subject:(Trypanosoma theileri Trypanosoma cervi)

Texas A&M University

1. Fisher, Amanda. Morphological and Genetic Comparisons between Babesia bovis and Trypanosoma spp. Found in Cattle and White-tailed Deer.

Degree: MS, Laboratory Animal Medicine, 2012, Texas A&M University

URL: http://hdl.handle.net/1969.1/ETD-TAMU-2012-08-11676

► Babesia bovis has been an important disease agent in the U.S. cattle industry for over a century. Recently, B. bovis-like parasites have been identified in… (more)

▼ Babesia bovis has been an important disease agent in the U.S. cattle industry for over a century. Recently, B. bovis-like parasites have been identified in white-tailed deer (WTD; Odocoileus virginianus) in Texas. If the parasites found in the WTD are B. bovis that are able to infect cattle, the disease could re-emerge. Susceptible adult cattle often die from this disease, which would result in severe production losses, as well as a decrease in carcass weights of disease survivors. The B. bovis-like parasite found in WTD was compared to B. bovis from cattle, by ribosomal DNA sequence analysis. Babesia isolated from WTD were found to have 99% identity to B. bovis from GenBank cattle sequences. No cattle samples in this study were found to be positive for B. bovis. On culture of WTD samples, a Babesia parasite could not be visualized based on common morphological features. Trypanosoma cervi has been studied for decades, but all the previous research identified this parasite solely by morphology. Trypanosoma species obtained from different host species was compared by ribosomal DNA sequence analyses. In this study, the Trypanosoma cultured from WTD had the morphological appearance of T. cervi. On sequence analysis, the cattle sequences aligned together with cattle isolates and the WTD sequences aligned closely with elk (Cervus canadensis) sequences, indicating that wild ungulates (WTD and elk) and cattle most likely have separate trypanosome species. On distribution analysis there was a trend in three South Texas counties, where the county with the highest occurrence of Trypanosoma had the lowest occurrence of Babesia; and vice versa. It is possible that Trypanosoma and Babesia blood parasites compete within the mammalian host, but the chi-squared test did not show a significant association between the two parasites in the different counties. On seasonal analysis, the correlation between positive samples and season could not be statistically confirmed, but it appears that Babesia infected animals are found in lowest numbers during hot, dry seasons. It also appears that there is another vector for Trypanosoma in South Texas besides the ked (Lipoptena mazamae) and tabanid fly (Tabanus spp.). Advisors/Committee Members: Davis, Donald (advisor), Holman, Patricia J. (advisor), Gresham, Vincent C. (committee member).

Subjects/Keywords: parasite; Trypanosome; Trypanosoma theileri, Trypanosoma cervi; Babesia; cattle; white-tailed deer

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APA (6^th Edition):

Fisher, A. (2012). Morphological and Genetic Comparisons between Babesia bovis and Trypanosoma spp. Found in Cattle and White-tailed Deer. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-2012-08-11676

Chicago Manual of Style (16^th Edition):

Fisher, Amanda. “Morphological and Genetic Comparisons between Babesia bovis and Trypanosoma spp. Found in Cattle and White-tailed Deer.” 2012. Masters Thesis, Texas A&M University. Accessed March 08, 2021. http://hdl.handle.net/1969.1/ETD-TAMU-2012-08-11676.

MLA Handbook (7^th Edition):

Fisher, Amanda. “Morphological and Genetic Comparisons between Babesia bovis and Trypanosoma spp. Found in Cattle and White-tailed Deer.” 2012. Web. 08 Mar 2021.

Vancouver:

Fisher A. Morphological and Genetic Comparisons between Babesia bovis and Trypanosoma spp. Found in Cattle and White-tailed Deer. [Internet] [Masters thesis]. Texas A&M University; 2012. [cited 2021 Mar 08]. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2012-08-11676.

Council of Science Editors:

Fisher A. Morphological and Genetic Comparisons between Babesia bovis and Trypanosoma spp. Found in Cattle and White-tailed Deer. [Masters Thesis]. Texas A&M University; 2012. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2012-08-11676

Portland State University

2. Bennett, J. Lindsley. The electrokaryotype and molecular characterization of Trypanosoma cervi isolates using recombinant DNA techniques.

Degree: MS(M.S.) in Biology, Biology, 1990, Portland State University

URL: https://pdxscholar.library.pdx.edu/open_access_etds/4024

► The distribution of trypanosomes infecting wild ruminants of North America has only recently been investigated . Many isolates have been mensurally studied and were… (more)

Subjects/Keywords: Trypanosoma cervi; Recombinant DNA; Biology

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APA (6^th Edition):

Bennett, J. L. (1990). The electrokaryotype and molecular characterization of Trypanosoma cervi isolates using recombinant DNA techniques. (Masters Thesis). Portland State University. Retrieved from https://pdxscholar.library.pdx.edu/open_access_etds/4024

Chicago Manual of Style (16^th Edition):

Bennett, J Lindsley. “The electrokaryotype and molecular characterization of Trypanosoma cervi isolates using recombinant DNA techniques.” 1990. Masters Thesis, Portland State University. Accessed March 08, 2021. https://pdxscholar.library.pdx.edu/open_access_etds/4024.

MLA Handbook (7^th Edition):

Bennett, J Lindsley. “The electrokaryotype and molecular characterization of Trypanosoma cervi isolates using recombinant DNA techniques.” 1990. Web. 08 Mar 2021.

Vancouver:

Bennett JL. The electrokaryotype and molecular characterization of Trypanosoma cervi isolates using recombinant DNA techniques. [Internet] [Masters thesis]. Portland State University; 1990. [cited 2021 Mar 08]. Available from: https://pdxscholar.library.pdx.edu/open_access_etds/4024.

Council of Science Editors:

Bennett JL. The electrokaryotype and molecular characterization of Trypanosoma cervi isolates using recombinant DNA techniques. [Masters Thesis]. Portland State University; 1990. Available from: https://pdxscholar.library.pdx.edu/open_access_etds/4024

3. Salmijärvi, Sari. Genetisk artbestämning och karaktärisering av Trypanosoma theileri.

Degree: Sustainable Development of Society and Technology, 2008, Mälardalen UniversityMälardalen University

URL: http://urn.kb.se/resolve?urn=urn:nbn:se:mdh:diva-4313

Subjects/Keywords: Biologi; bioteknik; Trypanosoma theileri; DNA; Biology; Biologi

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APA (6^th Edition):

Salmijärvi, S. (2008). Genetisk artbestämning och karaktärisering av Trypanosoma theileri. (Thesis). Mälardalen UniversityMälardalen University. Retrieved from http://urn.kb.se/resolve?urn=urn:nbn:se:mdh:diva-4313

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Salmijärvi, Sari. “Genetisk artbestämning och karaktärisering av Trypanosoma theileri.” 2008. Thesis, Mälardalen UniversityMälardalen University. Accessed March 08, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:mdh:diva-4313.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Salmijärvi, Sari. “Genetisk artbestämning och karaktärisering av Trypanosoma theileri.” 2008. Web. 08 Mar 2021.

Vancouver:

Salmijärvi S. Genetisk artbestämning och karaktärisering av Trypanosoma theileri. [Internet] [Thesis]. Mälardalen UniversityMälardalen University; 2008. [cited 2021 Mar 08]. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:mdh:diva-4313.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Salmijärvi S. Genetisk artbestämning och karaktärisering av Trypanosoma theileri. [Thesis]. Mälardalen UniversityMälardalen University; 2008. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:mdh:diva-4313

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

4. Calderano, Simone Guedes. Alternativas da replicação do DNA: vias de controle e dinâmica das forquilhas em trypanosomas.

Degree: PhD, Biologia da Relação Patógeno-Hospedeiro, 2013, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/42/42135/tde-03062014-175441/ ;

►

A replicação do DNA tem início nas origens de replicação que são licenciadas na transição das fases M/G1, pelo complexo de pré-replicação (CPR), e ativadas… (more)

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A replicação do DNA tem início nas origens de replicação que são licenciadas na transição das fases M/G1, pelo complexo de pré-replicação (CPR), e ativadas apenas na fase S. Existem diversas origens de replicação no genoma, mas apenas parte destas origens é disparada em diferentes momentos de S, havendo assim origens early (disparadas no início de S) e late (disparadas mais tardiamente). Em trypanosomas as origens de replicação são reconhecidas por um CPR formado por Orc1/Cdc6 e pelo complexo MCM2-7. Em T. cruzi observamos que existem dois mecanismos diferentes para controlar a replicação do DNA. Durante o ciclo celular da forma epimastigota, as proteínas do CPR são sempre expressas e ligadas ao DNA, mas durante o ciclo de vida Orc1/Cdc6 se liga ao DNA apenas nas formas que replicam, e Mcm7 não é expressa nas que não replicam. Também foi analisado o perfil das forquilhas de replicação em T. brucei utilizando a técnica de SMARD onde vimos que a velocidade da forquilha é semelhante a dos demais eucariontes, além de encontrarmos a primeira origem de replicação late.

The DNA replication starts at the origins of replication, which are licensed at M/G1 transition, by the pre replication complex (PRC), and are activated just at S phase. There are many origins of replication along genome, but some of them are fired at different moments of S phase. So there are early and late origins fired at the beginning or later in S phase, respectively. The PRC of trypanosomes is composed of Orc1/Cdc6 and Mcm2-7. We could observe that in T. cruzi there are two distinct ways to control DNA replication. Whereas in epimastigote cell cycle the PRC are expressed and bound to DNA in all phases, during T. cruzi life cycle Orc1/Cdc6 is bound to DNA only in replicative forms and Mcm7 is absent in the non-replicative forms. We also analyzed the fork profile in T. brucei through SMARD technique. We found that the speed of replication fork is similar from other eukaryotes and that different replication origins are fired every cell cycle. Finally, we found a new origin of replication that is the first late origin described in this organism.

Advisors/Committee Members: Sabbaga, Maria Carolina Quartim Barbosa Elias.

Subjects/Keywords: Trypanosoma cruzi; Trypanosoma cruzi; Trypanosoma; Trypanosoma; DNA replication; Replicação do DNA

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APA (6^th Edition):

Calderano, S. G. (2013). Alternativas da replicação do DNA: vias de controle e dinâmica das forquilhas em trypanosomas. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/42/42135/tde-03062014-175441/ ;

Chicago Manual of Style (16^th Edition):

Calderano, Simone Guedes. “Alternativas da replicação do DNA: vias de controle e dinâmica das forquilhas em trypanosomas.” 2013. Doctoral Dissertation, University of São Paulo. Accessed March 08, 2021. http://www.teses.usp.br/teses/disponiveis/42/42135/tde-03062014-175441/ ;.

MLA Handbook (7^th Edition):

Calderano, Simone Guedes. “Alternativas da replicação do DNA: vias de controle e dinâmica das forquilhas em trypanosomas.” 2013. Web. 08 Mar 2021.

Vancouver:

Calderano SG. Alternativas da replicação do DNA: vias de controle e dinâmica das forquilhas em trypanosomas. [Internet] [Doctoral dissertation]. University of São Paulo; 2013. [cited 2021 Mar 08]. Available from: http://www.teses.usp.br/teses/disponiveis/42/42135/tde-03062014-175441/ ;.

Council of Science Editors:

Calderano SG. Alternativas da replicação do DNA: vias de controle e dinâmica das forquilhas em trypanosomas. [Doctoral Dissertation]. University of São Paulo; 2013. Available from: http://www.teses.usp.br/teses/disponiveis/42/42135/tde-03062014-175441/ ;

University of Nairobi

5. Leunita, SA. Mechanical transmission of trypanosoma evansi and trypanosoma congolense by African stomoxys species.

Degree: 1997, University of Nairobi

URL: http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/25050

► Simulated mechanical transmission rates of T. congolense and T. evansi were studied using wild and blood-fed S. n. niger, S. taeniatus, and laboratory reared teneral… (more)

Subjects/Keywords: Trypanosoma

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APA (6^th Edition):

Leunita, S. (1997). Mechanical transmission of trypanosoma evansi and trypanosoma congolense by African stomoxys species. (Thesis). University of Nairobi. Retrieved from http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/25050

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Leunita, SA. “Mechanical transmission of trypanosoma evansi and trypanosoma congolense by African stomoxys species. ” 1997. Thesis, University of Nairobi. Accessed March 08, 2021. http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/25050.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Leunita, SA. “Mechanical transmission of trypanosoma evansi and trypanosoma congolense by African stomoxys species. ” 1997. Web. 08 Mar 2021.

Vancouver:

Leunita S. Mechanical transmission of trypanosoma evansi and trypanosoma congolense by African stomoxys species. [Internet] [Thesis]. University of Nairobi; 1997. [cited 2021 Mar 08]. Available from: http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/25050.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Leunita S. Mechanical transmission of trypanosoma evansi and trypanosoma congolense by African stomoxys species. [Thesis]. University of Nairobi; 1997. Available from: http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/25050

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

6. Canavaci, Adriana Monte Cassiano. Papel dos leucotrienos durante a infecção experimental de camundongos com \'Trypanosoma cruzi\'.

