Oklahoma; 2011. Available from: http://hdl.handle.net/11244/318693

Drexel University

13. Ostroha, Jamie Lyn. Peptide-modified polymer for endothelialization.

Degree: 2001, Drexel University

URL: http://hdl.handle.net/1860/73

►

A functionalized polycarbonate material derived from tyrosine containing varyinglevels of free acid functionality along the backbone, poly(DTE-co-X%DT carbonate), hasbeen functionalized with a peptide to promote… (more)

▼

A functionalized polycarbonate material derived from tyrosine containing varyinglevels of free acid functionality along the backbone, poly(DTE-co-X%DT carbonate), hasbeen functionalized with a peptide to promote endothelial cell attachment and evaluatedas a potential synthetic vascular graft material. Lysine derivatives have been produced topromote the attachment of engineered peptides for promoting endothelialization.NMR has been used to analyze the initial structure of the poly(DTEC-co-X%DTC) and changes in the structure with the attachment of L-lysine ethyl ester andpolylysine, polyalanine, and the (ARKKAAKA)4 peptide. The polymer peak at 4.8 ppmhas been labeled the a-peak. This peak is the primary peak used to evaluate changes inthe spectra related to changing polymer functionality and the attachment of molecules tothe pendant acid group. Changes in the Ã-peak (3.05 ppm), the R2 peak (4.1 ppm) and theR3 peak (1.3 ppm) are compared to the a-peak for quantification purposes. Key NMRpeaks have been identified and lysine attachment and quantification is discussed. A lysinepeak has been identified at 4.38 ppm. This peak is also compared to the polymer a-peak,indicating 85-90% attachment efficiency. The use of polylysine, polyalanine, andpeptides establish that peptide binding is successful; qualitative and semi-quantitativeresults are presented. Semi-quantitative results with polyalanine indicate a large degree ofattachment, although some interference with the spectral peaks has been noted.The effect of poly(DTEC-co-X%DTC) on human endothelial cell attachment andgrowth is also studied and compared to current synthetic materials. Cell attachment hasbeen shown to increase significantly as the polymer becomes more hydrophilic. Nonfunctionalizedpolymers show that less than five percent of cells remained attach at aforce of 300 g, while 50% functionalized polymers show 40% attachment at the sameforce. Cell growth studies show an increase in cell affinity with increasing hydophilicity.35% functionalized polymer shows over double the number of cells as the nonfunctionalizedmaterial after seven days. The results for the p(DTEC) at increasing levelsof functionality bracket Dacron and PLA depending. Cell spreading, confluence, anddivision have been confirmed by visual analysis.

M.S., Materials Engineering – Drexel University, 2001

Advisors/Committee Members: Twardowski, Thomas.

Subjects/Keywords: Vascular grafts; Polymers in medicine

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APA (6^th Edition):

Ostroha, J. L. (2001). Peptide-modified polymer for endothelialization. (Thesis). Drexel University. Retrieved from http://hdl.handle.net/1860/73

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ostroha, Jamie Lyn. “Peptide-modified polymer for endothelialization.” 2001. Thesis, Drexel University. Accessed March 06, 2021. http://hdl.handle.net/1860/73.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ostroha, Jamie Lyn. “Peptide-modified polymer for endothelialization.” 2001. Web. 06 Mar 2021.

Vancouver:

Ostroha JL. Peptide-modified polymer for endothelialization. [Internet] [Thesis]. Drexel University; 2001. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/1860/73.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ostroha JL. Peptide-modified polymer for endothelialization. [Thesis]. Drexel University; 2001. Available from: http://hdl.handle.net/1860/73

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

14. Burrows, Mariana Carvalho. Suportes híbridos de PET e colágeno como modelo para enxertia vascular.

Degree: Mestrado, Química Orgânica, 2011, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/46/46135/tde-25042011-133342/ ;

►

Suportes eletrofiados para crescimento celular são de interesse para a engenharia de tecidos, principalmente em função de sua estrutura em forma de rede tridimensional de… (more)

▼

Suportes eletrofiados para crescimento celular são de interesse para a engenharia de tecidos, principalmente em função de sua estrutura em forma de rede tridimensional de fibras de diâmetro nanométrico. Esta arquitetura especial permite a geração de elevada área superficial e porosidade, características importantes para a adesão, proliferação e infiltração de células para o interior do suporte. A utilização de um suporte eletrofiado como enxerto vascular necessita ainda que este apresente excelentes propriedades mecânicas, associadas a uma elevada biocompatibilidade. Neste trabalho mostramos que estas propriedades podem ser alcançadas a partir da eletrofiação de uma co-solução de PET e colágeno gerando um material híbrido, visto que PET apresenta excelentes propriedades mecânicas e o colágeno é o principal componente da matriz extracelular. A obtenção dos suportes eletrofiados de PETcolágeno mostrou ser possível utilizando-se como solvente HFIP e HFIP/TFA 7:2. No entanto, neste último, o colágeno é completamente degradado durante o processo de solubilização. Fixando-se os parâmetros de eletrofiação, a morfologia da malha obtida mostrou ser dependente da relação massa PET/massa colágeno, concentração total da solução e solvente utilizado. Foram obtidos materiais com distribuição de diâmetros unimodal e bimodal, além de materiais com formato em fitas e teias entre as fibras. Ainda, PET e colágeno formam malhas de composição complexa, nas quais são encontradas fibras compostas de materiais puros, mas também formam blendas em que os dois materiais encontram-se misturados em uma mesma fibra. Os materiais S8,2 S4,6 foram caracterizados química-, mecânica- e biologicamente. Observou-se que, para filmes planos, estes materiais apresentaram energia de superfície mais próxima da do colágeno, o que justifica a melhor adesão celular em S8,2 e S4,6 do que no PET. S8,2 mostrou ter valores de módulo de elasticidade e elongação máxima próximos ao da artéria femoral, enquanto que S4,6 apresentou-se como um material quebradiço. Os ensaios de crescimento celular utilizando fibroblastos, um modelo de tecido conjuntivo (linhagem 3T3-L1) e células endoteliais, um modelo de tecido arterial e venoso (HUVECs) comprovaram a excelente adesão e proliferação celular nos suportes celulares. S8,2 apresentou-se como o melhor material frente às células HUVECS, enquanto que S4,6 foi o melhor material frente às células 3T3-L1. Propõe-se a utilização de S8,2 como um biomaterial para enxertia vascular e S4,6 como material de recobrimento de próteses já utilizadas.

