subject:(Spr)

1. Wijaya, Edy. Design and optimization of Surface Plasmon Resonance (SPR) biosensors : Conception et optimisation des biocapteurs à base de résonance plasmonique de surface.

Degree: Docteur es, Micro et Nanotechnologies, Acoustique et Télécommunications, 2012, Université Lille I – Sciences et Technologies

URL: http://www.theses.fr/2012LIL10096

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En terme de performance, le biocapteur idéal doit avoir très grande sensibilité, basse limite de détection et temps d’analyse qui est extrêmement court. Les biocapteurs… (more)

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En terme de performance, le biocapteur idéal doit avoir très grande sensibilité, basse limite de détection et temps d’analyse qui est extrêmement court. Les biocapteurs sans marquage à base de résonance de plasmons de surface (biocapteurs SPR) possèdent naturellement le temps d’analyse le plus court parmi différent types de biocapteurs. Leur limite de détection n’est cependant pas la plus impressionnante. Il y a donc un besoin pour augmenter considérablement la sensibilité intrinsèque des biocapteurs SPR afin de permettre de plus basses limites de détection. Quelques approches pour exalter la sensibilité optique des biocapteurs SPR dans la configuration « traditionnel » de Krestchmann telles que film SPR bimétallique, plasmons à longues portées et détection dans l’infrarouge proche sont examinées dans ce travail. Des configurations « non traditionnelles » comme guides optiques planaires avec couplage par réseau et structures sub-longueur d’ondes ont été aussi théoriquement étudiées. Nouvelle stratégie de fonctionnalisation de surface à base de graphène qui augmente la sensibilité de reconnaissance biomoléculaire et peut être appliquée à quasiment toute structure SPR a été également démontrée.

In terms of performance, the ideal biosensor should have high sensitivity, low limits of detection, and extremely short analysis time. Label-free surface plasmon resonance (SPR) biosensors naturally offer the shortest analysis time compared to other types of biosensors. On the other hand, the limits of detection of SPR biosensors are not the most impressive. The inherent sensitivity of SPR biosensors thus needs to be significantly improved to allow lower limits of detection. Several approaches for the enhancement of optical sensitivity of SPR biosensors in the “traditional” attenuated total reflection (ATR) Kretschmann configuration such as the use of bimetallic SPR film, long-range surface plasmons, and near-infrared operating wavelength have been investigated in this work. In addition, some “non traditional” configurations for SPR biosensors including grating-coupled planar optical waveguides and arrays of sub-wavelength structures have been theoretically studied. Novel graphene-based surface functionalization strategy with enhanced biorecognition sensitivity that can be applied to virtually any SPR structure has also been demonstrated.

Advisors/Committee Members: Vilcot, Jean-Pierre (thesis director).

Subjects/Keywords: Spr; 681.757

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APA (6^th Edition):

Wijaya, E. (2012). Design and optimization of Surface Plasmon Resonance (SPR) biosensors : Conception et optimisation des biocapteurs à base de résonance plasmonique de surface. (Doctoral Dissertation). Université Lille I – Sciences et Technologies. Retrieved from http://www.theses.fr/2012LIL10096

Chicago Manual of Style (16^th Edition):

Wijaya, Edy. “Design and optimization of Surface Plasmon Resonance (SPR) biosensors : Conception et optimisation des biocapteurs à base de résonance plasmonique de surface.” 2012. Doctoral Dissertation, Université Lille I – Sciences et Technologies. Accessed January 18, 2021. http://www.theses.fr/2012LIL10096.

MLA Handbook (7^th Edition):

Wijaya, Edy. “Design and optimization of Surface Plasmon Resonance (SPR) biosensors : Conception et optimisation des biocapteurs à base de résonance plasmonique de surface.” 2012. Web. 18 Jan 2021.

Vancouver:

Wijaya E. Design and optimization of Surface Plasmon Resonance (SPR) biosensors : Conception et optimisation des biocapteurs à base de résonance plasmonique de surface. [Internet] [Doctoral dissertation]. Université Lille I – Sciences et Technologies; 2012. [cited 2021 Jan 18]. Available from: http://www.theses.fr/2012LIL10096.

Council of Science Editors:

Wijaya E. Design and optimization of Surface Plasmon Resonance (SPR) biosensors : Conception et optimisation des biocapteurs à base de résonance plasmonique de surface. [Doctoral Dissertation]. Université Lille I – Sciences et Technologies; 2012. Available from: http://www.theses.fr/2012LIL10096

University of Victoria

2. Valsecchi, Chiara. Development of Plasmonic Sensors for Leukemia Diagnosis.

Degree: Dept. of Chemistry, 2013, University of Victoria

URL: http://hdl.handle.net/1828/4559

► Plasmonic materials constitute one of the most explored platforms in the past decade for biological sensing, as they offer a wide range of advantages in… (more)

Subjects/Keywords: nanoholes; spr; biosensor

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Valsecchi, C. (2013). Development of Plasmonic Sensors for Leukemia Diagnosis. (Masters Thesis). University of Victoria. Retrieved from http://hdl.handle.net/1828/4559

Chicago Manual of Style (16^th Edition):

Valsecchi, Chiara. “Development of Plasmonic Sensors for Leukemia Diagnosis.” 2013. Masters Thesis, University of Victoria. Accessed January 18, 2021. http://hdl.handle.net/1828/4559.

MLA Handbook (7^th Edition):

Valsecchi, Chiara. “Development of Plasmonic Sensors for Leukemia Diagnosis.” 2013. Web. 18 Jan 2021.

Vancouver:

Valsecchi C. Development of Plasmonic Sensors for Leukemia Diagnosis. [Internet] [Masters thesis]. University of Victoria; 2013. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/1828/4559.

Council of Science Editors:

Valsecchi C. Development of Plasmonic Sensors for Leukemia Diagnosis. [Masters Thesis]. University of Victoria; 2013. Available from: http://hdl.handle.net/1828/4559

Tampereen ammattikorkeakoulu

3. Aalto, Pauliina. SPR:n tarjoaman koulutuksen merkitys sairaanhoitajalle ennen katastrofityöhön lähtöä.

Degree: 2017, Tampereen ammattikorkeakoulu

URL: http://www.theseus.fi/handle/10024/126208

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Opinnäytetyö toteutettiin yhteistyössä Suomen Punaisen Ristin kanssa. Opinnäytetyön tarkoituksena oli kuvata sitä, millaisen koulutuksen Suomen Punaisen Ristin palveluksessa olevat sairaanhoitajat saavat ennen katastrofialueelle lähtöä. Lisäksi… (more)

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Opinnäytetyö toteutettiin yhteistyössä Suomen Punaisen Ristin kanssa. Opinnäytetyön tarkoituksena oli kuvata sitä, millaisen koulutuksen Suomen Punaisen Ristin palveluksessa olevat sairaanhoitajat saavat ennen katastrofialueelle lähtöä. Lisäksi opinnäytetyössä selvitettiin sairaanhoitajien kokemuksia heidän saamastaan koulutuksesta ja sen merkityksestä katastrofityöskentelyssä. Tavoitteena oli kerätä tietoa siitä, millaisena sairaanhoitajat kokivat Suomen Punaisen Ristin koulutustilaisuudet ja koulutukset sekä järjestön toiminnan. Opinnäytetyö toteutettiin kvalitatiivisella tutkimusmenetelmällä. Aineistonkeruumenetelmänä käytettiin teemahaastattelua. Haastatteluun osallistui neljä Suomen Punaisen Ristin delegaattina työskentelevää sairaanhoitajaa. Opinnäytetyössä käytettiin aineistolähtöistä sisällönanalyysiä. Opinnäytetyön tulokset osoittivat, että sairaanhoitajat suorittivat Punaisen Ristin peruskoulutukset ja jatkokoulutuksilla erikoistumisen delegaatiksi työskentelyä varten. Pohjakoulutusten jälkeen delegaatit lisäsivät osaamistaan kouluttautumalla erilaisin kurssein. Tuloksista kävi ilmi, että sairaanhoitajat kokivat koulutukset laadukkaina, monipuolisina sekä tarpeisiin vastaavina. Tulosten mukaan delegaattien suorittamilla koulutuksilla oli keskeinen merkitys katastrofityöskentelyssä. Lisäksi tuloksista nousi esiin delegaatin sairaanhoitajan työssä hankkiman työkokemuksen merkitys katastrofityöskentelyssä. Tulosten mukaan koulutukset tarjosivat delegaateille konkreettista tietoa sekä toimintatapoja katastrofityön tekemiseen. Suomen Punainen Risti tarjoaa laadukkaita ja delegaattien tarpeita monipuolisesti huomioon ottavia koulutuksia. Opinnäytetyön tuloksia Suomen Punainen Risti voi hyödyntää toimintansa kehittämisessä. Kehittämisehdotuksia Suomen Punaiselle Ristille ovat yksinhuoltajavanhemman tukeminen delegaatin työssä, delegaatin urasuunnittelun parantaminen humanitaarisen työn puolella, koulutusten aikataulutuksen kehittäminen ja paljon tietoa sisältävän koulutuksen jakaminen useampaan koulutukseen.

This study was carried out in cooperation with the Finnish Red Cross. The purpose of this study was to describe what kind of education nurses working for the organisation are given before going to work in a disaster area. This study also charted out the experiences of nurses about the education they were given and its significance in disaster work. The objective of this study was to gather information about how the nurses experienced the received education, courses and their contents and how they saw the actions of the Finnish Red Cross as an organisation. This study was carried out using a qualitative method. The data were collected by thematic interviews of four nurses working as Red Cross delegates. The data were analysed using inductive content analysis. The results suggest that the nurses completed the basic training organised by the Red Cross and specialised by special educations for working as a delegate. These findings indicate that the nurses found the education to be high quality,…

Advisors/Committee Members: Tampereen ammattikorkeakoulu.

Subjects/Keywords: Punainen Risti; SPR

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APA (6^th Edition):

Aalto, P. (2017). SPR:n tarjoaman koulutuksen merkitys sairaanhoitajalle ennen katastrofityöhön lähtöä. (Thesis). Tampereen ammattikorkeakoulu. Retrieved from http://www.theseus.fi/handle/10024/126208

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Aalto, Pauliina. “SPR:n tarjoaman koulutuksen merkitys sairaanhoitajalle ennen katastrofityöhön lähtöä.” 2017. Thesis, Tampereen ammattikorkeakoulu. Accessed January 18, 2021. http://www.theseus.fi/handle/10024/126208.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Aalto, Pauliina. “SPR:n tarjoaman koulutuksen merkitys sairaanhoitajalle ennen katastrofityöhön lähtöä.” 2017. Web. 18 Jan 2021.

Vancouver:

Aalto P. SPR:n tarjoaman koulutuksen merkitys sairaanhoitajalle ennen katastrofityöhön lähtöä. [Internet] [Thesis]. Tampereen ammattikorkeakoulu; 2017. [cited 2021 Jan 18]. Available from: http://www.theseus.fi/handle/10024/126208.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Aalto P. SPR:n tarjoaman koulutuksen merkitys sairaanhoitajalle ennen katastrofityöhön lähtöä. [Thesis]. Tampereen ammattikorkeakoulu; 2017. Available from: http://www.theseus.fi/handle/10024/126208

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Alberta

4. Toman, John T. Electrografted Thick Diazonium Derived Films for Biosensing Applications.

Degree: MS, Department of Chemistry, 2013, University of Alberta

URL: https://era.library.ualberta.ca/files/cf95jb56k

► Electrografting permits bonding of an organic film to a conductive substrate. It is therefore important to control the formation of organic films as best as… (more)

Subjects/Keywords: Biosensing; Diazonium; SPR; Electrografting

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APA (6^th Edition):

Toman, J. T. (2013). Electrografted Thick Diazonium Derived Films for Biosensing Applications. (Masters Thesis). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/cf95jb56k

Chicago Manual of Style (16^th Edition):

Toman, John T. “Electrografted Thick Diazonium Derived Films for Biosensing Applications.” 2013. Masters Thesis, University of Alberta. Accessed January 18, 2021. https://era.library.ualberta.ca/files/cf95jb56k.

MLA Handbook (7^th Edition):

Toman, John T. “Electrografted Thick Diazonium Derived Films for Biosensing Applications.” 2013. Web. 18 Jan 2021.

Vancouver:

Toman JT. Electrografted Thick Diazonium Derived Films for Biosensing Applications. [Internet] [Masters thesis]. University of Alberta; 2013. [cited 2021 Jan 18]. Available from: https://era.library.ualberta.ca/files/cf95jb56k.

Council of Science Editors:

Toman JT. Electrografted Thick Diazonium Derived Films for Biosensing Applications. [Masters Thesis]. University of Alberta; 2013. Available from: https://era.library.ualberta.ca/files/cf95jb56k

5. Okeke, Joseph Chukwuemeka; Isienyi, Osita Kingsley. Analysis of Strength of Self -Pierce Riveted Aluminium Plate Using Finite Element Method.

