subject:(Ulva fasciata)

1. Daniel Barroso de Alencar. ProspecÃÃo quÃmica da macroalga marinha verde Ulva fasciata Delile.

Degree: Master, 2010, Universidade Federal do Ceará

URL: http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=6565 ;

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As macroalgas marinhas sÃo consideradas fontes promissoras de compostos bioativos para estudos fitoquÃmicos. Tais compostos possuem diferentes propriedades biolÃgicas, funcionando como antibacterianos, antifÃngicos, antivirais, anti-inflamatÃrios,… (more)

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As macroalgas marinhas sÃo consideradas fontes promissoras de compostos bioativos para estudos fitoquÃmicos. Tais compostos possuem diferentes propriedades biolÃgicas, funcionando como antibacterianos, antifÃngicos, antivirais, anti-inflamatÃrios, anti-helmÃnticos, antileishmaniose, antimalÃria, antioxidantes, antitumorais cuja utilizaÃÃo como fÃrmacos tem despertado o interesse de muitos pesquisadores. O objetivo deste trabalho foi realizar a investigaÃÃo fitoquÃmica do extrato etanÃlico da macroalga marinha verde Ulva fasciata. O material foi coletado em abril de 2008, na Praia do Pacheco, no municÃpio de Caucaia-CE. As macroalgas in natura foram desidratadas em estufa com recirculaÃÃo de ar a 40ÂC por 15 horas e, em seguida, trituradas, obtendo-se 500 g, os quais foram submetidos primeiramente a uma extraÃÃo a frio com hexano (UFH), e posteriormente com etanol a 70% (UFE). Para o isolamento e a purificaÃÃo dos constituintes fixos foi empregada uma combinaÃÃo de tÃcnicas cromatogrÃficas clÃssicas em que foram isolados UF-1, UF-2 e UF-3. Na amostra UF-1 foi observada a presenÃa de um constituinte majoritÃrio (86,17%) com tempo de retenÃÃo de 24,940 min, cujo espectro de massas o caracterizou por apresentar o Ãon molecular M+ 284. A sugestÃo fornecida pela anÃlise CG-EM e a comparaÃÃo visual do espectro do composto analisado com os da literatura, bem como pela proposta mecanÃstica de formaÃÃo dos principais fragmentos reforÃaram sua identificaÃÃo como hexadecanoato de etila com um Ãndice de similaridade de 96%. Os compostos UF-2 e UF-3 foram encaminhados para o Centro Nordestino de AplicaÃÃo e Uso da RessonÃncia MagnÃtica Nuclear (CENAUREMN) da Universidade Federal do CearÃ, para a determinaÃÃo sua estrutura quÃmica atravÃs de tÃcnicas espectroscÃpicas de RMN 1H e 13C uni- e bidimensionais (1H, 1H-COSY, gs-HMQC, gs-HMBC, ROESY, NOESY e TOCSY), bem como por espectrometria de massa (EM).

Marine macroalgae have been considered promising sources of bioactive compounds. These compounds exhibit different biological properties, such as antibacterial, antifungal, antiviral, anti-inflammatory, antihelminthic, antileshmaniose, antimalaria, antioxidants, and antitumor. Their use as medicine has spurred the interest of many researches. The objective of this work was to carry out a phytochemistry investigation of the ethanolic extract of green marine macroalga Ulva fasciata. The plant material was collected in April 2008 at Pacheco Beach, Caucaia-CE. Macroalgae in natura were dehydrated in an air-circulation oven at 40ÂC for 15 hours. After the drying process, the material was cut into small pieces. 500 grams were submitted to a cold extraction with hexane (UFH) followed by an extraction with 70% ethanol (UFE). Both isolation and purification from fixed constituents were performed using a combination of classic chromatographic techniques. Three samples were isolated and named UF-1, UF-2 and UF-3. The presence of a major constituent (86.17%) with retention time of 24.940 min was observed in the first sample (UF-1), and its mass…

Advisors/Committee Members: Alexandre Holanda Sampaio, Silvana Saker Sampaio, Francisco Arnaldo Viana.

