subject:(Streptococcus agalactiae)

Universidad de Chile

1. Hernández Astudillo, Carlos Felipe. Caracterización de la respuesta inmune celular inducida por un prototipo de vacuna oral contra Streptococcus agalactiae en modelo murino.

Degree: 2020, Universidad de Chile

URL: http://repositorio.uchile.cl/handle/2250/176029

► Streptococcus agalactiae (Streptococco del grupo B. SGB) es un patógeno oportunista capaz de colonizar el tracto genital y gastrointestinal de aproximadamente un 50% de los… (more)

▼ Streptococcus agalactiae (Streptococco del grupo B. SGB) es un patógeno oportunista capaz de colonizar el tracto genital y gastrointestinal de aproximadamente un 50% de los adultos sanos. SGB está asociada a parto prematuro y muerte fetal y es el principal agente etiológico de enfermedades neonatales como neumonía, septicemia y meningitis. No existen vacunas licenciadas a la fecha y se encuentran dos vacunas en estudio de fase clínica. Los antígenos de Proteínas estructuralmente conservadas de SGB son buenos inmunógenos y son considerados como atractivos blancos para el desarrollo de vacunas. Es este sentido, la proteína inmunogénica de superficie (SIP), es considerada un blanco para el desarrollo de una vacuna, debido a su alta capacidad inmunogénica y a que es altamente conservada en todos los serotipos de SGB. Se ha descrito que la inmunización subcutánea de SIP recombinante (rSIP) sin adyuvantes, induce altos niveles de anticuerpos y disminuye la colonización intravaginal. Adicionalmente cuando se inmuniza con rSIP más Alum como adyuvante de forma oral se observa una respuesta inmune protectora del tipo Th1, dada por el perfil de citoquinas, y subclases de Ig. Por otro lado, se evaluó la vacunación intraperitoneal de rSIP más AbISCO-100 como adyuvante, donde se observa que la transferencia de células CD4 y CD8 en proporción 1:1, de ratones vacunados hacia ratones naive, genera protección frente a la infección intravaginal de SGB. Esta es la primera observación de respuesta celular de SGB, la cual por si sola es capaz de generar protección contra SGB. Además, nuestro laboratorio desarrollo un prototipo de vacuna oral basada en L. lactis que secreta la proteína SIP, la cual estimula una respuesta inmune caracterizada por la secreción de anticuerpos del tipo IgG e IgA, una disminución de la colonización vaginal de SGB y activación de Linfocitos T. En base a estos antecedentes se propone que L. lactis secretora de SIP genera una respuesta inmune celular capaz de generar protección contra SGB. Considerando esto, se plantea la hipótesis que la respuesta celular estimulada por la vacuna oral en base a L. lactis que secreta SIP estimula a una protección contra la colonización intravaginal de SGB en modelo murino. Para lo cual, se caracterizó la respuesta inmune celular mediante marcadores de activación de linfocitos y se observó que se induce activación de linfocitos T CD4 y CD8 dada por el marcador de activación CD69. La expresión de los factores de transcripción FOXP3 demostró que la vacuna no genera tolerancia dado que no se estimuló la activación de linfocitos T reguladores (CD4+CD25+FOXP3+) Por último, se determinó que esta respuesta celular dada por la activación de linfocitos T CD4 y CD8 confiere protección contra SGB dado que estas células purificadas desde ratones estimulados con la vacuna son capaces de disminuir la colonización intravaginal de SGB en ratones que nunca han visto el antígeno, demostrando que no solamente la vacuna genera activación de células T, sino también…

Subjects/Keywords: Streptococcus agalactiae

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hernández Astudillo, C. F. (2020). Caracterización de la respuesta inmune celular inducida por un prototipo de vacuna oral contra Streptococcus agalactiae en modelo murino. (Thesis). Universidad de Chile. Retrieved from http://repositorio.uchile.cl/handle/2250/176029

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Hernández Astudillo, Carlos Felipe. “Caracterización de la respuesta inmune celular inducida por un prototipo de vacuna oral contra Streptococcus agalactiae en modelo murino.” 2020. Thesis, Universidad de Chile. Accessed January 16, 2021. http://repositorio.uchile.cl/handle/2250/176029.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Hernández Astudillo, Carlos Felipe. “Caracterización de la respuesta inmune celular inducida por un prototipo de vacuna oral contra Streptococcus agalactiae en modelo murino.” 2020. Web. 16 Jan 2021.

Vancouver:

Hernández Astudillo CF. Caracterización de la respuesta inmune celular inducida por un prototipo de vacuna oral contra Streptococcus agalactiae en modelo murino. [Internet] [Thesis]. Universidad de Chile; 2020. [cited 2021 Jan 16]. Available from: http://repositorio.uchile.cl/handle/2250/176029.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hernández Astudillo CF. Caracterización de la respuesta inmune celular inducida por un prototipo de vacuna oral contra Streptococcus agalactiae en modelo murino. [Thesis]. Universidad de Chile; 2020. Available from: http://repositorio.uchile.cl/handle/2250/176029

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidad Andrés Bello

2. Traipe Alvarez, Darinka Nicole. Estudio de portación de Streptococcusagalactiae, serotipificación y detección de fenotipos de resistencia MLSb, M y L, en una población femenina sana en edad fértil de estudiantes de Tecnología Médica, sede República de la Universidad Andrés Bello .

Degree: 2015, Universidad Andrés Bello

URL: http://repositorio.unab.cl/xmlui/handle/ria/2654

► Streptococcusagalactiae es una bacteria perteneciente al género Streptococcus β- hemolíticos. Presenta un polisacárido común que la clasifica como Streptococcus grupo B (SGB). Además se ha… (more)

▼ Streptococcusagalactiae es una bacteria perteneciente al género Streptococcus β- hemolíticos. Presenta un polisacárido común que la clasifica como Streptococcus grupo B (SGB). Además se ha subdividido en diez serotipos (Ia, Ib, II a IX) basados en los antígenos de polisacáridos capsulares y antígenos proteicos. (1) SGB forma parte de la microbiota intestinal, desde donde puede colonizar la vagina, el tracto genitourinario y el tracto respiratorio. (2) La colonización del tracto genital puede ser intermitente y es un hecho significativo en mujeres embarazadas, por la posibilidad de transmisión de este microorganismo al recién nacido provocando la enfermedad neonatal de comienzo precoz y la enfermedad neonatal de comienzo tardío. Este hecho es un importante problema de salud pública a nivel mundial y nacional, ya que SGB es el principal agente causal de neumonía, meningitis, bacteriemia y sepsis neonatal. (3) Además también puede afectar a mujeres gestantes y pacientes con compromiso inmunológico. (4). El objetivo de la presente unidad de investigación busca detectar el porcentaje de colonización de Streptococcusagalactiae en una población femenina sana en edad fértil de estudiantes de Tecnología Médica de la Universidad Andrés Bello (sede República). Además de identificar los serotipos más frecuentes de este microorganismo, a través el Kit SerotipdeS.agalactiae grupo B (Denka- Seiken) y se determinaran losfenotipos de resistencia MLSb (Macrólidos, Lincosamidas, Estreptograminas del grupo b), M (Macrólidos) y L (Lincosamidas), cuantificando su frecuencia 7 respectiva. Adicionalmente se evaluará la sensibilidad a penicilina a través de la técnica Kirby – Bauer (difusión en agar). Se realizó un cultivo vaginal a 234 mujeres estudiantes de Tecnología Médica de la Universidad Andrés Bello entre las edades de 18 a 31 años, de las cuales 32 resultaron ser portadoras de SGB (13,6%). Se identificó al serotipo III como el más frecuente, seguido del serotipo Ia. Con respecto a la susceptibilidad antimicrobiana, de las 32 cepas de SGB, 19 cepas resultaron ser sensibles a eritromicina y clindamicina, mientras que las 13 cepas restantes presentaron resistencia a eritromicina y clindamicina con un fenotipo de resistencia iMLSb, siendo el 100% de estas cepas pertenecientes al serotipo III. Conocer el porcentaje de portación de SGB en mujeres no embarazadas en Chile, junto con la caracterización fenotípica y los serotipos más frecuentes, aporta importantes datos epidemiológicos, permitiendo conocer la distribución y frecuencia de los serotipos, para la fabricación de futuras vacunas contra este microorganismo y así disminuir el abuso de la profilaxis antibiótica intraparto, lo cual a través del tiempo ha generado resistencia en otros microorganismos. Advisors/Committee Members: Rojo Lira, Patricia Isabel (advisor).

Subjects/Keywords: Streptococcus Agalactiae; Mujeres

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Traipe Alvarez, D. N. (2015). Estudio de portación de Streptococcusagalactiae, serotipificación y detección de fenotipos de resistencia MLSb, M y L, en una población femenina sana en edad fértil de estudiantes de Tecnología Médica, sede República de la Universidad Andrés Bello . (Thesis). Universidad Andrés Bello. Retrieved from http://repositorio.unab.cl/xmlui/handle/ria/2654

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Traipe Alvarez, Darinka Nicole. “Estudio de portación de Streptococcusagalactiae, serotipificación y detección de fenotipos de resistencia MLSb, M y L, en una población femenina sana en edad fértil de estudiantes de Tecnología Médica, sede República de la Universidad Andrés Bello .” 2015. Thesis, Universidad Andrés Bello. Accessed January 16, 2021. http://repositorio.unab.cl/xmlui/handle/ria/2654.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Traipe Alvarez, Darinka Nicole. “Estudio de portación de Streptococcusagalactiae, serotipificación y detección de fenotipos de resistencia MLSb, M y L, en una población femenina sana en edad fértil de estudiantes de Tecnología Médica, sede República de la Universidad Andrés Bello .” 2015. Web. 16 Jan 2021.

Vancouver:

Traipe Alvarez DN. Estudio de portación de Streptococcusagalactiae, serotipificación y detección de fenotipos de resistencia MLSb, M y L, en una población femenina sana en edad fértil de estudiantes de Tecnología Médica, sede República de la Universidad Andrés Bello . [Internet] [Thesis]. Universidad Andrés Bello; 2015. [cited 2021 Jan 16]. Available from: http://repositorio.unab.cl/xmlui/handle/ria/2654.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Traipe Alvarez DN. Estudio de portación de Streptococcusagalactiae, serotipificación y detección de fenotipos de resistencia MLSb, M y L, en una población femenina sana en edad fértil de estudiantes de Tecnología Médica, sede República de la Universidad Andrés Bello . [Thesis]. Universidad Andrés Bello; 2015. Available from: http://repositorio.unab.cl/xmlui/handle/ria/2654

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidad de Chile

3. Leyton Galaz, Yessica. Evaluación de respuesta inmune protectora de vacuna oral de subunidad de la proteína inmunológica de superficie de Streptococcus agalactiae en modelo murino.

Degree: 2016, Universidad de Chile

URL: http://repositorio.uchile.cl/handle/2250/171110

► Streptococcus agalactiae (SGB) es una bacteria Gram positiva anaeróbica facultativa capaz de colonizar el tracto genitourinario de un 15 a un 40% de mujeres sexualmente… (more)

▼ Streptococcus agalactiae (SGB) es una bacteria Gram positiva anaeróbica facultativa capaz de colonizar el tracto genitourinario de un 15 a un 40% de mujeres sexualmente activas. En la mayoría de los casos la infección con este patógeno es asintomática pero en neonatos puede originar sepsis, meningitis y neumonía. A partir del año 1990 el CDC (Center for Disease Control and Prevention) ha implementado una serie de protocolos de prevención dirigidos a mujeres embarazadas los cuales constan de pruebas microbiológicas realizadas entre las 35 y 37 semanas de gestación y la aplicación de antibióticos intraparto en mujeres colonizadas. A pesar de que medidas han tenido un alto impacto en la disminución de la incidencia de la infección por SGB este patógeno persiste como el único y más frecuente aislado desde la sangre o del fluido cerebroespinal de infantes menores de 3 meses de edad y de mujeres con infecciones intraparto en Estados Unidos y en otros países industrializados. Numerosos estudios realizados a neonatos sanos nacidos de madres colonizadas con SGB indican que estos tienen niveles superiores de anticuerpos IgG transferidos transplacentalmente que aquellos que desarrollaron la infección. Basado en este hecho actualmente se trabaja arduamente en el desarrollo de una vacuna capaz proteger a mujeres embarazadas y sus infantes. En la actualidad, vacunas en base a polisacáridos conjugados están en Fase clínica III, con el inconveniente de que la vacuna ensayada será serotipo dependiente y solamente inducirá una respuesta inmune protectora contra los serotipos incluidos en su formulación (3 de 10 serotipos). Vacunas en base a proteínas conservadas de SGB se encuentran en estudio, una de ellas es la proteína SIP (Surface Immunogenic Protein), con la cual se trabaja en la Sección de Biotecnología del ISPCH. En este estudio se evaluará la respuesta inmune protectora de un prototipo de vacuna en base a la proteína recombinante SIP de SGB, evaluando parámetros inmunológicos que permitan demostrar la inducción de inmunidad protectora en modelo ratón

Subjects/Keywords: Streptococcus agalactiae; Vacunas

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Leyton Galaz, Y. (2016). Evaluación de respuesta inmune protectora de vacuna oral de subunidad de la proteína inmunológica de superficie de Streptococcus agalactiae en modelo murino. (Thesis). Universidad de Chile. Retrieved from http://repositorio.uchile.cl/handle/2250/171110

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Leyton Galaz, Yessica. “Evaluación de respuesta inmune protectora de vacuna oral de subunidad de la proteína inmunológica de superficie de Streptococcus agalactiae en modelo murino.” 2016. Thesis, Universidad de Chile. Accessed January 16, 2021. http://repositorio.uchile.cl/handle/2250/171110.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Leyton Galaz, Yessica. “Evaluación de respuesta inmune protectora de vacuna oral de subunidad de la proteína inmunológica de superficie de Streptococcus agalactiae en modelo murino.” 2016. Web. 16 Jan 2021.

Vancouver:

Leyton Galaz Y. Evaluación de respuesta inmune protectora de vacuna oral de subunidad de la proteína inmunológica de superficie de Streptococcus agalactiae en modelo murino. [Internet] [Thesis]. Universidad de Chile; 2016. [cited 2021 Jan 16]. Available from: http://repositorio.uchile.cl/handle/2250/171110.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Leyton Galaz Y. Evaluación de respuesta inmune protectora de vacuna oral de subunidad de la proteína inmunológica de superficie de Streptococcus agalactiae en modelo murino. [Thesis]. Universidad de Chile; 2016. Available from: http://repositorio.uchile.cl/handle/2250/171110

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidad de Chile

4. Manzo Paredes, Ricardo Augusto. Proteína inmunogénica de superficie de streptococcus agalactiae : estudio del mecanismo de su capacidad adyuvante inmunológico.

Degree: 2019, Universidad de Chile

URL: http://repositorio.uchile.cl/handle/2250/175136

► Streptococcus agalactiae también conocida como Streptococcus Grupo B (SGB), es una cocaccea Gram positiva, anaeróbica, facultativa y β-hemolítica capaz de colonizar el tracto genitourinario de… (more)

▼ Streptococcus agalactiae también conocida como Streptococcus Grupo B (SGB), es una cocaccea Gram positiva, anaeróbica, facultativa y β-hemolítica capaz de colonizar el tracto genitourinario de un 15% a un 40% de mujeres embarazadas. Se asocia a la muerte fetal y parto prematuro. Así como es la principal causa de sepsis neonatal. El desarrollo de una vacuna contra SGB es prioritario según la OMS, hay vacunas de polisacáridos conjugadas que están en fase clínica II y existen otras vacunas en base a proteínas purificadas que se encuentran en fase clínica I y a nivel preclínico. Una de las proteínas que ha demostrado ser atractiva desde el punto de vista inmunológico como una alternativa para el desarrollo de una vacuna contra SGB, es la proteína inmunogénica de superficie conocida por sus siglas en inglés de SIP (surface immunogenic protein).En los 10 serotipos descritos a la fecha para SGB , esta proteína es altamente conservada en su secuencia aminoacídica y se ha ensayado como potencial inmunógeno para el desarrollo de una vacuna. La función biológica de esta proteína aún no ha sido descrita. En la Sección de Biotecnología del Instituto de Salud Pública de Chile se obtuvo la proteína SIP recombinante desde una cepa de SGB proveniente de un cuadro invasivo de sepsis y meningitis. Esta proteína se ha evaluado como prototipo de vacuna a nivel preclínico mediante inmunización oral y subcutánea, mostrándose que al ser inmunizada por la vía oral es capaz de conferir inmunidad protectora en un modelo murino. Además, esta proteína al ser inmunizada por la vía subcutánea sin la presencia de adyuvantes, confiere inmunidad protectora, que permite disminuir la colonización intravaginal por SGB, lo que nos hace suponer que esta proteína podría tener propiedades de adyuvante inmunológico para el desarrollo de vacunas. En esta tesis se obtuvo la proteína recombinante SIP desde E. coli BL21 (DE3) Codon plus mediante cromatografía de afinidad baja presión y su pureza se confirmó mediante cromatografía de exclusión molecular en HPLC. Se evaluó su capacidad de potenciar la respuesta inmune contra ova-albúmina (OVA) al inmunizar ratones de la cepa C57BL/6 con una mezcla de proteínas. La proteína rSIP formulada como adyuvante indujo mayor cantidad de anticuerpos anti-OVA que la formulación con OVA sola y con OVA más adyuvante ALUM. El estudio realizado en células dendríticas (DCs) permitió observar que rSIP indujo un cambio fenotípico en dichas células aumentando el marcador de superficie CD86/80 y DC 40 y MHCII. Respecto a los parámetros inmunológicos se observó que el sobrenadante de las DCs estimuladas con rSIP indujo un aumento de las citoquinas IL-10 y IL-12. La evaluación de la proteína rSIP como ligando de TLR muestra que es un agonista de TLR2 y TL4. Estudios preliminares de la estructura de la proteína rSIP permiten concluir que la estructura secundaria es mayoritariamente beta en su estado plegado, mientras que con 3,9 M de GdHCl está principalmente desplegada. Los resultados…

Subjects/Keywords: Streptococcus agalactiae; Proteínas recombinantes

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Manzo Paredes, R. A. (2019). Proteína inmunogénica de superficie de streptococcus agalactiae : estudio del mecanismo de su capacidad adyuvante inmunológico. (Thesis). Universidad de Chile. Retrieved from http://repositorio.uchile.cl/handle/2250/175136

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Manzo Paredes, Ricardo Augusto. “Proteína inmunogénica de superficie de streptococcus agalactiae : estudio del mecanismo de su capacidad adyuvante inmunológico.” 2019. Thesis, Universidad de Chile. Accessed January 16, 2021. http://repositorio.uchile.cl/handle/2250/175136.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Manzo Paredes, Ricardo Augusto. “Proteína inmunogénica de superficie de streptococcus agalactiae : estudio del mecanismo de su capacidad adyuvante inmunológico.” 2019. Web. 16 Jan 2021.