Degree: 2008, , Department of Mechanical Engineering

URL: http://urn.kb.se/resolve?urn=urn:nbn:se:bth-3834

► This work aims at providing a numerical tool for the efficient design of the self piercing rivet by means of finite element simulations. Abaqus… (more)

Subjects/Keywords: spr rivet

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APA (6^th Edition):

Okeke, Joseph Chukwuemeka; Isienyi, O. K. (2008). Analysis of Strength of Self -Pierce Riveted Aluminium Plate Using Finite Element Method. (Thesis). , Department of Mechanical Engineering. Retrieved from http://urn.kb.se/resolve?urn=urn:nbn:se:bth-3834

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Okeke, Joseph Chukwuemeka; Isienyi, Osita Kingsley. “Analysis of Strength of Self -Pierce Riveted Aluminium Plate Using Finite Element Method.” 2008. Thesis, , Department of Mechanical Engineering. Accessed January 18, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:bth-3834.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Okeke, Joseph Chukwuemeka; Isienyi, Osita Kingsley. “Analysis of Strength of Self -Pierce Riveted Aluminium Plate Using Finite Element Method.” 2008. Web. 18 Jan 2021.

Vancouver:

Okeke, Joseph Chukwuemeka; Isienyi OK. Analysis of Strength of Self -Pierce Riveted Aluminium Plate Using Finite Element Method. [Internet] [Thesis]. , Department of Mechanical Engineering; 2008. [cited 2021 Jan 18]. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:bth-3834.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Okeke, Joseph Chukwuemeka; Isienyi OK. Analysis of Strength of Self -Pierce Riveted Aluminium Plate Using Finite Element Method. [Thesis]. , Department of Mechanical Engineering; 2008. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:bth-3834

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Victoria

6. Yu, Ting. Detection of biomarkers for lung cancer and leukemia using SPR nanohole-based sensors.

Degree: Dept. of Chemistry, 2013, University of Victoria

URL: http://hdl.handle.net/1828/4661

► Cancer is a leading cause of death and some types of cancer are hard to diagnose at early stages. An accurate method for subtype classification… (more)

Subjects/Keywords: SPR sensor; Cancer; Biomarker

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Yu, T. (2013). Detection of biomarkers for lung cancer and leukemia using SPR nanohole-based sensors. (Masters Thesis). University of Victoria. Retrieved from http://hdl.handle.net/1828/4661

Chicago Manual of Style (16^th Edition):

Yu, Ting. “Detection of biomarkers for lung cancer and leukemia using SPR nanohole-based sensors.” 2013. Masters Thesis, University of Victoria. Accessed January 18, 2021. http://hdl.handle.net/1828/4661.

MLA Handbook (7^th Edition):

Yu, Ting. “Detection of biomarkers for lung cancer and leukemia using SPR nanohole-based sensors.” 2013. Web. 18 Jan 2021.

Vancouver:

Yu T. Detection of biomarkers for lung cancer and leukemia using SPR nanohole-based sensors. [Internet] [Masters thesis]. University of Victoria; 2013. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/1828/4661.

Council of Science Editors:

Yu T. Detection of biomarkers for lung cancer and leukemia using SPR nanohole-based sensors. [Masters Thesis]. University of Victoria; 2013. Available from: http://hdl.handle.net/1828/4661

University of Notre Dame

7. Chinmoy Nath. Theoretical and Laboratory Modeling of Turbulent Jets in Low Aspect Ratio Cavities</h1>.

Degree: Civil Engineering and Geological Sciences, 2014, University of Notre Dame

URL: https://curate.nd.edu/show/s1784j05s99

► This research is motivated by the need to understand and parameterize mixing processes that occur during degassing and refilling of oil in large underground… (more)

▼ This research is motivated by the need to understand and parameterize mixing processes that occur during degassing and refilling of oil in large underground strategic petroleum reserve SPR caverns. The oil is pumped from cavern bottom to a degassing station and then returned to the cavern by using a vertical turbulent jet located near the cavern top. This dissertation concerns a laboratory experimental and theoretical modeling program conducted to investigate: (a) mixing mechanisms and precession of turbulent jets in homogeneous fluids (b) mixing of turbulent jet in stratified fluids and © wall attachment of offset jet in a homogeneous fluid. In the first part, a round turbulent axisymmetric jet is discharged in a long cylinder. It is found that the flow does not reach a true steady state, but vacillates periodically. Digital video recordings and particle image velocimetry are used to map the flow structures and velocity/vorticity fields, from which the frequency of jet switching, jet stopping distance, mean flow, turbulence characteristics and the influence of end-wall boundary conditions are inferred. The results are parameterized using the characteristic length D and velocity J^ (½)/D scales based on the jet kinematic momentum flux J and cylinder width D. The scaling laws so developed could be used to extrapolate laboratory observations to SPR flows. In the second part, a turbulent (positively or negatively) buoyant jet is injected vertically into a slender cylinder containing a stratified fluid. The interest is the vertical density distribution in the container and its dependence on time and other parameters. For each case (lighter or heavier jet) the experimental data, when properly non-dimensionalized, could be collapsed into a ‘universal’ time dependent behavior. A theoretical model is advanced to explain the results. Finally, a round turbulent offset jet in a low-aspect ratio cylinder is investigated. Particle Image Velocimetry and flow visualization are used for flow diagnostics. The measurements include the jet penetration (mixing) depth l, jet spreading rate and the mean velocity/vorticity fields for different offset positions ‘Delta’. With the introduction of offset, the flow patterns change drastically. For 0 < Delta/D < 0.2, the jet deflects toward the wall while precessing (as in the axisymmetric case), for 0.2 < Delta/D < 0.4 the jet hugs the wall but with an oscillating tail, and for 0.45 < Delta/D the jet appears as a wall jet. In all cases, the jet is destroyed at a certain distance (mixing or penetration depth) from the origin. This mixing depth takes its lowest value for 0 < Delta/D < 0.2, with l ≈ (3.2-3.6)D, becomes maximum at Delta/D equal to 0.4 with l ≈ 5.2D, and drops to l ≈ 4.5D when the jet is close to the wall. Based on derived results some recommendations are made for optimal operation of SPR degassing. Advisors/Committee Members: Sergey I. Voropayev, Committee Member, Andrew Kennedy, Committee Member, Harindra Joseph Fernando, Committee Chair, Joannes J. Westerink, Committee Member.

Subjects/Keywords: Turbulence; SPR Oil Caverns; Jets

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Nath, C. (2014). Theoretical and Laboratory Modeling of Turbulent Jets in Low Aspect Ratio Cavities</h1>. (Thesis). University of Notre Dame. Retrieved from https://curate.nd.edu/show/s1784j05s99

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Nath, Chinmoy. “Theoretical and Laboratory Modeling of Turbulent Jets in Low Aspect Ratio Cavities</h1>.” 2014. Thesis, University of Notre Dame. Accessed January 18, 2021. https://curate.nd.edu/show/s1784j05s99.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Nath, Chinmoy. “Theoretical and Laboratory Modeling of Turbulent Jets in Low Aspect Ratio Cavities</h1>.” 2014. Web. 18 Jan 2021.

Vancouver:

Nath C. Theoretical and Laboratory Modeling of Turbulent Jets in Low Aspect Ratio Cavities</h1>. [Internet] [Thesis]. University of Notre Dame; 2014. [cited 2021 Jan 18]. Available from: https://curate.nd.edu/show/s1784j05s99.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Nath C. Theoretical and Laboratory Modeling of Turbulent Jets in Low Aspect Ratio Cavities</h1>. [Thesis]. University of Notre Dame; 2014. Available from: https://curate.nd.edu/show/s1784j05s99

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidad de Navarra

8. [No author]. Desarrollo, optimización e implementación de un protocolo biológico de reconocimiento del Factor de Necrosis Tumoral alfa (TNFα) mediante técnicas de resonancia plasmónica superficial.

Degree: 2014, Universidad de Navarra

URL: http://hdl.handle.net/10171/35976

► El TNFα es una citoquina proinflamatoria con diversas funciones en el organismo. Está implicada en múltiples acciones fisiológicas como la hematopoyesis, el sistema inmune y… (more)

▼ El TNFα es una citoquina proinflamatoria con diversas funciones en el organismo. Está implicada en múltiples acciones fisiológicas como la hematopoyesis, el sistema inmune y la regresión tumoral. Debido a su importancia, cuando algo falla en su regulación o funcionamiento, tiene graves y diversas consecuencias patológicas. Por ello, es la molécula diana de muchos tratamientos efectivos para enfermedades inflamatorias como el síndrome de Crohn, la aterosclerosis la artritis reumatoide. Sin embargo, a día de hoy no se realiza de manera rutinaria su determinación para el diagnóstico en análisis clínico. Ni siquiera se lleva a cabo el seguimiento de los niveles de esta molécula en pacientes tratados con fármacos terapéuticos anti TNFα. Las únicas técnicas actuales para medir su concentración son la ELISA y las técnicas basadas en ella sólo se utilizan en estudios de investigación debido a su elevado coste. Por un lado, este trabajo ha pretendido impulsar el conocimiento sobre el TNFα mediante una revisión exhaustiva de la bibliografía recogida hasta ahora en artículos y libros científicos. Por otro lado, se ha caracterizado su detección diseñando un protocolo para la formación de una estructura de reconocimiento biológico de la citoquina con el uso de las SAMs mediante técnicas de resonancia plasmónica superficial (SPR). Para ello, se han optimizado todos los parámetros del procedimiento paso por paso. Se ha comenzado con el tipo de SAM, concentraciones y técnicas de formación estudiadas. Después, se ha analizado la adición de distintos bloqueantes de oro y desactivadores de SAM al proceso con el fin de minimizar las uniones inespecíficas. Se ha continuado con el estudio de las condiciones óptimas para la activación de la SAM y la inmovilización del ligando para poder detectar el máximo de analito posible, probando distintas concentraciones de TNFα con las condiciones óptimas obtenidas. Por último, utilizando la estructura de biorreconocimiento de TNFα óptima, se han analizado diferentes muestras de plasma de ratas sanas y con esteatosis inducida proporcionadas por el Hospital Donostia, pudiendo distinguir entre las que tenían concentraciones basales y elevadas de TNFα en plasma con el sistema diseñado. Además, se ha caracterizado cada paso optimizado con técnicas de diversa índole. Este proyecto ha permitido profundizar en el conocimiento del TNFα, los biosensores, las técnicas libres de marcaje y las monocapas autoensambladas, además de la optimización del protocolo de obtención de una estructura de biorreconocimiento para la detección de TNFα mediante técnicas de resonancia plasmónica superficial, obteniendo el diseño y producción de una SAM efectiva para este caso. Advisors/Committee Members: Arana Alonso, Sergio (advisor), Mujica-Garmendia, M. (Maite) (advisor).

Subjects/Keywords: Inflamación.; TNFα.; Detección.; SPR.; SAM.

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APA (6^th Edition):

author], [. (2014). Desarrollo, optimización e implementación de un protocolo biológico de reconocimiento del Factor de Necrosis Tumoral alfa (TNFα) mediante técnicas de resonancia plasmónica superficial. (Thesis). Universidad de Navarra. Retrieved from http://hdl.handle.net/10171/35976

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

author], [No. “Desarrollo, optimización e implementación de un protocolo biológico de reconocimiento del Factor de Necrosis Tumoral alfa (TNFα) mediante técnicas de resonancia plasmónica superficial. ” 2014. Thesis, Universidad de Navarra. Accessed January 18, 2021. http://hdl.handle.net/10171/35976.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

author], [No. “Desarrollo, optimización e implementación de un protocolo biológico de reconocimiento del Factor de Necrosis Tumoral alfa (TNFα) mediante técnicas de resonancia plasmónica superficial. ” 2014. Web. 18 Jan 2021.

Vancouver:

author] [. Desarrollo, optimización e implementación de un protocolo biológico de reconocimiento del Factor de Necrosis Tumoral alfa (TNFα) mediante técnicas de resonancia plasmónica superficial. [Internet] [Thesis]. Universidad de Navarra; 2014. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/10171/35976.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

author] [. Desarrollo, optimización e implementación de un protocolo biológico de reconocimiento del Factor de Necrosis Tumoral alfa (TNFα) mediante técnicas de resonancia plasmónica superficial. [Thesis]. Universidad de Navarra; 2014. Available from: http://hdl.handle.net/10171/35976

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Université de Grenoble

9. Daniel, Camille. Biopuce à aptamères anti-thrombine : exploration d'une technique alternative de détection : Aptamer biochip : Exploration of an alternative detection technique.

