subject:(Tip Enhanced Raman spectroscopie)

1. Touzalin, Thomas. Tip-enhanced Raman spectroscopy on electrochemical systems : Spectroscopie Raman exaltée de pointe pour les systèmes électrochimiques.

Degree: Docteur es, Chimie physique, 2018, Sorbonne université

URL: http://www.theses.fr/2018SORUS364

►

L'analyse in situ d'interfaces électrochimiques à l'échelle nanométriques est un enjeu majeur pour la compréhension des mécanismes de transferts de charges et d'électrons dans les… (more)

▼

L'analyse in situ d'interfaces électrochimiques à l'échelle nanométriques est un enjeu majeur pour la compréhension des mécanismes de transferts de charges et d'électrons dans les domaines du stockage d'énergie ou de l'électrocatalyse. Ce travail a permis le développement de la spectroscopie Raman exaltée de pointe (TERS) en milieu liquide et en conditions électrochimiques. Le TERS permet l'analyse de la structure de molécules ou de matériaux à l'échelle nanométrique du fait de l'exaltation localisée du champ électrique à l'extrémité d'une sonde de microscope à effet tunnel (STM) en or ou en argent. Un dispositif reposant sur l'illumination d'une pointe au travers d'un solvant organique a démontré la possibilité d'imager les inhomogénéités d'une monocouche auto-assemblée sur or. Une seconde approche reposant sur l'exaltation du signal Raman à l'apex d'une pointe de taille nanométrique utilisée comme microélectrode (spectroscopie Raman exaltée de surface de pointe, tip SERS) a permis de suivre la réduction d'une monocouche auto-assemblée et d'améliorer la compréhension de son mécanisme. Afin d'imager la surface d'une électrode polarisée, le couplage d'un STM utilisant une pointe TERS en conditions électrochimiques a montré une résolution latérale de moins de 8 nm pour sonder de variations locales de l'exaltation du champ électromagnétique induites par des singularités géométriques de surface. Par ailleurs, l'analyse TERS de couches organiques formées à partir de sels d'aryldiazoniums a permis de montrer des différences de structures selon type de greffage. Ce travail constitue donc une avancée majeure pour l'analyse locale de surfaces modifiées.

The in situ investigation of electrochemical interfaces structures at the nanoscale is a key element in the understanding of charge and electron transfer mechanisms e.g. in the fields of energy storage or electrocatalysis. This thesis introduces the implementation of tip-enhanced Raman spectroscopy (TERS) in liquid and in electrochemical conditions enabling the nanoscale analysis of electrified solid/liquid interfaces through the strong and local electric field enhancement at gold or silver scanning tunneling microscopy (STM) probes. The ability of TERS to image inhomogeneities in the coverage density of a self-assembled monolayer (SAM) through a layer of organic solvent on gold was demonstrated. A TERS-inspired analytical tool was also developed, based on a TERS tip used simultaneously as a single-hot spot surface-enhanced Raman spectroscopy (SERS) platform and as a microelectrode (EC tip SERS). The reduction of an electroactive SAM could then be monitored by electrochemical and in situ SERS measurements. In situ electrochemical STM-TERS was also evidenced through the imaging of local variations of the electric field enhancement on peculiar sites of a gold electrode with a lateral resolution lower than 8 nm. Finally TERS also demonstrated to be efficient in investigating the structure of organic layers grafted either by electrochemical reduction or spontaneously. This work…

Advisors/Committee Members: Maisonhaute, Emmanuel (thesis director), Lucas, Ivan (thesis director).

Subjects/Keywords: Spectroscopie Raman exaltée de pointe (TERS); Spectroscopie Raman exaltée de surface (SERS); Électrochimie; Sels de diazoniums; 4-Nitrothiophenol; Fonctionnalisation de surface; Tip-enhanced Raman spectroscopy (TERS); Surface-enhanced Raman spectroscopy (SERS); Electrochemistry; Diazonium salts; 4-Nitrothiophenol; Surface functionalization; 541.37

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Touzalin, T. (2018). Tip-enhanced Raman spectroscopy on electrochemical systems : Spectroscopie Raman exaltée de pointe pour les systèmes électrochimiques. (Doctoral Dissertation). Sorbonne université. Retrieved from http://www.theses.fr/2018SORUS364

Chicago Manual of Style (16^th Edition):

Touzalin, Thomas. “Tip-enhanced Raman spectroscopy on electrochemical systems : Spectroscopie Raman exaltée de pointe pour les systèmes électrochimiques.” 2018. Doctoral Dissertation, Sorbonne université. Accessed January 15, 2021. http://www.theses.fr/2018SORUS364.

MLA Handbook (7^th Edition):

Touzalin, Thomas. “Tip-enhanced Raman spectroscopy on electrochemical systems : Spectroscopie Raman exaltée de pointe pour les systèmes électrochimiques.” 2018. Web. 15 Jan 2021.

Vancouver:

Touzalin T. Tip-enhanced Raman spectroscopy on electrochemical systems : Spectroscopie Raman exaltée de pointe pour les systèmes électrochimiques. [Internet] [Doctoral dissertation]. Sorbonne université; 2018. [cited 2021 Jan 15]. Available from: http://www.theses.fr/2018SORUS364.

Council of Science Editors:

Touzalin T. Tip-enhanced Raman spectroscopy on electrochemical systems : Spectroscopie Raman exaltée de pointe pour les systèmes électrochimiques. [Doctoral Dissertation]. Sorbonne université; 2018. Available from: http://www.theses.fr/2018SORUS364

Dalhousie University

2. Gullekson, Corinne. Surface enhanced Raman spectroscopy of collagen I fibrils.

Degree: MS, Department of Physics & Atmospheric Science, 2011, Dalhousie University

URL: http://hdl.handle.net/10222/14026

► Collagen fibrils are the main constituent of the extracellular matrix surrounding eukaryotic cells. Even though the assembly and structure of collagen fibrils is well characterized,… (more)

Subjects/Keywords: Raman; surface-enhanced Raman; tip-enhanced Raman; fibrous protein; amino acid; gold; silver

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Gullekson, C. (2011). Surface enhanced Raman spectroscopy of collagen I fibrils. (Masters Thesis). Dalhousie University. Retrieved from http://hdl.handle.net/10222/14026

Chicago Manual of Style (16^th Edition):

Gullekson, Corinne. “Surface enhanced Raman spectroscopy of collagen I fibrils.” 2011. Masters Thesis, Dalhousie University. Accessed January 15, 2021. http://hdl.handle.net/10222/14026.

MLA Handbook (7^th Edition):

Gullekson, Corinne. “Surface enhanced Raman spectroscopy of collagen I fibrils.” 2011. Web. 15 Jan 2021.

Vancouver:

Gullekson C. Surface enhanced Raman spectroscopy of collagen I fibrils. [Internet] [Masters thesis]. Dalhousie University; 2011. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/10222/14026.

Council of Science Editors:

Gullekson C. Surface enhanced Raman spectroscopy of collagen I fibrils. [Masters Thesis]. Dalhousie University; 2011. Available from: http://hdl.handle.net/10222/14026

University of California – Irvine

3. Crampton, Kevin Thomas. Single molecules at plasmonic nanojunctions interrogated through space, time and frequency domain Raman scattering.

Degree: Chemistry, 2018, University of California – Irvine

URL: http://www.escholarship.org/uc/item/0rf4s5qp

► Surface-enhanced vibrational spectroscopy has emerged as a powerful tool to probe the (photo)chemistry and (photo)physics of individual molecules, which may be regarded as the primary… (more)

▼ Surface-enhanced vibrational spectroscopy has emerged as a powerful tool to probe the (photo)chemistry and (photo)physics of individual molecules, which may be regarded as the primary focus of the field of nanophotonics. Single molecule (SM) sensitivity relies on the excitation of localized surface plasmons which confine far-field radiation to molecular length scales, ultimately enabling submolecular imaging with chemical selectivity. Over the length scales that enable submolecular resolution, localized optical fields cannot be separated from the charge density oscillations that sustain them. As such, the interplay between plasmons, photons and electrons in plasmonic nano-junctions is of critical importance for the development of imaging modalities capable of circumventing the diffraction limit and, more generally, for gaining broader insight into the fundamental mechanisms of surface-enhanced Raman and tip-enhanced Raman scattering (SERS and TERS). In what follows, the SM surface-enhanced parameter space is explored through linear and ultrafast optical measurements utilizing nanometer to ångstrom scale plasmonic junctions and non-resonant molecular reporters. SERS measurements carried out on the prototypical gold dimer nano-antenna highlight the interplay between the molecular Raman scattering channel(s) and coherent luminescence of the plasmon itself. Ultrafast studies utilizing 100 femtosecond laser pulses establish the operating principles of the ultrafast analog of surface-enhanced spectroscopy, which are then utilized to demonstrated time and frequency resolved coherent Raman scattering (CRS) in the single molecule limit.The fundamental mechanism of SERS is explored by replacing the continuous-wave excitation source with a picosecond pulse train, which probes the non-equilibrium population dynamics of SM phonon states. The latter are inferred through the observed inverted Stokes/anti-Stokes ratios and verified through stimulated and coherent Raman scattering (SRS and CARS) measurements carried out on the same system. Direct plasmon-molecule energy transfer is dominant over the optically induced and thermal contributions to the vibrational population. Finally, SM TERS measurements utilizing a metalloporphyrin functionalized silver tip are carried out in ultrahigh vacuum and ultralow temperatures (5 K). These measurements directly image the atomic lattice of a copper nitride monolayer with 1.5 Å precision. A series of measurements recorded on this system highlight the essential role of photoelectrons in the TERS process.

Subjects/Keywords: Physical chemistry; coherent Raman; plasmon; Raman; single molecule; surface enhanced Raman scattering; tip enhanced Raman scattering

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Crampton, K. T. (2018). Single molecules at plasmonic nanojunctions interrogated through space, time and frequency domain Raman scattering. (Thesis). University of California – Irvine. Retrieved from http://www.escholarship.org/uc/item/0rf4s5qp

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Crampton, Kevin Thomas. “Single molecules at plasmonic nanojunctions interrogated through space, time and frequency domain Raman scattering.” 2018. Thesis, University of California – Irvine. Accessed January 15, 2021. http://www.escholarship.org/uc/item/0rf4s5qp.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Crampton, Kevin Thomas. “Single molecules at plasmonic nanojunctions interrogated through space, time and frequency domain Raman scattering.” 2018. Web. 15 Jan 2021.

Vancouver:

Crampton KT. Single molecules at plasmonic nanojunctions interrogated through space, time and frequency domain Raman scattering. [Internet] [Thesis]. University of California – Irvine; 2018. [cited 2021 Jan 15]. Available from: http://www.escholarship.org/uc/item/0rf4s5qp.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Crampton KT. Single molecules at plasmonic nanojunctions interrogated through space, time and frequency domain Raman scattering. [Thesis]. University of California – Irvine; 2018. Available from: http://www.escholarship.org/uc/item/0rf4s5qp

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Texas A&M University

4. Chang, Chi-Yuan 1980-. Electrical and Optical Characterization of Nanoscale Materials for Electronics.

Degree: PhD, Chemistry, 2012, Texas A&M University

URL: http://hdl.handle.net/1969.1/148119

► Due to a lack of fundamental knowledge about the role of molecular structures in molecular electronic devices, this research is focused on the development of… (more)

▼ Due to a lack of fundamental knowledge about the role of molecular structures in molecular electronic devices, this research is focused on the development of instruments to understand the relation between device design and the electronic properties of electroactive components. The overall goal is to apply this insight to obtain a more efficient and reliable scheme and greater functional control over each component. This work developed a fabrication method for porphyrinoids on graphene-based field effect transistors (FETs), and a chemical sensing platform under an ambient environment by integrating a tip-enhanced Raman spectroscope (TERS), atomic force microscope (AFM), and electronic testing circuit. The study is divided into three aspects. The first is aimed at demonstrating fabrication processes of nanoscale FETs of graphene and porphyrinoid composites based entirely on scanning probe lithography (SPL). A nanoshaving mechanism was used to define patterns on octadecanethiol self-assembled monolayers on gold film evaporated on graphene flakes, followed by metal wet etching and/or oxygen plasma etching to develop patterns on Au films and graphene, respectively. The integrity and optoelectronic properties were examined to validate the processes. The second area of study focused on the development of the chemical sensing platform, enabling chemical changes to be monitored during charge transports under an ambient environment. The localized Raman enhancement was induced by exciting surface plasmon resonance in nanoscale silver enhancing probes made by thermal silver evaporation on sharp AFM tips. As the system was designed along an off-axis illumination/collection scheme, it was demonstrated that it was capable of observing molecular decomposition on opaque and conductive substrates induced by an electric bias. The third line of work proposed a novel TERS system and a probe preparation method. Silver nanowires mounted on AFM tips were used to locally enhance the Raman scattering. The observed Raman enhancement allows quick chemical analysis from a nanoscale region, and thus enables chemical mapping beyond the diffraction limit. Compared with other TERS geometries, the new optical design not only allows analysis on large or opaque samples, but also simplifies the design of the optical components and the alignment processes of the setup. Advisors/Committee Members: Batteas, James D (advisor), Son, Dong Hee (committee member), Liang, Hong (committee member), Cremer, Paul S (committee member).

Subjects/Keywords: Nanowire; Nanolithography; FET; TERS; Tip-enhanced Raman; Graphene

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Chang, C. 1. (2012). Electrical and Optical Characterization of Nanoscale Materials for Electronics. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/148119

Chicago Manual of Style (16^th Edition):

Chang, Chi-Yuan 1980-. “Electrical and Optical Characterization of Nanoscale Materials for Electronics.” 2012. Doctoral Dissertation, Texas A&M University. Accessed January 15, 2021. http://hdl.handle.net/1969.1/148119.

