subject:(Steroidogenic factor 1)

Universidade Estadual de Campinas

1. Fabbri-Scallet, Helena, 1987-. Estudo funcional de novas variações nucleotídicas no gene NR5A1 em pacientes 46,XY com distúrbios da diferenciação do sexo: Functional analyses of new nucleotide variations in NR5A1 gene in patients 46,XY with disorders of sex development.

Degree: 2017, Universidade Estadual de Campinas

URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/330312

► Abstract: Disorders of Sex Development (DSD) are defined as congenital conditions in which development of chromosomal, gonadal or anatomical sex is atypical. Mutations in the… (more)

Subjects/Keywords: Fator esteroidogênico 1; Disgenesia gonadal; Mutação; Steroidogenic factor 1; Gonadal dysgenesis; Mutation

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Fabbri-Scallet, Helena, 1. (2017). Estudo funcional de novas variações nucleotídicas no gene NR5A1 em pacientes 46,XY com distúrbios da diferenciação do sexo: Functional analyses of new nucleotide variations in NR5A1 gene in patients 46,XY with disorders of sex development. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/330312

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Fabbri-Scallet, Helena, 1987-. “Estudo funcional de novas variações nucleotídicas no gene NR5A1 em pacientes 46,XY com distúrbios da diferenciação do sexo: Functional analyses of new nucleotide variations in NR5A1 gene in patients 46,XY with disorders of sex development.” 2017. Thesis, Universidade Estadual de Campinas. Accessed January 18, 2021. http://repositorio.unicamp.br/jspui/handle/REPOSIP/330312.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Fabbri-Scallet, Helena, 1987-. “Estudo funcional de novas variações nucleotídicas no gene NR5A1 em pacientes 46,XY com distúrbios da diferenciação do sexo: Functional analyses of new nucleotide variations in NR5A1 gene in patients 46,XY with disorders of sex development.” 2017. Web. 18 Jan 2021.

Vancouver:

Fabbri-Scallet, Helena 1. Estudo funcional de novas variações nucleotídicas no gene NR5A1 em pacientes 46,XY com distúrbios da diferenciação do sexo: Functional analyses of new nucleotide variations in NR5A1 gene in patients 46,XY with disorders of sex development. [Internet] [Thesis]. Universidade Estadual de Campinas; 2017. [cited 2021 Jan 18]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/330312.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Fabbri-Scallet, Helena 1. Estudo funcional de novas variações nucleotídicas no gene NR5A1 em pacientes 46,XY com distúrbios da diferenciação do sexo: Functional analyses of new nucleotide variations in NR5A1 gene in patients 46,XY with disorders of sex development. [Thesis]. Universidade Estadual de Campinas; 2017. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/330312

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universiteit Utrecht

2. Koch, R.D. Mutation analysis of the NR5A1 and P53 tumor suppressor gene of normal adrenals and cortisol-secreting adrenocortical tumors in dogs.

Degree: 2014, Universiteit Utrecht

URL: http://dspace.library.uu.nl:8080/handle/1874/302268

► The steroidogenic factor 1 (SF-1) plays an important role in adrenal development in humans and mice and there are also indications that the regulation of… (more)

Subjects/Keywords: adrenocortical tumors; steroidogenic factor 1; NR5A1 gene; P53 tumor supressor gene; mutation analysis

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Koch, R. D. (2014). Mutation analysis of the NR5A1 and P53 tumor suppressor gene of normal adrenals and cortisol-secreting adrenocortical tumors in dogs. (Masters Thesis). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/302268

Chicago Manual of Style (16^th Edition):

Koch, R D. “Mutation analysis of the NR5A1 and P53 tumor suppressor gene of normal adrenals and cortisol-secreting adrenocortical tumors in dogs.” 2014. Masters Thesis, Universiteit Utrecht. Accessed January 18, 2021. http://dspace.library.uu.nl:8080/handle/1874/302268.

MLA Handbook (7^th Edition):

Koch, R D. “Mutation analysis of the NR5A1 and P53 tumor suppressor gene of normal adrenals and cortisol-secreting adrenocortical tumors in dogs.” 2014. Web. 18 Jan 2021.

Vancouver:

Koch RD. Mutation analysis of the NR5A1 and P53 tumor suppressor gene of normal adrenals and cortisol-secreting adrenocortical tumors in dogs. [Internet] [Masters thesis]. Universiteit Utrecht; 2014. [cited 2021 Jan 18]. Available from: http://dspace.library.uu.nl:8080/handle/1874/302268.