Subjects/Keywords: RECURSOS PESQUEIROS E ENGENHARIA DE PESCA; Produtos naturais marinhos; Ulva fasciata; hexadecanoato de etila; Marine natural products; Ulva fasciata; Ethyl hexadecanoate; Compostos bioativos; Produtos naturais; ClorÃfitas; Ulva; QuÃmica vegetal

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Alencar, D. B. d. (2010). ProspecÃÃo quÃmica da macroalga marinha verde Ulva fasciata Delile. (Masters Thesis). Universidade Federal do Ceará. Retrieved from http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=6565 ;

Chicago Manual of Style (16^th Edition):

Alencar, Daniel Barroso de. “ProspecÃÃo quÃmica da macroalga marinha verde Ulva fasciata Delile.” 2010. Masters Thesis, Universidade Federal do Ceará. Accessed March 05, 2021. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=6565 ;.

MLA Handbook (7^th Edition):

Alencar, Daniel Barroso de. “ProspecÃÃo quÃmica da macroalga marinha verde Ulva fasciata Delile.” 2010. Web. 05 Mar 2021.

Vancouver:

Alencar DBd. ProspecÃÃo quÃmica da macroalga marinha verde Ulva fasciata Delile. [Internet] [Masters thesis]. Universidade Federal do Ceará 2010. [cited 2021 Mar 05]. Available from: http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=6565 ;.

Council of Science Editors:

Alencar DBd. ProspecÃÃo quÃmica da macroalga marinha verde Ulva fasciata Delile. [Masters Thesis]. Universidade Federal do Ceará 2010. Available from: http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=6565 ;

NSYSU

2. Wu, Tsung-meng. Gene expression in marine macroalga Ulva fasciata Delile against excess copper toxicity.

Degree: PhD, Marine Biology, 2009, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-1228109-120140

► This is the first research by using suppression subtractive hybridization (SSH) to analysis the gene expression in marine macroalga Ulva fasciata Delile against excess copper… (more)

▼ This is the first research by using suppression subtractive hybridization (SSH) to analysis the gene expression in marine macroalga Ulva fasciata Delile against excess copper toxicity, and it gives us a comprehensive understanding of the tolerant mechanism while macroalgae face to the excess copper. Suppression subtractive hybridization was used to identify genes differentially expressed following exposure to 50 Î¼M CuSO4 for 6- 12h in a marine macroalga Ulva fasciata Delile. In this work, 69 genes were identified, of which 55 were up-regulated and 14 were down-regulated. According to the database of Gene Ontology (GO), these genes were classified into 10 categories as follows: 1. Transcription; 2. Translation, ribosomal structure and biogenesis; 3. Posttranslational modification, protein turnover, chaperones; 4. Photosynthesis; 5. Cell redox homeostasis; 6. Stress; 7. Metabolism; 8. Energy production and conversion; 9. Transport; 10. Function unknown. According to the results, we suggest that the responses of U. fasciata against excess copper toxicity are mainly through increase of the energy production for providing sufficient energy to many metabolic pathways, and control of the Fe homeostasis and redox form of thiol groups for maintaining the cellular redox homeostasis, moreover, expression of photosynthetic genes for letting the photosynthesis work. In addition, to scavenge the ROS is by expression of stress-related genes, meanwhile, the proteins, DNA and lipids damaged by ROS (reactive oxygen species) and copper are repaired by expression of the other categorical genes. Over and above, the genes expressing in the metabolism category might maintain the amino acids homeostasis and increase the purine content, and subsequently increase the tolerant capacity of U. fasciata against excess copper toxicity. In addition, the concentrations of antioxidants and the activities and gene expression of antioxidant enzymes were determined in Ulva fasciata Delile by a 4-day exposure to 0, 5, 10, 20 and 50 Î¼M CuSO4. These results demonstrate that the maintenance of antioxidant homeostasis and the induction of activities of antioxidant enzymes via enhanced gene expression are used by U. fasciata to cope with the Cu-induced oxidative stress, but the defense capacity cannot sufficiently alleviate oxidative damage occurring under the condition of higher Cu concentrations. Moreover, according to the results from the expression of genes involved in the control of redox homeostasis and antioxidant defense was studied in macroalga Ulva fasciata Delile in response to CuSO4 (5 and 50 Î¼M) and ROS (H2O2 and O2Ë-), we suggest that ROS involved in up-regulation of antioxidant defense-related genes and the expression of genes of antioxidant defense enzymes and UfMsrA (methionine sulfoxide reductase A) are associated with long-term adaptation of U. fasciata to Cu excess and transcription of redox- related genes and UfGr (glutathione reductase) is up-regulated for short-term acclimation. Promoters play a key role in regulating gene… Advisors/Committee Members: Hao-Jen Huang (chair), Zin-Huang Liu (chair), Kuo-Chen Yeh (chair), Ming-Tsair Chan (chair), Yi-Ting Hsu (chair), Tse-Min Lee (committee member), Ching-Huei Kao (chair).