MLA Handbook (7^th Edition):

Chang, Chi-Yuan 1980-. “Electrical and Optical Characterization of Nanoscale Materials for Electronics.” 2012. Web. 15 Jan 2021.

Vancouver:

Chang C1. Electrical and Optical Characterization of Nanoscale Materials for Electronics. [Internet] [Doctoral dissertation]. Texas A&M University; 2012. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/1969.1/148119.

Council of Science Editors:

Chang C1. Electrical and Optical Characterization of Nanoscale Materials for Electronics. [Doctoral Dissertation]. Texas A&M University; 2012. Available from: http://hdl.handle.net/1969.1/148119

Baylor University

5. Birmingham, Blake, 1991-. Probing interactions among molecules, substrate, and tip using tip-enhanced Raman spectroscopy.

Degree: MS, Baylor University. Dept. of Physics., 2017, Baylor University

URL: http://hdl.handle.net/2104/10160

► Tip-Enhanced Raman Spectroscopy (TERS) has shown that spatial resolution <1 nm is possible, but is technically challenging and difficult even in Ultra-High Vacuum at low… (more)

Subjects/Keywords: Raman. Tip-enhanced. MoS2. CuPc. Phthalocyanine. Au. AFM. STM.

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Birmingham, Blake, 1. (2017). Probing interactions among molecules, substrate, and tip using tip-enhanced Raman spectroscopy. (Masters Thesis). Baylor University. Retrieved from http://hdl.handle.net/2104/10160

Chicago Manual of Style (16^th Edition):

Birmingham, Blake, 1991-. “Probing interactions among molecules, substrate, and tip using tip-enhanced Raman spectroscopy.” 2017. Masters Thesis, Baylor University. Accessed January 15, 2021. http://hdl.handle.net/2104/10160.

MLA Handbook (7^th Edition):

Birmingham, Blake, 1991-. “Probing interactions among molecules, substrate, and tip using tip-enhanced Raman spectroscopy.” 2017. Web. 15 Jan 2021.

Vancouver:

Birmingham, Blake 1. Probing interactions among molecules, substrate, and tip using tip-enhanced Raman spectroscopy. [Internet] [Masters thesis]. Baylor University; 2017. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/2104/10160.

Council of Science Editors:

Birmingham, Blake 1. Probing interactions among molecules, substrate, and tip using tip-enhanced Raman spectroscopy. [Masters Thesis]. Baylor University; 2017. Available from: http://hdl.handle.net/2104/10160

University of California – Irvine

6. Tallarida, Nicholas Ryan. Single Molecule Studies with Scanning Tunneling Microscopy and Tip-Enhanced Raman Spectroscopy.

Degree: Chemistry, 2017, University of California – Irvine

URL: http://www.escholarship.org/uc/item/8gc5q8dq

► Experimental techniques with the sensitivity to measure properties of individual molecules present an opportunity to investigate and understand fundamental chemical and physical processes. One such… (more)

Subjects/Keywords: Physical chemistry; Plasmonics; Raman Spectroscopy; Scanning Tunneling Microscopy; Single Molecule; Tip-enhanced Raman spectroscopy

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Tallarida, N. R. (2017). Single Molecule Studies with Scanning Tunneling Microscopy and Tip-Enhanced Raman Spectroscopy. (Thesis). University of California – Irvine. Retrieved from http://www.escholarship.org/uc/item/8gc5q8dq

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Tallarida, Nicholas Ryan. “Single Molecule Studies with Scanning Tunneling Microscopy and Tip-Enhanced Raman Spectroscopy.” 2017. Thesis, University of California – Irvine. Accessed January 15, 2021. http://www.escholarship.org/uc/item/8gc5q8dq.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Tallarida, Nicholas Ryan. “Single Molecule Studies with Scanning Tunneling Microscopy and Tip-Enhanced Raman Spectroscopy.” 2017. Web. 15 Jan 2021.

Vancouver:

Tallarida NR. Single Molecule Studies with Scanning Tunneling Microscopy and Tip-Enhanced Raman Spectroscopy. [Internet] [Thesis]. University of California – Irvine; 2017. [cited 2021 Jan 15]. Available from: http://www.escholarship.org/uc/item/8gc5q8dq.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Tallarida NR. Single Molecule Studies with Scanning Tunneling Microscopy and Tip-Enhanced Raman Spectroscopy. [Thesis]. University of California – Irvine; 2017. Available from: http://www.escholarship.org/uc/item/8gc5q8dq

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Brno University of Technology

7. Šilhan, Lukáš. Příprava ultra ostrých hrotů pro STM - TERS aplikace: Preparation of ultra sharp tips for STM TERS applications.

Degree: 2019, Brno University of Technology

URL: http://hdl.handle.net/11012/61043

► The goal of this bachelor thesis is preparation of Au tips by electrochemical etching. The theoretical part deals with comparison of microscopy techniques for materials… (more)

Subjects/Keywords: Mikroskopie; Au hroty; Elektrochemické leptání; Ramanova spektroskopie; RS; Tip-enhanced Raman spectroscopy; TERS; Microscopy; Au tips; Electrochemical ething; Raman spectroscopy; RS; Tip-enhanced Raman spectroscopy; TERS

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Šilhan, L. (2019). Příprava ultra ostrých hrotů pro STM - TERS aplikace: Preparation of ultra sharp tips for STM TERS applications. (Thesis). Brno University of Technology. Retrieved from http://hdl.handle.net/11012/61043

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Šilhan, Lukáš. “Příprava ultra ostrých hrotů pro STM - TERS aplikace: Preparation of ultra sharp tips for STM TERS applications.” 2019. Thesis, Brno University of Technology. Accessed January 15, 2021. http://hdl.handle.net/11012/61043.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Šilhan, Lukáš. “Příprava ultra ostrých hrotů pro STM - TERS aplikace: Preparation of ultra sharp tips for STM TERS applications.” 2019. Web. 15 Jan 2021.

Vancouver:

Šilhan L. Příprava ultra ostrých hrotů pro STM - TERS aplikace: Preparation of ultra sharp tips for STM TERS applications. [Internet] [Thesis]. Brno University of Technology; 2019. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/11012/61043.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Šilhan L. Příprava ultra ostrých hrotů pro STM - TERS aplikace: Preparation of ultra sharp tips for STM TERS applications. [Thesis]. Brno University of Technology; 2019. Available from: http://hdl.handle.net/11012/61043

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

8. Aybeke, Ece Neslihan. Study of the dynamics of biomolecules by high speed atomic force microscopy and surface enhanced Raman spectroscopy : L'étude dynamique des biomolécules par le microscope à force atomique haute-vitesse (HS-AFM) et la spectroscopie Raman exaltée de surface (SERS).

Degree: Docteur es, Physique, 2015, Université de Bourgogne

URL: http://www.theses.fr/2015DIJOS023

►

Ce travail de thèse se focalise sur le couplage du microscope à force atomique haute–vitesse (HS-AFM) et de la spectroscopie Raman exaltée de surface (SERS)… (more)

▼

Ce travail de thèse se focalise sur le couplage du microscope à force atomique haute–vitesse (HS-AFM) et de la spectroscopie Raman exaltée de surface (SERS) pour la détection des biomolécules. Nous avons élaboré un protocole de fabrication pour produire les substrats “SERS-actifs”. L’efficacité des substrats de nanoparticules cristalline d’or, d’argent ou bimétallique argent–or a été évaluée. Nous avons étudié l’impact des propriétés optiques et morphologiques des substrats sur l’intensité Raman en analysant des échantillons tests tels que la bipyridine éthylène et le bleu de méthylène. Nous nous sommes interessés à trois problematiques biologiques distinctes par analyses HS-AFM et SERS. Dans un premier cas, nous avons détecté la signature chimique de protéine cytochrome b5. Ce travail a été suivi par des études sur le changement de conformation de la protéine de choc thermique leuconostoc oenos Lo 18 en fonction de la concentration et du pH. La dernière application consiste en l’analyse des interactions membrane – virus. Afin de réaliser les analyses simultanées Raman/AFM, nous avons adapté notre protocole de fabrication pour couvrir la surface des pointes AFM commerciales par des nanoparticules d’or cristallines. Les études de diffusion Raman exaltée par effet de pointe (TERS) ont été effectuées sur les échantillons de disulfure de molybdène pour évaluer la qualité des pointes TERS. Pour finir, nous présentons une nouvelle configuration de couplage HS-AFM et spectroscopie Raman. Nous discutons des modifications et des défis rencontrés.

This thesis focuses on the coupling of High–Speed Atomic Force Microscopy (HS-AFM) and Surface Enhanced Raman Spectroscopy (SERS) for biomolecule analysis. We have designed a fabrication protocol to manufacture “SERS-active” substrates. The efficacy of gold, silver and gold-silver bimetallic crystalline nanoparticle substrates were evaluated. We have investigated the impact of optical and morphological features of the substrates on Raman signal intensity by analyzing well-known samples such as bipyridine ethylene and methylene blue molecules. We took an interest in three distinct biological problematics with HS-AFM and SERS analyses. First, we have detected the chemical signature of cytochrome b5 protein. This study was followed by the investigation of conformational changes of small heat shock leuconostoc oenos Lo 18 protein in function of pH level and concentrations. The last application consists to the analyse a membrane and a virus interaction. In order to realize simultaneous Raman/AFM analysis, we have adapted our fabrication protocol to cover the surface of commercial AFM probes by crystalline gold nanoparticles. Tip – Enhanced Raman Spectroscopy (TERS) studies were performed on molybdenum disulfide to evaluate the quality of TERS probes. In the last part of this work, we have designed a new setup to combine Ando’s HS-AFM setup with Raman spectroscopy. We present the modifications that have been carried out and the challenges that we have encountered.

Advisors/Committee Members: Lesniewska, Eric (thesis director), Bourillot, Eric (thesis director).

Subjects/Keywords: Microscopie à force atomique haute-vitesse (HS-AFM); Spectroscopie Raman exaltée de surface (SERS); Diffusion Raman exaltée par effet de pointe (TERS); Substrats de nanoparticules; Plasmons de Surface Localisé (LSP); Cellules; Protéines; Détergent résistant membrane domaines (DRMs); Noroviruses (NoVs); High–Speed Atomic Force Microscopy (HS-AFM); Surface Enhanced Raman Spectroscopy (SERS); Tip–Enhanced Raman Spectroscopy (TERS); Nanoparticle substrates; Localized Surface Plasmons (LSP); Cells; Proteins; Detergent resistant membrane domains (DRMs); Noroviruses (NoVs); 539; 620.5

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Aybeke, E. N. (2015). Study of the dynamics of biomolecules by high speed atomic force microscopy and surface enhanced Raman spectroscopy : L'étude dynamique des biomolécules par le microscope à force atomique haute-vitesse (HS-AFM) et la spectroscopie Raman exaltée de surface (SERS). (Doctoral Dissertation). Université de Bourgogne. Retrieved from http://www.theses.fr/2015DIJOS023

Chicago Manual of Style (16^th Edition):

Aybeke, Ece Neslihan. “Study of the dynamics of biomolecules by high speed atomic force microscopy and surface enhanced Raman spectroscopy : L'étude dynamique des biomolécules par le microscope à force atomique haute-vitesse (HS-AFM) et la spectroscopie Raman exaltée de surface (SERS).” 2015. Doctoral Dissertation, Université de Bourgogne. Accessed January 15, 2021. http://www.theses.fr/2015DIJOS023.

MLA Handbook (7^th Edition):

Aybeke, Ece Neslihan. “Study of the dynamics of biomolecules by high speed atomic force microscopy and surface enhanced Raman spectroscopy : L'étude dynamique des biomolécules par le microscope à force atomique haute-vitesse (HS-AFM) et la spectroscopie Raman exaltée de surface (SERS).” 2015. Web. 15 Jan 2021.

Vancouver:

Aybeke EN. Study of the dynamics of biomolecules by high speed atomic force microscopy and surface enhanced Raman spectroscopy : L'étude dynamique des biomolécules par le microscope à force atomique haute-vitesse (HS-AFM) et la spectroscopie Raman exaltée de surface (SERS). [Internet] [Doctoral dissertation]. Université de Bourgogne; 2015. [cited 2021 Jan 15]. Available from: http://www.theses.fr/2015DIJOS023.

Council of Science Editors:

Aybeke EN. Study of the dynamics of biomolecules by high speed atomic force microscopy and surface enhanced Raman spectroscopy : L'étude dynamique des biomolécules par le microscope à force atomique haute-vitesse (HS-AFM) et la spectroscopie Raman exaltée de surface (SERS). [Doctoral Dissertation]. Université de Bourgogne; 2015. Available from: http://www.theses.fr/2015DIJOS023

University of California – Irvine

9. Tallarida, Nicholas Ryan. Characterization and Analysis of Conductance: From Polymers to Single Electrons.

Degree: Chemical and Material Physics, 2016, University of California – Irvine

URL: http://www.escholarship.org/uc/item/5b70q4gf

► Experimental studies on the conductive properties of molecular systems led to the discovery and characterization of two essential elements of molecular electronics: a molecularwire and… (more)

Subjects/Keywords: Physical chemistry; conductive polymer; electronic switching; scanning tunneling microscopy; single molecule; Tip enhanced raman spectroscopy

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Tallarida, N. R. (2016). Characterization and Analysis of Conductance: From Polymers to Single Electrons. (Thesis). University of California – Irvine. Retrieved from http://www.escholarship.org/uc/item/5b70q4gf

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Tallarida, Nicholas Ryan. “Characterization and Analysis of Conductance: From Polymers to Single Electrons.” 2016. Thesis, University of California – Irvine. Accessed January 15, 2021. http://www.escholarship.org/uc/item/5b70q4gf.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Tallarida, Nicholas Ryan. “Characterization and Analysis of Conductance: From Polymers to Single Electrons.” 2016. Web. 15 Jan 2021.