Vancouver:

Manzo Paredes RA. Proteína inmunogénica de superficie de streptococcus agalactiae : estudio del mecanismo de su capacidad adyuvante inmunológico. [Internet] [Thesis]. Universidad de Chile; 2019. [cited 2021 Jan 16]. Available from: http://repositorio.uchile.cl/handle/2250/175136.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Manzo Paredes RA. Proteína inmunogénica de superficie de streptococcus agalactiae : estudio del mecanismo de su capacidad adyuvante inmunológico. [Thesis]. Universidad de Chile; 2019. Available from: http://repositorio.uchile.cl/handle/2250/175136

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

5. Cavallaro, Antonino Giovanni. Streptococcus agalactiae e colonizzazione materna: valutazione dei meccanismi di virulenza e chemioprofilassi.

Degree: 2011, Università degli Studi di Catania

URL: http://hdl.handle.net/10761/165

► Negli ultimi anni lo Streptococco di gruppo B (GBS) ha assunto un ruolo prioritario nel determinismo della sepsi neonatale, con un tasso di mortalita' del… (more)

Subjects/Keywords: Streptococcus agalactiae; Infezione materna; Chemioprofilassi

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Cavallaro, A. G. (2011). Streptococcus agalactiae e colonizzazione materna: valutazione dei meccanismi di virulenza e chemioprofilassi. (Thesis). Università degli Studi di Catania. Retrieved from http://hdl.handle.net/10761/165

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Cavallaro, Antonino Giovanni. “Streptococcus agalactiae e colonizzazione materna: valutazione dei meccanismi di virulenza e chemioprofilassi.” 2011. Thesis, Università degli Studi di Catania. Accessed January 16, 2021. http://hdl.handle.net/10761/165.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Cavallaro, Antonino Giovanni. “Streptococcus agalactiae e colonizzazione materna: valutazione dei meccanismi di virulenza e chemioprofilassi.” 2011. Web. 16 Jan 2021.

Vancouver:

Cavallaro AG. Streptococcus agalactiae e colonizzazione materna: valutazione dei meccanismi di virulenza e chemioprofilassi. [Internet] [Thesis]. Università degli Studi di Catania; 2011. [cited 2021 Jan 16]. Available from: http://hdl.handle.net/10761/165.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Cavallaro AG. Streptococcus agalactiae e colonizzazione materna: valutazione dei meccanismi di virulenza e chemioprofilassi. [Thesis]. Università degli Studi di Catania; 2011. Available from: http://hdl.handle.net/10761/165

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Stirling

6. Featherstone, Zoe L. Investigations into the pathogenesis of aquatic Streptococcus agalactiae and Streptococcus iniae in Nile tilapia (Oreochromis niloticus).

Degree: PhD, 2014, University of Stirling

URL: http://hdl.handle.net/1893/21633

► The bacterial pathogens Streptococcus agalactiae and S. iniae have the capacity to infect a wide range of fish species throughout the world, with Nile tilapia… (more)

▼ The bacterial pathogens Streptococcus agalactiae and S. iniae have the capacity to infect a wide range of fish species throughout the world, with Nile tilapia (Oreochromis niloticus) being particularly susceptible. Global tilapia aquaculture production was estimated to be 3.5 million tonnes in 2008, and has a significant contribution in the global farmed fish market. Due to their ability to adapt to a wide range of culture systems the commercialisation of tilapia production has occurred in more than 100 countries. However, countries such as China have suffered from severe and extensive outbreaks of streptococcosis in cultured tilapia continuously for many years. Such large-scale outbreaks in China have resulted in a loss of approximately US$0.4 billion in 2011. Fish are permanently exposed to a plethora of pathogens and natural disease outbreaks are complex host-pathogen interactions that seldom involve single pathogen infections. As a consequence, simultaneous infections, alternatively called concurrent or co-infections, are starting to receive interest from aquatic disease researchers. Streptococcus agalactiae and S. iniae infections can both occur in the same geographic area and both S. agalactiae and S. iniae have been found to be present on the same farm in a single disease outbreak. It has been found that a disease outbreak caused by one these pathogens can be followed by another outbreak from the other. These two pathogens have serious effects on the tilapia aquaculture industry yet there is no information regarding S. agalactiae and S. iniae co-infections. Such information would be valuable for understanding epidemiology and the development of improved treatment and control of aquatic streptococcosis infections. The overall aim of this study was to investigate the pathogenesis of S. agalactiae and S. iniae in Nile tilapia. One important aspect of investigating simultaneous infections was to examine if there was any competition or synergy between S. agalactiae and S. iniae in vitro or in vivo. It was found that competition between S. agalactiae and S. iniae in vitro was inconsistent between different experimental systems. Results indicated that there was either no interaction between bacterial species or they coexisted during in vitro competition assays. Whereas, an in vivo model utilising wax moth larvae (Galleria mellonella) suggested that during a simultaneous infection with S. agalactiae and S. iniae the total levels of larval mortality were lower than expected indicating that the pathogens may have interacted with one another in a competitive manner. Investigations were also conducted to identify the expression of virulence factors in vitro for S. agalactiae and S. iniae. Comparisons were then made to ascertain any inter- and intra-species variation. Results demonstrated that both S. agalactiae and S. iniae strains possessed a capsule but varied in their haemolytic activity, blood survival and resistance to complement-mediated killing. These variations suggested that the two bacterial species…

Subjects/Keywords: Streptococcus agalactiae; Streptococcus iniae; Nile tilapia; Pathogenesis; Streptococcus agalactiae; Streptococcus; Nile tilapia; Pathogenesis; Fishes Diseases

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Featherstone, Z. L. (2014). Investigations into the pathogenesis of aquatic Streptococcus agalactiae and Streptococcus iniae in Nile tilapia (Oreochromis niloticus). (Doctoral Dissertation). University of Stirling. Retrieved from http://hdl.handle.net/1893/21633

Chicago Manual of Style (16^th Edition):

Featherstone, Zoe L. “Investigations into the pathogenesis of aquatic Streptococcus agalactiae and Streptococcus iniae in Nile tilapia (Oreochromis niloticus).” 2014. Doctoral Dissertation, University of Stirling. Accessed January 16, 2021. http://hdl.handle.net/1893/21633.

MLA Handbook (7^th Edition):

Featherstone, Zoe L. “Investigations into the pathogenesis of aquatic Streptococcus agalactiae and Streptococcus iniae in Nile tilapia (Oreochromis niloticus).” 2014. Web. 16 Jan 2021.

Vancouver:

Featherstone ZL. Investigations into the pathogenesis of aquatic Streptococcus agalactiae and Streptococcus iniae in Nile tilapia (Oreochromis niloticus). [Internet] [Doctoral dissertation]. University of Stirling; 2014. [cited 2021 Jan 16]. Available from: http://hdl.handle.net/1893/21633.

Council of Science Editors:

Featherstone ZL. Investigations into the pathogenesis of aquatic Streptococcus agalactiae and Streptococcus iniae in Nile tilapia (Oreochromis niloticus). [Doctoral Dissertation]. University of Stirling; 2014. Available from: http://hdl.handle.net/1893/21633

Michigan State University

7. Korir, Michelle Lynn. Variation in host-pathogen interactions among genetically diverse strains of Group B Streptococcus.

Degree: 2016, Michigan State University

URL: http://etd.lib.msu.edu/islandora/object/etd:4282

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Thesis Ph. D. Michigan State University. Microbiology and Molecular Genetics 2016

"Streptococcus agalactiae, or Group B Streptococcus (GBS), is a highly diverse species that can… (more)

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Thesis Ph. D. Michigan State University. Microbiology and Molecular Genetics 2016

"Streptococcus agalactiae, or Group B Streptococcus (GBS), is a highly diverse species that can be found asymptomatically colonizing the gastrointestinal and genital tracts of healthy adults, but is also capable of causing severe invasive disease. GBS is a leading cause of sepsis and meningitis in neonates and the only preventative measure is antibiotic therapy given to pregnant mothers during labor to prevent transmission. Although this method was effective at reducing case rates upon implementation, case rates have remained unchanged since the initial decline and some mothers remain persistently colonized by GBS. Due to the high level of diversity among strains it is important to understand how strains differ at the various stages of disease progression in order to have a more complete understanding of GBS pathogenesis. Here, I examined how genotypically diverse strains differ in their interactions with human cells. The examination of strains of the same serotype in ability to associate with decidual cells and lung epithelial cells revealed that strains within the same serotype, and even the same sequence type (ST) differed in attachment and invasion, but this variation was dependent on host cell type. More specifically, strains of the hypervirulent lineage, ST-17 associated with decidual cells significantly more than the other STs, but the opposite was true for lung epithelial cell attachment. Mechanisms of persistent colonization was explored by comparing antibiotic tolerance and macrophage survival between ST-17 and ST-12 strains, which persisted and was eradicated after antibiotic prophylaxis, respectively. This study revealed that although the ST-17 strain was not tolerant to antibiotics, subinhibitory antibiotics enhanced phagocytic uptake of this strain where it was able to survive for an extended period of time. Additionally, intracellular survival of the ST-17 strain was dependent on acidification of the phagosome, whereas altered pH had no effect on survival of the ST12 strain, suggesting GBS can use different mechanisms of survival. Moreover, serotype III GBS strains were better able to survive phagosomal stress compared to other serotypes. Lastly, transcriptome analysis of the ST-17 strain during intracellular survival revealed temporal gene expression responses to long term survival and identified a large number of factors important for intracellular survival. Through mutagenesis studies, the roles of NADH peroxidase (Npx) and cadmium resistance protein (CadD) in GBS intracellular survival was examined. These studies demonstrated that Npx promotes resistance to reactive oxygen stress through detoxification of hydrogen peroxide and CadD serves as a heavy metal efflux pump to confer resistance to intoxication by certain divalent metal cations. The work described here reveals new insights in GBS pathogenesis and helps identify key virulence factors that can serve as targets for alternative therapeutics and vaccine…

Advisors/Committee Members: Manning, Shannon, Waters, Christopher, Mansfield, Linda, Abramovitch, Robert.

Subjects/Keywords: Streptococcus agalactiae – Pathogenesis; Streptococcus agalactiae – Genetic aspects; Host-parasite relationships; Microbiology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Korir, M. L. (2016). Variation in host-pathogen interactions among genetically diverse strains of Group B Streptococcus. (Thesis). Michigan State University. Retrieved from http://etd.lib.msu.edu/islandora/object/etd:4282

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Korir, Michelle Lynn. “Variation in host-pathogen interactions among genetically diverse strains of Group B Streptococcus.” 2016. Thesis, Michigan State University. Accessed January 16, 2021. http://etd.lib.msu.edu/islandora/object/etd:4282.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Korir, Michelle Lynn. “Variation in host-pathogen interactions among genetically diverse strains of Group B Streptococcus.” 2016. Web. 16 Jan 2021.

Vancouver:

Korir ML. Variation in host-pathogen interactions among genetically diverse strains of Group B Streptococcus. [Internet] [Thesis]. Michigan State University; 2016. [cited 2021 Jan 16]. Available from: http://etd.lib.msu.edu/islandora/object/etd:4282.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Korir ML. Variation in host-pathogen interactions among genetically diverse strains of Group B Streptococcus. [Thesis]. Michigan State University; 2016. Available from: http://etd.lib.msu.edu/islandora/object/etd:4282

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

8. Pinheiro, Sandra Marisa de Oliveira Sequeira. Caracterização Genética da Resistência à Eritromicina em Streptococcus agalactia e Degestantes saudáveis.

Degree: 2009, RCAAP

URL: http://www.rcaap.pt/detail.jsp?id=oai:http://repositorio.utad.pt/:10348/419

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Dissertação de Mestrado em Biologia Clínica Laboratorial

Streptococcus agalactiae (Grupo B de Lancefield, EGB), um microrganismo comensal do homem, reconhecido em 1920 como o agente… (more)

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Dissertação de Mestrado em Biologia Clínica Laboratorial

Streptococcus agalactiae (Grupo B de Lancefield, EGB), um microrganismo comensal do homem, reconhecido em 1920 como o agente etiológico da mastite bovina, tem sido associado a infecções em parturientes e recém-nascidos, sendo o principal agente de septicemia e meningite neonatal. Para evitar a infecção perinatal recomenda-se a pesquisa da bactéria na região vagino-perianal durante o terceiro semestre de gravidez, indicando o tratamento antibiótico durante o parto das gestantes colonizadas. O objectivo deste estudo foi verificar a ocorrência de colonização por Streptococcus agalactiae em gestantes saudáveis, avaliar a associação da colonização com variáveis maternas demográficas e clínicas, avaliar a susceptibilidade dos isolados aos antibióticos e determinar os fenótipos de resistência à eritromicina e à clindamicina e os mecanismos genéticos nelas implicados, com utilidade clínica na recomendação da profilaxia e terapêutica empírica. Realizou-se um estudo envolvendo 520 gestantes em acompanhamento pré-natal, atendidas no Centro Hospitalar de Trás-os-Montes e Alto Douro, durante o período de Janeiro a Junho de 2008. Durante o atendimento foram colhidas amostras vaginal e anorrectal para rastrear a presença de colonização por S. agalactiae. As gestantes colonizadas por EGB foram caracterizadas por variáveis demográficas (idade e número de gestações) e clínicas (ocorrência de abortos anteriores e presença ou ausência de diabetes mellitus). As colheitas foram efectuadas com zaragatoas estéreis e colocadas em meio selectivo de Todd Hewitt. Procederam-se às provas presuntivas de identificação de EGB (coloração pelo método de Gram, observação microscópica, prova da catalase), e provas confirmatórias (CAMP e aglutinação em látex). Realizou-se o estudo da resistência aos antibióticos eritromicina, clindamicina, penicilina, cefotaxima, vancomicina, quinupristina-dalfopristina, cloranfenicol, ofloxacina e meropenem. O E-teste para o cálculo das concentrações mínimas inibitórias (mg/l) foi aplicado, adicionalmente, para a eritromicina e clindamicina. Os fenótipos de resistência MLSB induzida e constitutiva (cMLSB e iMLSB, respectivamente), bem como o fenótipo de resistência M da eritromicina e clindamicina foram determinados pelo teste de disco duplo. A presença dos genes que conferem resistência aos macrólidos como ermA, ermB, ermC, ermTR, msrA e mef(A/E) foram estudados através de PCR, com o uso de primers específicos e posterior sequenciação. A prevalência de colonização materna por este microrganismo foi de 18% na população estudada. Todos os 93 isolados mostraram susceptibilidade à penicillina, cefotaxima, vancomicina, quinupristina-dalfopristina, cloranfenicol, ofloxacina e meropenem. A resistência à eritromicina e clindamicina foi detectada em 15% e 9,6% dos isolados, respectivamente. Os fenótipos de resistência detectados entre os 14 isolados resistentes à eritromicina foram os seguintes (número de isolados): cMLSB (9), iMLSB (3), e de M (2). As CMI (mg/l) para…

Advisors/Committee Members: Poeta, Patrícia, Igrejas, Gilberto.

Subjects/Keywords: Streptococcus agalactiae; gravidez; antibióticos; eritromicina; genótipos

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Pinheiro, S. M. d. O. S. (2009). Caracterização Genética da Resistência à Eritromicina em Streptococcus agalactia e Degestantes saudáveis. (Thesis). RCAAP. Retrieved from http://www.rcaap.pt/detail.jsp?id=oai:http://repositorio.utad.pt/:10348/419

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Pinheiro, Sandra Marisa de Oliveira Sequeira. “Caracterização Genética da Resistência à Eritromicina em Streptococcus agalactia e Degestantes saudáveis.” 2009. Thesis, RCAAP. Accessed January 16, 2021. http://www.rcaap.pt/detail.jsp?id=oai:http://repositorio.utad.pt/:10348/419.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Pinheiro, Sandra Marisa de Oliveira Sequeira. “Caracterização Genética da Resistência à Eritromicina em Streptococcus agalactia e Degestantes saudáveis.” 2009. Web. 16 Jan 2021.

Vancouver:

Pinheiro SMdOS. Caracterização Genética da Resistência à Eritromicina em Streptococcus agalactia e Degestantes saudáveis. [Internet] [Thesis]. RCAAP; 2009. [cited 2021 Jan 16]. Available from: http://www.rcaap.pt/detail.jsp?id=oai:http://repositorio.utad.pt/:10348/419.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Pinheiro SMdOS. Caracterização Genética da Resistência à Eritromicina em Streptococcus agalactia e Degestantes saudáveis. [Thesis]. RCAAP; 2009. Available from: http://www.rcaap.pt/detail.jsp?id=oai:http://repositorio.utad.pt/:10348/419

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Michigan State University

9. Springman, Amber Cody. Genetic diversity and virulence gene characterization of Group B Streptococcus.

Degree: 2012, Michigan State University

URL: http://etd.lib.msu.edu/islandora/object/etd:769

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Thesis Ph. D. Michigan State University. Comparative Medicine and Integrative Biology 2012.

Group B Streptococcus (GBS, Streptococcus agalactiae) is a Gram-positive, beta-hemolytic bacterium that was… (more)

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Thesis Ph. D. Michigan State University. Comparative Medicine and Integrative Biology 2012.