Degree: Mestrado, Biociências Aplicadas à Farmácia, 2007, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/60/60135/tde-27032009-152740/ ;

►

No presente trabalho verificamos o papel dos Leucotrienos na modulação da resposta imune durante a fase aguda da infecção experimental pelo Trypanosoma cruzi, usando como… (more)

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No presente trabalho verificamos o papel dos Leucotrienos na modulação da resposta imune durante a fase aguda da infecção experimental pelo Trypanosoma cruzi, usando como modelo camundongos deficientes da enzima 5-lipoxigenase (5-LOko). Os nossos dados demonstram que camundongos infectados pelo T. cruzi produzem metabólitos do ácido aracdônico como PGE2, LTB4 e LTC4. Comparados aos animais controles, os animais 5-LOko apresenta parasitemia mais tardia e menor, tem menor parasitismo tissular, menor infiltrado de células inflamatórias no coração e musculatura esquelética e apresenta menor taxa de mortalidade durante a fase aguda, indicando que animais deficientes de leucotrienos são mais resistentes a infecção pelo parasito. Animais 5-LOko está relacionado com a manutenção de números elevados de células F4/80+ e redução de células CD11b+ durante a infecção e menor número de células T ativadas expressando os marcadores CD4+CD69+, CD4+CD25+, CD4+CD44+ e CD8+CD69+, números inalterados de células T regulatórias CD4+CD25+GITR+ e menor produção de anticorpos parasito-específicos do isotipo IgG2a. O controle eficiente de parasitas por animais 5-LOko está associado ao aumento de células Gr-1+ e CD11c+GR-1+, produção aumentada IL-12, IFN-g, e produzirem menos PGE2, IL-10, ao contrario, animais controles, incapazes de controlar parasitas circulantes, produzem mais PGE2 e IL-10 e menos IL-12 e IFN-g. A baixa mortalidade de animais 5-LOko correlaciona com a produção de PGE2 e IL-10, produzir muita IL-12 e menos IFN-g e NO e baixíssima parasitemia. A mortalidade maior de animais controles envolve a produção IFN-g e altos níveis de LTB4, LTC4, NO e ausência de IL-10, IL-1b, PGE2 e números elevados de parasitas circulantes. Ainda macrófagos de animais 5-LOko apresentam maior capacidade de adesão/internalização de tripomastigotas e alta atividade tripanocida por mecanismo independente da geração de NO. Estes dados em conjunto demonstram que mediadores lipídicos produzidos pela enzima 5-lipoxigenase como LTB4 e LTC4 modulam negativamente a capacidade dos camundongos para geração de uma resposta imune capaz de controlar os parasitos durante a fase aguda da infecção pelo T. cruzi.

Accumulating studies have indicated that 5-lipoxigenase (5-LO) converted lipid mediators as leukotrienes acts modulating the host immune response against infectious diseases. The precise role of leukotrienes during the protozoan infection is unknown. In this work we evaluate the role of leukotrienes during the acute phase of Trypanosoma cruzi infection using as model the 5-lypoxigenase deficient mice (5-LOko). Our results show that PGE2, LTB4 and LTC4 are produced during the Trypanosoma cruzi infection. 5-LOko infected mice are more resistant than control mice as judge by the lower parasitemia, decreased number of parasite nest and inflammatory cells in the heart and skeletal muscle and low rate of mortality. The resistance of 5-LOko mice is associated with the increased capacity of spleen cells to produce cytokines as IL-12 and IFN-g; sustained capacity to…

Advisors/Committee Members: Nomizo, Auro.

Subjects/Keywords: Imunorregulação; Leucotrienos; leukotrienes; Trypanosoma cruz; Trypanosoma cruzi

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Canavaci, A. M. C. (2007). Papel dos leucotrienos durante a infecção experimental de camundongos com \'Trypanosoma cruzi\'. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/60/60135/tde-27032009-152740/ ;

Chicago Manual of Style (16^th Edition):

Canavaci, Adriana Monte Cassiano. “Papel dos leucotrienos durante a infecção experimental de camundongos com \'Trypanosoma cruzi\'.” 2007. Masters Thesis, University of São Paulo. Accessed March 08, 2021. http://www.teses.usp.br/teses/disponiveis/60/60135/tde-27032009-152740/ ;.

MLA Handbook (7^th Edition):

Canavaci, Adriana Monte Cassiano. “Papel dos leucotrienos durante a infecção experimental de camundongos com \'Trypanosoma cruzi\'.” 2007. Web. 08 Mar 2021.

Vancouver:

Canavaci AMC. Papel dos leucotrienos durante a infecção experimental de camundongos com \'Trypanosoma cruzi\'. [Internet] [Masters thesis]. University of São Paulo; 2007. [cited 2021 Mar 08]. Available from: http://www.teses.usp.br/teses/disponiveis/60/60135/tde-27032009-152740/ ;.

Council of Science Editors:

Canavaci AMC. Papel dos leucotrienos durante a infecção experimental de camundongos com \'Trypanosoma cruzi\'. [Masters Thesis]. University of São Paulo; 2007. Available from: http://www.teses.usp.br/teses/disponiveis/60/60135/tde-27032009-152740/ ;

7. Márden Estevão Mattos Júnior. Influência do parasito e do hospedeiro no potencial patogênico de populações de Trypanosoma cruzi I na fase aguda da infecção experimental em camundongos.

Degree: 2010, Universidade Federal do Triângulo Mineiro

URL: http://bdtd.uftm.edu.br/tde_busca/arquivo.php?codArquivo=138

►

The populations of Trypanosoma cruzi (Tc) show high biological and genetic variability and are classified into six groups TcI - TcVI. Currently, data concerning the… (more)

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The populations of Trypanosoma cruzi (Tc) show high biological and genetic variability and are classified into six groups TcI - TcVI. Currently, data concerning the pathogenicity of the TcI are conflicting and may be due to host genetic component and spread to humans in populations of T. cruzi restricted to a wild environment. However, the insertion of new vectors in the domestic environment enables contact T. cruzi wild with new hosts, increasing the genotypic variability by modifying their pathogenic potential. The study of biological behavior was conducted using groups of 15 Swiss, Balb/c and C57BL/6 male mice that were infected intraperitoneally with 1 x 104 trypomastigotes to evaluate the infectivity, virulence, blood and tissue parasitism, pathogenicity, tissue tropism and genetic variability of the LSSP-PCR (Low Stringency Single Specific Primer - PCR). We used four samples of T. cruzi isolates from triatomines in different Brazilian regions properly cryopreserved in culture and subsequently reactivated; isolates ALVANI, AQ1-7 and MUTUM classified as TcI and PV isolated as TcIV. In relation to the biological study, the TcI isolates showed parasitemia subpatent, and a variation in the infectivity and pathogenic potential followed by a low virulence in the acute phase by a 100% survival of animals. The isolates ALVANI and AQ1-7 were considered pathogenic, but with low infectivity, whereas, MUTUM and PV (TcIV) showed the high infectivity and low pathogenicity. The tissue tropism presenting by isolates of TcI was smooth and striated muscle, however, the isolated PV showed tropism for the heart. The results related to the genetic component demonstrated the existence of a large genetic variability among populations of blood and tissue, while the populations of the inoculum, and blood culture showed a significant intra-specific homogeneity related to the four isolates of T. cruzi, showing no differences between strains of mice. Most of the tissues analyzed inter-specific manner was grouped according to isolate analyzed showing the maintenance of characteristics within each population. TcI strains differences could be attributed to the polar populations associated with and without the capacity to produce lesions. These facts may explain the clinical controversies found in TcI human infections in different endemic regions.

As populações do Trypanosoma cruzi (Tc) apresentam elevada variabilidade biológica e genética e são classificadas em seis grupos TcI a TcVI. Atualmente, os dados referentes à patogenicidade do TcI são conflitantes e podem decorrer do componente genético do hospedeiro e da propagação ao homem de populações de T. cruzi restritas ao ambiente silvestre. Contudo, a inserção de novos vetores no ambiente doméstico possibilita o contato do T. cruzi silvestre com novos hospedeiros, elevando a variabilidade genotípica, modificando seu potencial patogênico. O estudo do comportamento biológico foi realizado utilizando grupos de 15 camundongos Swiss, e duas linhagens isogênicas Balb/c e C57BL/6, machos, que…

Advisors/Committee Members: Eliane Lages Silva.

Subjects/Keywords: Trypanosoma cruzi; Tripanossomose; MEDICINA; Trypanosoma cruzi; Trypanosomiasis

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APA (6^th Edition):

Júnior, M. E. M. (2010). Influência do parasito e do hospedeiro no potencial patogênico de populações de Trypanosoma cruzi I na fase aguda da infecção experimental em camundongos. (Thesis). Universidade Federal do Triângulo Mineiro. Retrieved from http://bdtd.uftm.edu.br/tde_busca/arquivo.php?codArquivo=138

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Júnior, Márden Estevão Mattos. “Influência do parasito e do hospedeiro no potencial patogênico de populações de Trypanosoma cruzi I na fase aguda da infecção experimental em camundongos.” 2010. Thesis, Universidade Federal do Triângulo Mineiro. Accessed March 08, 2021. http://bdtd.uftm.edu.br/tde_busca/arquivo.php?codArquivo=138.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Júnior, Márden Estevão Mattos. “Influência do parasito e do hospedeiro no potencial patogênico de populações de Trypanosoma cruzi I na fase aguda da infecção experimental em camundongos.” 2010. Web. 08 Mar 2021.

Vancouver:

Júnior MEM. Influência do parasito e do hospedeiro no potencial patogênico de populações de Trypanosoma cruzi I na fase aguda da infecção experimental em camundongos. [Internet] [Thesis]. Universidade Federal do Triângulo Mineiro; 2010. [cited 2021 Mar 08]. Available from: http://bdtd.uftm.edu.br/tde_busca/arquivo.php?codArquivo=138.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Júnior MEM. Influência do parasito e do hospedeiro no potencial patogênico de populações de Trypanosoma cruzi I na fase aguda da infecção experimental em camundongos. [Thesis]. Universidade Federal do Triângulo Mineiro; 2010. Available from: http://bdtd.uftm.edu.br/tde_busca/arquivo.php?codArquivo=138

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Aberdeen

8. Aboubaker, Eltayb Abdelwahab Mohamed. Trypanosomiasis : molecular diagnosis of Trypanosoma evansi infection and endotoxaemia during Trypanosoma brucei rhodesiense infection.

Degree: PhD, 2017, University of Aberdeen

URL: https://abdn.alma.exlibrisgroup.com/view/delivery/44ABE_INST/12152979550005941 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.715461

► Two aspects of trypanosomiasis have been investigated in this study. First, molecular methods were applied to the diagnosis of T.evansi in camels in South Libya.… (more)

▼ Two aspects of trypanosomiasis have been investigated in this study. First, molecular methods were applied to the diagnosis of T.evansi in camels in South Libya. The aim of the study was to determine if FTA card blood sampling and PCR amplification could detect parasites and this be used as tool for diagnosis and epidemiology. Targeted samples of 70 camels were identified on the basis of symptoms of infection and blood was collected on FTA cards. PCR primers and conditions for the amplification of T.evansi DNA were developed on the basis of the literature and a positive control clone grown in the laboratory. The assay found 84.3% of camel samples positive using TBR primers (177bp amplicon) and ITS nested primers (611-1513bp amplicons). This result demonstrated that Surra is endemic in this area, and that T.evansi was the species that was involved. The ITS and TBR loci in the parasites identified in Libya were almost identical to those previously reported in the genbank database, though with some polymorphisms. Dullness and emaciation were the clinical signs of camels infected by trypanosomes, and these two symptoms were significantly related to the 1200bp ITS nested PCR amplicon. These two symptoms can be thus used as a sign an initial diagnosis of T.evansi infection in camels. The second aspect of trypanosomiasis studied was the occurrence of endotoxaemia in infection. The first part of this research investigated endotoxin levels in clinical human African trypasnosomiasis using the Limulus Amoebocyte lysate assay. Endotoxin levels were significantly increased over control individuals in the plasma of T.b.rhodesiense patients. This endotoxaemia was unrelated to infection duration, parasitaemia or clinical stage but resolved after clearance of parasites by drug treatment. In the cerebrospinal fluid there was no significant difference in endotoxin level between early and late stage cases and no relationship to parasite loads. It is argued on the basis of the data that endotoxaemia in trypanosomiasis most likely results from increases in permeability of the gut to endotoxins from gram negative enter bacteria. This conclusion was further supported from a study using cell culture adapted T.brucei and secreted products which gave no evidence of any endotoxin activity. Also samples of an acute experimental mouse infection with T.brucei gave no endotoxin activity, suggesting that this phenomenon requires a more chronic infection in mice. No relationships were found between plasma or CSF endotoxin levels to neurological signs of infection. However the presence of a gross inflammatory clinical symptom, splenomegaly, was associated with endotoxaemia and the concentrations of 3 plasma cytokines associated with the immune response in trypanosome infection were associated with correlated to plasma endotoxin levels. In order to determine the nature of the endotoxin activity, a biosensor cell assay for LPS was used, based on human embryonic kidney cells transfected with TLR4/MD3 and a NF-κB induced alkaline phosphatase reporter…

Subjects/Keywords: 616.9; Trypanosoma; Trypanosoma brucei; Trypanosomiasis; Endotoxemia

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Aboubaker, E. A. M. (2017). Trypanosomiasis : molecular diagnosis of Trypanosoma evansi infection and endotoxaemia during Trypanosoma brucei rhodesiense infection. (Doctoral Dissertation). University of Aberdeen. Retrieved from https://abdn.alma.exlibrisgroup.com/view/delivery/44ABE_INST/12152979550005941 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.715461

Chicago Manual of Style (16^th Edition):

Aboubaker, Eltayb Abdelwahab Mohamed. “Trypanosomiasis : molecular diagnosis of Trypanosoma evansi infection and endotoxaemia during Trypanosoma brucei rhodesiense infection.” 2017. Doctoral Dissertation, University of Aberdeen. Accessed March 08, 2021. https://abdn.alma.exlibrisgroup.com/view/delivery/44ABE_INST/12152979550005941 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.715461.

MLA Handbook (7^th Edition):

Aboubaker, Eltayb Abdelwahab Mohamed. “Trypanosomiasis : molecular diagnosis of Trypanosoma evansi infection and endotoxaemia during Trypanosoma brucei rhodesiense infection.” 2017. Web. 08 Mar 2021.

Vancouver:

Aboubaker EAM. Trypanosomiasis : molecular diagnosis of Trypanosoma evansi infection and endotoxaemia during Trypanosoma brucei rhodesiense infection. [Internet] [Doctoral dissertation]. University of Aberdeen; 2017. [cited 2021 Mar 08]. Available from: https://abdn.alma.exlibrisgroup.com/view/delivery/44ABE_INST/12152979550005941 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.715461.

Council of Science Editors:

Aboubaker EAM. Trypanosomiasis : molecular diagnosis of Trypanosoma evansi infection and endotoxaemia during Trypanosoma brucei rhodesiense infection. [Doctoral Dissertation]. University of Aberdeen; 2017. Available from: https://abdn.alma.exlibrisgroup.com/view/delivery/44ABE_INST/12152979550005941 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.715461

9. Miriam Pires de Castro Oliveira. Trypanosoma dionisii: determinação do padrão de expressão de epítopos definidos por anticorpos monoclonais anti- Trypanosoma cruzi e estudos da invasão em linhagens celulares.