Scaffolds obtained by electrospinning for cellular growth are of interest for materials engineering, especially considering its structure in the form of a three-dimensional fiber mesh of nanometric diameter. This special architecture allows the generation of larger surface areas and higher porosity structures, and also important characteristics for the adhesion, proliferation and infiltration of cells into the scaffold. The use of an electrospun scaffold as a vascular graft additionally…

Advisors/Committee Members: Catalani, Luiz Henrique.

Subjects/Keywords: Colágeno; Collagen; Electrospinning; Electrospun scaffolds; Eletrofiação; Enxertos vasculares; PET; PET; Suportes eletrofiados; Vascular grafts

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APA (6^th Edition):

Burrows, M. C. (2011). Suportes híbridos de PET e colágeno como modelo para enxertia vascular. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/46/46135/tde-25042011-133342/ ;

Chicago Manual of Style (16^th Edition):

Burrows, Mariana Carvalho. “Suportes híbridos de PET e colágeno como modelo para enxertia vascular.” 2011. Masters Thesis, University of São Paulo. Accessed March 06, 2021. http://www.teses.usp.br/teses/disponiveis/46/46135/tde-25042011-133342/ ;.

MLA Handbook (7^th Edition):

Burrows, Mariana Carvalho. “Suportes híbridos de PET e colágeno como modelo para enxertia vascular.” 2011. Web. 06 Mar 2021.

Vancouver:

Burrows MC. Suportes híbridos de PET e colágeno como modelo para enxertia vascular. [Internet] [Masters thesis]. University of São Paulo; 2011. [cited 2021 Mar 06]. Available from: http://www.teses.usp.br/teses/disponiveis/46/46135/tde-25042011-133342/ ;.

Council of Science Editors:

Burrows MC. Suportes híbridos de PET e colágeno como modelo para enxertia vascular. [Masters Thesis]. University of São Paulo; 2011. Available from: http://www.teses.usp.br/teses/disponiveis/46/46135/tde-25042011-133342/ ;

Stellenbosch University

15. Janson, Jacques. Evaluating a bioprosthetic anterior mitral valve leaflet made from autologous jugular vein and expanded polytetrafluoroethylene (Gore-Tex) chordae in a sheep model.

Degree: PhD, Medicine, 2016, Stellenbosch University

URL: http://hdl.handle.net/10019.1/98398

►

ENGLISH ABSTRACT: The purpose of this study was to evaluate whether an autologous vein graft supported by expanded polytetrafluoroethylene (Gore-Tex) chordae can be used to… (more)

▼

ENGLISH ABSTRACT: The purpose of this study was to evaluate whether an autologous vein graft supported by expanded polytetrafluoroethylene (Gore-Tex) chordae can be used to replace an anterior mitral valve leaflet and whether the vein will be able to withstand the stress and strain of deformation, remain viable in the intracardiac environment and be able to adapt morphologically and grow as a valve leaflet. An autologous jugular vein graft, used as a double layer, supported by Gore-Tex chordae was used to create a functional anterior mitral valve leaflet in 21 sheep. No ring annuloplasty was used to support the annulus. The average cross-clamp time was 99 minutes (76 to 151 min) and the average bypass time was 137 minutes (109 to 188 min). One sheep died intra-operatively. The post-operative echocardiogram demonstrated laminar diastolic flow across the mitral valve with an average opening area of 2.8cm². Fourteen sheep had trace to mild mitral regurgitation (MR), 5 sheep had mild to moderate MR and 1 sheep had moderate to severe MR. The body of the vein leaflet tends to billow during systole which increases stress on the Gore-Tex chordae. Three sheep died 2 to 3 days postoperatively from mitral regurgitation due to Gore-Tex chordae that were too long, causing prolapse in 2 cases and 1 case developed a hematoma between the 2 vein layers. Seven sheep died between 1 and 6 months. Four sheep developed infective endocarditis on the mitral valve. Echocardiography at 6 months showed that the mitral regurgitation (MR) progressed with time in most of the sheep: 3 out of 11 sheep had mild MR, 5 had mild to moderate MR and 3 had moderate to severe MR. The progression of MR was due to lack of secondary chordal support of the vein leaflet and mitral annulus, leading to progressive annular dilatation, decreased leaflet coaptation length and increased tension on the primary Gore-Tex chordae. Durability of the valve should be improved by adding an annuloplasty ring and supporting the leaflet with secondary chordae. The 10 surviving sheep were euthanized between 6 to 10 months. All vein implants were examined histologically. The vein leaflet developed intimal fibroplasia and fibrous proliferation between the 2 vein layers as a response to the increased stress upon the tissue. This caused leaflet thickening, but the vein remained flexible without shortening or contracture. The 6 to 10 month vein implants showed viable endothelium and the underlying vein layers clearly showed viability with myofibroblasts, collagen and elastin. A normal healing pattern was seen at the suture lines and no calcification was seen in the vein leaflet apart from the Gore-Tex sutures. No vein growth was demonstrated. Autologous vein has the potential to be used as a valve leaflet substitute, because it remains viable in the intracardiac position for up to 10 months and is able to withstand the stress and deformation of a valve leaflet. Histologically it showed the ability to heal and to morphologically adapt to the new environment.

AFRIKAANSE…

Advisors/Committee Members: Coetzee, Andre, Stellenbosch University. Faculty of Medicine and Health Sciences. Dept. of Medicine. Cardiology..

Subjects/Keywords: Vascular grafts; Veins – Transplantation; Mitral valve – Surgery; Heart valve prosthesis; UCTD; Animal models in research

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APA (6^th Edition):

Janson, J. (2016). Evaluating a bioprosthetic anterior mitral valve leaflet made from autologous jugular vein and expanded polytetrafluoroethylene (Gore-Tex) chordae in a sheep model. (Doctoral Dissertation). Stellenbosch University. Retrieved from http://hdl.handle.net/10019.1/98398

Chicago Manual of Style (16^th Edition):

Janson, Jacques. “Evaluating a bioprosthetic anterior mitral valve leaflet made from autologous jugular vein and expanded polytetrafluoroethylene (Gore-Tex) chordae in a sheep model.” 2016. Doctoral Dissertation, Stellenbosch University. Accessed March 06, 2021. http://hdl.handle.net/10019.1/98398.

MLA Handbook (7^th Edition):

Janson, Jacques. “Evaluating a bioprosthetic anterior mitral valve leaflet made from autologous jugular vein and expanded polytetrafluoroethylene (Gore-Tex) chordae in a sheep model.” 2016. Web. 06 Mar 2021.