Group B Streptococcus (GBS, Streptococcus agalactiae) is a Gram-positive, beta-hemolytic bacterium that was first described as a causative agent in bovine mastitis. GBS has since become a leading cause of pneumonia, sepsis, and meningitis newborns and has also emerged as an opportunistic pathogen in the elderly and adults with underlying medical conditions. The accessibility to a collection of GBS strains from colonized women, newborns, adults, elderly, and bovines has provided an excellent basis for conducting molecular epidemiological studies. The use of molecular subtyping methods including multilocus sequence typing (MLST), PCR-based RFLP, and mulitiplex PCR has allowed for inferences into the phylogenetic relationships among strains and the identification of specific virulence determinants associated with different clonal lineages. During the first study of this dissertation, the extent of genetic diversity and allelic variation within key virulence genes were analyzed in a panel of 94 GBS strains from diverse sources (e.g. pregnant women, newborns, elderly adults, and bovines). In the second study, a more inclusive set of 295 human-derived and bovine-derived strains representing 73 sequence types were evaluated for the presence of and variation in horizontally acquired pilus islands (PIs). Extensive genetic analyses conducted in these studies revealed that recombination and horizontal gene transfer have played a major role in the diversification of GBS clonal lineages. Furthermore, evolutionary pressures have favored the selection of specific allelic determinants that has led to the emergence of highly specialized lineages that have become successful at causing disease in humans. Overall, this research has strong implications for vaccine development since many of the virulence genes examined herein have been proposed as vaccine candidates. The high degree of sequence variability observed in many of the potential vaccine targets underscores the difficulty of developing a broadly protective, universal vaccine against GBS. Continued efforts to assess the genetic diversity of GBS populations and the mechanisms involved in pathogenesis will prove critical for establishing new prevention measures to combat GBS-associated disease.

Description based on online resource; title from PDF t.p. (ProQuest, viewed Oct. 23, 2013)

Advisors/Committee Members: Manning, Shannon D, Davies, H. Dele, Mansfield, Linda, Marsh, Terrence.

Subjects/Keywords: Streptococcus agalactiae; Virulence (Microbiology) – Genetic aspects; Microbiology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Springman, A. C. (2012). Genetic diversity and virulence gene characterization of Group B Streptococcus. (Thesis). Michigan State University. Retrieved from http://etd.lib.msu.edu/islandora/object/etd:769

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Springman, Amber Cody. “Genetic diversity and virulence gene characterization of Group B Streptococcus.” 2012. Thesis, Michigan State University. Accessed January 16, 2021. http://etd.lib.msu.edu/islandora/object/etd:769.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Springman, Amber Cody. “Genetic diversity and virulence gene characterization of Group B Streptococcus.” 2012. Web. 16 Jan 2021.

Vancouver:

Springman AC. Genetic diversity and virulence gene characterization of Group B Streptococcus. [Internet] [Thesis]. Michigan State University; 2012. [cited 2021 Jan 16]. Available from: http://etd.lib.msu.edu/islandora/object/etd:769.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Springman AC. Genetic diversity and virulence gene characterization of Group B Streptococcus. [Thesis]. Michigan State University; 2012. Available from: http://etd.lib.msu.edu/islandora/object/etd:769

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Federal do Maranhão

10. ADRIANA LIMA DOS REIS COSTA. COLONIZAÇÃO PELO ESTREPTOCOCO DO GRUPO B EM GESTANTES DURANTE O TRABALHO DE PARTO EM UMA MATERNIDADE DE SÃO LUÍS, MARANHÃO.

Degree: 2008, Universidade Federal do Maranhão

URL: http://www.tedebc.ufma.br//tde_busca/arquivo.php?codArquivo=257

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Objetivo: Estudar a colonização pelo estreptococo do grupo B (EGB) em gestantes em pródromos ou em trabalho de parto. Métodos: Foram colhidas culturas vaginal e… (more)

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Objetivo: Estudar a colonização pelo estreptococo do grupo B (EGB) em gestantes em pródromos ou em trabalho de parto. Métodos: Foram colhidas culturas vaginal e retal de 201 gestantes atendidas na Admissão/SPA da Maternidade Marly Sarney em São Luís MA. As amostras obtidas foram inoculadas em meio seletivo de Todd- Hewith e, posteriormente subcultivadas em placas de ágar-sangue. O teste de CAMP foi utilizado para identificação do EGB. Foram estudadas as variáveis sóciodemográficas e antecedentes gineco-obstétricos e desfechos perinatais. As amostras positivas para EGB foram submetidas a teste de sensibilidade para antibióticos. Resultados: A prevalência da colonização materna pelo EGB foi de 20,39%. Não foi encontrada associação entre as variáveis sócio-demográficas ou antecedentes gineco-obstétricos com a maior presença de colonização pelo EGB. Houve dois desfechos infecciosos entre os recém-natos de mães colonizadas, porém as hemoculturas foram negativas. Foram encontradas taxas de resistência elevadas para os seguintes antibióticos: clindamicina 25,45%, eritromicina 23,63% e ceftriaxona 12,72%. Conclusão: As evidências apontam para a presença de altas taxas de colonização materna pelo EGB que indicariam a pesquisa sistemática deste agente entre as gestantes.

Objective: To study the colonization with group B streptococcus (GBS) in pregnant women near or in time for delivery. Methods: Vaginal and rectal cultures of 201 pregnant women were collected in the Marly Sarney Maternity in São Luís Maranhão, Brazil. The samples had been inoculated in Todd- Hewith selective media and then subcultured in plates of Agar-blood. The CAMP test was used for identification of the EGB. There were studied some demographic variables, obstetric antecedent and perinatal outcomes. The positive samples for EGB had been submitted to testing of sensitivity for antibiotics. Results: The prevalence of the maternal colonization with GBS was of 20.39%. There were not found association between the demographic variables and obstetric antecedents with higher presence of colonization with the EGB. There were two infectious outcomes between the newborns of colonized mothers however the hemocultures had been negative. Raised taxes of resistance for following antibiotics had been found: clindamicina 25.45%, eritromicina 23.63% and ceftriaxona 12.72%. Conclusion: The evidences point the presence of high taxes of maternal colonization with GBS that would indicate the systematic research of this agent among the pregnant women.

Advisors/Committee Members: Fernando Lamy Filho.

Subjects/Keywords: Prevalence; Colonização; SAUDE MATERNO-INFANTIL; Prevalência; Colonization; Pregnancy; Streptococcus agalactiae; Streptococcus agalactiae; Gravidez

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

COSTA, A. L. D. R. (2008). COLONIZAÇÃO PELO ESTREPTOCOCO DO GRUPO B EM GESTANTES DURANTE O TRABALHO DE PARTO EM UMA MATERNIDADE DE SÃO LUÍS, MARANHÃO. (Thesis). Universidade Federal do Maranhão. Retrieved from http://www.tedebc.ufma.br//tde_busca/arquivo.php?codArquivo=257

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

COSTA, ADRIANA LIMA DOS REIS. “COLONIZAÇÃO PELO ESTREPTOCOCO DO GRUPO B EM GESTANTES DURANTE O TRABALHO DE PARTO EM UMA MATERNIDADE DE SÃO LUÍS, MARANHÃO.” 2008. Thesis, Universidade Federal do Maranhão. Accessed January 16, 2021. http://www.tedebc.ufma.br//tde_busca/arquivo.php?codArquivo=257.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

COSTA, ADRIANA LIMA DOS REIS. “COLONIZAÇÃO PELO ESTREPTOCOCO DO GRUPO B EM GESTANTES DURANTE O TRABALHO DE PARTO EM UMA MATERNIDADE DE SÃO LUÍS, MARANHÃO.” 2008. Web. 16 Jan 2021.

Vancouver:

COSTA ALDR. COLONIZAÇÃO PELO ESTREPTOCOCO DO GRUPO B EM GESTANTES DURANTE O TRABALHO DE PARTO EM UMA MATERNIDADE DE SÃO LUÍS, MARANHÃO. [Internet] [Thesis]. Universidade Federal do Maranhão; 2008. [cited 2021 Jan 16]. Available from: http://www.tedebc.ufma.br//tde_busca/arquivo.php?codArquivo=257.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

COSTA ALDR. COLONIZAÇÃO PELO ESTREPTOCOCO DO GRUPO B EM GESTANTES DURANTE O TRABALHO DE PARTO EM UMA MATERNIDADE DE SÃO LUÍS, MARANHÃO. [Thesis]. Universidade Federal do Maranhão; 2008. Available from: http://www.tedebc.ufma.br//tde_busca/arquivo.php?codArquivo=257

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

11. Mário Sérgio Silva Gomes Caetano. Colonização pelo Streptococcus agalactiae (EGB) em gestantes atendidas na rede pública de Uberaba - MG.

Degree: 2008, Universidade Federal do Triângulo Mineiro

URL: http://bdtd.uftm.edu.br/tde_busca/arquivo.php?codArquivo=48

► Introdução: Estima-se que 10 a 30% das mulheres grávidas estejam colonizadas pelo Streptococcus agalactiae (EGB). Esse microorganismo pode ser transmitido no momento do parto e… (more)

▼ Introdução: Estima-se que 10 a 30% das mulheres grávidas estejam colonizadas pelo Streptococcus agalactiae (EGB). Esse microorganismo pode ser transmitido no momento do parto e representa importante causa de sepse neonatal. No Brasil, não há, até o momento, estratégia definida para a profilaxia da infecção pelo EGB. Dessa forma, o conhecimento da prevalência da colonização pelo EGB em gestantes é importante passo na adoção da profilaxia da infecção causada por esse microorganismo. Objetivo: Detectar a presença de EGB em amostras coletadas da vagina e da região anorretal de gestantes, determinar a prevalência da colonização pelo EGB nessa população e conhecer o perfil de sensibilidade aos antimicrobianos das cepas isoladas. Método: Realizou-se um estudo de corte transversal, envolvendo trezentas gestantes em atendimento pré-natal no Ambulatório Maria da Glória da Universidade Federal do Triângulo Mineiro (AMG-UFTM) e no Centro Integrado à Saúde da Mulher (CAISM). Durante o atendimento, foram coletadas amostras, vaginal e anorretal, dessas gestantes com swabs estéreis e colocados em meio seletivo de Todd Hewitt. Dados sociodemográficos foram obtidos nesse momento e as informações clínicas pesquisadas nos prontuários. Procederam-se provas presuntivas de identificação do EGB (coloração pelo método de Gram, catalase, sensibilidade a sufametoxazol-trimetoprim e bacitracina, provas da bile-esculina e NaCl a 6,5%) e provas confirmatórias (CAMP e aglutinação em látex). Realizou-se susceptibilidade a oito antimicrobianos (penicilina, ampicilina, eritromicina, nitrofurantoína, clindamicina, cefalotina, gentamicina e vancomicina) através 20 da técnica de disco difusão. Resultado: A prevalência da colonização de gestantes pelo EGB foi de 15%; dentre 22 as variáveis sociodemográficas a renda familiar foi um fator significativo associado à 23 colonização. Todas as cepas foram sensíveis à penicilina ampicilina, à cefalotina e à 24 vancomicina e resistentes à gentamicina. Ocorreu resistência à eritromicina e à clindamicina em 2,2% das amostras testadas e para a nitrofurantoína em 4,4% das 26 amostras. Conclusões: A prevalência da colonização pelo EGB em gestantes deste estudo está concordante com os demais estudos brasileiros, nos quais se empregou a mesma metodologia. Não se identificaram fatores associados à colonização, exceto a renda familiar. A penicilina, em virtude da sua sensibilidade, ainda é a droga de escolha. O antibiograma é necessário para as pacientes que referiram reações alérgicas prévias a essa medicação. Advisors/Committee Members: Adriana Gonçalves de Oliveira, Marina Carvalho Paschoini, Roseli Aparecida da Silva Gomes.

Subjects/Keywords: Streptococcus agalactiae; Infecções estreptocócicas; Gravidez; Infecção; ANATOMIA PATOLOGICA E PATOLOGIA CLINICA; Streptococcus agalactiae; Infection; Pregnancy

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Caetano, M. S. S. G. (2008). Colonização pelo Streptococcus agalactiae (EGB) em gestantes atendidas na rede pública de Uberaba - MG. (Thesis). Universidade Federal do Triângulo Mineiro. Retrieved from http://bdtd.uftm.edu.br/tde_busca/arquivo.php?codArquivo=48

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Caetano, Mário Sérgio Silva Gomes. “Colonização pelo Streptococcus agalactiae (EGB) em gestantes atendidas na rede pública de Uberaba - MG.” 2008. Thesis, Universidade Federal do Triângulo Mineiro. Accessed January 16, 2021. http://bdtd.uftm.edu.br/tde_busca/arquivo.php?codArquivo=48.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Caetano, Mário Sérgio Silva Gomes. “Colonização pelo Streptococcus agalactiae (EGB) em gestantes atendidas na rede pública de Uberaba - MG.” 2008. Web. 16 Jan 2021.

Vancouver:

Caetano MSSG. Colonização pelo Streptococcus agalactiae (EGB) em gestantes atendidas na rede pública de Uberaba - MG. [Internet] [Thesis]. Universidade Federal do Triângulo Mineiro; 2008. [cited 2021 Jan 16]. Available from: http://bdtd.uftm.edu.br/tde_busca/arquivo.php?codArquivo=48.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Caetano MSSG. Colonização pelo Streptococcus agalactiae (EGB) em gestantes atendidas na rede pública de Uberaba - MG. [Thesis]. Universidade Federal do Triângulo Mineiro; 2008. Available from: http://bdtd.uftm.edu.br/tde_busca/arquivo.php?codArquivo=48

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Kaunas University of Medicine

12. Tamelienė, Rasa. Naujagimių kolonizacijos B grupės beta hemoliziniu streptokoku ir Escherichia coli įtaka naujagimių ankstyvam infekciniam sergamumui.

Degree: Dissertation, Medicine, 2010, Kaunas University of Medicine

URL: http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2010~D_20100708_094303-64540 ;

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Naujagimių sepsis yra viena pagrindinių šios amžiaus grupės vaikų sergamumo, ir mirtingumo priežasčių. Pagrindiniai ankstyvojo naujagimių sepsio sukėlėjai yra B grupės beta hemolizinis streptokokas ir… (more)

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Naujagimių sepsis yra viena pagrindinių šios amžiaus grupės vaikų sergamumo, ir mirtingumo priežasčių. Pagrindiniai ankstyvojo naujagimių sepsio sukėlėjai yra B grupės beta hemolizinis streptokokas ir E.coli. 10-40 proc. moterų BGS nešioja makštyje ir iki gimdymo arba jo metu perduoda BGS savo naujagimiui. Klinikiniais tyrimais įrodžius, kad antibiotikų skyrimas gimdymo metu BGS kolonizuotoms moterims mažina ankstyvojo BGS sukelto naujagimių sepsio dažnumą, buvo priimtos ankstyvosios naujagimių infekcijos prevencijos rekomendacijos. Tačiau nėra vieningos nuomonės dėl prevencijos atlikimo metodikos. Vienose šalyse antibiotikų gimdymo metu skiriama 35 –37 nėštumo savaitę nešiojančioms BGS makštyje moterims, kitose – gimdymo metu atsiradus ankstyvosios infekcijos rizikos veiksnių. Pastaruoju metu pasirodo epidemiologinių tyrimų duomenys, kad naudojant antibiotikų profilaktiką BGS sukelto sepsio prevencijai, dažnėja kitų motinos makštyje nešiojamų ir naujagimiui perduodamų sukėlėjų – E. coli sukeliamų infekcijų.Taigi, svarbu žinoti kiekvienos populiacijos, taip pat ir Lietuvos, moterų BGS nešiojimo bei naujagimių kolonizacijos dažnį bei ankstyvosios naujagimių infekcijos dažnį, kad galima būtų pasirinkti, kokia prevencijos metodika šalyje reikėtų naudotis. Atliktas tyrimas yra pirmas, kuriame kartu nagrinėjami dviejų sukėlėjų – BGS ir E. coli – kolonizacijos dažnis, rizikos veiksniai, perdavimas naujagimiams bei šių mikroorganizmų sukelto sepsio dažnis.

Bacterial sepsis is a major cause of morbidity and mortality in children. Early onset neonatal sepsis is a major contributor to these sequelae. Group B Streptococcus and E.coli emerged dramatically as the leading cause of invasive neonatal infection. The major risk factor for early onset GBS sepsis in neonates is maternal intrapartum GBS colonization. The prevalence of maternal recto-vaginal colonization ranges from 15 to 40 % and GBS colonizes the infant during labour and delivery. In the 1980s, clinical trials demonstrated that intrapartum intravenous antibiotics (IAP) could prevent early onset neonatal GBS sepsis. The guidelines for the prevention of neonatal GBS disease were published recommending IAP on the basis of screening-based or risk-based strategies. It is of importance to know the maternal intrapartum GBS colonization frequency in every population, that of Lithuania included, as well as the frequency of early onset GBS infection in neonates so that the best preferable methods of prevention in the country could be applied. These data are not available in Lithuania at present and IAP has not been standardized yet. Our study therefore will be the first attempt specially devoted to the study of two pathogens, i. e. the GBS and E. coli colonization frequency, risk factors and transmission mechanisms of these pathogens to neonates, as well as the frequency of sepsis caused by these bacteria.

Advisors/Committee Members: Nadišauskienė, Rūta Jolanta (Doctoral dissertation supervisor), Markūnienė, Eglė (Doctoral dissertation advisor), Barauskas, Vidmantas (Doctoral dissertation committee chair), Verkauskienė, Rasa (Doctoral dissertation committee member), Žilinskas, Henrikas (Doctoral dissertation committee member), Pavilonis, Alvydas (Doctoral dissertation committee member), Starkuvienė, Skirmantė (Doctoral dissertation opponent), Usonis, Vytautas (Doctoral dissertation opponent), Šiugždaitė, Jūratė (Doctoral dissertation committee member).

Subjects/Keywords: Streptococcus agalactiae; Sepsis; Naujagimis; Kolonizacija; Antibiotikų profilaktika; Streptococcus agalactiae; Sepsis; Neonate; Colonization; Antibiotic propxylaxis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Tamelienė, R. (2010). Naujagimių kolonizacijos B grupės beta hemoliziniu streptokoku ir Escherichia coli įtaka naujagimių ankstyvam infekciniam sergamumui. (Doctoral Dissertation). Kaunas University of Medicine. Retrieved from http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2010~D_20100708_094303-64540 ;

Chicago Manual of Style (16^th Edition):

Tamelienė, Rasa. “Naujagimių kolonizacijos B grupės beta hemoliziniu streptokoku ir Escherichia coli įtaka naujagimių ankstyvam infekciniam sergamumui.” 2010. Doctoral Dissertation, Kaunas University of Medicine. Accessed January 16, 2021. http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2010~D_20100708_094303-64540 ;.

