subject:(T cnicas in vivo)

Addis Ababa University

1. Belay, Tadesse. In Vitro and In Vivo Anti-trypanosomal Activity of Dichloromethane and Methanol Crude Leaf Extracts of Dovyalis abyssinica (Flacourtiaceae) against Trypanosoma congolense Field Isolate Belay Tadesse A Thesis Submitted to the Department of Pharmacology and Clinical Pharmacy Presented in Partial Fulfillment of the Requirements for the Degree of Master of Science (Pharmacology) Addis Ababa University Addis Ababa, Ethiopia .

Degree: 2014, Addis Ababa University

URL: http://etd.aau.edu.et/dspace/handle/123456789/6072

► In vitro and in vivo anti-trypanosomal activity of dichloromethane and methanol crude leaf extracts of Dovyalis abyssinica (Flacourtiaceae) against Trypanosoma congolense field isolate Belay Tadesse… (more)

▼ In vitro and in vivo anti-trypanosomal activity of dichloromethane and methanol crude leaf extracts of Dovyalis abyssinica (Flacourtiaceae) against Trypanosoma congolense field isolate Belay Tadesse Addis Ababa University, 2014 African Trypanosomosis is a neglected tropical disease of medical and veterinary importance. Parasite control relies on the use of few drugs whose resistance and unacceptable toxicities urged the investigation of new agents, preferably from natural sources. Though Dovyalis abyssinica has been reported to posses significant trypanocidal activity on Trypanosoma brucei in in vitro model, activity on trypanosome infected laboratory animals has not yet been worked out. The aim of the present study was, therefore, to investigate the in vitro and in vivo activity of D. abyssinica on T. congolense field isolate. To evaluate the effect on motility, 200 μl T. congolense infected blood was mixed with 50 μl of 20, 10, 2, 0.1, 0.015 mg/ml dichloromethane and methanol extracts. Reduction or cessation of motility was then microscopically monitored for 120 minutes, and the remaining in vitro mixtures were inoculated to healthy mice and monitored for development of infection for 21 days. Furthermore, fifty T. congolense infected mice were randomly grouped into ten groups of five and administered with curative doses (250, 200, 150 and 100 mg/kg) of dichloromethane and methanol and 28 mg/kg diminazene aceturate and dimethylsulfoxide. Following administration, parasitemia, packed cell volume (PCV), rectal temperature, body weight and survival time were iii monitored. Suppressive doses of the extracts (250 and 200 mg/kg) were administered 24 hours post-infection and parasitemia was monitored. Dichloromethane and methanol extracts at 20, 10, 2 mg/ml concentrations ceased parasite motility within one hour and eliminated subsequent infectivity in mice for 21 days. Administration of dichloromethane and methanol extracts at 250 and 200 mg/kg reduced (p<0.05) parasitemia and rectal temperature, and improved (p<0.05) PCV, mean body weight, and mean survival time compared to DMSO treatment. In conclusion, D. abyssinica at higher concentrations in vitro and higher curative doses in vivo in mice posses anti-trypanosomal activity. Keywords: Anti-trypanosomal, D. abyssinica, in vitro, in vivo, T. congolense, mice. Advisors/Committee Members: Getachew Abebe(Prof) (advisor).

Subjects/Keywords: Anti-trypanosomal; D. abyssinica; in vitro; in vivo; T. congolense; mice

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Belay, T. (2014). In Vitro and In Vivo Anti-trypanosomal Activity of Dichloromethane and Methanol Crude Leaf Extracts of Dovyalis abyssinica (Flacourtiaceae) against Trypanosoma congolense Field Isolate Belay Tadesse A Thesis Submitted to the Department of Pharmacology and Clinical Pharmacy Presented in Partial Fulfillment of the Requirements for the Degree of Master of Science (Pharmacology) Addis Ababa University Addis Ababa, Ethiopia . (Thesis). Addis Ababa University. Retrieved from http://etd.aau.edu.et/dspace/handle/123456789/6072

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Belay, Tadesse. “In Vitro and In Vivo Anti-trypanosomal Activity of Dichloromethane and Methanol Crude Leaf Extracts of Dovyalis abyssinica (Flacourtiaceae) against Trypanosoma congolense Field Isolate Belay Tadesse A Thesis Submitted to the Department of Pharmacology and Clinical Pharmacy Presented in Partial Fulfillment of the Requirements for the Degree of Master of Science (Pharmacology) Addis Ababa University Addis Ababa, Ethiopia .” 2014. Thesis, Addis Ababa University. Accessed March 05, 2021. http://etd.aau.edu.et/dspace/handle/123456789/6072.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Belay, Tadesse. “In Vitro and In Vivo Anti-trypanosomal Activity of Dichloromethane and Methanol Crude Leaf Extracts of Dovyalis abyssinica (Flacourtiaceae) against Trypanosoma congolense Field Isolate Belay Tadesse A Thesis Submitted to the Department of Pharmacology and Clinical Pharmacy Presented in Partial Fulfillment of the Requirements for the Degree of Master of Science (Pharmacology) Addis Ababa University Addis Ababa, Ethiopia .” 2014. Web. 05 Mar 2021.

Vancouver:

Belay T. In Vitro and In Vivo Anti-trypanosomal Activity of Dichloromethane and Methanol Crude Leaf Extracts of Dovyalis abyssinica (Flacourtiaceae) against Trypanosoma congolense Field Isolate Belay Tadesse A Thesis Submitted to the Department of Pharmacology and Clinical Pharmacy Presented in Partial Fulfillment of the Requirements for the Degree of Master of Science (Pharmacology) Addis Ababa University Addis Ababa, Ethiopia . [Internet] [Thesis]. Addis Ababa University; 2014. [cited 2021 Mar 05]. Available from: http://etd.aau.edu.et/dspace/handle/123456789/6072.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Belay T. In Vitro and In Vivo Anti-trypanosomal Activity of Dichloromethane and Methanol Crude Leaf Extracts of Dovyalis abyssinica (Flacourtiaceae) against Trypanosoma congolense Field Isolate Belay Tadesse A Thesis Submitted to the Department of Pharmacology and Clinical Pharmacy Presented in Partial Fulfillment of the Requirements for the Degree of Master of Science (Pharmacology) Addis Ababa University Addis Ababa, Ethiopia . [Thesis]. Addis Ababa University; 2014. Available from: http://etd.aau.edu.et/dspace/handle/123456789/6072

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

2. Joly, Candie. Dynamique des réponses lymphocytaires T locales et systémiques à l'injection d'un vaccin dans la peau : Dynamic of local and systemic cellular responses after vaccination in the skin.

Degree: Docteur es, Immunologie, 2019, Université Paris-Saclay (ComUE)

URL: http://www.theses.fr/2019SACLS317

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La vaccination est considérée comme l’un des plus grandes découvertes de l’histoire des maladies infectieuses, ayant permis le déclin et l’éradication de plusieurs pathogènes. Cependant,… (more)

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La vaccination est considérée comme l’un des plus grandes découvertes de l’histoire des maladies infectieuses, ayant permis le déclin et l’éradication de plusieurs pathogènes. Cependant, nous ignorons encore tous les mécanismes impliqués dans la protection contre les pathogènes. Cette méconnaissance est la cause de notre incapacité à formuler des nouveaux vaccins contre le VIH, la tuberculose, le paludisme et les pathogènes émergents. Récemment, on note des efforts pour induire une réponse cellulaire efficace après une vaccination, qui joue un rôle crucial dans la clairance des pathogènes.Cette thèse s’appuie sur un modèle de vaccin vivant atténue issu du virus de la vaccine : le MVA (Modified Vaccinia Ankara) et sur le modèle de primate non-humain. Nous avons caractérisé la réponse cellulaire après une immunisation intradermique suivant un schéma en prime-boost homologue, avec un boost à 2, suivi d’un boost à 9 mois. Le MVA a induit une infiltration massive de Lymphocytes T CD8 au niveau du site d’injection, 7 jours après l’immunisation. La réponse cellulaire systémique était modérée et ne reflétait pas l’amplitude de la réponse locale. Les injections du prime et du boost ont orienté la réponse cellulaire de façon différente, ce qui a mené à une importante induction de cellules T CD4 et CD8, persistantes, spécifiques de l’antigène et polyfonctionnelles après l’injection du boost à 9 mois.Cette étude souligne la différence entre les réponses systémiques et locales, démontrant l’importance de se focaliser sur la réponse tissulaire. Elle a également mis en lumière l’impact du schéma d’immunisation sur la qualité de la réponse cellulaire.

Vaccination has been considered as one of the greatest discoveries in the history of infectious diseases by allowing pathogens decline or eradication. However, we still ignore all the mechanism that lead to protection and therefore, fail to elaborate new vaccines against HIV, tuberculosis, malaria and emergent pathogens. Recently, efforts have been made to elicit effective cellular response after vaccination, which is crucial for pathogen clearance.This thesis relied on live-attenuated vaccine model derived from the vaccinia virus: the MVA (Modified Vaccinia Ankara) and a non-human primate model. We characterized the cellular immune response triggered by a homologous prime-boost intradermal injection of MVA, with a 2 months and 9 months boost. The MVA induced a massive infiltration of CD8 T cells at the injection site 7 days post immunization. In comparison, the systemic cellular response was mild and did not reflect the magnitude of the local response. The prime and boost injections elicited distinct orientation of the systemic and local T cells, which led to an important induction of a persistent, antigen-specific and polyfunctional CD8 and CD4 T cell responses after the 9 months boost.This work emphasizes the difference between local and systemic response, demonstrating the importance of the focus on tissue immunity. It also highlights the impact of the immunization schedule on…

Advisors/Committee Members: Le Grand, Roger (thesis director), Martinon, Frédéric (thesis director).

Subjects/Keywords: Réponse cellulaire T; Modèle primate non humain; Mva; Imagerie in vivo; T cellular response; Non human primate model; Mva; In vivo imaging

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Joly, C. (2019). Dynamique des réponses lymphocytaires T locales et systémiques à l'injection d'un vaccin dans la peau : Dynamic of local and systemic cellular responses after vaccination in the skin. (Doctoral Dissertation). Université Paris-Saclay (ComUE). Retrieved from http://www.theses.fr/2019SACLS317

Chicago Manual of Style (16^th Edition):

Joly, Candie. “Dynamique des réponses lymphocytaires T locales et systémiques à l'injection d'un vaccin dans la peau : Dynamic of local and systemic cellular responses after vaccination in the skin.” 2019. Doctoral Dissertation, Université Paris-Saclay (ComUE). Accessed March 05, 2021. http://www.theses.fr/2019SACLS317.

MLA Handbook (7^th Edition):

Joly, Candie. “Dynamique des réponses lymphocytaires T locales et systémiques à l'injection d'un vaccin dans la peau : Dynamic of local and systemic cellular responses after vaccination in the skin.” 2019. Web. 05 Mar 2021.

Vancouver:

Joly C. Dynamique des réponses lymphocytaires T locales et systémiques à l'injection d'un vaccin dans la peau : Dynamic of local and systemic cellular responses after vaccination in the skin. [Internet] [Doctoral dissertation]. Université Paris-Saclay (ComUE); 2019. [cited 2021 Mar 05]. Available from: http://www.theses.fr/2019SACLS317.

Council of Science Editors:

Joly C. Dynamique des réponses lymphocytaires T locales et systémiques à l'injection d'un vaccin dans la peau : Dynamic of local and systemic cellular responses after vaccination in the skin. [Doctoral Dissertation]. Université Paris-Saclay (ComUE); 2019. Available from: http://www.theses.fr/2019SACLS317

3. Costa, Paula Tatiana de Oliveira da. Efeito da interven??o cl?nica para manejo de estresse a partir de t?cnicas de biofeedback.

Degree: 2011, Universidade Federal do Amazonas

URL: http://tede.ufam.edu.br/handle/tede/3927

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O estresse em suas diversas formas de express?o vem ocupando os ranques de maior incid?ncia de danos ? sa?de, causando efeitos na sa?de ps?quica e… (more)

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O estresse em suas diversas formas de express?o vem ocupando os ranques de maior incid?ncia de danos ? sa?de, causando efeitos na sa?de ps?quica e fisiol?gica. Sabe-se ainda, que a principal fonte de estresse se encontra no interior de cada indiv?duo, a partir da forma como cada um interpreta cada um dos eventos de sua vida, somada a uma predisposi??o gen?tica associada aos est?mulos da intera??o social, familiar, rela??o com parentes, amigos, experi?ncias de vida, etc. Pode-se dizer que o estresse ? uma resposta complexa do organismo, envolvendo rea??es hormonais, f?sicas e psicol?gicas frente a qualquer evento que seja interpretado como desafiante, o qual instiga a necessidade de adapta??o para preservar o bem-estar e a vida. A presente pesquisa objetivou o estudo dos efeitos da interven??o cl?nica para manejo de estresse a partir de t?cnicas cognitivo-comportamentais e de biofeedback. Dentre as propostas terapeuticas da abordagem cognitiva-comportamental para o enfrentamento desta condi??o, foi utilizada o treino comportamental sob forma de t?cnicas de relaxamento e o biofeedback, como t?cnica auxiliar, que foi aplicada por meio do monitoramento da Resposta Galv?nica da Pele (RGP), atrav?s de um instrumento que retroalimenta informa??es fisiol?gicas para um monitor as tornando pass?veis de controle volunt?rio. Partiu-se da aplica??o de um roteiro padronizado com seis sess?es e sua reavalia??o ap?s um intervalo de tempo de 25 meses (em m?dia). Participaram voluntariamente do presente estudo 23 indiv?duos, sendo 73,9% do sexo feminino e 26,1% do sexo masculino, com idade m?dia de 40,80 e desvio padr?o de 11,15. 65,2% da amostra s?o do Estado do Amazonas e o restante corresponde ?s demais Unidades Federativas do Brasil. A avalia??o dos resultados propiciou conhecimento sobre uso estrat?gias adquiridas como a pr?tica da respira??o diafragm?tica, assim como os benef?cios advindos do treinamento como melhora no relacionamento familiar, ambiente de trabalho, comportamento no tr?nsito e autorregula??o emocional. Este estudo revelou-se de importante relev?ncia social por promover melhora significativa e duradoura em seus participantes. Contudo, se faz necess?rio a continuidade dos estudos na ?rea, al?m do aprimoramento do roteiro j? constru?do.