Vancouver:

Janson J. Evaluating a bioprosthetic anterior mitral valve leaflet made from autologous jugular vein and expanded polytetrafluoroethylene (Gore-Tex) chordae in a sheep model. [Internet] [Doctoral dissertation]. Stellenbosch University; 2016. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/10019.1/98398.

Council of Science Editors:

Janson J. Evaluating a bioprosthetic anterior mitral valve leaflet made from autologous jugular vein and expanded polytetrafluoroethylene (Gore-Tex) chordae in a sheep model. [Doctoral Dissertation]. Stellenbosch University; 2016. Available from: http://hdl.handle.net/10019.1/98398

Texas A&M University

16. Dempsey, David Kandell. Development and Characterization of Novel Biomaterials for Fabrication of Multilayered Vascular Grafts.

Degree: PhD, Biomedical Engineering, 2013, Texas A&M University

URL: http://hdl.handle.net/1969.1/151874

► Current synthetic alternatives to autologous grafts have often failed in small diameter applications (<6 mm) due to their thrombogenicity and compliance mismatch of native vasculature.… (more)

▼ Current synthetic alternatives to autologous grafts have often failed in small diameter applications (<6 mm) due to their thrombogenicity and compliance mismatch of native vasculature. No known synthetic material is capable of providing a non-thrombogenic inner layer that promotes endothelial cell (EC) interactions while also providing sufficient compliance and burst pressure for long term success in vivo. We have developed a multilayer design with an inner thromboresistant poly(ethylene glycol) (PEG) hydrogel based on Scl2-2 proteins designed to promote rapid in situ endothelialization. The bioactive component is reinforced with an electrospun segmented polyurethane (SPU) layer with tunable mechanical properties to withstand physiological loading conditions. The modulating of electrospun parameters to influence graft architecture coupled with the tunability of SPU mechanical performance is expected to give rise to improved graft biomechanical properties. Unfortunately, this advantage was found to be limited to only one property at a time. To this end, we have developed a novel semi-IPN approach to expand the range of possible graft compliance and burst pressures in order to simultaneously achieve biomechanical properties that exceed autologous veins. In addition to matching biomechanical properties, probability of long term success is also dependent on the grafts retention of perfomance despite cell-mediated attack. Typically, in the case poly(ether urethanes) (PEUs) and poly(carbonate urethanes) (PCUs), oxidative stability is the primary focus in the development of biostable SPUs. We have characterized the biostability of several commercially available polyurethanes while simultaneously evaluating the predictive capabilities of two main in vitro test methods to optimize our graft’s design. Despite ensured long term performance through optimizing biostability, a permanent scaffold prevents vasoactivity, a key function of vasculature. We have taken a tissue engineering approach to restorate vasoactivity by evaluating a novel aromatic biodegradable poly(ester urethane) (PEsU) for the reinforcing layer of a tissue engineering vascular graft (TEVG). This PEsU was expected to degrade into safe byproducts given its design based on glycolic acid and ethylene glycol. Following characterization, the PEsU was determined to be a strong potential reinforcing layer of a potential TEVG design. In summary, we have improved small diameter grafts through a multilayer design approach. Our graft demonstrated favorable initial fabrication feasibility, promising in vivo testing, biomechanical properties exceeding autologous veins, and strong oxidative stability. Overall, we have optimized the reinforcing layer through improvement of biomechanical properties via modulated material chemistry, optimized biostability, and identification of a biodegradable component expected to allow for restored vasoactivity. Advisors/Committee Members: Cosgriff-Hernandez, Elizabeth M (advisor), Grunlan, Melissa (committee member), Maitland, Duncan (committee member), Hahn, Mariah (committee member).

Subjects/Keywords: Coronary Artery Disease; small diameter vascular grafts; polyurethanes; semi-interpenetrating networks; oxidation; hydrolysis; electrospinning

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APA (6^th Edition):

Dempsey, D. K. (2013). Development and Characterization of Novel Biomaterials for Fabrication of Multilayered Vascular Grafts. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/151874

Chicago Manual of Style (16^th Edition):

Dempsey, David Kandell. “Development and Characterization of Novel Biomaterials for Fabrication of Multilayered Vascular Grafts.” 2013. Doctoral Dissertation, Texas A&M University. Accessed March 06, 2021. http://hdl.handle.net/1969.1/151874.

MLA Handbook (7^th Edition):

Dempsey, David Kandell. “Development and Characterization of Novel Biomaterials for Fabrication of Multilayered Vascular Grafts.” 2013. Web. 06 Mar 2021.

Vancouver:

Dempsey DK. Development and Characterization of Novel Biomaterials for Fabrication of Multilayered Vascular Grafts. [Internet] [Doctoral dissertation]. Texas A&M University; 2013. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/1969.1/151874.

Council of Science Editors:

Dempsey DK. Development and Characterization of Novel Biomaterials for Fabrication of Multilayered Vascular Grafts. [Doctoral Dissertation]. Texas A&M University; 2013. Available from: http://hdl.handle.net/1969.1/151874

Texas Tech University

17. Zuniga, Patricia O. Enhanced selectivity of a multifunctional biocatalyst, plasmin.

Degree: MS, Chemical Engineering, 1985, Texas Tech University

URL: http://hdl.handle.net/2346/59390

Subjects/Keywords: Plasmin; Fibrinolytic agents; Vascular grafts; Collagen

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APA (6^th Edition):

Zuniga, P. O. (1985). Enhanced selectivity of a multifunctional biocatalyst, plasmin. (Masters Thesis). Texas Tech University. Retrieved from http://hdl.handle.net/2346/59390

Chicago Manual of Style (16^th Edition):

Zuniga, Patricia O. “Enhanced selectivity of a multifunctional biocatalyst, plasmin.” 1985. Masters Thesis, Texas Tech University. Accessed March 06, 2021. http://hdl.handle.net/2346/59390.

MLA Handbook (7^th Edition):

Zuniga, Patricia O. “Enhanced selectivity of a multifunctional biocatalyst, plasmin.” 1985. Web. 06 Mar 2021.

Vancouver:

Zuniga PO. Enhanced selectivity of a multifunctional biocatalyst, plasmin. [Internet] [Masters thesis]. Texas Tech University; 1985. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/2346/59390.