MLA Handbook (7^th Edition):

Tamelienė, Rasa. “Naujagimių kolonizacijos B grupės beta hemoliziniu streptokoku ir Escherichia coli įtaka naujagimių ankstyvam infekciniam sergamumui.” 2010. Web. 16 Jan 2021.

Vancouver:

Tamelienė R. Naujagimių kolonizacijos B grupės beta hemoliziniu streptokoku ir Escherichia coli įtaka naujagimių ankstyvam infekciniam sergamumui. [Internet] [Doctoral dissertation]. Kaunas University of Medicine; 2010. [cited 2021 Jan 16]. Available from: http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2010~D_20100708_094303-64540 ;.

Council of Science Editors:

Tamelienė R. Naujagimių kolonizacijos B grupės beta hemoliziniu streptokoku ir Escherichia coli įtaka naujagimių ankstyvam infekciniam sergamumui. [Doctoral Dissertation]. Kaunas University of Medicine; 2010. Available from: http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2010~D_20100708_094303-64540 ;

University of Stirling

13. Wongsathein, Dilok. Factors affecting experimental Streptococcus agalactiae infection in tilapia, Oreochromis niloticus.

Degree: PhD, School of Natural Sciences, 2012, University of Stirling

URL: http://hdl.handle.net/1893/10375

► Streptococcus agalactiae infection is one of the major disease problems affecting farmed tilapia (Oreochromis niloticus) worldwide. Tilapia are highly susceptible to this disease which results… (more)

▼ Streptococcus agalactiae infection is one of the major disease problems affecting farmed tilapia (Oreochromis niloticus) worldwide. Tilapia are highly susceptible to this disease which results in mortality of up to 70% over a period of around 7 days and significant economic losses for farmers. Affected tilapia commonly present with an irregular behaviour associated with meningoencephalitis and septicaemia. Currently, factors affecting the virulence and transmission of S. agalactiae in fish including tilapia are poorly understood. Reports from natural outbreaks of S. agalactiae infection on tilapia farms have suggested larvae and juvenile or fish smaller than 20 g are not susceptible. In addition, there is variability in individual response to experimental inflammatory challenge associated with coping styles (bold, shy) in common carp (Cyprinus carpio). The central hypotheses of this thesis were that weight, age and coping style might affect the development and progression of this bacterial disease. This study investigated these three factors with experimental S. agalactiae infection in Nile tilapia. A range of bacterial isolates recovered from farmed tilapia, presenting with clinical sign of streptococcosis during natural disease outbreaks were identified and characterised as S. agalactiae by standard conventional methods, biochemical characteristic tests, Lancefield serogrouping and species-specific PCR assay. These isolates were Gram-positive cocci, either β- or non-haemolytic (γ), non-motile, oxidase negative and all of serogroup B. In addition, they were able to grow on Edwards medium (modified) agar as blue colonies and growth was observed in broth from 22 to 37 °C and with 0.5-5% NaCl. The biochemical profiles showed some differences in reactions while all the PCR samples showed similarities to the S. agalactiae type strain. These data confirmed that these strains were identified as group B S. agalactiae. A challenge model for S. agalactiae in Nile tilapia was developed and the LD50 estimated prior to performing subsequent experimental challenge studies. Two exposure routes, immersion and intraperitoneal injection (i.p.), were tested with various concentrations of S. agalactiae. Only i.p. injection produced significant mortalities (9 × 108 CFU/ml = 48% mortality, 9 × 107 = 48% and 8 × 106 = 26%). Streptococcus agalactiae was recovered and identified from all the dead and moribund fish during these experiments, where affected fish showed similar clinical signs and pathology to those reported from natural S. agalactiae infections. The study results showed that an experimental i.p. challenge model for S. agalactiae infection had successfully infected healthy Nile tilapia. In the immersion challenges, only 1 fish died despite testing a range of bacterial concentrations, exposure times, stocking density, water system and bacterial preparations. The experimental studies were conducted to investigate the association between weight or age of fish and susceptibility to S. agalactiae infection in Nile…

Subjects/Keywords: Streptococcus agalactiae; tilapia; factor; Tilapia; Streptococcus; Fish Diseases diagnosis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wongsathein, D. (2012). Factors affecting experimental Streptococcus agalactiae infection in tilapia, Oreochromis niloticus. (Doctoral Dissertation). University of Stirling. Retrieved from http://hdl.handle.net/1893/10375

Chicago Manual of Style (16^th Edition):

Wongsathein, Dilok. “Factors affecting experimental Streptococcus agalactiae infection in tilapia, Oreochromis niloticus.” 2012. Doctoral Dissertation, University of Stirling. Accessed January 16, 2021. http://hdl.handle.net/1893/10375.

MLA Handbook (7^th Edition):

Wongsathein, Dilok. “Factors affecting experimental Streptococcus agalactiae infection in tilapia, Oreochromis niloticus.” 2012. Web. 16 Jan 2021.

Vancouver:

Wongsathein D. Factors affecting experimental Streptococcus agalactiae infection in tilapia, Oreochromis niloticus. [Internet] [Doctoral dissertation]. University of Stirling; 2012. [cited 2021 Jan 16]. Available from: http://hdl.handle.net/1893/10375.

Council of Science Editors:

Wongsathein D. Factors affecting experimental Streptococcus agalactiae infection in tilapia, Oreochromis niloticus. [Doctoral Dissertation]. University of Stirling; 2012. Available from: http://hdl.handle.net/1893/10375

Universidade Nova

14. Rato, Márcia Alexandra Gonçalves. Epidemiological characterization, antimicrobial resistance and virulence mechanisms of human and animal streptococci.

Degree: 2011, Universidade Nova

URL: http://www.rcaap.pt/detail.jsp?id=oai:run.unl.pt:10362/7501

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Dissertação para obtenção do Grau de Doutor em Biologia

Streptococcus agalactiae (Group B Streptococcus - GBS), Streptococcus dysgalactiae subsp. dysgalactiae (Group C Streptococcus - GCS)… (more)

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Dissertação para obtenção do Grau de Doutor em Biologia

Streptococcus agalactiae (Group B Streptococcus - GBS), Streptococcus dysgalactiae subsp. dysgalactiae (Group C Streptococcus - GCS) and Streptococcus uberis are relevant mastitis pathogens, a highly prevalent and costly disease in the dairy industry due to antibiotherapy and loss in milk production. However, molecular characterization of field isolates of Streptococcus spp. occurring in Portugal was not known prior to our studies and is important to improve therapeutic and disease control programs. The aims of this study were the identification of strain molecular features, and the evaluation of antimicrobial drug resistance patterns of S. agalactiae (n=60), S. dysgalactiae subsp. dysgalactiae (n=18) and S. uberis (n=30) collected from bovine subclinical mastitis between 2002/2003 in Portugal. Additionally, two S. dysgalactiae subsp. dysgalactiae strains associated with invasive disease(one collected from cattle and the other from a human), and six Streptococcus dysgalactiae subsp. equisimilis (group C or group G Streptococcus - GCS/GGS) strains from human infection were included in the study, for comparative purposes. Genotypic relationships were identified using pulsed-field gel electrophoresis (PFGE)/BioNumerics, S. agalactiae and S. uberis multi-locus sequence typing (MLST), macrolide and tetracycline resistance gene profiling, S. agalactiae molecular serotyping, virulence gene profiling, PCR-amplification for screening presence of specific genes and subsequent sequencing for phylogenetic analysis, and reverse transcriptase-PCR (RT-PCR) for gene expression analysis of selected genes. Also, a custom-designed microarray containing 220 virulence genes of the human pathogen Streptococcus pyogenes (Group A Streptococcus - GAS) was used to test bovine GCS S. dysgalactiae subsp. dysgalactiae and human GCS/GGS S.dysgalactiae subsp. equisimilis. Antimicrobial resistance was assessed by disk diffusion against penicillin, gentamicin, streptomycin, amoxicillin-clavulanic acid, cefazolin, cefoperazone,rifaximin, erythromycin, pirlimycin, tetracycline, vancomycin, chloramphenicol and the macrolide lincosamide resistance phenotypes (cMLSB, iMLSB, M, L). Among S. uberis three PFGE clonal groups (defined by at least 80% similarity) comprised almost half of total isolates, and 50% of GBS isolates were included in four major clonal groups (all farm-associated), which is indicative of a contagious route of transmission between animals. The occurrence of PFGE patterns sharing >82.8% and 100% similarity among S. dysgalactiae subsp. dysgalactiae isolates collected from different farms suggests an environmental source for this pathogen in our case. By MLST, we observed that all S. uberis sequence types (ST) were found to be novel (n=14), representing novel genomic backgrounds for this pathogen. Among GBS only three MLST lineages (ST-2, ST-23, and ST-61/ST-554) were detected revealing little heterogeneity among our bovine GBS collection. Five new cpsD-cpsE-cpsF sequences of the…

Advisors/Committee Members: Sanches, Ilda, Vilela, Cristina.

Subjects/Keywords: Streptococcus agalactiae; Streptococcus dysgalactiae subsp. dysgalactiae; Streptococcus uberis; Molecular typing; Antimicrobial resistance; Virulence

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Rato, M. A. G. (2011). Epidemiological characterization, antimicrobial resistance and virulence mechanisms of human and animal streptococci. (Thesis). Universidade Nova. Retrieved from http://www.rcaap.pt/detail.jsp?id=oai:run.unl.pt:10362/7501

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Rato, Márcia Alexandra Gonçalves. “Epidemiological characterization, antimicrobial resistance and virulence mechanisms of human and animal streptococci.” 2011. Thesis, Universidade Nova. Accessed January 16, 2021. http://www.rcaap.pt/detail.jsp?id=oai:run.unl.pt:10362/7501.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Rato, Márcia Alexandra Gonçalves. “Epidemiological characterization, antimicrobial resistance and virulence mechanisms of human and animal streptococci.” 2011. Web. 16 Jan 2021.

Vancouver:

Rato MAG. Epidemiological characterization, antimicrobial resistance and virulence mechanisms of human and animal streptococci. [Internet] [Thesis]. Universidade Nova; 2011. [cited 2021 Jan 16]. Available from: http://www.rcaap.pt/detail.jsp?id=oai:run.unl.pt:10362/7501.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Rato MAG. Epidemiological characterization, antimicrobial resistance and virulence mechanisms of human and animal streptococci. [Thesis]. Universidade Nova; 2011. Available from: http://www.rcaap.pt/detail.jsp?id=oai:run.unl.pt:10362/7501

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

15. Ulisses de PÃdua Pereira. Genotipagem de Streptococcus agalactiae isolados de seres humanos, bovinos e peixes e seu potencial de virulÃncia para tilÃpia do Nilo.

Degree: 2008, UNIVERSIDADE FEDERAL DE LAVRAS

URL: http://bibtede.ufla.br/tede//tde_busca/arquivo.php?codArquivo=1709

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Streptococcus agalactiae (Lancefield group B; GBS) is an important pathogen to human, bovine and fish causing neonatal sepsis, mastitis and meningo-encephalitis, respectively. The objective of… (more)

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Streptococcus agalactiae (Lancefield group B; GBS) is an important pathogen to human, bovine and fish causing neonatal sepsis, mastitis and meningo-encephalitis, respectively. The objective of this study was to characterize by PFGE S. agalactiae isolated from fish (n=27), bovine (n=9) and human (n=11) and to investigate the virulence of bovine, fish and human strains to Nile tilapia (Oreochromis niloticus). The fish strains were isolated from nine Nile tilapia farms, the bovine strains from nine dairy cow farms and human strains from distinct clinical outcomes. The PFGE types were determined by dendogram analyses and the in vivo virulence was evaluated by experimental infection (using i.p and immersion routes) in Nile tilapia. Among fish strains five different PFGE patterns were observed, including the occurrence of two or three profiles in the same farm. Twenty one of twenty seven strains isolated from fish showed the same genetic pattern (predominant pattern). To bovine and human strains high genetic diversity were observed, but no relations were established to the three host sources analyzed. Regarding the infectivity to Nile tilapia, three bovine strains were able to infect fish (by i.p.route) and two of those strains caused clinical signs of meningoencephalitis. All human strains (n= 5) submitted to experimental infection were able to infect Nile tilapia and clinical disease was induced by one strain (80427). This isolate was also able to infect Nile tilapia by immersion route and induce clinical signs. Eight fish strains of S. agalactiae, belonged to different PFGE types, caused high mortality in Nile tilapia. In conclusion the analyzed strains from the three natural hosts did not show genetic relatedness and, in spite of that, some bovine and human strains were able to infect fish and cause meningoencephalitis. We suggest that these strains share some virulence factors and that genetic linkage is not a prerequisite to S. agalactiae to cross the host-specific barrier.

Streptococcus agalactiae (grupo B de Lancefield; GBS) Ã um importante patÃgeno para seres humanos, bovinos e peixes causando septicemia neonatal, mastite e meningo-encefalite respectivamente. O objetivo desse trabalho foi caracterizar por PFGE S. agalactiae isolados de peixes (n=27), bovinos (n=9) e seres humanos (n=11) e investigar a virulÃncia das amostras de bovino, peixes e humanos para tilÃpias do Nilo(Oreochromis niloticus). As amostras de peixes foram isoladas de nove fazendas produtoras de tilÃpia do Nilo localizadas em seis estados brasileiros, as amostras de bovino foram provenientes de nove fazendas produtoras de leite e as amostras de seres humanos de isolamentos clÃnicos distintos. Os padrÃes de PFGE foram determinados por anÃlise de dendograma e a virulÃncia in vivo foi avaliada por infecÃÃo experimental (usando as vias intraperitoneal e imersÃo) em tilÃpias do Nilo. Entre as amostras de peixes cinco padrÃes diferentes pelo PFGE foram observados, incluindo a ocorrÃncia de dois ou mais padrÃes na mesma fazenda. Vinte uma das vinte…

Advisors/Committee Members: PatrÃcia Gomes Cardoso, Ivy Cristina Menezes de Oliveira, Geraldo Marcio da Costa, Henrique CÃsar Pereira Figueiredo.

Subjects/Keywords: PFGE; peixe; S. agalactiae, fish, human, bovine, PFGE; MEDICINA VETERINARIA; Streptococcus agalactiae

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Pereira, U. d. P. (2008). Genotipagem de Streptococcus agalactiae isolados de seres humanos, bovinos e peixes e seu potencial de virulÃncia para tilÃpia do Nilo. (Thesis). UNIVERSIDADE FEDERAL DE LAVRAS. Retrieved from http://bibtede.ufla.br/tede//tde_busca/arquivo.php?codArquivo=1709

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Pereira, Ulisses de PÃdua. “Genotipagem de Streptococcus agalactiae isolados de seres humanos, bovinos e peixes e seu potencial de virulÃncia para tilÃpia do Nilo.” 2008. Thesis, UNIVERSIDADE FEDERAL DE LAVRAS. Accessed January 16, 2021. http://bibtede.ufla.br/tede//tde_busca/arquivo.php?codArquivo=1709.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Pereira, Ulisses de PÃdua. “Genotipagem de Streptococcus agalactiae isolados de seres humanos, bovinos e peixes e seu potencial de virulÃncia para tilÃpia do Nilo.” 2008. Web. 16 Jan 2021.

Vancouver:

Pereira UdP. Genotipagem de Streptococcus agalactiae isolados de seres humanos, bovinos e peixes e seu potencial de virulÃncia para tilÃpia do Nilo. [Internet] [Thesis]. UNIVERSIDADE FEDERAL DE LAVRAS; 2008. [cited 2021 Jan 16]. Available from: http://bibtede.ufla.br/tede//tde_busca/arquivo.php?codArquivo=1709.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Pereira UdP. Genotipagem de Streptococcus agalactiae isolados de seres humanos, bovinos e peixes e seu potencial de virulÃncia para tilÃpia do Nilo. [Thesis]. UNIVERSIDADE FEDERAL DE LAVRAS; 2008. Available from: http://bibtede.ufla.br/tede//tde_busca/arquivo.php?codArquivo=1709

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

16. Didier Silveira Castellano Filho. PREVALÊNCIA E PERFIL DE SUSCEPTIBILIDADE A DROGAS DE STREPTOCOCUS AGALACTIAE EM PARTURIENTES DO SUS, ATENDIDAS EM UMA MATERNIDADE DE JUIZ DE FORA.

Degree: 2008, Universidade Federal de Juiz de Fora

URL: http://www.bdtd.ufjf.br/tde_busca/arquivo.php?codArquivo=323

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Streptococcus agalactiae or Group B of Lancefield (GBS) can be part of the microbiota of the human beings, colonizing especially the gastrointestinal and the genitourinary… (more)

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Streptococcus agalactiae or Group B of Lancefield (GBS) can be part of the microbiota of the human beings, colonizing especially the gastrointestinal and the genitourinary tract. Its medical importance is associated to the transmission to neonates during the delivery, which can result in severe infection. The prevalence of the GBS among pregnant women ranges from 3% to 41%. International precautions were suggested (detection of the GBS during the prenatal and predelivery prophylaxis therapy) to the prevention of the prenatal disease, which resulted in a decrease in its incidence where they had been adopted. Our work had the aim of isolating and identifying lineages of the GBS in delivering women in a hospital in the city of Juiz de Fora-MG, and esteem its prevalence among these pregnant women. Vaginal and anorectal clinical specimens were collected, with an sterile swab, in 221 pregnant women in labor, between October 2007 and March 2008. The prevalence of GBS colonies was perfomed using classical microbiologic culture, following the specific identification through molecular biology. It was also performed the detection of microorganisms directly in clinical anorectal and vaginal specimens, also through molecular technique. After enriching culture in selective medium and isolating suggestive lineages, the isolated ones were analyzed by their morpho-tinctorial characteristics, absence of catalase production and esculin hydrolysis, and bile sensitiveness. The specific identification was based on the research of the codifying gene for Sip surface protein, conserved in GBS, as well as on the magnification of the specific region of the codifying DNA for the 16S region in the RNA, through molecular biology (PCR). Through the classical microbiological methodology, based on culture, GBS was detected in 21 out of the 221 pregnant women observed (9.5%), and 25 lineages were isolated and identified, taking into account the two isolation sites. Through molecular methodology, GBS was detected in 72 pregnant women observed (32.6%), also taking into consideration both analyzed sites. The susceptibility profile to antimicrobial was also analyzed, and the following drugs have been tested through the disc-diffusion method (Kirby-Bauer): peniciline, ampicillin, cefazolin, ciprofloxacin, vancomycin, erythromycin and clindamycin. The findings showed a reduction in the sensitivity to erythromycin (22.7%) and clindamycin (50%). Bearing in mind that in Brazil prevention strategies to reduce the prevalence of neonatal infection through GBS have not been adopted, and taking into account treatment costs, empirical prophylaxis and the high level of colonization, as observed in this work, one can notice the need for prospective and regional studies on the prevalence and susceptibility profile to GBS drugs, in order to contribute to the preparation of health policies aimed at reducing its vertical transmission

O Streptococcus agalactiae ou Grupo B de Lancefield (EGB) pode fazer parte da microbiota de seres humanos, colonizando principalmente o…

Advisors/Committee Members: Tadeu Coutinho, Cláudio Galuppo Diniz, Marcel de Toledo Vieira, Vania Lúcia da Silva, Maria Auxiliadora Roque de Carvalho.