Council of Science Editors:

Koch RD. Mutation analysis of the NR5A1 and P53 tumor suppressor gene of normal adrenals and cortisol-secreting adrenocortical tumors in dogs. [Masters Thesis]. Universiteit Utrecht; 2014. Available from: http://dspace.library.uu.nl:8080/handle/1874/302268

3. Guedes, Dulce Rondina. Estudo investigativo clínico, laboratorial, patológico, morfométrico, molecular de 10 pacientes com pseudohermafroditismo masculino disgenético (ADS 46, XY).

Degree: PhD, Pediatria, 2010, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/5/5141/tde-10032010-105027/ ;

►

O Pseudohermafroditismo masculino disgenético (Anomalia da diferenciação sexual 46,XY ADS 46,XY) é definido como ambigüidade genital num paciente com testículos e/ou cariótipo 46,XY com uma… (more)

▼

O Pseudohermafroditismo masculino disgenético (Anomalia da diferenciação sexual 46,XY ADS 46,XY) é definido como ambigüidade genital num paciente com testículos e/ou cariótipo 46,XY com uma das seguintes características: alteração histológica testicular, ausência ou hipoplasia das células de Leydig em tecido previamente estimulado com gonadotrofina coriônica humana(hCG), falta de resposta de testosterona ao estímulo com hCG sem acúmulo de precursores, ausência de células germinativas, presença de derivados müllerianos indicando inadequada produção do hormônio antiMülleriano (HAM) ou resistência de seus receptores. Esse estudo apresenta uma avaliação clínica, laboratorial, anátomopatológica, morfométrica e molecular de 10 pacientes com ADS 46,XY; dois pacientes apresentaram mutação no SF1 (fator esteroidogênico 1), duas mutações no domínio hingee uma terceira produziu um stop códon na posição 404; três pacientes com deleção da cópia do DAZ2. A morfometria testicular mostrou todos os diâmetros tubulares médios (DTM) moderado a gravemente diminuídos e os índices de fertilidade tubular leve a moderadamente diminuídos. Devido à dificuldade do diagnóstico diferencial e etiológico, o estudo morfométrico e molecular deve sempre acompanhar esses casos de ADS 46,XY.

The dysgenetic male pseudohermaphroditism 46,XY ; disorders of sex development (DSD 46,XY) is defined as sexual ambiguity in patients with testis and/or 46,XY karyotype and one of the characteristics: hystologic alteration of the testis; absence or hypoplasia of Leydig cells; a decreased testosterone response to human chorionic gonadotropin stimulation without accumulation of testosterone precursors; absent germ cells; presence of müllerian duct derivatives showing inappropriate production of antimüllerian hormone (AMH) or resistance to its receptors. This study shows the clinic, laboratory, histologic, morphometric and molecular evaluation of 10 patients with DSD 46,XY; two patients showed mutations in the SF1 gene (steroidogenic factor-1); two in the hinge domain and one stop codon at the position 404 of the protein; three patients exhibited deletion of DAZ2. The testis morphometry showed reduction: marked to severe of all mean tubular diameter (MTD) while the reduction of the tubular fertility index (TFI) were slight to marked. Due to difficulties establishing the differential diagnosis and the etiology, the morphometric and molecular evaluation must be always done in the patients with DSD 46,XY.

Advisors/Committee Members: Damiani, Durval.

Subjects/Keywords: Células germinativas; Disgenesia gonadal; Fator esteroidogênico 1; Germ cells; Gonadal dysgenesis; Pseudo-hermafroditismo; Pseudohermaphroditism; Seminiferous tubules; Steroidogenic factor-1; Túbulos seminíferos

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Guedes, D. R. (2010). Estudo investigativo clínico, laboratorial, patológico, morfométrico, molecular de 10 pacientes com pseudohermafroditismo masculino disgenético (ADS 46, XY). (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/5/5141/tde-10032010-105027/ ;

Chicago Manual of Style (16^th Edition):

Guedes, Dulce Rondina. “Estudo investigativo clínico, laboratorial, patológico, morfométrico, molecular de 10 pacientes com pseudohermafroditismo masculino disgenético (ADS 46, XY).” 2010. Doctoral Dissertation, University of São Paulo. Accessed January 18, 2021. http://www.teses.usp.br/teses/disponiveis/5/5141/tde-10032010-105027/ ;.

MLA Handbook (7^th Edition):

Guedes, Dulce Rondina. “Estudo investigativo clínico, laboratorial, patológico, morfométrico, molecular de 10 pacientes com pseudohermafroditismo masculino disgenético (ADS 46, XY).” 2010. Web. 18 Jan 2021.