Subjects/Keywords: Ulva fasciata; copper; heavy metal; oxidative stress; redox homeostasis; gene expression; suppression subtractive hybridization

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wu, T. (2009). Gene expression in marine macroalga Ulva fasciata Delile against excess copper toxicity. (Doctoral Dissertation). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-1228109-120140

Chicago Manual of Style (16^th Edition):

Wu, Tsung-meng. “Gene expression in marine macroalga Ulva fasciata Delile against excess copper toxicity.” 2009. Doctoral Dissertation, NSYSU. Accessed March 05, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-1228109-120140.

MLA Handbook (7^th Edition):

Wu, Tsung-meng. “Gene expression in marine macroalga Ulva fasciata Delile against excess copper toxicity.” 2009. Web. 05 Mar 2021.

Vancouver:

Wu T. Gene expression in marine macroalga Ulva fasciata Delile against excess copper toxicity. [Internet] [Doctoral dissertation]. NSYSU; 2009. [cited 2021 Mar 05]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-1228109-120140.

Council of Science Editors:

Wu T. Gene expression in marine macroalga Ulva fasciata Delile against excess copper toxicity. [Doctoral Dissertation]. NSYSU; 2009. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-1228109-120140

NSYSU

3. Huang, Ya-Ling. Effects of copper on phosphorus utilization in Ulva fasciata Delile (Ulvales, Chlorophyta).

Degree: Master, Marine Biology, 2002, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0730102-214743

► The effects of copper on growth and phosphorus (P) utilization were investigated in the marine chlorophyte Ulva fasciata Delile. Both the daily specific growth rate… (more)

Subjects/Keywords: phosphorus; Ulva fasciata; copper; Pi uptake

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Huang, Y. (2002). Effects of copper on phosphorus utilization in Ulva fasciata Delile (Ulvales, Chlorophyta). (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0730102-214743

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Huang, Ya-Ling. “Effects of copper on phosphorus utilization in Ulva fasciata Delile (Ulvales, Chlorophyta).” 2002. Thesis, NSYSU. Accessed March 05, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0730102-214743.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Huang, Ya-Ling. “Effects of copper on phosphorus utilization in Ulva fasciata Delile (Ulvales, Chlorophyta).” 2002. Web. 05 Mar 2021.

Vancouver:

Huang Y. Effects of copper on phosphorus utilization in Ulva fasciata Delile (Ulvales, Chlorophyta). [Internet] [Thesis]. NSYSU; 2002. [cited 2021 Mar 05]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0730102-214743.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Huang Y. Effects of copper on phosphorus utilization in Ulva fasciata Delile (Ulvales, Chlorophyta). [Thesis]. NSYSU; 2002. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0730102-214743

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Technical University of Lisbon

4. Raposo, Diogo Manuel Trindade. Avaliação do desempenho do camarão Litopenaeus vannamei e das macroalgas Gracilaria birdiae e Ulva fasciata num sistema de cultivo multitrófico integrado.

