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1. Franco Mahecha, Olga Lucía. Estudio de la capacidad de antígenos del virus de la diarrea viral bovina (VDVB) de iniciar la respuesta inmune : interacción con las células dendríticas.

Degree: Ciencias Veterinarias, 2014, Universidad de Buenos Aires

Bovine viral diarrhea virus (BVDV) is a pathogen that affects cattle causing immune-suppression by killing\nlymphocytes and monocytes-macrophages. BVDV does not seem to interfere with dendritic cells (DC).\nHowever, the effects of the virus on DC during the first day of infection have not been studied. There is a\nneed of new vaccines to control this disease. Currently used inactivated vaccines are not protective, and\nthere are not recombinant vaccines available. One of the main issues in the evaluation of vaccine efficacy\nor even sero-conversion, is the difficulty of getting BVDV-free animals, especially in endemic areas.\nThese facts support the development of new-creative strategies to select vaccine antigens, reducing the\nnumber of animal trials needed. In this study we investigated the possibility of selecting vaccine antigens\nbased on their activity on DC. We also studied the interaction between BVDV and DC shortly after\ninfection, to bring information on the influence of these cells in the viral immunopathogenesis.\nWe developed a protocol to select antigens based on their capacity to activate DC in vitro, using both\nmurine and bovine DC. We evaluated several antigens: inactivated virus, the truncated form of the E2\nglycoprotein of the viral envelope (main target of the neutralizing response) expressed by a recombinant\nBaculovirus in insect cells ?Bt-E2?, and plasmids expressing t-E2. Activation of DC was measured by\ndetermining the up-regulation of co-stimulatory molecules and expression of pro-inflammatory cytokines.\nOnly the plasmids were able to induce a complete activation of the DC, indicating they might not require\nadjuvants; while Bt-E2 and the inactivated virus were unable to induce a complete activation of the DC.\nSimilar results were obtained when using murine or bovine DC.\nCandidate antigens were then tested for immunogenicity in mice, guinea-pigs and bovines. DNA vaccines\nwere no efficient inducing antibodies (Abs), while Bt-E2 formulated with adjuvants induced high levels of\nneutralizing Abs and, depending on the adjuvant, also cell-mediated immunity. The use of a nanoparticulated\nadjuvant, designed to activate DC, induced high levels of Abs with low antigen payload.\nLevels of neutralizing Abs were related to the amount of E2 in the vaccine, either recombinant or viral. In\nfact, Bt-E2 could be used an additive for the inactivated vaccine to achieve a protective E2-payload.\nBVDV was able to infect and replicate rapidly in the DC, and even though a cytopathic biotype was used,\nno effect on DC viability was observed. Infected DC could not get an activated phenotype during the first\nhours post-infection. We propose that by infecting DC and rescuing them from apoptosis, BVDV gets\naccess to the lymph-nodes were it can rapidly kill large numbers of T-cells present in the follicles.\nThe methodology developed in this study is particularly useful to compare different antigens of similar\nchemical composition. Selecting antigen candidates based on their capacity to activate DC is a… Advisors/Committee Members: Capozzo, Alejandra Victoria.

Subjects/Keywords: VDVB; Proteína E2; Células dendríticas; Bovinos; Virus de la diarrea vírica; Antígenos; Respuesta inmune; Células dendríticas; Inmunología

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Franco Mahecha, O. L. (2014). Estudio de la capacidad de antígenos del virus de la diarrea viral bovina (VDVB) de iniciar la respuesta inmune : interacción con las células dendríticas. (Thesis). Universidad de Buenos Aires. Retrieved from http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgrauba&cl=CL1&d=HWA_1255 ; http://repositoriouba.sisbi.uba.ar/gsdl/collect/posgrauba/index/assoc/HWA_1255.dir/1255.PDF

