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You searched for subject:(Tubulin folding pathway). Showing records 1 – 2 of 2 total matches.

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1. Hage-Sleiman, Rouba. Impact of tululin binding cofactor C (TBCC) on microtubule mass and dynamics, cell cycle, tumor growth and response to chemotherapy in breast cancer : Effets de la protéine tubulin binding cofactor C (TBCC) sur la masse et la dynamique microtubulaire, le cycle cellulaire, la croissance tumorale et la réponse à la chimiothérapie dans le cancer du sein.

Degree: Docteur es, Cancérologie, 2010, Université Claude Bernard – Lyon I

La mise en conformation de l’α et β tubulines en hétérodimeres polymérisables nécessite l’intervention de cinq protéines « Tubulin Binding Cofactors » (TBCA a TBCE) dont TBCC qui joue un rôle indispensable. Dans des cellules humaines d’adénocarcinome mammaire, nous avons modifié le niveau d’expression de TBCC et nous avons montre que ceci avait un impact sur le contenu des fractions de tubuline, la dynamique des microtubules ainsi que sur le phénotype et chimiosensibilité des cellules. La distribution en cycle cellulaire et les durées de la mitose et de la phase S ont été altérées. La modification de TBCC avait un faible effet sur la vitesse de prolifération in vitro par contre les cellules présentaient des différences significatives de croissance tumorale in vivo. Les réponses aux agents antimicrotubulaires et à la gemcitabine ont montrées une chimiosensibilité dépendante de la distribution en cycle cellulaire. Tous ces résultats montrent l’importance de la régulation du contenu en tubulines et l’impact de ceci sur le comportement de la cellule en général et vis-à-vis des traitements

The proper folding pathway of α and β-tubulin into the α/β-tubulin heterodimers involve five Tubulin Binding Cofactors (TBCA to TBCE). TBCC plays a crucial role in the formation of polymerization-competent the α/β-tubulin heterodimers. To evaluate the impact of microtubule mass and dynamics on the phenotype and chemosensitivity of breast cancer cells, we targeted TBCC in human breast adenocarcinoma and developed variants of breast cancer cells with modified content of TBCC. We have shown that the modifications in TBCC expression level influenced tubulin fraction distribution and microtubule dynamics. Cell cycle distribution and the durations of mitosis and S-phase were altered. The proliferation rate in vitro was slightly modified whereas in vivo the TBCC variants presented major differences in tumor growth capacity. Chemosensitivity to antimicrotubule agents (paclitaxel and vinorelbine) as well as to gemcitabine was observed to be dependent on the cell cycle distribution of the TBCC variants. These results underline the essential role of fine tuned regulation of tubulin content in tumor cells and the major impact of dysregulation of tubulin dimer content on tumor cell phenotype, cell cycle progression and response to chemotherapy. A better understanding of how the microtubule cytoskeleton is dysregulated in cancer cells would greatly contribute to a better understanding of tumor cell biology and characterization of resistant phenotypes

Advisors/Committee Members: Dumontet, Charles (thesis director).

Subjects/Keywords: Tubulin Binding Cofactors C (TBCC); Voie de repliement de la tubuline; Microtubule; Dynamique des microtubules; Cycle cellulaire; Cancer du sein; Agents antimicrotubulaires; Tubulin Binding Cofactors C (TBCC); Tubulin folding pathway; Microtubule; Microtubule dynamics; Cell cycle; Breast cancer; Antimicrotubule agents; 616.994

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Hage-Sleiman, R. (2010). Impact of tululin binding cofactor C (TBCC) on microtubule mass and dynamics, cell cycle, tumor growth and response to chemotherapy in breast cancer : Effets de la protéine tubulin binding cofactor C (TBCC) sur la masse et la dynamique microtubulaire, le cycle cellulaire, la croissance tumorale et la réponse à la chimiothérapie dans le cancer du sein. (Doctoral Dissertation). Université Claude Bernard – Lyon I. Retrieved from http://www.theses.fr/2010LYO10085

Chicago Manual of Style (16th Edition):

Hage-Sleiman, Rouba. “Impact of tululin binding cofactor C (TBCC) on microtubule mass and dynamics, cell cycle, tumor growth and response to chemotherapy in breast cancer : Effets de la protéine tubulin binding cofactor C (TBCC) sur la masse et la dynamique microtubulaire, le cycle cellulaire, la croissance tumorale et la réponse à la chimiothérapie dans le cancer du sein.” 2010. Doctoral Dissertation, Université Claude Bernard – Lyon I. Accessed November 12, 2019. http://www.theses.fr/2010LYO10085.

MLA Handbook (7th Edition):

Hage-Sleiman, Rouba. “Impact of tululin binding cofactor C (TBCC) on microtubule mass and dynamics, cell cycle, tumor growth and response to chemotherapy in breast cancer : Effets de la protéine tubulin binding cofactor C (TBCC) sur la masse et la dynamique microtubulaire, le cycle cellulaire, la croissance tumorale et la réponse à la chimiothérapie dans le cancer du sein.” 2010. Web. 12 Nov 2019.

Vancouver:

Hage-Sleiman R. Impact of tululin binding cofactor C (TBCC) on microtubule mass and dynamics, cell cycle, tumor growth and response to chemotherapy in breast cancer : Effets de la protéine tubulin binding cofactor C (TBCC) sur la masse et la dynamique microtubulaire, le cycle cellulaire, la croissance tumorale et la réponse à la chimiothérapie dans le cancer du sein. [Internet] [Doctoral dissertation]. Université Claude Bernard – Lyon I; 2010. [cited 2019 Nov 12]. Available from: http://www.theses.fr/2010LYO10085.