Vancouver:

Tallarida NR. Characterization and Analysis of Conductance: From Polymers to Single Electrons. [Internet] [Thesis]. University of California – Irvine; 2016. [cited 2021 Jan 15]. Available from: http://www.escholarship.org/uc/item/5b70q4gf.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Tallarida NR. Characterization and Analysis of Conductance: From Polymers to Single Electrons. [Thesis]. University of California – Irvine; 2016. Available from: http://www.escholarship.org/uc/item/5b70q4gf

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Freie Universität Berlin

10. Schambach, Philip. Spitzenverstärkte Raman-Spektroskopie im Ultrahochvakuum.

Degree: 2013, Freie Universität Berlin

URL: http://dx.doi.org/10.17169/refubium-13826

► Spitzenverstärkte Raman-Spektroskopie (tip-enhanced Raman spectroscopy, TERS) ist eine vielversprechende, neue Rastersondentechnik für die Untersuchung von Schwingungseigenschaften adsorbierter Moleküle. TERS verfügt über eine hohe Empfindlichkeit, bis… (more)

▼ Spitzenverstärkte Raman-Spektroskopie (tip-enhanced Raman spectroscopy, TERS) ist eine vielversprechende, neue Rastersondentechnik für die Untersuchung von Schwingungseigenschaften adsorbierter Moleküle. TERS verfügt über eine hohe Empfindlichkeit, bis hin zur Einzelmoleküldetektion, bei einer Auflösung im 10 nm-Bereich. Im Gegensatz zu den meisten TERS-Studien führen wir unsere Experimente im Ultrahochvakuum (UHV) durch. Um eine gute Probenqualität zu gewährleisten, wurde die bestehende UHV-TERS Apparatur um eine Präparationskammer erweitert. Ein substantieller Teil der Arbeit bestand in der Erweiterung der experimentellen Möglichkeiten, sowie der Handhabbarkeit des UHV-TERS Gerätes. Wir haben das Fulleren C60 auf einer Au(111) Oberfläche als Probensystem gewählt. Aus der umfangreichen Literatur sind die Schwingungs- und Adsorptionseigenschaften von C60 bekannt. Im Gegensatz zu in anderen UHV-TERS Experimenten verwendeten Molekülen, hat C60 einen sehr kleinen Raman Streuquerschnitt. Dieser Sachverhalt erlaubt es, die Möglichkeiten unseres Experiments für eine große Klasse von Adsorbaten mit vergleichbar niedrigen Streuquerschnitten zu untersuchen. Um Signale von C60 und Fremdmolekülen voneinander zu trennen, haben wir zeitaufgelöste Reihen von spitzenverstärkten Raman (TER) Spektren aufgenommen. Nur die für C60 charakteristische Spektren, die über mehrere Sekunden bis Minuten stabil waren, wurden zur Auswertung herangezogen. Die Abhängigkeit der TER Spektren von C60 auf Au(111) von der zwischen Spitze und Probe angelegten Spannung weist auf einen erheblichen Einfluss des Spitze-Probe-Abstandes auf die Signalstärke hin, auch im Sub-Nanometersbereich. Unterschiedliche C60-Bedeckungen ergeben Energieverschiebungen einiger TERS-Banden. Dies wird im Zusammenhang mit verschiedenen Adsorptionsplätzen und einer möglichen Polymerisierung der C60-Schichten diskutiert. Zuletzt werden Experimente präsentiert, in denen rastertunnelmikroskopische Bilder von C60 auf Au(111) und TER Spektren an der gleichen Stelle der Probe aufgenommen wurden. Diese Ergebnisse liefern einen Ausblick auf Korrelation von struktureller und chemischer Information im nm Bereich. Advisors/Committee Members: [email protected] (contact), m (gender), Dr. Bruno Pettinger und Prof. Dr. Martin Wolf (firstReferee), Prof. Dr. Stephanie Reich (furtherReferee).

Subjects/Keywords: Tip-enhanced Raman spectroscopy; C60; TERS; UHV-TERS; 500 Naturwissenschaften und Mathematik::530 Physik

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Schambach, P. (2013). Spitzenverstärkte Raman-Spektroskopie im Ultrahochvakuum. (Thesis). Freie Universität Berlin. Retrieved from http://dx.doi.org/10.17169/refubium-13826

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Schambach, Philip. “Spitzenverstärkte Raman-Spektroskopie im Ultrahochvakuum.” 2013. Thesis, Freie Universität Berlin. Accessed January 15, 2021. http://dx.doi.org/10.17169/refubium-13826.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Schambach, Philip. “Spitzenverstärkte Raman-Spektroskopie im Ultrahochvakuum.” 2013. Web. 15 Jan 2021.

Vancouver:

Schambach P. Spitzenverstärkte Raman-Spektroskopie im Ultrahochvakuum. [Internet] [Thesis]. Freie Universität Berlin; 2013. [cited 2021 Jan 15]. Available from: http://dx.doi.org/10.17169/refubium-13826.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Schambach P. Spitzenverstärkte Raman-Spektroskopie im Ultrahochvakuum. [Thesis]. Freie Universität Berlin; 2013. Available from: http://dx.doi.org/10.17169/refubium-13826

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Penn State University

11. Liu, Pengchong. Interpreting High-Resolution Tip-Enhanced Raman Spectroscopy Images.

Degree: 2020, Penn State University

URL: https://submit-etda.libraries.psu.edu/catalog/17830pbl5075

► Raman spectroscopy is one of the fundamental techniques in chemical characterization. It measures the amount of photons and their frequency shifts scattered by the molecule… (more)

▼ Raman spectroscopy is one of the fundamental techniques in chemical characterization. It measures the amount of photons and their frequency shifts scattered by the molecule under a given laser beam. The frequency shifts originate from the molecule’s vibrations, which are determined by its microscopic structure. Thus, a Raman spectrum is often regarded as the molecule’s "fingerprint". When the molecule is placed on a noble metal surface with some curvature, the Raman signals are greatly enhanced by the plasmonic near field at the surface. This technique is called "surface- enhanced Raman spectroscopy", or SERS. Because of the significantly improved sensitivity relative to regular Raman scattering, SERS has grown into a widely popular tool for chemical research and practical applications. Moreover, the molecule-metal interaction that lies in the center of the mechanism of SERS has been a hot topic of fundamental research since its discovery and has led to many variants in the development of spectroscopic techniques. Tip-enhanced Raman spectroscopy (TERS) integrates the scanning probe microscopy and SERS. Similar to SERS, the enhancement of Raman scattering in TERS benefits from the inhomogeneous near field induced by the plasmonic resonance of the noble metal nanostructure. However, the TERS spectrum of a molecule is often found different than SERS, and it changes with the tip position. The combination of the chemical sensitivity and the spatial resolution makes TERS a promising technique for microscopic characterization. A comprehensive demonstration of these unique features is TERS imaging. A TERS image is a two-dimensional map of the Raman intensity at a certain frequency varying with the scanning tip. It exhibits a sub-nanometer resolution and is specific to the corresponding vibrational mode. TERS imaging is considered as a visualization of molecular vibrations and sets stage for a new way of understanding molecules. Therefore, it is necessary to develop theoretical interpretation of TERS images and their connections to the underlying molecular vibration. However, interpreting TERS images is challenging as the brightspot pattern does not always strictly align with the vibrating atoms and the signals are very sensitive to experimental conditions. In this dissertation, three major aspects of TERS theory are explored: the effect of the plasmonic near field electromagnetic mechanism), the plasmonic resonance inside a subnanometer cavity (gap plasmonics), and the quantum mechanical interactions between the molecule and the substrate (the chemical mechanism). In particular, the critical conditions of the near field to achieve atomic resolution are found. It is also illustrated that the locally integrated Raman polarizability density is the sub-molecular property probed by the confined near field in TERS. It provides an intuitive description of the TERS imaging mechanism, which is usually hidden in the self-consistent cycles of conventional calculations using the time-dependent density functional theory. The distinct… Advisors/Committee Members: Lasse Jensen, Dissertation Advisor/Co-Advisor, Lasse Jensen, Committee Chair/Co-Chair, William George Noid, Committee Member, Paul S Cremer, Committee Member, Adri C.T. van Duin, Outside Member, Philip C Bevilacqua, Program Head/Chair.

Subjects/Keywords: Tip-Enhanced Raman Spectroscopy; Gap Plasmonics; Raman Polarizability Density; Frozen Density Embedding; Atomistic Electrodynamics; Near Field

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Liu, P. (2020). Interpreting High-Resolution Tip-Enhanced Raman Spectroscopy Images. (Thesis). Penn State University. Retrieved from https://submit-etda.libraries.psu.edu/catalog/17830pbl5075

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Liu, Pengchong. “Interpreting High-Resolution Tip-Enhanced Raman Spectroscopy Images.” 2020. Thesis, Penn State University. Accessed January 15, 2021. https://submit-etda.libraries.psu.edu/catalog/17830pbl5075.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Liu, Pengchong. “Interpreting High-Resolution Tip-Enhanced Raman Spectroscopy Images.” 2020. Web. 15 Jan 2021.

Vancouver:

Liu P. Interpreting High-Resolution Tip-Enhanced Raman Spectroscopy Images. [Internet] [Thesis]. Penn State University; 2020. [cited 2021 Jan 15]. Available from: https://submit-etda.libraries.psu.edu/catalog/17830pbl5075.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Liu P. Interpreting High-Resolution Tip-Enhanced Raman Spectroscopy Images. [Thesis]. Penn State University; 2020. Available from: https://submit-etda.libraries.psu.edu/catalog/17830pbl5075

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Manchester

12. Davies, Heather. Mucins in the alimentary canal : their structure and interactions with polyphenols.

Degree: PhD, 2014, University of Manchester

URL: https://www.research.manchester.ac.uk/portal/en/theses/mucins-in-the-alimentary-canal-their-structure-and-interactions-with-polyphenols(76aaa531-bf78-4be1-94a7-c8b4db9114bb).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.677729

► The polymeric gel-forming mucins provide the structural framework of saliva and the mucus barriers that cover the mucosal surfaces of the alimentary canal. Dietary compounds… (more)

▼ The polymeric gel-forming mucins provide the structural framework of saliva and the mucus barriers that cover the mucosal surfaces of the alimentary canal. Dietary compounds may influence the barrier properties of these protective layers. The effects of green tea polyphenols, which have many health benefits but have low bioavailability and contribute to the astringency of green tea, on the structural properties of the mucins in the alimentary canal are investigated here. Using well characterised, highly purified salivary mucins MUC5B and MUC7, and porcine gastric mucins, the effects of the green tea polyphenol epigallocatechin-3-gallate (EGCG) on mucins were studied here. Using rate-zonal centrifugation coupled to agarose gel electrophoresis, atomic force microscopy and particle tracking microrheology, EGCG, at concentrations found in a cup of green tea, caused increased aggregation of MUC5B in human whole saliva, and increased aggregation and viscosity of purified MUC5B. It was revealed using recombinant proteins of the N- and C-terminal regions of MUC5B that EGCG had these effects by aggregating the terminal globular protein domains of MUC5B. In contrast, MUC5B trypsin-resistant high molecular weight glycopeptides were not aggregated by EGCG, demonstrating that the oligosaccharide-rich, highly-glycosylated regions of mucins are not involved in the EGCG-induced aggregation of mucins. EGCG also caused the majority of MUC7 in human whole saliva to aggregate, and purified MUC7 also showed substantial aggregation in the presence of EGCG.Porcine gastric mucins were also used in order to model human gastric mucins. First, the identity of the porcine gastric mucins was explored using tandem mass spectrometry and immunohistochemistry. This revealed that Muc5ac was expressed by the surface epithelium and was the prominent mucin in porcine gastric mucus. Muc6 was expressed by gastric submucosal glands, but was not a major component of the secreted mucus barrier. Porcine Muc5ac and Muc6 were shown to be aggregated by EGCG. These data demonstrate that mucins from both saliva and the stomach are substantially altered by EGCG. This may contribute to the astringency and low bioavailability of EGCG. In contrast, the green tea polyphenol epicatechin (EC) did not cause aggregation of salivary mucins or porcine gastric mucins, suggesting that the galloyl ring of EGCG (which is absent in EC) is important for its aggregation of mucins, and that EC has different mechanisms of astringency. The structure of the mucins in the alimentary canal was studied using Raman spectroscopy, Raman optical activity (ROA) and Tip-enhanced Raman spectroscopy (TERS). The secondary structure of the oligosaccharide-rich regions of mucins was shown to be largely disordered, with some contribution of poly-proline II helix. The N- and C-terminal regions of MUC5B were largely β-sheet in structure, with some disordered structure also present in the C-terminal region. Raman spectroscopy could reliably distinguish between MUC5B glycoforms, demonstrating the…

Subjects/Keywords: 572; Mucin; Stomach; Mucus; Saliva; MUC5B; MUC7; Muc5ac; Muc6; Green tea polyphenols; Epigallocatechin-3-gallate; EGCG; Raman spectroscopy; Raman optical activity; Tip-enhanced Raman spectroscopy

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Davies, H. (2014). Mucins in the alimentary canal : their structure and interactions with polyphenols. (Doctoral Dissertation). University of Manchester. Retrieved from https://www.research.manchester.ac.uk/portal/en/theses/mucins-in-the-alimentary-canal-their-structure-and-interactions-with-polyphenols(76aaa531-bf78-4be1-94a7-c8b4db9114bb).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.677729

Chicago Manual of Style (16^th Edition):

Davies, Heather. “Mucins in the alimentary canal : their structure and interactions with polyphenols.” 2014. Doctoral Dissertation, University of Manchester. Accessed January 15, 2021. https://www.research.manchester.ac.uk/portal/en/theses/mucins-in-the-alimentary-canal-their-structure-and-interactions-with-polyphenols(76aaa531-bf78-4be1-94a7-c8b4db9114bb).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.677729.