Vancouver:

Guedes DR. Estudo investigativo clínico, laboratorial, patológico, morfométrico, molecular de 10 pacientes com pseudohermafroditismo masculino disgenético (ADS 46, XY). [Internet] [Doctoral dissertation]. University of São Paulo; 2010. [cited 2021 Jan 18]. Available from: http://www.teses.usp.br/teses/disponiveis/5/5141/tde-10032010-105027/ ;.

Council of Science Editors:

Guedes DR. Estudo investigativo clínico, laboratorial, patológico, morfométrico, molecular de 10 pacientes com pseudohermafroditismo masculino disgenético (ADS 46, XY). [Doctoral Dissertation]. University of São Paulo; 2010. Available from: http://www.teses.usp.br/teses/disponiveis/5/5141/tde-10032010-105027/ ;

University of Texas Southwestern Medical Center

4. Kim, Ki Woo. The Functional Roles of Steroidogenic Factor 1 in the Ventromedial Nucleus of the Hypothalamus.

Degree: 2009, University of Texas Southwestern Medical Center

URL: http://hdl.handle.net/2152.5/548

► Steroidogenic factor 1 (SF-1) is a nuclear receptor that plays important roles in the hypothalamic-pituitary-steroidogenic organ axis. Global knockout studies in mice revealed the essential… (more)

Subjects/Keywords: Steroidogenic Factor 1; Ventromedial Hypothalamic Nucleus; Gene Expression Regulation

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kim, K. W. (2009). The Functional Roles of Steroidogenic Factor 1 in the Ventromedial Nucleus of the Hypothalamus. (Thesis). University of Texas Southwestern Medical Center. Retrieved from http://hdl.handle.net/2152.5/548

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kim, Ki Woo. “The Functional Roles of Steroidogenic Factor 1 in the Ventromedial Nucleus of the Hypothalamus.” 2009. Thesis, University of Texas Southwestern Medical Center. Accessed January 18, 2021. http://hdl.handle.net/2152.5/548.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kim, Ki Woo. “The Functional Roles of Steroidogenic Factor 1 in the Ventromedial Nucleus of the Hypothalamus.” 2009. Web. 18 Jan 2021.

Vancouver:

Kim KW. The Functional Roles of Steroidogenic Factor 1 in the Ventromedial Nucleus of the Hypothalamus. [Internet] [Thesis]. University of Texas Southwestern Medical Center; 2009. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/2152.5/548.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kim KW. The Functional Roles of Steroidogenic Factor 1 in the Ventromedial Nucleus of the Hypothalamus. [Thesis]. University of Texas Southwestern Medical Center; 2009. Available from: http://hdl.handle.net/2152.5/548

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Texas Southwestern Medical Center

5. Stallings, Nancy Ruth. Multiple Approaches to Study of Steroidogenic Factor 1 : Identification of a Novel Regulatory Element and Identification of Novel Target Genes.

Degree: 2005, University of Texas Southwestern Medical Center

URL: http://hdl.handle.net/2152.5/530

► Steroidogenic Factor 1 (SF-1) is an essential component of the hypothalamic-pituitary-adrenal-gonadal axis. SF-1 knockout (KO) mice lack adrenals, gonads, the ventromedial hypothalamic nucleus (VMH), and… (more)

Subjects/Keywords: DNA-Binding Proteins; Steroidogenic Factor 1; Endocrine System

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Stallings, N. R. (2005). Multiple Approaches to Study of Steroidogenic Factor 1 : Identification of a Novel Regulatory Element and Identification of Novel Target Genes. (Thesis). University of Texas Southwestern Medical Center. Retrieved from http://hdl.handle.net/2152.5/530

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Stallings, Nancy Ruth. “Multiple Approaches to Study of Steroidogenic Factor 1 : Identification of a Novel Regulatory Element and Identification of Novel Target Genes.” 2005. Thesis, University of Texas Southwestern Medical Center. Accessed January 18, 2021. http://hdl.handle.net/2152.5/530.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Stallings, Nancy Ruth. “Multiple Approaches to Study of Steroidogenic Factor 1 : Identification of a Novel Regulatory Element and Identification of Novel Target Genes.” 2005. Web. 18 Jan 2021.