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Franco Mahecha, Olga Lucía. “Estudio de la capacidad de antígenos del virus de la diarrea viral bovina (VDVB) de iniciar la respuesta inmune : interacción con las células dendríticas.” 2014. Thesis, Universidad de Buenos Aires. Accessed January 20, 2021. http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgrauba&cl=CL1&d=HWA_1255 ; http://repositoriouba.sisbi.uba.ar/gsdl/collect/posgrauba/index/assoc/HWA_1255.dir/1255.PDF.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Franco Mahecha, Olga Lucía. “Estudio de la capacidad de antígenos del virus de la diarrea viral bovina (VDVB) de iniciar la respuesta inmune : interacción con las células dendríticas.” 2014. Web. 20 Jan 2021.

Vancouver:

Franco Mahecha OL. Estudio de la capacidad de antígenos del virus de la diarrea viral bovina (VDVB) de iniciar la respuesta inmune : interacción con las células dendríticas. [Internet] [Thesis]. Universidad de Buenos Aires; 2014. [cited 2021 Jan 20]. Available from: http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgrauba&cl=CL1&d=HWA_1255 ; http://repositoriouba.sisbi.uba.ar/gsdl/collect/posgrauba/index/assoc/HWA_1255.dir/1255.PDF.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Franco Mahecha OL. Estudio de la capacidad de antígenos del virus de la diarrea viral bovina (VDVB) de iniciar la respuesta inmune : interacción con las células dendríticas. [Thesis]. Universidad de Buenos Aires; 2014. Available from: http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgrauba&cl=CL1&d=HWA_1255 ; http://repositoriouba.sisbi.uba.ar/gsdl/collect/posgrauba/index/assoc/HWA_1255.dir/1255.PDF

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

2. Marie, Emilie. Synthèse d'imidazo (1,2-a) pyridines à activité antivirale à l'encontre des virus de l'hépatite C et de la diarrhée virale bovine : Synthesis of imidazo[1,2-a]pyridines with antiviral activity against hepatisis C and bovine viral diarrhea viruses.

Degree: Docteur es, Sciences de la vie et de la santé, spécialité Chimie thérapeutique, 2012, Université François-Rabelais de Tours

L’hépatite C est une maladie silencieuse, souvent asymptomatique, mais qui entraîne des lésions du foie et peut évoluer vers une cirrhose et, dans certains cas, vers un cancer. Le carcinome hépatocellulaire engendré par l’hépatite C constitue la première cause de transplantation hépatique. Les virus de l’hépatite C (VHC) et de la diarrhée virale bovine (VDVB) sont deux pestivirus possédant un ARN monocaténaire, de la famille des Flaviviridae. Bien qu’ayant des génomes différents, ils présentent une organisation structurelle et des processus de développement de l’enveloppe cellulaire comparables. Le screening de la chimiothèque du laboratoire a permis d’identifier cinq composés chefs de files, actifs à l’encontre du virus de l’hépatite C. Deux de ces composés de la série imidazo[1,2-a]pyridine ont fait l’objet d’un travail de pharmacomodulation dans le cadre des thèses de Jean-Baptiste Véron et Nicolas Henry. La première partie de mon travail de recherche a donc consisté à poursuivre ces travaux de pharmacomodulation afin de tenter d’améliorer l’activité de cette série chimique à l’égard du VHC ainsi que son index thérapeutique. La synthèse convergente de ces molécules a été effectuée grâce à des couplages métallo-catalysés.La seconde partie de mon projet de recherche a porté sur l’étude de la bifonctionnalisation des positions 7 et 8 du noyau imidazo[1,2-a]pyridine. Ces travaux ont permis de développer de nouvelles méthodologies pour introduire une diversité fonctionnelle sur ces positions. Ces molécules ont également été évaluées à l’encontre du VHC et l’une d’entre elle a montré une activité intéressante à l’encontre de ce virus. L’activité à l’encontre du VHC et l’index thérapeutique ont été améliorés pour deux molécules, analogues du BPIP.