Stress in its various forms of expression has been occupying the ranks of higher incidence of injury to health, causing effects on physiological and psychological health. It is known that the main source lies within each individual, from the way each interprets each of the events of his life, coupled with a genetic predisposition to the stimulus of social interaction, family, and relationship with relatives, friends, life experiences, etc. You could say that stress is a complex response of the organism, involving hormonal reactions, physical and psychological against any event that is interpreted as challenging, which instigates the need for adaptation to preserve the well-being and life. This research aimed to study the effects of clinical intervention for stress management…

Advisors/Committee Members: Silva Filho, Jos? Humberto da, 352.152.404-34, http://lattes.cnpq.br/6407212894793482.

Subjects/Keywords: Manejo de estresse; Biofeedback; T?cnicas de relaxamento; Stress management; Relaxation techniques; CI?NCIAS HUMANAS: PSICOLOGIA

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Costa, P. T. d. O. d. (2011). Efeito da interven??o cl?nica para manejo de estresse a partir de t?cnicas de biofeedback. (Masters Thesis). Universidade Federal do Amazonas. Retrieved from http://tede.ufam.edu.br/handle/tede/3927

Chicago Manual of Style (16^th Edition):

Costa, Paula Tatiana de Oliveira da. “Efeito da interven??o cl?nica para manejo de estresse a partir de t?cnicas de biofeedback.” 2011. Masters Thesis, Universidade Federal do Amazonas. Accessed March 05, 2021. http://tede.ufam.edu.br/handle/tede/3927.

MLA Handbook (7^th Edition):

Costa, Paula Tatiana de Oliveira da. “Efeito da interven??o cl?nica para manejo de estresse a partir de t?cnicas de biofeedback.” 2011. Web. 05 Mar 2021.

Vancouver:

Costa PTdOd. Efeito da interven??o cl?nica para manejo de estresse a partir de t?cnicas de biofeedback. [Internet] [Masters thesis]. Universidade Federal do Amazonas; 2011. [cited 2021 Mar 05]. Available from: http://tede.ufam.edu.br/handle/tede/3927.

Council of Science Editors:

Costa PTdOd. Efeito da interven??o cl?nica para manejo de estresse a partir de t?cnicas de biofeedback. [Masters Thesis]. Universidade Federal do Amazonas; 2011. Available from: http://tede.ufam.edu.br/handle/tede/3927

4. Nader, Joëlle. Modèles précliniques d’étude du mésothéliome : application à l’évaluation de la virothérapie anti-tumorale in vivo : Experimental models of malignant mesothelioma for the evaluation of in vivo anti-tumoral virotherapy.

Degree: Docteur es, Biologie, médecine et santé, 2017, Nantes

URL: http://www.theses.fr/2017NANT1043

►

Au cours de ma thèse, j’ai travaillé sur deux sujets complémentaires. Le premier a concerné l’étude des interactions entre les cellules tumorales et leur microenvironnement,… (more)

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Au cours de ma thèse, j’ai travaillé sur deux sujets complémentaires. Le premier a concerné l’étude des interactions entre les cellules tumorales et leur microenvironnement, dans quatre modèles de mésothéliomes malins (MM) de rat. Les analyses histologiques du stroma, associées à des analyses protéomiques et de l’expression de différentes cytokines/chimiokines ont permis d’identifier trois stades d’invasivité croissante, associés à des modifications quantitatives de nombreuses protéines et à un infiltrat immunitaire décroissant. La tumeur la plus invasive a été caractérisée par une immunosuppression avec un profil moléculaire spécifique augmentant le potentiel métastatique. Le second sujet a concerné l’évaluation de l’efficacité de la virothérapie anti-tumorale, basée sur l’utilisation de la souche vaccinale du virus de la rougeole MV et de son variant MV-ΔC, pour le traitement du MM. Les deux virus ont induit des régressions tumorales chez des souris NOD SCID transplantées avec deux lignées de MM humains, le MV-ΔC amplifiant cet effet en induisant une mort cellulaire plus rapide, attestée par une réduction plus marquée de la masse tumorale. Ce potentiel apoptotique est associé, in vitro, à une production accrue du signal de danger HMGB1 et à la synthèse d’une grande quantité d’ARN double brin viral. Ces cellules infectées par le MV-ΔC sont aussi capables de favoriser la maturation des cellules dendritiques grâce à la réplication virale et à l’activation de Protéine Kinase R. Ce travail de caractérisation de nouveaux modèles immunocompétents et de nouvelles stratégies thérapeutiques permet d'envisager un meilleur traitement des patients souffrant de mésothéliome.

As a PhD student, I worked in parallel on two complementary subjects. The first one concerned the study of the interactions between tumor cells and their microenvironment in four models of rat malignant mesothelioma (MM), differing in both their immune infiltrate and their metastatic potential. Histological analyses of stroma, together with proteomic analyses and expression of different cytokines, chemokines and growth factors, led us to the identification of three stages of increasing invasiveness, associated with quantitative changes in many proteins and a decreased immune infiltrate. The most invasive tumor was characterized by immunosuppression with a specific molecular profile increasing the metastatic potential. The second topic was the evaluation of the efficacy of anti-tumor virotherapy, based on the use of the Schwarz strain of measles virus (MV) and its variant MV-ΔC for the treatment of MM. Both viruses induced tumor regressions in NOD SCID mice transplanted with two human MM cell lines, but MV-ΔC amplified this effect by inducing faster cell death, as revealed by a marked reduction of the tumor mass. This apoptotic potential is associated, in vitro, with an increased production of the danger signal HMGB1 and the synthesis of a large amount of viral double-stranded RNA. These MV-ΔC-infected cells are also capable of promoting the maturation of…

Advisors/Committee Members: Pouliquen, Daniel (thesis director), Boisgerault, Nicolas (thesis director).

Subjects/Keywords: Modèles in vivo

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Nader, J. (2017). Modèles précliniques d’étude du mésothéliome : application à l’évaluation de la virothérapie anti-tumorale in vivo : Experimental models of malignant mesothelioma for the evaluation of in vivo anti-tumoral virotherapy. (Doctoral Dissertation). Nantes. Retrieved from http://www.theses.fr/2017NANT1043

Chicago Manual of Style (16^th Edition):

Nader, Joëlle. “Modèles précliniques d’étude du mésothéliome : application à l’évaluation de la virothérapie anti-tumorale in vivo : Experimental models of malignant mesothelioma for the evaluation of in vivo anti-tumoral virotherapy.” 2017. Doctoral Dissertation, Nantes. Accessed March 05, 2021. http://www.theses.fr/2017NANT1043.

MLA Handbook (7^th Edition):

Nader, Joëlle. “Modèles précliniques d’étude du mésothéliome : application à l’évaluation de la virothérapie anti-tumorale in vivo : Experimental models of malignant mesothelioma for the evaluation of in vivo anti-tumoral virotherapy.” 2017. Web. 05 Mar 2021.

Vancouver:

Nader J. Modèles précliniques d’étude du mésothéliome : application à l’évaluation de la virothérapie anti-tumorale in vivo : Experimental models of malignant mesothelioma for the evaluation of in vivo anti-tumoral virotherapy. [Internet] [Doctoral dissertation]. Nantes; 2017. [cited 2021 Mar 05]. Available from: http://www.theses.fr/2017NANT1043.

Council of Science Editors:

Nader J. Modèles précliniques d’étude du mésothéliome : application à l’évaluation de la virothérapie anti-tumorale in vivo : Experimental models of malignant mesothelioma for the evaluation of in vivo anti-tumoral virotherapy. [Doctoral Dissertation]. Nantes; 2017. Available from: http://www.theses.fr/2017NANT1043

UCLA

5. Gschweng, Eric Hans. Cancer Immunotherapy Using Engineered Hematopoietic Stem Cells.

Degree: Microbiology, Immunology, & Molecular Genetics, 2015, UCLA

URL: http://www.escholarship.org/uc/item/4684d7vk

► Engineering the immune system against cancer ideally provides surgical precision against the antigen bearing target cell while avoiding the systemic, off-target toxicity of chemotherapy. Successful… (more)

▼ Engineering the immune system against cancer ideally provides surgical precision against the antigen bearing target cell while avoiding the systemic, off-target toxicity of chemotherapy. Successful treatment of patients in the clinic has been achieved by the expression of anti-cancer T-cell receptors (TCR) and chimeric antigen receptors (CAR) in T cells followed by infusion of these cells into cancer patients. Unfortunately, while many patients initially respond showing anti-tumor efficacy, the effects are by and large transient with the majority of patients succumbing to disease. This observation speaks to the power of the engineered antigen receptor, but also indicates a need for a persistent source of cells to mount a continuous response.This thesis work sought to investigate the feasibility of engineering immunity using hematopoietic stem cells (HSC). HSC are at the top of the hematopoietic hierarchy, and would theoretically provide a continuous supply of antigen receptor bearing T cells capable of eliminating disease in a patient. Important considerations for using HSCs in gene therapy include: efficient gene delivery to HSCs which are refractory to gene modification by nature, robust expression of the transgene within gene modified cells to provide efficacy, evaluation of successful gene modification and engraftment following transplant, the ability of gene modified T cells to mount an immune response against their intended targets, and the ability to eliminate the gene modified cells in the event of undesired outcomes.Current systems to study human HSC biology and gene therapy are limited, and the best available are human / mouse chimeric transplant systems. Using these models, we evaluated the utility of positron emission tomography (PET) in the humanized mouse following transplant of gene modified cells, and found it superior to peripheral blood flow cytometry in being able to temporally establish successful engraftment. Using a lentiviral vector expressing both a TCR against a common cancer / testes antigen, NY-ESO-1, as well as a PET imaging / suicide gene, we demonstrated the ability to monitor engraftment, successful generation of effector cells capable of killing patient derived cancer cell lines, and the complete elimination of gene modified cells. This final work provides support for a clinical trial soon to enroll patients at UCLA.The use of HSCs for engineered cancer immunotherapy could overcome current limitations associated with the lack of persistence of engineered terminally differentiated immune cells. Further, the rich clinical history of gene therapy using HSCs provides a broad platform for future studies aimed at the broad spectrum of cancer disease.

Subjects/Keywords: Immunology; Pharmacology; Engineered immunotherapy; Gene therapy; Hematopoietic stem cell; In vivo imaging; Suicide gene; T cell development

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Gschweng, E. H. (2015). Cancer Immunotherapy Using Engineered Hematopoietic Stem Cells. (Thesis). UCLA. Retrieved from http://www.escholarship.org/uc/item/4684d7vk

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Gschweng, Eric Hans. “Cancer Immunotherapy Using Engineered Hematopoietic Stem Cells.” 2015. Thesis, UCLA. Accessed March 05, 2021. http://www.escholarship.org/uc/item/4684d7vk.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Gschweng, Eric Hans. “Cancer Immunotherapy Using Engineered Hematopoietic Stem Cells.” 2015. Web. 05 Mar 2021.

Vancouver:

Gschweng EH. Cancer Immunotherapy Using Engineered Hematopoietic Stem Cells. [Internet] [Thesis]. UCLA; 2015. [cited 2021 Mar 05]. Available from: http://www.escholarship.org/uc/item/4684d7vk.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Gschweng EH. Cancer Immunotherapy Using Engineered Hematopoietic Stem Cells. [Thesis]. UCLA; 2015. Available from: http://www.escholarship.org/uc/item/4684d7vk

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan

6. McKinstry, Karl Kai. Quantitative analysis of antigen-mediated CD4 T cell - CD4 T cell cooperation determining the Th1/Th2 phenotype of a primary immune response.

Degree: 2005, University of Saskatchewan

URL: http://hdl.handle.net/10388/etd-05052005-153624

► Several variables have been found to affect the Th1/Th2 differentiation of newly activated CD4 T cells. This phenotype can be critical in determining effectiveness of… (more)

▼ Several variables have been found to affect the Th1/Th2 differentiation of newly activated CD4 T cells. This phenotype can be critical in determining effectiveness of immune responses. Experiments in this thesis were undertaken to better define the in-vivo cellular interactions involved in determining the Th1/Th2 phenotype of newly activated CD4 T cells.Lethally irradiated BALB/c mice reconstituted with a constant number of syngeneic, naive spleen cells were challenged with xenogeneic red blood cells (XRBC) conjugated to ovalbumin (OVA) and the Th1/Th2 phenotype of the anti-XRBC response assessed. Antigen-specific interferon-gamma (IFN-g) and interleukin-4 (IL-4) secreting cells obtained from spleens of immunized mice were enumerated by an ELISPOT assay; the relative number of IFN-g- and IL-4-producing cells is taken as a relative measure of Th1 and Th2 components of the response. When challenged with a ‘standard’ dose of XRBC-OVA, predominant Th1 responses are generated; when challenged with a ten-fold lower dose, such reconstituted mice do not generate significant responses. This adoptive transfer system was employed to explore further the relationships between quantitative changes in the dose of immunizing antigen and the number of responding antigen-specific CD4 T cells, and the Th1/Th2 phenotype of immune responses generated. Unprimed transgenic CD4 T cells specific for OVA can modulate the Th1/Th2 phenotype of the anti-XRBC response upon immunization with XRBC-OVA. Addition of a small number of naive transgenic spleen cells to the standard reconstituting population of normal spleen cells results in the generation of significant numbers of SRBC-specific Th2 cells when mice are challenged with a ‘standard’ dose, or can generate predominant Th1 responses when mice are challenged with a ten-fold lower dose. Transgenic cells only impact the Th1/Th2 phenotype of CD4 T cells specific for XRBC when OVA is linked to the XRBC. That CD4 T cells specific for different antigens cooperate only through the recognition of linked antigenic determinants has important implications for many aspects of immune regulation. Observations further show that thymocytes from transgenic mice can influence the XRBC-specific response phenotype in an identical manner as transgenic spleen cells, suggesting that previously polarized pro-Th1/Th2 cells are not required in the cooperative events influencing Th1/Th2 phenotype of newly activated CD4 T cells.These observations lead to a quantitative description, whereby antigen-mediated CD4 T cell cooperation can affect the Th1/Th2 phenotype of a primary antigen-specific immune response, and provide a context for further analysis at the molecular level. Advisors/Committee Members: Bretscher, Peter A., Ogunremi, Oladele A., Howard, S. Peter, Havele, Calliopi, Gordon, John R., Delespesse, Guy.

Subjects/Keywords: immune regulation; linked recognition; CD4 T cells; in vivo

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

McKinstry, K. K. (2005). Quantitative analysis of antigen-mediated CD4 T cell - CD4 T cell cooperation determining the Th1/Th2 phenotype of a primary immune response. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/etd-05052005-153624

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

McKinstry, Karl Kai. “Quantitative analysis of antigen-mediated CD4 T cell - CD4 T cell cooperation determining the Th1/Th2 phenotype of a primary immune response.” 2005. Thesis, University of Saskatchewan. Accessed March 05, 2021. http://hdl.handle.net/10388/etd-05052005-153624.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

McKinstry, Karl Kai. “Quantitative analysis of antigen-mediated CD4 T cell - CD4 T cell cooperation determining the Th1/Th2 phenotype of a primary immune response.” 2005. Web. 05 Mar 2021.