Council of Science Editors:

Hage-Sleiman R. Impact of tululin binding cofactor C (TBCC) on microtubule mass and dynamics, cell cycle, tumor growth and response to chemotherapy in breast cancer : Effets de la protéine tubulin binding cofactor C (TBCC) sur la masse et la dynamique microtubulaire, le cycle cellulaire, la croissance tumorale et la réponse à la chimiothérapie dans le cancer du sein. [Doctoral Dissertation]. Université Claude Bernard – Lyon I; 2010. Available from: http://www.theses.fr/2010LYO10085


Technical University of Lisbon

2. Cardoso, Rita Isabel de Amorim. Besnoitia besnoiti and Toxoplasma gondii invasion : the role of the parasite's tubulin folding pathway and manipulation of host cell organization.

Degree: 2014, Technical University of Lisbon

Tese de Doutoramento em Ciências Veterinárias- Especialidade de Ciências Biológicas e Biomédicas

Besnoitia besnoiti and Toxoplasma gondii, the etiological agents of besnoitiosis and toxoplasmosis, respectively, are two apicomplexan parasites unable to replicate outside the host cell. In order to survive inside the host cell, these parasites have developed strategies to subvert the cytoskeleton and the endomembrane system of the host cell. In this work, the strategies used by B. besnoiti and T. gondii to manipulate the cytoskeleton, remodeling microtubules (MTs), and interfering with the centrosome and Golgi apparatus of the host cell are studied. We observed that the parasitophorous vacuole (PV) of both parasites is surrounded by host MTs, but only T. gondii recruits the host cell centrosome towards the PV. However, the host Golgi apparatus is recruited to the PV by both parasites but its organization is affected in different ways. The differences found between these two parasites are most likely a result of two distinct evolutionary mechanisms and might reflect the different tissue tropism and pathogeny. Since not only the host cell cytoskeleton, but also the cytoskeleton of the parasite, participate in the establishment of infection, this work also addresses the role of the parasite cytoskeleton during entry and development inside the host cell. For this we propose that components of the tubulin folding pathway are good candidates to regulate cytoskeleton dynamics and reorganization during host invasion. Thus, we started the characterization of the gene structure and expression patterns of the components of tubulin folding pathway (CCTα, TBCB, TBCE and α-tubulin). These studies suggest that these proteins have an important role in parasite replication. Overall, our results contribute to the present knowledge of the mechanisms underlying host cell invasion by these parasites, which might be important for the definition of future therapeutic strategies.

RESUMO - Estudo da invasão por Besnoitia besnoiti e Toxoplasma gondii: papel de componentes da via de aquisição da estrutura tridimensional nativa da tubulina do parasita e manipulação da organização da célula hospedeira - Besnoitia besnoiti e Toxoplasma gondii, agentes etiológicos da besnoitiose e toxoplasmose, respetivamente, são dois parasitas do filo Apicomplexa incapazes de se replicarem fora da célula hospedeira. De forma a sobreviver dentro da célula hospedeira estes parasitas desenvolveram estratégias para subverter o citoesqueleto e sistema endomembranar da célula. Neste trabalho são estudadas as estratégias utilizadas por B. besnoiti e T. gondii para manipular o citoesqueleto, remodelar microtúbulos (MTs), e interferir com o centrossoma e o aparelho de Golgi da célula hospedeira. Observámos que o vacúolo parasitóforo (VP) dos dois parasitas está rodeado de MTs da célula hospedeira, mas apenas T.gondii desloca o centrossoma para o VP. No entanto, o aparelho de Golgi é recrutado para próximo do VP por ambos os parasitas, sendo a sua organização afetada…

Advisors/Committee Members: Leitão, José Alexandre da Costa Perdigão e Cameira, Soares, Maria Helena Antunes.

Subjects/Keywords: Besnoitia besnoiti; Toxoplasma gondii; Microtubule cytoskeleleton; Golgi apparatus; Centrosome; Tubulin folding pathway; Citoesqueleto de microtúbulos; Aparelho de Golgi; Centrossoma; Via de aquisição da estrutura tridimensional nativa da tubulina

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Cardoso, R. I. d. A. (2014). Besnoitia besnoiti and Toxoplasma gondii invasion : the role of the parasite's tubulin folding pathway and manipulation of host cell organization. (Thesis). Technical University of Lisbon. Retrieved from http://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/7753

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Cardoso, Rita Isabel de Amorim. “Besnoitia besnoiti and Toxoplasma gondii invasion : the role of the parasite's tubulin folding pathway and manipulation of host cell organization.” 2014. Thesis, Technical University of Lisbon. Accessed November 12, 2019. http://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/7753.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Cardoso, Rita Isabel de Amorim. “Besnoitia besnoiti and Toxoplasma gondii invasion : the role of the parasite's tubulin folding pathway and manipulation of host cell organization.” 2014. Web. 12 Nov 2019.

Vancouver:

Cardoso RIdA. Besnoitia besnoiti and Toxoplasma gondii invasion : the role of the parasite's tubulin folding pathway and manipulation of host cell organization. [Internet] [Thesis]. Technical University of Lisbon; 2014. [cited 2019 Nov 12]. Available from: http://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/7753.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Cardoso RIdA. Besnoitia besnoiti and Toxoplasma gondii invasion : the role of the parasite's tubulin folding pathway and manipulation of host cell organization. [Thesis]. Technical University of Lisbon; 2014. Available from: http://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/7753

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

.