Hepatitis C is a silent disease, often asymptomatic, responsible for hepatic lesions which may lead to cirrhosis and in some cases, to cancer. Hepatocellular carcinoma caused by hepatitis C virus is the leading cause of liver transplantation. Bovine viral diarrhoea (BVDV) and hepatitis C (HCV) viruses are two pestiviruses from the Flaviviridae family that have a single-stranded RNA. Despite having different genomes, they present a similar structural organization and processes of development of the cell envelope.The laboratory’s chemical library screening has identified five hits, active against the HCV. Two of these compounds from the imidazo[1,2-a]pyridine serie were pharmacomodulated as part of the Ph.D. thesis of Jean-Baptiste Véron and Nicolas Henry.The first part of my research work was therefore to continue the pharmacomodulation study of these chemical series to improve their activity against HCV and their therapeutic index. To do so, the convergent synthesis of these molecules was performed using metal-catalyzed couplings.The second part of my project has focused on the study of the difunctionalization of positions 7 and 8 of the imidazo[1,2-a]pyridine nucleus. This work helped to develop new methodologies for introducing a functional diversity on these positions.…

Advisors/Committee Members: Gueiffier, Alain (thesis director).

Subjects/Keywords: Hépatite C; VHC; VDVB; Imidazo[1,2-a]pyridine; Pharmacomodulation; Bifonctionnalisation; Couplages métallo-catalysés; Évaluation biologique; Hepatitis C; HCV; BVDV; Imidazo [1,2-a]pyridine; Pharmacomodulation; Bifunctionalization; Metallo-catalyzed cross-coupling; Biological evaluation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Marie, E. (2012). Synthèse d'imidazo (1,2-a) pyridines à activité antivirale à l'encontre des virus de l'hépatite C et de la diarrhée virale bovine : Synthesis of imidazo[1,2-a]pyridines with antiviral activity against hepatisis C and bovine viral diarrhea viruses. (Doctoral Dissertation). Université François-Rabelais de Tours. Retrieved from http://www.theses.fr/2012TOUR3802

Chicago Manual of Style (16th Edition):

Marie, Emilie. “Synthèse d'imidazo (1,2-a) pyridines à activité antivirale à l'encontre des virus de l'hépatite C et de la diarrhée virale bovine : Synthesis of imidazo[1,2-a]pyridines with antiviral activity against hepatisis C and bovine viral diarrhea viruses.” 2012. Doctoral Dissertation, Université François-Rabelais de Tours. Accessed January 20, 2021. http://www.theses.fr/2012TOUR3802.

MLA Handbook (7th Edition):

Marie, Emilie. “Synthèse d'imidazo (1,2-a) pyridines à activité antivirale à l'encontre des virus de l'hépatite C et de la diarrhée virale bovine : Synthesis of imidazo[1,2-a]pyridines with antiviral activity against hepatisis C and bovine viral diarrhea viruses.” 2012. Web. 20 Jan 2021.

Vancouver:

Marie E. Synthèse d'imidazo (1,2-a) pyridines à activité antivirale à l'encontre des virus de l'hépatite C et de la diarrhée virale bovine : Synthesis of imidazo[1,2-a]pyridines with antiviral activity against hepatisis C and bovine viral diarrhea viruses. [Internet] [Doctoral dissertation]. Université François-Rabelais de Tours; 2012. [cited 2021 Jan 20]. Available from: http://www.theses.fr/2012TOUR3802.

Council of Science Editors:

Marie E. Synthèse d'imidazo (1,2-a) pyridines à activité antivirale à l'encontre des virus de l'hépatite C et de la diarrhée virale bovine : Synthesis of imidazo[1,2-a]pyridines with antiviral activity against hepatisis C and bovine viral diarrhea viruses. [Doctoral Dissertation]. Université François-Rabelais de Tours; 2012. Available from: http://www.theses.fr/2012TOUR3802

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