Vancouver:

McKinstry KK. Quantitative analysis of antigen-mediated CD4 T cell - CD4 T cell cooperation determining the Th1/Th2 phenotype of a primary immune response. [Internet] [Thesis]. University of Saskatchewan; 2005. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/10388/etd-05052005-153624.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

McKinstry KK. Quantitative analysis of antigen-mediated CD4 T cell - CD4 T cell cooperation determining the Th1/Th2 phenotype of a primary immune response. [Thesis]. University of Saskatchewan; 2005. Available from: http://hdl.handle.net/10388/etd-05052005-153624

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Brunel University

7. Omodho, Becky. Early growth response gene (Egr) 2 and 3 control inflammatory responses of tolerant T cells.

Degree: PhD, 2016, Brunel University

URL: http://bura.brunel.ac.uk/handle/2438/13516 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.699283

► This study investigated the role of tolerance induction in an inflammatory setting in regard to the early growth response genes Egr2 and Egr3. T cells… (more)

Subjects/Keywords: 616.07; Anergy induction in-vitro; Tolerance induction in-vivo; Egr2/3 in tolerance induction; T cell proliferative responses; Transcription factor in tolerance

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APA (6^th Edition):

Omodho, B. (2016). Early growth response gene (Egr) 2 and 3 control inflammatory responses of tolerant T cells. (Doctoral Dissertation). Brunel University. Retrieved from http://bura.brunel.ac.uk/handle/2438/13516 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.699283

Chicago Manual of Style (16^th Edition):

Omodho, Becky. “Early growth response gene (Egr) 2 and 3 control inflammatory responses of tolerant T cells.” 2016. Doctoral Dissertation, Brunel University. Accessed March 05, 2021. http://bura.brunel.ac.uk/handle/2438/13516 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.699283.

MLA Handbook (7^th Edition):

Omodho, Becky. “Early growth response gene (Egr) 2 and 3 control inflammatory responses of tolerant T cells.” 2016. Web. 05 Mar 2021.

Vancouver:

Omodho B. Early growth response gene (Egr) 2 and 3 control inflammatory responses of tolerant T cells. [Internet] [Doctoral dissertation]. Brunel University; 2016. [cited 2021 Mar 05]. Available from: http://bura.brunel.ac.uk/handle/2438/13516 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.699283.

Council of Science Editors:

Omodho B. Early growth response gene (Egr) 2 and 3 control inflammatory responses of tolerant T cells. [Doctoral Dissertation]. Brunel University; 2016. Available from: http://bura.brunel.ac.uk/handle/2438/13516 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.699283

8. Mezajoug Kenfack, Laurette Blandine. Propriétés nutritionnelles et fonctionnelles des protéines de tourteaux, de concentrats et d'isolats de Ricinodendron heudelotii (Bail.) Pierre ex Pax et de Tetracarpidium conophorum (Müll. Arg.) : Nutritional and functional properties of proteins from defatted flours, concentrates and isolates of Ricinodendron heudelotii (Bail.) Pierre ex Pax and Tetracarpidium conophorum (Müll. Arg).

Degree: Docteur es, Procédés biotechnologiques et alimentaires, 2010, Vandoeuvre-les-Nancy, INPL; Université de Ngaoundéré – ENSAI - Cameroun

URL: http://www.theses.fr/2010INPL013N

►

Cette étude a été menée dans le but d’explorer les nouvelles sources de protéines à valeur nutraceutique. Les graines de Ricinodendron heudelotii (Bail. Pierre ex… (more)

▼

Cette étude a été menée dans le but d’explorer les nouvelles sources de protéines à valeur nutraceutique. Les graines de Ricinodendron heudelotii (Bail. Pierre ex Pax) et de Tetracarpidium conophorum (Müll. Arg) ont d’abord été cuites dans de l’eau bouillante pendant 90 et 30 min qui sont respectivement leurs temps optimums de cuisson. Après délipidation et tamisage des tourteaux, la fraction 400 - 500 µm s’est révélée la plus représentative avec plus de 70 % et riche en azote protéique (6–7% MS). Les concentrats et les isolats protéiques ont été préparés à partir des tourteaux respectivement dans l’eau distillée à pH 4,5 et dans une solution de NaOH à 0,2 % (R. heudelotii), une solution de NaCl 0,6 M (T. conophorum) à pH 11. Ces concentrats (65 – 75 % MS de protéines) et ces isolats protéiques (81 – 92 % MS de protéines) ont une composition physico-chimique différente (P < 0,05) de celle des tourteaux. Pour les deux Euphorbiacées, les capacités de rétention d’eau (367 – 467 g / 100 g d’échantillon), de rétention d’huile (256 – 410 g / 100 g d’échantillon) et moussante (68 – 71 %) sont maximales dans les isolats protéiques tandis que les capacités gélifiante (6 – 14 %) et émulsifiante (63 – 87 %) le sont dans les concentrats protéiques. Les teneurs en acides aminés essentiels des tourteaux de R. heudelotii et de T. conophorum sont comparables à celle de la protéine de référence. L’étude de la digestibilité enzymatique in vitro a montré que l’azote libéré après 6 h est supérieur à 90 % dans les concentrats et les isolats protéiques. La digestibilité protéique in vivo indique que le gain de poids des rats mâles âgés de 21 ± 3 jours durant 15 jours d’expérimentation ainsi que les paramètres de rétention azotée sont plus importants avec les régimes à base de l’aliment de référence (caséine) et du tourteau de T. conophorum. Les valeurs corrigées des paramètres de digestibilité par l’indice chimique des acides aminés laissent apparaître que le tourteau de T. conophorum renferme les protéines de très bonne qualité nutritionnelle, autant que la caséine

This study was conducted in order to look for alternative sources of proteins having nutraceutic value. The grains of Ricinodendron heudelotii (Bail.) and Tetracarpidium conophorum (Müll. Arg) were first cooked in boiled water at their optimal cooking time for 90 and 30 min respectively. After defating, sieving of the defatted cakes showed that samples with a granulometry of 400 - 500 µm were most representative (more than 70%), containing more proteic nitrogen (6–7 %). Protein concentrates and protein isolates were prepared from defatted cakes respectively in distilled water at pH 4.5 and in NaOH 0.2% (R. heudelotii) and NaCl 0.6M (T. conophorum) at pH 11. Physico-chemical properties of protein concentrates (65 – 75 % of proteins) and protein isolates (81–92 % of proteins) were different from those of the defatted cakes. Water holding (367 – 467 g / 100 g of sample), oil holding (256 – 410 g / 100 g of sample) and foaming capacities (68 – 71 %) were highest with protein…

Advisors/Committee Members: Linder, Michel (thesis director), Tchiegang, Clergé (thesis director).

Subjects/Keywords: R. heudelotii; Digestibilité in vivo; Compositions en acides aminés; Isolats protéiques; Concentrats protéiques; Tourteaux; T. conophorum; R. heudelotii; Iin vitro digestibility; In vivo digestibility; Amino acids compositions; Defatted cake; Protein concentrates; T. conophorum; Protein isolates

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APA (6^th Edition):

Mezajoug Kenfack, L. B. (2010). Propriétés nutritionnelles et fonctionnelles des protéines de tourteaux, de concentrats et d'isolats de Ricinodendron heudelotii (Bail.) Pierre ex Pax et de Tetracarpidium conophorum (Müll. Arg.) : Nutritional and functional properties of proteins from defatted flours, concentrates and isolates of Ricinodendron heudelotii (Bail.) Pierre ex Pax and Tetracarpidium conophorum (Müll. Arg). (Doctoral Dissertation). Vandoeuvre-les-Nancy, INPL; Université de Ngaoundéré – ENSAI - Cameroun. Retrieved from http://www.theses.fr/2010INPL013N

Chicago Manual of Style (16^th Edition):

Mezajoug Kenfack, Laurette Blandine. “Propriétés nutritionnelles et fonctionnelles des protéines de tourteaux, de concentrats et d'isolats de Ricinodendron heudelotii (Bail.) Pierre ex Pax et de Tetracarpidium conophorum (Müll. Arg.) : Nutritional and functional properties of proteins from defatted flours, concentrates and isolates of Ricinodendron heudelotii (Bail.) Pierre ex Pax and Tetracarpidium conophorum (Müll. Arg).” 2010. Doctoral Dissertation, Vandoeuvre-les-Nancy, INPL; Université de Ngaoundéré – ENSAI - Cameroun. Accessed March 05, 2021. http://www.theses.fr/2010INPL013N.

MLA Handbook (7^th Edition):

Mezajoug Kenfack, Laurette Blandine. “Propriétés nutritionnelles et fonctionnelles des protéines de tourteaux, de concentrats et d'isolats de Ricinodendron heudelotii (Bail.) Pierre ex Pax et de Tetracarpidium conophorum (Müll. Arg.) : Nutritional and functional properties of proteins from defatted flours, concentrates and isolates of Ricinodendron heudelotii (Bail.) Pierre ex Pax and Tetracarpidium conophorum (Müll. Arg).” 2010. Web. 05 Mar 2021.

Vancouver:

Mezajoug Kenfack LB. Propriétés nutritionnelles et fonctionnelles des protéines de tourteaux, de concentrats et d'isolats de Ricinodendron heudelotii (Bail.) Pierre ex Pax et de Tetracarpidium conophorum (Müll. Arg.) : Nutritional and functional properties of proteins from defatted flours, concentrates and isolates of Ricinodendron heudelotii (Bail.) Pierre ex Pax and Tetracarpidium conophorum (Müll. Arg). [Internet] [Doctoral dissertation]. Vandoeuvre-les-Nancy, INPL; Université de Ngaoundéré – ENSAI - Cameroun; 2010. [cited 2021 Mar 05]. Available from: http://www.theses.fr/2010INPL013N.

Council of Science Editors:

Mezajoug Kenfack LB. Propriétés nutritionnelles et fonctionnelles des protéines de tourteaux, de concentrats et d'isolats de Ricinodendron heudelotii (Bail.) Pierre ex Pax et de Tetracarpidium conophorum (Müll. Arg.) : Nutritional and functional properties of proteins from defatted flours, concentrates and isolates of Ricinodendron heudelotii (Bail.) Pierre ex Pax and Tetracarpidium conophorum (Müll. Arg). [Doctoral Dissertation]. Vandoeuvre-les-Nancy, INPL; Université de Ngaoundéré – ENSAI - Cameroun; 2010. Available from: http://www.theses.fr/2010INPL013N

9. Lemos, Sara Sofia de Campos Pereira. CD8 T cell differentiation during immune responses : Différentiation des cellules T CD8 pendant la réponse immunitaire.

Degree: Docteur es, Immunologie, 2014, Université Paris Descartes – Paris V

URL: http://www.theses.fr/2014PA05T009

► Les lymphocytes T CD8 ont un rôle essentiel dans la protection contre les agents pathogènes intracellulaires et la progression tumorale. Ainsi, la compréhension de la… (more)

▼ Les lymphocytes T CD8 ont un rôle essentiel dans la protection contre les agents pathogènes intracellulaires et la progression tumorale. Ainsi, la compréhension de la diversité des mécanismes de différenciation des lymphocytes T CD8 naïfs en cellules effectrices, ainsi qu’en cellules mémoires compétentes, est fondamentale pour le développement efficace de vaccins à cellules T. Dans ce travail de thèse, nous avons abordé deux questions centrales : (1)Très tôt après l’activation des cellules T CD8, quels sont les mécanismes par lesquels les cellules T effectrices agissent comme effecteurs pro-inflammatoires en recrutant d’autres cellules? Et quel est leur rôle dans la réponse immunitaire? (2) Quel est le rôle du contexte infectieux dans le programme de différenciation des lymphocytes T CD8 ? Est-il responsable de l’hétérogénéité des cellules répondeuses et a-t-il un rôle dans les différents effets protecteurs des cellules mémoires? Afin de répondre à ces questions, nous avons choisit d’utiliser des cellules T CD8 exprimant un récepteur pour l’antigène transgéniques (TCR-Tg) pour suivre la différentiation in vivo des lymphocytes T CD8. De plus, la méthode de RT-PCR sur des séries de cellules uniques, nous a permis d’analyser la co-expression des ARNm dans ces cellules. Comme l’utilisation à haute fréquence de cellules TCR-Tg a été fortement critiquée, nous avons comparé la différenciation de ces cellules avec celle des cellules endogènes (non transgéniques et rares). Dans ce premier manuscrit nous avons observé un comportement similaire, ce qui a renforcé l'avantage d'utiliser des cellules TCR Tg pour étudier les réponses immunitaires des lymphocytes T CD8. De plus, nous avons conclu que la diversité des réponses immunitaires des lymphocytes T CD8 n’est pas conditionnée par la fréquence de cellules naïves. Dans un deuxième manuscrit, nous avons comparé la réponse des cellules OT1 TCR-Tg (spécifiques de l’antigène OVA) à l'infection bactérienne LM-OVA (Listeria Monocytogènes exprimant OVA) avec la réponse des cellules P14 TCR-Tg (spécifiques de l’épitope GP33) à l’infection par le virus LCMV. Nous avons montré que les cellules OT1, stimulées par l’OVA dans un contexte bactérien (LM-OVA), présentent un profil d’expression génique distinct de celui des cellules P14 stimulées par le GP33 dans un contexte viral (LCMV). Nous avons également co-stimulé les cellules P14 et OT1 dans une même souris suivant le même contexte bactérien avec LM-GP33 et LM-OVA. Dans ce cas, nous n’avons pas observé de différence dans le profil d’expression génique. L’ensemble des résultats démontrent que les stimulations spécifiques des cellules T CD8 par différents agents pathogènes génèrent des cellules T CD8 présentant des caractéristiques différentes qui ne sont pas déterminées par la spécificité du TCR mais plutôt par le contexte infectieux. De plus, nous avons montré que les cellules mémoires endogènes résultant de la stimulation des CD8 en présence de LCMV ont été plus efficaces après une deuxième réponse immunitaire que des cellules mémoires… Advisors/Committee Members: Rocha, Benedita (thesis director).