Council of Science Editors:

Zuniga PO. Enhanced selectivity of a multifunctional biocatalyst, plasmin. [Masters Thesis]. Texas Tech University; 1985. Available from: http://hdl.handle.net/2346/59390

Texas Tech University

18. Ho, Chih-huang. The effects of design and surgical parameters on the anti-platelet and fibrinolytic activities of heparin/plasmin bound collageno-elastic tubes.

Degree: Chemistry, 1988, Texas Tech University

URL: http://hdl.handle.net/2346/21505

Subjects/Keywords: Prosthesis; Blood vessel prosthesis; Vascular grafts

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APA (6^th Edition):

Ho, C. (1988). The effects of design and surgical parameters on the anti-platelet and fibrinolytic activities of heparin/plasmin bound collageno-elastic tubes. (Thesis). Texas Tech University. Retrieved from http://hdl.handle.net/2346/21505

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ho, Chih-huang. “The effects of design and surgical parameters on the anti-platelet and fibrinolytic activities of heparin/plasmin bound collageno-elastic tubes.” 1988. Thesis, Texas Tech University. Accessed March 06, 2021. http://hdl.handle.net/2346/21505.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ho, Chih-huang. “The effects of design and surgical parameters on the anti-platelet and fibrinolytic activities of heparin/plasmin bound collageno-elastic tubes.” 1988. Web. 06 Mar 2021.

Vancouver:

Ho C. The effects of design and surgical parameters on the anti-platelet and fibrinolytic activities of heparin/plasmin bound collageno-elastic tubes. [Internet] [Thesis]. Texas Tech University; 1988. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/2346/21505.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ho C. The effects of design and surgical parameters on the anti-platelet and fibrinolytic activities of heparin/plasmin bound collageno-elastic tubes. [Thesis]. Texas Tech University; 1988. Available from: http://hdl.handle.net/2346/21505

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Missouri – Columbia

19. Ostdiek, Allison M. Development of a nanomaterial-tissue patch for vascular and cardiac reconstruction.

Degree: 2014, University of Missouri – Columbia

URL: http://hdl.handle.net/10355/45772

Subjects/Keywords: Biomedical materials; Vascular grafts; Vascular endothelial cells; Nanostructured materials

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APA (6^th Edition):

Ostdiek, A. M. (2014). Development of a nanomaterial-tissue patch for vascular and cardiac reconstruction. (Thesis). University of Missouri – Columbia. Retrieved from http://hdl.handle.net/10355/45772

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ostdiek, Allison M. “Development of a nanomaterial-tissue patch for vascular and cardiac reconstruction.” 2014. Thesis, University of Missouri – Columbia. Accessed March 06, 2021. http://hdl.handle.net/10355/45772.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ostdiek, Allison M. “Development of a nanomaterial-tissue patch for vascular and cardiac reconstruction.” 2014. Web. 06 Mar 2021.

Vancouver:

Ostdiek AM. Development of a nanomaterial-tissue patch for vascular and cardiac reconstruction. [Internet] [Thesis]. University of Missouri – Columbia; 2014. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/10355/45772.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ostdiek AM. Development of a nanomaterial-tissue patch for vascular and cardiac reconstruction. [Thesis]. University of Missouri – Columbia; 2014. Available from: http://hdl.handle.net/10355/45772

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

North Carolina State University

20. Moghe, Ajit Keshav. Study and Characterization of Small Diameter Woven Tubular Fabrics.

Degree: MS, Textile Engineering, 2003, North Carolina State University

URL: http://www.lib.ncsu.edu/resolver/1840.16/1468

► Woven tubular structures have been used since 1950s as vascular grafts in repairing damaged or diseased arteries and veins. The grafts for large caliber blood… (more)

Subjects/Keywords: medical textiles; textile biomaterials; artificial arteries; vascular grafts; arterial grafts; tubular structures

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APA (6^th Edition):

Moghe, A. K. (2003). Study and Characterization of Small Diameter Woven Tubular Fabrics. (Thesis). North Carolina State University. Retrieved from http://www.lib.ncsu.edu/resolver/1840.16/1468

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Moghe, Ajit Keshav. “Study and Characterization of Small Diameter Woven Tubular Fabrics.” 2003. Thesis, North Carolina State University. Accessed March 06, 2021. http://www.lib.ncsu.edu/resolver/1840.16/1468.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Moghe, Ajit Keshav. “Study and Characterization of Small Diameter Woven Tubular Fabrics.” 2003. Web. 06 Mar 2021.

Vancouver:

Moghe AK. Study and Characterization of Small Diameter Woven Tubular Fabrics. [Internet] [Thesis]. North Carolina State University; 2003. [cited 2021 Mar 06]. Available from: http://www.lib.ncsu.edu/resolver/1840.16/1468.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Moghe AK. Study and Characterization of Small Diameter Woven Tubular Fabrics. [Thesis]. North Carolina State University; 2003. Available from: http://www.lib.ncsu.edu/resolver/1840.16/1468

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Texas A&M University

21. Bulick, Allen. Systematic Investigation of Hydrogel Material Properties on Cell Responses for Vocal Fold and Vascular Graft Tissue Engineering.

Degree: PhD, Chemical Engineering, 2010, Texas A&M University

URL: http://hdl.handle.net/1969.1/ETD-TAMU-2009-08-3249

► The research presented here deals with synthetic materials for application in tissue engineering, primarily poly(ethylene glycol) (PEG) and poly(dimethyl siloxane)star (PDMS)star. Tissue engineering seeks to… (more)