Vancouver:

Stallings NR. Multiple Approaches to Study of Steroidogenic Factor 1 : Identification of a Novel Regulatory Element and Identification of Novel Target Genes. [Internet] [Thesis]. University of Texas Southwestern Medical Center; 2005. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/2152.5/530.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Stallings NR. Multiple Approaches to Study of Steroidogenic Factor 1 : Identification of a Novel Regulatory Element and Identification of Novel Target Genes. [Thesis]. University of Texas Southwestern Medical Center; 2005. Available from: http://hdl.handle.net/2152.5/530

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Toronto

6. Rui, Xianliang. Transcriptional Regulation of the Mouse Adrenal Cyclase Type 4 (Adcy4) in Y1 Adrenocortical Tumor Cells.

Degree: 2010, University of Toronto

URL: http://hdl.handle.net/1807/24451

►

Adenylyl cyclase (Adcy) is an important early effector of adrenocorticotrophin (ACTH) on the adrenal cortex; however, this enzyme consists of ten isozymes in mammalian cells… (more)

▼

Adenylyl cyclase (Adcy) is an important early effector of adrenocorticotrophin (ACTH) on the adrenal cortex; however, this enzyme consists of ten isozymes in mammalian cells and the factors governing the expression of different Adcy isozymes have not been well defined. The aim of this study is to investigate the regulation of mouse Adcy4, one of ten isozymes, in Y1 adrenocortical tumor cells and in mutant subclones derived from the Y1 cells. Adcy4 is expressed at a high level in brain but at lower levels in many other tissues including the Y1 cells. Moreover, this isozyme is specifically deficient in Y1 mutants with impaired steroidogenic factor 1 (SF1) activity. These observations support a hypothesis that Adcy4 expression is influenced by both ubiquitously expressed and tissue-specific transcription factors. My sequencing results indicate that mouse Adcy4 is highly homologous to the human and rat counterparts; its gene is located less than 1 kb downstream of Ripk3 and contains 26 exons. Primer extension and in silico analyses suggest that Adcy4 contains a TATA-less promoter and initiates transcription from multiple sites. Luciferase reporter gene assays indicate that Adcy4 promoter activity is mainly stimulated by the proximal GC-rich region but is inhibited by the first intron. This 124 bp GC-rich region is well conserved among several mammalian species and exhibits strong promoter activity in Y1 cells, which is functionally compromised in the Adcy4-deficient mutant. Within this region, three Sp1/Sp3- and one SF1-binding sites have been identified which bind the corresponding proteins Sp1 and Sp3 or SF1 in electrophoretic mobility shift assays (EMSAs). Site-directed mutagenesis reveals that the 5’-most Sp1/Sp3 site enhances Adcy4 promoter activity, whereas the middle Sp1/Sp3 and SF1 sites each repress this activity. In Y1 mutant cells, mutating the SF1 site restores Adcy4 promoter activity and knocking down SF1 with shRNA increases Adcy4 expression. All these data demonstrate that Adcy4 expression is under the control of the ubiquitous factors Sp1 and Sp3 and the tissue-specific factor SF1 and establish that SF1 is a repressor for Adcy4 promoter activity. This study is the first to demonstrate a repressor function for SF1 in certain promoter contexts.

PhD

Advisors/Committee Members: Schimmer, Bernard P., Pharmacology.

Subjects/Keywords: Adenylyl cyclase; Steroidogenesis; Steroidogenic factor 1 (SF1); Gene regulation; Pharmacology 0419

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Rui, X. (2010). Transcriptional Regulation of the Mouse Adrenal Cyclase Type 4 (Adcy4) in Y1 Adrenocortical Tumor Cells. (Doctoral Dissertation). University of Toronto. Retrieved from http://hdl.handle.net/1807/24451

Chicago Manual of Style (16^th Edition):

Rui, Xianliang. “Transcriptional Regulation of the Mouse Adrenal Cyclase Type 4 (Adcy4) in Y1 Adrenocortical Tumor Cells.” 2010. Doctoral Dissertation, University of Toronto. Accessed January 18, 2021. http://hdl.handle.net/1807/24451.

MLA Handbook (7^th Edition):

Rui, Xianliang. “Transcriptional Regulation of the Mouse Adrenal Cyclase Type 4 (Adcy4) in Y1 Adrenocortical Tumor Cells.” 2010. Web. 18 Jan 2021.

Vancouver:

Rui X. Transcriptional Regulation of the Mouse Adrenal Cyclase Type 4 (Adcy4) in Y1 Adrenocortical Tumor Cells. [Internet] [Doctoral dissertation]. University of Toronto; 2010. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/1807/24451.