MLA Handbook (7^th Edition):

Davies, Heather. “Mucins in the alimentary canal : their structure and interactions with polyphenols.” 2014. Web. 15 Jan 2021.

Vancouver:

Davies H. Mucins in the alimentary canal : their structure and interactions with polyphenols. [Internet] [Doctoral dissertation]. University of Manchester; 2014. [cited 2021 Jan 15]. Available from: https://www.research.manchester.ac.uk/portal/en/theses/mucins-in-the-alimentary-canal-their-structure-and-interactions-with-polyphenols(76aaa531-bf78-4be1-94a7-c8b4db9114bb).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.677729.

Council of Science Editors:

Davies H. Mucins in the alimentary canal : their structure and interactions with polyphenols. [Doctoral Dissertation]. University of Manchester; 2014. Available from: https://www.research.manchester.ac.uk/portal/en/theses/mucins-in-the-alimentary-canal-their-structure-and-interactions-with-polyphenols(76aaa531-bf78-4be1-94a7-c8b4db9114bb).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.677729

13. Davies, Heather. Mucins in the alimentary canal: their structure and interactions with polyphenols.

Degree: 2014, University of Manchester

URL: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:239764

► The polymeric gel-forming mucins provide the structural framework of saliva and the mucus barriers that cover the mucosal surfaces of the alimentary canal. Dietary compounds… (more)

▼ The polymeric gel-forming mucins provide the structural framework of saliva and the mucus barriers that cover the mucosal surfaces of the alimentary canal. Dietary compounds may influence the barrier properties of these protective layers. The effects of green tea polyphenols, which have many health benefits but have low bioavailability and contribute to the astringency of green tea, on the structural properties of the mucins in the alimentary canal are investigated here. Using well characterised, highly purified salivary mucins MUC5B and MUC7, and porcine gastric mucins, the effects of the green tea polyphenol epigallocatechin-3-gallate (EGCG) on mucins were studied here.Using rate-zonal centrifugation coupled to agarose gel electrophoresis, atomic force microscopy and particle tracking microrheology, EGCG, at concentrations found in a cup of green tea, caused increased aggregation of MUC5B in human whole saliva, and increased aggregation and viscosity of purified MUC5B. It was revealed using recombinant proteins of the N- and C-terminal regions of MUC5B that EGCG had these effects by aggregating the terminal globular protein domains of MUC5B. In contrast, MUC5B trypsin-resistant high molecular weight glycopeptides were not aggregated by EGCG, demonstrating that the oligosaccharide-rich, highly-glycosylated regions of mucins are not involved in the EGCG-induced aggregation of mucins. EGCG also caused the majority of MUC7 in human whole saliva to aggregate, and purified MUC7 also showed substantial aggregation in the presence of EGCG.Porcine gastric mucins were also used in order to model human gastric mucins. First, the identity of the porcine gastric mucins was explored using tandem mass spectrometry and immunohistochemistry. This revealed that Muc5ac was expressed by the surface epithelium and was the prominent mucin in porcine gastric mucus. Muc6 was expressed by gastric submucosal glands, but was not a major component of the secreted mucus barrier. Porcine Muc5ac and Muc6 were shown to be aggregated by EGCG. These data demonstrate that mucins from both saliva and the stomach are substantially altered by EGCG. This may contribute to the astringency and low bioavailability of EGCG. In contrast, the green tea polyphenol epicatechin (EC) did not cause aggregation of salivary mucins or porcine gastric mucins, suggesting that the galloyl ring of EGCG (which is absent in EC) is important for its aggregation of mucins, and that EC has different mechanisms of astringency.The structure of the mucins in the alimentary canal was studied using Raman spectroscopy, Raman optical activity (ROA) and Tip-enhanced Raman spectroscopy (TERS). The secondary structure of the oligosaccharide-rich regions of mucins was shown to be largely disordered, with some contribution of poly-proline II helix. The N- and C-terminal regions of MUC5B were largely β-sheet in structure, with some disordered structure also present in the C-terminal region. Raman spectroscopy could reliably distinguish between MUC5B glycoforms, demonstrating the… Advisors/Committee Members: BLANCH, EWAN EW, Thornton, David, Blanch, Ewan.

Subjects/Keywords: Mucin; Stomach; Mucus; Saliva; MUC5B; MUC7; Muc5ac; Muc6; Green tea polyphenols; Epigallocatechin-3-gallate; EGCG; Raman spectroscopy; Raman optical activity; Tip-enhanced Raman spectroscopy

11 more images…

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Davies, H. (2014). Mucins in the alimentary canal: their structure and interactions with polyphenols. (Doctoral Dissertation). University of Manchester. Retrieved from http://www.manchester.ac.uk/escholar/uk-ac-man-scw:239764

Chicago Manual of Style (16^th Edition):

Davies, Heather. “Mucins in the alimentary canal: their structure and interactions with polyphenols.” 2014. Doctoral Dissertation, University of Manchester. Accessed January 15, 2021. http://www.manchester.ac.uk/escholar/uk-ac-man-scw:239764.

MLA Handbook (7^th Edition):

Davies, Heather. “Mucins in the alimentary canal: their structure and interactions with polyphenols.” 2014. Web. 15 Jan 2021.

Vancouver:

Davies H. Mucins in the alimentary canal: their structure and interactions with polyphenols. [Internet] [Doctoral dissertation]. University of Manchester; 2014. [cited 2021 Jan 15]. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:239764.

Council of Science Editors:

Davies H. Mucins in the alimentary canal: their structure and interactions with polyphenols. [Doctoral Dissertation]. University of Manchester; 2014. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:239764

University of Western Ontario

14. McRae, Danielle. Plasmon-Enabled Physical and Chemical Transformations of Nanomaterials.

Degree: 2020, University of Western Ontario

URL: https://ir.lib.uwo.ca/etd/7068

► When the electromagnetic field of light is incident on metallic nanostructures of dimensions smaller than the incident wavelength of the light, there is a strong… (more)

▼ When the electromagnetic field of light is incident on metallic nanostructures of dimensions smaller than the incident wavelength of the light, there is a strong interaction, resulting in an enhanced, highly confined electromagnetic field in the vicinity of the nanostructure. This effect is referred to as a localized surface plasmon resonance, most commonly exploited for plasmon-enhanced spectroscopies, such as surface-enhanced Raman spectroscopy (SERS) and tip-enhanced Raman spectroscopy (TERS). The location, number and intensity of these regions of enhancement, or “hotspots”, can be tuned by changing the nature of the metal, the size, shape and arrangement of the nanoparticles, its surroundings, or the wavelength of the incident light. When a molecule is located within these nanoscale hotspots, it is possible to obtain detailed spectroscopic information about the molecule with high sensitivity. The decay process of these plasmon resonances can result in the ejection of high energy “hot” carriers, either hot electrons or hot holes, and the subsequent heating of the nanoparticle lattice. When a molecule is adsorbed to the surface of the nanoparticle, the presence of hot electrons and holes or the elevation of temperature can favour a chemical reaction. This effect is most prevalent in metallic nanostructures that exhibit hotspots at their surface, that can in turn be used to photocontrol surface reactions through plasmon excitation. In this thesis, plasmon-mediated reactions are investigated using a variety of spectroscopic and microscopic techniques, along with the modelling of the light-matter interaction. The reduction of aryl diazonium salts on a gold nanostructured surface is plasmon-catalyzed. For a tip-enhanced Raman spectroscopy system involving a gold tip and a silver nanoplate as a substrate, plasmonic heating is discussed and modelled using finite element methods. New fractal metallic nanostructures are developed and studied for future applications in plasmon-mediated chemistry.

Subjects/Keywords: plasmonics; tip-enhanced Raman spectroscopy; hot electrons; plasmon-mediated chemistry; finite-difference time-domain; fractal nanostructures; Physical Chemistry

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

McRae, D. (2020). Plasmon-Enabled Physical and Chemical Transformations of Nanomaterials. (Thesis). University of Western Ontario. Retrieved from https://ir.lib.uwo.ca/etd/7068

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

McRae, Danielle. “Plasmon-Enabled Physical and Chemical Transformations of Nanomaterials.” 2020. Thesis, University of Western Ontario. Accessed January 15, 2021. https://ir.lib.uwo.ca/etd/7068.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

McRae, Danielle. “Plasmon-Enabled Physical and Chemical Transformations of Nanomaterials.” 2020. Web. 15 Jan 2021.

Vancouver:

McRae D. Plasmon-Enabled Physical and Chemical Transformations of Nanomaterials. [Internet] [Thesis]. University of Western Ontario; 2020. [cited 2021 Jan 15]. Available from: https://ir.lib.uwo.ca/etd/7068.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

McRae D. Plasmon-Enabled Physical and Chemical Transformations of Nanomaterials. [Thesis]. University of Western Ontario; 2020. Available from: https://ir.lib.uwo.ca/etd/7068

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

15. Heilman, Alexander Lee. Development of a tip-enhanced near-field optical microscope for nanoscale interrogation of surface chemistry and plasmonic phenomena.

Degree: 2017, University of California – eScholarship, University of California

URL: http://www.escholarship.org/uc/item/32m5t332

► Optical microscopy and spectroscopy are invaluable tools for the physical and chemical characterization of materials and surfaces in a wide range of scientific disciplines. However,… (more)

▼ Optical microscopy and spectroscopy are invaluable tools for the physical and chemical characterization of materials and surfaces in a wide range of scientific disciplines. However, the application of conventional optical methods in the study of nanomaterials is inherently limited by diffraction. Tip-enhanced near-field optical microscopy (TENOM) is a hybrid technique that marries optical spectroscopy with scanning probe microscopy to overcome the spatial resolution limit imposed by diffraction. By coupling optical energy into the plasmonic modes of a sharp metal probe tip, a strong, localized optical field is generated near the tip's apex and is used to enhance spectroscopic emissions within a sub-diffraction-limited volume. In this thesis, we describe the design, construction, validation, and application of a custom TENOM instrument with a unique attenuated total reflectance (ATR)-geometry excitation/detection system. The specific goals of this work were: (i) to develop a versatile TENOM instrument capable of investigating a variety of optical phenomena at the nanoscale, (ii) to use the instrument to demonstrate chemical interrogation of surfaces with sub-diffraction-limited spatial resolution (i.e., at super resolution), (iii) to apply the instrument to study plasmonic phenomena that influence spectroscopic enhancement in TENOM measurements, and (iv) to leverage resulting insights to develop systematic improvements that expand the ultimate capabilities of near-field optical interrogation techniques.The TENOM instrument described herein is comprised of three main components: an atomic force microscope (AFM), a side-on confocal Raman microscope, and a novel ATR excitation/detection system. The design of each component is discussed along with the results of relevant validation experiments, which were performed to rigorously assess each component's performance. Finite-difference time-domain (FDTD) optical simulations were also developed and used extensively to evaluate the results of validation studies and to optimize experimental design and instrument performance. By combining and synchronizing the operation of the instrument's three components, we perform a variety of near-field optical experiments that demonstrate the instrument's functionality and versatility. ATR illumination is combined with a plasmonic AFM tip to show that: (i) the tip can quantitatively transduce the optical near-field (evanescent waves) above the surface by scattering photons into the far-field, (ii) the ATR geometry enables excitation and characterization of surface plasmon polaritons (SPPs), whose associated optical fields are shown to enhance Raman scattering from a thin layer of copper phthalocyanine (CuPc), and (iii) SPPs can be used to plasmonically excite the tip for super-resolution chemical imaging of patterned CuPc via tip-enhanced Raman spectroscopy (TERS). ATR-illumination TERS is quantitatively compared with side-on illumination. In both cases, spatial resolution was better than 40 nm and tip-on/tip-off Raman enhancement factors…

Subjects/Keywords: Optics; Nanotechnology; Physics; Atomic force microscopy; Attenuated total reflectance; Near-field optical microscopy; Plasmons; Super-resolution; Tip-enhanced Raman spectroscopy

11 more images…

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Heilman, A. L. (2017). Development of a tip-enhanced near-field optical microscope for nanoscale interrogation of surface chemistry and plasmonic phenomena. (Thesis). University of California – eScholarship, University of California. Retrieved from http://www.escholarship.org/uc/item/32m5t332

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Heilman, Alexander Lee. “Development of a tip-enhanced near-field optical microscope for nanoscale interrogation of surface chemistry and plasmonic phenomena.” 2017. Thesis, University of California – eScholarship, University of California. Accessed January 15, 2021. http://www.escholarship.org/uc/item/32m5t332.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Heilman, Alexander Lee. “Development of a tip-enhanced near-field optical microscope for nanoscale interrogation of surface chemistry and plasmonic phenomena.” 2017. Web. 15 Jan 2021.

Vancouver:

Heilman AL. Development of a tip-enhanced near-field optical microscope for nanoscale interrogation of surface chemistry and plasmonic phenomena. [Internet] [Thesis]. University of California – eScholarship, University of California; 2017. [cited 2021 Jan 15]. Available from: http://www.escholarship.org/uc/item/32m5t332.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Heilman AL. Development of a tip-enhanced near-field optical microscope for nanoscale interrogation of surface chemistry and plasmonic phenomena. [Thesis]. University of California – eScholarship, University of California; 2017. Available from: http://www.escholarship.org/uc/item/32m5t332

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Minnesota

16. Johnson, Timothy. Design, Characterization, and Manufacturing of Ultra-sharp Metallic Probes for Near-field Optics.

Degree: PhD, Electrical Engineering, 2014, University of Minnesota

URL: http://hdl.handle.net/11299/173933

► The ability to manipulate and nanofocus light has many potential applications such as surface-enhanced spectroscopy, optical trapping, heat-assisted magnetic recording, and near-field imaging. In this… (more)

Subjects/Keywords: Nanofocusing; Near-field scanning optical microscope; Plasmonics; Super-resolution imaging; Template stripping; Tip-enhanced Raman scattering

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Johnson, T. (2014). Design, Characterization, and Manufacturing of Ultra-sharp Metallic Probes for Near-field Optics. (Doctoral Dissertation). University of Minnesota. Retrieved from http://hdl.handle.net/11299/173933

Chicago Manual of Style (16^th Edition):

Johnson, Timothy. “Design, Characterization, and Manufacturing of Ultra-sharp Metallic Probes for Near-field Optics.” 2014. Doctoral Dissertation, University of Minnesota. Accessed January 15, 2021. http://hdl.handle.net/11299/173933.