Subjects/Keywords: Cellule T CD8; Différentiation; Effectrice; Inflammatoire; Mémoire; In vivo; Listeria Monocytogenes; LCMV; Cellules TCR-Transgénique; CD8 T cell; Differentiation; Effector; Inflammatory; Memory; In vivo; Listeria Monocytogenes; LCMV; TCR-Transgenic cells; 571.96

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APA (6^th Edition):

Lemos, S. S. d. C. P. (2014). CD8 T cell differentiation during immune responses : Différentiation des cellules T CD8 pendant la réponse immunitaire. (Doctoral Dissertation). Université Paris Descartes – Paris V. Retrieved from http://www.theses.fr/2014PA05T009

Chicago Manual of Style (16^th Edition):

Lemos, Sara Sofia de Campos Pereira. “CD8 T cell differentiation during immune responses : Différentiation des cellules T CD8 pendant la réponse immunitaire.” 2014. Doctoral Dissertation, Université Paris Descartes – Paris V. Accessed March 05, 2021. http://www.theses.fr/2014PA05T009.

MLA Handbook (7^th Edition):

Lemos, Sara Sofia de Campos Pereira. “CD8 T cell differentiation during immune responses : Différentiation des cellules T CD8 pendant la réponse immunitaire.” 2014. Web. 05 Mar 2021.

Vancouver:

Lemos SSdCP. CD8 T cell differentiation during immune responses : Différentiation des cellules T CD8 pendant la réponse immunitaire. [Internet] [Doctoral dissertation]. Université Paris Descartes – Paris V; 2014. [cited 2021 Mar 05]. Available from: http://www.theses.fr/2014PA05T009.

Council of Science Editors:

Lemos SSdCP. CD8 T cell differentiation during immune responses : Différentiation des cellules T CD8 pendant la réponse immunitaire. [Doctoral Dissertation]. Université Paris Descartes – Paris V; 2014. Available from: http://www.theses.fr/2014PA05T009

Utah State University

10. Gebre, Makda. Comparison of Chikungunya Virus Strains in Disease Severity and Susceptibility to T-705 (Favipiravir), In vitro and In vivo.

Degree: MS, Animal, Dairy, and Veterinary Sciences, 2017, Utah State University

URL: https://digitalcommons.usu.edu/etd/6398

► Chikungunya is a mosquito-transmitted disease caused by Chikungunya virus (CHIKV). Symptoms of Chikungunya include debilitating joint pain and swelling, fever and rash. CHIKV was… (more)

Subjects/Keywords: Comparison of Chikungunya Virus Strains in Disease; Severity and Susceptibility to T-705 (Favipiravir); In Vitro; In vivo; Animal Diseases; Animal Sciences; Biology; Virus Diseases

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APA (6^th Edition):

Gebre, M. (2017). Comparison of Chikungunya Virus Strains in Disease Severity and Susceptibility to T-705 (Favipiravir), In vitro and In vivo. (Masters Thesis). Utah State University. Retrieved from https://digitalcommons.usu.edu/etd/6398

Chicago Manual of Style (16^th Edition):

Gebre, Makda. “Comparison of Chikungunya Virus Strains in Disease Severity and Susceptibility to T-705 (Favipiravir), In vitro and In vivo.” 2017. Masters Thesis, Utah State University. Accessed March 05, 2021. https://digitalcommons.usu.edu/etd/6398.

MLA Handbook (7^th Edition):

Gebre, Makda. “Comparison of Chikungunya Virus Strains in Disease Severity and Susceptibility to T-705 (Favipiravir), In vitro and In vivo.” 2017. Web. 05 Mar 2021.

Vancouver:

Gebre M. Comparison of Chikungunya Virus Strains in Disease Severity and Susceptibility to T-705 (Favipiravir), In vitro and In vivo. [Internet] [Masters thesis]. Utah State University; 2017. [cited 2021 Mar 05]. Available from: https://digitalcommons.usu.edu/etd/6398.

Council of Science Editors:

Gebre M. Comparison of Chikungunya Virus Strains in Disease Severity and Susceptibility to T-705 (Favipiravir), In vitro and In vivo. [Masters Thesis]. Utah State University; 2017. Available from: https://digitalcommons.usu.edu/etd/6398

11. Halilaj, Eni. Image-Based Analysis of Thumb Carpometacarpal Joint Biomechanics in Health and Early Osteoarthritis.

Degree: PhD, Biomedical Engineering, 2015, Brown University

URL: https://repository.library.brown.edu/studio/item/bdr:419393/

► Humans have a unique opposable thumb that maintains a fine balance between mobility and stability to allow motions that span from precision handling to power… (more)

▼ Humans have a unique opposable thumb that maintains a fine balance between mobility and stability to allow motions that span from precision handling to power grasping. Much of the versatility of the thumb is due to the saddle geometry of first carpometacarpal (CMC) joint. Located at the base of the thumb, the CMC joint is the most common site of reconstructive surgery in the upper extremity. Nearly 40% of postmenopausal women are affected by CMC osteoarthritis (OA)—a progressive degenerative disease of articular cartilage, bone, and synovium that results in decreased dexterity and partial impairment of the upper extremity. Although current treatment options can alleviate pain, they cannot restore the lost strength and mobility, or halt disease progression, because the pathophysiology of the disease is poorly understood. While both biological and mechanical factors are implicated in the etiology of CMC OA, the synergistic mechanism of disease evolution remains unclear, partially due to a paucity of published data on CMC joint biomechanics in healthy and diseased populations. Accordingly, the objective of this work was to study CMC joint biomechanics using in vivo image-based techniques, and to determine if there are any differences with sex, age, or OA onset that could point to the etiology of the disease. 155 subjects—healthy men and women of two age groups and patients with early stage CMC OA—were recruited for the study. Sequential computed tomography scans of the hands and wrists of all the participants were acquired while they performed functional tasks and range-of-motion activities. The imaging data were used to study joint motion, stability, contact mechanics, articular shape and congruence, as well as to model soft tissue: cartilage and ligaments. The findings presented here suggest that there may not be a mechanical predisposition to CMC OA, but that women may be more susceptible to cartilage loss due to systemic changes with aging. In addition to channeling future research in the right direction, the furthered understanding of CMC joint biomechanics should serve to refine the current surgical procedures and to inspire new conservative options for disease treatment and prevention. Advisors/Committee Members: Crisco, Joseph (Director), Laidlaw, David (Director), Sharon, Swartz (Reader), Braden, Flemming (Reader), Amy, Ladd (Reader).

Subjects/Keywords: in vivo joint kinematics

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APA (6^th Edition):

Halilaj, E. (2015). Image-Based Analysis of Thumb Carpometacarpal Joint Biomechanics in Health and Early Osteoarthritis. (Doctoral Dissertation). Brown University. Retrieved from https://repository.library.brown.edu/studio/item/bdr:419393/

Chicago Manual of Style (16^th Edition):

Halilaj, Eni. “Image-Based Analysis of Thumb Carpometacarpal Joint Biomechanics in Health and Early Osteoarthritis.” 2015. Doctoral Dissertation, Brown University. Accessed March 05, 2021. https://repository.library.brown.edu/studio/item/bdr:419393/.

MLA Handbook (7^th Edition):

Halilaj, Eni. “Image-Based Analysis of Thumb Carpometacarpal Joint Biomechanics in Health and Early Osteoarthritis.” 2015. Web. 05 Mar 2021.

Vancouver:

Halilaj E. Image-Based Analysis of Thumb Carpometacarpal Joint Biomechanics in Health and Early Osteoarthritis. [Internet] [Doctoral dissertation]. Brown University; 2015. [cited 2021 Mar 05]. Available from: https://repository.library.brown.edu/studio/item/bdr:419393/.

Council of Science Editors:

Halilaj E. Image-Based Analysis of Thumb Carpometacarpal Joint Biomechanics in Health and Early Osteoarthritis. [Doctoral Dissertation]. Brown University; 2015. Available from: https://repository.library.brown.edu/studio/item/bdr:419393/

University of Arizona

12. Nguyen, Christopher David. Light Sheet Reflectance Microscopy .

Degree: 2019, University of Arizona

URL: http://hdl.handle.net/10150/633125

► Many human diseases are diagnosed by the histopathological analysis of suspicious lesions where cellular morphological changes are examined at microscopic resolution. Reflectance confocal microscopy (RCM)… (more)

Subjects/Keywords: in vivo; light sheet; microscopy

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APA (6^th Edition):

Nguyen, C. D. (2019). Light Sheet Reflectance Microscopy . (Masters Thesis). University of Arizona. Retrieved from http://hdl.handle.net/10150/633125

Chicago Manual of Style (16^th Edition):

Nguyen, Christopher David. “Light Sheet Reflectance Microscopy .” 2019. Masters Thesis, University of Arizona. Accessed March 05, 2021. http://hdl.handle.net/10150/633125.

MLA Handbook (7^th Edition):

Nguyen, Christopher David. “Light Sheet Reflectance Microscopy .” 2019. Web. 05 Mar 2021.

Vancouver:

Nguyen CD. Light Sheet Reflectance Microscopy . [Internet] [Masters thesis]. University of Arizona; 2019. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/10150/633125.

Council of Science Editors:

Nguyen CD. Light Sheet Reflectance Microscopy . [Masters Thesis]. University of Arizona; 2019. Available from: http://hdl.handle.net/10150/633125

13. Risticevic, Sanja. Solid phase microextraction coupled to comprehensive two-dimensional gas chromatography–time-of-flight mass spectrometry for metabolite profiling of apples: Potential of non-invasive in vivo sampling assay in characterization of metabolome.

Degree: 2012, University of Waterloo

URL: http://hdl.handle.net/10012/7006

► The objective of the current research project relies on implementation of solvent-free, green and environmentally friendly solid phase microextraction (SPME) sample preparation alternative in the… (more)

▼ The objective of the current research project relies on implementation of solvent-free, green and environmentally friendly solid phase microextraction (SPME) sample preparation alternative in the area of complex sample characterization. The advantages that the technique offers in comparison to traditional methods of sample preparation including solvent-free implementation, short sample preparation times, small sample amount requirements, advanced automation capability and minimization of matrix effects are effectively employed during ex vivo and laboratory investigations of complex samples. More important, the underlying features of the technique including miniaturized format, nonexhaustive extraction recoveries and on-site compatibility were fully exploited in order to investigate the metabolome of biological systems directly on the site. Hence, in vivo SPME extraction format was employed in direct immersion SPME sampling of biological systems, hence eliminating the crucial prerequisites associated with multiple preparative steps and incorporation of metabolism quenching that are encountered during implementation of traditional sample preparation methods in global metabolite analysis. Furthermore, in vivo sampling format was hyphenated to comprehensive two-dimensional gas chromatography – time-of-flight mass spectrometry (GCxGC-ToFMS) for high-resolution sampling of volatile and semivolatile metabolites in ‘Honeycrisp’ apples. The initial stages of the project involved evaluation of performance characteristics of commercial SPME extraction coatings in terms of extraction selectivity, extraction sensitivity and desorption efficiency by employing headspace SPME analysis of both aqueous standards spiked with representative volatile and semivolatile metabolites as well as the apple homogenate. DVB/CAR/PDMS coating was selected on the basis of optimum metabolite coverage and extraction sensitivity and was consequently employed during ex vivo and in vivo sampling assays corresponding to determination of volatile and semivolatile metabolites. The former extraction methodology incorporated appropriate sample preparation steps for quenching metabolic activity so that the relevant metabolome profile is not biased against unstable metabolites and those that are susceptible to inter-metabolite conversions which adversely impact preservation of metabolite identity. The two sample preparation assays were compared in terms of metabolite coverage and analytical precision in order to identify SPME route toward characterization of more representative metabolome and determination of instantaneous and more ‘true’ metabolism snapshoot. This is the first report illustrating the implementation of in vivo direct immersion SPME assay for non invasive determination of endogenous fruit metabolites whose profiles and contents are highly correlated to a multitude of influential fruit quality traits.

Subjects/Keywords: solid phase microextraction; in vivo

11 more images…

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Risticevic, S. (2012). Solid phase microextraction coupled to comprehensive two-dimensional gas chromatography–time-of-flight mass spectrometry for metabolite profiling of apples: Potential of non-invasive in vivo sampling assay in characterization of metabolome. (Thesis). University of Waterloo. Retrieved from http://hdl.handle.net/10012/7006

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Risticevic, Sanja. “Solid phase microextraction coupled to comprehensive two-dimensional gas chromatography–time-of-flight mass spectrometry for metabolite profiling of apples: Potential of non-invasive in vivo sampling assay in characterization of metabolome.” 2012. Thesis, University of Waterloo. Accessed March 05, 2021. http://hdl.handle.net/10012/7006.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Risticevic, Sanja. “Solid phase microextraction coupled to comprehensive two-dimensional gas chromatography–time-of-flight mass spectrometry for metabolite profiling of apples: Potential of non-invasive in vivo sampling assay in characterization of metabolome.” 2012. Web. 05 Mar 2021.

Vancouver:

Risticevic S. Solid phase microextraction coupled to comprehensive two-dimensional gas chromatography–time-of-flight mass spectrometry for metabolite profiling of apples: Potential of non-invasive in vivo sampling assay in characterization of metabolome. [Internet] [Thesis]. University of Waterloo; 2012. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/10012/7006.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Risticevic S. Solid phase microextraction coupled to comprehensive two-dimensional gas chromatography–time-of-flight mass spectrometry for metabolite profiling of apples: Potential of non-invasive in vivo sampling assay in characterization of metabolome. [Thesis]. University of Waterloo; 2012. Available from: http://hdl.handle.net/10012/7006

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Louisiana State University

14. Morris, Bart Alan. An Efficient Approach to EPID Transit Dosimetry.

Degree: MS, Physical Sciences and Mathematics, 2014, Louisiana State University

URL: etd-06192014-080930 ; https://digitalcommons.lsu.edu/gradschool_theses/3219

► Introduction: In order to maintain uniform standards in the accuracy of fractionated radiation therapy, quantification of the delivered dose per fraction accuracy is required. The… (more)

▼ Introduction: In order to maintain uniform standards in the accuracy of fractionated radiation therapy, quantification of the delivered dose per fraction accuracy is required. The pupose of this study was to investigate the feasibility of a transit dosimetry method using the electronic portal imaging device (EPID) for dose delivery error detection and prevention. Methods and Materials: In the proposed method, 2D predicted transit images were generated for comparison with online images acquired during treatment. Predicted transit images were generated by convolving through-air EPID measurements of each field with pixel-specific kernels selected from a library of pre-calculated Monte Carlo pencil kernels of various radiological thicknesses. The kernel used for each pixel was selected based on the calculated radiological thickness of the patient along the line joining the pixel to the virtual source. The accuracy of the technique was evaluated in homogeneous plastic water phantoms, a heterogeneous cylindrical phantom, and an anthropomorphic head phantom. Gamma analysis was used to quantify the accuracy of the technique for the various cases. Results: In the comparison between the measured and predicted images, an average of 99.4% of the points in passed a 3%/ 3 mm gamma for the homogeneous plastic water phantoms. Points for the heterogeneous cylindrical phantom analysis had a 94.6% passing rate. For the anthropomorphic head phantom, an average of 98.3% and 96.6% of points passed the 5%/3mm and 3%/3mm gamma criteria, respectively for all field sizes. Failures occurred typically at points when object thickness was changing rapidly or at boundaries between materials, and at the edges of large fields. Discussion: The results suggested that the proposed transit dosimetry method is a feasible approach to in vivo dose monitoring. The gamma analysis passing rates are within the accuracy needed for transit dosimetry. Future research efforts should include evaluation of the method for more complex treatment techniques and assessment of the sensitivity to changes in EPID or linac hardware, as well as characterization of any dependency the method may have on image ghosting or lag, gantry angle, or long-term stability.