Council of Science Editors:

Rui X. Transcriptional Regulation of the Mouse Adrenal Cyclase Type 4 (Adcy4) in Y1 Adrenocortical Tumor Cells. [Doctoral Dissertation]. University of Toronto; 2010. Available from: http://hdl.handle.net/1807/24451

Brigham Young University

7. McIlmoil, Stephen A. Regulation of Adrenal Steroidogenesis by Interleukin-6.

Degree: MS, 2007, Brigham Young University

URL: https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=1974&context=etd

► Cortisol and dehydroepiandrosterone (DHEA) are steroids produced by the zona fasciculata (ZF) and reticularis (ZR), respectively, of the adrenal cortex. Both steroids are upregulated in… (more)

▼ Cortisol and dehydroepiandrosterone (DHEA) are steroids produced by the zona fasciculata (ZF) and reticularis (ZR), respectively, of the adrenal cortex. Both steroids are upregulated in response to adrenocorticotropic hormone (ACTH). Cortisol is a glucorticoid that is important in the regulation of inflammation and metabolism. DHEA is an adrenal androgen important in fetal growth and puberty but tends to decrease gradually after puberty in both men and women. DHEA has various effects on metabolism and immune function including inhibiting the effects of cortisol on some tissues. During the acute phase of stress, cortisol and DHEA rise due to an increase in ACTH released from the anterior pituitary. In contrast, during chronic stress, cortisol remains elevated but DHEA and ACTH levels decrease. Likewise, stress causes serum levels of IL-6 to increase. IL-6 increases cortisol release from the human and bovine adrenal cortex. IL-6 also decreases DHEA release from zona reticularis of the bovine adrenal gland. In humans the effect of IL-6 on DHEA production is still uncertain. To determine a possible mechanism of IL-6 on the zona fasciculata and reticularis, human H294R cells and bovine adrenal tissue were incubated in serum free medium containing IL-6, at various concentrations and incubation intervals. At the end of the incubation interval, mRNA or protein was extracted from the cells or tissue. Standard PCR, real time PCR, and western blot assays were used to determine the effects of IL-6 on the enzymes involved in cortisol and DHEA synthesis, steroidogenic factor-1 (SF-1), steroidogenic acute regulatory protein (StAR), and dosage sensitive sex reversal adrenal hypoplasia congenita critical region on the X chromosome, gene 1 (DAX-1). In human H295R cells and bovine zona fasciculata cells IL-6 caused an increase in SF-1, StAR, P450scc, 17α hydroxylase, 3β-hydroxysteroid dehydrogenase type 2 (3β HSD2), 21 hydroxylase, and 11β hydroxylase mRNA and protein. IL-6 caused DAX-1 mRNA and protein to decrease. These effects were manifest in a time dependent manner. Dose response treatments incubated for 60 min increased SF-1, StAR, P450scc, 17α hydroxylase, 3β HSD2, 21 hydroxylase, and 11β hydroxylase but there was not significant change between the different treatments of IL-6. The bovine zona reticularis stimulated with IL-6 showed a decrease in SF-1, StAR, P450scc, 17α hydroxylase, and 3β HSD2 with an increase in DAX-1 mRNA and protein. This response was manifest in a time dependent manner for both mRNA and protein, and the effect was dose-dependent for mRNA but not protein levels within the 60 min time period. These data provide a mechanism by which increased stress, physical or emotional, which increases IL-6 serum level, could increase cortisol and decrease DHEA. This would account for decreased immune function, increased blood pressure, and changes in metabolism.

Subjects/Keywords: Adrenal; Cortisol; Dehydroepiandrostendione; interleukin-6; steroidogenesis; zona fasciculata; zona reticularis; Steroidogenic factor-1; DAX-1; Cell and Developmental Biology; Physiology

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

McIlmoil, S. A. (2007). Regulation of Adrenal Steroidogenesis by Interleukin-6. (Masters Thesis). Brigham Young University. Retrieved from https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=1974&context=etd

Chicago Manual of Style (16^th Edition):

McIlmoil, Stephen A. “Regulation of Adrenal Steroidogenesis by Interleukin-6.” 2007. Masters Thesis, Brigham Young University. Accessed January 18, 2021. https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=1974&context=etd.

MLA Handbook (7^th Edition):

McIlmoil, Stephen A. “Regulation of Adrenal Steroidogenesis by Interleukin-6.” 2007. Web. 18 Jan 2021.

Vancouver:

McIlmoil SA. Regulation of Adrenal Steroidogenesis by Interleukin-6. [Internet] [Masters thesis]. Brigham Young University; 2007. [cited 2021 Jan 18]. Available from: https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=1974&context=etd.

