Advanced search options

Advanced Search Options 🞨

Browse by author name (“Author name starts with…”).

Find ETDs with:

in
/  
in
/  
in
/  
in

Written in Published in Earliest date Latest date

Sorted by

Results per page:

Sorted by: relevance · author · university · dateNew search

You searched for subject:(TLC bioautography). Showing records 1 – 3 of 3 total matches.

Search Limiters

Last 2 Years | English Only

No search limiters apply to these results.

▼ Search Limiters


University of the Western Cape

1. Sonka, Luveni. Exploring anti-tyrosinase bioactive compounds from the Cape flora .

Degree: 2018, University of the Western Cape

Tyrosinase is an enzyme widely distributed in the biosphere and is found in many species of bacteria, fungi, animals, and plants; it is associated with melanin production. Even though it possesses many beneficial properties such as photoprotection, but overproduction causes undesirable effects such melasma, solar lentigines etc. Therefore, tyrosinase enzyme inhibitors are of far-ranging importance in cosmetics, medicinal products, and food industries. This study is aimed to test anti-tyrosinase activity in 37 plants from 20 families using mushroom tyrosinase inhibition method; each plant was extracted with methanol. The results showed that 17 plant extracts, exerted a considerable level of in vitro tyrosinase inhibition comparable to positive controls of kojic acid in the same solvent systems when evaluated spectrophotometrically. Among plant extracts, those that showed an inhibition rate >50 % at 50 μg/ml and ˃60 % at 200 μg/ml were A. karroo (Hayne.), A. afra Jacq. Ex Willd, C. geifolia (L.), E. racemosa (L.), H. petiolare Hilliard & B.L.Burt, M. quercifolia (L.), M. communis (L.), P. rigida (Wikstr.), P. ecklonii (Benth.), P. ericoides (L.), S. Africanacaerulea (L.), S. Africana-lutea (L.), S. antarcticus (Willd.), S. lucida (L.) F.A.Barkley, S. hamilifolius (L.), S. furcellata R.Br and T riparia which exhibited great anti-tyrosinase activity. Advisors/Committee Members: Mohammed, Ahmed H.A (advisor).

Subjects/Keywords: CFR, M. quercifolia; TLC bioautography; Tyrosinase Inhibition; Melanoma B16- F10; Melanin biosynthesis inhibition; Depigmentation; cosmetics

Record DetailsSimilar RecordsGoogle PlusoneFacebookTwitterCiteULikeMendeleyreddit

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Sonka, L. (2018). Exploring anti-tyrosinase bioactive compounds from the Cape flora . (Thesis). University of the Western Cape. Retrieved from http://hdl.handle.net/11394/6531

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Sonka, Luveni. “Exploring anti-tyrosinase bioactive compounds from the Cape flora .” 2018. Thesis, University of the Western Cape. Accessed October 21, 2019. http://hdl.handle.net/11394/6531.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Sonka, Luveni. “Exploring anti-tyrosinase bioactive compounds from the Cape flora .” 2018. Web. 21 Oct 2019.

Vancouver:

Sonka L. Exploring anti-tyrosinase bioactive compounds from the Cape flora . [Internet] [Thesis]. University of the Western Cape; 2018. [cited 2019 Oct 21]. Available from: http://hdl.handle.net/11394/6531.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Sonka L. Exploring anti-tyrosinase bioactive compounds from the Cape flora . [Thesis]. University of the Western Cape; 2018. Available from: http://hdl.handle.net/11394/6531

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation


University of the Western Cape

2. Etsassala, Ninon Geornest Eudes Ronauld. Detection of selective tyrosinase inhibitors from some South African plant extracts of lamiaceae family .