Degree: 2013, Technical University of Lisbon

URL: https://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/6168

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Mestrado em Engenharia Zootécnica - Produção Animal - Instituto Superior de Agronomia / Faculdade de Medicina Veterinária

The aquaculture is growing fast, responding to the… (more)

Subjects/Keywords: integrated multitrophic aquaculture; co-culture; Litopenaeus vannamei; Gracilaria birdiae; Ulva fasciata; bioremediation

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APA (6^th Edition):

Raposo, D. M. T. (2013). Avaliação do desempenho do camarão Litopenaeus vannamei e das macroalgas Gracilaria birdiae e Ulva fasciata num sistema de cultivo multitrófico integrado. (Thesis). Technical University of Lisbon. Retrieved from https://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/6168

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Raposo, Diogo Manuel Trindade. “Avaliação do desempenho do camarão Litopenaeus vannamei e das macroalgas Gracilaria birdiae e Ulva fasciata num sistema de cultivo multitrófico integrado.” 2013. Thesis, Technical University of Lisbon. Accessed March 05, 2021. https://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/6168.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Raposo, Diogo Manuel Trindade. “Avaliação do desempenho do camarão Litopenaeus vannamei e das macroalgas Gracilaria birdiae e Ulva fasciata num sistema de cultivo multitrófico integrado.” 2013. Web. 05 Mar 2021.

Vancouver:

Raposo DMT. Avaliação do desempenho do camarão Litopenaeus vannamei e das macroalgas Gracilaria birdiae e Ulva fasciata num sistema de cultivo multitrófico integrado. [Internet] [Thesis]. Technical University of Lisbon; 2013. [cited 2021 Mar 05]. Available from: https://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/6168.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Raposo DMT. Avaliação do desempenho do camarão Litopenaeus vannamei e das macroalgas Gracilaria birdiae e Ulva fasciata num sistema de cultivo multitrófico integrado. [Thesis]. Technical University of Lisbon; 2013. Available from: https://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/6168

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

NSYSU

5. Lee, Ju-Chien. Ulva fasciata protein disulfide isomerase and thioredoxin expression in response to hypersaline stress.

Degree: PhD, Biological Sciences, 2007, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0906107-172450

► This research has investigated the gene expression of protein disulfide isomerase (PDI; EC 5.3.4.1) and thioredoxin (Trx) in the marine macroalga Ulva fasciata Delile in… (more)

Subjects/Keywords: Salinity stress; Oxidative stress; PDI; Polyamine; Ulva fasciata

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lee, J. (2007). Ulva fasciata protein disulfide isomerase and thioredoxin expression in response to hypersaline stress. (Doctoral Dissertation). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0906107-172450

Chicago Manual of Style (16^th Edition):

Lee, Ju-Chien. “Ulva fasciata protein disulfide isomerase and thioredoxin expression in response to hypersaline stress.” 2007. Doctoral Dissertation, NSYSU. Accessed March 05, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0906107-172450.

MLA Handbook (7^th Edition):

Lee, Ju-Chien. “Ulva fasciata protein disulfide isomerase and thioredoxin expression in response to hypersaline stress.” 2007. Web. 05 Mar 2021.

Vancouver:

Lee J. Ulva fasciata protein disulfide isomerase and thioredoxin expression in response to hypersaline stress. [Internet] [Doctoral dissertation]. NSYSU; 2007. [cited 2021 Mar 05]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0906107-172450.

Council of Science Editors:

Lee J. Ulva fasciata protein disulfide isomerase and thioredoxin expression in response to hypersaline stress. [Doctoral Dissertation]. NSYSU; 2007. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0906107-172450

NSYSU

6. Hsu, Yuan-ting. Signal derived from photosynthic electron transport regulates the expression of methionine sulfoxide reductase (Msr) gene in the green macroalga Ulva fasciata Delile.