Degree: 2008, Universidade Federal de São Paulo

URL: http://www.bdtd.unifesp.br/tede//tde_busca/arquivo.php?codArquivo=2552 ; http://www.bdtd.unifesp.br/tede//tde_busca/arquivo.php?codArquivo=2553 ; http://www.bdtd.unifesp.br/tede//tde_busca/arquivo.php?codArquivo=2554

► Trypanosoma dionisii é um tripanosomatídeo de morcegos filogeneticamente muito próximo ao Trypanosoma cruzi, agente etiológico da Doença de Chagas que, segundo a Organização Mundial de… (more)

▼ Trypanosoma dionisii é um tripanosomatídeo de morcegos filogeneticamente muito próximo ao Trypanosoma cruzi, agente etiológico da Doença de Chagas que, segundo a Organização Mundial de Saúde, afeta 18 milhões de pessoas na América Latina além de ocorrer aproximadamente 300.000 novas infecções anualmente. Durante seu ciclo de vida, T. dionisii passa por diferentes formas evolutivas alternando entre hospedeiro vertebrado e invertebrado. As formas são: epimastigotas e tripomastigotas metacíclicas no hospedeiro invertebrado e tripomastigotas sanguíneas e amastigotas no hospedeiro vertebrado. As formas tripomastigotas metacíclicas são capazes de invadir e se multiplicar in vitro em diversos tipos de células de mamíferos. O presente trabalho teve como objetivo observar características da ultraestrutura da espécie, alguns aspectos de seu mecanismo de invasão e identificar possível compartilhamento de epítopos entre as espécies T. dionisii e T. cruzi através de ensaios utilizando anticorpos monoclonais anti-T. cruzi. Assim como ocorre com T. cruzi, as formas tripomastigotas metacíclicas de T. dionisii recrutam lisossomos durante a invasão da célula hospedeira e permanecem em vacúolos LAMP-1 positivos por várias horas. Nossos resultados mostraram que aproximadamente 10% das formas tripomastigotas metacíclicas LAMP-1 positivas de T. dionisii, transformam-se em amastigotas em compartimentos LAMP-1 positivos, permanecendo nestes vacúolos por até 96 horas. Além disto, o pré-tratamento da célula hospedeira com nocodazol (composto que despolimeriza microtúbulos, impedindo o recrutamento lisossomal) não alterou a invasão do parasita, enquanto o prétratamento com wortmanina (composto que inibe a invasão via PI3-quinase e invaginação de membrana) inibiu significativamente a invasão. Estes dados sugerem que a fusão lisossomal talvez não seja um evento crítico para o estabelecimento de uma infecção bem sucedida de T. dionisii. A análise da expressão de epítopos definidos por anticorpos monoclonais anti-amastigotas de T. cruzi mostra uma maior proximidade imuno-reativa entre T. dionisii e T. cruzi da cepa G, pois T. dionisii reagiu com apenas um dos anticorpos anti-T. cruzi da cepa CL testados. Quanto à ultraestrutura T. dionisii é bastante semelhante a T. cruzi, com exceção do núcleo, que em T. dionisii apresenta um característico padrão de condensação da cromatina. Advisors/Committee Members: Renato Arruda Mortara, Rafael Miyazawa Martins, Luciana de Oliveira Andrade, Marta Maria Geraldes Teixeira.

Subjects/Keywords: Trypanosoma; Epitopos; MICROBIOLOGIA

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Oliveira, M. P. d. C. (2008). Trypanosoma dionisii: determinação do padrão de expressão de epítopos definidos por anticorpos monoclonais anti- Trypanosoma cruzi e estudos da invasão em linhagens celulares. (Thesis). Universidade Federal de São Paulo. Retrieved from http://www.bdtd.unifesp.br/tede//tde_busca/arquivo.php?codArquivo=2552 ; http://www.bdtd.unifesp.br/tede//tde_busca/arquivo.php?codArquivo=2553 ; http://www.bdtd.unifesp.br/tede//tde_busca/arquivo.php?codArquivo=2554

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Oliveira, Miriam Pires de Castro. “Trypanosoma dionisii: determinação do padrão de expressão de epítopos definidos por anticorpos monoclonais anti- Trypanosoma cruzi e estudos da invasão em linhagens celulares.” 2008. Thesis, Universidade Federal de São Paulo. Accessed March 08, 2021. http://www.bdtd.unifesp.br/tede//tde_busca/arquivo.php?codArquivo=2552 ; http://www.bdtd.unifesp.br/tede//tde_busca/arquivo.php?codArquivo=2553 ; http://www.bdtd.unifesp.br/tede//tde_busca/arquivo.php?codArquivo=2554.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Oliveira, Miriam Pires de Castro. “Trypanosoma dionisii: determinação do padrão de expressão de epítopos definidos por anticorpos monoclonais anti- Trypanosoma cruzi e estudos da invasão em linhagens celulares.” 2008. Web. 08 Mar 2021.

Vancouver:

Oliveira MPdC. Trypanosoma dionisii: determinação do padrão de expressão de epítopos definidos por anticorpos monoclonais anti- Trypanosoma cruzi e estudos da invasão em linhagens celulares. [Internet] [Thesis]. Universidade Federal de São Paulo; 2008. [cited 2021 Mar 08]. Available from: http://www.bdtd.unifesp.br/tede//tde_busca/arquivo.php?codArquivo=2552 ; http://www.bdtd.unifesp.br/tede//tde_busca/arquivo.php?codArquivo=2553 ; http://www.bdtd.unifesp.br/tede//tde_busca/arquivo.php?codArquivo=2554.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Oliveira MPdC. Trypanosoma dionisii: determinação do padrão de expressão de epítopos definidos por anticorpos monoclonais anti- Trypanosoma cruzi e estudos da invasão em linhagens celulares. [Thesis]. Universidade Federal de São Paulo; 2008. Available from: http://www.bdtd.unifesp.br/tede//tde_busca/arquivo.php?codArquivo=2552 ; http://www.bdtd.unifesp.br/tede//tde_busca/arquivo.php?codArquivo=2553 ; http://www.bdtd.unifesp.br/tede//tde_busca/arquivo.php?codArquivo=2554

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidad de Chile

10. Sosoniuk Roche, Eduardo Rodrigo. Calreticulina de trypanosoma cruzi : un factor de virulencia que unido exógenamente a epimastigotes, promueve su penetración a células hospederas.

Degree: 2013, Universidad de Chile

URL: http://repositorio.uchile.cl/handle/2250/115053

► La enfermedad de Chagas es una patología endémica en América Latina y sin un tratamiento eficaz, causada por el protozoo hemoflagelado Trypanosoma cruzi (T. cruzi).… (more)

▼ La enfermedad de Chagas es una patología endémica en América Latina y sin un tratamiento eficaz, causada por el protozoo hemoflagelado Trypanosoma cruzi (T. cruzi). Es un problema mayor de salud identificándose actualmente entre 8 a 9 millones de infectados, de los cuales aproximadamente un 30% desarrolla sintomatología. Sin embargo, la infección se ha globalizado durante los últimos años, confirmándose sobre 300.000 sero-positivos en Estados Unidos y varias decenas de miles en Europa, Asia y Oceanía. Esto se debe a que, aunque T. cruzi es normalmente transmitido por insectos triatominios infectados, se ha descrito la transmisión por transfusión sanguínea, trasplante de órganos, infección congénita y consumo de alimentos contaminados con el parásito. En el ciclo de vida de T. cruzi se pueden identificar 3 etapas principales: amastigote y epimastigote, que cumplen funciones replicativas en el hospedero mamífero y en el insecto vector respectivamente, y la forma tripomastigote, encargada de infectar células eucariontes. Calreticulina de T. cruzi (TcCRT), es una proteína de 45 kDa, aislada, clonada y caracterizada en el Laboratorio de Inmunología de la Agresión Microbiana (LIAM), de la Facultad de Medicina de nuestra Universidad. Se ha demostrado que TcCRT es translocada a la zona de emergencia flagelar de los tripomastigotes, donde captura C1, primer componente de la vía clásica del sistema del complemento. En el humano, esta señal es característica de células apoptóticas. Es por esto que al capturar C1, se reclutan macrófagos para fagocitar a los tripomastigotes. Una vez dentro de estas células, el parásito escapa de la vacuola parasitófora, y comienza a diferenciarse, iniciando el proceso de infección del huésped. Junto a esto, la interacción entre TcCRT y C1 impide la activación del sistema del complemento del hospedero, disminuyendo la lisis del parásito por este medio. Resultados obtenidos previamente en LIAM muestran que TcCRT se expresa marginalmente en la membrana plasmática de la forma epimastigote del clon Dm28c. En base a esto, se propuso como Hipótesis de Trabajo de esta Memoria de Título, que, al incubar epimastigotes con rTcCRT, se promueve su penetración a las células hospederas mamíferas. Los principales resultados obtenidos señalan que: i) epimastigotes unen rTcCRT en su membrana, posiblemente mediante alguna proteína que sirva de anclaje, ii) el tratamiento con rTcCRT en presencia o ausencia de C1q conlleva a un aumento en la penetración del parásito en fibroblastos que expresan CRT, iii) la ausencia de CRT en la membrana de la célula hospedera lleva a una disminución en la penetración de los parásitos. En el contexto del epimastigote, puede que la falta de translocación de TcCRT sea el factor principal para que esta forma presente menor resistencia a lisis por el complemento y falle en desarrollar el ciclo infectivo en el mamífero; Chagas’ disease is an endemic pathology in Latin America, caused by the hemoflagelated protozoan Trypanosoma cruzi (T. cruzi), and still with no available effective…

Subjects/Keywords: Calreticulina; Trypanosoma cruzi

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sosoniuk Roche, E. R. (2013). Calreticulina de trypanosoma cruzi : un factor de virulencia que unido exógenamente a epimastigotes, promueve su penetración a células hospederas. (Thesis). Universidad de Chile. Retrieved from http://repositorio.uchile.cl/handle/2250/115053

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Sosoniuk Roche, Eduardo Rodrigo. “Calreticulina de trypanosoma cruzi : un factor de virulencia que unido exógenamente a epimastigotes, promueve su penetración a células hospederas.” 2013. Thesis, Universidad de Chile. Accessed March 08, 2021. http://repositorio.uchile.cl/handle/2250/115053.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Sosoniuk Roche, Eduardo Rodrigo. “Calreticulina de trypanosoma cruzi : un factor de virulencia que unido exógenamente a epimastigotes, promueve su penetración a células hospederas.” 2013. Web. 08 Mar 2021.

Vancouver:

Sosoniuk Roche ER. Calreticulina de trypanosoma cruzi : un factor de virulencia que unido exógenamente a epimastigotes, promueve su penetración a células hospederas. [Internet] [Thesis]. Universidad de Chile; 2013. [cited 2021 Mar 08]. Available from: http://repositorio.uchile.cl/handle/2250/115053.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Sosoniuk Roche ER. Calreticulina de trypanosoma cruzi : un factor de virulencia que unido exógenamente a epimastigotes, promueve su penetración a células hospederas. [Thesis]. Universidad de Chile; 2013. Available from: http://repositorio.uchile.cl/handle/2250/115053

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidad de Chile

11. Ponce López, Iván Alexis. Identificación y caracterización de la enzima flap endonucleasa de trypanosoma cruzi (TcFEN1) : su rol en la proliferación del parásito y su resistencia frente a daño oxidativo.

Degree: 2017, Universidad de Chile

URL: http://repositorio.uchile.cl/handle/2250/165732

► Trypanosoma cruzi (T. cruzi) es un protozoo parásito, agente causal de la enfermedad de Chagas, endémica en el continente americano. Presenta un ciclo de vida… (more)

▼ Trypanosoma cruzi (T. cruzi) es un protozoo parásito, agente causal de la enfermedad de Chagas, endémica en el continente americano. Presenta un ciclo de vida complejo, infectando tanto hospederos mamíferos como insectos triatominos (hematófagos) y presentando cuatro formas celulares: tripomastigotes sanguíneos, epimastigotes, amastigotes y tripomastigotes metacíclicos. En humanos, la infección por T. cruzi en etapas avanzadas puede provocar serios trastornos cardíacos y digestivos, con un alto costo médico, y que pueden ser mortales de no ser tratados adecuadamente. Hasta el momento, no existe una cura definitiva para la infección crónica por T. cruzi. Este parásito sobrevive al efecto de especies reactivas de oxígeno y nitrógeno (ROS/RNS) generadas tanto por células del hospedero mamífero como por la digestión de la sangre en el insecto hematófago. Se ha propuesto que estas especies reactivas dañan el DNA del parásito y que éste sobrevive activando el mecanismo de reparación del DNA por escisión de bases (vía BER). Este es un proceso muy conservado, que presenta dos sub-vías: la vía corta, en la que se reemplaza un nucleótido, o la vía larga, donde se insertan dos o más nucleótidos. En esta vía de reparación del DNA participan distintas enzimas, como DNA glicosilasas, AP endonucleasas (APE), DNA polimerasas, flap endonucleasas (FEN1) y DNA ligasas. Hasta el momento, se ha demostrado que: 1) el DNA del parásito es dañado por ROS/RNS; 2) T. cruzi tiene la capacidad de reparar el daño producido por estas especies reactivas; 3) metoxiamina, un inhibidor de AP endonucleasas, aumenta la muerte celular provocada por ROS/RNS; 4) el parásito tiene dos DNA glicosilasas y dos AP endonucleasas activas. A la fecha de realización de esta Tesis no se había definido la presencia de flap endonucleasas así como de las diferentes vías (corta y/o larga) de reparación del DNA por escisión de bases en T. cruzi. Las enzimas flap endonucleasas (FEN1) se caracterizan por reconocer sustratos de DNA con estructuras específicas, que se generan en diversos procesos celulares, como la replicación del DNA o su reparación mediante la vía larga del mecanismo BER. Estudios en diversos eucariontes han demostrado su importancia en la reparación del daño oxidativo al DNA, así como en la mantención de la integridad genómica. En esta Tesis se investigó la presencia y actividad de una enzima flap endonucleasa de T. cruzi (TcFEN1), su expresión, su localización celular y su rol en la proliferación y en la sobrevida del parásito frente a condiciones de estrés oxidativo. Se demostró la presencia de actividad flap endonucleasa en distintas formas celulares de T. cruzi y se describieron algunas de sus propiedades. Paralelamente se encontró una secuencia correspondiente a una flap endonucleasa en el genoma publicado de T. cruzi, a partir de la cual se crearon plásmidos que permitieron la expresión en epimastigotes de T. cruzi de proteínas de fusión TcFEN1-GFP que también presentaron actividad flap endonucleasa y que se localizaron en el núcleo de…

Subjects/Keywords: Trypanosoma cruzi; Endonucleasas

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ponce López, I. A. (2017). Identificación y caracterización de la enzima flap endonucleasa de trypanosoma cruzi (TcFEN1) : su rol en la proliferación del parásito y su resistencia frente a daño oxidativo. (Thesis). Universidad de Chile. Retrieved from http://repositorio.uchile.cl/handle/2250/165732

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ponce López, Iván Alexis. “Identificación y caracterización de la enzima flap endonucleasa de trypanosoma cruzi (TcFEN1) : su rol en la proliferación del parásito y su resistencia frente a daño oxidativo.” 2017. Thesis, Universidad de Chile. Accessed March 08, 2021. http://repositorio.uchile.cl/handle/2250/165732.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ponce López, Iván Alexis. “Identificación y caracterización de la enzima flap endonucleasa de trypanosoma cruzi (TcFEN1) : su rol en la proliferación del parásito y su resistencia frente a daño oxidativo.” 2017. Web. 08 Mar 2021.