▼ The research presented here deals with synthetic materials for application in tissue engineering, primarily poly(ethylene glycol) (PEG) and poly(dimethyl siloxane)star (PDMS)star. Tissue engineering seeks to repair or replace damaged tissue through implantation of cell encapsulated in an artificial scaffold. Cell differentiation and extracellular matrix (ECM) deposition can be influenced through a wide variety of in vitro culture techniques including biochemical stimuli, cell-cell interactions, mechanical conditioning and scaffold physical properties. In order to systematically optimize in vitro conditions for tissue engineering experiments, the individual effects of these different components must be studied. PEG hydrogels are a suitable scaffold for this because of their biocompatibility and biological "blank slate" nature. This dissertation presents data investigating: the effects of glycosaminoglycans (GAGs) as biochemical stimuli on pig vocal fold fibroblasts (PVFfs); the effects of mechanical conditioning and cell-cell interactions on smooth muscle cells (SMCs); and the effects of scaffold physical properties on SMCs. Results show that GAGs influence PVFf behavior and are an important component in scaffold design. Hyaluronic acid (HA) formulations showed similar production in collagen I and III as well as reduced levels of smooth muscle a-actin (SMa-actin), while chondroitin sulfate (CSC) and heparin sulfate showed enriched collagen III environments with enhanced expression of SMa-actin. A physiological flow system was developed to give comprehensive control over in vitro mechanical conditioning on TEVGs. Experiments performed on SMCs involved creating multi-layered TEVGs to mimic natural vascular tissue. Constructs subjected to mechanical conditioning with an endothelial cell (EC) layer showed enhanced expression of SMC differentiation markers calponin h1 and myocardin and enhanced deposition of elastin. Consistent with other studies, EC presence diminished overall collagen production and collagen I, specifically. Novel PDMSstar-PEG hydrogels were studied to investigate the effects of inorganic content on mesenchymal stem cell differentiation for use in TEVGs. Results agree with previous observations showing that a ratio of 5:95 PDMSstar: PEG by weight enhances SMC differentiation markers; however, statistically significant conclusions could not be made. By studying and optimizing in vitro culture conditions including scaffold properties, mechanical conditioning and multi-layered cell-cell interactions, TEVGs can be designed to maximize SMC differentiation and ECM production. Advisors/Committee Members: Hahn, Mariah (advisor), Pishko, Michael (committee member), Grunlan, Melissa (committee member), Cheng, Zhengdong (committee member).

Subjects/Keywords: Tissue Engineering; Smooth Muscle Cells; Vocal Fold; Vascular Grafts

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APA (6^th Edition):

Bulick, A. (2010). Systematic Investigation of Hydrogel Material Properties on Cell Responses for Vocal Fold and Vascular Graft Tissue Engineering. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-2009-08-3249

Chicago Manual of Style (16^th Edition):

Bulick, Allen. “Systematic Investigation of Hydrogel Material Properties on Cell Responses for Vocal Fold and Vascular Graft Tissue Engineering.” 2010. Doctoral Dissertation, Texas A&M University. Accessed March 06, 2021. http://hdl.handle.net/1969.1/ETD-TAMU-2009-08-3249.

MLA Handbook (7^th Edition):

Bulick, Allen. “Systematic Investigation of Hydrogel Material Properties on Cell Responses for Vocal Fold and Vascular Graft Tissue Engineering.” 2010. Web. 06 Mar 2021.

Vancouver:

Bulick A. Systematic Investigation of Hydrogel Material Properties on Cell Responses for Vocal Fold and Vascular Graft Tissue Engineering. [Internet] [Doctoral dissertation]. Texas A&M University; 2010. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2009-08-3249.

Council of Science Editors:

Bulick A. Systematic Investigation of Hydrogel Material Properties on Cell Responses for Vocal Fold and Vascular Graft Tissue Engineering. [Doctoral Dissertation]. Texas A&M University; 2010. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2009-08-3249

North Carolina State University

22. Agrawal, Pankaj. Design And Study of Biodegradable Small Diameter Vascular Grafts.

Degree: MS, Textile Engineering, 2003, North Carolina State University

URL: http://www.lib.ncsu.edu/resolver/1840.16/1362

► In this research, the focus has been on designing and producing small diameter woven grafts having a biodegradable material. Woven tubes in diameters ranging from… (more)

Subjects/Keywords: Woven Vascular Grafts; medical textiles; Polyglactin 910; PLGA; Biodegradable

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APA (6^th Edition):

Agrawal, P. (2003). Design And Study of Biodegradable Small Diameter Vascular Grafts. (Thesis). North Carolina State University. Retrieved from http://www.lib.ncsu.edu/resolver/1840.16/1362

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Agrawal, Pankaj. “Design And Study of Biodegradable Small Diameter Vascular Grafts.” 2003. Thesis, North Carolina State University. Accessed March 06, 2021. http://www.lib.ncsu.edu/resolver/1840.16/1362.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Agrawal, Pankaj. “Design And Study of Biodegradable Small Diameter Vascular Grafts.” 2003. Web. 06 Mar 2021.

Vancouver:

Agrawal P. Design And Study of Biodegradable Small Diameter Vascular Grafts. [Internet] [Thesis]. North Carolina State University; 2003. [cited 2021 Mar 06]. Available from: http://www.lib.ncsu.edu/resolver/1840.16/1362.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Agrawal P. Design And Study of Biodegradable Small Diameter Vascular Grafts. [Thesis]. North Carolina State University; 2003. Available from: http://www.lib.ncsu.edu/resolver/1840.16/1362

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

23. HE WEI. Electrospun Nanofibre Scaffolds for Tissue Engineered Small-Diameter Vascular Grafts.

Degree: 2008, National University of Singapore

URL: http://scholarbank.nus.edu.sg/handle/10635/16104

Subjects/Keywords: electrospinning; nanofibers; tissue engineering; vascular grafts. scaffolds

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APA (6^th Edition):

WEI, H. (2008). Electrospun Nanofibre Scaffolds for Tissue Engineered Small-Diameter Vascular Grafts. (Thesis). National University of Singapore. Retrieved from http://scholarbank.nus.edu.sg/handle/10635/16104

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

WEI, HE. “Electrospun Nanofibre Scaffolds for Tissue Engineered Small-Diameter Vascular Grafts.” 2008. Thesis, National University of Singapore. Accessed March 06, 2021. http://scholarbank.nus.edu.sg/handle/10635/16104.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

WEI, HE. “Electrospun Nanofibre Scaffolds for Tissue Engineered Small-Diameter Vascular Grafts.” 2008. Web. 06 Mar 2021.

Vancouver:

WEI H. Electrospun Nanofibre Scaffolds for Tissue Engineered Small-Diameter Vascular Grafts. [Internet] [Thesis]. National University of Singapore; 2008. [cited 2021 Mar 06]. Available from: http://scholarbank.nus.edu.sg/handle/10635/16104.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

WEI H. Electrospun Nanofibre Scaffolds for Tissue Engineered Small-Diameter Vascular Grafts. [Thesis]. National University of Singapore; 2008. Available from: http://scholarbank.nus.edu.sg/handle/10635/16104

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Texas Tech University

24. Chen, Jyh-herng. Modeling and optimization of heparinized collagenous surfaces.

Degree: Chemical Engineering, 1987, Texas Tech University

URL: http://hdl.handle.net/2346/17939

► The primary objective of this research is to determine the optimal conditions of heparin immobilization on collagen powder by varying pretreatment methods, pH of reaction… (more)

Subjects/Keywords: Collagen; Blood vessel prosthesis; Vascular grafts; Heparin

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APA (6^th Edition):

Chen, J. (1987). Modeling and optimization of heparinized collagenous surfaces. (Thesis). Texas Tech University. Retrieved from http://hdl.handle.net/2346/17939

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Chen, Jyh-herng. “Modeling and optimization of heparinized collagenous surfaces.” 1987. Thesis, Texas Tech University. Accessed March 06, 2021. http://hdl.handle.net/2346/17939.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Chen, Jyh-herng. “Modeling and optimization of heparinized collagenous surfaces.” 1987. Web. 06 Mar 2021.