Degree: PhD, Marine Biology, 2008, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-1120108-183121

► This study has investigated the involvement of photosynthetic electron transport chain on the regulation of gene expression of methionine sulfoxide reductase (UfMSR) in the marine… (more)

▼ This study has investigated the involvement of photosynthetic electron transport chain on the regulation of gene expression of methionine sulfoxide reductase (UfMSR) in the marine macroalga Ulva fasciata Delile.UfMSRA is from copper stress and UfMSRB ir from hypersalinity stress. UfMSRA is similar to Arabidopsis AtMSRA4 and UfMSRB is similar to AtMSRB1. UfMSRA is specific to the MetSO S-enantiomer and UfMSRB catalytically reduces the MetSO R-enantiomer. Both enzymes are required, since in the cell oxidation of Met residues at the sulfur atom results in a racemic mixture of the two stereoisomers. UfMSRA and UfMSRB transcripts were increased by white light, blue light and red light with the maximum at 1 h following a decline, but kept constant in the dark. The magnitude of UfMSRA and UfMSRB transcript increase showed a positive linear correlation to increasing light intensity from 0-1200 u moleÂ·m-2Â·s-1. The treatment with linear electron transport chain inhibitors, hydroxylamine, 3-(3,4-dichlorophenyl) -1,1-dimethylurea (DCMU), 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone (DBMIB) and stigmatellin, effectively inhibited PS II activity under 300 u moleÂ·m-2Â·s-1 irradiance. DBMIB and stigmatellin can increase UfMSRA transcript that was reversed by 2,6-dichlorophenolindophenol (DCPIP), a PS I electron donor. It indicates that the block of electron transport of the downstream of cytochrome b6f indeuces UfMSRA gene expression. Hydroxylamine, DCMU and DBMIB decreased UfMSRB transcript that was not reversed by DCPIP while stigmatellin increased UfMSRB mRNA level, reflecting a role of reduced state with Qo site located at cytochrome b6f on the induction of UfMSRB gene expression. The cyclic electron transport chain inhibitors, antimycin A that inhibited photosynthetic electron transport, can inhibit the increase of UfMSRA and UfMSRB transcripts by irradiance. UfMSRA and UfMSRB gene expression were both modulated by cyclic electron transport chain and linear electron transport chain. These results reveal that photosynthetic electron transport chain modulates UfMSRA and UfMSRB gene expression by change its redox state. Advisors/Committee Members: Zin-Huang Liu (chair), Tse-Min Lee (committee member), Hsien-Jung Chen (chair).

Subjects/Keywords: DCMU; Ulva fasciata; methionine sulfoxide reductase; electron transport chain; Antimycin A; hydroxylamine; Light; stigmatellin; SHAM; DCPIP; DBMIB

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hsu, Y. (2008). Signal derived from photosynthic electron transport regulates the expression of methionine sulfoxide reductase (Msr) gene in the green macroalga Ulva fasciata Delile. (Doctoral Dissertation). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-1120108-183121

Chicago Manual of Style (16^th Edition):

Hsu, Yuan-ting. “Signal derived from photosynthic electron transport regulates the expression of methionine sulfoxide reductase (Msr) gene in the green macroalga Ulva fasciata Delile.” 2008. Doctoral Dissertation, NSYSU. Accessed March 05, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-1120108-183121.

MLA Handbook (7^th Edition):

Hsu, Yuan-ting. “Signal derived from photosynthic electron transport regulates the expression of methionine sulfoxide reductase (Msr) gene in the green macroalga Ulva fasciata Delile.” 2008. Web. 05 Mar 2021.

Vancouver:

Hsu Y. Signal derived from photosynthic electron transport regulates the expression of methionine sulfoxide reductase (Msr) gene in the green macroalga Ulva fasciata Delile. [Internet] [Doctoral dissertation]. NSYSU; 2008. [cited 2021 Mar 05]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-1120108-183121.