Degree: 2016, University of the Western Cape

Various dermatological disorders, such as formation of black pigmented patches on the surface of the skin arise from the over-activity of tyrosinase enzyme's degenerative action. This enzyme is further implicated in the involvement of melanin in malignant melanoma, the most lifethreatening skin tumors. Although, synthetic products were found effective to combat this menace, nevertheless, overtime detrimental effect on human skin is a challenge. Investigation of natural tyrosinase inhibitors from methanol extracts of medicinal plants of Lamiaceae family using L-tyrosine as substrate on three different complementary assays (TLC bio-autography, spectrophotometry and cyclic voltammetry) was carried out accordingly. The result indicated Salvia chamelaeagnea, Salvia dolomitica, Plectranthus ecklonii, Plectranthus namaensis, and Plectranthus zuluensis, with significant zone of inhibition against tyrosinase on TLC bio-autography, spectrophotometry result showed that extracts of Plectranthus ecklonii (IC50 = 21.58 μg/mL), Plectranthus zuluensis (IC50 = 23.99 μg/mL), Plectranthus madagascariensis (IC50 = 23.99 μg/mL) and Salvia lanceolata (IC50 = 28.83) demonstrated good anti-tyrosinase activity when compared with kojic acid (IC50 = 3.607 μg/mL). On the other hand, cyclic voltammetry are in consonant with above results thereby supported the nomination of some of the extracts as strong anti-tyrosinase agents. Salvia chamelaeagnea showed strong activity in cyclic voltammetry and clear zone of inhibition on TLC bioautography, these reasons gave us justification for further chemical study to isolate the bioactive constituents. Phytochemical investigation of the bioactive extract of Salvia chamelaeagnea using different chromatographic methods including column chromatographic and semi preparative HPLC afforded six (6) known compounds viz carsonol (C1), carnosic acid (C2), 7- ethoxylrosmanol (C3), ursolic acid (C4), rosmanol (C5) and ladanein (C6). Their chemical structures were elucidated by analyses of spectroscopic (1H and 13C NMR) data as well as correlations with existing literature. The methanolic extract of S. chamelaeagnea (SC) showed moderate antityrosinase (IC50 = 267.4 μg/mL) activity, total antioxidant capacities measured as: Oxygen radicals absorbance capacity (ORAC; 14970 ± 5.16 μM TE/g), ferric-ion reducing antioxidant power (FRAP; 9869.43 ± 7.87 μM AAE/g) and trolox equivalent absorbance capacity (TEAC; 13706.5 ± 0.95 μM TE/g). Excellent total antioxidant capacities were demonstrated by C1 and C5 respectively as FRAP (9338.92 ± 1.72; 8622.73 ± 1.92) μM AAE/g; TEAC (16505 ± 0.86; 10641.5 ± 0.52) μM TE/g; ORAC (14550.5 ± 3.65; 14633.90 ± 3.84) μM TE/g and including the inhibition of Fe2+ -induced lipid peroxidation (IC50 = 32.5; 30.25) μg/mL. All the compounds except C4 are electro-active with well-defined oxidation-reduction peaks while C1 demonstrated the highest tyrosinase inhibitory activity by strongly decreased the inhibition current with time using cyclic voltammetry method. The isolated compounds especially C1,… Advisors/Committee Members: Mohammed, Ahmed (advisor), Prof. Emmanuel Iwuoha (advisor).

Subjects/Keywords: Lamiaceae; Tyrosinase inhibitor; Cyclic voltammetry; TLC bioautography; Medicinal plants; Cosmetics; Antioxidant; S. chamelaeagnea

Record DetailsSimilar RecordsGoogle PlusoneFacebookTwitterCiteULikeMendeleyreddit

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Etsassala, N. G. E. R. (2016). Detection of selective tyrosinase inhibitors from some South African plant extracts of lamiaceae family . (Thesis). University of the Western Cape. Retrieved from http://hdl.handle.net/11394/5439

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Etsassala, Ninon Geornest Eudes Ronauld. “Detection of selective tyrosinase inhibitors from some South African plant extracts of lamiaceae family .” 2016. Thesis, University of the Western Cape. Accessed October 21, 2019. http://hdl.handle.net/11394/5439.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Etsassala, Ninon Geornest Eudes Ronauld. “Detection of selective tyrosinase inhibitors from some South African plant extracts of lamiaceae family .” 2016. Web. 21 Oct 2019.

Vancouver:

Etsassala NGER. Detection of selective tyrosinase inhibitors from some South African plant extracts of lamiaceae family . [Internet] [Thesis]. University of the Western Cape; 2016. [cited 2019 Oct 21]. Available from: http://hdl.handle.net/11394/5439.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Etsassala NGER. Detection of selective tyrosinase inhibitors from some South African plant extracts of lamiaceae family . [Thesis]. University of the Western Cape; 2016. Available from: http://hdl.handle.net/11394/5439

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation


Univerzitet u Beogradu

3. Nikolić, Miloš M., 1985-. Biološka aktivnost etarskih ulja odabranih aromatičnih biljaka na vrste rodova Staphylococcus, Streptococcus, Lactobacillus, Pseudomonas, Entercoccus i Candida izlovane iz usne duplje čoveka.

