subject:(VP24)

1. ZHUANG YING. Structural studies on nucleocapsid proteins of shrimp white spot syndrome virus.

Degree: 2004, National University of Singapore

URL: http://scholarbank.nus.edu.sg/handle/10635/14163

Subjects/Keywords: WSSV; Capsid Protein; VP15; VP24

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

YING, Z. (2004). Structural studies on nucleocapsid proteins of shrimp white spot syndrome virus. (Thesis). National University of Singapore. Retrieved from http://scholarbank.nus.edu.sg/handle/10635/14163

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

YING, ZHUANG. “Structural studies on nucleocapsid proteins of shrimp white spot syndrome virus.” 2004. Thesis, National University of Singapore. Accessed January 19, 2021. http://scholarbank.nus.edu.sg/handle/10635/14163.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

YING, ZHUANG. “Structural studies on nucleocapsid proteins of shrimp white spot syndrome virus.” 2004. Web. 19 Jan 2021.

Vancouver:

YING Z. Structural studies on nucleocapsid proteins of shrimp white spot syndrome virus. [Internet] [Thesis]. National University of Singapore; 2004. [cited 2021 Jan 19]. Available from: http://scholarbank.nus.edu.sg/handle/10635/14163.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

YING Z. Structural studies on nucleocapsid proteins of shrimp white spot syndrome virus. [Thesis]. National University of Singapore; 2004. Available from: http://scholarbank.nus.edu.sg/handle/10635/14163

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

2. Page, Audrey. Etude de la modulation de la réponse cellulaire au stress oxydatif par les protéines VP24 des virus Marburg et Ebola : Study of modulation of anti-oxidative cellular response by VP24 proteins of Marburgvirus and Ebolavirus.

Degree: Docteur es, Sciences de la vie, 2012, Lyon, École normale supérieure

URL: http://www.theses.fr/2012ENSL0706

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Les virus Ebola (EBOV) et Marburg (MARV) causent des fièvres hémorragiques chez les primates, y compris l’homme. Le taux de létalité peut atteindre 90% et… (more)

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Les virus Ebola (EBOV) et Marburg (MARV) causent des fièvres hémorragiques chez les primates, y compris l’homme. Le taux de létalité peut atteindre 90% et il n’existe ni vaccin ni traitement contre ces virus. En raison de leurs caractéristiques moléculaires communes, EBOV et MARV sont regroupés au sein de la famille des Filoviridae. Le virion est composé de 7 protéines, dont la VP24, qui joue un rôle important dans l’assemblage et la condensation des nucléocapsides, et pour EBOV, elle est également responsable de l’inhibition de la réponse à l’IFN. Des mutations dans la séquence protéique de VP24 sont impliquées dans le processus d’adaptation chez un nouvel hôte. La protéine VP24 d’EBOV est donc multifonctionnelle. Pour MARV, cette protéine ne semble pas porter les fonctions décrites pour la VP24 d’EBOV. Afin de comprendre le rôle de la VP24 de MARV, nous avons identifié ses partenaires cellulaires par un crible double-hybride en levures. Nous avons mis en évidence l’interaction entre Keap1 et la VP24 de MARV, et confirmé ce résultat en cellules mammifères. Keap1 est une protéine impliquée dans le contrôle de la réponse au stress oxydatif, car elle inhibe le facteur de transcription Nrf2, qui régule l’expression d’enzymes impliquées dans la réduction des ERO. Nos résultats montrent que le domaine de Keap1 liant la VP24 est le même que celui liant Nrf2, et que la VP24 de MARV active Nrf2 pour la synthèse de molécules anti-oxydantes. Nous avons enfin évalué l’impact de la VP24 de MARV sur ERR, une autre cible de Keap1, et mesuré l’activité Nrf2 au cours de l’infection par EBOV. Nos résultats montrent des effets opposés des VP24 d’ EBOV et de MARV sur l’activité de Nrf2.