Council of Science Editors:

Hsu Y. Signal derived from photosynthic electron transport regulates the expression of methionine sulfoxide reductase (Msr) gene in the green macroalga Ulva fasciata Delile. [Doctoral Dissertation]. NSYSU; 2008. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-1120108-183121

NSYSU

7. Sung, Ming-Hsuan. The protease genes expression in Ulva fasciata (Ulvales, Chlorophyta) in relation to hypersalinity-induced oxidative stress and protein oxidation.

Degree: Master, Marine Biology, 2006, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0718106-000701

► This study has investigated the gene expression of ubiquitinã20S proteasome beta subunit type 1 (20sÎ²1)ãubiquitin-conjugating enzyme e2 (ucee2)ãATP-dependent caseinolytic protease regulatory subunit (clpC) in the… (more)

▼ This study has investigated the gene expression of ubiquitinã20S proteasome beta subunit type 1 (20sÎ²1)ãubiquitin-conjugating enzyme e2 (ucee2)ãATP-dependent caseinolytic protease regulatory subunit (clpC) in the marine macroalga Ulva fasciata Delile in relation to the hypersalinity-induced oxidative stress and protein oxidation. During the early stage (0-1 h), the water contents and TTC (2,3,5-tripheny tetrazolium chloride) reduction ability maintained unchanged but recovery ability and photosynthetic ability (PS II activity as indicated by Fv/Fm) were decreased along with accumulated H2O2, suggesting the occurrence of oxidative stress. Only ubiquitin expressed at this stage. During 1-3 h, water lost (approximately 33% of the control) with a further decrease in recovery ability, TTC reduction abilityãPS II activity but more H2O2 accumulation and protein carbonyl compound. The transcripts of 20sÎ²1 and clpC and caseinolytic protease activity increased at this stage with the maximum of clpC at hour 3. In the 6-48 h, water lost seriously with high accumulated free amino acid at 6-12 h but low recovery ability. The transcript amounts of ubiquitinã20sÎ²1 and ucee2 increased marked during this stage, in which these might be related to programmed cell death caused by long-term exposure to hypersalinity. Reactive oxygen species (ROS) scavengers inhibited H2O2 accumulation, caseinolytic proteolytic activity increase, carbonyl compound formation and gene expression of ubiquitinã20sÎ²1ãucee2ãclpC, indicating a role of ROS in the regulation of protease genes. A role of polyamines in the regulation of protease gene expression was tested. Spermidine and spermine inhibited the gene expression of ubiquitinã20sÎ²1 and ucee2, the oxidation of proteins (carbonyl groups) and the induction of caseinolytic protease activity in 90‰-treated thalli, whereas putrescine inhibited clpC expression, the oxidation of proteins and caseinolytic protease activity but enhanced the gene expression of ubiquitinã20sÎ²1 and ucee2. In conclusion, the results of the present investigation show that the degradation of oxidatively damaged proteins under hypersalinity conditions by increased caseinolytic protease activity is driven by the up-regulation of clpC gene expression via ROS and polyamines. It seems likely that the induction of ubiquitinã20sÎ²1 and ucee2 gene expression might be associated with the hypersalinity-mediated programmed cell death. Advisors/Committee Members: Ming-Tsair Chan (chair), Tse-Min Lee (committee member), Yaw-Huei Lin (chair).

Subjects/Keywords: Protease; Salinity stress; Oxidative stress; Ulva fasciata

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sung, M. (2006). The protease genes expression in Ulva fasciata (Ulvales, Chlorophyta) in relation to hypersalinity-induced oxidative stress and protein oxidation. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0718106-000701

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Sung, Ming-Hsuan. “The protease genes expression in Ulva fasciata (Ulvales, Chlorophyta) in relation to hypersalinity-induced oxidative stress and protein oxidation.” 2006. Thesis, NSYSU. Accessed March 05, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0718106-000701.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Sung, Ming-Hsuan. “The protease genes expression in Ulva fasciata (Ulvales, Chlorophyta) in relation to hypersalinity-induced oxidative stress and protein oxidation.” 2006. Web. 05 Mar 2021.