MLA Handbook (7^th Edition):

Johnson, Timothy. “Design, Characterization, and Manufacturing of Ultra-sharp Metallic Probes for Near-field Optics.” 2014. Web. 15 Jan 2021.

Vancouver:

Johnson T. Design, Characterization, and Manufacturing of Ultra-sharp Metallic Probes for Near-field Optics. [Internet] [Doctoral dissertation]. University of Minnesota; 2014. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/11299/173933.

Council of Science Editors:

Johnson T. Design, Characterization, and Manufacturing of Ultra-sharp Metallic Probes for Near-field Optics. [Doctoral Dissertation]. University of Minnesota; 2014. Available from: http://hdl.handle.net/11299/173933

Osaka University

17. Uetsuki, Kazumasa; 植月, 一雅. Plasmon Hybridization for Tip-Enhanced Raman Analysis of Nanomaterials : プラズモンハイブリダイゼーションによるナノマテリアルの先端増強ラマン分析; プラズモンハイブリダイゼーション　ニヨル　ナノマテリアル　ノ　センタン　ゾウキョウ　ラマン　ブンセキ.

Degree: 博士（工学）, 2012, Osaka University

URL: http://hdl.handle.net/11094/24871

博士（工学）

2012-09-25

大阪大学

14401甲第15877号

25627

Subjects/Keywords: plasmon; Raman; graphene; single-walled carbon nanotube; Tip-enhanced Raman scattering; gap

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Uetsuki, Kazumasa; 植月, �. (2012). Plasmon Hybridization for Tip-Enhanced Raman Analysis of Nanomaterials : プラズモンハイブリダイゼーションによるナノマテリアルの先端増強ラマン分析; プラズモンハイブリダイゼーション　ニヨル　ナノマテリアル　ノ　センタン　ゾウキョウ　ラマン　ブンセキ. (Thesis). Osaka University. Retrieved from http://hdl.handle.net/11094/24871

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Uetsuki, Kazumasa; 植月, 一雅. “Plasmon Hybridization for Tip-Enhanced Raman Analysis of Nanomaterials : プラズモンハイブリダイゼーションによるナノマテリアルの先端増強ラマン分析; プラズモンハイブリダイゼーション　ニヨル　ナノマテリアル　ノ　センタン　ゾウキョウ　ラマン　ブンセキ.” 2012. Thesis, Osaka University. Accessed January 15, 2021. http://hdl.handle.net/11094/24871.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Uetsuki, Kazumasa; 植月, 一雅. “Plasmon Hybridization for Tip-Enhanced Raman Analysis of Nanomaterials : プラズモンハイブリダイゼーションによるナノマテリアルの先端増強ラマン分析; プラズモンハイブリダイゼーション　ニヨル　ナノマテリアル　ノ　センタン　ゾウキョウ　ラマン　ブンセキ.” 2012. Web. 15 Jan 2021.

Vancouver:

Uetsuki, Kazumasa; 植月 �. Plasmon Hybridization for Tip-Enhanced Raman Analysis of Nanomaterials : プラズモンハイブリダイゼーションによるナノマテリアルの先端増強ラマン分析; プラズモンハイブリダイゼーション　ニヨル　ナノマテリアル　ノ　センタン　ゾウキョウ　ラマン　ブンセキ. [Internet] [Thesis]. Osaka University; 2012. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/11094/24871.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Uetsuki, Kazumasa; 植月 �. Plasmon Hybridization for Tip-Enhanced Raman Analysis of Nanomaterials : プラズモンハイブリダイゼーションによるナノマテリアルの先端増強ラマン分析; プラズモンハイブリダイゼーション　ニヨル　ナノマテリアル　ノ　センタン　ゾウキョウ　ラマン　ブンセキ. [Thesis]. Osaka University; 2012. Available from: http://hdl.handle.net/11094/24871

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

18. Najjar, Samar. Couplage AFM/Raman et spectroscopie Raman exaltée par effet de pointe de nanostructures : Study of nanostructures with AFM/Raman coupling device and Tip Enhanced Raman Spectroscopy (TERS).

Degree: Docteur es, Chimie-physique, 2013, Université de Bordeaux I

URL: http://www.theses.fr/2013BOR14848

►

Pour mieux comprendre leurs propriétés, diverses nanostructures individuelles ont été étudiées à l’aide d’une technique couplant microscopie à force atomique et spectroscopie Raman confocale. Sous… (more)

▼

Pour mieux comprendre leurs propriétés, diverses nanostructures individuelles ont été étudiées à l’aide d’une technique couplant microscopie à force atomique et spectroscopie Raman confocale. Sous excitation lumineuse polarisée, la composition chimique, la structure et la présence de défauts a pu être précisée dans des nanobâtonnets d’oxydes métalliques (ZnO et α-Fe2O3). Sous irradiation laser résonnante, les spectres de nanotubes de carbone monoparoi enrobés de polymères ont révélé notamment l’absence de transfert de charge polymère-nanotube et un effet de désolvatation. Finalement, des feuillets de graphène oxydé et des ADNs double-brin peignés ont pu être préparés et caractérisés par spectroscopie Raman exaltée par effet de pointe en atteignant une résolution spatiale latérale voisine du rayon de courbure de l’apex de la pointe utilisée (12 nm), bien plus faible que la limite de diffraction, ce qui ouvre la voie à de nouveaux travaux spectroscopiques à l’échelle nanométrique.

To better understand their properties, various nanostructures have been studied using a technique combining atomic force microscopy and confocal Raman spectroscopy. Under polarized light excitation, the chemical composition, the structure and the presence of defects has been described in metal oxides nanorods (ZnO et α-Fe2O3). Under resonant laser excitation, Raman spectra of polymer-wrapped single-walled carbon nanotubes have revealed the absence of polymer-nanotube charge transfer and an effect due to desolvation. Finally, graphene oxide sheets and combed double-stranded DNAs have been prepared and characterized using tip-enhanced Raman spectroscopy with a lateral spatial resolution down to the curvature radius of the apex of the used tip (12 nm), well below the diffraction limit, which opens new opportunities for spectroscopic works at the nanometer scale.

Advisors/Committee Members: Rodriguez, Vincent (thesis director), Bonhommeau, Sébastien (thesis director).

Subjects/Keywords: Spectroscopie Raman exaltée de pointe; Diffusion; Fe2O3 ZnO hématite nanotubes de carbone swnt graphène oxydé adn; Tip Enhanced Raman spectroscopie; Scattering; Fe2O3 ZnO hematite carbon nanotubes swnt graphene oxide dna

…x28;TERS : Tip-Enhanced Raman Spectroscopy) permettant une caractérisation chimique de… …des techniques de spectroscopie optique, et notamment la spectroscopie Raman. Ce chapitre… …savoir la spectroscopie Raman exaltée par effet de pointe tel-00869044, version 1 - 2 Oct 2013… …de spectroscopie Raman. Les aspects plus techniques seront abordés dans le chapitre 2 de ce… …l’état solide, liquide ou gazeux. Parmi elles, la spectroscopie Raman est particulièrement…

12 more images…

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Najjar, S. (2013). Couplage AFM/Raman et spectroscopie Raman exaltée par effet de pointe de nanostructures : Study of nanostructures with AFM/Raman coupling device and Tip Enhanced Raman Spectroscopy (TERS). (Doctoral Dissertation). Université de Bordeaux I. Retrieved from http://www.theses.fr/2013BOR14848

Chicago Manual of Style (16^th Edition):

Najjar, Samar. “Couplage AFM/Raman et spectroscopie Raman exaltée par effet de pointe de nanostructures : Study of nanostructures with AFM/Raman coupling device and Tip Enhanced Raman Spectroscopy (TERS).” 2013. Doctoral Dissertation, Université de Bordeaux I. Accessed January 15, 2021. http://www.theses.fr/2013BOR14848.

MLA Handbook (7^th Edition):

Najjar, Samar. “Couplage AFM/Raman et spectroscopie Raman exaltée par effet de pointe de nanostructures : Study of nanostructures with AFM/Raman coupling device and Tip Enhanced Raman Spectroscopy (TERS).” 2013. Web. 15 Jan 2021.

Vancouver:

Najjar S. Couplage AFM/Raman et spectroscopie Raman exaltée par effet de pointe de nanostructures : Study of nanostructures with AFM/Raman coupling device and Tip Enhanced Raman Spectroscopy (TERS). [Internet] [Doctoral dissertation]. Université de Bordeaux I; 2013. [cited 2021 Jan 15]. Available from: http://www.theses.fr/2013BOR14848.

Council of Science Editors:

Najjar S. Couplage AFM/Raman et spectroscopie Raman exaltée par effet de pointe de nanostructures : Study of nanostructures with AFM/Raman coupling device and Tip Enhanced Raman Spectroscopy (TERS). [Doctoral Dissertation]. Université de Bordeaux I; 2013. Available from: http://www.theses.fr/2013BOR14848

University of Minnesota

19. Chen, Tuo. Detection of pesticide residues on apples using surface-enhanced Raman spectroscopy.

Degree: MS, Food Science, 2014, University of Minnesota

URL: http://hdl.handle.net/11299/165428

► Pesticides are an integral part of agriculture, while increasing use leads to residues in/on agricultural products. Federal monitoring and enforcement action is dependent on the… (more)

▼ Pesticides are an integral part of agriculture, while increasing use leads to residues in/on agricultural products. Federal monitoring and enforcement action is dependent on the technical capability to detect pesticides. However, current methods are elaborate, time-consuming and not cost-effective. In the first part of this work, a rapid and simple surface-enhanced Raman spectroscopy (SERS) method coupled with a surface swab method for recovery and quantitative detection of thiabendazole (TBZ) on apple surfaces was developed, optimized and validated. The whole apple surface was swabbed and the swab was vortexed to release the pesticides. After that, silver dendrites (AgD) were used to bind the pesticide for Raman measurement. The limit of detection of TBZ in methanol was 0.01µg/mL, (10ppb), while the Environmental Protection Agency (EPA) limit is 5µg/gram apple-weight (5 ppm). The concentration of the recovered TBZ was predicted using a partial least square model. The recovery from the surface swab method was calculated to be 59.7% to 76.6% for intentional contamination at 0.1, 0.3, 3 and 5 ppm (µg/g apple-weight) level, respectively. The final accuracy of the swab-SERS method was calculated to be between 90.0% and 115.4%, after corrected by the releasing factor (66.6%). In the second part, a new approach was proposed to detect acetamiprid using an aptamer-based SERS method. The acetamiprid aptamer was chosen from the literature, thoilated and conjugated onto AgD. To block the unbounded surface on the substrate surface after aptamer immobilization, bovine serum albumin (BSA), 2-mercaptoethanol (ME) 6-mercaptohexanol (MCH) and were investigated as blocking agents. MCH and ME cannot fully block the surface when encountered with interference. The typical peaks from acetamiprid did not show on the aptamer-blocking agent-acetampired spectra when using BSA as blocking agents. The aptamer and blocking agent immobility on AgD should be further investigated and the method should be further modified. Last, the swab method was further developed and validated using UV-visible spectroscopy as a reference method. A standard curve was established based on the absorbance at 245 nm at different concentrations from 0 ppm to 1000 ppm. The assay standard curve well fit the five-parameter logistic model (r2 =0.995). The concentration of acetamiprid in the extracts was determined using this standard curve. The recovery rate of refined surface swab method is 90.6 % ± 1.4 % (n=5). This assay has a low intra- and inter-assay coefficient of variation (CV < 5 %).

Subjects/Keywords: Pesticide; Surface-enhanced Raman Spectroscopy

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Chen, T. (2014). Detection of pesticide residues on apples using surface-enhanced Raman spectroscopy. (Masters Thesis). University of Minnesota. Retrieved from http://hdl.handle.net/11299/165428

Chicago Manual of Style (16^th Edition):

Chen, Tuo. “Detection of pesticide residues on apples using surface-enhanced Raman spectroscopy.” 2014. Masters Thesis, University of Minnesota. Accessed January 15, 2021. http://hdl.handle.net/11299/165428.

MLA Handbook (7^th Edition):

Chen, Tuo. “Detection of pesticide residues on apples using surface-enhanced Raman spectroscopy.” 2014. Web. 15 Jan 2021.

Vancouver:

Chen T. Detection of pesticide residues on apples using surface-enhanced Raman spectroscopy. [Internet] [Masters thesis]. University of Minnesota; 2014. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/11299/165428.

Council of Science Editors:

Chen T. Detection of pesticide residues on apples using surface-enhanced Raman spectroscopy. [Masters Thesis]. University of Minnesota; 2014. Available from: http://hdl.handle.net/11299/165428

University of Manchester

20. Mabbott, Samuel. Optimisation of solid-state and solution-based SERS systems for use in the detection of analytes of chemical and biological significance.