Council of Science Editors:

McIlmoil SA. Regulation of Adrenal Steroidogenesis by Interleukin-6. [Masters Thesis]. Brigham Young University; 2007. Available from: https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=1974&context=etd

University of Michigan

8. Gummow, Brian Michael. Multiple signaling pathways regulate steroidogenic factor -1 activity through induction of co-factor availability.

Degree: PhD, Molecular biology, 2005, University of Michigan

URL: http://hdl.handle.net/2027.42/125417

► Steroidogenic Factor-1 (SF-1) is a monomer binding nuclear hormone receptor that is critical for adrenocortical and gonadal development. Further, SF-1 regulates the transcription of most… (more)

Subjects/Keywords: Activity; Adrenal Cortex; Availability; Co; Glucocorticoids; Induction; Multiple; Pathways; Regulate; Signaling; Steroidogenic Factor-1; Wnt

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Gummow, B. M. (2005). Multiple signaling pathways regulate steroidogenic factor -1 activity through induction of co-factor availability. (Doctoral Dissertation). University of Michigan. Retrieved from http://hdl.handle.net/2027.42/125417

Chicago Manual of Style (16^th Edition):

Gummow, Brian Michael. “Multiple signaling pathways regulate steroidogenic factor -1 activity through induction of co-factor availability.” 2005. Doctoral Dissertation, University of Michigan. Accessed January 18, 2021. http://hdl.handle.net/2027.42/125417.

MLA Handbook (7^th Edition):

Gummow, Brian Michael. “Multiple signaling pathways regulate steroidogenic factor -1 activity through induction of co-factor availability.” 2005. Web. 18 Jan 2021.

Vancouver:

Gummow BM. Multiple signaling pathways regulate steroidogenic factor -1 activity through induction of co-factor availability. [Internet] [Doctoral dissertation]. University of Michigan; 2005. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/2027.42/125417.

Council of Science Editors:

Gummow BM. Multiple signaling pathways regulate steroidogenic factor -1 activity through induction of co-factor availability. [Doctoral Dissertation]. University of Michigan; 2005. Available from: http://hdl.handle.net/2027.42/125417

University of Michigan

9. Scheys, Joshua O. Nuclear Receptors of the Adrenal Cortex: Genomic Targets of Steroidogenic Factor 1 and Evidence of Adrenal Failure in DAX1-Deficient Mice.

Degree: PhD, Cellular & Molecular Biology, 2010, University of Michigan

URL: http://hdl.handle.net/2027.42/78891

► Steroidogenic Factor 1 (SF1) and DAX1 are nuclear receptors that play an integral role in the adrenal cortex. SF1 is a transcription factor that regulates… (more)

▼ Steroidogenic Factor 1 (SF1) and DAX1 are nuclear receptors that play an integral role in the adrenal cortex. SF1 is a transcription factor that regulates expression of myriad genes, including those involved in steroidogenesis, production of various endocrine hormones, and development of the adrenals and gonads. DAX1 is an orphan nuclear receptor that inhibits SF1 and, when mutated, is responsible for X-linked Adrenal Hypoplasia Congenita (AHC). In the adrenal cortex, adrenocorticotropic hormone (ACTH) activates SF1-mediated transcription of the genes involved in cholesterol transport and steroid production. However, SF1 is also implicated in adrenal proliferation and differentiation; therefore, it is likely that several different signaling pathways regulate SF1-dependent transcription by altering cofactor availability or by feedback mechanisms that modulate signaling molecules that impinge on SF1 transactivation. This thesis describes three genomic targets of SF1 that provide feedback upon and affect subsequent SF1 transcriptional activity. First, I describe the identification of Edg5, the sphingosine 1-phosphate receptor, as a target of SF1 using chromatin immunoprecipitation-chip microarray (ChIP-chip). Activation of Edg5 may alter levels of intracellular sphingosine 1-phosphate, a ligand of SF1 that activates steroidogenesis and stimulates release of adrenal hormones. I also describe association of SF1 on the adenylyl cyclase 4 (Adcy4) promoter. Though usually an activator, SF1 represses transcription of Adcy4, which may alter cAMP-dependent activation of SF1. Finally, I describe an interaction between SF1 and GR to activate expression of Dax1, which is abrogated by ACTH. In all three cases, the genomic target of SF1 encodes a factor that can modulate SF1-dependent transcription. While loss of DAX1 in humans results in AHC, Dax1 deficient-mice have a mild adrenal phenotype. To reconcile these disparate phenotypes, I analyzed Dax1-deficient mice across their lifespan. Young Dax1-deficent mice have increased steroidogenic capacity, expression of steroidogenic enzymes, and proliferation. However, after 60 weeks all three measures are dramatically reduced, suggesting that loss of Dax1 may result in premature differentiation resulting in ultimate exhaustion of the adrenal cortex, which has implications for AHC. Overall, this dissertation contributes to the understanding of both SF1-dependent gene regulation and the significance of DAX1 in homeostasis of the adrenal cortex. Advisors/Committee Members: Hammer, Gary D. (committee member), Koenig, Ronald (committee member), Kwok, Roland P. (committee member), Robins, Diane M. (committee member), Seasholtz, Audrey F. (committee member).