Vancouver:

Ponce López IA. Identificación y caracterización de la enzima flap endonucleasa de trypanosoma cruzi (TcFEN1) : su rol en la proliferación del parásito y su resistencia frente a daño oxidativo. [Internet] [Thesis]. Universidad de Chile; 2017. [cited 2021 Mar 08]. Available from: http://repositorio.uchile.cl/handle/2250/165732.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ponce López IA. Identificación y caracterización de la enzima flap endonucleasa de trypanosoma cruzi (TcFEN1) : su rol en la proliferación del parásito y su resistencia frente a daño oxidativo. [Thesis]. Universidad de Chile; 2017. Available from: http://repositorio.uchile.cl/handle/2250/165732

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidad de Chile

12. Delgadillo Liberona, José Sebastián. Clonamiento, expresión y purificación de las endonucleasas apurínicas/apirimidínicas TcAP1 y TcAP2 de Trypanosoma cruzi en condiciones nativas.

Degree: 2011, Universidad de Chile

URL: http://repositorio.uchile.cl/handle/2250/131433

► El agente causal de la enfermedad de Chagas o tripanosomiasis americana, es el parásito hemoflagelado Trypanosoma cruzi. Esta enfermedad es de carácter endémico en América… (more)

▼ El agente causal de la enfermedad de Chagas o tripanosomiasis americana, es el parásito hemoflagelado Trypanosoma cruzi. Esta enfermedad es de carácter endémico en América Latina; se estima un promedio de 10-15 millones de personas infectadas y 75-90 millones en riesgo de contraer la enfermedad. T. cruzi posee un ciclo de vida indirecto y se presenta en cuatro formas celulares; epimastigote, forma extracelular replicativa y no infectiva, tripomastigote metacíclico y tripomastigote sanguíneo, formas no replicativas e infectivas y amastigote, forma intracelular replicativa. T. cruzi es capaz de sobrevivir al daño oxidativo del DNA generado por especies reactivas de oxígeno y nitrógeno (ROS/RNS) producidas por su propio metabolismo, así como aquellas producidas en el intestino del insecto vector y en células del hospedero mamífero. Este daño sería reparado mediante la actividad de las endonucleasas apurínicas/apirimidínicas (endonucleasas AP) de la vía de escisión de bases (BER). T. cruzi presenta en su genoma secuencias que codificarían para endonucleasas AP, entre ellas TcAP1, homóloga de APE1 humana y TcAP2, homóloga de APE2 humana y de Apn2 de Schizosaccaromyces pombe. La expresión de estas proteínas podría ser fundamental para la sobrevida del parásito, tanto en el vector triatomino como en el hospedero mamífero. Para un estudio sistemático de las características de estas enzimas, se requiere obtenerlas en el más alto grado de pureza desde el parásito o por técnicas de ingeniería genética. En esta Memoria de Título se clonaron las secuencias génicas que codifican para TcAP1 y TcAP2 en vectores de expresión para células eucariontes, lo que se confirmó mediante secuenciación automática de DNA. Con estos constructos se transfectaron células S2 de Drosophila melanogaster, levaduras Pichia pastoris y epimastigotes de T. cruzi cepa Dm28c. Sorprendentemente, sólo fue posible expresar ambas endonucleasas en este último modelo. La imposibilidad de expresar ambas endonucleasas AP en los modelos P. pastoris y D. melanogaster podría relacionarse a una variedad insuficiente o limitada de ciertos tRNAs, necesarios para la expresión de TcAP1 y TcAP2, con la consecuente disminución de la traducción de los mRNAs codificantes para ambos genes. Probablemente, los tRNAs que presentan anticodones específicos para la traducción de las endonucleasas AP de T. cruzi sólo se encuentran en concentraciones adecuadas en modelos celulares de expresión filogenéticamente cercanos a este protozoario. Mediante cromatografía de afinidad se intentó purificar TcAP1-GFP a partir de homogeneizados de proteínas totales de epimastigotes transfectados que expresaban esta proteína recombinante. Sin embargo no se logró obtener la proteína recombinante en condiciones nativas de forma pura. Se concluye que la utilización de GFP asociado como proteína de fusión a TcAP1 sólo permite la identificación de la proteína recombinante y no una purificación adecuada de la misma

Subjects/Keywords: Trypanosoma cruzi; Endonucleasas

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Delgadillo Liberona, J. S. (2011). Clonamiento, expresión y purificación de las endonucleasas apurínicas/apirimidínicas TcAP1 y TcAP2 de Trypanosoma cruzi en condiciones nativas. (Thesis). Universidad de Chile. Retrieved from http://repositorio.uchile.cl/handle/2250/131433

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Delgadillo Liberona, José Sebastián. “Clonamiento, expresión y purificación de las endonucleasas apurínicas/apirimidínicas TcAP1 y TcAP2 de Trypanosoma cruzi en condiciones nativas.” 2011. Thesis, Universidad de Chile. Accessed March 08, 2021. http://repositorio.uchile.cl/handle/2250/131433.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Delgadillo Liberona, José Sebastián. “Clonamiento, expresión y purificación de las endonucleasas apurínicas/apirimidínicas TcAP1 y TcAP2 de Trypanosoma cruzi en condiciones nativas.” 2011. Web. 08 Mar 2021.

Vancouver:

Delgadillo Liberona JS. Clonamiento, expresión y purificación de las endonucleasas apurínicas/apirimidínicas TcAP1 y TcAP2 de Trypanosoma cruzi en condiciones nativas. [Internet] [Thesis]. Universidad de Chile; 2011. [cited 2021 Mar 08]. Available from: http://repositorio.uchile.cl/handle/2250/131433.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Delgadillo Liberona JS. Clonamiento, expresión y purificación de las endonucleasas apurínicas/apirimidínicas TcAP1 y TcAP2 de Trypanosoma cruzi en condiciones nativas. [Thesis]. Universidad de Chile; 2011. Available from: http://repositorio.uchile.cl/handle/2250/131433

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

13. Bech, Linda. Genetic and phenotypic characterization of trypanosomas.

Degree: Sustainable Development of Society and Technology, 2009, Mälardalen University

URL: http://urn.kb.se/resolve?urn=urn:nbn:se:mdh:diva-6435

► Trypanosoma theileri, of the subgenus Megatrypanum, a non-pathogenic cosmopolitan blood dwelling parasite of bovine. T. theileri can be cultured at room temperature in… (more)

Subjects/Keywords: trypanosoma; theileri; T.theileri; Trypanosomer; megatrypanum; PCR; cultivated; critical dry point; electron microscopy; fluorescent microscopy; Chemistry; Kemi; Biochemistry; Biokemi

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bech, L. (2009). Genetic and phenotypic characterization of trypanosomas. (Thesis). Mälardalen University. Retrieved from http://urn.kb.se/resolve?urn=urn:nbn:se:mdh:diva-6435

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Bech, Linda. “Genetic and phenotypic characterization of trypanosomas.” 2009. Thesis, Mälardalen University. Accessed March 08, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:mdh:diva-6435.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Bech, Linda. “Genetic and phenotypic characterization of trypanosomas.” 2009. Web. 08 Mar 2021.

Vancouver:

Bech L. Genetic and phenotypic characterization of trypanosomas. [Internet] [Thesis]. Mälardalen University; 2009. [cited 2021 Mar 08]. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:mdh:diva-6435.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Bech L. Genetic and phenotypic characterization of trypanosomas. [Thesis]. Mälardalen University; 2009. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:mdh:diva-6435

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Helsinki

14. Isomursu, Marja. Poron (Rangifer tarandus tarandus) loiset - kirjallisuuskatsaus.

Degree: Eläinlääketieteellinen tiedekunta; Helsingfors universitet, Veterinärmedicinska fakulteten, 1996, University of Helsinki

URL: http://hdl.handle.net/1975/1156

►

Tutkielmassa esitellään saatavilla olleen kirjallisuuden perusteella useimmat porolla esiintyvät loislajit tai -suvut. Tutkielma jakautuu neljään osaan: alkueläimet (Protozoa), laakamadot (Platyhelminthes), pyörömadot (Nemathelminthes) ja niveljalkaiset (Arthropoda).… (more)

Subjects/Keywords: loinen; Trypanosoma cervi; Giardia; Entamoeba; Eimeria; Isospora rangiferi; Sarcocystis; Besnoitia tarandi; Babesia divergens; poro; loiset; Patologinen anatomia; Patologisk anatomi; Pathological Anatomy; loinen; Trypanosoma cervi; Giardia; Entamoeba; Eimeria; Isospora rangiferi; Sarcocystis; Besnoitia tarandi; Babesia divergens

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Isomursu, M. (1996). Poron (Rangifer tarandus tarandus) loiset - kirjallisuuskatsaus. (Thesis). University of Helsinki. Retrieved from http://hdl.handle.net/1975/1156

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Isomursu, Marja. “Poron (Rangifer tarandus tarandus) loiset - kirjallisuuskatsaus.” 1996. Thesis, University of Helsinki. Accessed March 08, 2021. http://hdl.handle.net/1975/1156.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Isomursu, Marja. “Poron (Rangifer tarandus tarandus) loiset - kirjallisuuskatsaus.” 1996. Web. 08 Mar 2021.

Vancouver:

Isomursu M. Poron (Rangifer tarandus tarandus) loiset - kirjallisuuskatsaus. [Internet] [Thesis]. University of Helsinki; 1996. [cited 2021 Mar 08]. Available from: http://hdl.handle.net/1975/1156.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Isomursu M. Poron (Rangifer tarandus tarandus) loiset - kirjallisuuskatsaus. [Thesis]. University of Helsinki; 1996. Available from: http://hdl.handle.net/1975/1156

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Zambia

15. Konde, Victor. Comparative studies of the purified cysteine proteases of trypanosoma brucei and trypanosoma congolense .

Degree: 2011, University of Zambia

URL: http://hdl.handle.net/123456789/278

► The cysteine protease enzymes from T. congolense and T. brucei were purified from pure blood stream forms of the trypanosomes grown in adult albino rats.… (more)

▼ The cysteine protease enzymes from T. congolense and T. brucei were purified from pure blood stream forms of the trypanosomes grown in adult albino rats. The trypanosomes were harvested when the number of trypanosomes had reached 108/ml of infected blood or higher. The rats were supplied with 10% glucose in drinking water when the parasiteamia o was 10 /ml which enabled the rats to survive for at least two extra days. In case of T. brucei infections, the level of stumpy forms in blood was monitored and when the number of stumpy forms reached 50% or more the trypanosomes were harvested. This resulted in an increase in cysteine protease recovery by at least two fold. The enzymes were successfully purified using egg white cystatin immobilised on sepharose. There were no significant differences in the yields of the two cysteine proteases and both enzymes had similar activity. The activity of the two enzymes was assayed with carbobenzoxyphenylalaninyl- argininyl 7-amido-4-methylcoumarin by monitoring the fluorescence at 365 nm. The reactions were performed at pH 6 at room temperature (28 - 30°C). T. congolense cysteine protease was purified using a monoclonal antibody, prepared against the T. congolense cysteine protease, immobilised on sepharose. The enzyme was of similar molecular weight as that purified by the cystatin-sepharose column. The enzyme was incubated with a variety of host macromolecules, mainly connective tissue proteins and the digestion products were followed using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). hi other cases the proteins were copolymerized in SDS-PAGE and then digestion monitored in zymograms. The enzymes expressed proteolytic activity against collagen type I, collagen type IV, glycoproteins, fibronectin, laminin and to a small extent against elastin. Fibronectin was the best substrate of all the connective tissue proteins while elastin was least reactive. All the above connective tissue proteins were not denatured prior to digestion as reported in similar experiments. The two trypanosome cysteine proteases failed to round off and detach cells in culture at our working enzyme concentration (160 ug/ml). The enzymes had greater effect on the cell membrane than on the intercellular matrix, an effect that is similar to papain. The enzymes have high proteolytic activity against cell surface proteins and this may account for the failure to round off cells, as the enzymes are likely to preferentially attack cell surface proteins rather than proteoglycans in the extracellular matrix. The enzymes were inhibited by trans-epoxysuccinyl-1-leucylamido (4-guanidino) butane (E-64), egg white cystatin, and Noc-p-tosyl-1-l-lysine chloromethyl ketone (TLCK) while phenylmethylsulphonyl-fluoride (PMSF) and aprotinin failed to inhibit the enzymes to any noticeable extent. The enzymes digested bovine serum albumin (BSA), fibrinogen and casein and their proteolytic products were resolved on sodium dodecyl sulphate polyacrylamide gel…

Subjects/Keywords: : Cysteine proteinases; : Trypanosoma brucei

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Konde, V. (2011). Comparative studies of the purified cysteine proteases of trypanosoma brucei and trypanosoma congolense . (Thesis). University of Zambia. Retrieved from http://hdl.handle.net/123456789/278

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Konde, Victor. “Comparative studies of the purified cysteine proteases of trypanosoma brucei and trypanosoma congolense .” 2011. Thesis, University of Zambia. Accessed March 08, 2021. http://hdl.handle.net/123456789/278.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Konde, Victor. “Comparative studies of the purified cysteine proteases of trypanosoma brucei and trypanosoma congolense .” 2011. Web. 08 Mar 2021.