Degree: PhD, 2013, University of Manchester

URL: https://www.research.manchester.ac.uk/portal/en/theses/optimisation-of-solidstate-and-solutionbased-sers-systems-for-use-in-the-detection-of-analytes-of-chemical-and-biological-significance(de70094c-8da0-4326-bfb2-6adf00b86af9).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.568608

► Surface enhanced Raman scattering (SERS) has achieved much attention since its conception in 1974. The analytical technique overcomes many difficulties associated with conventional Raman whilst… (more)

▼ Surface enhanced Raman scattering (SERS) has achieved much attention since its conception in 1974. The analytical technique overcomes many difficulties associated with conventional Raman whilst also increasing sensitivity. However, the increased interest and work in the field has also identified flaws, many of which are centred on the irreproducibility of the SERS enhancement effect. The majority of the work described in this thesis focusses on the ‘optimisation’ of solid-state and solution based SERS systems. Optimisation plays a crucial role in maximising both enhancement effects and reproducibility. Here criteria are outlined for the synthesis of high performance solid-state SERS substrates and the synthesis of a range of substrates is assessed, each with associated pros and cons. The most successful substrate was synthesised by exploiting redox potentials which allow for the direct deposition of silver onto copper foil. The deposition times and temperatures were optimised sequentially to generate a high performance substrate capable of detecting Rhodamine 6G at trace levels. Reproducibility comparisons of the silver on copper (SoC) substrate were carried out against commercial substrates: Klarite and QSERS, multiple univariate and multivariate methods were used to assess the substrates performance. The results confirmed that the SoC substrate performed better than both the commercial substrates. The work also highlights the importance of using multiple data analysis methods in order to assess the performance of a solid-state SERS substrate. Deposition of the silver surface was also successful on British 2p coins allowing the for the detection and discrimination of illegal and legal drugs when coupled with multivariate data analysis methods such as PCA and PLS. Solution based SERS analyses were also carried out successfully using different optimisation strategies. The initial investigation involved careful control of the individual components of a SERS system (nanoparticles, aggregating agents and analyte) in order to establish a low limit of detection for the increasingly abused ‘legal high’ MDAI. The use of a reduced factorial design was then successfully employed to explore a greater number of SERS variables and define a low limit of detection for the class B drug mephedrone. The robust experimental design also allowed an insight into the importance of each of the individual components within a solution based SERS system. The final piece of work carried out was the SERS discrimination of antibiotics: ampicillin, ticarcillin and carbenicillin. Optimisation of the solution based experiment allowed the in-situ hydrolysis of the β-lactam moiety present in ampicillin rendering it pharmacologically inactive to be followed under acidic conditions at concentrations of 10 ppm.

Subjects/Keywords: 543; Surface enhanced Raman scattering; Raman spectroscopy

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mabbott, S. (2013). Optimisation of solid-state and solution-based SERS systems for use in the detection of analytes of chemical and biological significance. (Doctoral Dissertation). University of Manchester. Retrieved from https://www.research.manchester.ac.uk/portal/en/theses/optimisation-of-solidstate-and-solutionbased-sers-systems-for-use-in-the-detection-of-analytes-of-chemical-and-biological-significance(de70094c-8da0-4326-bfb2-6adf00b86af9).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.568608

Chicago Manual of Style (16^th Edition):

Mabbott, Samuel. “Optimisation of solid-state and solution-based SERS systems for use in the detection of analytes of chemical and biological significance.” 2013. Doctoral Dissertation, University of Manchester. Accessed January 15, 2021. https://www.research.manchester.ac.uk/portal/en/theses/optimisation-of-solidstate-and-solutionbased-sers-systems-for-use-in-the-detection-of-analytes-of-chemical-and-biological-significance(de70094c-8da0-4326-bfb2-6adf00b86af9).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.568608.

MLA Handbook (7^th Edition):

Mabbott, Samuel. “Optimisation of solid-state and solution-based SERS systems for use in the detection of analytes of chemical and biological significance.” 2013. Web. 15 Jan 2021.

Vancouver:

Mabbott S. Optimisation of solid-state and solution-based SERS systems for use in the detection of analytes of chemical and biological significance. [Internet] [Doctoral dissertation]. University of Manchester; 2013. [cited 2021 Jan 15]. Available from: https://www.research.manchester.ac.uk/portal/en/theses/optimisation-of-solidstate-and-solutionbased-sers-systems-for-use-in-the-detection-of-analytes-of-chemical-and-biological-significance(de70094c-8da0-4326-bfb2-6adf00b86af9).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.568608.

Council of Science Editors:

Mabbott S. Optimisation of solid-state and solution-based SERS systems for use in the detection of analytes of chemical and biological significance. [Doctoral Dissertation]. University of Manchester; 2013. Available from: https://www.research.manchester.ac.uk/portal/en/theses/optimisation-of-solidstate-and-solutionbased-sers-systems-for-use-in-the-detection-of-analytes-of-chemical-and-biological-significance(de70094c-8da0-4326-bfb2-6adf00b86af9).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.568608

21. Kumar, Naresh. Development of Tip-enhanced Raman Spectroscopy and its Application to Heterogeneous Catalysis Research.

Degree: 2018, University Utrecht

URL: https://dspace.library.uu.nl/handle/1874/373428 ; URN:NBN:NL:UI:10-1874-373428 ; 1874/373428 ; urn:isbn:9789493014893 ; URN:NBN:NL:UI:10-1874-373428 ; https://dspace.library.uu.nl/handle/1874/373428

► Catalysis is at the forefront of many developments to make our society more sustainable and less dependent on fossil resources, such as coal, gas and… (more)

▼ Catalysis is at the forefront of many developments to make our society more sustainable and less dependent on fossil resources, such as coal, gas and crude oil. To make this possible, it is important to obtain detailed insights into the working principles of solid catalysts. In the past decades, several powerful analytical techniques have been developed to investigate the working mechanisms of various catalytic materials. Raman spectroscopy is one of these methods, however, it suffers from the limitations of a relatively low sensitivity and poor spatial resolution. A way to counter this is to use tip-enhanced Raman spectroscopy (TERS), which dramatically improves the sensitivity of Raman spectroscopy and pushes its spatial resolution far beyond the optical diffraction limit. This PhD research has focussed on the further development of TERS as a robust and versatile analytical tool for heterogeneous catalysis research. Metallic probes are the very heart of a TERS experiment. Therefore, first, a better understanding into the degradation mechanism of silver-coated TERS probes was gained by conducting a time-series study under different environmental conditions. A significant improvement in the plasmonic lifetime of TERS probes, from a few hours to greater than five months, was obtained under < 1 ppm oxygen and moisture environment. This study allowed practical strategies to be developed for long term preservation of TERS probes. For in situ monitoring of catalytic reactions, TERS has to work in both air and liquid environments. However, current metal coated TERS probes are unsuitable for operation in liquids due to the lack of structural stability. This issue was addressed by developing TERS probes with a unique multi-layer metal coating, which allowed sub-30 nm resolution chemical imaging in a liquid environment using TERS for the first time. To monitor catalytic reactions effectively, TERS probes also need to be chemically inert. For this, we developed a unique method of protecting TERS probes with an ultrathin (1 - 5 nm) zirconia coating, thereby drastically improving their chemical inertness as well as structural stability within a liquid environment. Using these probes, we demonstrated spatially-resolved mapping of a photocatalytic reaction over a heterogeneous catalyst substrate within a liquid environment for the first time. Finally, we introduced tip-enhanced fluorescence (TEFL) microscopy as a new tool for heterogeneous catalysis research. Using selective staining of Brønsted acidity in single fluid cracking catalyst (FCC) particles we performed high‑resolution TEFL mapping of different regions, which revealed a hierarchical distribution of Brønsted acidity within individual zeolite domains. Comparison of TEFL measurements from different FCC particles showed significant intra- and inter-particle heterogeneities in zeolite domain size and activity. With a dramatic improvement in the lifetime and structural and chemical stability of Ag coated probes, this research has brought TERS a step closer to becoming a… Advisors/Committee Members: Weckhuysen, Bert.

Subjects/Keywords: Heterogenous catalysis; Tip-enhanced Raman spectroscopy; TERS probes; Nanoscale chemical imaging; Tip-enhanced fluorescence microscopy; Fluid cracking catalyst particles

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kumar, N. (2018). Development of Tip-enhanced Raman Spectroscopy and its Application to Heterogeneous Catalysis Research. (Doctoral Dissertation). University Utrecht. Retrieved from https://dspace.library.uu.nl/handle/1874/373428 ; URN:NBN:NL:UI:10-1874-373428 ; 1874/373428 ; urn:isbn:9789493014893 ; URN:NBN:NL:UI:10-1874-373428 ; https://dspace.library.uu.nl/handle/1874/373428

Chicago Manual of Style (16^th Edition):

Kumar, Naresh. “Development of Tip-enhanced Raman Spectroscopy and its Application to Heterogeneous Catalysis Research.” 2018. Doctoral Dissertation, University Utrecht. Accessed January 15, 2021. https://dspace.library.uu.nl/handle/1874/373428 ; URN:NBN:NL:UI:10-1874-373428 ; 1874/373428 ; urn:isbn:9789493014893 ; URN:NBN:NL:UI:10-1874-373428 ; https://dspace.library.uu.nl/handle/1874/373428.

MLA Handbook (7^th Edition):

Kumar, Naresh. “Development of Tip-enhanced Raman Spectroscopy and its Application to Heterogeneous Catalysis Research.” 2018. Web. 15 Jan 2021.

Vancouver:

Kumar N. Development of Tip-enhanced Raman Spectroscopy and its Application to Heterogeneous Catalysis Research. [Internet] [Doctoral dissertation]. University Utrecht; 2018. [cited 2021 Jan 15]. Available from: https://dspace.library.uu.nl/handle/1874/373428 ; URN:NBN:NL:UI:10-1874-373428 ; 1874/373428 ; urn:isbn:9789493014893 ; URN:NBN:NL:UI:10-1874-373428 ; https://dspace.library.uu.nl/handle/1874/373428.

Council of Science Editors:

Kumar N. Development of Tip-enhanced Raman Spectroscopy and its Application to Heterogeneous Catalysis Research. [Doctoral Dissertation]. University Utrecht; 2018. Available from: https://dspace.library.uu.nl/handle/1874/373428 ; URN:NBN:NL:UI:10-1874-373428 ; 1874/373428 ; urn:isbn:9789493014893 ; URN:NBN:NL:UI:10-1874-373428 ; https://dspace.library.uu.nl/handle/1874/373428

Kyoto University / 京都大学

22. Itasaka, Hiroki. Area-selective electroless deposition of gold nanostructures on silicon : シリコン表面での局所選択的無電解金ナノ構造成長.

Degree: 博士(工学), 2016, Kyoto University / 京都大学

URL: http://hdl.handle.net/2433/215550 ; http://dx.doi.org/10.14989/doctor.k19724

新制・課程博士

甲第19724号

工博第4179号

Subjects/Keywords: silicon; gold nanostructure; electroless deposition; focused ion beam; tip-enhanced Raman spectroscopy

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Itasaka, H. (2016). Area-selective electroless deposition of gold nanostructures on silicon : シリコン表面での局所選択的無電解金ナノ構造成長. (Thesis). Kyoto University / 京都大学. Retrieved from http://hdl.handle.net/2433/215550 ; http://dx.doi.org/10.14989/doctor.k19724

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Itasaka, Hiroki. “Area-selective electroless deposition of gold nanostructures on silicon : シリコン表面での局所選択的無電解金ナノ構造成長.” 2016. Thesis, Kyoto University / 京都大学. Accessed January 15, 2021. http://hdl.handle.net/2433/215550 ; http://dx.doi.org/10.14989/doctor.k19724.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Itasaka, Hiroki. “Area-selective electroless deposition of gold nanostructures on silicon : シリコン表面での局所選択的無電解金ナノ構造成長.” 2016. Web. 15 Jan 2021.

Vancouver:

Itasaka H. Area-selective electroless deposition of gold nanostructures on silicon : シリコン表面での局所選択的無電解金ナノ構造成長. [Internet] [Thesis]. Kyoto University / 京都大学; 2016. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/2433/215550 ; http://dx.doi.org/10.14989/doctor.k19724.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Itasaka H. Area-selective electroless deposition of gold nanostructures on silicon : シリコン表面での局所選択的無電解金ナノ構造成長. [Thesis]. Kyoto University / 京都大学; 2016. Available from: http://hdl.handle.net/2433/215550 ; http://dx.doi.org/10.14989/doctor.k19724

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Western Ontario

23. Pashaee, Farshid. Vibrational Imaging at the Nanoscale: Surpassing the Diffraction Limit Using Tip-Enhanced Raman Spectroscopy.

Degree: 2015, University of Western Ontario

URL: https://ir.lib.uwo.ca/etd/3426

► A deep understanding of the chemical composition of surfaces, interfaces or nanoscale structure with a high spatial resolution is an important goal in nanoscience and… (more)

▼ A deep understanding of the chemical composition of surfaces, interfaces or nanoscale structure with a high spatial resolution is an important goal in nanoscience and nanotechnology. Structural information can be collected using a variety of high spatial resolution techniques such as atomic force microscopy (AFM), scanning tunneling microscopy (STM), scanning electron microscopy (SEM), or transmission electron microscopy (TEM). Nevertheless, these methods do not offer molecular information such as vibrational spectroscopy techniques that allow one to collect molecular or lattice vibrations yielding to a precise picture of the molecular interactions in bulk materials as well as in surfaces and interfaces. Unfortunately optical spectroscopy techniques are limited in terms of spatial resolution and sensitivity due to the poor signal/noise ratio of the localized measurement. Surface- and tip-enhanced Raman spectroscopy (SERS and TERS) are advanced spectroscopic techniques, which are becoming widely used and show a great potential for the structural characterisation of biological systems. Surface-enhanced spectroscopy (SERS) was developed to improve the sensitivity of the chemical measurements by using rough silver or gold surfaces. The challenge of the simultaneous improvement of the spatial resolution and sensitivity was addressed by combining high resolution optical microscopy with the high sensitivity of surface-enhanced spectroscopy and was termed tip-enhanced Raman spectroscopy (TERS). In this thesis, gap-mode TERS is developed for the study of a variety of materials. TERS is used in conjunction with gold nanoplates to serve as an ultraflat substrate that can possibly be functionalized. TERS investigation of monolayers adsorbed onto gold nanoplates such as alkoxy substituted azobenzene thiol and 4-nitrothiophenol is conducted. The monolayer is probed with a silver coated AFM tip in order to obtain the largest electromagnetic field enhancement and the effect of the excitation (linearly or radially polarized) is conducted. TERS is also used to probe graphene flakes and differentiate the edges of a few-layer graphene flakes with a spatial resolution better than 20 nm. Last, TERS was used to investigate single DNA molecules deposited onto gold nanoplates. The DNA, cDNA and pure plasmid were investigated with TERS probing the distribution of nucleobases at a specific location with a spatial resolution which was, in the best conditions below 10 nm.