Subjects/Keywords: CIENCIAS DA SAUDE; Streptococcus agalactiae; Sepse Neonatal; Doença Estreptocócica Perinatal; Streptococcus agalactiae; Neonatal Sepsis; Perinatal Streptococcal disease

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Filho, D. S. C. (2008). PREVALÊNCIA E PERFIL DE SUSCEPTIBILIDADE A DROGAS DE STREPTOCOCUS AGALACTIAE EM PARTURIENTES DO SUS, ATENDIDAS EM UMA MATERNIDADE DE JUIZ DE FORA. (Thesis). Universidade Federal de Juiz de Fora. Retrieved from http://www.bdtd.ufjf.br/tde_busca/arquivo.php?codArquivo=323

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Filho, Didier Silveira Castellano. “PREVALÊNCIA E PERFIL DE SUSCEPTIBILIDADE A DROGAS DE STREPTOCOCUS AGALACTIAE EM PARTURIENTES DO SUS, ATENDIDAS EM UMA MATERNIDADE DE JUIZ DE FORA.” 2008. Thesis, Universidade Federal de Juiz de Fora. Accessed January 16, 2021. http://www.bdtd.ufjf.br/tde_busca/arquivo.php?codArquivo=323.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Filho, Didier Silveira Castellano. “PREVALÊNCIA E PERFIL DE SUSCEPTIBILIDADE A DROGAS DE STREPTOCOCUS AGALACTIAE EM PARTURIENTES DO SUS, ATENDIDAS EM UMA MATERNIDADE DE JUIZ DE FORA.” 2008. Web. 16 Jan 2021.

Vancouver:

Filho DSC. PREVALÊNCIA E PERFIL DE SUSCEPTIBILIDADE A DROGAS DE STREPTOCOCUS AGALACTIAE EM PARTURIENTES DO SUS, ATENDIDAS EM UMA MATERNIDADE DE JUIZ DE FORA. [Internet] [Thesis]. Universidade Federal de Juiz de Fora; 2008. [cited 2021 Jan 16]. Available from: http://www.bdtd.ufjf.br/tde_busca/arquivo.php?codArquivo=323.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Filho DSC. PREVALÊNCIA E PERFIL DE SUSCEPTIBILIDADE A DROGAS DE STREPTOCOCUS AGALACTIAE EM PARTURIENTES DO SUS, ATENDIDAS EM UMA MATERNIDADE DE JUIZ DE FORA. [Thesis]. Universidade Federal de Juiz de Fora; 2008. Available from: http://www.bdtd.ufjf.br/tde_busca/arquivo.php?codArquivo=323

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade do Estado do Rio de Janeiro

17. Camila Serva Pereira. Aderência, invasão e persistência intracelular de estreptococos do grupo B em células epiteliais respiratórias A549.

Degree: Master, 2010, Universidade do Estado do Rio de Janeiro

URL: http://www.bdtd.uerj.br/tde_busca/arquivo.php?codArquivo=2392 ; http://www.bdtd.uerj.br/tde_busca/arquivo.php?codArquivo=2393 ;

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Estreptococos do grupo B (EGB) comumente colonizam adultos saudáveis, sem sintomas, mas sob certas circunstâncias possui a capacidade de invadir tecidos do hospedeiro, evadir da… (more)

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Estreptococos do grupo B (EGB) comumente colonizam adultos saudáveis, sem sintomas, mas sob certas circunstâncias possui a capacidade de invadir tecidos do hospedeiro, evadir da detecção imunológica e causar doenças invasivas graves. Por conseguinte, os EGB continuam sendo uma das principais causas de mortalidade neonatal, pneumonia, sepse e meningite. Contudo, a patogênese desta infecção ainda está pouco elucidada. O sorotipo V é freqüentemente associado à doença invasiva em mulheres adultas não gestantes e o segundo mais prevalente em mulheres grávidas. O principal objetivo deste trabalho foi estudar a aderência, invasão e persistência intracelular de amostras pertencentes ao sorotipo V (88641-vagina/portador e 90186-sangue/paciente) usando as células epiteliais respiratórias A549. As amostras de EGB demonstraram capacidade de aderir e invadir as células epiteliais A549, mas somente a amostra 90186-sangue apresentou maior invasão quando comparada com a de vagina (P <0.001). Ambas as amostras demonstraram persistência intracelular sem replicação no interior das células A549. Apenas o isolado 90186-sangue sobreviveu dentro das células epiteliais até 24h de incubação (P <0,05). A fusão dos lisossomas das células epiteliais com vacúolos contendo bactérias foi observada em células A549 tratadas com Lyso Tracker Grenn DND-26 para todas as amostras testadas. Nossos dados indicam pela primeira vez que as amostras viáveis do sorotipo V permanecem dentro de vacúolos ácidos epiteliais. Curiosamente, a amostra 90186- sangue induziu vacuolização celular e a amostra 88641-vagina promoveu a morte celular após 7h de incubação. Finalmente, nossos resultados aumentam o nosso conhecimento sobre eventos celulares da fagocitose e da patogênese das doenças invasivas promovidas pelos EGB.

Group B Streptococcus (GBS) commonly colonizes healthy asymptomatic adults, yetunder certain circumstances displays the ability to invade host tissues, evade the immune system and cause serious invasive disease. Consequently, GBS remains the major cause of neonatal pneumonia, sepsis and meningitis. However, the pathogenesis of this infection is poorly understood. The serotype V is frequently associated with invasive diseases in non-pregnant adults and the second most prevalent in pregnant women. The aim of this work was to study the adherence; invasion and persistence intracellular of the GBS serotype V (88641-vagina/carriers and 90186-blood/patient) in epithelial cells A549. All GBS strains showed ability to adhere and invade the epithelial A549 cells, but GBS 90186-blood was more invasive than the vagina isolate (P<0,001). Both strains persisted intracellular, but without replicating into the A549 cells. Only 90186-blood strain survived within epithelial cells even after a 24h incubation (P<0,05). Fusion of epithelial lysosomes with bacteria containing phagocytic vacuoles was observed in A549 cells treated with Lysotracker Grenn DND-26 for all strains tested. Our data indicate for the first time that viable strains of serotype V remain within…

Advisors/Committee Members: Ana Cláudia de Paula Rosa Ignacio, Gabriela Santos Jonathan, Angela Corrêa de Freitas-Almeida, Eliane de Oliveira Ferreira, Prescilla Emy Nagao Ferreira.

Subjects/Keywords: Streptococcus agalactiae; Células epiteliais; Vacúolos acídicos; Persistência intracelular; Streptococcus agalactiae; Epithelial cells; Acidic vacuoles; Intracellular persistence; MICROBIOLOGIA

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Pereira, C. S. (2010). Aderência, invasão e persistência intracelular de estreptococos do grupo B em células epiteliais respiratórias A549. (Masters Thesis). Universidade do Estado do Rio de Janeiro. Retrieved from http://www.bdtd.uerj.br/tde_busca/arquivo.php?codArquivo=2392 ; http://www.bdtd.uerj.br/tde_busca/arquivo.php?codArquivo=2393 ;

Chicago Manual of Style (16^th Edition):

Pereira, Camila Serva. “Aderência, invasão e persistência intracelular de estreptococos do grupo B em células epiteliais respiratórias A549.” 2010. Masters Thesis, Universidade do Estado do Rio de Janeiro. Accessed January 16, 2021. http://www.bdtd.uerj.br/tde_busca/arquivo.php?codArquivo=2392 ; http://www.bdtd.uerj.br/tde_busca/arquivo.php?codArquivo=2393 ;.

MLA Handbook (7^th Edition):

Pereira, Camila Serva. “Aderência, invasão e persistência intracelular de estreptococos do grupo B em células epiteliais respiratórias A549.” 2010. Web. 16 Jan 2021.

Vancouver:

Pereira CS. Aderência, invasão e persistência intracelular de estreptococos do grupo B em células epiteliais respiratórias A549. [Internet] [Masters thesis]. Universidade do Estado do Rio de Janeiro; 2010. [cited 2021 Jan 16]. Available from: http://www.bdtd.uerj.br/tde_busca/arquivo.php?codArquivo=2392 ; http://www.bdtd.uerj.br/tde_busca/arquivo.php?codArquivo=2393 ;.

Council of Science Editors:

Pereira CS. Aderência, invasão e persistência intracelular de estreptococos do grupo B em células epiteliais respiratórias A549. [Masters Thesis]. Universidade do Estado do Rio de Janeiro; 2010. Available from: http://www.bdtd.uerj.br/tde_busca/arquivo.php?codArquivo=2392 ; http://www.bdtd.uerj.br/tde_busca/arquivo.php?codArquivo=2393 ;

University of Helsinki

18. Lillqvist, Monica. Juverhälsoproblem orsakade av Streptococcus agalactiae på stora mjölkgårdar med mjölkningsrobot.

Degree: Eläinlääketieteellinen tiedekunta; Helsingfors universitet, Veterinärmedicinska fakulteten, 2011, University of Helsinki

URL: http://hdl.handle.net/10138/26373

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Streptococcus agalactiae –juverinflammation var tidigare ett stort problem i många länder, inklusive Finland. I och med förbättrad mjölkningshygien och antibiotikabehandlingar har bakterien så gott som… (more)

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Streptococcus agalactiae –juverinflammation var tidigare ett stort problem i många länder, inklusive Finland. I och med förbättrad mjölkningshygien och antibiotikabehandlingar har bakterien så gott som eradikerats från mjölkbesättningarna. Nu verkar bakterien i viss mån ha kommit tillbaka till våra stora mjölkgårdar. Avhandlingens experimentella del utfördes på en mjölkgård, med ca 180 mjölkande och tre mjölkningsrobotar, som haft problem med Str. agalactiae. Man hoppades utreda hur stort problemet på gården var samt möjliga smittovägar. Man undersökte också möjligheten att använda mjölkningsrobotens automatiska provtagningsutrustning för provtagning av bakteriella prov. PCRmetoden jämfördes med konventionell odling vid diagnostik av juverinflammationer orsakade av Str. agalactiae. På gården gick man igenom anteckningar samt hälso- och seminkort för att få en bild över situationen. Man gjorde en uppföljning av mjölkningen för tolv kor vid den ena mjölkningsroboten. Man tog 47 stycken kospecifika mjölkprov samt ett prov från mjölktanken. Mjölkprov i tre serier både mjölkade för hand och direkt från mjölkuppsamlaren på mjölkningsroboten togs. Man tog sammanlagt 23 renlighetsprov från mjölkningsroboten, tre från den automatiska provtagningsutrustningen samt två från djurskötarnas händer. Från den automatiska provtagningsutrustningen togs även ett genomsköljningsprov. Av mjölkprov som tidigare tagits på gården hade man hittat Str. agalactiae i ca 17%. I denna studie hittades Str. agalactiae i tre kospecifika mjölkprov, vilket motsvarar en prevalens på ca 2%. Vid uppföljningen av mjölkningarna upptäcktes inget alarmerande, men spenarnas hälsa samt tommjölkningar är något som bör följas upp. Av renlighetsproven hittades Str. agalactiae i ett prov taget från borsthållaren. Svaren från mjölkproven tagna i serier tyder på att den automatiska provtagningsutrustningen inte går att använda till bakteriella prov, eftersom mjölken från en Str. agalactiae –infekterad ko verkar påverka resultatet också hos följande kor. Resultatet är väntat, eftersom mjölkprov alltid skall tas aseptiskt och det går inte med den automatiska provtagningsutrustningen så som den i dagsläget är utvecklad. Från sju av nio mjölkprov, där man hittat Str. agalactiae med PCR-metoden, hittades bakterien också med konventionell odling. Från tankmjölksprovet kunde man inte hitta Str. agalactiae med konventionell odling. PCR-metoden verkar enligt den här studien vara mer känslig att upptäcka Str. agalactiae jämfört med konventionell odling.

Vain tiivistelmä.

Subjects/Keywords: juverinflammation; juverhälsa; mastit; Streptococcus agalactiae; Tuotantoeläinten terveyden- ja sairaanhoito; Produktionsdjurens hälso- och sjukvård; Production Animal Medicine; juverinflammation; juverhälsa; mastit; Streptococcus agalactiae

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lillqvist, M. (2011). Juverhälsoproblem orsakade av Streptococcus agalactiae på stora mjölkgårdar med mjölkningsrobot. (Thesis). University of Helsinki. Retrieved from http://hdl.handle.net/10138/26373

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lillqvist, Monica. “Juverhälsoproblem orsakade av Streptococcus agalactiae på stora mjölkgårdar med mjölkningsrobot.” 2011. Thesis, University of Helsinki. Accessed January 16, 2021. http://hdl.handle.net/10138/26373.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lillqvist, Monica. “Juverhälsoproblem orsakade av Streptococcus agalactiae på stora mjölkgårdar med mjölkningsrobot.” 2011. Web. 16 Jan 2021.

Vancouver:

Lillqvist M. Juverhälsoproblem orsakade av Streptococcus agalactiae på stora mjölkgårdar med mjölkningsrobot. [Internet] [Thesis]. University of Helsinki; 2011. [cited 2021 Jan 16]. Available from: http://hdl.handle.net/10138/26373.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lillqvist M. Juverhälsoproblem orsakade av Streptococcus agalactiae på stora mjölkgårdar med mjölkningsrobot. [Thesis]. University of Helsinki; 2011. Available from: http://hdl.handle.net/10138/26373

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Université de Lorraine

19. Chuzeville, Sarah. Caractérisation des fonctions codées par les éléments intégratifs conjugatifs (ICE) intégrés dans un gène codant un ARNt lysine chez Streptococcus agalactiae : rôle dans le maintien des ICE, l'adaptation et la virulence de l'hôte : Caracterization of the functions encoded by conjugative and integrative elements (ICE) integrated in a gene encoding a tRNA lys in streptococcus agalactiae : role in the maintenance of ICE, adaptation and virulence.

Degree: Docteur es, Procédés biotechnologiques et alimentaires, 2012, Université de Lorraine

URL: http://www.theses.fr/2012LORR0261

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Le transfert horizontal participe à l'évolution rapide des génomes bactériens. Les éléments intégratifs et conjugatifs (ICE) sont des îlots génomiques capables de se transférer par… (more)

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Le transfert horizontal participe à l'évolution rapide des génomes bactériens. Les éléments intégratifs et conjugatifs (ICE) sont des îlots génomiques capables de se transférer par conjugaison vers une bactérie receveuse. Streptococcus agalactiae est une bactérie pathogène opportuniste qui est à l'origine de problèmes sanitaires et économiques majeurs. Des études ont révélé la présence de nombreux ICE chez cette espèce, notamment à l'extrémité 3' d?un gène codant un ARNtLys. La fonctionnalité de l'ICE intégré à ce locus chez la souche 515 de S. agalactiae a été démontrée. Les fonctions véhiculées par ICE_515_tRNALys et pouvant conférer un avantage adaptatif ont été caractérisées et leur transfert vers d'autres espèces a été évalué. Les résultats ont montré que l'ICE confère à S. agalactiae des propriétés d'adhésion à l'hôte et de formation de biofilm et pourrait être impliqué dans l'agrégation cellulaire. Un antigène I/II codé par l'ICE est impliqué dans des phénotypes d'adhésion. De plus, un nouveau facteur co-hémolytique de type CAMP, codé par l'ICE et qui pourrait être impliqué dans la virulence et la survie des souches, a été caractérisé. La fonctionnalité de ces facteurs de virulence chez des espèces bactériennes pathogènes et non pathogènes a été établie. Les travaux ont également révélé la prévalence et la dynamique évolutive des ICE appartenant à la famille d'ICE_515_tRNALys et des fonctions adaptatives codées par ces éléments chez plusieurs espèces de streptocoques. En conclusion, les ICE de la famille d'ICE_515_tRNALys représentent des vecteurs de traits phénotypiques importants pour la virulence et la survie chez les streptocoques

Horizontal gene transfer is a rapid mechanism of evolution. Integrative and conjugative elements (ICEs) are genomic islands which can transfer by conjugation to recipient bacteria. Streptococcus agalactiae is a human and animal opportunistic pathogen that is responsible for major health and economic problems. Studies revealed the presence of numerous ICEs in S. agalactiae, in particular at the 3' end of a tRNALys encoding gene. The functionality of the element present in strain S. agalactiae 515 was demonstrated and was thus chosen as a model for this study. This work focused on the characterization of adaptive and virulence functions encoded by ICE_515_tRNALys and their transfer to other species. Results indicated that this ICE confers adhesion properties to host, increases biofilm formation and may be involved in cell aggregation. A new protein belonging to the antigens I/II family is involved in fibronectin binding and contributes to the biofilm phenotype. In addition, a new co-hemolytic CAMP factor encoded by ICE_515_tRNALys, which could be involved in virulence and bacterial survival, was identified and characterized. These virulence factors are functional in other bacterial species. This work also revealed the prevalence and evolutionary dynamics of ICE belonging to the family of ICE_515_tRNALys and adaptive functions encoded by these elements in several species of…

Advisors/Committee Members: Payot-Lacroix, Sophie (thesis director), Madec, Jean-Yves (thesis director).