Subjects/Keywords: EPID dosimetry transit in vivo

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Morris, B. A. (2014). An Efficient Approach to EPID Transit Dosimetry. (Masters Thesis). Louisiana State University. Retrieved from etd-06192014-080930 ; https://digitalcommons.lsu.edu/gradschool_theses/3219

Chicago Manual of Style (16^th Edition):

Morris, Bart Alan. “An Efficient Approach to EPID Transit Dosimetry.” 2014. Masters Thesis, Louisiana State University. Accessed March 05, 2021. etd-06192014-080930 ; https://digitalcommons.lsu.edu/gradschool_theses/3219.

MLA Handbook (7^th Edition):

Morris, Bart Alan. “An Efficient Approach to EPID Transit Dosimetry.” 2014. Web. 05 Mar 2021.

Vancouver:

Morris BA. An Efficient Approach to EPID Transit Dosimetry. [Internet] [Masters thesis]. Louisiana State University; 2014. [cited 2021 Mar 05]. Available from: etd-06192014-080930 ; https://digitalcommons.lsu.edu/gradschool_theses/3219.

Council of Science Editors:

Morris BA. An Efficient Approach to EPID Transit Dosimetry. [Masters Thesis]. Louisiana State University; 2014. Available from: etd-06192014-080930 ; https://digitalcommons.lsu.edu/gradschool_theses/3219

McGill University

15. Athlan, Eric S. Study of In Vivo and In Vitro Associations Between Neuronal Intermediate Filament Proteins.

Degree: PhD, Department of Biochemistry, 1998, McGill University

URL: https://escholarship.mcgill.ca/downloads/c821gn513.pdf ; https://escholarship.mcgill.ca/concern/theses/sf2687732

►

L'expression des trois protéines de neurofilaments (NF) NFL, NFM et NFH ainsi que I'a-intemexine et la périphérine a été déterminée par immunobuvardage "Western" durant Ie… (more)

▼

L'expression des trois protéines de neurofilaments (NF) NFL, NFM et NFH ainsi que I'a-intemexine et la périphérine a été déterminée par immunobuvardage "Western" durant Ie développement de neurones embryonnaires de ganglions dorsaux en culture. Au jour 0 de la culture, les neurones contenaient surtout de I'a-intemexine. La quantité de périphérine a augmenté de façon significative après 1-2 jours en culture, celle de NFL et NFM après 2-3 jours, et celle de NFH après 5-6 jours. L'analyse des cultures par immunofluorescence indirecte a révélé que les cinq protéines de filament intermédiaire neuronaux (Fin) étaient co-Iocalisées dans tout les compartiments des neurones. L'analyse d'extraits cellulaires solubles au Triton X-100 suite à un traitement a I'acide okadaïque a indiqué que la solubilisation de I'a-intemexine et de la périphérine suivaient Ie même profile que pour NFL, NFM et NFH. L'incubation de ces extraits cellulaires avec des anticorps spécifiques pour chaque protéine permit la co-immunoprécipitation de NFH avec NFL, NFM avec NFL, NFM avec a-internexine et a-internexine avec peripherine démontrant ainsi que ces cinq protéines sont associées dans Ie réseau de Fin des neurones sensorielles en culture.

The developmental profile of the neurofilament (NF) triplet proteins, a-internexin and peripherin in cultured embryonal dorsal root ganglion neurons was determined by Western blot analysis. At day 0 in culture, the neurons contained mostly a-internexin. A significant increase in peripherin levels was seen at days 1-2, in the mid-sized (NFM) and low molecular weight (NFL) NF subunits at days 2-3, and in the high molecular weight (NFH) NF subunit at days 5-6. Immunofluorescence microscopy showed that the five neuronal intermediate filament (nlF) proteins were colocalized in all neuronal cell bodies and neurites. Analysis of Triton X-100-soluble extracts from okadaic acid-treated DRG cultures revealed that peripherin and a-internexin followed the same fragmentation pattern observed for NFs. When these extracts were incubated with antibodies specific for the individual proteins, coimmunoprecipitation of NFH with NFL, NFM with NFL, NFM with a-internexin, and a-internexin with peripherin were observed, demonstrating that nlF proteins in cultured sensory neurons form a highly integrated network.

Advisors/Committee Members: Mushynski, Walter.

Subjects/Keywords: In Vivo

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Athlan, E. S. (1998). Study of In Vivo and In Vitro Associations Between Neuronal Intermediate Filament Proteins. (Doctoral Dissertation). McGill University. Retrieved from https://escholarship.mcgill.ca/downloads/c821gn513.pdf ; https://escholarship.mcgill.ca/concern/theses/sf2687732

Chicago Manual of Style (16^th Edition):

Athlan, Eric S. “Study of In Vivo and In Vitro Associations Between Neuronal Intermediate Filament Proteins.” 1998. Doctoral Dissertation, McGill University. Accessed March 05, 2021. https://escholarship.mcgill.ca/downloads/c821gn513.pdf ; https://escholarship.mcgill.ca/concern/theses/sf2687732.

MLA Handbook (7^th Edition):

Athlan, Eric S. “Study of In Vivo and In Vitro Associations Between Neuronal Intermediate Filament Proteins.” 1998. Web. 05 Mar 2021.

Vancouver:

Athlan ES. Study of In Vivo and In Vitro Associations Between Neuronal Intermediate Filament Proteins. [Internet] [Doctoral dissertation]. McGill University; 1998. [cited 2021 Mar 05]. Available from: https://escholarship.mcgill.ca/downloads/c821gn513.pdf ; https://escholarship.mcgill.ca/concern/theses/sf2687732.

Council of Science Editors:

Athlan ES. Study of In Vivo and In Vitro Associations Between Neuronal Intermediate Filament Proteins. [Doctoral Dissertation]. McGill University; 1998. Available from: https://escholarship.mcgill.ca/downloads/c821gn513.pdf ; https://escholarship.mcgill.ca/concern/theses/sf2687732

University of Georgia

16. Ross, Teresa Adrianna Coleman. In vivo analysis of wild-type and variant human ferrochelatases.

Degree: 2014, University of Georgia

URL: http://hdl.handle.net/10724/24481

► Ferrochelatase is the terminal enzyme in the heme biosynthetic pathway and catalyzes the insertion of ferrous iron into protoporphyrin IX in the formation of protoheme… (more)

Subjects/Keywords: Ferrochelatase; S. cerevisiae; in vivo

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ross, T. A. C. (2014). In vivo analysis of wild-type and variant human ferrochelatases. (Thesis). University of Georgia. Retrieved from http://hdl.handle.net/10724/24481

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ross, Teresa Adrianna Coleman. “In vivo analysis of wild-type and variant human ferrochelatases.” 2014. Thesis, University of Georgia. Accessed March 05, 2021. http://hdl.handle.net/10724/24481.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ross, Teresa Adrianna Coleman. “In vivo analysis of wild-type and variant human ferrochelatases.” 2014. Web. 05 Mar 2021.

Vancouver:

Ross TAC. In vivo analysis of wild-type and variant human ferrochelatases. [Internet] [Thesis]. University of Georgia; 2014. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/10724/24481.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ross TAC. In vivo analysis of wild-type and variant human ferrochelatases. [Thesis]. University of Georgia; 2014. Available from: http://hdl.handle.net/10724/24481

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of New South Wales

17. Gupta, Bakul. Bioresponsive porous silicon photonic crystals for monitoring protease activity in vivo.

Degree: Chemistry, 2013, University of New South Wales

URL: http://handle.unsw.edu.au/1959.4/53296 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:11991/SOURCE02?view=true

► Porous silicon (PSi) photonic crystals have aroused interest as chemical and biological sensing devices and have been used as label-free optical biosensors in recent years.… (more)

▼ Porous silicon (PSi) photonic crystals have aroused interest as chemical and biological sensing devices and have been used as label-free optical biosensors in recent years. The advantage of using PSi as a sensor comes from the ease of fabrication and high quality optics. The recent advances in modifying the surface of these porous materials with different surface chemistries have been utilized to add stability to the otherwise unstable freshly etched PSi surface. The results presented in this thesis have been obtained while developing porous silicon microsensors for their use in detecting protease enzyme activity in vivo. The research presented here primarily focuses on the additions made to the surface modification strategies to add functionalities to the surface for specific target (enzyme) detection and quantification both in vitro and in vivo. A specific class of photonic crystals, rugate filters were fabricated on p-type silicon and used as transducers to transform biological changes to easily read-out optical signals. The presence of a high reflectivity resonant stop-band in the reflectance spectra of these filters made them an ideal choice to be used as a transducing element thereby adding sensitivity to the biosensor. A chemical route based on hydrosilylation of alkynes was adopted to modify the freshly etched PSi surface. This was followed by the copper (I) - catalysed azide cycloaddition reaction using a synthetic anti-fouling polymer to resist non-specific adsorption of biomolecules. Immobilisation of enzyme responsive peptides to these organic layers added specificity to the PSi structure. This specificity was demonstrated by the selective detection of matrix metalloproteases (MMP), MMP-2 and MMP-9 enzymes released from different primary ocular cells by measuring optical blue shifts of these PSi rugate filters. Detection of pg quantities of these MMPs was achieved using these microsensors. The sensitivity achieved by the PSi microsensors was lower than that achieved with zymography; the standard protease detection technique. This functionalisation of the surface with peptides adds versatility to the surface and allows for a range of other biological species to be detected for applications in biology and medicine. Towards protease detection in vivo, PSi microparticles were injected into eyes of live rabbit animal models. The microparticles were shown to be biocompatible for up to 4 weeks. The animal models were injected with a bacterial endotoxin, lipopolysaccharide to cause endotoxin induced uveitis (EIU) with associated up regulation of the release of proteases inside the eye. Efforts were made to detect the release of MMP-9 in vivo by the injected PSi microparticles by measuring optical changes in the reflectivity spectra of these particles in real time. Digestion of the peptide was detected down to 1.3 picomoles (11.7 nM) of the protease in the uveitis infected eye. The detection of proteases both in vitro and in vivo via the use of robust, selective and flexible surface chemistry on PSi photonic… Advisors/Committee Members: Gooding, Prof. J. Justin, Chemistry, Faculty of Science, UNSW, Reece, Dr. Peter J., Physics, Faculty of Science, UNSW.

Subjects/Keywords: In vivo; Porous silicon; Protease

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Gupta, B. (2013). Bioresponsive porous silicon photonic crystals for monitoring protease activity in vivo. (Doctoral Dissertation). University of New South Wales. Retrieved from http://handle.unsw.edu.au/1959.4/53296 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:11991/SOURCE02?view=true

Chicago Manual of Style (16^th Edition):

Gupta, Bakul. “Bioresponsive porous silicon photonic crystals for monitoring protease activity in vivo.” 2013. Doctoral Dissertation, University of New South Wales. Accessed March 05, 2021. http://handle.unsw.edu.au/1959.4/53296 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:11991/SOURCE02?view=true.

MLA Handbook (7^th Edition):

Gupta, Bakul. “Bioresponsive porous silicon photonic crystals for monitoring protease activity in vivo.” 2013. Web. 05 Mar 2021.

Vancouver:

Gupta B. Bioresponsive porous silicon photonic crystals for monitoring protease activity in vivo. [Internet] [Doctoral dissertation]. University of New South Wales; 2013. [cited 2021 Mar 05]. Available from: http://handle.unsw.edu.au/1959.4/53296 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:11991/SOURCE02?view=true.

Council of Science Editors:

Gupta B. Bioresponsive porous silicon photonic crystals for monitoring protease activity in vivo. [Doctoral Dissertation]. University of New South Wales; 2013. Available from: http://handle.unsw.edu.au/1959.4/53296 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:11991/SOURCE02?view=true

University of Manchester

18. Harrison, James Andrew Roy. Utility of hepatocellular systems to measure drug transport and metabolism for prediction of in vivo drug clearance.

Degree: 2018, University of Manchester

URL: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:313017

► Predictive pharmacokinetics now forms a critical part of the drug discovery process. However, metabolic data has been demonstrated to under-predict in vivo clearance, while no… (more)

▼ Predictive pharmacokinetics now forms a critical part of the drug discovery process. However, metabolic data has been demonstrated to under-predict in vivo clearance, while no large scale analysis has been performed for hepatic uptake data. The primary aim of this thesis was therefore to investigate the utility of various clearance parameters generated in hepatocellular assays for the prediction of in vivo clearance. Large scale literature analyses were performed for uptake data in both rat and human hepatocytes. In the rat, it was highlighted that over-prediction was the predominant issue for suspension and media loss hepatocyte assays. Conversely, monolayer and SCH assays suffered from under-prediction. However, in human hepatocytes under-prediction was observed in all assay formats. Use of empirical scaling factors improved predictions in both species, and are recommended for future use. The media loss assay, a method described by Soars et al[1], was further developed in rat hepatocytes through inclusion of transporter and metabolic enzyme inhibitors. Using a two-compartment model, individual clearance parameters (CLmet, CLactive and CLpassive) were estimated, and were also used to estimate binding and partitioning terms (Kp, Kpu and fucell). IVIVE of data produced from this assay resulted in a lower bias than had been noted from literature data. However, it was hypothesised that additional clearance parameters could be used in a mechanistic approach to further improve predictions. SCH assays were performed to generate estimates of uptake rates, as well as efflux rates from both the sinusoidal and canalicular membranes. Combining clearance terms from both the media loss and SCH assays using the CLint,total term led to less bias when predicting in vivo clearance than observed using uptake or metabolism data alone. Additionally, the use of empirical scaling factors identified from the literature analysis led to further reduction in prediction bias. Future work must now focus on the application of this research to human hepatocytes. It is concluded that the work presented in this thesis provides evidence for the usefulness of both uptake and extended clearance terms, in conjunction with empirical scaling methods, for the prediction of in vivo clearance. Adaptation of the media loss assay allowed the estimation of several key pharmacokinetic parameters. Although some of these are not always useful in a quantitative fashion, they remain essential properties of a compound that must be considered when predicting behaviour within the body. Advisors/Committee Members: Houston, James.