Vancouver:

Konde V. Comparative studies of the purified cysteine proteases of trypanosoma brucei and trypanosoma congolense . [Internet] [Thesis]. University of Zambia; 2011. [cited 2021 Mar 08]. Available from: http://hdl.handle.net/123456789/278.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Konde V. Comparative studies of the purified cysteine proteases of trypanosoma brucei and trypanosoma congolense . [Thesis]. University of Zambia; 2011. Available from: http://hdl.handle.net/123456789/278

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universiteit Utrecht

16. Willemse, B.R. Trypanosoma Evansi: An Experimental Infection In Mice.

Degree: 2007, Universiteit Utrecht

URL: http://dspace.library.uu.nl:8080/handle/1874/40135

► The experiments were aimed at creating sufficient infectious material to infect three groups of sow with Trypanosoma evansi and to evaluate whether there is a… (more)

Subjects/Keywords: Diergeneeskunde; trypanosoma, evansi, cryopreservation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Willemse, B. R. (2007). Trypanosoma Evansi: An Experimental Infection In Mice. (Doctoral Dissertation). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/40135

Chicago Manual of Style (16^th Edition):

Willemse, B R. “Trypanosoma Evansi: An Experimental Infection In Mice.” 2007. Doctoral Dissertation, Universiteit Utrecht. Accessed March 08, 2021. http://dspace.library.uu.nl:8080/handle/1874/40135.

MLA Handbook (7^th Edition):

Willemse, B R. “Trypanosoma Evansi: An Experimental Infection In Mice.” 2007. Web. 08 Mar 2021.

Vancouver:

Willemse BR. Trypanosoma Evansi: An Experimental Infection In Mice. [Internet] [Doctoral dissertation]. Universiteit Utrecht; 2007. [cited 2021 Mar 08]. Available from: http://dspace.library.uu.nl:8080/handle/1874/40135.

Council of Science Editors:

Willemse BR. Trypanosoma Evansi: An Experimental Infection In Mice. [Doctoral Dissertation]. Universiteit Utrecht; 2007. Available from: http://dspace.library.uu.nl:8080/handle/1874/40135

17. Rodrigues Silva, Alexandre. Cladonia verticillaris (Raddi) Fr. como agente tripanocida sobre Trypanosoma cruzi .

Degree: 2009, Universidade Federal de Pernambuco

URL: http://repositorio.ufpe.br/handle/123456789/665

► A doença de Chagas continua a ser um problema de Saúde Pública relevante e, o único tratamento, o Benznidazol, acarreta severos efeitos colaterais e possui… (more)

Subjects/Keywords: Líquen; Cladonia verticillaris; Trypanosoma cruzi

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Rodrigues Silva, A. (2009). Cladonia verticillaris (Raddi) Fr. como agente tripanocida sobre Trypanosoma cruzi . (Thesis). Universidade Federal de Pernambuco. Retrieved from http://repositorio.ufpe.br/handle/123456789/665

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Rodrigues Silva, Alexandre. “Cladonia verticillaris (Raddi) Fr. como agente tripanocida sobre Trypanosoma cruzi .” 2009. Thesis, Universidade Federal de Pernambuco. Accessed March 08, 2021. http://repositorio.ufpe.br/handle/123456789/665.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Rodrigues Silva, Alexandre. “Cladonia verticillaris (Raddi) Fr. como agente tripanocida sobre Trypanosoma cruzi .” 2009. Web. 08 Mar 2021.

Vancouver:

Rodrigues Silva A. Cladonia verticillaris (Raddi) Fr. como agente tripanocida sobre Trypanosoma cruzi . [Internet] [Thesis]. Universidade Federal de Pernambuco; 2009. [cited 2021 Mar 08]. Available from: http://repositorio.ufpe.br/handle/123456789/665.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Rodrigues Silva A. Cladonia verticillaris (Raddi) Fr. como agente tripanocida sobre Trypanosoma cruzi . [Thesis]. Universidade Federal de Pernambuco; 2009. Available from: http://repositorio.ufpe.br/handle/123456789/665

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

18. França Luis, Aracelly. Síntese, comprovação estrutural e avaliação da atividade anti-T cruzi de derivados imidazacrídinicos e imidazolidínicos .

Degree: 2008, Universidade Federal de Pernambuco

URL: http://repositorio.ufpe.br/handle/123456789/3351

► A doença de Chagas é uma infecção causada pelo Trypanosoma cruzi, o qual é transmitido ao homem através da picada de insetos pertencentes à espécie… (more)

Subjects/Keywords: Trypanosoma cruzi.; Imidazacridinas; Imidazolinas

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

França Luis, A. (2008). Síntese, comprovação estrutural e avaliação da atividade anti-T cruzi de derivados imidazacrídinicos e imidazolidínicos . (Thesis). Universidade Federal de Pernambuco. Retrieved from http://repositorio.ufpe.br/handle/123456789/3351

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

França Luis, Aracelly. “Síntese, comprovação estrutural e avaliação da atividade anti-T cruzi de derivados imidazacrídinicos e imidazolidínicos .” 2008. Thesis, Universidade Federal de Pernambuco. Accessed March 08, 2021. http://repositorio.ufpe.br/handle/123456789/3351.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

França Luis, Aracelly. “Síntese, comprovação estrutural e avaliação da atividade anti-T cruzi de derivados imidazacrídinicos e imidazolidínicos .” 2008. Web. 08 Mar 2021.

Vancouver:

França Luis A. Síntese, comprovação estrutural e avaliação da atividade anti-T cruzi de derivados imidazacrídinicos e imidazolidínicos . [Internet] [Thesis]. Universidade Federal de Pernambuco; 2008. [cited 2021 Mar 08]. Available from: http://repositorio.ufpe.br/handle/123456789/3351.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

França Luis A. Síntese, comprovação estrutural e avaliação da atividade anti-T cruzi de derivados imidazacrídinicos e imidazolidínicos . [Thesis]. Universidade Federal de Pernambuco; 2008. Available from: http://repositorio.ufpe.br/handle/123456789/3351

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

UCLA

19. Langousis, Gerasimos. Motility and sensory functions of the Trypanosoma brucei flagellum.

Degree: Microbiology, Immunology, & Molecular Genetics, 2014, UCLA

URL: http://www.escholarship.org/uc/item/8kd3r097

► Eukaryotic flagella, also known as cilia, are emblematic features of most extant eukaryotic cells. Cilia have important motility and sensory roles for cellular and organismal… (more)

▼ Eukaryotic flagella, also known as cilia, are emblematic features of most extant eukaryotic cells. Cilia have important motility and sensory roles for cellular and organismal physiology. A prime example is the flagellum of Trypanosoma brucei, the unicellular causative agent of sleeping sickness in Africa. The T.brucei flagellum harbors a canonical eukaryotic axoneme and provides cell propulsion, dictates cell morphogenesis as well as constitutes a critical host pathogen interface. Flagellar motility results from the coordinated activity of axonemal dyneins. While the mechanism, by which thousands of dynein motors are spatiotemporally controlled, is enigmatic, motility requires the Nexin Dynein Regulatory Complex (NDRC). The NDRC is a massive axonemal protein complex, present in virtually all flagellated eukaryotes, that reversibly inhibits dyneins. NDRC has important roles in human and trypanosome biology yet its exact composition and mode of action are unknown. We employed quantitative proteomics as well as a candidate approach to illuminate the T. brucei NDRC subunit composition. We discovered 14 proteins that are consistently reduced in axonemes of NDRC mutants and are thus prime NDRC subunit candidates. Of these candidates, only two were specific to the trypanosome lineage while the remainder had clear orthologs in diverse eukaryotes. Our results support the existence of an NDRC core consisting of phylogenetically conserved subunits that operate in unison with lineage specific subunits. Moreover, NDRC is likely a calcium-regulated hub given the extensive representation of cognate domains among its subunits.The trypanosome flagellum remains attached to the cell body for most of its length at the flagellum attachment zone (FAZ). FAZ has important structural and morphogenetic roles that dictate the shape and size of the trypanosome cell. Nevertheless, the protein repertoire of the FAZ has remained elusive. We focused on FS179, an uncharacterized membrane protein recently detected in our T.brucei flagellar membrane proteome. We showed that this putative calcium channel localizes to a subdomain of the flagellar membrane along the FAZ. Interestingly, FS179 mediates flagellum attachment and is required for cell shape and parasite viability. Our findings thus reveal a novel component of the trypanosome FAZ and shed light on flagellum roles in cell morphogenesis.The trypanosome flagellar membrane is the interface with the extracellular milieu and its proteins have vital roles in host-pathogen interactions. This is exemplified by the flagellar pocket, a membrane invagination at the base of the flagellum where all endocytosis and uptake of host factors takes place. During efforts to understand flagellar membrane functions, we studied BBS proteins, subunits of the BBSome complex that controls cilium localization for certain membrane proteins in other eukaryotes. We show that T.brucei BBS proteins assemble into a BBSome-like complex and localize to the flagellar pocket membrane and vesicles therein. Genetic knockouts of BBS…

Subjects/Keywords: Microbiology; flagellum; Trypanosoma brucei

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Langousis, G. (2014). Motility and sensory functions of the Trypanosoma brucei flagellum. (Thesis). UCLA. Retrieved from http://www.escholarship.org/uc/item/8kd3r097

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Langousis, Gerasimos. “Motility and sensory functions of the Trypanosoma brucei flagellum.” 2014. Thesis, UCLA. Accessed March 08, 2021. http://www.escholarship.org/uc/item/8kd3r097.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Langousis, Gerasimos. “Motility and sensory functions of the Trypanosoma brucei flagellum.” 2014. Web. 08 Mar 2021.

Vancouver:

Langousis G. Motility and sensory functions of the Trypanosoma brucei flagellum. [Internet] [Thesis]. UCLA; 2014. [cited 2021 Mar 08]. Available from: http://www.escholarship.org/uc/item/8kd3r097.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Langousis G. Motility and sensory functions of the Trypanosoma brucei flagellum. [Thesis]. UCLA; 2014. Available from: http://www.escholarship.org/uc/item/8kd3r097

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Alberta

20. Oladiran, Ayoola. Studies on the mechanisms of immune evasion in Trypanosoma carassii infections of the goldfish (Carassius auratus).

Degree: PhD, Department of Biological Sciences, 2012, University of Alberta

URL: https://era.library.ualberta.ca/files/3197xm80c

► Parasites possess variety of mechanisms to modulate or evade host defence systems to maintain chronic infection and ensure their transmission. The protozoan parasite Trypanosoma carassii… (more)

▼ Parasites possess variety of mechanisms to modulate or evade host defence systems to maintain chronic infection and ensure their transmission. The protozoan parasite Trypanosoma carassii is infective to a number of freshwater fish species and can cause significant mortality in aquaculture. T. carassii shares similarities with both T. brucei, the causative agent of sleeping sickness and T. cruzi, which causes Chagas’ disease. It is not known how T. carassii escapes host immune responses. My doctoral research focused on the interactions between T. carassii and its fish host with emphasis on the strategies used by the parasite to evade fish immune host defense.. and establish long lasting infections. I identified at the molecular level the key antigens of T. carassii present in excretory/secretory (ES) products and surface protein fractions of the parasite. As a first step toward understanding the importance of select antigens of T. carassii, I produced the recombinant proteins and characterized their roles in immune evasion. Like heat shock protein 70 of other pathogens, I found that T. carassii hsp70 was immunogenic and was present in both ES products and surface protein fraction of the parasite. Recombinant parasite hsp70 significantly increased expression of pro-inflammatory genes and enhanced inflammatory response of goldfish macrophages. In contrast, another parasite surface molecule, glycoprotein 63 (Gp63) down-regulated both pathogen and cytokine-induced inflammatory responses of goldfish monocytes and macrophages. Parasite gp63 was associated with macrophages and appeared to interfere with signalling mechanisms. Since complement-mediated lysis is one of the main host defence responses against trypanosomes, I cloned and characterized parasite surface molecule called calreticulin. Recombinant T. carassii calreticulin bound to first component of complement, C1q, of not only goldfish but also humans. Further, recombinant T. carassii calreticulin inhibited C1q-dependent hemolysis. T. carassii infection of goldfish induced increased expression of pro- and anti-inflammatory cytokines. Increased cytokine mRNA levels were observed during the acute phase of infection, and then they returned to normal levels or were down-regulated during the elimination phase of the infection. These findings demonstrate that parasite surface molecules and those found in ES fraction have the capacity to manipulate host inflammatory and antimicrobial responses, thereby ensuring persistence of T. carassii in its host.

Subjects/Keywords: Trypanosoma carassii; Goldfish; Immune evasion

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Oladiran, A. (2012). Studies on the mechanisms of immune evasion in Trypanosoma carassii infections of the goldfish (Carassius auratus). (Doctoral Dissertation). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/3197xm80c

Chicago Manual of Style (16^th Edition):

Oladiran, Ayoola. “Studies on the mechanisms of immune evasion in Trypanosoma carassii infections of the goldfish (Carassius auratus).” 2012. Doctoral Dissertation, University of Alberta. Accessed March 08, 2021. https://era.library.ualberta.ca/files/3197xm80c.

MLA Handbook (7^th Edition):

Oladiran, Ayoola. “Studies on the mechanisms of immune evasion in Trypanosoma carassii infections of the goldfish (Carassius auratus).” 2012. Web. 08 Mar 2021.

Vancouver:

Oladiran A. Studies on the mechanisms of immune evasion in Trypanosoma carassii infections of the goldfish (Carassius auratus). [Internet] [Doctoral dissertation]. University of Alberta; 2012. [cited 2021 Mar 08]. Available from: https://era.library.ualberta.ca/files/3197xm80c.