Subjects/Keywords: Transfert génétique; Éléments génétiques mobiles; Streptococcus agalactiae; Virulence; Adhésion bactérienne; Gene transfer; Mobile genetic elements; Streptococcus agalactiae; Virulence; Bacterial adhesion; 572.877

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Chuzeville, S. (2012). Caractérisation des fonctions codées par les éléments intégratifs conjugatifs (ICE) intégrés dans un gène codant un ARNt lysine chez Streptococcus agalactiae : rôle dans le maintien des ICE, l'adaptation et la virulence de l'hôte : Caracterization of the functions encoded by conjugative and integrative elements (ICE) integrated in a gene encoding a tRNA lys in streptococcus agalactiae : role in the maintenance of ICE, adaptation and virulence. (Doctoral Dissertation). Université de Lorraine. Retrieved from http://www.theses.fr/2012LORR0261

Chicago Manual of Style (16^th Edition):

Chuzeville, Sarah. “Caractérisation des fonctions codées par les éléments intégratifs conjugatifs (ICE) intégrés dans un gène codant un ARNt lysine chez Streptococcus agalactiae : rôle dans le maintien des ICE, l'adaptation et la virulence de l'hôte : Caracterization of the functions encoded by conjugative and integrative elements (ICE) integrated in a gene encoding a tRNA lys in streptococcus agalactiae : role in the maintenance of ICE, adaptation and virulence.” 2012. Doctoral Dissertation, Université de Lorraine. Accessed January 16, 2021. http://www.theses.fr/2012LORR0261.

MLA Handbook (7^th Edition):

Chuzeville, Sarah. “Caractérisation des fonctions codées par les éléments intégratifs conjugatifs (ICE) intégrés dans un gène codant un ARNt lysine chez Streptococcus agalactiae : rôle dans le maintien des ICE, l'adaptation et la virulence de l'hôte : Caracterization of the functions encoded by conjugative and integrative elements (ICE) integrated in a gene encoding a tRNA lys in streptococcus agalactiae : role in the maintenance of ICE, adaptation and virulence.” 2012. Web. 16 Jan 2021.

Vancouver:

Chuzeville S. Caractérisation des fonctions codées par les éléments intégratifs conjugatifs (ICE) intégrés dans un gène codant un ARNt lysine chez Streptococcus agalactiae : rôle dans le maintien des ICE, l'adaptation et la virulence de l'hôte : Caracterization of the functions encoded by conjugative and integrative elements (ICE) integrated in a gene encoding a tRNA lys in streptococcus agalactiae : role in the maintenance of ICE, adaptation and virulence. [Internet] [Doctoral dissertation]. Université de Lorraine; 2012. [cited 2021 Jan 16]. Available from: http://www.theses.fr/2012LORR0261.

Council of Science Editors:

Chuzeville S. Caractérisation des fonctions codées par les éléments intégratifs conjugatifs (ICE) intégrés dans un gène codant un ARNt lysine chez Streptococcus agalactiae : rôle dans le maintien des ICE, l'adaptation et la virulence de l'hôte : Caracterization of the functions encoded by conjugative and integrative elements (ICE) integrated in a gene encoding a tRNA lys in streptococcus agalactiae : role in the maintenance of ICE, adaptation and virulence. [Doctoral Dissertation]. Université de Lorraine; 2012. Available from: http://www.theses.fr/2012LORR0261

Université de Lorraine

20. Puymège, Aurore. Diversité, dynamique et mobilité des éléments intégratifs conjugatifs (ICE) de Streptococcus agalactiae intégrés dans l'extrémité 3' du gène codant un ARNt Lysine : Diversity, dynamic and mobility of "Integrative Conjugative Elements" (ICEs) of Streptococcus agalactiae integrated into the 3' end of tRNA lysine gene.

Degree: Docteur es, Procédés biotechnologiques et alimentaires, 2013, Université de Lorraine

URL: http://www.theses.fr/2013LORR0147

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Les éléments intégratifs conjugatifs (ICE) et les éléments en dérivant jouent un rôle important dans le transfert horizontal de gènes chez les bactéries. Les ICE… (more)

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Les éléments intégratifs conjugatifs (ICE) et les éléments en dérivant jouent un rôle important dans le transfert horizontal de gènes chez les bactéries. Les ICE s'excisent par recombinaison site-spécifique sous forme circulaire, se transfèrent par conjugaison et s'intègrent dans un réplicon de la cellule réceptrice. Streptococcus agalactiae est une bactérie pathogène opportuniste responsable d'infections néonatales sévères chez l'Homme et d'infections chez les animaux (bovins, poissons, ...). Une analyse in silico antérieure de 8 génomes séquencés de Streptococcus agalactiae avait permis d'identifier plusieurs éléments intégrés dans l'extrémité 3' d'un gène codant un ARNtLys CTT dont 4 ICE putatifs. Cette étude élargie à 246 génomes a confirmé la prévalence et la diversité des éléments intégrés dans ce locus (présence d'ICE, éléments mobilisables en trans ou en cis, éléments composites, ... chez 98 % des souches). Une nouvelle famille d'éléments mobilisables putatifs s'intégrant dans l'oriT d'ICE a été caractérisée. L'étude fonctionnelle de 5 ICE a montré que 4 s'excisent du chromosome mais que seuls ICE_FSL S3-026_tRNALys et ICE_515_tRNALys se transfèrent par conjugaison au sein de l'espèce et vers S. pyogenes pour l'un des 2. Des éléments composites ont été obtenus par transfert d'ICE_515_tRNALys vers une souche possédant déjà un élément intégré dans ce locus. Un de ces éléments composites est capable de s'exciser et de se transférer par conjugaison conduisant à une mobilisation en cis de l'élément résident. En conclusion, les ICE et les éléments mobilisables (en cis ou en trans) sont très répandus chez S. agalactiae et contribuent à la plasticité génomique chez cette espèce

Integrative and Conjugative Elements (ICEs) and related elements are widespread in bacteria and play a key role in horizontal gene transfer. ICEs excise by site-specific recombination as a circular intermediate, promote their own transfer by conjugation and then integrate into a replicon of the recipient cell. Streptococcus agalactiae is an opportunistic pathogen that causes severe human invasive neonatal infections as well as infections in animals (bovine, fish...). Previous in silico analysis of eight sequenced genomes of S. agalactiae identified in each genome a different element integrated in the tRNALys CTT gene with four putative ICEs. This study, carried on 246 other genomes of S. agalactiae, confirmed the prevalence and diversity of elements integrated in this locus with 98% of the strains carrying an element (ICE, trans or cis mobilizable elements composite elements...). A novel family of putative mobilisable elements which can integrate in the oriT of ICE has been characterized. Functional analysis of 5 ICEs demonstrated that four can excise of the chromosome but that only ICE_FSLS3-026_tRNALys and ICE_515_tRNALys can transfer by conjugation inside the species or to S. pyogenes for one of them. Composite elements have been obtained after transfer of ICE_515_tRNALys to a recipient strain already carrying an element integrated in…

Advisors/Committee Members: Payot-Lacroix, Sophie (thesis director), Guedon, Gérard (thesis director).

Subjects/Keywords: Streptococcus agalactiae; Diversité; Prévalence; ICE; Transfert conjugatif et mobilité; Streptococcus agalactiae; Diversity; Prevalence; ICE; Conjugative transfer and mobility; 572.869

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Puymège, A. (2013). Diversité, dynamique et mobilité des éléments intégratifs conjugatifs (ICE) de Streptococcus agalactiae intégrés dans l'extrémité 3' du gène codant un ARNt Lysine : Diversity, dynamic and mobility of "Integrative Conjugative Elements" (ICEs) of Streptococcus agalactiae integrated into the 3' end of tRNA lysine gene. (Doctoral Dissertation). Université de Lorraine. Retrieved from http://www.theses.fr/2013LORR0147

Chicago Manual of Style (16^th Edition):

Puymège, Aurore. “Diversité, dynamique et mobilité des éléments intégratifs conjugatifs (ICE) de Streptococcus agalactiae intégrés dans l'extrémité 3' du gène codant un ARNt Lysine : Diversity, dynamic and mobility of "Integrative Conjugative Elements" (ICEs) of Streptococcus agalactiae integrated into the 3' end of tRNA lysine gene.” 2013. Doctoral Dissertation, Université de Lorraine. Accessed January 16, 2021. http://www.theses.fr/2013LORR0147.

MLA Handbook (7^th Edition):

Puymège, Aurore. “Diversité, dynamique et mobilité des éléments intégratifs conjugatifs (ICE) de Streptococcus agalactiae intégrés dans l'extrémité 3' du gène codant un ARNt Lysine : Diversity, dynamic and mobility of "Integrative Conjugative Elements" (ICEs) of Streptococcus agalactiae integrated into the 3' end of tRNA lysine gene.” 2013. Web. 16 Jan 2021.

Vancouver:

Puymège A. Diversité, dynamique et mobilité des éléments intégratifs conjugatifs (ICE) de Streptococcus agalactiae intégrés dans l'extrémité 3' du gène codant un ARNt Lysine : Diversity, dynamic and mobility of "Integrative Conjugative Elements" (ICEs) of Streptococcus agalactiae integrated into the 3' end of tRNA lysine gene. [Internet] [Doctoral dissertation]. Université de Lorraine; 2013. [cited 2021 Jan 16]. Available from: http://www.theses.fr/2013LORR0147.

Council of Science Editors:

Puymège A. Diversité, dynamique et mobilité des éléments intégratifs conjugatifs (ICE) de Streptococcus agalactiae intégrés dans l'extrémité 3' du gène codant un ARNt Lysine : Diversity, dynamic and mobility of "Integrative Conjugative Elements" (ICEs) of Streptococcus agalactiae integrated into the 3' end of tRNA lysine gene. [Doctoral Dissertation]. Université de Lorraine; 2013. Available from: http://www.theses.fr/2013LORR0147

21. Zorgani, Mohamed Amine. Caractérisation des ARN régulateurs chez Streptococcus agalactiae : Characterization of regulatory RNAs in Streptococcus agalactiae.

Degree: Docteur es, Sciences de la Vie et de la Santé, 2016, Université François-Rabelais de Tours

URL: http://www.theses.fr/2016TOUR3309

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Streptococcus agalactiae, appelé aussi Group B Streptococcus (GBS), est une bactérie commensale du tractus digestif et génital de diverses espèces animales dont l’espèce humaine. Elle… (more)

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Streptococcus agalactiae, appelé aussi Group B Streptococcus (GBS), est une bactérie commensale du tractus digestif et génital de diverses espèces animales dont l’espèce humaine. Elle représente la première cause d’infections néonatales et est aussi un pathogène émergent chez l’adulte immunodéprimé. L’objectif de ma thèse est la caractérisation fonctionnelle et mécanistique des ARNrég. J’ai étudié plus particulièrement l’ARNrég CetR (pour «cell-envelope-targeting RNA»). Il module la résistance au peptide antimicrobiens (PAM) et la virulence à travers la régulation post-transcriptionnelle de l’ARNm dltD codant une protéine de biosynthèse de l'acide D-alanyl-lipotéichoïque. La délétion de cetR induit des changements dans la morphologie cellulaire, une diminution de la formation du biofilm et de la résistance aux PAM. Une zone d’interaction, CetRdltD, de 27 nucléotides a été prédite in silico. Des mutations compensatoires chez GBS montrent que CetR interagit directement avec l’ARNm dltD et que la perturbation de la zone d’appariement est suffisante pour observer les phénotypes associés à CetR. La quantification des niveaux d’ARNm et de la protéine DltD nous a permis de montrer que CetR active la traduction de dltD et que la perturbation du duplex CetR-dltD induit une diminution spectaculaire de la protéine DltD. De plus, en utilisant un modèle murin d’infection et en quantifiant la survie des bactéries dans les macrophages, nous avons montré que CetR et DltD sont cruciaux pour la virulence de GBS. Enfin, une approche protéomique globale nous a permis de montrer que CetR joue un rôle important dans l’expression des protéines dites « moonlighting » et de certains facteurs de virulence potentiels. Cet ARNrég peut jouer un rôle important dans la capacité de S. agalactiae à s'établir dans son biotope et à exprimer ses facteurs de virulence. Enfin, les résultats de ces recherches sont des prérequis au développement de stratégies permettant de réduire le risque des infections néonatales dues à S. agalactiae.

The opportunistic pathogen group B Streptococcus (GBS) is the leading cause of neonatal infections. The aim of this work is the characterization of a 680 nt-long regulatory RNA, CetR (cell-envelope-targeting RNA). It modulates antimicrobial peptides (AMPs) resistance and virulence through posttranscriptional regulation of dltD mRNA which encodes a D-alanyl-lipoteichoic acid biosynthesis protein. Deletion of cetR leads to cell morphology changes, reduced biofilm formation and AMPs resistance. A 27 nt-long CetR-dltD interacting region is predicted in silico. Compensatory base pair exchanges in GBS demonstrate that CetR interacts directly with dltD mRNA and that disruption of this RNA pairing is sufficient to observe the CetR-associated phenotypes. By quantifying both mRNA and protein, we demonstrate that CetR enhances dltD translation and disruption of the CetR/dltD mRNA interaction results in a dramatic decrease in DltD protein. Moreover, using an infection murine model and quantifying bacterial survival in macrophages,…

Advisors/Committee Members: Lartigue, Marie-Frédérique (thesis director), Quentin, Roland (thesis director).

Subjects/Keywords: ARN régulateurs; Streptococcus agalactiae; Opéron dlt; Virulence; Protéome global; Regulatory RNA; Streptococcus agalactiae; Dlt operon; Virulence; Global proteome

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Zorgani, M. A. (2016). Caractérisation des ARN régulateurs chez Streptococcus agalactiae : Characterization of regulatory RNAs in Streptococcus agalactiae. (Doctoral Dissertation). Université François-Rabelais de Tours. Retrieved from http://www.theses.fr/2016TOUR3309

Chicago Manual of Style (16^th Edition):

Zorgani, Mohamed Amine. “Caractérisation des ARN régulateurs chez Streptococcus agalactiae : Characterization of regulatory RNAs in Streptococcus agalactiae.” 2016. Doctoral Dissertation, Université François-Rabelais de Tours. Accessed January 16, 2021. http://www.theses.fr/2016TOUR3309.

MLA Handbook (7^th Edition):

Zorgani, Mohamed Amine. “Caractérisation des ARN régulateurs chez Streptococcus agalactiae : Characterization of regulatory RNAs in Streptococcus agalactiae.” 2016. Web. 16 Jan 2021.

Vancouver:

Zorgani MA. Caractérisation des ARN régulateurs chez Streptococcus agalactiae : Characterization of regulatory RNAs in Streptococcus agalactiae. [Internet] [Doctoral dissertation]. Université François-Rabelais de Tours; 2016. [cited 2021 Jan 16]. Available from: http://www.theses.fr/2016TOUR3309.

Council of Science Editors:

Zorgani MA. Caractérisation des ARN régulateurs chez Streptococcus agalactiae : Characterization of regulatory RNAs in Streptococcus agalactiae. [Doctoral Dissertation]. Université François-Rabelais de Tours; 2016. Available from: http://www.theses.fr/2016TOUR3309

22. Moulin, Pauline. Caractérisation du transporteur de zinc Adc/Lmb de Streptococcus agalactiae : Characterization of the ADC/LMB zinc transporter of Streptococcus agalactiae.

Degree: Docteur es, Sciences de la Vie et de la Santé, 2017, Université François-Rabelais de Tours

URL: http://www.theses.fr/2017TOUR3308

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Dans cette étude, le transporteur ABC de zinc de Streptococcus agalactiae, première cause d’infections materno-foetale en France, a été caractérisé. Nous avons montré que ce… (more)

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Dans cette étude, le transporteur ABC de zinc de Streptococcus agalactiae, première cause d’infections materno-foetale en France, a été caractérisé. Nous avons montré que ce transporteur se compose, du complexe perméase-ATPase AdcCB, associé à trois protéines membranaires Lmb, AdcA et AdcAII redondantes dans la fixation de zinc. Ce transporteur comporte également deux protéines Sht et ShtII, retrouvées au niveau de la paroi, et nécessaires aux protéines Lmb et AdcAII pour la capture de zinc. L’absence d’un transporteur fonctionnel, par la triple délétion des gènes lmb, adcA et adcAII ou du complexe adcCB, a révélé une inhibition de la croissance et une perturbation de la division de la bactérie lorsqu’elle se trouve dans un environnement carencé en zinc. De plus, nous avons montré que ce transporteur de zinc participe à la survie de la bactérie en milieux biologiques humains, comme le liquide amniotique ou le LCR, où la bactérie est retrouvée lors d’infections, suggérant l’importance du transporteur lors du processus infectieux. Ces résultats ont mis en évidence, pour la première fois, que le zinc assure des fonctions biologiques vitales pour S. agalactiae et que, dans des conditions de forte carence en zinc, le transporteur Adc/Lmb représente le principal système d’acquisition de zinc de la bactérie.

In this study, the zinc-ABC transporter of Streptococcus agalactiae, the first cause of materno-foetal infections in France, was characterized. We showed that this transporter is composed of an AdcCB permease-ATPase complex in association with three membrane-associated proteins Lmb, AdcA and AdcAII, which are redundant in zinc-binding. This transporter also possesses two proteins Sht and ShtII, which are associated to the cell wall, and that are necessary for the Lmb and AdcAII proteins for zinc capture. The absence of a functional transporter, by the triple deletion of the lmb, adcA and adcAII genes or the adcCB complex, revealed a growth inhibition and a disruption of the division of the bacterium when it is in a zinc-restricted environment. Furthermore, we showed that the zinc-ABC transporter contributes to the survival of the bacterium in human biological fluids, as the amniotic fluid or the cerebrospinal fluid, where the bacterium is found during infections, suggesting the importance of the transporter during the infectious process. These results hightlighted, for the first time, that zinc has biologically vital functions in S. agalactiae and that, under high zinc deficiency conditions, the Adc/Lmb transporter is the main zinc acquisition system of the bacterium.

Advisors/Committee Members: Mereghetti, Laurent (thesis director).

Subjects/Keywords: Streptococcus agalactiae; Transporteur ABC; Homéostasie du zinc; Protéine fixatrice de métaux; Streptococcus agalactiae; ABC transporter; Zinc homeostasis; Metal-binding protein

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Moulin, P. (2017). Caractérisation du transporteur de zinc Adc/Lmb de Streptococcus agalactiae : Characterization of the ADC/LMB zinc transporter of Streptococcus agalactiae. (Doctoral Dissertation). Université François-Rabelais de Tours. Retrieved from http://www.theses.fr/2017TOUR3308

Chicago Manual of Style (16^th Edition):

Moulin, Pauline. “Caractérisation du transporteur de zinc Adc/Lmb de Streptococcus agalactiae : Characterization of the ADC/LMB zinc transporter of Streptococcus agalactiae.” 2017. Doctoral Dissertation, Université François-Rabelais de Tours. Accessed January 16, 2021. http://www.theses.fr/2017TOUR3308.