Vancouver:

Chen J. Modeling and optimization of heparinized collagenous surfaces. [Internet] [Thesis]. Texas Tech University; 1987. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/2346/17939.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Chen J. Modeling and optimization of heparinized collagenous surfaces. [Thesis]. Texas Tech University; 1987. Available from: http://hdl.handle.net/2346/17939

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Arizona

25. Cardinal, Kristen O'Halloran. Development and Utilization of a Tissue Engineered Blood Vessel Mimic to Assess the Neointimal Response to Intravascular Stents .

Degree: 2007, University of Arizona

URL: http://hdl.handle.net/10150/195387

► The use of intravascular stents to restore blood flow through restricted vessels in patients with coronary artery disease has become the preferred method for treating… (more)

▼ The use of intravascular stents to restore blood flow through restricted vessels in patients with coronary artery disease has become the preferred method for treating a variety of lesion locations and pathologies. As new stent configurations and coatings are developed, a great need exists for high-throughput preclinical evaluation techniques that can interface human tissue with three-dimensional devices. Thus, the goals of this dissertation research were 1) to develop an in vitro blood vessel mimic composed of human cells for preclinical evaluation of intravascular devices, and 2) to utilize the mimic to assess neointimal responses to implanted stents.Experiments in support of these goals were broken into four specific aims. The first aim was to develop an in vitro human blood vessel mimic based on techniques for creating tissue engineered vascular grafts. The second aim was to determine the feasibility of utilizing this vessel mimic for bare metal stent evaluation. The third aim was to use the in vitro vessel to evaluate the cellular response to protein-coated stents. The fourth aim was to take advantage of the ability to control the in vitro vessel environment in order to evaluate the effect of shear rate on the neointimal response to implanted stents.Human blood vessel mimics were created by sodding fat-derived microvascular endothelial cells onto expanded polytetrafluoroethylene grafts and cultivating the vessels in bioreactor systems. This resulted in the development of a luminal lining of endothelial cells with sub-endothelial smooth muscle and mesenchymal cells. Deployment and assessment of bare metal stents within blood vessel mimics supported the feasibility of using the model for stent evaluation, and demonstrated that cell coverage of the device surface could be observed and measured. Protein-modified stents were created by submerging devices in enriched medium, and following implantation in the blood vessel mimic exhibited increased cell coverage and increased tissue thickness as compared with bare metal stents. Finally, an increase in shear rate lead to decreased neointimal coverage of implanted bare metal and modified stents. Overall, this dissertation demonstrates that in vitro human blood vessel mimics can be created and utilized for preclinical device evaluation. Advisors/Committee Members: Williams, Stuart K. (committeemember), Hoying, James (committeemember), Riley, Mark (committeemember), Hossainy, Syed (committeemember), Berman, Scott (committeemember).

Subjects/Keywords: tissue engineered vascular grafts; stents; endothelial cells

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APA (6^th Edition):

Cardinal, K. O. (2007). Development and Utilization of a Tissue Engineered Blood Vessel Mimic to Assess the Neointimal Response to Intravascular Stents . (Doctoral Dissertation). University of Arizona. Retrieved from http://hdl.handle.net/10150/195387

Chicago Manual of Style (16^th Edition):

Cardinal, Kristen O'Halloran. “Development and Utilization of a Tissue Engineered Blood Vessel Mimic to Assess the Neointimal Response to Intravascular Stents .” 2007. Doctoral Dissertation, University of Arizona. Accessed March 06, 2021. http://hdl.handle.net/10150/195387.

MLA Handbook (7^th Edition):

Cardinal, Kristen O'Halloran. “Development and Utilization of a Tissue Engineered Blood Vessel Mimic to Assess the Neointimal Response to Intravascular Stents .” 2007. Web. 06 Mar 2021.

Vancouver:

Cardinal KO. Development and Utilization of a Tissue Engineered Blood Vessel Mimic to Assess the Neointimal Response to Intravascular Stents . [Internet] [Doctoral dissertation]. University of Arizona; 2007. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/10150/195387.

Council of Science Editors:

Cardinal KO. Development and Utilization of a Tissue Engineered Blood Vessel Mimic to Assess the Neointimal Response to Intravascular Stents . [Doctoral Dissertation]. University of Arizona; 2007. Available from: http://hdl.handle.net/10150/195387

Georgia Tech

26. Costello, James Robert. Effect of Material Properties and Hemodynamics on the Healing of Vascular Grafts in baboons.

Degree: PhD, Biomedical Engineering, 2004, Georgia Tech

URL: http://hdl.handle.net/1853/5226

► Each year, more than one million prosthetic vascular grafts are implanted. Well-over 50 % of these artificial vessels are of the small caliber variety with… (more)

▼ Each year, more than one million prosthetic vascular grafts are implanted. Well-over 50 % of these artificial vessels are of the small caliber variety with an inner diameter less than or equal to 10 mm. The challenge rests in implanting these synthetic substitutes into a hemodynamic environment with a high downstream resistance and low rates of flow. Over the course of four interrelated studies, we investigated the healing properties of small caliber prosthetic vascular grafts. All of these studies were conducted using baboons. First, we documented the difference in healing response between three different types of vascular grafts: (1) autologous artery (2) allogeneic vessel (3) prosthetic ePTFE. This comparison furnished an important model of graft healing. Proliferating endothelial cells were localized to the top 10 % of the neointima, while the proliferating smooth muscle cells were identified within the lower 10 % of the neointima. Secondly, we examined the effects of changing a prosthetic grafts material properties and how that change impacts healing of the grafts surface. These ultrastructural changes were introduced by radially stretching a porous 60 mm ePTFE vascular graft. Radially stretching the graft material decreased the void fraction, reduced the potential for transmural ingrowth, and changed the healing characteristics of the implanted vessels. Thirdly, we investigated the effect of a changing hemodynamic environment upon the healing of a vascular graft with uniform material properties. The changing hemodynamics were generated with a stenotic model. Under sub-acute conditions, an inverse relationship failed to exist between intimal thickening and wall shear stress. Lastly, the details of this hemodynamic environment were documented with computational fluid dynamics (CFD). The computational grids were constructed using three sets of geometric information: (1) incorporating the ideal material dimensions of the implanted vessel (2) utilizing contour information from pressure-perfused histologic cross-sections (3) applying geometric information form detailed MRI imaging. MRI imaging information provided the best description of the vessels hemodynamic environment. With this computational information, correlations were made between the intimal thickening and hemodynamic parameters. Advisors/Committee Members: Don P. Giddens (Committee Co-Chair), Stephen R. Hanson (Committee Co-Chair), Changyi Chen (Committee Member), Hanjoong Jo (Committee Member), Robert Nerem (Committee Member).