Subjects/Keywords: Hepatocytes; Pharmacokinetics; In vivo prediction

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Harrison, J. A. R. (2018). Utility of hepatocellular systems to measure drug transport and metabolism for prediction of in vivo drug clearance. (Doctoral Dissertation). University of Manchester. Retrieved from http://www.manchester.ac.uk/escholar/uk-ac-man-scw:313017

Chicago Manual of Style (16^th Edition):

Harrison, James Andrew Roy. “Utility of hepatocellular systems to measure drug transport and metabolism for prediction of in vivo drug clearance.” 2018. Doctoral Dissertation, University of Manchester. Accessed March 05, 2021. http://www.manchester.ac.uk/escholar/uk-ac-man-scw:313017.

MLA Handbook (7^th Edition):

Harrison, James Andrew Roy. “Utility of hepatocellular systems to measure drug transport and metabolism for prediction of in vivo drug clearance.” 2018. Web. 05 Mar 2021.

Vancouver:

Harrison JAR. Utility of hepatocellular systems to measure drug transport and metabolism for prediction of in vivo drug clearance. [Internet] [Doctoral dissertation]. University of Manchester; 2018. [cited 2021 Mar 05]. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:313017.

Council of Science Editors:

Harrison JAR. Utility of hepatocellular systems to measure drug transport and metabolism for prediction of in vivo drug clearance. [Doctoral Dissertation]. University of Manchester; 2018. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:313017

University of Manchester

19. Harrison, James. Utility of hepatocellular systems to measure drug transport and metabolism for prediction of in vivo drug clearance.

Degree: PhD, 2018, University of Manchester

URL: https://www.research.manchester.ac.uk/portal/en/theses/utility-of-hepatocellular-systems-to-measure-drug-transport-and-metabolism-for-prediction-of-in-vivo-drug-clearance(2da57336-1850-47a4-82ab-24e59798283e).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.823108

► Predictive pharmacokinetics now forms a critical part of the drug discovery process. However, metabolic data has been demonstrated to under-predict in vivo clearance, while no… (more)

▼ Predictive pharmacokinetics now forms a critical part of the drug discovery process. However, metabolic data has been demonstrated to under-predict in vivo clearance, while no large scale analysis has been performed for hepatic uptake data. The primary aim of this thesis was therefore to investigate the utility of various clearance parameters generated in hepatocellular assays for the prediction of in vivo clearance. Large scale literature analyses were performed for uptake data in both rat and human hepatocytes. In the rat, it was highlighted that over-prediction was the predominant issue for suspension and media loss hepatocyte assays. Conversely, monolayer and SCH assays suffered from under-prediction. However, in human hepatocytes under-prediction was observed in all assay formats. Use of empirical scaling factors improved predictions in both species, and are recommended for future use. The media loss assay, a method described by Soars et al[1], was further developed in rat hepatocytes through inclusion of transporter and metabolic enzyme inhibitors. Using a two-compartment model, individual clearance parameters (CLmet, CLactive and CLpassive) were estimated, and were also used to estimate binding and partitioning terms (Kp, Kpu and fucell). IVIVE of data produced from this assay resulted in a lower bias than had been noted from literature data. However, it was hypothesised that additional clearance parameters could be used in a mechanistic approach to further improve predictions. SCH assays were performed to generate estimates of uptake rates, as well as efflux rates from both the sinusoidal and canalicular membranes. Combining clearance terms from both the media loss and SCH assays using the CLint,total term led to less bias when predicting in vivo clearance than observed using uptake or metabolism data alone. Additionally, the use of empirical scaling factors identified from the literature analysis led to further reduction in prediction bias. Future work must now focus on the application of this research to human hepatocytes. It is concluded that the work presented in this thesis provides evidence for the usefulness of both uptake and extended clearance terms, in conjunction with empirical scaling methods, for the prediction of in vivo clearance. Adaptation of the media loss assay allowed the estimation of several key pharmacokinetic parameters. Although some of these are not always useful in a quantitative fashion, they remain essential properties of a compound that must be considered when predicting behaviour within the body.

Subjects/Keywords: Hepatocytes; Pharmacokinetics; In vivo prediction

Record Details Similar Records Cite « Share

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Harrison, J. (2018). Utility of hepatocellular systems to measure drug transport and metabolism for prediction of in vivo drug clearance. (Doctoral Dissertation). University of Manchester. Retrieved from https://www.research.manchester.ac.uk/portal/en/theses/utility-of-hepatocellular-systems-to-measure-drug-transport-and-metabolism-for-prediction-of-in-vivo-drug-clearance(2da57336-1850-47a4-82ab-24e59798283e).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.823108

Chicago Manual of Style (16^th Edition):

Harrison, James. “Utility of hepatocellular systems to measure drug transport and metabolism for prediction of in vivo drug clearance.” 2018. Doctoral Dissertation, University of Manchester. Accessed March 05, 2021. https://www.research.manchester.ac.uk/portal/en/theses/utility-of-hepatocellular-systems-to-measure-drug-transport-and-metabolism-for-prediction-of-in-vivo-drug-clearance(2da57336-1850-47a4-82ab-24e59798283e).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.823108.

MLA Handbook (7^th Edition):

Harrison, James. “Utility of hepatocellular systems to measure drug transport and metabolism for prediction of in vivo drug clearance.” 2018. Web. 05 Mar 2021.

Vancouver:

Harrison J. Utility of hepatocellular systems to measure drug transport and metabolism for prediction of in vivo drug clearance. [Internet] [Doctoral dissertation]. University of Manchester; 2018. [cited 2021 Mar 05]. Available from: https://www.research.manchester.ac.uk/portal/en/theses/utility-of-hepatocellular-systems-to-measure-drug-transport-and-metabolism-for-prediction-of-in-vivo-drug-clearance(2da57336-1850-47a4-82ab-24e59798283e).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.823108.

Council of Science Editors:

Harrison J. Utility of hepatocellular systems to measure drug transport and metabolism for prediction of in vivo drug clearance. [Doctoral Dissertation]. University of Manchester; 2018. Available from: https://www.research.manchester.ac.uk/portal/en/theses/utility-of-hepatocellular-systems-to-measure-drug-transport-and-metabolism-for-prediction-of-in-vivo-drug-clearance(2da57336-1850-47a4-82ab-24e59798283e).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.823108

20. Vautheny, Audrey. Rôle de la neuroinflammation et du récepteur microglial TREM2 dans la progression de deux modèles de tauopathie : Role of Neuroinflammation and the TREM2 Microglial Receptor in the Progression of two Models of Tauopathy.

Degree: Docteur es, Sciences de la vie et de la santé, 2019, Université Paris-Saclay (ComUE)

URL: http://www.theses.fr/2019SACLS170

►

Les processus de neuro-inflammation jouent un rôle majeur dans la maladie d'Alzheimer (MA). Des études génétiques récentes démontrent cette association entre neuro-inflammation et MA et… (more)

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Les processus de neuro-inflammation jouent un rôle majeur dans la maladie d'Alzheimer (MA). Des études génétiques récentes démontrent cette association entre neuro-inflammation et MA et impliquent notamment un gène, TREM2, qui code pour un récepteur exprimé à la surface de la microglie. La tauopathie est une lésion caractéristique de la MA. Elle se traduit par l’hyperphosphorylation et l’agrégation intraneuronale de la protéine Tau. Les travaux sur le rôle de TREM2 dans le développement de la pathologie Tau sont peu nombreux et donnent des résultats contradictoiresAinsi, l’objectif de ma thèse est d’étudier le rôle de la neuroinflammation et de TREM2 dans la progression de la tauopathie, dans deux modèles différents. Le premier est obtenu par injection stéréotaxique de vecteurs AAV dans la couche CA1 de l’hippocampe de souris déficientes ou non en TREM2. Ces vecteurs entrainent la surexpression de différentes formes de la protéine Tau humaine et permettent de récapituler les différents stades de la tauopathie.Nous avons en parallèle utilisé un modèle transgénique plus progressif de tauopathie, la souris THY-Tau22, afin d’étudier l’influence du stade de la pathologie dans l’effet provoqué par une déficience en TREM2 sur l’évolution de la pathologie. Notre étude a mis en évidence la toxicité des formes solubles de Tau dans le modèle AAV par rapport à ses formes agrégées. Le modèle transgénique THY-Tau22 nous a permis de mettre en évidence une augmentation des lésions tauopathiques dans les souris déficientes en TREM2 par rapport aux souris qui ne le sont pas, uniquement à un stade avancé. Cela suggère que, à l’instar des modèles amyloïdes, l’effet de la déficience en TREM2 sur le décours de la tauopathie est différent en fonction du stade considéré.

Neuroinflammation processes appear to play a major role in Alzheimer's disease (AD). Recent genetic studies support this correlation between neuroinflammation and AD and include a gene, TREM2, expressed on microglial surface. Tauopathy is a characteristic lesion of AD. It results in hyperphosphorylation and intraneuronal aggregation of Tau protein. In the literature, only few articles describe the role of TREM2 in the development of Tau pathology, and they report contradictory results. We therefore do not know for sure whether a deficiency in TREM2 has a deleterious effect or not on tauopathy. Thus, the goal of my thesis is to study the role of neuroinflammation and TREM2 in the progression of tauopathy, in two different models. The first is obtained by stereotaxic injection of AAV vectors into the CA1 layer of the hippocampus of TREM2-deficient or non-deficient mice. These vectors lead to the overexpression of different forms of the human tau protein, thus making it possible to recapitulate the different tauopathy stages.In parallel, we used a more progressive trangenic model of tauopathy, the THY-Tau22 mouse, to study the influence of TREM2 deficiency at different stage of the pathology. Our study demonstrated the toxicity of Tau soluble forms in the AAV model compared…

Advisors/Committee Members: Bemelmans, Alexis (thesis director).

Subjects/Keywords: Tauopathie; Neuroinflammation; Modèles in vivo; Tauopathy; Neuroinflammation; In vivo models

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Vautheny, A. (2019). Rôle de la neuroinflammation et du récepteur microglial TREM2 dans la progression de deux modèles de tauopathie : Role of Neuroinflammation and the TREM2 Microglial Receptor in the Progression of two Models of Tauopathy. (Doctoral Dissertation). Université Paris-Saclay (ComUE). Retrieved from http://www.theses.fr/2019SACLS170

Chicago Manual of Style (16^th Edition):

Vautheny, Audrey. “Rôle de la neuroinflammation et du récepteur microglial TREM2 dans la progression de deux modèles de tauopathie : Role of Neuroinflammation and the TREM2 Microglial Receptor in the Progression of two Models of Tauopathy.” 2019. Doctoral Dissertation, Université Paris-Saclay (ComUE). Accessed March 05, 2021. http://www.theses.fr/2019SACLS170.

MLA Handbook (7^th Edition):

Vautheny, Audrey. “Rôle de la neuroinflammation et du récepteur microglial TREM2 dans la progression de deux modèles de tauopathie : Role of Neuroinflammation and the TREM2 Microglial Receptor in the Progression of two Models of Tauopathy.” 2019. Web. 05 Mar 2021.

Vancouver:

Vautheny A. Rôle de la neuroinflammation et du récepteur microglial TREM2 dans la progression de deux modèles de tauopathie : Role of Neuroinflammation and the TREM2 Microglial Receptor in the Progression of two Models of Tauopathy. [Internet] [Doctoral dissertation]. Université Paris-Saclay (ComUE); 2019. [cited 2021 Mar 05]. Available from: http://www.theses.fr/2019SACLS170.

Council of Science Editors:

Vautheny A. Rôle de la neuroinflammation et du récepteur microglial TREM2 dans la progression de deux modèles de tauopathie : Role of Neuroinflammation and the TREM2 Microglial Receptor in the Progression of two Models of Tauopathy. [Doctoral Dissertation]. Université Paris-Saclay (ComUE); 2019. Available from: http://www.theses.fr/2019SACLS170

Uniwersytet im. Adama Mickiewicza w Poznaniu

21. Igielska-Kalwat, Joanna. Badanie wpływu form kosmetycznych na biodostępność wybranych karotenoidów .

Degree: 2016, Uniwersytet im. Adama Mickiewicza w Poznaniu

URL: http://hdl.handle.net/10593/14415

► Skóra stanowi bardzo dobry model badawczy w odniesieniu do całościowego zjawiska starzenia. Jest ona bezpośrednio narażona na destrukcyjne działanie czynników zewnętrznych. Ulega starzeniu szybciej niż… (more)

Subjects/Keywords: karotenoidy; carotenoids; biodostępność; bioavailability; badania in vivo; in vivo studies

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Igielska-Kalwat, J. (2016). Badanie wpływu form kosmetycznych na biodostępność wybranych karotenoidów . (Doctoral Dissertation). Uniwersytet im. Adama Mickiewicza w Poznaniu. Retrieved from http://hdl.handle.net/10593/14415

Chicago Manual of Style (16^th Edition):

Igielska-Kalwat, Joanna. “Badanie wpływu form kosmetycznych na biodostępność wybranych karotenoidów .” 2016. Doctoral Dissertation, Uniwersytet im. Adama Mickiewicza w Poznaniu. Accessed March 05, 2021. http://hdl.handle.net/10593/14415.

MLA Handbook (7^th Edition):

Igielska-Kalwat, Joanna. “Badanie wpływu form kosmetycznych na biodostępność wybranych karotenoidów .” 2016. Web. 05 Mar 2021.

Vancouver:

Igielska-Kalwat J. Badanie wpływu form kosmetycznych na biodostępność wybranych karotenoidów . [Internet] [Doctoral dissertation]. Uniwersytet im. Adama Mickiewicza w Poznaniu; 2016. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/10593/14415.

