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1. Erdogan, Fettah. Structural and Functional Studies of TraG From the F Plasmid and the Major Pilin From Coxiella Burnetii.

Degree: MSc -MS, Chemistry, 2017, York University

The Escherichia coli F-plasmid houses all genes necessary for the assembly of its conjugative machinery to facilitate successful DNA transfer. TraG is one of the largest proteins (102 kDa) encoded in the transfer (tra) region of the F-plasmid that facilitates conjugative transfer. The N-terminal region of TraG is involved in pilus synthesis and assembly, while the C-terminal periplasmic domain is required for plasmid-specific entry exclusion and mating pair stabilization. In order to provide structural insights into TraGs function, we have expressed, purified and initiated crystallization studies of the C-terminal region of TraG (designated TraG*, 52.7 kDa) and have obtained initial crystals of TraG*. Our experiments on functional and dynamic characterization of TraG* using liquid chromatography coupled mass spectrometry (LC-MS), size exclusion chromatography coupled multi-angle light scattering (SEC-MALS) and protein gel electrophoresis has given significant insight into TraG* crystallization strategies as well as potential TraG mechanism of action during conjugation. Advisors/Committee Members: Audette, Gerald F. (advisor).

Subjects/Keywords: Chemistry; X ray crystallography; TraG; F plasmid; Tra region; Transfer protein; Escherichia coli; E coli; Coxiella burnettii; TraG*; Truncated ulp1; Structural biochemistry; Biological chemistry; cbu0156; pilA; T4SS; T2SS; Secretion systems; Transfer systems; Bacterial conjugation; F conjugation; Pilin; Pili; Mating pair stabilization; Mating pair formation; Entry exclusion; Pilin synthesis and assembly

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APA (6th Edition):

Erdogan, F. (2017). Structural and Functional Studies of TraG From the F Plasmid and the Major Pilin From Coxiella Burnetii. (Masters Thesis). York University. Retrieved from http://hdl.handle.net/10315/33351

Chicago Manual of Style (16th Edition):

Erdogan, Fettah. “Structural and Functional Studies of TraG From the F Plasmid and the Major Pilin From Coxiella Burnetii.” 2017. Masters Thesis, York University. Accessed September 18, 2019. http://hdl.handle.net/10315/33351.

MLA Handbook (7th Edition):

Erdogan, Fettah. “Structural and Functional Studies of TraG From the F Plasmid and the Major Pilin From Coxiella Burnetii.” 2017. Web. 18 Sep 2019.

Vancouver:

Erdogan F. Structural and Functional Studies of TraG From the F Plasmid and the Major Pilin From Coxiella Burnetii. [Internet] [Masters thesis]. York University; 2017. [cited 2019 Sep 18]. Available from: http://hdl.handle.net/10315/33351.

Council of Science Editors:

Erdogan F. Structural and Functional Studies of TraG From the F Plasmid and the Major Pilin From Coxiella Burnetii. [Masters Thesis]. York University; 2017. Available from: http://hdl.handle.net/10315/33351

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