Vancouver:

Sung M. The protease genes expression in Ulva fasciata (Ulvales, Chlorophyta) in relation to hypersalinity-induced oxidative stress and protein oxidation. [Internet] [Thesis]. NSYSU; 2006. [cited 2021 Mar 05]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0718106-000701.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Sung M. The protease genes expression in Ulva fasciata (Ulvales, Chlorophyta) in relation to hypersalinity-induced oxidative stress and protein oxidation. [Thesis]. NSYSU; 2006. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0718106-000701

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

8. Abirami, R G. Nutritional and safety evaluation of underexploited seaweeds and nutraceutical potentials of ulva fasciata;.

Degree: Food Science and Nutrition, 2012, Avinashilingam Deemed University For Women

URL: http://shodhganga.inflibnet.ac.in/handle/10603/13460

None

Bibliography p.196-215

Advisors/Committee Members: Kowsalya S.

Subjects/Keywords: Food Science and Nutrition; Ulva fasciata; Underexploited seaweeds

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APA (6^th Edition):

Abirami, R. G. (2012). Nutritional and safety evaluation of underexploited seaweeds and nutraceutical potentials of ulva fasciata;. (Thesis). Avinashilingam Deemed University For Women. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/13460

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Abirami, R G. “Nutritional and safety evaluation of underexploited seaweeds and nutraceutical potentials of ulva fasciata;.” 2012. Thesis, Avinashilingam Deemed University For Women. Accessed March 05, 2021. http://shodhganga.inflibnet.ac.in/handle/10603/13460.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Abirami, R G. “Nutritional and safety evaluation of underexploited seaweeds and nutraceutical potentials of ulva fasciata;.” 2012. Web. 05 Mar 2021.

Vancouver:

Abirami RG. Nutritional and safety evaluation of underexploited seaweeds and nutraceutical potentials of ulva fasciata;. [Internet] [Thesis]. Avinashilingam Deemed University For Women; 2012. [cited 2021 Mar 05]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/13460.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Abirami RG. Nutritional and safety evaluation of underexploited seaweeds and nutraceutical potentials of ulva fasciata;. [Thesis]. Avinashilingam Deemed University For Women; 2012. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/13460

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

9. Sudhir Kumar. Isolation and studies of marine algae Lectins;.

Degree: Biotechnology, 2011, Goa University

URL: http://shodhganga.inflibnet.ac.in/handle/10603/7543

None

References p. 191-230, Appendix included

Advisors/Committee Members: – .

Subjects/Keywords: Biotechnology; Marine algae; Lectins; Ulva fasciata; Caulerpa sertularioides

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APA (6^th Edition):

Kumar, S. (2011). Isolation and studies of marine algae Lectins;. (Thesis). Goa University. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/7543

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kumar, Sudhir. “Isolation and studies of marine algae Lectins;.” 2011. Thesis, Goa University. Accessed March 05, 2021. http://shodhganga.inflibnet.ac.in/handle/10603/7543.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kumar, Sudhir. “Isolation and studies of marine algae Lectins;.” 2011. Web. 05 Mar 2021.

Vancouver:

Kumar S. Isolation and studies of marine algae Lectins;. [Internet] [Thesis]. Goa University; 2011. [cited 2021 Mar 05]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/7543.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kumar S. Isolation and studies of marine algae Lectins;. [Thesis]. Goa University; 2011. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/7543

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

NSYSU

10. Lin , Kai-Chih. Studies on the Enhancement of Salt Tolerance of Rice (Oryza sativa L.) Seedlings by Overexpression of Glutathione Reductase from Marine Macroalga Ulva fasciata Delile.