Degree: Biološki fakultet, 2016, Univerzitet u Beogradu

Biologija - Mikologija / Biology - Plant Physiology

Oralno zdravlje smatra se važnim delom opšteg zdravlja ljudi, ali je često zanemarena oblast, a razlozi tome su raznovrsni i kompleksni. Visoka učestalost oralnih oboljenja ima veliki socijalni i ekonomski uticaj. Najčešća oboljenja usne duplje su karijes, parodontalna oboljenja i oralne infekcije, dok su glavni uzročnici ovih oboljenja vrste rodova Streptococcus mutans-grupa, Staphylococcus, Lactobacillus, Pseudomonas, Enterococcus i Candida. Efikasan tretman i razvoj novih antiseptika koji poseduju antimikrobnu aktivnost je od velikog značaja za medicinu. Najčešće se koriste preparati koji sadrže hlorheksidin, povidon jod i cetilpiridinijum hlorid, zatim sintetička sredstva kao što su penicilin, cefalosporini, tetraciklini i njihovi derivati, nistatin, amfoterecin B, flukonazol itd. Ovi agensi poseduju antimikrobno dejstvo i kontrolišu upalu desni, ali imaju ograničenu efikasnost i veliki broj negativnih posledica kao što su bojenja zuba, iritacije sluzokože usne duplje, pojave alergije, nefrotoksičnost i endokrinu toksičnost, a povezuju se i sa kancerom i drugim sekundarnim oboljenjima. Problem rezistencije prema Svetskoj zdravstvenoj organizaciji je najveća pretnja globalnom zdravlju čoveka. Jedna od alternativa koja je poslednjih decenija u žiži naučnih interesovanja jesu etarska ulja kao izvor biološki aktivnih jedinjenja. U cilju dobijanja novih biološkli aktivnih jedinjenja, koja neće biti toksična za humane i animalne ćelije, u ovoj disertaciji analiziran je hemijski sastav i biološka aktivnost in vitro 60 komercijalnih uzoraka etarskih ulja ekstrahovano iz 47 biljnih vrsta: Cananga odorata, Foeniculum vulgare, Pimpinella anisum, Pastinaca sativa, Anethum graveolens, Matricaria recutita, Achillea millefolium, Helichrysum italicum, Boswellia carterii, Canarium luzonicum, Commiphora myrrha, Juniperus communis, J. virginiana, Gaultheria procumbens, G. fragrantissima, Pelargonium graveolens, Hyssopus officinalis, Mentha piperita, M. pulegium, Lavandula angustifolia, Thymus algeriensis, T. vulgaris, T. serpyllum, Rosmarinus officinalis, Salvia lavanudilifolia, S. officinalis, S. sclarea, S. triloba, Satureja montana, Laurus nobilis, Leptospermum petersonii, L. scoparium, Eucalyptus citriodora, E. globulus, Melaleuca alternifolia, M. quinquenervia, Syzygium aromaticum, Abies procera, A. grandis, A. alba, Pseudotsuga douglasii, Pinus silvestris, Piper nigrum, Cymbopogon martinii, Rosa centifolia, Citrus aurantium spp. aurantium i C.limon. Cilj rada je ispitivanje biološke aktivnosti i uticaja etarskih ulja i komponenti odabranih aromatičnih biljaka na mikroorganizme izolovane iz usne duplje čoveka i primena u prevenciji i lečenju oralnih bolesti i infekcija...

Advisors/Committee Members: Soković, Marina.

Subjects/Keywords: oral health; Staphylococcus; Streptococcus; Lactobacillus; Pseudomonas; Enterococcus; Candida; essential oils; chemical composition; antibacterial; antifungal; TLC bioautography; scening electronic microscopy; mutual interaction of essential oils and components; quorum sensing; biofilm; cytotoxicity

Record DetailsSimilar RecordsGoogle PlusoneFacebookTwitterCiteULikeMendeleyreddit

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Nikolić, Miloš M., 1. (2016). Biološka aktivnost etarskih ulja odabranih aromatičnih biljaka na vrste rodova Staphylococcus, Streptococcus, Lactobacillus, Pseudomonas, Entercoccus i Candida izlovane iz usne duplje čoveka. (Thesis). Univerzitet u Beogradu. Retrieved from https://fedorabg.bg.ac.rs/fedora/get/o:10383/bdef:Content/get

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Nikolić, Miloš M., 1985-. “Biološka aktivnost etarskih ulja odabranih aromatičnih biljaka na vrste rodova Staphylococcus, Streptococcus, Lactobacillus, Pseudomonas, Entercoccus i Candida izlovane iz usne duplje čoveka.” 2016. Thesis, Univerzitet u Beogradu. Accessed October 21, 2019. https://fedorabg.bg.ac.rs/fedora/get/o:10383/bdef:Content/get.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Nikolić, Miloš M., 1985-. “Biološka aktivnost etarskih ulja odabranih aromatičnih biljaka na vrste rodova Staphylococcus, Streptococcus, Lactobacillus, Pseudomonas, Entercoccus i Candida izlovane iz usne duplje čoveka.” 2016. Web. 21 Oct 2019.

Vancouver:

Nikolić, Miloš M. 1. Biološka aktivnost etarskih ulja odabranih aromatičnih biljaka na vrste rodova Staphylococcus, Streptococcus, Lactobacillus, Pseudomonas, Entercoccus i Candida izlovane iz usne duplje čoveka. [Internet] [Thesis]. Univerzitet u Beogradu; 2016. [cited 2019 Oct 21]. Available from: https://fedorabg.bg.ac.rs/fedora/get/o:10383/bdef:Content/get.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Nikolić, Miloš M. 1. Biološka aktivnost etarskih ulja odabranih aromatičnih biljaka na vrste rodova Staphylococcus, Streptococcus, Lactobacillus, Pseudomonas, Entercoccus i Candida izlovane iz usne duplje čoveka. [Thesis]. Univerzitet u Beogradu; 2016. Available from: https://fedorabg.bg.ac.rs/fedora/get/o:10383/bdef:Content/get

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

.