Degree: Docteur es, Physique, 2013, Université de Grenoble

URL: http://www.theses.fr/2013GRENY037

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Du fait de leur haute stabilité et bas coût de production, les aptamères suscitent un intérêt croissant, depuis près de 20 ans, dans le design… (more)

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Du fait de leur haute stabilité et bas coût de production, les aptamères suscitent un intérêt croissant, depuis près de 20 ans, dans le design de biocapteurs en tant qu'élément de reconnaissance idéal. Le but de ce travail de thèse est de démontrer l'intérêt et la pertinence d'un outil tel qu'une biopuce à aptamères, associant les avantages des sondes aptamères à ceux d'une détection par SPRi (Surface Plasmon Resonance imaging), permettant une détection sans marquage et en temps réel d'interactions moléculaires. Dans ce but, deux aptamères anti-thrombine (APT1 = 5′- GGT-TGG-TGT-GGT-TGG -3′ et APT2 = 5′-AGT-CCG-TGG-TAG-GGG-AGG-TTG-GGG-TGA-CT-3′) ont été choisis comme objets d'étude modèles. Ce choix a permis d'orienter différents axes de recherche : utilisés indépendamment comme sondes lors de l'élaboration de notre biopuce, ils ont tout d'abord permis de réaliser une détection cinétique optimisée de la thrombine, avec des performances remarquables pour une détection de ce type, ainsi que le calcul de constantes de dissociation en solution et à la surface des biopuces. Mais au-delà d'un simple biocapteur, la biopuce a également pu être utilisée comme véritable plateforme d'étude de la thrombine et de ses interactions, au sein de structures plus complexes telles que la structure « sandwich » entre les deux aptamères, ou d'autres interactions impliquant la thrombine en tant qu'acteur de la cascade de coagulation (inhibition de la thrombine par l'antithrombine III et le cofacteur II de l'héparine, transformation de la prothrombine au sein du complexe prothrombinase).

For 20 years, aptamers have been raising an increasing interest for biosensor applications as replacements for antibodies, due to their high stability and low cost. The main objective of this Ph.D. thesis is to show the great capacities of an aptamer biochip that combines the advantages of aptamer probes associated with a SPRi (Surface Plasomn Resonance imaging) detection to monitor, in real-time and in a label-free manner, molecular interactions occurring on the surface of the biochip. Two aptamers selected against the thrombin protein (APT1 = 5′- GGT-TGG-TGT-GGT-TGG -3′ and APT2 = 5′-AGT-CCG-TGG-TAG-GGG-AGG-TTG-GGG-TGA-CT-3′) were chosen as models for our study. This choice led to the exploration of different lines of research. First, both aptamers were used independently to develop a kinetic biosensor with remarkable performances for the quantification of thrombin. This tool served to determine independently, and compare, both the solution- and surface-phase affinities of the trombin-APT2 interaction. But more than a simple and effective biosensor, this kind of biochip represents a true platform to study the protein and its interactions within complex structures, such as the sandwich-like architecture with APT1 and APT2, or its interactions with other factors of the coagulation cascade (inhibition of thrombin by antithrombin III and heparin cofactor II, conversion of prothrombin into thrombin by the prothrombinase complex).

Advisors/Committee Members: Buhot, Arnaud (thesis director), Roupioz, Yoann (thesis director).

Subjects/Keywords: Aptamère; Biopuce; Thrombine; SPR; Aptamer; Biochip; Thrombin; SPR

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Daniel, C. (2013). Biopuce à aptamères anti-thrombine : exploration d'une technique alternative de détection : Aptamer biochip : Exploration of an alternative detection technique. (Doctoral Dissertation). Université de Grenoble. Retrieved from http://www.theses.fr/2013GRENY037

Chicago Manual of Style (16^th Edition):

Daniel, Camille. “Biopuce à aptamères anti-thrombine : exploration d'une technique alternative de détection : Aptamer biochip : Exploration of an alternative detection technique.” 2013. Doctoral Dissertation, Université de Grenoble. Accessed January 18, 2021. http://www.theses.fr/2013GRENY037.

MLA Handbook (7^th Edition):

Daniel, Camille. “Biopuce à aptamères anti-thrombine : exploration d'une technique alternative de détection : Aptamer biochip : Exploration of an alternative detection technique.” 2013. Web. 18 Jan 2021.

Vancouver:

Daniel C. Biopuce à aptamères anti-thrombine : exploration d'une technique alternative de détection : Aptamer biochip : Exploration of an alternative detection technique. [Internet] [Doctoral dissertation]. Université de Grenoble; 2013. [cited 2021 Jan 18]. Available from: http://www.theses.fr/2013GRENY037.

Council of Science Editors:

Daniel C. Biopuce à aptamères anti-thrombine : exploration d'une technique alternative de détection : Aptamer biochip : Exploration of an alternative detection technique. [Doctoral Dissertation]. Université de Grenoble; 2013. Available from: http://www.theses.fr/2013GRENY037

University of Alberta

10. Cao, Cong. Investigating Interactions between Cellulose Nanocrystals (CNCs) and Proteins.

Degree: MS, Department of Chemistry, 2013, University of Alberta

URL: https://era.library.ualberta.ca/files/m613mz063

► Cellulose nanocrystals (CNCs) are an emerging renewable and sustainable nanomaterial which has received increasing attention. They are abundant, nontoxic, biodegradable and chemically inert. CNCs have… (more)

Subjects/Keywords: SPR; Cellulose Nanocrystals; AFM; Protein; Interaction; IRRAS

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APA (6^th Edition):

Cao, C. (2013). Investigating Interactions between Cellulose Nanocrystals (CNCs) and Proteins. (Masters Thesis). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/m613mz063

Chicago Manual of Style (16^th Edition):

Cao, Cong. “Investigating Interactions between Cellulose Nanocrystals (CNCs) and Proteins.” 2013. Masters Thesis, University of Alberta. Accessed January 18, 2021. https://era.library.ualberta.ca/files/m613mz063.

MLA Handbook (7^th Edition):

Cao, Cong. “Investigating Interactions between Cellulose Nanocrystals (CNCs) and Proteins.” 2013. Web. 18 Jan 2021.

Vancouver:

Cao C. Investigating Interactions between Cellulose Nanocrystals (CNCs) and Proteins. [Internet] [Masters thesis]. University of Alberta; 2013. [cited 2021 Jan 18]. Available from: https://era.library.ualberta.ca/files/m613mz063.

Council of Science Editors:

Cao C. Investigating Interactions between Cellulose Nanocrystals (CNCs) and Proteins. [Masters Thesis]. University of Alberta; 2013. Available from: https://era.library.ualberta.ca/files/m613mz063

Victoria University of Wellington

11. Dobhal, Garima. Quantum dot bioconjugates for the detection of extracellular vesicles in saliva and breath.

Degree: 2019, Victoria University of Wellington

URL: http://hdl.handle.net/10063/8119

► Nano-sized extracellular vesicles, released by most types of cells, contain information about the cell they originate from and have been shown to be involved in… (more)

▼ Nano-sized extracellular vesicles, released by most types of cells, contain information about the cell they originate from and have been shown to be involved in a variety of cellular processes. However, their detection and characterisation has been challenging and non-standardised, which makes comparisons across literature very challenging. While exosomes are known to exist in complex biological fluids such as saliva, breast milk, blood, and urine, their separation and identification from these media are time-consuming. Many researchers use techniques such as transmission electron microscopy for physical characterisation and western blot for protein identification, which are often not available in medical settings. Additionally, while these fluids can be easily obtained, acquiring similar samples from lung environments is a highly invasive procedure. While breath is known to transmit droplets from the lungs, the presence of exosomes in these condensates is unknown. In this project, functionalised InP/ZnS quantum dots (QDs) were used to target exosomes from a number of biological sources and provide a gateway to more fully characterise their ensemble properties. The InP/ZnS QDs were synthesised, and their size dependency on the band gap was investigated in accordance with the theoretical effective mass approximation model for quantum dots. The QDs were produced with hydrophobic oleylamine ligands, and therefore had to be ligand exchanged to be used in biological applications. A range of ligand exchange methods was surveyed to probe the best balance between retention of original quantum yields and best colloidal stability in aqueous systems.The QDs were further conjugated to an antibody specific for CD63, the protein found on exosomes. The conjugation was confirmed using dynamic light scattering and surface plasmon resonance. Finally, the binding of the QD-Antibody probe to the exosome was confirmed using surface plasmon resonance and confocal microscopy. Further modifications of the assay system could lead to multiplex-detection of the different proteins on the exosomes, their characterisation, and a method for the rapid detection of diseases. Advisors/Committee Members: Goreham, Renee, Nann, Thomas.

Subjects/Keywords: Exosomes; InP/ZnS quantum dots; Detection; SPR

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APA (6^th Edition):

Dobhal, G. (2019). Quantum dot bioconjugates for the detection of extracellular vesicles in saliva and breath. (Masters Thesis). Victoria University of Wellington. Retrieved from http://hdl.handle.net/10063/8119

Chicago Manual of Style (16^th Edition):

Dobhal, Garima. “Quantum dot bioconjugates for the detection of extracellular vesicles in saliva and breath.” 2019. Masters Thesis, Victoria University of Wellington. Accessed January 18, 2021. http://hdl.handle.net/10063/8119.

MLA Handbook (7^th Edition):

Dobhal, Garima. “Quantum dot bioconjugates for the detection of extracellular vesicles in saliva and breath.” 2019. Web. 18 Jan 2021.

Vancouver:

Dobhal G. Quantum dot bioconjugates for the detection of extracellular vesicles in saliva and breath. [Internet] [Masters thesis]. Victoria University of Wellington; 2019. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/10063/8119.

Council of Science Editors:

Dobhal G. Quantum dot bioconjugates for the detection of extracellular vesicles in saliva and breath. [Masters Thesis]. Victoria University of Wellington; 2019. Available from: http://hdl.handle.net/10063/8119

Université de Sherbrooke

12. Maltais, Jean-Sébastien. RAGE comme nouvelle cible thérapeutique prévenant le stress du réticulum endoplasmique et l’apoptose des cellules du muscle lisse vasculaire associés avec le diabète: RAGE as a novel therapeutic target to prevent reticulum endoplasmic stress and apoptosis in vascular smooth muscle cells associated with diabetes.

Degree: 2016, Université de Sherbrooke

URL: http://hdl.handle.net/11143/8804

► Abstract : Cardiovascular diseases represent, to a large extent, the first cause of morbidity and mortality among people with diabetes. RAGE activation by advanced glycation… (more)

▼ Abstract : Cardiovascular diseases represent, to a large extent, the first cause of morbidity and mortality among people with diabetes. RAGE activation by advanced glycation end products (AGE) generated in hyperglycemic conditions is associated to a multitude of vascular diabetic complications, in particular by a signaling promoting chronic inflammation as well as death of cells forming tissues and organs exposed to AGE. Overexpression of RAGE in smooth muscle cells of vulnerable atheromatous plaques suggests the receptor could contribute to heart attacks and strokes. Therefore, we hypothesize that RAGE activation in smooth muscle cells is involved in apoptosis. To verify this hypothesis, we first designed a new label-free assay based of surface plasmon resonance (SPR) to measure apoptosis of a cell monolayer in real-time and to characterize precisely the kinetic parameters of the initiation and execution phases. This assay showed that RAGE activation induces apoptosis of more than 75.6% of smooth muscle cells stimulated with CML-HSA for 20 hours. Moreover, we noticed that RAGE activation generated strong endoplasmic reticular stress, indicated by the formation of a great number of stress granules as well as the increased expression of stress marker HuR and caspase-9, two important regulators of reticular stress-induced apoptosis. In order, to assess the potential of an antagonist to block RAGE activation, we then synthesized the iRAGE peptide whose sequence is derived from a binding site of CML-HSA that has the particularity of owning numerous negative charges at physiological pH. Pretreatment with iRAGE was successful to prevent activation of NF-κB, induction of apoptosis and generation of endoplasmic reticular stress. We suggest a model by which iRAGE inhibits RAGE signaling by hindering the binding of multimeric ligands and by stabilizing the receptors in a monomer state. Ultimately, the synthesis of a RAGE antagonist usable in clinic could constitute a major progress in the prevention of vascular complications and in the quality of life of people with diabetes. Advisors/Committee Members: Grandbois, Michel (advisor), Gendron, Louis (advisor).