Council of Science Editors:

Oladiran A. Studies on the mechanisms of immune evasion in Trypanosoma carassii infections of the goldfish (Carassius auratus). [Doctoral Dissertation]. University of Alberta; 2012. Available from: https://era.library.ualberta.ca/files/3197xm80c

University of Manchester

21. Currin, Andrew. A study of protein phosphatases from the genomes of trypanosomatid parasites.

Degree: 2013, University of Manchester

URL: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:201557

►

Trypanosomiases and leishmaniases are amongst the world’s most neglected infectious diseases. Trypanosoma brucei, Trypanosoma cruzi and Leishmania major are the primary human pathogens of the… (more)

▼

Trypanosomiases and leishmaniases are amongst the world’s most neglected infectious diseases. Trypanosoma brucei, Trypanosoma cruzi and Leishmania major are the primary human pathogens of the trypanosomatidae family and the causative agents of African sleeping sickness, Chagas’ disease and cutaneous leishmaniasis, respectively. The molecular mechanism controlling each parasite’s life cycle and virulence is poorly understood however protein phosphatases are expected to play a critical role.This study presents the biochemical characterisation data of two groups of phosphatases from the trypanosomatids: the LRR-DSP and LM phosphatases.The LRR-DSPs are a group of twelve proteins, characterised by a unique domain architecture: a leucine-rich repeat (LRR) domain together with a dual- specificity phosphatase (DSP) catalytic domain. In this study, the recombinant expression of a representative LRR-DSP orthologue from T. brucei (TbLRR-DSP), proved to be highly problematic in E. coli. Most full-length and catalytic domain constructs were expressed at very low levels or as insoluble proteins. Soluble protein was obtained by denaturation, treatment with detergents, non-denaturing extraction from inclusion bodies and fusion to solubility-enhancing proteins. However, no method yielded protein with catalytic activity or detectable secondary structure. Soluble expression of TbLRR-DSP was achieved using baculovirus-infected insect cells, but the protein co-purified with endogenous chaperones and exhibited no catalytic activity thus implying a lack of correct folding.In the second part of this study, two phosphatases specific to Leishmania major, LM1 and LM2, were characterised and structural studies were initiated. LM2 was shown to readily hydrolyse phospho-tyrosine substrates in vitro, but not phosphoinositides like its homologue, LM1. Both proteins therefore have a differentiated catalytic profile and are likely to have different functions in vivo. Purification protocols for both proteins were established and crystallisation screenings set up. Preliminary hits were obtained for LM2 and a mutagenesis strategy was developed to improve chances of obtaining diffraction quality crystals. Recombinant LM1 samples exhibited heterogeneity and therefore will require additional engineering to improve chances of crystallization. Promising pilot NMR data was also obtained for both phosphatases.In conclusion, this study demonstrates that the recombinant expression of multi- domain trypanosomatid proteins (like the LRR-DSPs) can be highly problematic and may pose a challenge for their biochemical characterisation and functional elucidation. Future work into trypanosomatid phosphatases, however challenging, will improve our understanding of their cell biology and potentially identify therapeutic targets.

N/A

Advisors/Committee Members: TABERNERO, LYDIA L, Tabernero, Lydia, Bella, Jordi.

Subjects/Keywords: Phosphatase; trypanosoma; leishmania; trypanosomatid

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Currin, A. (2013). A study of protein phosphatases from the genomes of trypanosomatid parasites. (Doctoral Dissertation). University of Manchester. Retrieved from http://www.manchester.ac.uk/escholar/uk-ac-man-scw:201557

Chicago Manual of Style (16^th Edition):

Currin, Andrew. “A study of protein phosphatases from the genomes of trypanosomatid parasites.” 2013. Doctoral Dissertation, University of Manchester. Accessed March 08, 2021. http://www.manchester.ac.uk/escholar/uk-ac-man-scw:201557.

MLA Handbook (7^th Edition):

Currin, Andrew. “A study of protein phosphatases from the genomes of trypanosomatid parasites.” 2013. Web. 08 Mar 2021.

Vancouver:

Currin A. A study of protein phosphatases from the genomes of trypanosomatid parasites. [Internet] [Doctoral dissertation]. University of Manchester; 2013. [cited 2021 Mar 08]. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:201557.

Council of Science Editors:

Currin A. A study of protein phosphatases from the genomes of trypanosomatid parasites. [Doctoral Dissertation]. University of Manchester; 2013. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:201557

UNIVERSIDADE FEDERAL DE OURO PRETO

22. Sheler Martins Souza. Caracterização imuno-histoquímica do infiltrado inflamatório cardíaco durante a fase aguda da infecção experimental de cães por formas tripomastigotas metacíclicas ou sanguíneas da cepa Berenice-78 do Trypanosoma cruzi.

Degree: 2010, UNIVERSIDADE FEDERAL DE OURO PRETO

URL: http://www.tede.ufop.br/tde_busca/arquivo.php?codArquivo=671

► Nosso grupo vem estudando a infecção experimental de cães pelo Trypanosoma cruzi ao longo dos últimos 30 anos. Semelhante ao observado em humanos, no modelo… (more)

▼ Nosso grupo vem estudando a infecção experimental de cães pelo Trypanosoma cruzi ao longo dos últimos 30 anos. Semelhante ao observado em humanos, no modelo canino verifica-se um infiltrado inflamatório predominantemente constituído por células mononucleares durante a fase aguda da doença. A identificação destas células é uma estratégia importante para a compreensão dos mecanismos imunopatológicos desencadeados pelo parasito ao longo da infecção. Neste contexto, fez-se necessário a padronização de reações imuno-histoquímicas para caracterização do fenótipo das principais células presentes no infiltrado inflamatório (linfócitos T CD4 + e T CD8 + , macrófagos CD14 + e neutrófilos). As células envolvidas na infecção experimental aguda de cães por formas tripomastigotas metacíclicas ou sanguíneas da cepa Berenice-78 do T. cruzi foram quantificadas por análise morfométrica, no parênquima e no foco inflamatório, tanto no átrio direito quanto no septo interventricular. Observou-se que em ambos os fragmentos cardíacos, o processo inflamatório foi maior no grupo infectado por formas tripomastigotas sanguíneas. A quantificação do fenótipo celular mostrou que os linfócitos T CD4 + são predominantes no foco inflamatório nos animais infectados por ambas as formas, tanto no átrio direito quanto no septo interventricular. No átrio direito, os linfócitos T CD8 + foram observados em maior quantidade nos grupos infectados em relação ao grupo controle. Já os macrófagos CD14 + prevalecem apenas no grupo infectado por formas tripomastigotas metacíclicas em relação ao grupo controle. No septo intraventricular, os linfócitos T CD8 + e os macrófagos CD14 + encontram-se em elevado número no grupo infectado por formas tripomastigotas metacíclidas em relação ao grupo controle e ao grupo infectado por formas tripomastigotas sanguíneas. Os neutrófilos estão presentes em menor número em relação às demais células analisadas, mas são encontrados em maior número no átrio direito de ambos os grupos infectados. O quadro histopatológico mais preservado observado no grupo infectado por formas tripomastigota metacíclico pode estar diretamente relacionado à interação entre os linfócitos T CD8 + e os macrófagos CD 14 + , pois estas são potentes produtoras de interleucina-12, uma citocina que estimula a produção de interferon-gama pelos linfócitos T CD8 + . Esta citocina é fundamental para o processo de resistência à infecção pelo T. cruzi durante a fase aguda por estimular a síntese de óxido nítrico pelos macrófagos. A quantificação celular realizada de modo aleatório evidenciou melhor as diferenças entre os grupos. Contudo, a quantificação focal do processo inflamatório distinguiu melhor as porcentagens das populações e subpopulações celulares. Os resultados obtidos mostraram que as formas infectantes, tripomastigotas metacíclicas ou sanguíneas, apresentam perfil celular distinto no átrio direito e no septo intrerventricular, sendo a resposta elaborada durante a infecção com as formas tripomastigotas metacíclicas mais eficiente e menos lesiva ao… Advisors/Committee Members: Marcelo Vidigal Caliari, Ricardo Gonçalves, Claúdia Martins Carneiro.

Subjects/Keywords: Trypanosoma cruzi; IMUNOLOGIA CELULAR

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Souza, S. M. (2010). Caracterização imuno-histoquímica do infiltrado inflamatório cardíaco durante a fase aguda da infecção experimental de cães por formas tripomastigotas metacíclicas ou sanguíneas da cepa Berenice-78 do Trypanosoma cruzi. (Thesis). UNIVERSIDADE FEDERAL DE OURO PRETO. Retrieved from http://www.tede.ufop.br/tde_busca/arquivo.php?codArquivo=671

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Souza, Sheler Martins. “Caracterização imuno-histoquímica do infiltrado inflamatório cardíaco durante a fase aguda da infecção experimental de cães por formas tripomastigotas metacíclicas ou sanguíneas da cepa Berenice-78 do Trypanosoma cruzi.” 2010. Thesis, UNIVERSIDADE FEDERAL DE OURO PRETO. Accessed March 08, 2021. http://www.tede.ufop.br/tde_busca/arquivo.php?codArquivo=671.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Souza, Sheler Martins. “Caracterização imuno-histoquímica do infiltrado inflamatório cardíaco durante a fase aguda da infecção experimental de cães por formas tripomastigotas metacíclicas ou sanguíneas da cepa Berenice-78 do Trypanosoma cruzi.” 2010. Web. 08 Mar 2021.

Vancouver:

Souza SM. Caracterização imuno-histoquímica do infiltrado inflamatório cardíaco durante a fase aguda da infecção experimental de cães por formas tripomastigotas metacíclicas ou sanguíneas da cepa Berenice-78 do Trypanosoma cruzi. [Internet] [Thesis]. UNIVERSIDADE FEDERAL DE OURO PRETO; 2010. [cited 2021 Mar 08]. Available from: http://www.tede.ufop.br/tde_busca/arquivo.php?codArquivo=671.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Souza SM. Caracterização imuno-histoquímica do infiltrado inflamatório cardíaco durante a fase aguda da infecção experimental de cães por formas tripomastigotas metacíclicas ou sanguíneas da cepa Berenice-78 do Trypanosoma cruzi. [Thesis]. UNIVERSIDADE FEDERAL DE OURO PRETO; 2010. Available from: http://www.tede.ufop.br/tde_busca/arquivo.php?codArquivo=671

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Addis Ababa University

23. Hassen, Kebede. Assessment of trypanocidal Drug Resistant Trypanosoma congolense Isolates and Survey on Trypanocidal Drug Management Practices in Western Amhara Regional State, Ethiopia .

Degree: 2008, Addis Ababa University

URL: http://etd.aau.edu.et/dspace/handle/123456789/5686

► A study was conducted from December 2006 to July 2007 in trypanosomosis endemic areas of western Amhara with the objective of assessing trypanocidal drug resistant… (more)

▼ A study was conducted from December 2006 to July 2007 in trypanosomosis endemic areas of western Amhara with the objective of assessing trypanocidal drug resistant Trypanosoma congolense isolates and current status of trypanocidal drug management practices.Experimental trypanocidal drug sensitivity test was performed in experimentally infected mice using combined and separately pooled trypanosomal isolates obtained from different districts. This was complimented by a survey that targeted farmers and health professionals involved in veterinary service aiming at current status of trypanocidal drugs. Questionniary survey on farmers revealed that 71.7 % of the respondents had the experience of using trypanocidal drugs by themselves and at least 54 % of them practiced underdosing with any of the three trypanocidal drugs (diminazene aceturate, isometamidium chloride and homidium bromide). Furthermore, from questionnaire survey on professionals, 55.1% of the respondents in veterinary service (MoARD) indicated the use of increased dosages as a measure to avert treatement failures. Point prevalence of trypanosomosis obtained from cattle screened for isolation was found to be 12.4% in Debreelias, 20% in Dembecha and 27.2% for Jabitahnan. T. congolense accounted for 72.9% the overall trypanosomosis cases. The drugs tested failed to permanently clear the parasite, being relapsing at 5.8±0.96 days with 70 mg/kg diminazine aceturate; 14.2±1.99 days with 10 mg/kg isometamidium chloride and 4.6±3.75 days with 10 mg/kg homidium bromide. The difference in the relapse duration was found to be statistically significant among drugs, being longer with isometamidium (p<0.001) than others. The drugs were also unable to permanently clear trypanosomes from the combined pool when given in combination at intervals, as there was a 40 % relapse with 70 mg/kg diminazene aceturate initial treatement followed by isometamidium 5 mg/kg at 17±3.92days. However sanative pairs appeared to be relatively effective than single drug alone, as relapse duration was significantly longer (P<0.001) in the former than the latter. Separate pools from each district was also used to compare the relative contribution of drug resistance phenotype in combined pool and similar results were observed (relapses at 7.8±1.57 days; 7.0±1.24 days and 5.8±0.96 days with 70 mg/kg diminazene aceturate; 17.50±1.88 days; 18.20±2.66 days and 11.00±2.15 days with 10 mg/kg isometamidium chloride in pools from Debreelias, Dembecha and Jabitahnan, respectively). The present study revelaed the presence of multidrug resistance that could most probably be attributed to the drug-use practices excercised the livestock production system. Wise use of currently available trypanocidals in conjunction with other alternative control strategies should be adopted as timely solution for the current problem of trypanosomosis in the region. Advisors/Committee Members: Ephrem Engidawork (PhD) (advisor).

Subjects/Keywords: Bovine; Relapse Duration; Trypanosoma congolense

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hassen, K. (2008). Assessment of trypanocidal Drug Resistant Trypanosoma congolense Isolates and Survey on Trypanocidal Drug Management Practices in Western Amhara Regional State, Ethiopia . (Thesis). Addis Ababa University. Retrieved from http://etd.aau.edu.et/dspace/handle/123456789/5686

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Hassen, Kebede. “Assessment of trypanocidal Drug Resistant Trypanosoma congolense Isolates and Survey on Trypanocidal Drug Management Practices in Western Amhara Regional State, Ethiopia .” 2008. Thesis, Addis Ababa University. Accessed March 08, 2021. http://etd.aau.edu.et/dspace/handle/123456789/5686.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Hassen, Kebede. “Assessment of trypanocidal Drug Resistant Trypanosoma congolense Isolates and Survey on Trypanocidal Drug Management Practices in Western Amhara Regional State, Ethiopia .” 2008. Web. 08 Mar 2021.

Vancouver:

Hassen K. Assessment of trypanocidal Drug Resistant Trypanosoma congolense Isolates and Survey on Trypanocidal Drug Management Practices in Western Amhara Regional State, Ethiopia . [Internet] [Thesis]. Addis Ababa University; 2008. [cited 2021 Mar 08]. Available from: http://etd.aau.edu.et/dspace/handle/123456789/5686.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hassen K. Assessment of trypanocidal Drug Resistant Trypanosoma congolense Isolates and Survey on Trypanocidal Drug Management Practices in Western Amhara Regional State, Ethiopia . [Thesis]. Addis Ababa University; 2008. Available from: http://etd.aau.edu.et/dspace/handle/123456789/5686

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

24. SANDES, Jana Messias. Caracterização molecular e ultraestrutural do estresse do retículo endoplasmático de Trypanosoma cruzi .