Subjects/Keywords: CFD; MRI imaging; Hemodynamics; ePTFE; Prosthetic vascular grafts

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APA (6^th Edition):

Costello, J. R. (2004). Effect of Material Properties and Hemodynamics on the Healing of Vascular Grafts in baboons. (Doctoral Dissertation). Georgia Tech. Retrieved from http://hdl.handle.net/1853/5226

Chicago Manual of Style (16^th Edition):

Costello, James Robert. “Effect of Material Properties and Hemodynamics on the Healing of Vascular Grafts in baboons.” 2004. Doctoral Dissertation, Georgia Tech. Accessed March 06, 2021. http://hdl.handle.net/1853/5226.

MLA Handbook (7^th Edition):

Costello, James Robert. “Effect of Material Properties and Hemodynamics on the Healing of Vascular Grafts in baboons.” 2004. Web. 06 Mar 2021.

Vancouver:

Costello JR. Effect of Material Properties and Hemodynamics on the Healing of Vascular Grafts in baboons. [Internet] [Doctoral dissertation]. Georgia Tech; 2004. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/1853/5226.

Council of Science Editors:

Costello JR. Effect of Material Properties and Hemodynamics on the Healing of Vascular Grafts in baboons. [Doctoral Dissertation]. Georgia Tech; 2004. Available from: http://hdl.handle.net/1853/5226

Georgia Tech

27. Kumar, Vivek Ashok. Design and evaluation of scaffolds for arterial grafts using extracellular matrix based materials.

Degree: PhD, Biomedical Engineering, 2011, Georgia Tech

URL: http://hdl.handle.net/1853/45869

► For small diameter (<6 mm) blood vessel replacements, lack of collaterals and vascular disease preclude homografts; while synthetic analogs, ePTFE, expanded polytetrafluoroethylene, and PET, polyethyleneterephathalate,… (more)

Subjects/Keywords: Rat aortic interposition; Rat tail tendon; Vascular; Elastin; Collagen; Soft tissue; Blood vessels; Tissue engineering; Regenerative medicine; Arterial grafts; Tissue scaffolds

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kumar, V. A. (2011). Design and evaluation of scaffolds for arterial grafts using extracellular matrix based materials. (Doctoral Dissertation). Georgia Tech. Retrieved from http://hdl.handle.net/1853/45869

Chicago Manual of Style (16^th Edition):

Kumar, Vivek Ashok. “Design and evaluation of scaffolds for arterial grafts using extracellular matrix based materials.” 2011. Doctoral Dissertation, Georgia Tech. Accessed March 06, 2021. http://hdl.handle.net/1853/45869.

MLA Handbook (7^th Edition):

Kumar, Vivek Ashok. “Design and evaluation of scaffolds for arterial grafts using extracellular matrix based materials.” 2011. Web. 06 Mar 2021.

Vancouver:

Kumar VA. Design and evaluation of scaffolds for arterial grafts using extracellular matrix based materials. [Internet] [Doctoral dissertation]. Georgia Tech; 2011. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/1853/45869.

Council of Science Editors:

Kumar VA. Design and evaluation of scaffolds for arterial grafts using extracellular matrix based materials. [Doctoral Dissertation]. Georgia Tech; 2011. Available from: http://hdl.handle.net/1853/45869

University of Maryland

28. Melchiorri, Anthony John. Engineering Biodegradable Vascular Scaffolds for Congenital Heart Disease.

Degree: Bioengineering, 2015, University of Maryland

URL: http://hdl.handle.net/1903/17334

► The most common birth defects worldwide are congenital heart defects. To treat these malformations in a child’s cardiovascular system, synthetic grafts have been used as… (more)

▼ The most common birth defects worldwide are congenital heart defects. To treat these malformations in a child’s cardiovascular system, synthetic grafts have been used as a primary intervention. However, current grafts suffer from deficiencies such as minimal biological compatibility, inability to grow and adapt, and high failure rates. Additionally, the grafts are not customized to the patient, which may lead to graft failure given that defects may vary significantly from patient to patient. The work presented here aims to adapt tissue engineering paradigms to develop customizable vascular grafts for congenital heart defects using to reduce the long-term risk and the number of surgeries experienced by patients. The first component of this research focuses on solvent-cast vascular grafts. This system of fabrication was used to explore how various strategies and graft modifications affect the graft’s performance in vivo. Grafts were fabricated with the mechanical properties necessary to withstand the stresses of a physiological environment and support neotissue formation. To improve tissue formation, the grafts were modified with bioactive molecules to improve vascular tissue growth. In addition, the grafts were combined with a tissue perfusion bioreactor. The bioreactor applied fluid flow to support cell seeding, differentiation, and growth of endothelial progenitor cells on the grafts, demonstrating a robust strategy for tissue formation prior to implantation. The second component of this research centers on the development of a biomaterial for 3D printing. 3D printing offers unparalleled customizability, as a graft can be designed based on medical images of a patient, tested via computer modeling, and then printed for implantation. A resin was developed consisting to produce grafts that were mechanically compatible with native blood vessels and maintained patency and tissue formation six months after implantation. The library of 3D printed vascular graft materials was also expanded by creating a novel copolymer resins, which varied in mechanical properties and degradation profiles. In addition, the concepts and strategies of biofunctionalization developed in the solvent-cast vascular grafts can be combined with the 3D printed graft strategies. Grafts designed, printed, and modified using these combinatorial approaches can greatly improve the long-term outcomes of treating congenital heart disease. Advisors/Committee Members: Fisher, John P (advisor).