Council of Science Editors:

Igielska-Kalwat J. Badanie wpływu form kosmetycznych na biodostępność wybranych karotenoidów . [Doctoral Dissertation]. Uniwersytet im. Adama Mickiewicza w Poznaniu; 2016. Available from: http://hdl.handle.net/10593/14415

Université de Montréal

22. Zagriadskaia, Ekaterina. Shape-keeping elements in tRNA.

Degree: 2004, Université de Montréal

URL: http://hdl.handle.net/1866/14778

Subjects/Keywords: Structure d'ARNt; Boucle T; Forme L; Bibliothèque des gènes; Sélection in vivo

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Zagriadskaia, E. (2004). Shape-keeping elements in tRNA. (Thesis). Université de Montréal. Retrieved from http://hdl.handle.net/1866/14778

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Zagriadskaia, Ekaterina. “Shape-keeping elements in tRNA.” 2004. Thesis, Université de Montréal. Accessed March 05, 2021. http://hdl.handle.net/1866/14778.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Zagriadskaia, Ekaterina. “Shape-keeping elements in tRNA.” 2004. Web. 05 Mar 2021.

Vancouver:

Zagriadskaia E. Shape-keeping elements in tRNA. [Internet] [Thesis]. Université de Montréal; 2004. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/1866/14778.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Zagriadskaia E. Shape-keeping elements in tRNA. [Thesis]. Université de Montréal; 2004. Available from: http://hdl.handle.net/1866/14778

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Newcastle

23. Choi, Jeong-Hwa. In vitro and in vivo approaches to the examination of folate-related nutritional genetics in health and disease.

Degree: Food Science, 2014, University of Newcastle

URL: http://hdl.handle.net/1959.13/1041655

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Research Doctorate - PhD (Food Science)

Folic acid has been the focus of extensive research since it plays an essential role in the human body… (more)

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Research Doctorate - PhD (Food Science)

Folic acid has been the focus of extensive research since it plays an essential role in the human body as a major coenzyme in one-carbon metabolism. This metabolic role facilitates both methionine and DNA synthesis. It has been suggested that increased folate intake, and hence blood status, provides benefits in maintaining health. However, a growing body of literature now reports differing opinions with respect to the level of folic acid intake, the type of vitamer and pathoaetiological outcomes. Part of this controversy lies in the study of cancer aetiology, particularly in the context of adenomatous polyp (AP) occurrence. AP is an antecedent of colorectal cancer. As part of the present study an in vivo model was therefore developed in order to re-examine the role of folate in carcinogenesis, taking into account both genetic variants and environmental factors. To obtain further evidence, the biological characteristics of folate vitamers in promoting cancer cell proliferation were examined using an in vitro model. 202 individuals were recruited and placed into two groups depending upon whether they had been diagnosed with AP or were control subjects. The blood folate, thiol levels, dietary folate intake, including synthetic and natural forms, and intake of nutrients related to one-carbon metabolism (pyridoxine, riboflavin, cobalamin, niacin, methionine and vitamin C) of each subject were evaluated. Sixteen major genetic variants in folate metabolism were also determined as potential risk factors in AP aetiology: MTHFR C677T, A1208C, G1793A, SHMT C1420T, TS 1496del6, TSER 2R3R, TS 3RG>C, GCPII C1561T, CBS 844ins66, DHFR 19bp del , RCF G80A, CTH G1364T, IVS 10-430 C>T, BHMT G595A, MS A2756G and MSR A66G. Statistical analysis has provided evidence to suggest that AP aetiology depends upon a range of interactions between genes and nutrition. The following factors were found to be associated with an increased risk of developing AP: RBC folate level (p for females = 0.0021 and males = 0.004), nutritional intake (p for methylfolate intake below the median = 0.0189 and cobalamin intake above median = 0.0402) and genetic variants (p for CBS 844ins68 = 0.035, TS 1496del6 = 0.048, MSR A66G = 0.025 and MTHFR C677T- CBS 844ins66 = 0.0403). Although no direct correlation was found between PteGlu intake and the occurrence of AP, genetic variants predicted a differential AP risk depending on total dietary folate intake level (in subjects with total dietary folate intake above the RDI: p for MTHFR A1298C= 0.026, MTHFR C677T-A1298C-G1793A=0.016, TS 1496del6 = 0.024, DHFR 19bp del = 0.032, and, in subjects with total dietary folate intake below the RDI: MTHFR A1298C2= 0.011, RFC G80A-GCPII C1561T3= 0.033). Additionally, vitamin C was also associated with occurrence of AP when examined by RCF G80A and GCPII C1561T genotype (p for RFC G80A AA = 0.0473, GCP II C1561T CT= 0.0276 and RFC G80A-GCPII C1561T AA/CC = 0.0026). An in vitro model utilizing both colon (Caco-2) and breast cancer (MCF 7)…

Advisors/Committee Members: University of Newcastle. Faculty of Science & Information Technology, School of Environmental and Life Sciences.

Subjects/Keywords: cancer; folic acid; in vitro; in vivo

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Choi, J. (2014). In vitro and in vivo approaches to the examination of folate-related nutritional genetics in health and disease. (Doctoral Dissertation). University of Newcastle. Retrieved from http://hdl.handle.net/1959.13/1041655

Chicago Manual of Style (16^th Edition):

Choi, Jeong-Hwa. “In vitro and in vivo approaches to the examination of folate-related nutritional genetics in health and disease.” 2014. Doctoral Dissertation, University of Newcastle. Accessed March 05, 2021. http://hdl.handle.net/1959.13/1041655.

MLA Handbook (7^th Edition):

Choi, Jeong-Hwa. “In vitro and in vivo approaches to the examination of folate-related nutritional genetics in health and disease.” 2014. Web. 05 Mar 2021.

Vancouver:

Choi J. In vitro and in vivo approaches to the examination of folate-related nutritional genetics in health and disease. [Internet] [Doctoral dissertation]. University of Newcastle; 2014. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/1959.13/1041655.

Council of Science Editors:

Choi J. In vitro and in vivo approaches to the examination of folate-related nutritional genetics in health and disease. [Doctoral Dissertation]. University of Newcastle; 2014. Available from: http://hdl.handle.net/1959.13/1041655

24. Geiza da Graça Leite Rissardi. Efeito do relaxamento de Jacobson modificado, nas medidas de pressão arterial, freqüência cardíaca e freqüência respiratória em pacientes hansenianos.

Degree: 2007, Faculdade de Medicina de São José do Rio Preto

URL: http://bdtd.famerp.br//tde_busca/arquivo.php?codArquivo=184 ; http://bdtd.famerp.br//tde_busca/arquivo.php?codArquivo=290

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A doença de Hansen em face de seus aspectos fisiopatológicos e sociais pode desencadear o estresse favorecendo cronicamente o aparecimento de problemas cardiovasculares. Estresse crônico,… (more)

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A doença de Hansen em face de seus aspectos fisiopatológicos e sociais pode desencadear o estresse favorecendo cronicamente o aparecimento de problemas cardiovasculares. Estresse crônico, pode levar a ocorrência de pressão arterial elevada além de crises de dispnéia e taquicardia Objetivo Avaliar se uma técnica do Relaxamento Muscular Progressivo é eficaz no sentido de reduzir os sinais indicativos de estresse cardiovascular. Método Aplicou-se nesses pacientes, para tratamento psicossomático, uma técnica de relaxamento chamada Relaxamento Muscular Progressivo de Jacobson Modificado, avaliando sua eficácia no que se refere à diminuição das seguintes variáveis: pressão arterial (PA), freqüência cardíaca (FC) e freqüência respiratória.(FR). As terapias se realizaram com 16 pacientes, com idades entre 20 e 75 anos (52,715,9 anos; mediana 56,5 anos), 56% masculinos, duas vezes por semana, com duração de uma hora, por 10 sessões. Todas as medidas foram feitas antes e após as terapias. As sessões eram feitas em grupo de no máximo seis pessoas, com pessoas deitadas em colchonete. Resultados Obteve-se redução significante das medidas da FC (valor-p<0,0001) e FR (valor-p<0,0001), mas não houve alteração da PA (valor-p=0,42 para pressão arterial sistólica e 0,24 para pressão arterial diastólica). Conclusão A terapia usada pode ser considerada eficaz para os pacientes em relação á FC e FR, e quanto à PA, não foi possível detectar redução significante.

The Hansen disease by its physiopatological and social aspects can promote the arising of stress and the development of cardiovascular diseases. The chronic stress causes high arterial systemic pressures. Dyspnea and tachycardia crisis can also occur in stressful situations. Objective To evaluate if the technique of Modified Jacobsons Progressive Muscular Relaxing is effective to reduce the indicative signs of cardiovascular stress. Method Aiming at dealing with those patients through a psychosomatic view, a relaxing technique called Modified Jacobsons Progressive Muscular Relaxing was used to evaluate its efficiency in reducing the following variables: arterial pressure, heart rate (HR) and respiratory rate (RR). The therapies were made with sixteen patients, who were between 20 and 75 years old (52.7 15.9 years old; average 56.6 years old), 56% male, twice a week, with one hour of duration, for ten sections. All the measures were made by only one person, before and after the therapy sections. The sections were made in groups of at most six people, with the people lying on mattresses. Results As a result, a significant reduction of HR (value p<0.0001) and RR (value p<0.0001) was obtained, but there wasnt any modification of the AP (value p=0.42 for Systolic Arterial Pressure and 0.24 for Diastolic Arterial Pressure. Conclusion This relaxing therapy, can be considered efficient to the patients in relation to the HR and RR; and, referring to the AP, it was not possible to detect a significant lowering .

Advisors/Committee Members: Aparecida Maria Catai, Nelson Iguimar Valerio, Moacir Fernandes de Godoy.

Subjects/Keywords: DERMATOLOGIA; Técnica de Relaxamento; Pressão Arterial; Freqüência Cardíaca; Freqüência Respiratória; Estresse; Hanseníase; Tecnicas de Relacionamento; Relaxing Technique; Arterial Pressure; Heart Rate; Respiratory Rate; Stress; Hansens Disease; T?cnicas de Relajaci?n; Relaxation Techiniques; Presi?n Sangu?nea; Blood Pressure; Frecuencia Cardíaca; Estrés; Lepra; Leprosy

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Rissardi, G. d. G. L. (2007). Efeito do relaxamento de Jacobson modificado, nas medidas de pressão arterial, freqüência cardíaca e freqüência respiratória em pacientes hansenianos. (Thesis). Faculdade de Medicina de São José do Rio Preto. Retrieved from http://bdtd.famerp.br//tde_busca/arquivo.php?codArquivo=184 ; http://bdtd.famerp.br//tde_busca/arquivo.php?codArquivo=290

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Rissardi, Geiza da Graça Leite. “Efeito do relaxamento de Jacobson modificado, nas medidas de pressão arterial, freqüência cardíaca e freqüência respiratória em pacientes hansenianos.” 2007. Thesis, Faculdade de Medicina de São José do Rio Preto. Accessed March 05, 2021. http://bdtd.famerp.br//tde_busca/arquivo.php?codArquivo=184 ; http://bdtd.famerp.br//tde_busca/arquivo.php?codArquivo=290.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Rissardi, Geiza da Graça Leite. “Efeito do relaxamento de Jacobson modificado, nas medidas de pressão arterial, freqüência cardíaca e freqüência respiratória em pacientes hansenianos.” 2007. Web. 05 Mar 2021.

Vancouver:

Rissardi GdGL. Efeito do relaxamento de Jacobson modificado, nas medidas de pressão arterial, freqüência cardíaca e freqüência respiratória em pacientes hansenianos. [Internet] [Thesis]. Faculdade de Medicina de São José do Rio Preto; 2007. [cited 2021 Mar 05]. Available from: http://bdtd.famerp.br//tde_busca/arquivo.php?codArquivo=184 ; http://bdtd.famerp.br//tde_busca/arquivo.php?codArquivo=290.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Rissardi GdGL. Efeito do relaxamento de Jacobson modificado, nas medidas de pressão arterial, freqüência cardíaca e freqüência respiratória em pacientes hansenianos. [Thesis]. Faculdade de Medicina de São José do Rio Preto; 2007. Available from: http://bdtd.famerp.br//tde_busca/arquivo.php?codArquivo=184 ; http://bdtd.famerp.br//tde_busca/arquivo.php?codArquivo=290

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

25. Costa, Fernanda Caetano. Segmenta??o sem?ntica de ?reas desmatadas utilizando Redes Neurais Convolucionais no sul da Amaz?nia Legal.

Degree: 2020, Universidade Federal do Amazonas

URL: https://tede.ufam.edu.br/handle/tede/8028

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Neste trabalho propomos a utiliza??o de redes neurais convolucionais para segmenta??o de ?reas desmatadas na regi?o do sul da Amaz?nia Legal em imagens de sat?lite… (more)

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Neste trabalho propomos a utiliza??o de redes neurais convolucionais para segmenta??o de ?reas desmatadas na regi?o do sul da Amaz?nia Legal em imagens de sat?lite Landsat-8. Para realiza??o deste trabalho foi constru?do um banco de imagens mosaico, composto por amostras de ?reas desmatadas e ?reas de floresta extra?das das imagens de sat?lite Landsat- 8, identificando as ?reas desmatadas atrav?s da utiliza??o dos dados de desmatamento do projeto PRODES do INPE. As arquiteturas de redes convolucionais utilizadas foram as propostas no trabalho de Serr?o et al. (2020) e de Miyagawa et al. (2018). O treinamento das redes foi realizado com 32 ?pocas utilizando m?todos de otimiza??o SGDM, RMSProp e ADAM e os m?todos de regulariza??o L2 e Dropout. Combinando as tr?s arquiteturas com esses m?todos, totalizaram 36 simula??es. Para avaliar o desempenho das arquiteturas para segmenta??o das ?reas desmatadas, permitindo uma compara??o entre os modelos, foi escolhida a m?trica acur?cia. Ap?s a avalia??o do desempenho dos modelos no conjunto de valida??o, seis deles foram selecionados para serem avaliados com o conjunto de teste. O modelo que apresentou o melhor resultado, uma acur?cia de 99.97%, foi o que utilizou a seguinte combina??o: CNN2 + RMSProp + Dropout. O resultado deste trabalho foi comparado com os resultados dos trabalhos de Ortega et al. (2019), Adarme et al. (2020) e De Bem et al. (2020) tendo obtido resultados superiores ?queles obtidos por esses autores. Os resultados mostraram que as redes neurais convolucionais s?o capazes de realizar com alto desempenho a tarefa de segmenta??o de ?reas desmatadas

In this work, we propose the evaluation of convolutional neural networks architectures to segment deforested areas in the southern region of the Brazilian Legal Amazon, using Landsat-8 satellite images. In order to carry out this work, a mosaic image data set was elaborated, consisting of samples of deforested areas and forest areas extracted from the Landsat-8 satellite images. The deforested areas were identified through the use of deforestation data from the PRODES project at INPE. The architectures of convolutional networks used in our research were those proposed in the work of Serr?o et al. (2020) and Miyagawa et al. (2018). The training of the networks was carried out over 32 epochs using SGDM, RMSProp and ADAM optimization methods and the L2 and Dropout regularization methods. The combining of the three architectures with these methods, resulting in 36 simulations. To measure the performance of the architectures for segmented deforested region, allowing a comparison between the models, the accuracy metric was chosen. After evaluating the performance of the models in the validation set, six of them were selected to be evaluated with the test set. The model that presented the best result, with an accuracy of 99.97%, was the one that used the following combination: CNN2 + RMSProp + Dropout. The results of this work were compared with the results of the work of Ortega et al.…

Advisors/Committee Members: Costa Filho, C?cero Ferreira Fernandes, http://lattes.cnpq.br/3029011770761387, Pereira, Jos? Raimundo Gomes, http://lattes.cnpq.br/3697983438100904, Xavier, Clahildek Matos, http://lattes.cnpq.br/6870670168555921.