MLA Handbook (7^th Edition):

Moulin, Pauline. “Caractérisation du transporteur de zinc Adc/Lmb de Streptococcus agalactiae : Characterization of the ADC/LMB zinc transporter of Streptococcus agalactiae.” 2017. Web. 16 Jan 2021.

Vancouver:

Moulin P. Caractérisation du transporteur de zinc Adc/Lmb de Streptococcus agalactiae : Characterization of the ADC/LMB zinc transporter of Streptococcus agalactiae. [Internet] [Doctoral dissertation]. Université François-Rabelais de Tours; 2017. [cited 2021 Jan 16]. Available from: http://www.theses.fr/2017TOUR3308.

Council of Science Editors:

Moulin P. Caractérisation du transporteur de zinc Adc/Lmb de Streptococcus agalactiae : Characterization of the ADC/LMB zinc transporter of Streptococcus agalactiae. [Doctoral Dissertation]. Université François-Rabelais de Tours; 2017. Available from: http://www.theses.fr/2017TOUR3308

23. Salloum, Mazen. Les infections à "Streptococus agalactiae" chez l'adulte : emergence et impact de la lysogénie. : Infections due to streptococcus agalactiae in adluts : emergence and impact of lysogeny.

Degree: Docteur es, Sciences de la Vie et de la Santé, 2010, Université François-Rabelais de Tours

URL: http://www.theses.fr/2010TOUR3144

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Streptococcus Agalactiae est depuis les années 1990, responsable d'infections invasives émergentes chez l'adulte. Nous montrons queles souches responsables de ces infections appartiennent majoritairement aux sérotypes… (more)

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Streptococcus Agalactiae est depuis les années 1990, responsable d'infections invasives émergentes chez l'adulte. Nous montrons queles souches responsables de ces infections appartiennent majoritairement aux sérotypes V et Ia et aux deux clones phylogénétiquement éloignés, CC1 et CC23. L'étude du contenu prophagique montre une lysogénie fréquente suggérant l'importance de la lysogénie dans la spécialisation de ces souches particulièrement aptes à infecter l'adulte. Dans un deuxième temps, nous avons isolé sept phages tempérés de souches associées à des infections cutanées et ostéo-articulaires. Ces phages appartiennent à la famille des SIPHOVIRIDAE. L’analyse par restriction enzymatique de l’ADN phagique et l’amplification par PCR de fragments d’ADN prophagique a montré la diversité de ces phages et leurdifférence des phages isolés de souches associées aux infections materno-foetales. Les phagesisolés de souches lysogènes de CC1 ont présenté un spectre lytique étendu aux souches de tous les clones intra-species.

Streptococcus agalactiae has emerged since 1990 in infections in nonpregnant adults, We showed that the strains isolated from adult infections were mainly of serotypes V and Ia., and mainly belonged to the two phylogenetically distant clones, CC1 and CC23. The prophagic content study showed a frequent lysogeny, suggesting a role of lysegeny in the specialization of these strains able to infect adult. Also, we isolated seven phages from strains associated with cutaneous and osteoarticular infections in adult. Ces phages classified among SIPHOVIRIDAE. Restriction analysis of phagic DNA and PCR for prophagic DNA showed genetiacally diverse phages, distinct from the phages isolated from strains responsible for materno-foetal infections. Phages isolated from lysogenic strains of CC1 had a wide lytic spectrum and were able to lyse strains belonging to all clones intra-species.

Advisors/Committee Members: Quentin, Roland (thesis director).

Subjects/Keywords: Streptococcus agalactiae; Bactériophage; Lysogénie; Streptococcus agalactiae; Bacteriophage; Lysogénie

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Salloum, M. (2010). Les infections à "Streptococus agalactiae" chez l'adulte : emergence et impact de la lysogénie. : Infections due to streptococcus agalactiae in adluts : emergence and impact of lysogeny. (Doctoral Dissertation). Université François-Rabelais de Tours. Retrieved from http://www.theses.fr/2010TOUR3144

Chicago Manual of Style (16^th Edition):

Salloum, Mazen. “Les infections à "Streptococus agalactiae" chez l'adulte : emergence et impact de la lysogénie. : Infections due to streptococcus agalactiae in adluts : emergence and impact of lysogeny.” 2010. Doctoral Dissertation, Université François-Rabelais de Tours. Accessed January 16, 2021. http://www.theses.fr/2010TOUR3144.

MLA Handbook (7^th Edition):

Salloum, Mazen. “Les infections à "Streptococus agalactiae" chez l'adulte : emergence et impact de la lysogénie. : Infections due to streptococcus agalactiae in adluts : emergence and impact of lysogeny.” 2010. Web. 16 Jan 2021.

Vancouver:

Salloum M. Les infections à "Streptococus agalactiae" chez l'adulte : emergence et impact de la lysogénie. : Infections due to streptococcus agalactiae in adluts : emergence and impact of lysogeny. [Internet] [Doctoral dissertation]. Université François-Rabelais de Tours; 2010. [cited 2021 Jan 16]. Available from: http://www.theses.fr/2010TOUR3144.

Council of Science Editors:

Salloum M. Les infections à "Streptococus agalactiae" chez l'adulte : emergence et impact de la lysogénie. : Infections due to streptococcus agalactiae in adluts : emergence and impact of lysogeny. [Doctoral Dissertation]. Université François-Rabelais de Tours; 2010. Available from: http://www.theses.fr/2010TOUR3144

24. Belami Cassia da Silva. Isolation and Identification of Staphylococcus aureus and Streptococcus agalactiae of the milk bovine.

Degree: 2007, UNIVERSIDADE FEDERAL DE LAVRAS

URL: http://bibtede.ufla.br/tede//tde_busca/arquivo.php?codArquivo=635

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The objective of this work was to evaluate the microbiologic and physicochemical quality of raw milk of rural estates of the micro-region of Lavras-MG. Milk… (more)

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The objective of this work was to evaluate the microbiologic and physicochemical quality of raw milk of rural estates of the micro-region of Lavras-MG. Milk samples coming from 25 herds collected from cans were analyzed, microbiological analysis such as total count of mesophyll aerobes, Staphylococcus aureus and Streptococcus agalactiae, physicochemical analyses: density, titrable acidity, fat, protein, total and non-fat solids, sodium chloride, lactose and count of somatic cells. There was no correlation between the somatic cell count and the other physicochemical variables. For the variables density, acidity, fat, total and non-fat solids, the results found were statistically equal in the different seasons analyzed. The contents of chlorites, crude and true protein was statistically different. As regards lactose, its contents were considered low for healthy cows. Staphylococcus aureus was the most isolated contagious mastitis agent due to the ease of dissemination during all the milking process, differently from Streptococcus agalactiae which is a strictly contagious pathogen. The count of mesophyll aerobes proved to be above the one allowed by the legislation at 90% of the farms. Concerning the count of somatic cells, distinct behaviors in the different seasons of the year were obtained. It follows that milk quality can be significantly improved by means of technical assistance to the farmers and adequate sanitization of milking can and utensils, enabling improved environment for milking, cooling milk on the farm and promoting its fast transportation.

Com o objetivo de avaliar a qualidade microbiolÃgica e fÃsico-quÃmica do leite cru de propriedades rurais da micro regiÃo de Lavras-MG, foram analisadas amostras de leite provenientes de 25 rebanhos, coletadas de latÃes, realizou-se anÃlises microbiolÃgicas como Contagem Total de AerÃbios MesÃfilos, Staphylococcus aureus e Streptococcus agalactiae, anÃlises fÃsico-quÃmicas: densidade, acidez titulÃvel, gordura, proteÃna, sÃlidos totais e desengordurados, cloreto de sÃdio, lactose e contagem de cÃlulas somÃticas. NÃo houve correlaÃÃo entre a contagem de cÃlulas somÃticas e as demais variÃveis fÃsico-quÃmica. Para as variÃveis densidade, acidez, gordura, sÃlidos totais e desengordurados os resultados encontrados foram estatisticamente iguais nas diferentes estaÃÃes analisadas. Os teores de cloretos, proteÃna bruta e verdadeira foram estatisticamente diferentes. Quanto Ã lactose seus teores foram considerados baixo para vacas sadias. O Staphylococcus aureus foi o agente contagioso da mastite mais isolado devido facilidade de disseminaÃÃo durante todo o processo de ordenha, diferentemente do Streptococcus agalactiae que Ã um patÃgeno estritamente contagioso. A contagem de AerÃbios MesÃfilos apresentou-se acima do permitido pela legislaÃÃo em 90% das propriedades. Quanto Ã contagem de cÃlulas somÃticas obtiveram-se comportamentos distintos nas diferentes Ãpocas do ano. Conclui-se que a qualidade do leite pode ser significativamente melhorada por meio de assistÃncia…

Advisors/Committee Members: Roberta Hilsdorf Piccoli, Alexandre Tourino MendonÃa, Sandra Maria Pinto, Luiz Ronaldo de Abreu.

Subjects/Keywords: Leite cru; streptococcus agalactiae; Streptococcus; MICROBIOLOGIA; Staphylococcus; mastitis; mastite; milk bovine; staphylococcus aureus

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Silva, B. C. d. (2007). Isolation and Identification of Staphylococcus aureus and Streptococcus agalactiae of the milk bovine. (Thesis). UNIVERSIDADE FEDERAL DE LAVRAS. Retrieved from http://bibtede.ufla.br/tede//tde_busca/arquivo.php?codArquivo=635

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Silva, Belami Cassia da. “Isolation and Identification of Staphylococcus aureus and Streptococcus agalactiae of the milk bovine.” 2007. Thesis, UNIVERSIDADE FEDERAL DE LAVRAS. Accessed January 16, 2021. http://bibtede.ufla.br/tede//tde_busca/arquivo.php?codArquivo=635.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Silva, Belami Cassia da. “Isolation and Identification of Staphylococcus aureus and Streptococcus agalactiae of the milk bovine.” 2007. Web. 16 Jan 2021.

Vancouver:

Silva BCd. Isolation and Identification of Staphylococcus aureus and Streptococcus agalactiae of the milk bovine. [Internet] [Thesis]. UNIVERSIDADE FEDERAL DE LAVRAS; 2007. [cited 2021 Jan 16]. Available from: http://bibtede.ufla.br/tede//tde_busca/arquivo.php?codArquivo=635.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Silva BCd. Isolation and Identification of Staphylococcus aureus and Streptococcus agalactiae of the milk bovine. [Thesis]. UNIVERSIDADE FEDERAL DE LAVRAS; 2007. Available from: http://bibtede.ufla.br/tede//tde_busca/arquivo.php?codArquivo=635

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

25. Cleia Maria Monteiro da Cunha. Perfil de suscetibilidade e detecção de marcadores genéticos de resistência em Streptococcus Agalactiae isolados de amostras animais e humanas.

Degree: 2008, Universidade Federal Rural do Rio de Janeiro

URL: http://bdtd.ufrrj.br//tde_busca/arquivo.php?codArquivo=843

► Os Streptococcus agalactiae, também designados como estreptococos do grupo B (EGB), são microrganismos comensais adaptados para fazer a colonização assintomática do tubo digestivo, e do… (more)

▼ Os Streptococcus agalactiae, também designados como estreptococos do grupo B (EGB), são microrganismos comensais adaptados para fazer a colonização assintomática do tubo digestivo, e do trato geniturinário de mamíferos. Inicialmente, reconhecida como um dos mais importantes agentes etiológicos da mastite bovina, esta espécie foi também implicada como uma das principais causas de infecções invasivas em recém-nascidos humanos. As razões para a rápida e consistente evolução do EGB como importante agente causal de infecções neonatais ainda não foram completamente elucidadas, uma vez que as subpopulações de EGB nos isolados de humanos e bovinos são independentes e distintas com base no reconhecimento da diversidade de suas características fisiológicas. Com o objetivo de contribuir para a caracterização das duas subpopulações de S. agalactiae que coexistem no Estado do Rio de Janeiro, este estudo avaliou aspectos da diversidade genética e fenotípica de dois grupos de EGB regionais, sendo o primeiro composto por 50 isolados obtidos a partir de espécimes clínicos humanos, e o segundo constituído por 36 isolados a partir de leite de vacas leiteiras com indícios de mastite clínica ou subclínica. A caracterização fenotípica dos isolados foi baseada em testes sorológicos e fisiológicos, testes de suscetibilidade antimicrobiana realizados com técnica padronizada para utilização de disco de difusão e pelo método de microdiluição. O aspecto genético foi avaliado pela aplicação de PCR para detecção de genes associados à resistência à tetraciclina. Os testes fisiológicos demonstraram que a capacidade de promover β-hemólise era uma característica partilhada por cerca de 28% dos isolados a partir do material de natureza bovina, mas que manifestava-se em todos os isolados de origem humana. Os isolados de EGB bovinos também mostraram um perfil diferente quanto à sensibilidade à bacitracina, uma vez que apenas 33% delas se revelaram suscetíveis a esse antibiótico contra 100% de sensibilidade para os isolados de origem humana. O percentual de 100% de sensibilidade à penicilina demonstrado por todos os isolados analisados neste estudo, também corrobora a importância do uso desse antibiótico como procedimento geral na terapia de infecções por EGB. De uma forma geral, neste estudo foi observado que os isolados originados de material bovino demonstraram percentuais de resistência ao conjunto de antibióticos analisados (cefoxitina, eritromicina, clindamicina, sulfametoxazol, azitromicina e ciprofloxacina) superiores aos observados em isolados de material humano. Foi também observado que 13,9% dos isolados de EGB animais examinados expressaram o fenótipo cMLSB e 2,8% o fenótipo M. O fenótipo M foi o único parâmetro MLSB expresso entre os isolados de S. agalactiae humanos, com um percentual de 6%. Quanto à presença de genes de resistência a tetraciclina entre as subpopulações de EGB, detectou-se percentuais de 13,8% (5/36) e 14% (7/50) para tet(M), e 30,5% (11/36) e 10% (5/50) para tet(O), respectivamente, nos isolados bovinos e humanos… Advisors/Committee Members: Miliane Moreira Soares de Souza.

Subjects/Keywords: Streptococcus agalactiae; caracterização fenotípica; gene tet; MICROBIOLOGIA; fenotipic characterization

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Cunha, C. M. M. d. (2008). Perfil de suscetibilidade e detecção de marcadores genéticos de resistência em Streptococcus Agalactiae isolados de amostras animais e humanas. (Thesis). Universidade Federal Rural do Rio de Janeiro. Retrieved from http://bdtd.ufrrj.br//tde_busca/arquivo.php?codArquivo=843

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Cunha, Cleia Maria Monteiro da. “Perfil de suscetibilidade e detecção de marcadores genéticos de resistência em Streptococcus Agalactiae isolados de amostras animais e humanas.” 2008. Thesis, Universidade Federal Rural do Rio de Janeiro. Accessed January 16, 2021. http://bdtd.ufrrj.br//tde_busca/arquivo.php?codArquivo=843.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Cunha, Cleia Maria Monteiro da. “Perfil de suscetibilidade e detecção de marcadores genéticos de resistência em Streptococcus Agalactiae isolados de amostras animais e humanas.” 2008. Web. 16 Jan 2021.

Vancouver:

Cunha CMMd. Perfil de suscetibilidade e detecção de marcadores genéticos de resistência em Streptococcus Agalactiae isolados de amostras animais e humanas. [Internet] [Thesis]. Universidade Federal Rural do Rio de Janeiro; 2008. [cited 2021 Jan 16]. Available from: http://bdtd.ufrrj.br//tde_busca/arquivo.php?codArquivo=843.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Cunha CMMd. Perfil de suscetibilidade e detecção de marcadores genéticos de resistência em Streptococcus Agalactiae isolados de amostras animais e humanas. [Thesis]. Universidade Federal Rural do Rio de Janeiro; 2008. Available from: http://bdtd.ufrrj.br//tde_busca/arquivo.php?codArquivo=843

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Hong Kong

26. 吳依婷. Multilocus sequence typing for streptococcus agalactiae.

Degree: 2011, University of Hong Kong

URL: http://hdl.handle.net/10722/144026

Subjects/Keywords: Streptococcus agalactiae - Molecular aspects.

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

吳依婷.. (2011). Multilocus sequence typing for streptococcus agalactiae. (Thesis). University of Hong Kong. Retrieved from http://hdl.handle.net/10722/144026

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

吳依婷.. “Multilocus sequence typing for streptococcus agalactiae.” 2011. Thesis, University of Hong Kong. Accessed January 16, 2021. http://hdl.handle.net/10722/144026.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

吳依婷.. “Multilocus sequence typing for streptococcus agalactiae.” 2011. Web. 16 Jan 2021.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Vancouver:

吳依婷.. Multilocus sequence typing for streptococcus agalactiae. [Internet] [Thesis]. University of Hong Kong; 2011. [cited 2021 Jan 16]. Available from: http://hdl.handle.net/10722/144026.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

吳依婷.. Multilocus sequence typing for streptococcus agalactiae. [Thesis]. University of Hong Kong; 2011. Available from: http://hdl.handle.net/10722/144026

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

27. Irina Lermontov Borger. Estudo da Colonização por Streptococcus Agalactiae em Gestantes atendidas na Maternidade Escola da Universidade Federal do Rio de Janeiro.