Subjects/Keywords: Biomedical engineering; Medicine; Materials Science; 3D printing; biomaterials; cardiovascular materials; congenital heart disease; polymers; vascular grafts

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Melchiorri, A. J. (2015). Engineering Biodegradable Vascular Scaffolds for Congenital Heart Disease. (Thesis). University of Maryland. Retrieved from http://hdl.handle.net/1903/17334

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Melchiorri, Anthony John. “Engineering Biodegradable Vascular Scaffolds for Congenital Heart Disease.” 2015. Thesis, University of Maryland. Accessed March 06, 2021. http://hdl.handle.net/1903/17334.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Melchiorri, Anthony John. “Engineering Biodegradable Vascular Scaffolds for Congenital Heart Disease.” 2015. Web. 06 Mar 2021.

Vancouver:

Melchiorri AJ. Engineering Biodegradable Vascular Scaffolds for Congenital Heart Disease. [Internet] [Thesis]. University of Maryland; 2015. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/1903/17334.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Melchiorri AJ. Engineering Biodegradable Vascular Scaffolds for Congenital Heart Disease. [Thesis]. University of Maryland; 2015. Available from: http://hdl.handle.net/1903/17334

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Georgia Tech

29. Williams, Chrysanthi. Perfusion bioreactor for tissue-engineered blood vessels.

Degree: PhD, Bioengineering, 2003, Georgia Tech

URL: http://hdl.handle.net/1853/9455

Subjects/Keywords: Tissue engineering; Bioreactors Design and construction; Vascular grafts; Vascular grafts; Tissue engineering; Bioreactors Design and construction

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Williams, C. (2003). Perfusion bioreactor for tissue-engineered blood vessels. (Doctoral Dissertation). Georgia Tech. Retrieved from http://hdl.handle.net/1853/9455

Chicago Manual of Style (16^th Edition):

Williams, Chrysanthi. “Perfusion bioreactor for tissue-engineered blood vessels.” 2003. Doctoral Dissertation, Georgia Tech. Accessed March 06, 2021. http://hdl.handle.net/1853/9455.

MLA Handbook (7^th Edition):

Williams, Chrysanthi. “Perfusion bioreactor for tissue-engineered blood vessels.” 2003. Web. 06 Mar 2021.

Vancouver:

Williams C. Perfusion bioreactor for tissue-engineered blood vessels. [Internet] [Doctoral dissertation]. Georgia Tech; 2003. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/1853/9455.

Council of Science Editors:

Williams C. Perfusion bioreactor for tissue-engineered blood vessels. [Doctoral Dissertation]. Georgia Tech; 2003. Available from: http://hdl.handle.net/1853/9455

30. Munoz Pinto, Dany 1981-. Hybrid Polyethylene Glycol Hydrogels for Tissue Engineering Applications.

Degree: PhD, Chemical Engineering, 2012, Texas A&M University

URL: http://hdl.handle.net/1969.1/149213

► Currently, organ transplant procedures are insufficient to address the needs of the number of patients that suffer of organ failure related disease. In the United… (more)

▼ Currently, organ transplant procedures are insufficient to address the needs of the number of patients that suffer of organ failure related disease. In the United States alone, only around 19% of the patients are able to get an organ transplant surgery and 25% die while waiting for a suitable donor. Tissue engineering (TE) has emerged as an alternative to organ transplant; thus, the aim of the present study was to validate a poly(ethylene glycol) diacrylate (PEG-DA) hydrogel system as a model for material scaffolding in TE applications. This work explores the influence of scaffold material properties on cell behavior. Specifically, scaffold modulus, mesh size, and biochemical stimuli were characterized and their influence on cell response was analyzed at the biochemical, histological and microenvironmental levels. Three different TE targets were evaluated: vocal fold restoration, vascular grafts and osteochondral applications. Vocal fold fibroblast (VFF) phenotype and extracellular matrix (ECM) production were impacted by initial scaffold mesh size and modulus. The results showed increasing levels of SM-α-actin and collagen production with decreasing initial mesh size/increasing initial modulus, which indicated that VFFs were induced to take an undesirable myofibroblast-like phenotype. In addition, it was possible to preserve VFF phenotype in long-term cultured hydrogels containing high molecular weight hyaluronan (HAHMW). On the other hand, regarding vascular graft applications, smooth muscle cell (SMC) phenotype was enhanced by increasing scaffold mesh size and modulus. Finally, the effect of scaffold inorganic content (siloxane) on rat osteoblasts and mouse mesenchymal stem cells was evaluated. Interestingly, the impact of inorganic content on cell differentiation seemed to be highly dependent on the initial cell state. Specifically, mature osteoblasts underwent transdifferentiation into chondrocyte-like cells with increasing inorganic content. However, Mesenchymal stem cells appeared to be preferentially driven toward osteoblast-like cells with an associated increase in osteocalcin and collagen type I production. Advisors/Committee Members: Hahn, Mariah S (advisor), Grunlan, Melissa (committee member), Pishko, Michael (committee member), Ugaz, Victor (committee member).

Subjects/Keywords: Bone tissue engineering; Vascular grafts; Tissue engineering; Hydrogel

…small diameter vascular graft, and bone regeneration. Cell responses were analyzed at the…

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Munoz Pinto, D. 1. (2012). Hybrid Polyethylene Glycol Hydrogels for Tissue Engineering Applications. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/149213

Chicago Manual of Style (16^th Edition):

Munoz Pinto, Dany 1981-. “Hybrid Polyethylene Glycol Hydrogels for Tissue Engineering Applications.” 2012. Doctoral Dissertation, Texas A&M University. Accessed March 06, 2021. http://hdl.handle.net/1969.1/149213.

MLA Handbook (7^th Edition):

Munoz Pinto, Dany 1981-. “Hybrid Polyethylene Glycol Hydrogels for Tissue Engineering Applications.” 2012. Web. 06 Mar 2021.

Vancouver:

Munoz Pinto D1. Hybrid Polyethylene Glycol Hydrogels for Tissue Engineering Applications. [Internet] [Doctoral dissertation]. Texas A&M University; 2012. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/1969.1/149213.

Council of Science Editors:

Munoz Pinto D1. Hybrid Polyethylene Glycol Hydrogels for Tissue Engineering Applications. [Doctoral Dissertation]. Texas A&M University; 2012. Available from: http://hdl.handle.net/1969.1/149213

Open Access Theses and Dissertations