Subjects/Keywords: SPR; RAGE; AGE; Apoptose; Diabète; Apoptosis; Diabetes

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APA (6^th Edition):

Maltais, J. (2016). RAGE comme nouvelle cible thérapeutique prévenant le stress du réticulum endoplasmique et l’apoptose des cellules du muscle lisse vasculaire associés avec le diabète: RAGE as a novel therapeutic target to prevent reticulum endoplasmic stress and apoptosis in vascular smooth muscle cells associated with diabetes. (Doctoral Dissertation). Université de Sherbrooke. Retrieved from http://hdl.handle.net/11143/8804

Chicago Manual of Style (16^th Edition):

Maltais, Jean-Sébastien. “RAGE comme nouvelle cible thérapeutique prévenant le stress du réticulum endoplasmique et l’apoptose des cellules du muscle lisse vasculaire associés avec le diabète: RAGE as a novel therapeutic target to prevent reticulum endoplasmic stress and apoptosis in vascular smooth muscle cells associated with diabetes.” 2016. Doctoral Dissertation, Université de Sherbrooke. Accessed January 18, 2021. http://hdl.handle.net/11143/8804.

MLA Handbook (7^th Edition):

Maltais, Jean-Sébastien. “RAGE comme nouvelle cible thérapeutique prévenant le stress du réticulum endoplasmique et l’apoptose des cellules du muscle lisse vasculaire associés avec le diabète: RAGE as a novel therapeutic target to prevent reticulum endoplasmic stress and apoptosis in vascular smooth muscle cells associated with diabetes.” 2016. Web. 18 Jan 2021.

Vancouver:

Maltais J. RAGE comme nouvelle cible thérapeutique prévenant le stress du réticulum endoplasmique et l’apoptose des cellules du muscle lisse vasculaire associés avec le diabète: RAGE as a novel therapeutic target to prevent reticulum endoplasmic stress and apoptosis in vascular smooth muscle cells associated with diabetes. [Internet] [Doctoral dissertation]. Université de Sherbrooke; 2016. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/11143/8804.

Council of Science Editors:

Maltais J. RAGE comme nouvelle cible thérapeutique prévenant le stress du réticulum endoplasmique et l’apoptose des cellules du muscle lisse vasculaire associés avec le diabète: RAGE as a novel therapeutic target to prevent reticulum endoplasmic stress and apoptosis in vascular smooth muscle cells associated with diabetes. [Doctoral Dissertation]. Université de Sherbrooke; 2016. Available from: http://hdl.handle.net/11143/8804

University of Minnesota

13. Jordan, Luke. Measuring Binding Kinetics of Therapeutic Antibodies to Membrane Receptors Using Nanohole Array SPR Biosensors.

Degree: PhD, Biomedical Engineering, 2016, University of Minnesota

URL: http://hdl.handle.net/11299/181744

► In the field of drug discovery, two important metrics of candidate drugs are their binding affinity and kinetics to target receptors. Dr. Moses Rodriguez and… (more)

▼ In the field of drug discovery, two important metrics of candidate drugs are their binding affinity and kinetics to target receptors. Dr. Moses Rodriguez and his colleagues at the Mayo Clinic have found monoclonal IgM antibodies exhibiting therapeutic effects for multiple sclerosis and amyotrophic lateral sclerosis in animal models, and therefore desired to obtain the kinetic profiles of these antibodies to their targets. Dr. Sang-Hyun Oh’s lab at the University of Minnesota specializes in designing and fabricating plasmonic devices, and have developed a nanohole array sensor coated with silicone dioxide which permits formation of cell mimicking supported lipid bilayers. The focus of this dissertation has been to build these devices and develop assays to measure the binding between these antibodies and receptors in cell extracts and supported lipid bilayers. The first antibody to measure was rHIgM22, which binds to myelin membrane. We did not know the receptor, so we used myelin extracts which would include the unknown receptors, and attached these particles to the sensor surface by passive immobilization. To reduce particle size into the sensor detection window, we extruded the particles through pores of known dimensions. After immobilization we measured binding with antibodies. Unfortunately, binding with rHIgM22 was undetectable, but a similar antibody, mouse IgM O4, which also binds to myelin and has a therapeutic effect, did bind consistently and gave KD, apparent = 2.6 ± 3.6 nM, ka = 2.5 ± 0.0l × 10⁴ M^-1^s-1, and kd,slow = 6.6 ± 0.3 × 10^-5 s^-1. The second antibody to measure was rHIgM12, which binds to neuronal membranes. We found rHIgM12 binds to the gangliosides GT1b and GD1a, but not GM1. These gangliosides were incorporated into supported lipid bilayers (5 mol %) and binding to the antibodies was measured. Binding of rHIgM12 to GT1b gave KD, apparent = 24.8 ± 7.9 nM, ka = 2.19 ± 0.196 × 10⁴ M^-1s^-1, and kd,slow = 4.72 ± 1.15 × 10^-4 s^-1. Binding of rHIgM12 to GD1a gave KD, apparent = 42.3 ± 20.6 nM, ka = 1.79 ± 0.516 × 10⁴ M^-1s^-1, and kd,slow = 4.43 ± 1.38 × 10^-4 s^-1.

Subjects/Keywords: antibodies; kinetics; membrane; myelin; SLB; SPR

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Jordan, L. (2016). Measuring Binding Kinetics of Therapeutic Antibodies to Membrane Receptors Using Nanohole Array SPR Biosensors. (Doctoral Dissertation). University of Minnesota. Retrieved from http://hdl.handle.net/11299/181744

Chicago Manual of Style (16^th Edition):

Jordan, Luke. “Measuring Binding Kinetics of Therapeutic Antibodies to Membrane Receptors Using Nanohole Array SPR Biosensors.” 2016. Doctoral Dissertation, University of Minnesota. Accessed January 18, 2021. http://hdl.handle.net/11299/181744.

MLA Handbook (7^th Edition):

Jordan, Luke. “Measuring Binding Kinetics of Therapeutic Antibodies to Membrane Receptors Using Nanohole Array SPR Biosensors.” 2016. Web. 18 Jan 2021.

Vancouver:

Jordan L. Measuring Binding Kinetics of Therapeutic Antibodies to Membrane Receptors Using Nanohole Array SPR Biosensors. [Internet] [Doctoral dissertation]. University of Minnesota; 2016. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/11299/181744.

Council of Science Editors:

Jordan L. Measuring Binding Kinetics of Therapeutic Antibodies to Membrane Receptors Using Nanohole Array SPR Biosensors. [Doctoral Dissertation]. University of Minnesota; 2016. Available from: http://hdl.handle.net/11299/181744

Boston University

14. Kieras, Elizabeth. Functional significance of the interaction between inducible costimulator (ICOS) and its ligand (ICOSL).

Degree: 2014, Boston University

URL: http://hdl.handle.net/2144/14649

► BACKGROUND Inducible costimulator (ICOS) and its ligand (ICOSL) are a pair of costimulatory molecules that co-localize in germinal centers (GC). This interaction is critical for… (more)

▼ BACKGROUND Inducible costimulator (ICOS) and its ligand (ICOSL) are a pair of costimulatory molecules that co-localize in germinal centers (GC). This interaction is critical for the maturation of GC B cells to affinity-matured memory B cells and long-lived plasma cells. Both ICOS and ICOSL are implicated in systemic lupus erythematosus (SLE). It is known that ICOSL sheds from the cell membrane and that the soluble form of ICOSL (sICOSL) is elevated in SLE; though the function of sICOSL is poorly understood. While it is known that binding of ICOSL on antigen-presenting cells (APC) to ICOS on T cells leads to cell signaling resulting in T cell activation and differentiation, there is also some preliminary evidence that reverse signaling may also occur through ICOSL in APCs. The binding interaction between ICOS and sICOSL has not been fully characterized and is important to understand if either molecule is to be targeted therapeutically. The hypothesis evaluated in this study was that the ICOS: ICOSL interaction is a potent and critical mediator of proinflammatory signaling and immune activation that functions both via activated T cell-mediated forward signaling and APC-mediated reverse signaling mechanisms and that ectodomain shedding of ICOSL is a protective mechanism that leads to down-regulation of the proinflammatorysignaling cascade initiated by this interaction. The aim of this thesis is to characterize the binding interaction between ICOS and ICOSL and to provide a review of the literature and discuss future work that would enhance the biological understanding of this interaction and its role in lupus and other autoimmune diseases. METHODS The binding interaction between ICOS and ICOSL was characterized using both soluble proteins and cells with expressed recombinant proteins. Purified soluble ICOSL (sICOSL) was characterized using size-exclusion chromatography multiangle light scattering (SEC-MALS). Surface plasmon resonance (SPR) was used to measure the binding affinity between sICOSL and human ICOS fused to the fragment crystallizable (Fc) portion of an immunoglobulin molecule (hICOS.Fc). The binding interaction was further characterized to account for avidity between hICOS.Fc and sICOSL and between hICOS.Fc and ICOSL expressed recombinant on the cell surface using a solution-based binding method. RESULTS Expressed recombinant and purified sICOSL dimerized over time and with increasing temperatures. The sICOSL: hICOS.Fc interaction did not follow a typical 1:1 binding interaction. In-solution binding experiments resulted in a tighter equilibrium dissociation binding constant (KD) than the surface-based results obtained by SPR. The KD for hICOS.Fc binding to human ICOSL(hICOSL) expressed on cells agreed well with the KD for hICOS.Fc to the soluble protein, indicating that the in-solution binding measurement may measure binding avidity rather than affinity and that this may be the more physiologically relevant interaction. CONCLUSIONS I show in the experimental part of this study that the interaction…

Subjects/Keywords: Immunology; ICOS; ICOSL; KinExA; SPR; Costimulation; Lupus

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APA (6^th Edition):

Kieras, E. (2014). Functional significance of the interaction between inducible costimulator (ICOS) and its ligand (ICOSL). (Thesis). Boston University. Retrieved from http://hdl.handle.net/2144/14649

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kieras, Elizabeth. “Functional significance of the interaction between inducible costimulator (ICOS) and its ligand (ICOSL).” 2014. Thesis, Boston University. Accessed January 18, 2021. http://hdl.handle.net/2144/14649.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kieras, Elizabeth. “Functional significance of the interaction between inducible costimulator (ICOS) and its ligand (ICOSL).” 2014. Web. 18 Jan 2021.

Vancouver:

Kieras E. Functional significance of the interaction between inducible costimulator (ICOS) and its ligand (ICOSL). [Internet] [Thesis]. Boston University; 2014. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/2144/14649.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kieras E. Functional significance of the interaction between inducible costimulator (ICOS) and its ligand (ICOSL). [Thesis]. Boston University; 2014. Available from: http://hdl.handle.net/2144/14649

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of South Florida

15. Maignan, Jordany Richarlson. Development of Orally Bioavailable 4(1H)-Quinolones and 1,2,3,4-Tetrahydroacridin-9(10H)-ones with Potent Anti-malarial Activity.

Degree: 2015, University of South Florida

URL: https://scholarcommons.usf.edu/etd/5873

► Although Malaria rates are on the decline due to the efforts of the World Health Organization and other organizations dedicated to the eradication of this… (more)

▼ Although Malaria rates are on the decline due to the efforts of the World Health Organization and other organizations dedicated to the eradication of this disease, a relaxed attitude towards the development of new antimalarial entities would be flawed. Due to the emergence of resistance in the parasite, the almost 50% world-wide reduction in malarial death rates that have been produced over the past 15 years are threatening to be lost New drugs are urgently needed and our approach focuses on the re-evaluation and optimization of the historic antimalarial ICI 56,780. Due to its causal prophylactic activity, along with its ability to prevent transmission and potent blood schizonticidal activities, it was revisited with the hopes of first understanding which functionalities were responsible to the compound's activity. Secondly, we wanted to optimize the substituents in the 3, 6 and 7-positions. Finally and most importantly, we wanted to address the cross-resistance problem of the ICI 56,780 scaffold. Initial, analogues showed the importance of the ester in facilitating the convergence of the RI towards 1. Although those analogues lost activity in W2, TM90-C2B, and Pb, they were our first glimpse at this important trend that was later exploited in our 3-halo-6-butyl-7-(2-phenoxyethoxy)quinolin-4(1H)-one and 3-halo-6-butyl-2-methyl-7-(2-phenoxyethoxy)quinolin-4(1H)-ones which showed RI values of < 5 for our best analogues. Although our lead compound 3-bromo-6-butyl-2-methyl7-(2phenoxyethoxy)quinolin-4(1H) one possessed decreased activities as compared to ICI 56,780 at 2.60 nM for W2, 12.2 nM for TM90-C2B and 2.12 nM for Pb, it had 100% inhibition of parasite development on day 6 PE in our scouting assay and 61% inhibition on day 6in our Thompson model, increased from the < 2% value given by the ICI 56,780. Solubility and unfavorable in vivo stability were still major issues for this scaffold. Therefore, a series of piperazinyl 4(1H)-quinolones with greatly enhanced solubility were designed and tested in detailed structure activity relationships and structure property relationship studies. Initial results showed that 7-piperazinyl-4(1H)-quinolones possessed greatly increased solubilities when compared to ICI 56,780 analogues. Primarily, the linker length and the piperazine core was probed. This showed that compounds with a single carbon spacer were most active. Further testing of the 6-position gave methyl 6-methyl-4-oxo-7-((4-phenylpiperazin-1-yl)methyl)-1,4-dihydroquinoline-3-carboxylate with W2 and TM90-C2B values of 0.435 nM and 147 nM respectively. Substitution on the piperazinyl phenyl gave the most active compounds however the RI of >1500 was unacceptable. Because of this, 3-halo substituents were added to these quinolones with promising results. With RIs of < 3, the compounds were promising, however they were not active in vivo. However, methyl 6-methoxy-4-oxo-7-((4-(4-(trifluoromethyl)phenyl)piperazin-1-yl)methyl)-1,4-dihydroquinoline-3-carboxylate and methyl…

Subjects/Keywords: Malaria; Solubility; Piperazine; SAR; SPR; Chemistry; Parasitology

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APA (6^th Edition):

Maignan, J. R. (2015). Development of Orally Bioavailable 4(1H)-Quinolones and 1,2,3,4-Tetrahydroacridin-9(10H)-ones with Potent Anti-malarial Activity. (Thesis). University of South Florida. Retrieved from https://scholarcommons.usf.edu/etd/5873

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Maignan, Jordany Richarlson. “Development of Orally Bioavailable 4(1H)-Quinolones and 1,2,3,4-Tetrahydroacridin-9(10H)-ones with Potent Anti-malarial Activity.” 2015. Thesis, University of South Florida. Accessed January 18, 2021. https://scholarcommons.usf.edu/etd/5873.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Maignan, Jordany Richarlson. “Development of Orally Bioavailable 4(1H)-Quinolones and 1,2,3,4-Tetrahydroacridin-9(10H)-ones with Potent Anti-malarial Activity.” 2015. Web. 18 Jan 2021.