Subjects/Keywords: Tip-enhanced Raman spectroscopy; atomic force microscopy; localized surface plasmon resonances; near-field measurements; spatial resolution; radial and linear polarization; Analytical Chemistry; Materials Chemistry; Physical Chemistry

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Pashaee, F. (2015). Vibrational Imaging at the Nanoscale: Surpassing the Diffraction Limit Using Tip-Enhanced Raman Spectroscopy. (Thesis). University of Western Ontario. Retrieved from https://ir.lib.uwo.ca/etd/3426

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Pashaee, Farshid. “Vibrational Imaging at the Nanoscale: Surpassing the Diffraction Limit Using Tip-Enhanced Raman Spectroscopy.” 2015. Thesis, University of Western Ontario. Accessed January 15, 2021. https://ir.lib.uwo.ca/etd/3426.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Pashaee, Farshid. “Vibrational Imaging at the Nanoscale: Surpassing the Diffraction Limit Using Tip-Enhanced Raman Spectroscopy.” 2015. Web. 15 Jan 2021.

Vancouver:

Pashaee F. Vibrational Imaging at the Nanoscale: Surpassing the Diffraction Limit Using Tip-Enhanced Raman Spectroscopy. [Internet] [Thesis]. University of Western Ontario; 2015. [cited 2021 Jan 15]. Available from: https://ir.lib.uwo.ca/etd/3426.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Pashaee F. Vibrational Imaging at the Nanoscale: Surpassing the Diffraction Limit Using Tip-Enhanced Raman Spectroscopy. [Thesis]. University of Western Ontario; 2015. Available from: https://ir.lib.uwo.ca/etd/3426

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Kyoto University

24. Itasaka, Hiroki. Area-selective electroless deposition of gold nanostructures on silicon .

Degree: 2016, Kyoto University

URL: http://hdl.handle.net/2433/215550

Subjects/Keywords: silicon; gold nanostructure; electroless deposition; focused ion beam; tip-enhanced Raman spectroscopy

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Itasaka, H. (2016). Area-selective electroless deposition of gold nanostructures on silicon . (Thesis). Kyoto University. Retrieved from http://hdl.handle.net/2433/215550

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Itasaka, Hiroki. “Area-selective electroless deposition of gold nanostructures on silicon .” 2016. Thesis, Kyoto University. Accessed January 15, 2021. http://hdl.handle.net/2433/215550.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Itasaka, Hiroki. “Area-selective electroless deposition of gold nanostructures on silicon .” 2016. Web. 15 Jan 2021.

Vancouver:

Itasaka H. Area-selective electroless deposition of gold nanostructures on silicon . [Internet] [Thesis]. Kyoto University; 2016. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/2433/215550.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Itasaka H. Area-selective electroless deposition of gold nanostructures on silicon . [Thesis]. Kyoto University; 2016. Available from: http://hdl.handle.net/2433/215550

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

25. Akanny, Elie. Développement d’une méthode d’analyse de bactéries par Spectroscopie Raman Exaltée de Surface : application à la caractérisation de probiotiques microencapsulés pour le ciblage colique : Development of a method for bacterial analysis by Surface Enhanced Raman Spectroscopy : application to the characterization of microencapsulated probiotics for colonic targeting.

Degree: Docteur es, Chimie, 2019, Lyon

URL: http://www.theses.fr/2019LYSE1277

►

L’administration orale de probiotiques en vue d’un ciblage colique fait face à un problème majeur lié aux conditions délétères à leur survie rencontrées tout au… (more)

▼

L’administration orale de probiotiques en vue d’un ciblage colique fait face à un problème majeur lié aux conditions délétères à leur survie rencontrées tout au long de leur parcours dans le tractus gastro-intestinal. En effet, il s’avère que la quantité de microorganismes viables arrivant au colon peu souvent être insuffisante pour apporter l’effet bénéfique souhaité, c’est-à-dire un maintien ou une restauration des fonctions du microbiote intestinal. Pour lever ce verrou thérapeutique, la technologie de la microencapsulation a été envisagée. L’un des objectifs de ce travail de thèse a donc été de développer un système d’encapsulation permettant d’assurer le ciblage colique, après administration par voie orale, du Lactobacillus rhamanosus GG (LGG), souche probiotique indiquée dans le traitement et la prévention de la diarrhée associée aux antibiotiques. Le développement d’un tel système implique l’emploi d’une technique de quantification des microorganismes afin de déterminer par exemple le rendement d’encapsulation ou encore la cinétique de libération des bactéries. Pour cela, la technique classique de dénombrement sur milieu solide est généralement employée mais apparait comme longue et fastidieuse. Du fait de sa rapidité et simplicité, la Spectroscopie Raman Exaltée de Surface (SERS), permettant l’augmentation importante du signal Raman conventionnel de molécules adsorbées sur des surfaces métalliques nanostructurées de métaux nobles (argent, or), a été investiguée en tant que technique alternative. Une méthode SERS de quantification des LGG a donc été développée et appliquée pour caractériser le système d’encapsulation formulé. Les microparticules encapsulant les LGG ont été obtenues par spray-drying d’une solution aqueuse d’un polymère entérique contenant un agent de protection des bactéries des effets délétères du séchage. Un bon maintien de la viabilité des microorganismes a donc pu être observé au cours du procédé de spray-drying. La protection des LGG en conditions gastro-intestinales simulées, assurée par la matrice d’encapsulation, atteste de l’obtention d’un système d’encapsulation adapté au ciblage colique de LGG viables en nombre suffisant pour potentiellement assurer l’efficacité du traitement probiotique après administration par voie orale

For their specific colonic delivery after oral administration, probiotics have to deal with the deleterious conditions of the gastro-intestinal tract that could alter their viability or functionality. Indeed, the quantity of viable microorganisms reaching the colon is often insufficient to provide the desired beneficial effect, namely, to maintain or restore the functions of the intestinal microbiota. To resolve this problem, the microencapsulation technology was considered. One aim of this project was thus to develop an encapsulation system for the colonic delivery via the oral route of the Lactobacillus rhamanosus GG (LGG), indicated for the treatment and the prevention of antibiotic-associated diarrhea. The development of such system requires a reliable…

Advisors/Committee Members: Bordes, Claire (thesis director), Bourgeois, Sandrine (thesis director), Bessueille, François (thesis director).

Subjects/Keywords: Probiotiques; Lactobacillus Rhamnosus GG; Microparticules; Ciblage colique; Microencapsulation; Spectroscopie Raman Exaltée de Surface; Nanoparticules d'or; Rhodamine 6G; Probiotics; Lactobacillus Rhamnosus GG; Microparticles; Colonic delivery; Microencapsulation; Surface Enhanced Raman Spectroscopy; Gold nanoparticles; Rhodamine 6G; 540

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Akanny, E. (2019). Développement d’une méthode d’analyse de bactéries par Spectroscopie Raman Exaltée de Surface : application à la caractérisation de probiotiques microencapsulés pour le ciblage colique : Development of a method for bacterial analysis by Surface Enhanced Raman Spectroscopy : application to the characterization of microencapsulated probiotics for colonic targeting. (Doctoral Dissertation). Lyon. Retrieved from http://www.theses.fr/2019LYSE1277

Chicago Manual of Style (16^th Edition):

Akanny, Elie. “Développement d’une méthode d’analyse de bactéries par Spectroscopie Raman Exaltée de Surface : application à la caractérisation de probiotiques microencapsulés pour le ciblage colique : Development of a method for bacterial analysis by Surface Enhanced Raman Spectroscopy : application to the characterization of microencapsulated probiotics for colonic targeting.” 2019. Doctoral Dissertation, Lyon. Accessed January 15, 2021. http://www.theses.fr/2019LYSE1277.

MLA Handbook (7^th Edition):

Akanny, Elie. “Développement d’une méthode d’analyse de bactéries par Spectroscopie Raman Exaltée de Surface : application à la caractérisation de probiotiques microencapsulés pour le ciblage colique : Development of a method for bacterial analysis by Surface Enhanced Raman Spectroscopy : application to the characterization of microencapsulated probiotics for colonic targeting.” 2019. Web. 15 Jan 2021.

Vancouver:

Akanny E. Développement d’une méthode d’analyse de bactéries par Spectroscopie Raman Exaltée de Surface : application à la caractérisation de probiotiques microencapsulés pour le ciblage colique : Development of a method for bacterial analysis by Surface Enhanced Raman Spectroscopy : application to the characterization of microencapsulated probiotics for colonic targeting. [Internet] [Doctoral dissertation]. Lyon; 2019. [cited 2021 Jan 15]. Available from: http://www.theses.fr/2019LYSE1277.

Council of Science Editors:

Akanny E. Développement d’une méthode d’analyse de bactéries par Spectroscopie Raman Exaltée de Surface : application à la caractérisation de probiotiques microencapsulés pour le ciblage colique : Development of a method for bacterial analysis by Surface Enhanced Raman Spectroscopy : application to the characterization of microencapsulated probiotics for colonic targeting. [Doctoral Dissertation]. Lyon; 2019. Available from: http://www.theses.fr/2019LYSE1277

26. Zhou, Xuan. Advances in hybrid plasmonics : from passive to active functions : Nouvelles avancées en nanoplasmonique hybride : intégration de fonctions passives et actives.

Degree: Docteur es, Optique et Nanotechnologies, 2013, Troyes

URL: http://www.theses.fr/2013TROY0015

►

La plasmonique hybride est un sujet d’actualité qui exploite des interactions physiques entre nano-objets métalliques et d’autres nanomatériaux. En bénéficiant des propriétés de chacun de… (more)

▼

La plasmonique hybride est un sujet d’actualité qui exploite des interactions physiques entre nano-objets métalliques et d’autres nanomatériaux. En bénéficiant des propriétés de chacun de leurs constituants, les nanostructures hybrides sont utilisées dans de nombreuses applications comme la détection d’espèces bio-chimiques. Dans cette thèse, nous présentons une nouvelle nanostructure hybride polymère/metal qui est non seulement utilisée comme nano-émetteur anisotrope qui s’avère aussi être un outil puissant de caractérisation du champ proche optique.La fabrication de cette nouvelle nanostructure est basée sur une approche de par photopolymérisation à l’échelle nanométrique. Cette technique, en comparaison aux méthodes traditionnelles de caractérisation, ne fournit pas seulement l’image de la distribution du champ, mais permet aussi des mesures quantitatives des plasmons de surface avec une résolution sub -5nm, incluant une description fine de la décroissance exponentielle des ondes évanescentes impliquées.A l’aide du mode plasmon dipolaire, une distribution anisotrope de matériau organique est intégrée dans le voisinage de la nanoparticule métallique. Avec une haute concentration de molécules de colorant dans le polymère, l’intensité des signaux de fluorescence et Raman du nano-émetteur hybride dépend de la polarisation incidente. À notre connaissance, il s’agit de la première réalisation d’un nano-émetteur dont le milieu à gain présente une distribution spatiale complexe le rendant sensible à la polarisation

Hybrid plasmonics has given rise to increasing interest in the context of the interaction between metal nano-objects and other materials. By benefiting from each of its constituents, hybrid nanostructures are commonly adopted in studies and optimization of biological and chemical sensors, nanoparticle with high plasmon resonance tunability, and nano-emitters. This PhD thesis presents a hybrid nanostructure of photopolymer/metal nanoparticle that is used as a near-field characterizing tool and as an anisotropic nano-emitter.The fabrication of this hybrid nanostructure is a near-field imprinting process based on nanoscale photopolymerization. This technique, compared with traditional near-field characterization methods, provides not only the image of the field distribution, but also enables quantification of the surface plasmon properties with sub-5nm resolution and reproduction of the exponential decay of the near-field.Under dipolar mode plasmon, the photopolymer was created anisotropically in the vicinity of the metal nanoparticle. With high concentration of dye molecules trapped in the polymer, the hybrid nano-emitter displays surface enhanced fluorescence and Raman signal that is dependent on the incident polarization. To our knowledge, this is the first achievement of the anisotropic nano-emitter based on the inhomogeneous distribution of the active molecule

Advisors/Committee Members: Bachelot, Renaud (thesis director).

Subjects/Keywords: Optique en champ proche; Plasmons; Spectroscopie de fluorescence; Raman, effet augmenté de surface; Matériaux nanostructurés; Polymérisation; Near-field optics; Plasmons (physics); Fluorescence spectroscopy; Raman effect, surface enhanced; Nanostructural materials; Polymerization; 620.5

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Zhou, X. (2013). Advances in hybrid plasmonics : from passive to active functions : Nouvelles avancées en nanoplasmonique hybride : intégration de fonctions passives et actives. (Doctoral Dissertation). Troyes. Retrieved from http://www.theses.fr/2013TROY0015

Chicago Manual of Style (16^th Edition):

Zhou, Xuan. “Advances in hybrid plasmonics : from passive to active functions : Nouvelles avancées en nanoplasmonique hybride : intégration de fonctions passives et actives.” 2013. Doctoral Dissertation, Troyes. Accessed January 15, 2021. http://www.theses.fr/2013TROY0015.

