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You searched for subject:(SufR). Showing records 1 – 2 of 2 total matches.

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Penn State University

1. Wu, Yingxian. REGULATION OF IRON-SULFUR CLUSTER BIOGENESIS IN CYANOBACTERIA .

Degree: 2008, Penn State University

In this thesis, I present my studies on the two regulators for iron-sulfur cluster biogenesis in cyanobacteria, SYNPCC7002_A0128 and SufR. SYNPCC7002_A0128 is a homologue of IscR, the transcriptional regulator for iron-sulfur cluster biogenesis in Escherichia coli. To investigate the role of SYNPCC7002_A0128, a null-mutant strain of SYNPCC7002_A0128 was constructed in Synechococcus sp. PCC 7002. Physiological features were characterized and RT-PCR analyses were conducted for the mutant. The physiological features and the changes in the transcription profiles for the isc genes and the suf genes at the deletion of SYNPCC7002_A0128 revealed a regulatory role for SYNPCC7002_A0128 in iron-sulfur cluster biogenesis. SufR is found to be an iron-sulfur cluster containing, transcriptional regulator for the suf operon in cyanobacterium Synechocystis sp. PCC 6803. I continued the process of characterizing the [4Fe-4S]2+,1+ cluster in the SufR holo protein. Chemical analysis and spectroscopic analyses showed that SufR coordinated two [4Fe-4S]2+,1+ clusters per dimer. EPR spectroscopy revealed that reduced [4Fe-4S]2+,1+ cluster in SufR existed in a mixture of S = 1/2 and S = 3/2 ground spin states, with the S = 1/2 state accounting for 44% of the total spins. These results, combined with previous studies on the cysteine variants, suggested that there is one non-cysteine ligand that binds each [4Fe-4S]2+,1+ cluster in SufR Advisors/Committee Members: Sprio Stefanou, Thesis Advisor/Co-Advisor, John H Golbeck, Thesis Advisor/Co-Advisor, Donald Ashley Bryant, Thesis Advisor/Co-Advisor.

Subjects/Keywords: cyanobacteria; Iron-sulfur cluster; SufR

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Wu, Y. (2008). REGULATION OF IRON-SULFUR CLUSTER BIOGENESIS IN CYANOBACTERIA . (Thesis). Penn State University. Retrieved from https://submit-etda.libraries.psu.edu/catalog/8592

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Wu, Yingxian. “REGULATION OF IRON-SULFUR CLUSTER BIOGENESIS IN CYANOBACTERIA .” 2008. Thesis, Penn State University. Accessed November 26, 2020. https://submit-etda.libraries.psu.edu/catalog/8592.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Wu, Yingxian. “REGULATION OF IRON-SULFUR CLUSTER BIOGENESIS IN CYANOBACTERIA .” 2008. Web. 26 Nov 2020.

Vancouver:

Wu Y. REGULATION OF IRON-SULFUR CLUSTER BIOGENESIS IN CYANOBACTERIA . [Internet] [Thesis]. Penn State University; 2008. [cited 2020 Nov 26]. Available from: https://submit-etda.libraries.psu.edu/catalog/8592.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Wu Y. REGULATION OF IRON-SULFUR CLUSTER BIOGENESIS IN CYANOBACTERIA . [Thesis]. Penn State University; 2008. Available from: https://submit-etda.libraries.psu.edu/catalog/8592