Subjects/Keywords: Adrenal; Steroidogenic Factor 1; Dax1; Adrenal Hypoplasia Congenita; Molecular, Cellular and Developmental Biology; Science

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Scheys, J. O. (2010). Nuclear Receptors of the Adrenal Cortex: Genomic Targets of Steroidogenic Factor 1 and Evidence of Adrenal Failure in DAX1-Deficient Mice. (Doctoral Dissertation). University of Michigan. Retrieved from http://hdl.handle.net/2027.42/78891

Chicago Manual of Style (16^th Edition):

Scheys, Joshua O. “Nuclear Receptors of the Adrenal Cortex: Genomic Targets of Steroidogenic Factor 1 and Evidence of Adrenal Failure in DAX1-Deficient Mice.” 2010. Doctoral Dissertation, University of Michigan. Accessed January 18, 2021. http://hdl.handle.net/2027.42/78891.

MLA Handbook (7^th Edition):

Scheys, Joshua O. “Nuclear Receptors of the Adrenal Cortex: Genomic Targets of Steroidogenic Factor 1 and Evidence of Adrenal Failure in DAX1-Deficient Mice.” 2010. Web. 18 Jan 2021.

Vancouver:

Scheys JO. Nuclear Receptors of the Adrenal Cortex: Genomic Targets of Steroidogenic Factor 1 and Evidence of Adrenal Failure in DAX1-Deficient Mice. [Internet] [Doctoral dissertation]. University of Michigan; 2010. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/2027.42/78891.

Council of Science Editors:

Scheys JO. Nuclear Receptors of the Adrenal Cortex: Genomic Targets of Steroidogenic Factor 1 and Evidence of Adrenal Failure in DAX1-Deficient Mice. [Doctoral Dissertation]. University of Michigan; 2010. Available from: http://hdl.handle.net/2027.42/78891

Georgia Tech

10. Urs, Aarti N. Reciprocal binding of sphingosine and phosphatidic acid to steroidogenic factor 1 regulates the transcription of CYP17.

Degree: MS, Biology, 2005, Georgia Tech

URL: http://hdl.handle.net/1853/7638

► Steroidogenic factor (SF1) is an orphan nuclear receptor that is essential for steroid hormone-biosynthesis and endocrine development. Recent studies have demonstrated that phospholipids are ligands… (more)

Subjects/Keywords: CYP17; Sphingosine; Phosphatidic acids; Steroidogenic factor 1; Transcription factors; Steroid hormones Synthesis; Sphingosine; Pregnenolone; Phospholipids; Ligands

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Urs, A. N. (2005). Reciprocal binding of sphingosine and phosphatidic acid to steroidogenic factor 1 regulates the transcription of CYP17. (Masters Thesis). Georgia Tech. Retrieved from http://hdl.handle.net/1853/7638

Chicago Manual of Style (16^th Edition):

Urs, Aarti N. “Reciprocal binding of sphingosine and phosphatidic acid to steroidogenic factor 1 regulates the transcription of CYP17.” 2005. Masters Thesis, Georgia Tech. Accessed January 18, 2021. http://hdl.handle.net/1853/7638.

MLA Handbook (7^th Edition):

Urs, Aarti N. “Reciprocal binding of sphingosine and phosphatidic acid to steroidogenic factor 1 regulates the transcription of CYP17.” 2005. Web. 18 Jan 2021.

Vancouver:

Urs AN. Reciprocal binding of sphingosine and phosphatidic acid to steroidogenic factor 1 regulates the transcription of CYP17. [Internet] [Masters thesis]. Georgia Tech; 2005. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/1853/7638.

Council of Science Editors:

Urs AN. Reciprocal binding of sphingosine and phosphatidic acid to steroidogenic factor 1 regulates the transcription of CYP17. [Masters Thesis]. Georgia Tech; 2005. Available from: http://hdl.handle.net/1853/7638

11. Lucki, Natasha Chrystman. Characterization of the role of acid ceramidase in adrenocortical steroid hormone biosynthesis.