Degree: 2005, Universidade Federal Fluminense

URL: http://www.bdtd.ndc.uff.br/tde_busca/arquivo.php?codArquivo=310

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This study evaluated the vaginal/anal colonization by Streptococcus agalactiae among 167 pregnant women between 32nd and 41st weeks of gestation, attended in the prenatal ambulatory… (more)

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This study evaluated the vaginal/anal colonization by Streptococcus agalactiae among 167 pregnant women between 32nd and 41st weeks of gestation, attended in the prenatal ambulatory of the School Maternity of Rio de Janeiro University (UFRJ), during the period from february 2003 to february 2004. The frequency of colonization was of 19,2%, without significant differences when variants like age, number of gestations, premature delivery in previous pregnancies, number of abortions and the presence or absence of diabetes (p>0,05) were compared. All 32 strains isolated were susceptible to penicillin, cefotaxime, ofloxacin, chloramphenicol, vancomycin and meropenem. Resistance to penicillin of two strains, as detected by disk diffusion susceptibility test, was not confirmed by dilution antimicrobial susceptibility tests. The concomitant resistance to clindamycin and erythromycin was verified in 2 strains; these presented the constitutive MLSB phenotype. The isolate resistant only to erythromycin presented the M phenotype. The relatively high incidence (19,2%) of colonization by S. agalactiae among the pregnant women evaluated and the recovery of antimicrobial resistant strains, specialy those recommended in cases of penicillin allergy, emphasize the importance, for a correct prevention of neonatal infections, to detect colonization at the end of pregnancy and to evaluate the antimicrobial susceptibility of S. agalacti

Este estudo avaliou a colonização vaginal/anal por Streptococcus agalactiae em 167 grávidas entre a 32a e 41a semanas de gestação, atendidas no ambulatório prénatal da Maternidade Escola da Universidade Federal do Rio de Janeiro (UFRJ), no período de fevereiro de 2003 a fevereiro de 2004. A freqüência de colonização foi de 19,2%, não havendo diferenças significativas quando comparadas variáveis como faixa etária, número de gestações, hipertensão, doenças auto-imunes, prematuridade em gestações anteriores, número de abortos e a presença ou não de diabetes (p>0.05). Todas as 32 amostras isoladas foram sensíveis à penicilina, cefotaxima, ofloxacina, cloranfenicol, vancomicina e meropenem. Com relação à penicilina, a resistência apresentada por 3 amostras no teste de difusão, não foi confirmada pelo teste de diluição. A resistência concomitante a clindamicina e eritromicina foi verificada em 2 amostras; estas apresentaram o fenótipo MLSB constitutivo. A amostra resistente apenas a eritromicina apresentou o fenótipo M. A incidência relativamente elevada (19,2%) de colonização por S.agalactiae entre as gestantes estudadas e o isolamento de amostras resistentes aos antibióticos recomendados nos casos de alergia à penicilina, enfatizam a importância de detectar esta colonização no final da gravidez assim como de avaliar a susceptibilidade de S.agalactiae aos antimicrobianos, para uma correta prevenção da infecção neonatal.

Advisors/Committee Members: Silvia Susana Bona de Mondino, Aloysio de Mello Figueiredo Cerqueira, Rosana Rocha Barros, Claudia Rezende Vieira de Mendonça Souza, Angela Christina Dias de Castro, Flávio Augusto Prado Vasquez, Marinela Silva Laporte.

Subjects/Keywords: BACTEROLOGIA; Medicina; Streptococcus agalactiae; Colonization in pregnant women

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Borger, I. L. (2005). Estudo da Colonização por Streptococcus Agalactiae em Gestantes atendidas na Maternidade Escola da Universidade Federal do Rio de Janeiro. (Thesis). Universidade Federal Fluminense. Retrieved from http://www.bdtd.ndc.uff.br/tde_busca/arquivo.php?codArquivo=310

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Borger, Irina Lermontov. “Estudo da Colonização por Streptococcus Agalactiae em Gestantes atendidas na Maternidade Escola da Universidade Federal do Rio de Janeiro.” 2005. Thesis, Universidade Federal Fluminense. Accessed January 16, 2021. http://www.bdtd.ndc.uff.br/tde_busca/arquivo.php?codArquivo=310.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Borger, Irina Lermontov. “Estudo da Colonização por Streptococcus Agalactiae em Gestantes atendidas na Maternidade Escola da Universidade Federal do Rio de Janeiro.” 2005. Web. 16 Jan 2021.

Vancouver:

Borger IL. Estudo da Colonização por Streptococcus Agalactiae em Gestantes atendidas na Maternidade Escola da Universidade Federal do Rio de Janeiro. [Internet] [Thesis]. Universidade Federal Fluminense; 2005. [cited 2021 Jan 16]. Available from: http://www.bdtd.ndc.uff.br/tde_busca/arquivo.php?codArquivo=310.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Borger IL. Estudo da Colonização por Streptococcus Agalactiae em Gestantes atendidas na Maternidade Escola da Universidade Federal do Rio de Janeiro. [Thesis]. Universidade Federal Fluminense; 2005. Available from: http://www.bdtd.ndc.uff.br/tde_busca/arquivo.php?codArquivo=310

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

28. GlÃucia Frasnelli Mian. Diversidade populacional, vias de transmissÃo e virulÃncia de Streptococcus agalactiae isolados de tilÃpias do Nilo (Oreochromis niloticus).

Degree: 2009, UNIVERSIDADE FEDERAL DE LAVRAS

URL: http://bibtede.ufla.br/tede//tde_busca/arquivo.php?codArquivo=1895

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Streptococcus agalactiae is an emerging pathogen in Nile tilapia. To investigate aspects of the epidemiology, transmission, virulence and the genetic diversity of S. agalactiae infections,… (more)

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Streptococcus agalactiae is an emerging pathogen in Nile tilapia. To investigate aspects of the epidemiology, transmission, virulence and the genetic diversity of S. agalactiae infections, nine outbreaks of meningoencephalitis and septicemia in Nile tilapia farms in Brazil were analyzed. The 16S rRNA gene and ISR were utilized with molecular marker of the genetic variability. Records from the outbreaks revealed large variation in the weight of fish affected, high mortality, and disease occurrence at water temperatures above 27ÂC. S. agalactiae was isolated from diseased fish from all farms, and 29 strains were identified by phenotypic tests and 16S rRNA gene sequencing. All strains were highly virulent; for example, strain SA20-06 had an LD50 of 6.14Â101.17 CFU. Considerable variation in biochemical profile was observed and 13 different phenotypic variants were finding. To investigate S. agalactiae transmission, we conducted cohabitation assays with diseased and healthy fish and fish challenges using an immersion bath or branchial inoculation. The disease was reproduced with characteristic clinical signs and S. agalactiae was reisolated in all trials. The phylogenetic analyzes showed five different profiles for the sequences of 16S rRNA gene, presenting significant variability to the 29 strains of S. agalactiae evaluated. Different genetic patterns were observed in distinct isolates of the same farm. This variability was not compatible with the phenotypic variety described here, as well as with virulence of the strains of the S. agalactiae. The ISR showed to be highly conserved among the bacterial fish isolates.

Streptococcus agalactiae Ã um importante patÃgeno para tilÃpia do Nilo no mundo todo Para investigar os aspectos de epidemiologia transmissÃo virulÃncia e a diversidade genÃtica de S. agalactiae nove surtos de meningoencefalite e septicemia em tilÃpias do Nilo no Brasil foram analisados O gene 16S rRNA e o ISR foram utilizados como marcadores moleculares para a variabilidade genÃtica Dados sobre os surtos revelaram grande variaÃÃo no peso dos peixes afetados alta mortalidade e a ocorrÃncia da doenÃa em temperaturas acima de 27ÂC S agalactiae foi isolado de peixes doentes em todas as fazendas e 29 amostras foram identificadas por testes fenotÃpicos e seqÃenciamento do gene 16S rRNA Todos os isolados foram altamente virulentos por exemplo a amostra SA 20-06 apresentou uma DL50 de 6.14Â101.17 UFC ConsiderÃveis variaÃÃes no perfil bioquÃmico foram observadas e 13 variantes fenotÃpicos diferentes foram encontrados Para investigar a transmissÃo nÃs conduzimos experimentos de coabitaÃÃo com peixes doentes e peixes saudÃveis e peixes desafiados usando banho de imersÃo ou inoculaÃÃo em brÃnquia A doenÃa foi reproduzida com sinais clÃnicos caracterÃsticos e S agalactiae foi reisolado de todas as triagens As analises filogenÃticas mostraram 5 diferentes perfis para as seqÃÃncias do gene 16S rRNA apresentando significante variabilidade nas 29 amostras de S agalactiae avaliadas Diferentes padrÃes genÃticos foram…

Advisors/Committee Members: Ivi Cristina Menezes de Oliveira, Luciana InÃcia Gomes, Henrique CÃsar Pereira Figueiredo, Adriana Mello Garcia, Geraldo Marcio da Costa.

Subjects/Keywords: ISR; infecÃÃo experimental; 16SrRNA; AQUICULTURA; TilÃpias; Brasil; Streptococcus agalactiae

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mian, G. F. (2009). Diversidade populacional, vias de transmissÃo e virulÃncia de Streptococcus agalactiae isolados de tilÃpias do Nilo (Oreochromis niloticus). (Thesis). UNIVERSIDADE FEDERAL DE LAVRAS. Retrieved from http://bibtede.ufla.br/tede//tde_busca/arquivo.php?codArquivo=1895

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mian, GlÃucia Frasnelli. “Diversidade populacional, vias de transmissÃo e virulÃncia de Streptococcus agalactiae isolados de tilÃpias do Nilo (Oreochromis niloticus).” 2009. Thesis, UNIVERSIDADE FEDERAL DE LAVRAS. Accessed January 16, 2021. http://bibtede.ufla.br/tede//tde_busca/arquivo.php?codArquivo=1895.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mian, GlÃucia Frasnelli. “Diversidade populacional, vias de transmissÃo e virulÃncia de Streptococcus agalactiae isolados de tilÃpias do Nilo (Oreochromis niloticus).” 2009. Web. 16 Jan 2021.

Vancouver:

Mian GF. Diversidade populacional, vias de transmissÃo e virulÃncia de Streptococcus agalactiae isolados de tilÃpias do Nilo (Oreochromis niloticus). [Internet] [Thesis]. UNIVERSIDADE FEDERAL DE LAVRAS; 2009. [cited 2021 Jan 16]. Available from: http://bibtede.ufla.br/tede//tde_busca/arquivo.php?codArquivo=1895.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mian GF. Diversidade populacional, vias de transmissÃo e virulÃncia de Streptococcus agalactiae isolados de tilÃpias do Nilo (Oreochromis niloticus). [Thesis]. UNIVERSIDADE FEDERAL DE LAVRAS; 2009. Available from: http://bibtede.ufla.br/tede//tde_busca/arquivo.php?codArquivo=1895

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade de Lisboa

29. Silva, João Guilherme do Patrocínio Guerreiro da. Streptococcus agalactiae : um caso de meningite e endocardite num homem adulto.

Degree: 2017, Universidade de Lisboa

URL: https://www.rcaap.pt/detail.jsp?id=oai:repositorio.ul.pt:10451/31709

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Trabalho Final do Curso de Mestrado Integrado em Medicina, Faculdade de Medicina, Universidade de Lisboa, 2017

Streptococcus agalactiae não é um organismo desconhecido da maioria… (more)

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Trabalho Final do Curso de Mestrado Integrado em Medicina, Faculdade de Medicina, Universidade de Lisboa, 2017

Streptococcus agalactiae não é um organismo desconhecido da maioria dos médicos e estudantes de medicina. Contudo, a sua importância é quase sempre pensada apenas no contexto da ginecologia e obstetrícia: infeções da mãe e do recémnascido durante o período perinatal. Nos países desenvolvidos, nos últimos 20 anos, devido à forte aposta em programas de rastreio pré-natal de rotina nas grávidas, o número de casos de infeção em recém-nascidos diminuiu, sendo o grupo dos adultos o que representa a maior proporção de doença invasiva atualmente. É uma importante mudança de paradigma para a qual os profissionais de saúde devem estar alertados. Apresenta-se o caso de um homem adulto que desenvolveu um quadro de meningite e endocardite por Streptococcus agalactiae. São também abordados conceitos teóricos no campo da epidemiologia, fatores de risco, microbiologia e abordagem terapêutica. O interesse deste trabalho reside na conjugação de duas entidades clínicas, meningite e endocardite, por Streptococcus agalactiae no adulto não grávido.

Streptococcus agalactiae is not an unknown entity for most physicians and medical students. However, its importance is mainly considered in an obstetrics and gynecology setting: infections of the mother and the newborn during the perinatal period. Fourtunately, since the widespread institution of prenatal screening in developed countries in the last 20 years, the incidence of neonatal infection has fallen; due to this fact, adults now account for a larger proportion of invasive disease than do newborns. This represents an important paradigm shift, of which health care professionals should be aware. We report a case of an adult male who presented with meningitis and endocarditis caused by Streptococcus agalactiae. Important concepts regarding epidemiology, risk factors, microbiology and management approach are also discussed. This work aims at exploring the conjugation of two clinical entities, meningitis and endocarditis, caused by Streptococcus agalactiae in the non pregnant adult.

Advisors/Committee Members: Zagalo, Alexandra.

Subjects/Keywords: Streptococcus agalactiae; Meningite; Endocardite; Doenças transmissíveis; Domínio/Área Científica::Ciências Médicas

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Silva, J. G. d. P. G. d. (2017). Streptococcus agalactiae : um caso de meningite e endocardite num homem adulto. (Thesis). Universidade de Lisboa. Retrieved from https://www.rcaap.pt/detail.jsp?id=oai:repositorio.ul.pt:10451/31709

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Silva, João Guilherme do Patrocínio Guerreiro da. “Streptococcus agalactiae : um caso de meningite e endocardite num homem adulto.” 2017. Thesis, Universidade de Lisboa. Accessed January 16, 2021. https://www.rcaap.pt/detail.jsp?id=oai:repositorio.ul.pt:10451/31709.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Silva, João Guilherme do Patrocínio Guerreiro da. “Streptococcus agalactiae : um caso de meningite e endocardite num homem adulto.” 2017. Web. 16 Jan 2021.

Vancouver:

Silva JGdPGd. Streptococcus agalactiae : um caso de meningite e endocardite num homem adulto. [Internet] [Thesis]. Universidade de Lisboa; 2017. [cited 2021 Jan 16]. Available from: https://www.rcaap.pt/detail.jsp?id=oai:repositorio.ul.pt:10451/31709.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Silva JGdPGd. Streptococcus agalactiae : um caso de meningite e endocardite num homem adulto. [Thesis]. Universidade de Lisboa; 2017. Available from: https://www.rcaap.pt/detail.jsp?id=oai:repositorio.ul.pt:10451/31709

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Nova

30. Damião, Vera Alexandra Quintino. Produção de DNases extracelulares em estirpes de Streptococcus agalactiae de origem humana e bovina.

Degree: 2012, Universidade Nova

URL: http://www.rcaap.pt/detail.jsp?id=oai:run.unl.pt:10362/8434

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Dissertação para obtenção do Grau de Mestre em Genética Molecular e Biomedicina

Streptococcus agalactiae constitui uma das principais causas de pneumonia, septicémia e meningite em… (more)

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Dissertação para obtenção do Grau de Mestre em Genética Molecular e Biomedicina

Streptococcus agalactiae constitui uma das principais causas de pneumonia, septicémia e meningite em recém-nascidos e infecção emergente em adultos com patologia associada. A secreção de DNases é considerada vantajosa na disseminação bacteriana e na evasão à imunidade inata do hospedeiro, no entanto, apesar do elevado número de estirpes de S. agalactiae produtoras de DNases, não é evidente o seu envolvimento na patogénese desta bactéria. O presente estudo teve como principal objectivo correlacionar a actividade das DNases com outras variáveis em estudo, tais como, genótipo da estirpe, a sua origem clínica (colonização ou infecção) e o hospedeiro (humano ou bovino), por forma a contribuir para o esclarecimento desta temática. Uma colecção de 345 estirpes de S. agalactiae foi caracterizada por serotipagem, MLST (“Multilocus Sequence Typing”) para identificação de sequências tipo (ST), perfis MLVA (“Multiple-Locus Variant-Repeat Assay”), padrão de resistência a antimicrobianos, elementos móveis e proteínas de superfície, padrão de restrição por PFGE (“Pulsed-Field Gel Electrophoresis”) e a actividade das DNases. As estirpes de origem bovina (n=60; 100%) e a maioria (n=285; 86%) das estirpes de origem humana foram produtoras de DNases. As estirpes não produtoras apresentaram a mesma linhagem genética: CC19 (tipos II, III-1 e V). Foram identificados dois perfis MLVA nas estirpes CC19 (perfil 32 em estirpes ST28 e perfil 33 nas restantes). Verificou-se que 31% das estirpes CC19 de colonização revelaram resistência à eritromicina e 16.4% apresentaram resistência à eritromicina e clindamicina. Nas estirpes ST28 foi identificado o elemento móvel GBSi1 e nas restantes estirpes CC19 foi detectado o elemento móvel IS1548. Em todas as estirpes, excepto uma foi detectado o gene rib. Foram identificados cinco padrões de PFGE, concluindo-se que a maioria das estirpes era clonal. O presente estudo permitiu evidenciar que o facto de uma estirpe pertencer ao CC19 aumenta a probabilidade de não produzir DNases.

Advisors/Committee Members: Sanches, Ilda, Borrego, Maria José.

Subjects/Keywords: Streptococcus agalactiae; DNases extracelulares; Genótipo; Origem clínica; Hospedeiro

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Damião, V. A. Q. (2012). Produção de DNases extracelulares em estirpes de Streptococcus agalactiae de origem humana e bovina. (Thesis). Universidade Nova. Retrieved from http://www.rcaap.pt/detail.jsp?id=oai:run.unl.pt:10362/8434

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Damião, Vera Alexandra Quintino. “Produção de DNases extracelulares em estirpes de Streptococcus agalactiae de origem humana e bovina.” 2012. Thesis, Universidade Nova. Accessed January 16, 2021. http://www.rcaap.pt/detail.jsp?id=oai:run.unl.pt:10362/8434.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Damião, Vera Alexandra Quintino. “Produção de DNases extracelulares em estirpes de Streptococcus agalactiae de origem humana e bovina.” 2012. Web. 16 Jan 2021.

Vancouver:

Damião VAQ. Produção de DNases extracelulares em estirpes de Streptococcus agalactiae de origem humana e bovina. [Internet] [Thesis]. Universidade Nova; 2012. [cited 2021 Jan 16]. Available from: http://www.rcaap.pt/detail.jsp?id=oai:run.unl.pt:10362/8434.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Damião VAQ. Produção de DNases extracelulares em estirpes de Streptococcus agalactiae de origem humana e bovina. [Thesis]. Universidade Nova; 2012. Available from: http://www.rcaap.pt/detail.jsp?id=oai:run.unl.pt:10362/8434

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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Open Access Theses and Dissertations