Degree: 2016, Universidade Federal de Pernambuco

URL: https://repositorio.ufpe.br/handle/123456789/17356

► O retículo endoplasmático (RE) é uma organela vital para as células eucarióticas, envolvido na síntese, modificação e enovelamento de proteínas, homeostase do cálcio e metabolismo… (more)

▼ O retículo endoplasmático (RE) é uma organela vital para as células eucarióticas, envolvido na síntese, modificação e enovelamento de proteínas, homeostase do cálcio e metabolismo de lipídios. Variações no nível de cálcio, inibição da glicosilação, estresse oxidativo, entre outros, podem levar o RE a uma condição de estresse, a qual dispara vias de sinalização específicas incluindo a Unfolded Protein Response (UPR). A UPR promove aumento na expressão de chaperonas, inibe a tradução de novas proteínas e aumenta a degradação de proteínas mal enoveladas. No entanto, quando o estresse é severo ou persistente, a UPR induz mecanismos de morte celular, principalmente por apoptose. Embora o RE possua papel fundamental na sobrevivência das células de mamíferos, estudos sobre a fisiologia desta organela em Trypanosoma cruzi, agente etiológico da Doença de Chagas, ainda são escassos. Dessa forma, o objetivo deste trabalho foi caracterizar molecular e morfologicamente a resposta de formas epimastigotas de T. cruzi ao estresse do RE induzido por ditiotreitol (DTT) ou tunicamicina (TM). As células tratadas com DTT apresentaram inibição do crescimento de maneira dose-dependente, com recuperação do crescimento após a retirada da droga. O tratamento com DTT não alterou os níveis proteicos de BiP (binding protein) mas reduziu significativamente os níveis de RNAm de BiP e calreticulina (CRT), sugerindo que o T. cruzi tenha uma resposta diferente da UPR de eucariotos superiores. O estresse persistente do RE induzido pelo DTT causou drásticas alterações morfológicas e fisiológicas compatíveis com morte celular por apoptose tardia/necrose, como observado pela dupla marcação com anexina-V e iodeto de propídeo, além da redução do tamanho do corpo celular, inchaço e desorganização das cristas mitocondriais, despolarização do potencial de membrana mitocondrial (ψm) e aumento da produção (21 – 94%) de espécies reativas de oxigênio (ROS). No entanto, a presença de perfis do RE envolvendo porções do citoplasma sugere que a autofagia também esteja ocorrendo. Por outro lado, a TM apresentou um forte efeito tripanostático, sem crescimento celular independentemente da dose ou do tempo de incubação, inclusive após a retirada da droga. O tratamento com a TM também não foi capaz de alterar os níveis proteicos de BiP, porém induziu um aumento nos níveis de RNAm de BiP e CRT. A análise por citometria de fluxo demonstrou que o tratamento com TM induziu despolarização do ψm sem um aumento pronunciado na produção de ROS, e apenas cerca de 10% das células tratadas apresentaram fenótipos de apoptose. Alterações ultraestruturais também foram observadas nas células tratadas com TM, como o arredondamento do corpo celular, a presença de inclusões lipídicas e um grande número de perfis de RE ao redor de estruturas citoplasmáticas em processo de degradação. Dessa forma, nossos resultados sugerem que o tratamento com DTT compromete o funcionamento da célula de maneira geral mais do que atua como estressor do RE, devido ao seu forte efeito oxidativo, levando à… Advisors/Committee Members: FONTES, Adriana (advisor), FIGUEIREDO, Regina C. B. Q (advisor).

Subjects/Keywords: Protozoário; Trypanosoma cruzi; Citologia

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

SANDES, J. M. (2016). Caracterização molecular e ultraestrutural do estresse do retículo endoplasmático de Trypanosoma cruzi . (Thesis). Universidade Federal de Pernambuco. Retrieved from https://repositorio.ufpe.br/handle/123456789/17356

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

SANDES, Jana Messias. “Caracterização molecular e ultraestrutural do estresse do retículo endoplasmático de Trypanosoma cruzi .” 2016. Thesis, Universidade Federal de Pernambuco. Accessed March 08, 2021. https://repositorio.ufpe.br/handle/123456789/17356.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

SANDES, Jana Messias. “Caracterização molecular e ultraestrutural do estresse do retículo endoplasmático de Trypanosoma cruzi .” 2016. Web. 08 Mar 2021.

Vancouver:

SANDES JM. Caracterização molecular e ultraestrutural do estresse do retículo endoplasmático de Trypanosoma cruzi . [Internet] [Thesis]. Universidade Federal de Pernambuco; 2016. [cited 2021 Mar 08]. Available from: https://repositorio.ufpe.br/handle/123456789/17356.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

SANDES JM. Caracterização molecular e ultraestrutural do estresse do retículo endoplasmático de Trypanosoma cruzi . [Thesis]. Universidade Federal de Pernambuco; 2016. Available from: https://repositorio.ufpe.br/handle/123456789/17356

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Ghana

25. Amisigo, C.M. Effects of Iron Chelators on Bloodstream Forms of Trypanosoma Brucei .

Degree: 2018, University of Ghana

URL: http://ugspace.ug.edu.gh/handle/123456789/27660

► African trypanosomiasis still remains a lethal disease to both human and livestock. The disease persists due to limited drug availability, toxicity and emergence of drug… (more)

Subjects/Keywords: Iron Chelators; Trypanosoma Brucei

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Amisigo, C. M. (2018). Effects of Iron Chelators on Bloodstream Forms of Trypanosoma Brucei . (Masters Thesis). University of Ghana. Retrieved from http://ugspace.ug.edu.gh/handle/123456789/27660

Chicago Manual of Style (16^th Edition):

Amisigo, C M. “Effects of Iron Chelators on Bloodstream Forms of Trypanosoma Brucei .” 2018. Masters Thesis, University of Ghana. Accessed March 08, 2021. http://ugspace.ug.edu.gh/handle/123456789/27660.

MLA Handbook (7^th Edition):

Amisigo, C M. “Effects of Iron Chelators on Bloodstream Forms of Trypanosoma Brucei .” 2018. Web. 08 Mar 2021.

Vancouver:

Amisigo CM. Effects of Iron Chelators on Bloodstream Forms of Trypanosoma Brucei . [Internet] [Masters thesis]. University of Ghana; 2018. [cited 2021 Mar 08]. Available from: http://ugspace.ug.edu.gh/handle/123456789/27660.

Council of Science Editors:

Amisigo CM. Effects of Iron Chelators on Bloodstream Forms of Trypanosoma Brucei . [Masters Thesis]. University of Ghana; 2018. Available from: http://ugspace.ug.edu.gh/handle/123456789/27660

Universidad de Chile

26. Cofré Lorca, Laura Alicia. Generación de la proteína de fusión N-cMyc-TcAP1 como herramienta para la futura identificación de proteínas que interaccionan con la endonucleasa apurínica/apirimidínica TcAP1 de Trypanosoma cruzi.

Degree: 2017, Universidad de Chile

URL: http://repositorio.uchile.cl/handle/2250/170140

► El agente etiológico de la enfermedad de Chagas es el parásito hemoflagelado Trypanosoma cruzi (T. cruzi). Esta enfermedad posee un carácter endémico en América Latina… (more)

▼ El agente etiológico de la enfermedad de Chagas es el parásito hemoflagelado Trypanosoma cruzi (T. cruzi). Esta enfermedad posee un carácter endémico en América Latina y se estima un promedio de 8 millones de personas infectadas. El ciclo de vida de T. cruzi involucra insectos vectores y mamíferos hospederos, en los cuales se diferencia en tres formas celulares finales: epimastigote (extracelular y replicativa) presente en los vectores triatominos; tripomastigote (extracelular no replicativa e infectiva) que se encuentra tanto en insectos vectores como en hospederos mamíferos; y amastigote (intracelular y replicativa) presente en hospederos mamíferos. En ambos hospederos, T. cruzi se encuentra sometido a la acción de especies reactivas de oxígeno y nitrógeno (ROS/RNS) que dañan su DNA. No obstante, el parásito sobrevive a la acción del estrés oxidativo. En la mayoría de los eucariontes, el daño oxidativo al DNA es reparado principalmente por la vía de reparación por escisión de bases (BER), proceso conservado en el que participan una serie de proteínas con actividad enzimática, siendo fundamentales las endonucleasas apurínicas/apirimidínicas (endonucleasas AP). En el genoma de T. cruzi se identificó la secuencia de la endonucleasa AP TcAP1, homóloga al endonucleasa AP de humano (APE1). Se demostró que la sobrexpresión de esta enzima incrementa la viabilidad de epimastigotes y tripomastigotes expuestos a ROS/RNS y que la inhibición de la actividad de TcAP1 genera el efecto opuesto; es decir, disminuye la viabilidad de los parásitos tratados con agentes oxidantes. Actualmente, se sabe que APE1 humana se relaciona directamente con otras enzimas de la vía BER y modula la expresión de proteínas que participan de otras vías de reparación del DNA. Hasta el momento no se ha determinado si TcAP1 se asocia a otras proteínas, como ocurre con su ortóloga APE1 en humanos. En esta memoria de título se propuso generar herramientas que permitan a futuro identificar las proteínas parasitarias asociadas a la endonucleasa TcAP1 de T. cruzi, las cuales podrían modular o ser moduladas por ella. Para tales efectos, se diseñaron dos construcciones plasmidiales (pTREX-(ha)3-(flag)3-tcap1 y pTREX-cmyc-tcap1) con el objetivo de generar proteínas de fusión de TcAP1 asociadas a diferentes epítopes ((HA)3-(FLAG)3 o c-Myc). Estos vectores de expresión se transfectaron en XIepimastigotes de la cepa Y de T. cruzi, los que se seleccionaron con el antibiótico G-418. Solo se obtuvieron epimastigotes transfectantes que expresaron la proteína de fusión N-cMyc-TcAP1, evidenciado mediante ensayos de Western blot. Utilizando un kit comercial (anti-cMyc), se realizaron ensayos de inmunoprecipitación sobre homogeneizados de proteínas totales obtenidas desde los epimastigotes transfectantes que expresan N-cMyc-TcAP1 y epimastigotes control no transfectados. Las proteínas obtenidas se separaron mediante electroforesis bidimensional en condiciones desnaturantes con el objetivo de evidenciar patrones electroforéticos diferenciales entre parásitos transfectantes…

Subjects/Keywords: Trypanosoma cruzi; Endonucleasas; Proteínas

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Cofré Lorca, L. A. (2017). Generación de la proteína de fusión N-cMyc-TcAP1 como herramienta para la futura identificación de proteínas que interaccionan con la endonucleasa apurínica/apirimidínica TcAP1 de Trypanosoma cruzi. (Thesis). Universidad de Chile. Retrieved from http://repositorio.uchile.cl/handle/2250/170140

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Cofré Lorca, Laura Alicia. “Generación de la proteína de fusión N-cMyc-TcAP1 como herramienta para la futura identificación de proteínas que interaccionan con la endonucleasa apurínica/apirimidínica TcAP1 de Trypanosoma cruzi.” 2017. Thesis, Universidad de Chile. Accessed March 08, 2021. http://repositorio.uchile.cl/handle/2250/170140.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Cofré Lorca, Laura Alicia. “Generación de la proteína de fusión N-cMyc-TcAP1 como herramienta para la futura identificación de proteínas que interaccionan con la endonucleasa apurínica/apirimidínica TcAP1 de Trypanosoma cruzi.” 2017. Web. 08 Mar 2021.

Vancouver:

Cofré Lorca LA. Generación de la proteína de fusión N-cMyc-TcAP1 como herramienta para la futura identificación de proteínas que interaccionan con la endonucleasa apurínica/apirimidínica TcAP1 de Trypanosoma cruzi. [Internet] [Thesis]. Universidad de Chile; 2017. [cited 2021 Mar 08]. Available from: http://repositorio.uchile.cl/handle/2250/170140.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Cofré Lorca LA. Generación de la proteína de fusión N-cMyc-TcAP1 como herramienta para la futura identificación de proteínas que interaccionan con la endonucleasa apurínica/apirimidínica TcAP1 de Trypanosoma cruzi. [Thesis]. Universidad de Chile; 2017. Available from: http://repositorio.uchile.cl/handle/2250/170140

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

27. RODRIGUES, Felicidade Caroline. Prospecção fitoquímica e bioatividade de Sida galheirensis ULBR. (Malvaceae) .

Degree: 2020, Universidade Federal de Pernambuco

URL: https://repositorio.ufpe.br/handle/123456789/37616

► O gênero Sida demonstra ser um gênero promissor na descoberta de novas substâncias que possam combater a resistência microbiana e parasitária, contando com várias espécies… (more)

▼ O gênero Sida demonstra ser um gênero promissor na descoberta de novas substâncias que possam combater a resistência microbiana e parasitária, contando com várias espécies em que esse potencial foi elucidado. A hipótese geral do trabalho é que a Sida galheirensis Ulbr., espécie endêmica do Nordeste brasileiro, tenha potencial bioativo devido à quimiotaxonomia do gênero. O primeiro capítulo traz uma atualização das atividades biológicas, farmacológicas, etnomedicinais e da fitoquímica do gênero Sida. A pesquisa foi realizada nas principais plataformas num intervalo temporal de 2015 a 2019. A importância de Sida e 121 substâncias foram descritas nos últimos 05 anos, destas 89 foram identificadas no gênero pela primeira vez. Terpenóides e ácidos graxos foram as classes mais abundantes com maior número de substâncias encontradas. O segundo capítulo avaliou a composição química e a bioatividade de Sida galheirensis. A identificação dos compostos químicos foi realizada através de um UPLC-QTOF-MS/MS no modo iônico negativo. A atividade antimicrobiana foi determinada pelo método de microdiluição em caldo e a Concentração Inibitória Mínima (CIM) para bactérias e a IC50 (Concentração Inibitória de 50% dos Microrganismos) para fungos foi verificada. O efeito combinado dos produtos foi analisado em concentrações sub-inibitórias. O potencial antiparasitário foi avaliado contra as formas promastigotas de Leishmania spp. e epimastigotas de Trypanossoma cruzi. A caracterização química identificou 06 substâncias no extrato etanólico (02 flavonóis, 01 antocianidina e 03 ácidos graxos) e 03 no extrato acetato de etila (01 antocianidina e 02 ácidos graxos). A atividade antimicrobiana foi considerada irrelevante para fungos e bactérias quando os extratos foram testados isoladamente, porém o potencial modulador foi significativo contra as cepas bacterianas de Staphylococcus aureus quando associados a ofloxacina. Os extratos modularam significativamente o antifúngico fluconazol contra as cepas Candida. Para Candida albicans (INCQS 40006) o extrato etanólico e acetato de etila tiveram IC50 de 35,79 e 54,66 μg/mL, respectivamente, para C. albicans (URM 5974) a IC50 foi de 16,61 e 22,42 μg/mL. O extrato acetato também potencializou o efeito do fluconazol contra Candida tropicalis (URM 4262) (IC50 = 63,41 μg/mL). Os extratos apresentaram resultados significativos contra T. cruzi com IC50 de 341,3 e 227 μg/mL e, nenhum resultado contra Leishmania em concentrações clinicamente relevantes. Os resultados indicam que Sida é um gênero promissor no desenvolvimento de novos agentes para o combate a resistência microbiana e parasitária e, que S. galheirensis é uma das espécies com potencial bioativo corroborando a nossa hipótese inicial. Advisors/Committee Members: OLIVEIRA, Antônio Fernando Morais de (advisor), BRAGA, Maria Flaviana Bezerra Morais (advisor), http://lattes.cnpq.br/4699945697916110 (advisor), http://lattes.cnpq.br/1557613482101211 (advisor).