MLA Handbook (7^th Edition):

Zhou, Xuan. “Advances in hybrid plasmonics : from passive to active functions : Nouvelles avancées en nanoplasmonique hybride : intégration de fonctions passives et actives.” 2013. Web. 15 Jan 2021.

Vancouver:

Zhou X. Advances in hybrid plasmonics : from passive to active functions : Nouvelles avancées en nanoplasmonique hybride : intégration de fonctions passives et actives. [Internet] [Doctoral dissertation]. Troyes; 2013. [cited 2021 Jan 15]. Available from: http://www.theses.fr/2013TROY0015.

Council of Science Editors:

Zhou X. Advances in hybrid plasmonics : from passive to active functions : Nouvelles avancées en nanoplasmonique hybride : intégration de fonctions passives et actives. [Doctoral Dissertation]. Troyes; 2013. Available from: http://www.theses.fr/2013TROY0015

Brno University of Technology

27. Gallina, Pavel. Využití kovové sondy pro ovládání optických procesů a zobrazování v blízkém poli: Applications of metallic probe for the control of optical processes and near-field imaging.

Degree: 2018, Brno University of Technology

URL: http://hdl.handle.net/11012/83374

► The main subject of this master’s thesis are electromagnetic simulations using the finite element method (FEM) to investigate the influence of graphene on tip-enhanced Raman… (more)

Subjects/Keywords: povrchové plasmonové polaritony; plasmonika; hrotem zesílená Ramanova spektroskopie; grafenem zesílená Ramanova spektroskopie; grafen; povrchem zesílená infračervená absorpční spektroskopie; skenovací optický mikroskop blízkého pole; citlivost SNOM hrotu; metoda konečných prvků; surface plasmon polaritons (SPP); plasmonics; tip-enhanced Raman spectroscopy (TERS); graphene-enhanced Raman spectroscopy (GERS); graphene; surface-enhanced infrared absorption spectroscopy (SEIRA); scanning near-field optical microscopy (SNOM); SNOM tip sensitivity; fineite element method (FEM)

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Gallina, P. (2018). Využití kovové sondy pro ovládání optických procesů a zobrazování v blízkém poli: Applications of metallic probe for the control of optical processes and near-field imaging. (Thesis). Brno University of Technology. Retrieved from http://hdl.handle.net/11012/83374

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Gallina, Pavel. “Využití kovové sondy pro ovládání optických procesů a zobrazování v blízkém poli: Applications of metallic probe for the control of optical processes and near-field imaging.” 2018. Thesis, Brno University of Technology. Accessed January 15, 2021. http://hdl.handle.net/11012/83374.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Gallina, Pavel. “Využití kovové sondy pro ovládání optických procesů a zobrazování v blízkém poli: Applications of metallic probe for the control of optical processes and near-field imaging.” 2018. Web. 15 Jan 2021.

Vancouver:

Gallina P. Využití kovové sondy pro ovládání optických procesů a zobrazování v blízkém poli: Applications of metallic probe for the control of optical processes and near-field imaging. [Internet] [Thesis]. Brno University of Technology; 2018. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/11012/83374.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Gallina P. Využití kovové sondy pro ovládání optických procesů a zobrazování v blízkém poli: Applications of metallic probe for the control of optical processes and near-field imaging. [Thesis]. Brno University of Technology; 2018. Available from: http://hdl.handle.net/11012/83374

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

28. Figueroa, Manuel Alejandro. Fabrication of surface enhanced Raman Scattering (SERS) substrates made from nanoparticle printing inks for detection of biological molecules.

Degree: 2012, Drexel University

URL: http://hdl.handle.net/1860/3975

►

Surface enhanced Raman scattering (SERS) has generated great interest as a surface analytical technique because it can produce amplification factors between 108-1012. Silver and gold… (more)

▼

Surface enhanced Raman scattering (SERS) has generated great interest as a surface analytical technique because it can produce amplification factors between 108-1012. Silver and gold are the most widely used components as their size and structure allows for light to induce conduction electrons to oscillate locally within the nanoparticle structure. When a molecule lies in the interparticle space between two nanoparticles, highly detailed vibrational information becomes detectable. The objective of this study is to reproducibly fabricate such an arrangement in a nanoparticle substrate while maintaining stability.In this work, nanoparticle printing inks – colloidal nanoparticles encapsulated by a stabilizing ligand – are used as the main component of SERS substrates. The ligand shell is partially removed by controlled heating, which reduces spacing between nanoparticles creating a broad distribution of interparticle distances. Similar to fractal aggregates this arrangement allows localized plasmons to naturally resonate over a broad range of spectral frequencies.Microwave absorption is applied as a non-invasive method to sensitively monitor nanoparticle sintering in order to gauge the substrates’ tuning for large amplification factors. The global arrangement of nanoparticles has always been difficult to measure during heating through DC resistivity measurements and surface imaging techniques. Microwave absorption occurs in the weak resistive links formed between particles during sintering due to the microwave losses in loosely coupled particles. By placing the substrate in a microwave cavity, absorption can be monitored globally during heating. The largest SERS amplification factors occur at a stage immediately preceding the largest microwave absorption gains. This provides a useful method for determining a thermal window for heating when optimizing SERS substrates.Finally, these optimized SERS substrates are used to detect hyaluronic acid. This complex molecule is a potential biomarker for inflammatory diseases but it has only been detected at a concentration of 5 mg/mL on commercially available SERS substrates. Here it is shown that functionalizing the SERS substrate with a self-assembled monolayer lowers the limit of detection to 50 μg/mL. SERS analysis also provides structural details about the conformation of the molecule during adsorption.

Ph.D., Biomedical Engineering – Drexel University, 2012

Advisors/Committee Members: Tyagi, Som, Pourrezaei, Kambiz.

Subjects/Keywords: Biomedical engineering; Raman effect, Surface enhanced; Nanoparticles

10 more images…

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Figueroa, M. A. (2012). Fabrication of surface enhanced Raman Scattering (SERS) substrates made from nanoparticle printing inks for detection of biological molecules. (Thesis). Drexel University. Retrieved from http://hdl.handle.net/1860/3975

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Figueroa, Manuel Alejandro. “Fabrication of surface enhanced Raman Scattering (SERS) substrates made from nanoparticle printing inks for detection of biological molecules.” 2012. Thesis, Drexel University. Accessed January 15, 2021. http://hdl.handle.net/1860/3975.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Figueroa, Manuel Alejandro. “Fabrication of surface enhanced Raman Scattering (SERS) substrates made from nanoparticle printing inks for detection of biological molecules.” 2012. Web. 15 Jan 2021.

Vancouver:

Figueroa MA. Fabrication of surface enhanced Raman Scattering (SERS) substrates made from nanoparticle printing inks for detection of biological molecules. [Internet] [Thesis]. Drexel University; 2012. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/1860/3975.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Figueroa MA. Fabrication of surface enhanced Raman Scattering (SERS) substrates made from nanoparticle printing inks for detection of biological molecules. [Thesis]. Drexel University; 2012. Available from: http://hdl.handle.net/1860/3975

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Rice University

29. Hughes, Hannah Jacqueline. Structural Analysis of Lipid Membrane Probes by Enhanced Raman Scattering.

Degree: PhD, Physics, 2020, Rice University

URL: http://hdl.handle.net/1911/109214

► Fluorescent membrane probes are one of the many tools used to study the complex structure and processes of cell membranes. The detailed molecular positions and… (more)

▼ Fluorescent membrane probes are one of the many tools used to study the complex structure and processes of cell membranes. The detailed molecular positions and orientations of membrane fluorophores are not well known. These parameters are of interest because they could influence how the fluorescent signal is interpreted. Furthermore, some membrane probes behave differently in different membrane systems, motivating the need to understand how the lipid environment affects the probes orientation and signal. Surface enhanced Raman spectroscopy was used to study the molecular positions and orientations of fluorescent membrane probes embedded in a lipid bilayer in a natural fluid environment. Gold nanostructures can act as an antenna, focusing and enhancing electromagnetic radiation at their surface. When gold nanorods are excited by electromagnetic radiation at their plasmon resonant frequency, the nanoparticle’s free electron gas oscillates, leading to surface enhanced Raman spectroscopy (SERS). SERS a distance dependent enhancement of the Raman scattering signal the allows the possibility to visualize molecular structure. This project utilizes gold nanorods, tuned to an excitation laser wavelength of 785 nm, as a substrate for lipid membranes so that their structure can be analyzed by SERS. As synthesized, gold nanorods are stabilized by the surfactant CTAB. The rods are resuspended in a solution containing a 9:1 molar ratio of DOPC and DOPG, which replace the CTAB to form a well ordered, fluid bilayer electrostatically bound to the gold nanorod surface. Once a lipid bilayer was exchanged onto the gold nanorod surface, a lipid solution interspersed with fluorescent membrane probes was exchanged onto the nanorod surface. The presence of fluorescent probes in the bilayer was confirmed by identifying peak corresponding to the probes in the SERS spectra. The structural analysis by enhanced Raman scattering (SABERS) method combines SERS and unenhanced Raman spectra with theoretical calculations of the optical field and molecular polarizability. Raman measurements of the samples are orientationally averaged, while SERS spectra contain information on molecular position and orientation relative to the surface. Together these reveal the molecular orientation and position of fluorescent membrane probes in phospholipid bilayers. This method offers an approach to analyzing lipid membrane molecular structure under ambient conditions, with microscopic quantities, and without molecular labels. Advisors/Committee Members: Hafner, Jason (advisor).

Subjects/Keywords: surface enhanced Raman spectroscopy (SERS); plasmonics

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hughes, H. J. (2020). Structural Analysis of Lipid Membrane Probes by Enhanced Raman Scattering. (Doctoral Dissertation). Rice University. Retrieved from http://hdl.handle.net/1911/109214

Chicago Manual of Style (16^th Edition):

Hughes, Hannah Jacqueline. “Structural Analysis of Lipid Membrane Probes by Enhanced Raman Scattering.” 2020. Doctoral Dissertation, Rice University. Accessed January 15, 2021. http://hdl.handle.net/1911/109214.

MLA Handbook (7^th Edition):

Hughes, Hannah Jacqueline. “Structural Analysis of Lipid Membrane Probes by Enhanced Raman Scattering.” 2020. Web. 15 Jan 2021.

Vancouver:

Hughes HJ. Structural Analysis of Lipid Membrane Probes by Enhanced Raman Scattering. [Internet] [Doctoral dissertation]. Rice University; 2020. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/1911/109214.

Council of Science Editors:

Hughes HJ. Structural Analysis of Lipid Membrane Probes by Enhanced Raman Scattering. [Doctoral Dissertation]. Rice University; 2020. Available from: http://hdl.handle.net/1911/109214

Boston University

30. Yan, Bo. Rationally designed substrates for SERS biosensing.

Degree: PhD, Chemistry, 2013, Boston University

URL: http://hdl.handle.net/2144/12894

► The large electromagnetic field enhancement provided by nanostructured noble metal surfaces forms the foundation for a series of enabling optical analytical techniques, such as surface… (more)

▼ The large electromagnetic field enhancement provided by nanostructured noble metal surfaces forms the foundation for a series of enabling optical analytical techniques, such as surface enhanced Raman spectroscopy (SERS), surface enhanced IR absorption spectroscopy (SEIRA), surface enhanced fluorescent microscopy (SEF), to name only a few. Critical sensing applications have, however, other substrate requirements than mere peak signal enhancement. The substrate needs to be reliable, provide reproducible signal enhancements, and be amenable to a combination with microfluidic chips or other integrated sensor platforms. These needs motivate the development of engineerable SERS substrate "chips" with defined near- and far-field responses. In this dissertation, two types of rationally designed SERS substrates - nanoparticle cluster arrays (NCAs) and SERS stamp - will be introduced and characterized. NCAs were fabricated through a newly developed template guided self-assembly fabrication approach, in which chemically synthesized nanoparticles are integrated into predefined patterns using a hybrid top-down/bottom-up approach. Since this method relies on chemically defined building blocks, it can overcome the resolution limit of conventional lithographical methods and facilitates higher structural complexity. NCAs sustain near-field interactions within individual clusters as well as between entire neighboring clusters and create a multi-scale cascaded E-field enhancement throughout the entire array. SERS stamps were generated using an oblique angle metal deposition on a lithographically defined piston. When mounted on a nanopositioning stage, the SERS stamps were enabled to contact biological surfaces with pristine nanostructured metal surfaces for a label-free spectroscopic characterization. The developed engineered substrates were applied and tested in critical sensing applications, including the ultratrace detection of explosive vapors, the rapid discrimination of bacterial pathogens, and the label-free monitoring of the enzymatic degradation of pericellular matrices of cancer cells.

Subjects/Keywords: Surface-enhanced Raman scattering; Biosensing applications

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Yan, B. (2013). Rationally designed substrates for SERS biosensing. (Doctoral Dissertation). Boston University. Retrieved from http://hdl.handle.net/2144/12894

Chicago Manual of Style (16^th Edition):

Yan, Bo. “Rationally designed substrates for SERS biosensing.” 2013. Doctoral Dissertation, Boston University. Accessed January 15, 2021. http://hdl.handle.net/2144/12894.

MLA Handbook (7^th Edition):

Yan, Bo. “Rationally designed substrates for SERS biosensing.” 2013. Web. 15 Jan 2021.

Vancouver:

Yan B. Rationally designed substrates for SERS biosensing. [Internet] [Doctoral dissertation]. Boston University; 2013. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/2144/12894.

Council of Science Editors:

Yan B. Rationally designed substrates for SERS biosensing. [Doctoral Dissertation]. Boston University; 2013. Available from: http://hdl.handle.net/2144/12894

		in
And / Or
		in
And / Or
		in
And / Or
		in

Open Access Theses and Dissertations