Vancouver:

Maignan JR. Development of Orally Bioavailable 4(1H)-Quinolones and 1,2,3,4-Tetrahydroacridin-9(10H)-ones with Potent Anti-malarial Activity. [Internet] [Thesis]. University of South Florida; 2015. [cited 2021 Jan 18]. Available from: https://scholarcommons.usf.edu/etd/5873.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Maignan JR. Development of Orally Bioavailable 4(1H)-Quinolones and 1,2,3,4-Tetrahydroacridin-9(10H)-ones with Potent Anti-malarial Activity. [Thesis]. University of South Florida; 2015. Available from: https://scholarcommons.usf.edu/etd/5873

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universitat de Valencia

16. González Bulnes, Luis. Interacción entre ácidos nucleicos y ligandos de bajo peso molecular: mecanismos moleculares de reconocimiento específico .

Degree: 2015, Universitat de Valencia

URL: http://hdl.handle.net/10550/45858

► Los ácidos nucleicos desempeñan una gran variedad de funciones bioquímicas y son moléculas básicas para la vida. El estudio de sus complejos con moléculas de… (more)

▼ Los ácidos nucleicos desempeñan una gran variedad de funciones bioquímicas y son moléculas básicas para la vida. El estudio de sus complejos con moléculas de bajo peso molecular es esencial para la búsqueda de nuevos agentes terapéuticos basados en la interacción con estas biomoléculas. Tras analizar la estructura de los ácidos nucleicos y los fundamentos de las técnicas experimentales utilizadas, esta tesis se centra en primer lugar en el estudio de la interacción entre ligandos bisnaftalimídicos antitumorales y diferentes secuencias de ADN, con el objetivo de identificar los mecanismos de reconocimiento específico del ADN por parte de estos compuestos. Utilizando una combinación de técnicas biofísicas, primeramente se evaluó el efecto de modificar las secuencias que flanquean el sitio de unión preferido de la bisnaftalimida de referencia elinafide. Se observaron diferencias significativas, especialmente en el caso de complejos flanqueados por tractos A, que manifestaron una afinidad más baja, interacciones de apilamiento más débiles, y procesos dinámicos más rápidos. Por lo tanto, el reconocimiento del ADN por parte de elinafide está fuertemente modulado por efectos indirectos, dependientes de las preferencias conformacionales de cada secuencia. A continuación se estudió el mecanismo de reconocimiento directo del ADN por parte de ligandos bisnaftalimídicos. Los resultados indicaron que este proceso depende fundamentalmente de los contactos electrostáticos establecidos entre el espaciador aminoalquílico de las bisnaftalimidas y los grupos electronegativos de guanina en el surco mayor, que determinan tanto la especificidad de los ligandos por pasos GpC como el proceso de intercalación de los anillos de naftalimida. Además, se concluyó que la interacción entre el ADN y las bisnaftalimidas tiene lugar a través de dos estados: unión al surco e intercalación. En consonancia con este modelo de dos estados, cambios en distintos factores como el pH, la fuerza iónica o la composición de las bases del ADN o los anillos aromáticos de los compuestos tuvieron efectos diferentes en los parámetros de unión determinados por métodos biofísicos habitualmente utilizados en el estudio de complejos formados por ácidos nucleicos. El segundo capítulo de resultados de esta tesis describe el diseño de novo de moléculas p-terfenílicas bilateralmente substituídas y el estudio de su interaccióncon el subdominio IIB del Elemento de Reconocimiento de Rev (RRE) del ARN genómico del VIH-1, con el propósito de bloquear la interacción entre RRE y la alfa -hélice de unión a ARN de Rev y ejercer un efecto inhibitorio sobre la replicación del virus. Para ello se utilizó una combinación de métodos biofísicos y de técnicas computacionales basadas en estructura. Los resultados demostraron que los sustituyentes bilaterales de estas moléculas se proyectan en un ángulo de 360o, y que estos ligandos actúan como miméticos de Rev en su complejo con RRE, siendo capaces de desplazar la alfa -hélice del bucle IIB de RRE. Por… Advisors/Committee Members: Gallego Sala, José (advisor).

Subjects/Keywords: ADN; ARN; fármaco; interacción; ligando; RMN; SPR

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APA (6^th Edition):

González Bulnes, L. (2015). Interacción entre ácidos nucleicos y ligandos de bajo peso molecular: mecanismos moleculares de reconocimiento específico . (Doctoral Dissertation). Universitat de Valencia. Retrieved from http://hdl.handle.net/10550/45858

Chicago Manual of Style (16^th Edition):

González Bulnes, Luis. “Interacción entre ácidos nucleicos y ligandos de bajo peso molecular: mecanismos moleculares de reconocimiento específico .” 2015. Doctoral Dissertation, Universitat de Valencia. Accessed January 18, 2021. http://hdl.handle.net/10550/45858.

MLA Handbook (7^th Edition):

González Bulnes, Luis. “Interacción entre ácidos nucleicos y ligandos de bajo peso molecular: mecanismos moleculares de reconocimiento específico .” 2015. Web. 18 Jan 2021.

Vancouver:

González Bulnes L. Interacción entre ácidos nucleicos y ligandos de bajo peso molecular: mecanismos moleculares de reconocimiento específico . [Internet] [Doctoral dissertation]. Universitat de Valencia; 2015. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/10550/45858.

Council of Science Editors:

González Bulnes L. Interacción entre ácidos nucleicos y ligandos de bajo peso molecular: mecanismos moleculares de reconocimiento específico . [Doctoral Dissertation]. Universitat de Valencia; 2015. Available from: http://hdl.handle.net/10550/45858

University of Cambridge

17. Du, Yao. Particle-modified surface plasmon resonance biosensor.

Degree: PhD, 2019, University of Cambridge

URL: https://doi.org/10.17863/CAM.36636 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.767920

► Surface plasmon resonance (SPR) biosensors have attracted great attention in scientific research in the past three decades. Extensive studies on the immobilisation of biorecognition elements… (more)

▼ Surface plasmon resonance (SPR) biosensors have attracted great attention in scientific research in the past three decades. Extensive studies on the immobilisation of biorecognition elements have been conducted in pursuit of higher sensitivity, but trialled formats have focussed on a thin layer modification next to the plasmon film, which usually requires in situ derivatization. This thesis investigates an 'off-chip' immobilisation strategy for SPR biosensing using silica particles and considers the implications of a particle-modified evanescent field on the signal amplitude and kinetics, for an exemplar affinity binding between immobilised IgG and its anti-IgG complement. Submicron silica particles were synthesized as carriers for the bio-recognition elements. They were then immobilised to form a sub-monolayer on the gold film of an SPR biosensor using two methods: thiolsilane coupling and physical adsorption aided by mechanical pressure. The bio-sensitivity towards an antigen/antibody interaction was lower than an SPR biosensor with an alkanethiolate SAM due to the difference in ligand capacity and position in the evanescent field. The binding kinetics of antigen/antibody pair was found to follow the Langmuir model closely in a continuous flow configuration but was heavily limited by the mass transport from the bulk to the sensor surface in a stop-flow configuration. A packed channel configuration was designed with larger gel particles as ligand carriers, packed on top of a gold film to create a column-modified SPR biosensor. This sensor has comparable bio-sensitivity to the previous sub-monolayer particle-modified systems, but the binding and dissociation of the analyte was heavily dependent on mass transport and binding equilibria across the column. A bi-directional diffusion mechanism was proposed based on a two-compartment mass transport model and the expanded model fitted well with the experimental data. The column-modified sensor was also studied by SPR imaging and analyte band formation was observed and analysed. Using the lateral resolution, a multiplexing particle column configuration was explored, and its potential in distinguishing a multicomponent analyte.

Subjects/Keywords: SPR; surface plasmon resonance; biosensor; silica particles

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Du, Y. (2019). Particle-modified surface plasmon resonance biosensor. (Doctoral Dissertation). University of Cambridge. Retrieved from https://doi.org/10.17863/CAM.36636 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.767920

Chicago Manual of Style (16^th Edition):

Du, Yao. “Particle-modified surface plasmon resonance biosensor.” 2019. Doctoral Dissertation, University of Cambridge. Accessed January 18, 2021. https://doi.org/10.17863/CAM.36636 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.767920.

MLA Handbook (7^th Edition):

Du, Yao. “Particle-modified surface plasmon resonance biosensor.” 2019. Web. 18 Jan 2021.

Vancouver:

Du Y. Particle-modified surface plasmon resonance biosensor. [Internet] [Doctoral dissertation]. University of Cambridge; 2019. [cited 2021 Jan 18]. Available from: https://doi.org/10.17863/CAM.36636 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.767920.

Council of Science Editors:

Du Y. Particle-modified surface plasmon resonance biosensor. [Doctoral Dissertation]. University of Cambridge; 2019. Available from: https://doi.org/10.17863/CAM.36636 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.767920

Johannes Gutenberg Universität Mainz

18. Plum, Markus Alexander. Messung dynamischer Prozesse in der Nähe von Grenzflächen mit Hilfe neu entwickelter Lichtstreumethoden.

Degree: 2010, Johannes Gutenberg Universität Mainz

URL: http://ubm.opus.hbz-nrw.de/volltexte/2011/2615/

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Die vorliegende Arbeit behandelt die Entwicklung und Erprobung zweier neuer dynamischer Lichtstreumethoden: Die Resonanz verstärkte Lichtstreuung (REDLS: resonance enhanced dynamic light scattering) und die Wellenleiter… (more)

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Die vorliegende Arbeit behandelt die Entwicklung und Erprobung zweier neuer dynamischer Lichtstreumethoden: Die Resonanz verstärkte Lichtstreuung (REDLS: resonance enhanced dynamic light scattering) und die Wellenleiter verstärkte Lichtstreuung (WEDLS: waveguide enhanced dynamic light scattering). Beide Methoden verwenden eine Kombination aus evaneszenten Wellen und dynamischer Lichtstreuung: Bei der REDLS-Technik wird das evaneszentes Feld eines Oberflächenplasmons verwendet, bei der WEDLS-Technik handelt es sich um das evaneszente Feld von Metallfilm verstärkten Leckwellenleitermoden. Die neuen Methoden liefern Informationen über die Dynamik an Grenzflächen über ein breites Zeitfenster (einige Nanosekunden bis hin zu mehreren Sekunden) mit einer räumlichen Auflösung im sub-Mikrometerbereich. Sie erweitern somit das Gebiet der dynamischen Lichtstreuung in evaneszenter Geometrie, bei dem bislang nur die evanescent wave dynamic light scattering (EWDLS) - Technik zur Verfügung stand. Bei der EWDLS-Technik wird das evaneszente Feld der Totalreflexion als kohärenter Lichtstrahl für die dynamische Lichtstreuung verwendet. Ein Vergleich mit der EWDLS-Technik zeigt ein stark erhöhtes Signal/Rausch-Verhältnis bei den neu entwickelten Techniken aufgrund der resonanten Anregung. Zusätzlich ist es sowohl bei der REDLS- als auch bei der WEDLS-Technik möglich Grenzflächenmodifikationen und damit z.B. Adsorptionsprozesse zu detektieren. Der Einfluss einer Grenzfläche auf die Diffusion von PS-Latex-Partikeln wurde untersucht. Die Grenzfläche bestand im Fall der REDLS-Technik aus Gold, bei der WEDLS-Technik aus PMMA. Die Funktionsweise und die Gültigkeit der neu entwickelten Techniken wurde mit Hilfe von PS-Latex-Partikeln mit hydrodynamischen Radien von R =11nm bis hin zu R=204nm demonstriert.