Degree: PhD, Biology, 2011, Georgia Tech

URL: http://hdl.handle.net/1853/42804

► Sphingolipids modulate multiple cellular functions, including steroid hormone biosynthesis. Sphingosine is an antagonist ligand for the nuclear receptor steroidogenic factor 1 (SF-1), which is the… (more)

▼ Sphingolipids modulate multiple cellular functions, including steroid hormone biosynthesis. Sphingosine is an antagonist ligand for the nuclear receptor steroidogenic factor 1 (SF-1), which is the primary transcriptional regulator of most steroidogenic genes. Furthermore, sphingosine-dependent repression of SF-1 function is dependent on the expression of acid ceramidase (ASAH1), an enzyme that forms sphingosine. Based on these data, I hypothesized that ACTH/cAMP signaling regulates ASAH1 function at both transcriptional and post-transcriptional levels. In addition, because SF-1 is predominantly a nuclear protein, I postulated that ASAH1 modulates SF-1 function and, therefore, steroidogenic gene expression by controlling the nuclear concentrations of SPH. To test these hypotheses, I first examined the effect of chronic ACTH/cAMP signaling on the transcription of the ASAH1 gene. Next, the functional significance of ASAH1 expression in adrenocortical cells was probed by generating an ASAH1-knockdown cell line. I subsequently characterized the role of ASAH1 as a transcriptional nuclear receptor coregulator. Finally, I defined the role of sphingosine-1-phosphate, a bi-product of ASAH1 activity, in the acute phase of cortisol biosynthesis. Using a variety of experimental approaches, I identified cAMP response element binding protein as an essential transcriptional activator of the ASAH1 gene. Analysis of adrenocortical cells lacking ASAH1 revealed that ASAH1 is a global regulator of steroidogenic capacity. Furthermore, I identified ASAH1 as a nuclear protein and defined the molecular determinants of the interaction between ASAH1 and SF-1. Collectively, this body of work establishes the integral role of ASAH1 in the regulation of ACTH-dependent adrenocortical cortisol biosynthesis. Advisors/Committee Members: Sewer, Marion (Committee Chair), Merrill, Al (Committee Co-Chair), Boyan, Barbara (Committee Member), Fan, Yuhong (Committee Member), McCarty, Nael (Committee Member).

Subjects/Keywords: Steroidogenic factor 1; Acid ceramidase; Sphingolipid; Adrenal gland; Cortisol; Sphingosine; Adrenal cortex; Adrenocortical hormones; Steroid hormones; Glycosphingolipids

…stress-activated protein kinase SF-1 – steroidogenic factor-1 (NR5A1) SGPP – S1P… …steroidogenic factor 1 (SF-1) is the primary transcriptional regulator of most steroidogenic… …Steroidogenic Factor 1 (SF-1): a key transcription factor in adrenal steroidogenesis The… …the role of ligands in controlling steroidogenic factor 1 (SF-1) transactivation… …insulin-like growth factor 1 IL-1β – interleukin 1 β INF-γ – interferon γ JKN – c-Jun N-terminal…

11 more images…

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lucki, N. C. (2011). Characterization of the role of acid ceramidase in adrenocortical steroid hormone biosynthesis. (Doctoral Dissertation). Georgia Tech. Retrieved from http://hdl.handle.net/1853/42804

Chicago Manual of Style (16^th Edition):

Lucki, Natasha Chrystman. “Characterization of the role of acid ceramidase in adrenocortical steroid hormone biosynthesis.” 2011. Doctoral Dissertation, Georgia Tech. Accessed January 18, 2021. http://hdl.handle.net/1853/42804.

MLA Handbook (7^th Edition):

Lucki, Natasha Chrystman. “Characterization of the role of acid ceramidase in adrenocortical steroid hormone biosynthesis.” 2011. Web. 18 Jan 2021.

Vancouver:

Lucki NC. Characterization of the role of acid ceramidase in adrenocortical steroid hormone biosynthesis. [Internet] [Doctoral dissertation]. Georgia Tech; 2011. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/1853/42804.

Council of Science Editors:

Lucki NC. Characterization of the role of acid ceramidase in adrenocortical steroid hormone biosynthesis. [Doctoral Dissertation]. Georgia Tech; 2011. Available from: http://hdl.handle.net/1853/42804

		in
And / Or
		in
And / Or
		in
And / Or
		in

Open Access Theses and Dissertations