Ebola (EBOV) and Marburgvirus (MARV) are responsible for severe hemorrhagic syndrome in primates, including humans. The lethality rate can reach 90%, and no vaccine or treatment is available to counteract these diseases. EBOV and MARV have similar genomic organization and thus are placed in a distinct family, Filoviridae. VP24 is one of the 7 structural proteins which form the virion and has been shown to play an important role in assembly and condensation of viral nucleocapsids. VP24 of EBOV is responsible for prevention of cellular response to IFN. Mutations in EBOV VP24 gene are necessary for the adaptation to a new host. EBOV VP24 thus acts as a multifunctional factor. Available data suggest that MARV VP24 is not implicated in either the counteraction of IFN response, or in the adaptation process. In order to discover new functions for VP24 of MARV, we searched for its interaction with cellular proteins, using a yeast-double hybrid approach. We discovered an interaction between MARV VP24 and Keap1 protein and further confirmed this interaction in mammalian cells. Keap1 is a cellular protein involved in intracellular detection of Reactive Oxygen Species (ROS) and in the control of oxidative stress response. It inhibits the Nrf2 transcription factor, which regulates expression of antioxidant enzymes. Our results indicate that Keap1 binding domain…

Advisors/Committee Members: Volchkov, Viktor (thesis director).

Subjects/Keywords: Filovirus; Virus Marburg; Virus Ebola; Keap1; Nrf2; Stress oxydatif; Réponse anti-oxydante; VP24; Filovirus; Marburgvirus; Ebolavirus; Keap1; Nrf2; Oxidative stress; Anti-oxidative response; VP24

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Page, A. (2012). Etude de la modulation de la réponse cellulaire au stress oxydatif par les protéines VP24 des virus Marburg et Ebola : Study of modulation of anti-oxidative cellular response by VP24 proteins of Marburgvirus and Ebolavirus. (Doctoral Dissertation). Lyon, École normale supérieure. Retrieved from http://www.theses.fr/2012ENSL0706

Chicago Manual of Style (16^th Edition):

Page, Audrey. “Etude de la modulation de la réponse cellulaire au stress oxydatif par les protéines VP24 des virus Marburg et Ebola : Study of modulation of anti-oxidative cellular response by VP24 proteins of Marburgvirus and Ebolavirus.” 2012. Doctoral Dissertation, Lyon, École normale supérieure. Accessed January 19, 2021. http://www.theses.fr/2012ENSL0706.

MLA Handbook (7^th Edition):

Page, Audrey. “Etude de la modulation de la réponse cellulaire au stress oxydatif par les protéines VP24 des virus Marburg et Ebola : Study of modulation of anti-oxidative cellular response by VP24 proteins of Marburgvirus and Ebolavirus.” 2012. Web. 19 Jan 2021.

Vancouver:

Page A. Etude de la modulation de la réponse cellulaire au stress oxydatif par les protéines VP24 des virus Marburg et Ebola : Study of modulation of anti-oxidative cellular response by VP24 proteins of Marburgvirus and Ebolavirus. [Internet] [Doctoral dissertation]. Lyon, École normale supérieure; 2012. [cited 2021 Jan 19]. Available from: http://www.theses.fr/2012ENSL0706.

Council of Science Editors:

Page A. Etude de la modulation de la réponse cellulaire au stress oxydatif par les protéines VP24 des virus Marburg et Ebola : Study of modulation of anti-oxidative cellular response by VP24 proteins of Marburgvirus and Ebolavirus. [Doctoral Dissertation]. Lyon, École normale supérieure; 2012. Available from: http://www.theses.fr/2012ENSL0706

Uppsala University

3. Obaid, Marina. The Expression, Purification and Characterization of Ebola Virion Protein 24 and Karyopherin Alpha 5.

Degree: Women's and Children's Health, 2018, Uppsala University

URL: http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-355149

► Ebolavirus (EBOV) is a single stranded RNA virus that causes haemorrhagic fever in humans and other mammals. The EBOV encodes 7 proteins, NP, L,… (more)

Subjects/Keywords: Ebolavirus; filovirus; VP24; KPNA5; IMAC; Cell and Molecular Biology; Cell- och molekylärbiologi

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Obaid, M. (2018). The Expression, Purification and Characterization of Ebola Virion Protein 24 and Karyopherin Alpha 5. (Thesis). Uppsala University. Retrieved from http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-355149

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Obaid, Marina. “The Expression, Purification and Characterization of Ebola Virion Protein 24 and Karyopherin Alpha 5.” 2018. Thesis, Uppsala University. Accessed January 19, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-355149.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Obaid, Marina. “The Expression, Purification and Characterization of Ebola Virion Protein 24 and Karyopherin Alpha 5.” 2018. Web. 19 Jan 2021.