To monitor physical properties in the near-interface region with spatial and temporal resolution demands for powerful experimental techniques. In the last years evanescent waves generated by total internal reflection have attracted a great deal of interest as source of light for dynamic light scattering, because of their strong localization to the interface. The main disadvantages are the generally weak signal and the missing feature of surface monitoring. This thesis is about the development of two new experimental methods of dynamic light scattering close to an interface with evanescent waves as sources of light in order to boost the signal and to monitor this interface at the same time: In Resonance Enhanced Dynamic Light Scattering (REDLS) surface plasmons are utilized as an incident electromagnetic field in order to measure relaxation functions with laser power of less than a milliwatt at a predetermined penetration depth. In waveguide enhanced dynamic light scattering, (WEDLS) the evanescent part of waveguide modes offers an even stronger increase in signal, allows surface monitoring and in situ variation of the penetration depth. The effect of an interface on the diffusion of polystyrene latex particles in dilute solution was…

Subjects/Keywords: REDLS; WEDLS; SPR; DLS; REDLS; WEDLS; SPR; DLS; Natural sciences and mathematics

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Plum, M. A. (2010). Messung dynamischer Prozesse in der Nähe von Grenzflächen mit Hilfe neu entwickelter Lichtstreumethoden. (Doctoral Dissertation). Johannes Gutenberg Universität Mainz. Retrieved from http://ubm.opus.hbz-nrw.de/volltexte/2011/2615/

Chicago Manual of Style (16^th Edition):

Plum, Markus Alexander. “Messung dynamischer Prozesse in der Nähe von Grenzflächen mit Hilfe neu entwickelter Lichtstreumethoden.” 2010. Doctoral Dissertation, Johannes Gutenberg Universität Mainz. Accessed January 18, 2021. http://ubm.opus.hbz-nrw.de/volltexte/2011/2615/.

MLA Handbook (7^th Edition):

Plum, Markus Alexander. “Messung dynamischer Prozesse in der Nähe von Grenzflächen mit Hilfe neu entwickelter Lichtstreumethoden.” 2010. Web. 18 Jan 2021.

Vancouver:

Plum MA. Messung dynamischer Prozesse in der Nähe von Grenzflächen mit Hilfe neu entwickelter Lichtstreumethoden. [Internet] [Doctoral dissertation]. Johannes Gutenberg Universität Mainz; 2010. [cited 2021 Jan 18]. Available from: http://ubm.opus.hbz-nrw.de/volltexte/2011/2615/.

Council of Science Editors:

Plum MA. Messung dynamischer Prozesse in der Nähe von Grenzflächen mit Hilfe neu entwickelter Lichtstreumethoden. [Doctoral Dissertation]. Johannes Gutenberg Universität Mainz; 2010. Available from: http://ubm.opus.hbz-nrw.de/volltexte/2011/2615/

19. Carregaro, Fernanda. Estudo das proteínas pequenas ricas em prolina (SPRRs) em câncer de cabeça e pescoço.

Degree: PhD, Biologia (Genética), 2012, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/41/41131/tde-18012013-153012/ ;

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As proteínas pequenas ricas em prolina (small proline-rich protein/SPRRs) compreendem uma sub-classe específica de precursores da camada córnea, codificadas por uma família multi-gênica do complexo… (more)

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As proteínas pequenas ricas em prolina (small proline-rich protein/SPRRs) compreendem uma sub-classe específica de precursores da camada córnea, codificadas por uma família multi-gênica do complexo de diferenciação epidérmica mapeado no cromossomo 1q21. Vários estudos têm sugerido que as SPRs estão relacionadas com proliferação epitelial elevada e processos malignos. O presente trabalho teve como objetivo investigar a participação das SPRs no desenvolvimento de carcinomas epidermóides de cabeça e do pescoço e no fenótipo da célula neoplásica. O perfil de expressão de onze genes SPRRs foi avaliado em cinco linhagens celulares (FADU, HEP-2, UM-SCC-38, SCC-9) e em carcinomas primários da cabeça e pescoço, utilizando PCR em tempo real. Os tumores foram classificados em agressivos (A) e menos agressivos (LA) dependendo da presença ou da ausência de células neoplásicas nos nódulos linfáticos regionais. Os resultados revelaram baixa expressão de genes SPRRs em todas as linhagens celulares, exceto o SPRR4. Por outro lado, foram observados níveis elevados de transcritos de SPRR2G, SPRR4 e SPRR2E e níveis reduzidos de transcritos de SPRR3 tanto nos grupos A e LA de carcinomas. A expressão ectópica de SPRR2E resultou em menor capacidade de invasão celular em ensaio de câmara de Boyden, bem como em mudança do fenótipo epitelial para fibroblastóide, mas não afetou a proliferação celular ou a migração. Após o tratamento das células HEP-2 com a proteína anti-inflamatória anexina A1 (peptídeo AC2-26), ocorreu um aumento substancial de expressão da maioria dos SPRRs, sugerindo uma associação entre SPRs e inflamação. Os genes SPRRs possuem sequências altamente conservadas e, após uma análise filogenética utilizando o método de neighbor-joining, os resultados indicaram um grupo homogêneo, que incluiu SPRR2 e SPRR4, e um grupo mais heterogêneo com SPRR1 e SPRR3. Aparentemente, a diversidade de sequência destes genes é baixa. Isto sugere que o controle da dosagem da proteína pode ser mais importante para o desempenho de sua função que a complexidade estrutural. A compreensão da função das SPRs avançou bastante nos últimos anos, mas muitas questões sobre o seu papel no processo neoplásico ainda permanecem sem resposta. Muitos dados são ainda necessários para identificar sua associação com outras proteínas e com vias de sinalização. Portanto, é importante melhorar nosso conhecimento sobre a regulação e a função de cada RPN, para que as descobertas obtidas na pesquisa básica sejam traduzidas em aplicações clínicas

The small proline rich proteins (SPRs) constitute a specific sub-class of cornified cell envelope precursors, encoded by a multi-gene family which are part of the epidermal differentiation complex on chromosome 1q21. Several studies have suggested that the SPRs are related to increased epithelial proliferation and to malignant processes. The present work aimed to investigate the participation of SPRs in the development of head and neck squamous cell carcinomas and in the phenotype of the neoplastic cell. The expression profile…

Advisors/Committee Members: Silva, Eloiza Helena Tajara da.

Subjects/Keywords: 2.SPR; Câncer de cabeça e pescoço; Head and meck cancer; SPR; SPRR; SPRR

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Carregaro, F. (2012). Estudo das proteínas pequenas ricas em prolina (SPRRs) em câncer de cabeça e pescoço. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/41/41131/tde-18012013-153012/ ;

Chicago Manual of Style (16^th Edition):

Carregaro, Fernanda. “Estudo das proteínas pequenas ricas em prolina (SPRRs) em câncer de cabeça e pescoço.” 2012. Doctoral Dissertation, University of São Paulo. Accessed January 18, 2021. http://www.teses.usp.br/teses/disponiveis/41/41131/tde-18012013-153012/ ;.

MLA Handbook (7^th Edition):

Carregaro, Fernanda. “Estudo das proteínas pequenas ricas em prolina (SPRRs) em câncer de cabeça e pescoço.” 2012. Web. 18 Jan 2021.

Vancouver:

Carregaro F. Estudo das proteínas pequenas ricas em prolina (SPRRs) em câncer de cabeça e pescoço. [Internet] [Doctoral dissertation]. University of São Paulo; 2012. [cited 2021 Jan 18]. Available from: http://www.teses.usp.br/teses/disponiveis/41/41131/tde-18012013-153012/ ;.

Council of Science Editors:

Carregaro F. Estudo das proteínas pequenas ricas em prolina (SPRRs) em câncer de cabeça e pescoço. [Doctoral Dissertation]. University of São Paulo; 2012. Available from: http://www.teses.usp.br/teses/disponiveis/41/41131/tde-18012013-153012/ ;

20. Rouhana, Jad. Etude et modulation des interactions protéine-protéine : l’activation de la petite protéine G Arf1 par son facteur d’échange Arno : Study and modulation of protein-protein interactions : Activation of the small G protein (Arf1) by its guanidine exchange factor (ARNO).

Degree: Docteur es, Biologie Santé, 2013, Université Montpellier I

URL: http://www.theses.fr/2013MON13507

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Arf1 est une petite protéine G (pG), essentiellement impliquée dans le trafic vésiculaire. Arf1 oscille entre deux conformations, l'une active liée au GTP et l'autre… (more)

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Arf1 est une petite protéine G (pG), essentiellement impliquée dans le trafic vésiculaire. Arf1 oscille entre deux conformations, l'une active liée au GTP et l'autre inactive associée au GDP. Arno est un des facteurs d'échange (GEF) capable d'activer Arf1 en stimulant l'échange GDP/GTP. Suractivée dans les cellules invasives du cancer du sein, Arf1 joue un rôle important dans la migration et la prolifération des cellules cancéreuses.Le but de ma thèse s'inscrit dans l'étude et la modulation de l'interaction pG-GEF, et plus spécifiquement, le couple Arf1-Arno. Mon travail a été planifié autour de deux axes: (1) L'étude fine de l'interaction entre Arf1 et Arno, et sa modulation avec un inhibiteur connu la Bréféldine A (BFA). (2) La mise en place d'une stratégie de conception d'inhibiteurs de l'interaction protéine-protéine du couple Arf1-Arno.Dans un premier temps, nous avons mis en place une méthode basée sur la résonance plasmonique de surface (SPR) permettant la détermination des paramètres cinétiques de l'interaction entre Arf1 et Arno. Nous avons précisé aussi les conséquences des partenaires allostériques (GDP, GTP, et Mg2+) et de la BFA sur les paramètres cinétiques de l'interaction. Ceci a permis une analyse fine de la régulation allostérique et du mode d'action de la BFA. Appliquée à d'autres inhibiteurs, cette méthode permettra d'examiner leur mécanisme d'inhibition.Dans la deuxième partie j'expose, la stratégie que nous avons utilisé pour la conception rationnelle d'inhibiteur de l'interaction entre Arf1 et Arno. Elle est basée sur le criblage virtuel de fragments au niveau des résidus clé « hotspots » de l'interaction, la validation des molécules-touches par des techniques biophysiques, et l'élimination de molécules artefacts. Les structures des complexes fragments-Arno ont été résolues, ce qui confirme la validité de cette stratégie ouvrant la voie vers l'optimisation moléculaire pour obtenir des inhibiteurs plus efficaces.

Arf1 is a small GTPases, essentially involved in the vesicular traffic. Arf1 switch between two conformations, an active form bound to GTP and an inactive form bound to GDP. Arno is one of the exchange factors (GEF) that can activate Arf1, through its catalytic Sec7 domain, promoting the exchange of GDP by GTP. Activated in breast cancer cells, Arf1 plays an important role in the migration and proliferation of cancer cells.The aim of my thesis was the study and the modulation of the interaction between small G proteins and their GEFs, more precisely the Arf1-Arno interaction. My work has been planned around two axes: (1) the study of the interaction between Arf1 and Arno, and its modulation with a known inhibitor Brefeldin A (BFA). (2) The development of a rational strategy for designing inhibitors of protein-protein interaction for the Arf1-Arno complex.In the first part of my PhD work, we set up a Surface Plasmon Resonance (SPR) method allowing to determine the kinetic parameters of the interaction between Arf1 and Arno. We also studied the effects of allosteric partners such as…

Advisors/Committee Members: Chavanieu, Alain (thesis director), Padilla, André (thesis director).

Subjects/Keywords: Fragment-based drug design; Spr; Interaction protéine-protéine; Fbdd; Spr; Protein-Protein Interaction; 577

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Rouhana, J. (2013). Etude et modulation des interactions protéine-protéine : l’activation de la petite protéine G Arf1 par son facteur d’échange Arno : Study and modulation of protein-protein interactions : Activation of the small G protein (Arf1) by its guanidine exchange factor (ARNO). (Doctoral Dissertation). Université Montpellier I. Retrieved from http://www.theses.fr/2013MON13507

Chicago Manual of Style (16^th Edition):

Rouhana, Jad. “Etude et modulation des interactions protéine-protéine : l’activation de la petite protéine G Arf1 par son facteur d’échange Arno : Study and modulation of protein-protein interactions : Activation of the small G protein (Arf1) by its guanidine exchange factor (ARNO).” 2013. Doctoral Dissertation, Université Montpellier I. Accessed January 18, 2021. http://www.theses.fr/2013MON13507.