Subjects/Keywords: Candida; Staphylococcus aureus; Trypanosoma cruzi

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

RODRIGUES, F. C. (2020). Prospecção fitoquímica e bioatividade de Sida galheirensis ULBR. (Malvaceae) . (Masters Thesis). Universidade Federal de Pernambuco. Retrieved from https://repositorio.ufpe.br/handle/123456789/37616

Chicago Manual of Style (16^th Edition):

RODRIGUES, Felicidade Caroline. “Prospecção fitoquímica e bioatividade de Sida galheirensis ULBR. (Malvaceae) .” 2020. Masters Thesis, Universidade Federal de Pernambuco. Accessed March 08, 2021. https://repositorio.ufpe.br/handle/123456789/37616.

MLA Handbook (7^th Edition):

RODRIGUES, Felicidade Caroline. “Prospecção fitoquímica e bioatividade de Sida galheirensis ULBR. (Malvaceae) .” 2020. Web. 08 Mar 2021.

Vancouver:

RODRIGUES FC. Prospecção fitoquímica e bioatividade de Sida galheirensis ULBR. (Malvaceae) . [Internet] [Masters thesis]. Universidade Federal de Pernambuco; 2020. [cited 2021 Mar 08]. Available from: https://repositorio.ufpe.br/handle/123456789/37616.

Council of Science Editors:

RODRIGUES FC. Prospecção fitoquímica e bioatividade de Sida galheirensis ULBR. (Malvaceae) . [Masters Thesis]. Universidade Federal de Pernambuco; 2020. Available from: https://repositorio.ufpe.br/handle/123456789/37616

University of Manchester

28. Currin, Andrew. A study of protein phosphatases from the genomes of trypanosomatid parasites.

Degree: PhD, 2013, University of Manchester

URL: https://www.research.manchester.ac.uk/portal/en/theses/a-study-of-protein-phosphatases-from-the-genomes-of-trypanosomatid-parasites(6afd7092-b7e6-4816-903b-647d77942e95).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.617952

► Trypanosomiases and leishmaniases are amongst the world’s most neglected infectious diseases. Trypanosoma brucei, Trypanosoma cruzi and Leishmania major are the primary human pathogens of the… (more)

▼ Trypanosomiases and leishmaniases are amongst the world’s most neglected infectious diseases. Trypanosoma brucei, Trypanosoma cruzi and Leishmania major are the primary human pathogens of the trypanosomatidae family and the causative agents of African sleeping sickness, Chagas’ disease and cutaneous leishmaniasis, respectively. The molecular mechanism controlling each parasite’s life cycle and virulence is poorly understood however protein phosphatases are expected to play a critical role. This study presents the biochemical characterisation data of two groups of phosphatases from the trypanosomatids: the LRR-DSP and LM phosphatases. The LRR-DSPs are a group of twelve proteins, characterised by a unique domain architecture: a leucine-rich repeat (LRR) domain together with a dual- specificity phosphatase (DSP) catalytic domain. In this study, the recombinant expression of a representative LRR-DSP orthologue from T. brucei (TbLRR-DSP), proved to be highly problematic in E. coli. Most full-length and catalytic domain constructs were expressed at very low levels or as insoluble proteins. Soluble protein was obtained by denaturation, treatment with detergents, non-denaturing extraction from inclusion bodies and fusion to solubility-enhancing proteins. However, no method yielded protein with catalytic activity or detectable secondary structure. Soluble expression of TbLRR-DSP was achieved using baculovirus-infected insect cells, but the protein co-purified with endogenous chaperones and exhibited no catalytic activity thus implying a lack of correct folding. In the second part of this study, two phosphatases specific to Leishmania major, LM1 and LM2, were characterised and structural studies were initiated. LM2 was shown to readily hydrolyse phospho-tyrosine substrates in vitro, but not phosphoinositides like its homologue, LM1. Both proteins therefore have a differentiated catalytic profile and are likely to have different functions in vivo. Purification protocols for both proteins were established and crystallisation screenings set up. Preliminary hits were obtained for LM2 and a mutagenesis strategy was developed to improve chances of obtaining diffraction quality crystals. Recombinant LM1 samples exhibited heterogeneity and therefore will require additional engineering to improve chances of crystallization. Promising pilot NMR data was also obtained for both phosphatases. In conclusion, this study demonstrates that the recombinant expression of multi- domain trypanosomatid proteins (like the LRR-DSPs) can be highly problematic and may pose a challenge for their biochemical characterisation and functional elucidation. Future work into trypanosomatid phosphatases, however challenging, will improve our understanding of their cell biology and potentially identify therapeutic targets.

Subjects/Keywords: 616.9; Phosphatase; trypanosoma; leishmania; trypanosomatid

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Currin, A. (2013). A study of protein phosphatases from the genomes of trypanosomatid parasites. (Doctoral Dissertation). University of Manchester. Retrieved from https://www.research.manchester.ac.uk/portal/en/theses/a-study-of-protein-phosphatases-from-the-genomes-of-trypanosomatid-parasites(6afd7092-b7e6-4816-903b-647d77942e95).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.617952

Chicago Manual of Style (16^th Edition):

Currin, Andrew. “A study of protein phosphatases from the genomes of trypanosomatid parasites.” 2013. Doctoral Dissertation, University of Manchester. Accessed March 08, 2021. https://www.research.manchester.ac.uk/portal/en/theses/a-study-of-protein-phosphatases-from-the-genomes-of-trypanosomatid-parasites(6afd7092-b7e6-4816-903b-647d77942e95).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.617952.

MLA Handbook (7^th Edition):

Currin, Andrew. “A study of protein phosphatases from the genomes of trypanosomatid parasites.” 2013. Web. 08 Mar 2021.

Vancouver:

Currin A. A study of protein phosphatases from the genomes of trypanosomatid parasites. [Internet] [Doctoral dissertation]. University of Manchester; 2013. [cited 2021 Mar 08]. Available from: https://www.research.manchester.ac.uk/portal/en/theses/a-study-of-protein-phosphatases-from-the-genomes-of-trypanosomatid-parasites(6afd7092-b7e6-4816-903b-647d77942e95).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.617952.

Council of Science Editors:

Currin A. A study of protein phosphatases from the genomes of trypanosomatid parasites. [Doctoral Dissertation]. University of Manchester; 2013. Available from: https://www.research.manchester.ac.uk/portal/en/theses/a-study-of-protein-phosphatases-from-the-genomes-of-trypanosomatid-parasites(6afd7092-b7e6-4816-903b-647d77942e95).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.617952

University of Dundee

29. Bandini, Giulia. Studies on fucosylation in Trypanosoma brucei.

Degree: PhD, 2011, University of Dundee

URL: https://discovery.dundee.ac.uk/en/studentTheses/c74554c1-f4d3-4bb3-aa31-899fcf507e11 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.578809

► The biosynthesis of GDP-Fucose, the activated donor for fucose, has been recently shown to be essential in the parasite Trypanosoma brucei. Fucose is a common… (more)

Subjects/Keywords: 572.56; Trypanosoma brucei; Fucose; Glycobiology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bandini, G. (2011). Studies on fucosylation in Trypanosoma brucei. (Doctoral Dissertation). University of Dundee. Retrieved from https://discovery.dundee.ac.uk/en/studentTheses/c74554c1-f4d3-4bb3-aa31-899fcf507e11 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.578809

Chicago Manual of Style (16^th Edition):

Bandini, Giulia. “Studies on fucosylation in Trypanosoma brucei.” 2011. Doctoral Dissertation, University of Dundee. Accessed March 08, 2021. https://discovery.dundee.ac.uk/en/studentTheses/c74554c1-f4d3-4bb3-aa31-899fcf507e11 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.578809.

MLA Handbook (7^th Edition):

Bandini, Giulia. “Studies on fucosylation in Trypanosoma brucei.” 2011. Web. 08 Mar 2021.

Vancouver:

Bandini G. Studies on fucosylation in Trypanosoma brucei. [Internet] [Doctoral dissertation]. University of Dundee; 2011. [cited 2021 Mar 08]. Available from: https://discovery.dundee.ac.uk/en/studentTheses/c74554c1-f4d3-4bb3-aa31-899fcf507e11 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.578809.

Council of Science Editors:

Bandini G. Studies on fucosylation in Trypanosoma brucei. [Doctoral Dissertation]. University of Dundee; 2011. Available from: https://discovery.dundee.ac.uk/en/studentTheses/c74554c1-f4d3-4bb3-aa31-899fcf507e11 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.578809

30. Mookherjee, Neeloffer. Membrane glycoconjugates of procyclic Trypanosoma simiae and Trypanosoma congolense, members of the subgenus Nannomonas are immunologically similar and biochemically distinct.

Degree: Department of Biochemistry and Microbiology, 2018, University of Victoria

URL: https://dspace.library.uvic.ca//handle/1828/10212

► The surface molecules (procyclins) of procyclic forms of African trypanosomes (Trypanosoma brucei spp.) are complex mixtures of lipid-anchored glycoconjugates. The procyclins are expressed differentially during… (more)

▼ The surface molecules (procyclins) of procyclic forms of African trypanosomes (Trypanosoma brucei spp.) are complex mixtures of lipid-anchored glycoconjugates. The procyclins are expressed differentially during the parasite life cycle within the tsetse fly vector. It has been hypothesised that these surface molecules are involved in interactions with molecules of the tsetse fly and may influence differentiation, cell death and tissue tropism. To understand procyclin functions it is necessary to identify and characterise them. This thesis presents a study of the biochemical and immunochemical characteristics of the major surface molecules of Trypanosoma simiae and Trypanosoma congolense, animal pathogens of the subgenus Nannomonas that share the same developmental cycle and tropism within the tsetse vector. Organic solvent extraction, reverse-phase high performance liquid chromatography and enzyme-linked imnunosorbent assay using surface binding monoclonal antibodies were used to isolate membrane molecules of procyclic culture forms (PCF) of both trypanosome species. Gel electrophoresis of the purified molecules revealed two predominant molecular species from each parasite that were broadly similar yet showed different apparent molecular masses and staining characteristics. The molecules were shown to be glycosylphosphatidylinositol-lipid anchored glycoconjugates, comprised mainly of carbohydrates. Each moiety displayed surface-disposed carbohydrate epitopes that were recognised on the surface of both species of trypanosomes by monoclonal antibodies specific for procyclic parasites of the subgenus Nannomonas. The epitopes were previously shown to be displayed on the glutamic acid-alanine rich protein (GARP) of T. congolense, yet neither this protein (as detected either immunologically or by mass spectrometry) nor its encoding gene (as detected by Southern blot analysis) was present in T. simiae. The results indicate that although T. congolense and T. simiae share common carbohydrate surface epitopes, these are displayed on biochemically different molecules. I hypothesise that the surface disposed carbohydrate structures and not the polypeptide moieties are involved in parasite-tsetse interactions since these species have the same developmental cycles in the insect vector. In an attempt to obtain primary sequence information for the T. simiae PCF surface molecules, I identified and characterised an unique open reading frame. This was shown to be expressed as a protein in PCF and is likely a membrane-associated molecule of the subgenus Nannomonas. Advisors/Committee Members: Pearson, Terry W. (supervisor).

Subjects/Keywords: Trypanosoma; Immunogenetics; Tsetse-flies; Glycoconjugates

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mookherjee, N. (2018). Membrane glycoconjugates of procyclic Trypanosoma simiae and Trypanosoma congolense, members of the subgenus Nannomonas are immunologically similar and biochemically distinct. (Thesis). University of Victoria. Retrieved from https://dspace.library.uvic.ca//handle/1828/10212

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mookherjee, Neeloffer. “Membrane glycoconjugates of procyclic Trypanosoma simiae and Trypanosoma congolense, members of the subgenus Nannomonas are immunologically similar and biochemically distinct.” 2018. Thesis, University of Victoria. Accessed March 08, 2021. https://dspace.library.uvic.ca//handle/1828/10212.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mookherjee, Neeloffer. “Membrane glycoconjugates of procyclic Trypanosoma simiae and Trypanosoma congolense, members of the subgenus Nannomonas are immunologically similar and biochemically distinct.” 2018. Web. 08 Mar 2021.

Vancouver:

Mookherjee N. Membrane glycoconjugates of procyclic Trypanosoma simiae and Trypanosoma congolense, members of the subgenus Nannomonas are immunologically similar and biochemically distinct. [Internet] [Thesis]. University of Victoria; 2018. [cited 2021 Mar 08]. Available from: https://dspace.library.uvic.ca//handle/1828/10212.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mookherjee N. Membrane glycoconjugates of procyclic Trypanosoma simiae and Trypanosoma congolense, members of the subgenus Nannomonas are immunologically similar and biochemically distinct. [Thesis]. University of Victoria; 2018. Available from: https://dspace.library.uvic.ca//handle/1828/10212

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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