Subjects/Keywords: Redes neurais (Computa??o); Algoritmos computacionais; Sat?lite Landsat-8; Segmenta??o de ?reas desmatadas; Amaz?nia Legal; ENGENHARIAS; ?reas Desmatadas; Redes Neurais Convolucionais; Imagem-Mosaico; Landsat-8; Segmenta??o; Algoritmos de otimiza??o; T?cnicas de regulariza??o

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Costa, F. C. (2020). Segmenta??o sem?ntica de ?reas desmatadas utilizando Redes Neurais Convolucionais no sul da Amaz?nia Legal. (Masters Thesis). Universidade Federal do Amazonas. Retrieved from https://tede.ufam.edu.br/handle/tede/8028

Chicago Manual of Style (16^th Edition):

Costa, Fernanda Caetano. “Segmenta??o sem?ntica de ?reas desmatadas utilizando Redes Neurais Convolucionais no sul da Amaz?nia Legal.” 2020. Masters Thesis, Universidade Federal do Amazonas. Accessed March 05, 2021. https://tede.ufam.edu.br/handle/tede/8028.

MLA Handbook (7^th Edition):

Costa, Fernanda Caetano. “Segmenta??o sem?ntica de ?reas desmatadas utilizando Redes Neurais Convolucionais no sul da Amaz?nia Legal.” 2020. Web. 05 Mar 2021.

Vancouver:

Costa FC. Segmenta??o sem?ntica de ?reas desmatadas utilizando Redes Neurais Convolucionais no sul da Amaz?nia Legal. [Internet] [Masters thesis]. Universidade Federal do Amazonas; 2020. [cited 2021 Mar 05]. Available from: https://tede.ufam.edu.br/handle/tede/8028.

Council of Science Editors:

Costa FC. Segmenta??o sem?ntica de ?reas desmatadas utilizando Redes Neurais Convolucionais no sul da Amaz?nia Legal. [Masters Thesis]. Universidade Federal do Amazonas; 2020. Available from: https://tede.ufam.edu.br/handle/tede/8028

Ruhr Universität Bochum

26. Goh, Jinzhong Jeremy. Characterization of the effects of novel object-space information on synaptic plasticity in the hippocampal CA1 sub- region of freely behaving mice.

Degree: 2012, Ruhr Universität Bochum

URL: http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-36198

► Der Hippocampus spielt eine wichtige Rolle bei der Bildung von Gedächtnis. Die vorliegende Studie dient zur Klärung der Frage nach der synaptischen Änderung, die sich… (more)

Subjects/Keywords: Elektrophysiologie; Maus; In vivo; Neuronale Plastizität; Hippocampus

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Goh, J. J. (2012). Characterization of the effects of novel object-space information on synaptic plasticity in the hippocampal CA1 sub- region of freely behaving mice. (Thesis). Ruhr Universität Bochum. Retrieved from http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-36198

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Goh, Jinzhong Jeremy. “Characterization of the effects of novel object-space information on synaptic plasticity in the hippocampal CA1 sub- region of freely behaving mice.” 2012. Thesis, Ruhr Universität Bochum. Accessed March 05, 2021. http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-36198.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Goh, Jinzhong Jeremy. “Characterization of the effects of novel object-space information on synaptic plasticity in the hippocampal CA1 sub- region of freely behaving mice.” 2012. Web. 05 Mar 2021.

Vancouver:

Goh JJ. Characterization of the effects of novel object-space information on synaptic plasticity in the hippocampal CA1 sub- region of freely behaving mice. [Internet] [Thesis]. Ruhr Universität Bochum; 2012. [cited 2021 Mar 05]. Available from: http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-36198.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Goh JJ. Characterization of the effects of novel object-space information on synaptic plasticity in the hippocampal CA1 sub- region of freely behaving mice. [Thesis]. Ruhr Universität Bochum; 2012. Available from: http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-36198

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Ruhr Universität Bochum

27. Ziebarth, Wibke. Der Einfluss von 5-Aminosalicylsäure auf die Progression kolorektaler Adenome in vivo.

Degree: 2010, Ruhr Universität Bochum

URL: http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-28823

► Das kolorektale Adenom gilt als Vorläuferläsion des kolorektalen Karzinoms, wobei die Dysregulation des APC/β- catenin-Signalweges in der Adenom-Karzinom-Sequenz ein frühes Schlüsselereignis ist. Ziel dieser Arbeit… (more)

Subjects/Keywords: Dickdarmkrebs; Adenom; In vivo; Mesalazin; Adenom / Rückbildung

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ziebarth, W. (2010). Der Einfluss von 5-Aminosalicylsäure auf die Progression kolorektaler Adenome in vivo. (Thesis). Ruhr Universität Bochum. Retrieved from http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-28823

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ziebarth, Wibke. “Der Einfluss von 5-Aminosalicylsäure auf die Progression kolorektaler Adenome in vivo.” 2010. Thesis, Ruhr Universität Bochum. Accessed March 05, 2021. http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-28823.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ziebarth, Wibke. “Der Einfluss von 5-Aminosalicylsäure auf die Progression kolorektaler Adenome in vivo.” 2010. Web. 05 Mar 2021.

Vancouver:

Ziebarth W. Der Einfluss von 5-Aminosalicylsäure auf die Progression kolorektaler Adenome in vivo. [Internet] [Thesis]. Ruhr Universität Bochum; 2010. [cited 2021 Mar 05]. Available from: http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-28823.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ziebarth W. Der Einfluss von 5-Aminosalicylsäure auf die Progression kolorektaler Adenome in vivo. [Thesis]. Ruhr Universität Bochum; 2010. Available from: http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-28823

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Ruhr Universität Bochum

28. Buschler, Arne. Characterisation of hippocampal synaptic plasticity in freely behaving mice and its modulation by environmental enrichment.

Degree: 2012, Ruhr Universität Bochum

URL: http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-37433

► Synaptische Plastizität gilt als Mechanismus, der Lern- und Gedächtnisvorgängen zugrunde liegt. Dabei spielt auf molekularer Ebene der NMDA-Rezeptor, besonders an den Schaffer-Kollateralen-CA1-Synapsen, eine große Rolle.… (more)

Subjects/Keywords: Neuronale Plastizität; In vivo; Elektrophysiologie; Langzeitpotenzierung; Laus

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Buschler, A. (2012). Characterisation of hippocampal synaptic plasticity in freely behaving mice and its modulation by environmental enrichment. (Thesis). Ruhr Universität Bochum. Retrieved from http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-37433

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Buschler, Arne. “Characterisation of hippocampal synaptic plasticity in freely behaving mice and its modulation by environmental enrichment.” 2012. Thesis, Ruhr Universität Bochum. Accessed March 05, 2021. http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-37433.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Buschler, Arne. “Characterisation of hippocampal synaptic plasticity in freely behaving mice and its modulation by environmental enrichment.” 2012. Web. 05 Mar 2021.

Vancouver:

Buschler A. Characterisation of hippocampal synaptic plasticity in freely behaving mice and its modulation by environmental enrichment. [Internet] [Thesis]. Ruhr Universität Bochum; 2012. [cited 2021 Mar 05]. Available from: http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-37433.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Buschler A. Characterisation of hippocampal synaptic plasticity in freely behaving mice and its modulation by environmental enrichment. [Thesis]. Ruhr Universität Bochum; 2012. Available from: http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-37433

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Ruhr Universität Bochum

29. Merkendorf, Tobias. Biophysikalische Untersuchungen am Protonenpumpmechanismus von Rhodopsinen aus Halobacterium salinarum und Haloarcula marismortui.

Degree: 2014, Ruhr Universität Bochum

URL: http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-41178

► Mikrobielle Rhodopsine sind eine weitverbreitete Gruppe von Membranproteinen mit vielen unterschiedlichen Funktionen. Eine davon ist, lichtgetrieben Protonen über eine Membran zu transportieren und einen Protonengradienten… (more)

Subjects/Keywords: Bakteriorhodopsin; Membrantransport; Photozyklus; In vivo; Optogenetik

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Merkendorf, T. (2014). Biophysikalische Untersuchungen am Protonenpumpmechanismus von Rhodopsinen aus Halobacterium salinarum und Haloarcula marismortui. (Thesis). Ruhr Universität Bochum. Retrieved from http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-41178

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Merkendorf, Tobias. “Biophysikalische Untersuchungen am Protonenpumpmechanismus von Rhodopsinen aus Halobacterium salinarum und Haloarcula marismortui.” 2014. Thesis, Ruhr Universität Bochum. Accessed March 05, 2021. http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-41178.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Merkendorf, Tobias. “Biophysikalische Untersuchungen am Protonenpumpmechanismus von Rhodopsinen aus Halobacterium salinarum und Haloarcula marismortui.” 2014. Web. 05 Mar 2021.

Vancouver:

Merkendorf T. Biophysikalische Untersuchungen am Protonenpumpmechanismus von Rhodopsinen aus Halobacterium salinarum und Haloarcula marismortui. [Internet] [Thesis]. Ruhr Universität Bochum; 2014. [cited 2021 Mar 05]. Available from: http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-41178.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Merkendorf T. Biophysikalische Untersuchungen am Protonenpumpmechanismus von Rhodopsinen aus Halobacterium salinarum und Haloarcula marismortui. [Thesis]. Ruhr Universität Bochum; 2014. Available from: http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-41178

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

30. Cristina Apolinário da Silva, Andréa. Novas moléculas imidazolidínicas: potenciais candidatas a fármacos esquistossomicidas .

Degree: 2008, Universidade Federal de Pernambuco

URL: http://repositorio.ufpe.br/handle/123456789/2183

► A esquistossomose é uma doença parasitária crônica das regiões tropicais e subtropicais e está associada a uma alta morbidade da população infectada. A terapia da… (more)

▼ A esquistossomose é uma doença parasitária crônica das regiões tropicais e subtropicais e está associada a uma alta morbidade da população infectada. A terapia da esquistossomose é feita apenas com um único fármaco, o praziquantel, ativo contra todas as espécies de Schistosoma, e o oxamniquina ativo apenas contra o Schistosoma mansoni. Contudo há relatos na literatura de resistência do parasito ao tratamento para os dois fármacos, gerando uma preocupação a nível mundial e levando a Organização Mundial de Saúde (OMS) convocar a indústria e os pesquisadores a buscar novos fármacos com esta finalidade. Neste contexto a busca de novos fármacos com atividade esquistossomicida torna-se o objetivo deste trabalho, onde os derivados imidazolidínicos das séries 5-benzilideno-3-benzil-4-tioxo-imidazolidin-2-ona (LPSF/PT), 5-benzilideno-3-(4-metil-benzil)-imidazolidina-2,4-diona (LPSF/MS) e 5-arilazo-4-tioxo-imidazolidin-2-ona (LPSF/PT) foram avaliados in vitro frente a vermes adultos de Schistosoma mansoni (Cepa BH). Dos derivados avaliados in vitro os que apresentaram atividade esquistossomicida, foram os derivados da série 3-benzil-5-(arilazo)-4-tioxo-imidazolidin-2-ona (LPSF/PT). Para a avaliação in vivo, o derivado 3-benzil-5-(4-cloro-arilazo)-4-tioxoimidazolidin- 2-ona (LPSF/PT05) foi administrado oralmente em Tween 80, Tween/óleo/água e em dispersão sólida com 90 % PEG (Polietilenoglicol). Apenas esta última apresentou efeito esquistossomicida. Foram administradas quatro doses: 3, 10, 30 e 100 mg/kg. A redução do número de vermes recuperados foi significativa para a dose de 10 e 30 mg/kg, aproximadamente 50 % e para a dose de 100 mg/kg a redução foi de 68%. A redução do número de vermes na dose de 100 mg/kg corroborou com a resposta imune celular do derivado e levantou a hipótese do seu efeito imunossupressor ser ao nível de resposta Th1 e Th2. Somado a esses dados a resposta histopatológica revelou uma modulação na resposta granulomatosa, produziu uma ação contra o dano hepático causado pela infecção pelo S. mansoni Advisors/Committee Members: Lins Galdino, Suely (advisor).

Subjects/Keywords: Esquistossomose; Atividade Esquistossomicida; Imidazolidina; Avaliação in vivo

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Cristina Apolinário da Silva, A. (2008). Novas moléculas imidazolidínicas: potenciais candidatas a fármacos esquistossomicidas . (Thesis). Universidade Federal de Pernambuco. Retrieved from http://repositorio.ufpe.br/handle/123456789/2183

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Cristina Apolinário da Silva, Andréa. “Novas moléculas imidazolidínicas: potenciais candidatas a fármacos esquistossomicidas .” 2008. Thesis, Universidade Federal de Pernambuco. Accessed March 05, 2021. http://repositorio.ufpe.br/handle/123456789/2183.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Cristina Apolinário da Silva, Andréa. “Novas moléculas imidazolidínicas: potenciais candidatas a fármacos esquistossomicidas .” 2008. Web. 05 Mar 2021.

Vancouver:

Cristina Apolinário da Silva A. Novas moléculas imidazolidínicas: potenciais candidatas a fármacos esquistossomicidas . [Internet] [Thesis]. Universidade Federal de Pernambuco; 2008. [cited 2021 Mar 05]. Available from: http://repositorio.ufpe.br/handle/123456789/2183.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Cristina Apolinário da Silva A. Novas moléculas imidazolidínicas: potenciais candidatas a fármacos esquistossomicidas . [Thesis]. Universidade Federal de Pernambuco; 2008. Available from: http://repositorio.ufpe.br/handle/123456789/2183

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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Open Access Theses and Dissertations