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation


Penn State University

2. Wang, Tao. iron sulfur cluster assembly in photosystem I of cyanobacteria: the role of SufR (SUF) and Fdx (ISC).

Degree: 2008, Penn State University

The assembly/biogenesis of Photosystem I is poorly understood compared with its well-documented structure and function studies. As a multi-subunit membrane pigment-protein complex, many factors affect its assembly. Among them three [4Fe-4S] clusters are functionally and structurally essential. Any single cysteine ligand change would result in reduction or complete loss of PS I complex in cyanobacteria. Fe-S cluster assembly is a complicated bioprocess involving several protein factors. So far three Fe-S cluster assembly pathways have been identified, namely NIF, ISC, and SUF. Cyanobacteria are distinguished from other organisms in regard to Fe-S assembly, as it contains four nifS-like homologs representing three possible pathways. slr0387 and sll0704 are iscS-like homologs (ISC), slr0077 is sufS-like homolog (SUF), and slr2143 does not belong to any known Fe-S cluster assembly pathway so far. To investigate which one is involved in Fe-S cluster assembly in PS I, two strategies were applied to start study on the regulation of Fe-S cluster assembly into PS I. 1), pseudorevertant screening of C14SPsaC mutant in Synechocystis sp. PCC 6803 to find regulators of Fe-S cluster assembly; 2), molecular and genetic manipulation on homologous genes encoding components of known Fe-S cluster assembly pathway. For the first strategy, sll0088 was found to be involved in the assembly of C14SPsaC mutant protein into PS I complex by pseudorevertant screening. Amino acid sequence alignment of Sll0088 homologs indicated that Sll0088 contains an HTH DNA binding domain and a unique cysteine motif CX12CX13CX14C. In almost all cyanobacterial genomes sequenced so far, sll0088-like gene is located immediately upstream of a suf gene cluster and divergently transcribed. To investigate if it encodes a transcription regulator, the sll0088-like gene in Synechococcus sp. PCC 7002 was insertionally inactivated. RT-PCR analysis showed that the four suf genes organize as an operon and their transcription was up-regulated in sll0088-like inactivation mutant. sll0088-like was thus renamed sufR (suf repressor). SufR also has other regulons, such as slr0387 (iscS homolog), which is down-regulated in sufR inactivation mutant. SufR protein was overexpressed in E. coli and purified. EPR and Mössbauer studies indicated that reconstituted SufR is a [4Fe-4S] protein. The ratio is one Fe-S cluster per dimer. Amino acid substitution study suggested that the 1st, 2nd and 4th cysteine residues in the cysteine motif provide ligands to Fe-S cluster. Replacement of the 3rd one with either serine or glycine resulted in neglectable EPR signal changes and thus this cysteine may not participate in Fe-S cluster ligation. The reconstituted Fe-S cluster is oxygen sensitive. Physiologically, SufR protein may use [4Fe-4S] cluster as a sensor to respond to oxidative stress and iron deficiency, as under both conditions the suf genes were derepressed. The SufR protein was also found to be distributed into two compartments by immunoblot analysis, the majority exists in the cytosol,… Advisors/Committee Members: John H Golbeck, Committee Chair/Co-Chair, Donald Ashley Bryant, Committee Chair/Co-Chair, Teh Hui Kao, Committee Member, Hong Ma, Committee Member.

Subjects/Keywords: Iron-sulfur cluster; assembly; biogenesis; PS I; SufR; Fdx; cyanobacteria

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Wang, T. (2008). iron sulfur cluster assembly in photosystem I of cyanobacteria: the role of SufR (SUF) and Fdx (ISC). (Thesis). Penn State University. Retrieved from https://submit-etda.libraries.psu.edu/catalog/6216

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Wang, Tao. “iron sulfur cluster assembly in photosystem I of cyanobacteria: the role of SufR (SUF) and Fdx (ISC).” 2008. Thesis, Penn State University. Accessed November 26, 2020. https://submit-etda.libraries.psu.edu/catalog/6216.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Wang, Tao. “iron sulfur cluster assembly in photosystem I of cyanobacteria: the role of SufR (SUF) and Fdx (ISC).” 2008. Web. 26 Nov 2020.

Vancouver:

Wang T. iron sulfur cluster assembly in photosystem I of cyanobacteria: the role of SufR (SUF) and Fdx (ISC). [Internet] [Thesis]. Penn State University; 2008. [cited 2020 Nov 26]. Available from: https://submit-etda.libraries.psu.edu/catalog/6216.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Wang T. iron sulfur cluster assembly in photosystem I of cyanobacteria: the role of SufR (SUF) and Fdx (ISC). [Thesis]. Penn State University; 2008. Available from: https://submit-etda.libraries.psu.edu/catalog/6216

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

.