MLA Handbook (7^th Edition):

Rouhana, Jad. “Etude et modulation des interactions protéine-protéine : l’activation de la petite protéine G Arf1 par son facteur d’échange Arno : Study and modulation of protein-protein interactions : Activation of the small G protein (Arf1) by its guanidine exchange factor (ARNO).” 2013. Web. 18 Jan 2021.

Vancouver:

Rouhana J. Etude et modulation des interactions protéine-protéine : l’activation de la petite protéine G Arf1 par son facteur d’échange Arno : Study and modulation of protein-protein interactions : Activation of the small G protein (Arf1) by its guanidine exchange factor (ARNO). [Internet] [Doctoral dissertation]. Université Montpellier I; 2013. [cited 2021 Jan 18]. Available from: http://www.theses.fr/2013MON13507.

Council of Science Editors:

Rouhana J. Etude et modulation des interactions protéine-protéine : l’activation de la petite protéine G Arf1 par son facteur d’échange Arno : Study and modulation of protein-protein interactions : Activation of the small G protein (Arf1) by its guanidine exchange factor (ARNO). [Doctoral Dissertation]. Université Montpellier I; 2013. Available from: http://www.theses.fr/2013MON13507

21. Lisi, Samuele. Approches innovantes basées sur la Résonance des Plasmons de Surface pour le diagnostic biomoléculaire de la maladie d’Alzheimer : Novel approaches based on Surface Plasmon Resonance biosensor formolecular diagnosis of Alzheimer's disease.

Degree: Docteur es, Chimie Biologie, 2017, Université Grenoble Alpes (ComUE); Università degli studi (Florence, Italie)

URL: http://www.theses.fr/2017GREAV003

► La maladie d’Alzheimer est une pathologie neurodégénérative qui amène à une perte progressive de la mémoire et cause des changements comportementaux. Selon plusieurs théories, le… (more)

▼ La maladie d’Alzheimer est une pathologie neurodégénérative qui amène à une perte progressive de la mémoire et cause des changements comportementaux. Selon plusieurs théories, le développement de cette maladie est associé à l’accumulation du peptide amyloïde beta et de la protéine tau dans des zones précises du cerveau humain. A l’heure actuelle, les approches thérapeutiques testées sont fondées sur l’hypothèse de la cascade amyloïde, mais les résultats n’ont pas été jugés suffisamment efficaces. Pour augmenter les chances de succès des traitements thérapeutiques existants, de meilleures techniques pour un dépistage précoce de l’Alzheimer semblent nécessaires. De ce fait, dans cette thèse, des stratégies innovantes pour l’analyse d’un des biomarqueurs de la maladie d’Alzheimer sont proposées. En particulier le projet porte sur l’analyse de la protéine tau avec des biocapteurs basés sur la Résonance de Plasmons de Surface (SPR). L’augmentation du niveau de ce biomarqueur dans le Liquide Céphalo-Rachidien (LCR) est déjà indicateur d’un processus de neurodégénérescence. De plus, si la mesure de la protéine tau est combinée à celle d’autres biomarqueurs de la pathologie (i.e. : amyloïde beta), les possibilités de dépistage sont fortement augmentées. Les travaux ont portés sur deux aspects : initialement l’interaction antigène-anticorps a été exploitée pour développer un immunocapteur pour la protéine tau. En utilisant cette technologie, nous avons pu caractériser les paramètres analytiques de l’essai direct (avec un seul anticorps) et ceux de l’essai sandwich (avec deux anticorps complémentaires). Dès ces premières approches, nous avons remarqué le besoin d’augmenter la sensibilité de la méthode SPR développée. En effet la limite de détection pour l’essai sandwich était de l’ordre du nM, alors que les niveaux de tau dans le LCR sont de l’ordre du pM. L’utilisation de nanotechnologies, en particulier des nanotubes de carbone, a permis d’atteindre des niveaux proches du pM, avec de bonnes performances en terme de répétabilité de l’essai.Une approche alternative a été conçue dans la deuxième partie du projet. Elle était consacrée à la sélection d’un aptamère pour la protéine tau, afin d’exploiter les avantages de cette classe de récepteurs par rapport aux anticorps. Pour accomplir cet objectif, deux stratégies de sélection ont été mises en place. Premièrement la sélection traditionnelle (SELEX, Systematic Evolution of Ligands by EXponential enrichment) a été appliquée en utilisant l’Electrophorèse Capillaire (EC) comme moyen de séparation. Bien que de nombreuses conditions aient été modifiées, avec le SELEX traditionnel nous n’avons pas observé une évolution significative de l’affinité entre les séquences d’ADN et la protéine tau. Dans la deuxième approche nous avons utilisé la même méthode de séparation pour mener la sélection à travers l’EC-Non-SELEX. En utilisant cette méthode, où les étapes de PCR étaient réduites, une évolution positive a été observée après seulement trois rounds. En effet cinq séquences parmi celles… Advisors/Committee Members: Peyrin, Eric (thesis director), Minunni, Maria (thesis director).

Subjects/Keywords: Biocapteurs; Aptamères; Electrophorese capillaire-SELEX; Spr; Biosensing; Aptamers; Capillary electrophoresis; Spr; 610

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lisi, S. (2017). Approches innovantes basées sur la Résonance des Plasmons de Surface pour le diagnostic biomoléculaire de la maladie d’Alzheimer : Novel approaches based on Surface Plasmon Resonance biosensor formolecular diagnosis of Alzheimer's disease. (Doctoral Dissertation). Université Grenoble Alpes (ComUE); Università degli studi (Florence, Italie). Retrieved from http://www.theses.fr/2017GREAV003

Chicago Manual of Style (16^th Edition):

Lisi, Samuele. “Approches innovantes basées sur la Résonance des Plasmons de Surface pour le diagnostic biomoléculaire de la maladie d’Alzheimer : Novel approaches based on Surface Plasmon Resonance biosensor formolecular diagnosis of Alzheimer's disease.” 2017. Doctoral Dissertation, Université Grenoble Alpes (ComUE); Università degli studi (Florence, Italie). Accessed January 18, 2021. http://www.theses.fr/2017GREAV003.

MLA Handbook (7^th Edition):

Lisi, Samuele. “Approches innovantes basées sur la Résonance des Plasmons de Surface pour le diagnostic biomoléculaire de la maladie d’Alzheimer : Novel approaches based on Surface Plasmon Resonance biosensor formolecular diagnosis of Alzheimer's disease.” 2017. Web. 18 Jan 2021.

Vancouver:

Lisi S. Approches innovantes basées sur la Résonance des Plasmons de Surface pour le diagnostic biomoléculaire de la maladie d’Alzheimer : Novel approaches based on Surface Plasmon Resonance biosensor formolecular diagnosis of Alzheimer's disease. [Internet] [Doctoral dissertation]. Université Grenoble Alpes (ComUE); Università degli studi (Florence, Italie); 2017. [cited 2021 Jan 18]. Available from: http://www.theses.fr/2017GREAV003.

Council of Science Editors:

Lisi S. Approches innovantes basées sur la Résonance des Plasmons de Surface pour le diagnostic biomoléculaire de la maladie d’Alzheimer : Novel approaches based on Surface Plasmon Resonance biosensor formolecular diagnosis of Alzheimer's disease. [Doctoral Dissertation]. Université Grenoble Alpes (ComUE); Università degli studi (Florence, Italie); 2017. Available from: http://www.theses.fr/2017GREAV003

Université de Grenoble

22. Sevajol, Marion. Caractérisation structurale et biophysique de Elmo1 et des interactions avec son partenaire : Structural and biophysical characterisation of Elmo1 and of interactions with a binding partner : A protein involved in actin cytoskeleton remodeling pathway.

Degree: Docteur es, Physique, 2012, Université de Grenoble

URL: http://www.theses.fr/2012GRENY104

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Les protéines eucaryotes Elmo (Engulfment and Cell Motility) forment une famille de régulateurs conservés qui jouent un rôle central dans les processus biologiques reposant sur… (more)

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Les protéines eucaryotes Elmo (Engulfment and Cell Motility) forment une famille de régulateurs conservés qui jouent un rôle central dans les processus biologiques reposant sur le remodelage du cytosquelette d'actine comme la phagocytose et la migration cellulaire et régulé par les GTPases de la famille Rho. Les protéines Elmo régulent la fonction des protéines Dock (Downstream of Crk), qui sont des Facteurs d'Echange de Guanine (GEF) atypiques pour les GTPases Rac1 et Cdc42. Le mécanisme de régulation connu à ce jour, repose sur l'interaction entre les 200 résidus C-terminaux d'Elmo et les 180 premiers résidus N-terminaux de Dock. Cependant, le rôle précis des différents domaines et motifs identifiés dans ces régions n'est pas encore défini. En effet, les données fonctionnelles, biochimiques et structurales rapportées à ce jour semblent contradictoires quant à la contribution de l'extrémité C-terminale d'Elmo qui comprend un motif polyproline et le domaine SH3 N-terminal de Dock. Nous avons donc étudié la contribution de l'extrémité C-terminale de Elmo1 à l'interaction entre Elmo1 et le domaine SH3 de Dock1 en utilisant notamment la résonance plasmonique de surface. Nos données démontrent la capacité du domaine SH3 de Dock1 à interagir avec Elmo1, indépendamment de l'extrémité C-terminale contenant le motif polyproline. Toutefois, la présence de cette région conduit à une augmentation significative du temps de demi-vie du complexe Elmo1/Dock1. En parallèle, des expériences de diffusion des rayons X aux petits angles nous ont permis de déterminer des enveloppes tridimensionnelles de la protéine Elmo1. Ces données nous permettent ainsi de proposer un premier modèle à basse résolution dans lequel nous localisons les parties N et C-terminales de Elmo1. De façon surprenante cette étude semble indiquer un changement de conformation de la région N-terminale de Elmo1 ainsi qu'une interaction possible de cette même région avec le domaine SH3 de Dock1.

The eukaryotic Elmo proteins (EnguLfment and cell MOtility) form a conserved regulatory family that plays a central role in a number of processes that depend on actin cytoskeleton remodeling, such as phagocytosis and cell migration. Elmo proteins regulate the function of Dock proteins (Downstream of CrK), a new family of atypical guanine exchange factors (GEF) for Rac1 and Cdc42 GTPases. The regulation of this mechanism is based on the interaction between the 200 C-terminal residues of Elmo and the 180 N-terminal residues of Dock. However, the precise role of the different domains and motifs identified in these regions is still not well defined. Indeed, functional, structural and biochemical data reported to date seem contradictory with respect to the contribution of the C-terminal end of Elmo, which includes a polyproline motif, and the N-terminal SH3 domain of Dock. We have therefore investigated the contribution of the C-terminal region of Elmo1 to the interaction between Elmo1 and the SH3 domain of Dock1 using surface plasmon resonance. Our data demonstrate the ability…

Advisors/Committee Members: Housset, Dominique (thesis director).

Subjects/Keywords: Elmo; Dock; Phagocytose; Migration cellulaire; SPR; SAXS; Elmo; Dock; Phagocytosis; Cell migration; SPR; SAXS

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sevajol, M. (2012). Caractérisation structurale et biophysique de Elmo1 et des interactions avec son partenaire : Structural and biophysical characterisation of Elmo1 and of interactions with a binding partner : A protein involved in actin cytoskeleton remodeling pathway. (Doctoral Dissertation). Université de Grenoble. Retrieved from http://www.theses.fr/2012GRENY104

Chicago Manual of Style (16^th Edition):

Sevajol, Marion. “Caractérisation structurale et biophysique de Elmo1 et des interactions avec son partenaire : Structural and biophysical characterisation of Elmo1 and of interactions with a binding partner : A protein involved in actin cytoskeleton remodeling pathway.” 2012. Doctoral Dissertation, Université de Grenoble. Accessed January 18, 2021. http://www.theses.fr/2012GRENY104.

MLA Handbook (7^th Edition):

Sevajol, Marion. “Caractérisation structurale et biophysique de Elmo1 et des interactions avec son partenaire : Structural and biophysical characterisation of Elmo1 and of interactions with a binding partner : A protein involved in actin cytoskeleton remodeling pathway.” 2012. Web. 18 Jan 2021.

Vancouver:

Sevajol M. Caractérisation structurale et biophysique de Elmo1 et des interactions avec son partenaire : Structural and biophysical characterisation of Elmo1 and of interactions with a binding partner : A protein involved in actin cytoskeleton remodeling pathway. [Internet] [Doctoral dissertation]. Université de Grenoble; 2012. [cited 2021 Jan 18]. Available from: http://www.theses.fr/2012GRENY104.