Vancouver:

Obaid M. The Expression, Purification and Characterization of Ebola Virion Protein 24 and Karyopherin Alpha 5. [Internet] [Thesis]. Uppsala University; 2018. [cited 2021 Jan 19]. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-355149.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Obaid M. The Expression, Purification and Characterization of Ebola Virion Protein 24 and Karyopherin Alpha 5. [Thesis]. Uppsala University; 2018. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-355149

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

4. F. Dapiaggi. MOLECULAR MODELING OF EBOLA VIRUS INHIBITORS.

Degree: 2018, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/545872

► In this PhD thesis computational methods have been employed in order to study different biologically relevant systems. In the first part of the thesis two… (more)

▼ In this PhD thesis computational methods have been employed in order to study different biologically relevant systems. In the first part of the thesis two Ebola virus proteins were studied, namely Viral Protein 24 (VP24) and Viral Protein 35 (VP35), responsible for the inhibition of the immune response . After a brief theoretical introduction to the main computational methods employed in the thesis, a study of VP35 in complex with small organic molecules is presented. These compounds are able to inhibit the interaction between VP35 and viral nucleoprotein. This study confirms the experimental findings highlighting new important key interactions between the protein the inhibitors. Moreover, an Essential Dynamics analysis points out an interesting collective motion of the apo-form that is hindered by the presence of the ligands. Afterwards, the protein-protein interaction VP24-Karyopherin (KPNA) is studied. An atomistic analysis of the interactions at the interface leads to the design of a nonapeptide with VP24 binding capability. The peptide is derived from a KPNA subsequence and could potentially inhibit the VP24-KPNA interaction. Subsequently an analysis on the pockets present on VP24 surface in different solvents is performed. Once the most promising pocket has been located, a virtual screening on a subset of ZINC database is carried out, leading to the identification of few classes of molecules potentially able to bind VP24. Finally the effect of the osmolytes on VP24 protein structure is studied, pointing out how osmoprotectants and urea have opposite effects on the protein, the former stabilizing the folded state and the latter shifting the equilibrium to the denatured state. In the second part of the manuscript the study of the interaction of an antimicrobial peptide with a lipid membrane is presented. This work was carried out in the University of Groningen under the supervision of Prof. Siewert Jan Marrink in order to deepen the Coarse Grain method. Advisors/Committee Members: tutor: M. Sironi, co-tutor: S. Pieraccini, coordinatore: E. Licandro, LICANDRO, EMANUELA.

Subjects/Keywords: Ebola; Virus; Computational; Chemistry; Molecular dynamics; Free energy; MMPBSA; Docking; Coarse Grain; VP24; VP35; Virtual Screening; Osmolytes; Protein; Osmoprotectants; Denaturants; Inhibitors; Peptide; Settore CHIM/02 - Chimica Fisica

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Dapiaggi, F. (2018). MOLECULAR MODELING OF EBOLA VIRUS INHIBITORS. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/545872

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Dapiaggi, F.. “MOLECULAR MODELING OF EBOLA VIRUS INHIBITORS.” 2018. Thesis, Università degli Studi di Milano. Accessed January 19, 2021. http://hdl.handle.net/2434/545872.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Dapiaggi, F.. “MOLECULAR MODELING OF EBOLA VIRUS INHIBITORS.” 2018. Web. 19 Jan 2021.

Vancouver:

Dapiaggi F. MOLECULAR MODELING OF EBOLA VIRUS INHIBITORS. [Internet] [Thesis]. Università degli Studi di Milano; 2018. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/2434/545872.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Dapiaggi F. MOLECULAR MODELING OF EBOLA VIRUS INHIBITORS. [Thesis]. Università degli Studi di Milano; 2018. Available from: http://hdl.handle.net/2434/545872

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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