Degree: Master, Department of Oceanography, 2014, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0115114-151453

► This study was to examine whether the Agrobacterium-mediated overexpression of a marine macroalgal glutathione reductase (UfGR; NCBI number DQ286546.1) of Ulva fasciata Delile in Japonica… (more)

▼ This study was to examine whether the Agrobacterium-mediated overexpression of a marine macroalgal glutathione reductase (UfGR; NCBI number DQ286546.1) of Ulva fasciata Delile in Japonica rice (Oryza sativa L. cv. Tainung 67, TNG67) improves the tolerance of rice to salt stress. Using the transformation of ubiquitin:: UfGR + 35S::GFP, the hydroponically grown seedling (4-leaf age) of transgenic line with homozygous gene expression (T2) were used to determine the GR activity and their responses to salt challenge (150 mM NaCl). Transgenic lines, OE-UfGR-1 and OE-UfGR-3 that GR specific activity is higher than wild type showed higher survival ratio after recovery to normal condition without NaCl. Under 150 mM NaCl conditions,the decrease in effective PSII activity (Fv/Fm), maximum PSII activity (Fv'/Fm') and the contents of chlorphyll a, chlorophyll b, and carotenoids in the transgenic lines is lower than those in wild type. Transgenic lines showed a lower H2O2 and malondialdehyde (MDA, lipid peroxidation indiicator) contents following NaCl treatment, but the increae in proline contents is higher in transgenic lines as compared to the wild type after NaCl treatment. The GR specific activity of transgenic lines could be induced under NaCl treatment and the induction is higher in transgenic lines, while the specific activity of ascorbate peroxidase (APX) and catalase (CAT) showed a similar trend. Glutathione (GSH) content, GSH/oxidized glutathione (GSSG) ratio, ascorbate (AsA) content, and AsA/dehydroascorbate (DHA) ratio were highly induced by NaCl treatment, particularly in the transgenic lines. But, the increae in Na+ content as well as the decrease in K+ contents and K+/Na+ ratio by NaCl treatment were same between wild type and transgenic lines. It could be concluded that overexpression of UfGR effectively enhances the tolerance of rice to slat stress via the enhancement of ascorbateâglutathione cycle for the reduction of oxidative damage under salt stress, but not the regulation of K+ and Na+ content. Advisors/Committee Members: none (chair), none (chair), none (committee member).

Subjects/Keywords: maximum PSII activity (Fv'/Fm'); transformation; effective PSII activity (Fv/Fm); Na+; K+; proline; catalase; ascorbate; ascorbate peroxidase; salt stress; glutathione; Ulva fasciata; glutathione reductase; rice

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lin , K. (2014). Studies on the Enhancement of Salt Tolerance of Rice (Oryza sativa L.) Seedlings by Overexpression of Glutathione Reductase from Marine Macroalga Ulva fasciata Delile. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0115114-151453

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lin , Kai-Chih. “Studies on the Enhancement of Salt Tolerance of Rice (Oryza sativa L.) Seedlings by Overexpression of Glutathione Reductase from Marine Macroalga Ulva fasciata Delile.” 2014. Thesis, NSYSU. Accessed March 05, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0115114-151453.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lin , Kai-Chih. “Studies on the Enhancement of Salt Tolerance of Rice (Oryza sativa L.) Seedlings by Overexpression of Glutathione Reductase from Marine Macroalga Ulva fasciata Delile.” 2014. Web. 05 Mar 2021.

Vancouver:

Lin K. Studies on the Enhancement of Salt Tolerance of Rice (Oryza sativa L.) Seedlings by Overexpression of Glutathione Reductase from Marine Macroalga Ulva fasciata Delile. [Internet] [Thesis]. NSYSU; 2014. [cited 2021 Mar 05]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0115114-151453.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lin K. Studies on the Enhancement of Salt Tolerance of Rice (Oryza sativa L.) Seedlings by Overexpression of Glutathione Reductase from Marine Macroalga Ulva fasciata Delile. [Thesis]. NSYSU; 2014. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0115114-151453

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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