Council of Science Editors:

Sevajol M. Caractérisation structurale et biophysique de Elmo1 et des interactions avec son partenaire : Structural and biophysical characterisation of Elmo1 and of interactions with a binding partner : A protein involved in actin cytoskeleton remodeling pathway. [Doctoral Dissertation]. Université de Grenoble; 2012. Available from: http://www.theses.fr/2012GRENY104

23. Vindas Yassine, Karim. Résonance plasmon et développements instrumentaux vers la conception de biopuces et biocapteurs innovants : Development and optimization of new plasmon resonance based biochips and biosensors.

Degree: Docteur es, Physique pour les sciences du vivant, 2017, Université Grenoble Alpes (ComUE)

URL: http://www.theses.fr/2017GREAY091

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Ce travail de thèse porte sur la conception d’un « laboratoire-sur-fibre » original dédié à l’analyse moléculaire à distance, sans marquage et in vivo compatible… (more)

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Ce travail de thèse porte sur la conception d’un « laboratoire-sur-fibre » original dédié à l’analyse moléculaire à distance, sans marquage et in vivo compatible dans l’avenir avec les examens endoscopiques et dédié à l’assistance aux diagnostiques. Notre approche est basée sur l’utilisation de faisceaux de fibres microstructurés. Lorsqu’ils sont correctement conçus et recouverts d’une couche d’or, ces assemblages de fibres présentent des propriétés plasmoniques intéressantes. Dans un premier temps, le modèle numérique utilisé pour atteindre une meilleure compréhension des phénomènes physiques impliqués dans l’optimisation de la sensibilité des capteurs est expliqué. Les simulations, basées sur l’optique géométrique, ont été utilisées pour optimiser la géométrie des pointes et l’épaisseur de la couche d’or dans le but d’améliorer les performances analytiques et permettre ainsi des détections d’interactions biochimiques. Le processus de fabrication des capteurs est ensuite expliqué depuis leur structuration par gravure chimique effectuée à l’ISM (Bordeaux) jusqu’à leur métallisation réalisée au CEA Grenoble. Une comparaison entre les comportements théoriques et expérimentaux et alors menée pour comprendre l’influence de l’hétérogénéité du dépôt d’or et des surfaces gravées sur la sensibilité optique. Ces propriétés optiques sont ensuite exploitées jusqu’à la preuve de concept d’analyses biochimiques déportées. Cette étape a été réalisée en deux temps : d’abord la sensibilité à l’indice local a été démontrée en détectant l’adsorption d’une couche organique auto-assemblée et ensuite un suivi de l’interaction spécifique entre deux brins d’ADN complémentaires a été effectué. Le manuscrit s’achève par une analyse des aspects plus complexes liés à la nature peu multimodale des fibres présentes dans le faisceau. La théorie des guides d’ondes est alors utilisée pour expliquer l’influence du caractère modal de la propagation de la lumière sur les réponses des fibres optiques.

This Ph.D. thesis focuses on the design of an original “lab-on-fiber” tool for remote, label-free in vivo molecular analysis that could be dedicated in the future to endoscopic diagnosis. Our approach is based on functionalized microstructured optical fiber bundles. When appropriately designed and covered by a gold layer, those fibers exhibit interesting plasmonic properties. First, the numerical model used to reach a better understanding of the physical phenomena involved in the optimization of the sensor’s sensitivity is explained. The simulations based on ray optics were then used to optimize the fiber tip geometry and gold coating thickness to enhance the analytical performances and ultimately allow biochemical detections. The fabrication process of the sensor is then explained going from the chemical etching done by the ISM team (Bordeaux) to the metallization of the tips performed at the CEA Grenoble. A comparison between theoretical and experimental behaviors is then conducted to assess the influence of the heterogeneity of both the gold deposit…

Advisors/Committee Members: Buhot, Arnaud (thesis director), Leroy, Loïc (thesis director), Engel, Elodie (thesis director).

Subjects/Keywords: Biocapteurs; Plasmons; SPR; Fibres Optiques; Multiplexage; Biopuces; Biosensors; Plasmons; SPR; Optical fibers; Multiplexing; Biopuces; 530

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Vindas Yassine, K. (2017). Résonance plasmon et développements instrumentaux vers la conception de biopuces et biocapteurs innovants : Development and optimization of new plasmon resonance based biochips and biosensors. (Doctoral Dissertation). Université Grenoble Alpes (ComUE). Retrieved from http://www.theses.fr/2017GREAY091

Chicago Manual of Style (16^th Edition):

Vindas Yassine, Karim. “Résonance plasmon et développements instrumentaux vers la conception de biopuces et biocapteurs innovants : Development and optimization of new plasmon resonance based biochips and biosensors.” 2017. Doctoral Dissertation, Université Grenoble Alpes (ComUE). Accessed January 18, 2021. http://www.theses.fr/2017GREAY091.

MLA Handbook (7^th Edition):

Vindas Yassine, Karim. “Résonance plasmon et développements instrumentaux vers la conception de biopuces et biocapteurs innovants : Development and optimization of new plasmon resonance based biochips and biosensors.” 2017. Web. 18 Jan 2021.

Vancouver:

Vindas Yassine K. Résonance plasmon et développements instrumentaux vers la conception de biopuces et biocapteurs innovants : Development and optimization of new plasmon resonance based biochips and biosensors. [Internet] [Doctoral dissertation]. Université Grenoble Alpes (ComUE); 2017. [cited 2021 Jan 18]. Available from: http://www.theses.fr/2017GREAY091.

Council of Science Editors:

Vindas Yassine K. Résonance plasmon et développements instrumentaux vers la conception de biopuces et biocapteurs innovants : Development and optimization of new plasmon resonance based biochips and biosensors. [Doctoral Dissertation]. Université Grenoble Alpes (ComUE); 2017. Available from: http://www.theses.fr/2017GREAY091

Virginia Tech

24. Adducci, Benjamin Augustus. Detection of a Surrogate Biological Threat Agent (Bacillus globigii) with a Portable Surface Plasmon Resonance Biosensor.

Degree: MS, Plant Pathology, Physiology, and Weed Science, 2015, Virginia Tech

URL: http://hdl.handle.net/10919/73535

► New methods and technology are needed to detect biological agents that threaten the health of humans and domestic animals. The bacterium Bacillus anthracis, causal agent… (more)

Subjects/Keywords: anthrax; Bacillus anthracis; bacteria; biosecurity; biosensor; detection; pathogen; SPR; pathogen; spore; SPR

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Adducci, B. A. (2015). Detection of a Surrogate Biological Threat Agent (Bacillus globigii) with a Portable Surface Plasmon Resonance Biosensor. (Masters Thesis). Virginia Tech. Retrieved from http://hdl.handle.net/10919/73535

Chicago Manual of Style (16^th Edition):

Adducci, Benjamin Augustus. “Detection of a Surrogate Biological Threat Agent (Bacillus globigii) with a Portable Surface Plasmon Resonance Biosensor.” 2015. Masters Thesis, Virginia Tech. Accessed January 18, 2021. http://hdl.handle.net/10919/73535.

MLA Handbook (7^th Edition):

Adducci, Benjamin Augustus. “Detection of a Surrogate Biological Threat Agent (Bacillus globigii) with a Portable Surface Plasmon Resonance Biosensor.” 2015. Web. 18 Jan 2021.

Vancouver:

Adducci BA. Detection of a Surrogate Biological Threat Agent (Bacillus globigii) with a Portable Surface Plasmon Resonance Biosensor. [Internet] [Masters thesis]. Virginia Tech; 2015. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/10919/73535.

Council of Science Editors:

Adducci BA. Detection of a Surrogate Biological Threat Agent (Bacillus globigii) with a Portable Surface Plasmon Resonance Biosensor. [Masters Thesis]. Virginia Tech; 2015. Available from: http://hdl.handle.net/10919/73535

Johannes Gutenberg Universität Mainz

25. Falk, Ann. Structural characterisation of tBLMs.

Degree: 2009, Johannes Gutenberg Universität Mainz

URL: http://ubm.opus.hbz-nrw.de/volltexte/2009/2020/

► Biological membranes are one of the vital key elements of life but are also highly complex architectures. Therefore, various model membrane systems have been developed… (more)

▼ Biological membranes are one of the vital key elements of life but are also highly complex architectures. Therefore, various model membrane systems have been developed to enable systematic investigations of different membrane related processes. A biomimetic model architecture should provide a simplified system, which allows for systematic investigation of the membrane while maintaining the essential membrane characteristics such as membrane fluidity or electrical sealing properties. This work has been focused on two complementary parts. In a first part, the behaviour of the whey protein ß-lactoglobulin (ßlg) at a membrane interface has been investigated. Protein-lipid interactions have been studied using Langmuir monolayers at the air-water interface and tethered bilayer lipid membranes. A combination of different surface analytical techniques such as surface plasmon spectroscopy, neutron reflectivity and electrochemical techniques allowed for a detailed analysis of the underlying processes. Those experiments showed that the protein adsorbed in native confirmation, slightly flattened, to hydrophobic monolayers. If hydrophilic bilayers with defects were present, ßlg penetrated the upper layer. Interactions with phospholipids were only observed if the protein was denatured beforehand. Experiments at the air-water interface showed a more rigid conformation of the protein at acidic pH compared to alkaline pH. In the second part of this work, the structure of different model membrane systems has been investigated. Solid supported membrane systems have been established as powerful biomimetic architectures, which allow for the systematic investigation of various membrane related processes. Additionally, these systems have been proposed for biosensing applications. Tethered bilayer lipid membranes (tBLMS) are one type of solid supported membranes. The structure of the anchor lipid that tethers the membrane to the solid support has a significant impact on the membrane properties. Especially the sub-membrane part, which is defined by the spacer group, is important for the biological activity of incorporated membrane proteins. Various anchor lipids have been synthesised with different spacer and anchor groups. An increase of the spacer length led to a direct increase of the water reservoir beneath the membrane. However, this elongation also resulted in an amplified roughness of the monolayer and subsequently to diminished mechanical and electrical bilayer qualities. Additionally, a cholesterol-spacer had been designed to modulate the membrane fluidity. Model membrane systems with additional cholesterol-spacer or upper bilayer leaflets with additional cholesterol also exhibited an increased water reservoir with only slightly diminished mechanical and electrical abilities. Both parts show that tBLMs are very effective model systems that can be applied as biomimetic platforms to study for example lipid-protein interactions. They also enable the incorporation of ion channels and allow for potential biosensing…

Subjects/Keywords: Phospholipide, beta-lactoglobulin, SPR, EIS, NR, BAM; phospholipids, beta-lactoglobulin, SPR, EIS, NR, BAM; Natural sciences and mathematics

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Falk, A. (2009). Structural characterisation of tBLMs. (Doctoral Dissertation). Johannes Gutenberg Universität Mainz. Retrieved from http://ubm.opus.hbz-nrw.de/volltexte/2009/2020/

Chicago Manual of Style (16^th Edition):

Falk, Ann. “Structural characterisation of tBLMs.” 2009. Doctoral Dissertation, Johannes Gutenberg Universität Mainz. Accessed January 18, 2021. http://ubm.opus.hbz-nrw.de/volltexte/2009/2020/.

MLA Handbook (7^th Edition):

Falk, Ann. “Structural characterisation of tBLMs.” 2009. Web. 18 Jan 2021.

Vancouver:

Falk A. Structural characterisation of tBLMs. [Internet] [Doctoral dissertation]. Johannes Gutenberg Universität Mainz; 2009. [cited 2021 Jan 18]. Available from: http://ubm.opus.hbz-nrw.de/volltexte/2009/2020/.

Council of Science Editors:

Falk A. Structural characterisation of tBLMs. [Doctoral Dissertation]. Johannes Gutenberg Universität Mainz; 2009. Available from: http://ubm.opus.hbz-nrw.de/volltexte/2009/2020/

Johannes Gutenberg Universität Mainz

26. Prasad, Janak. Sensing applications of biofunctionalised plasmonic gold nanoparticles.

Degree: 2015, Johannes Gutenberg Universität Mainz

URL: http://ubm.opus.hbz-nrw.de/volltexte/2015/4030/

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Plasmonische Metallnanopartikel bündeln, verstärken und beeinflussen Licht auf nanoskopischer Ebene. Diese grundlegende Eigenschaft kommt von koheränten, kollektiven Schwingungen der Leitungsbandelektronen, die von einfallendem Licht resonant… (more)

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Plasmonische Metallnanopartikel bündeln, verstärken und beeinflussen Licht auf nanoskopischer Ebene. Diese grundlegende Eigenschaft kommt von koheränten, kollektiven Schwingungen der Leitungsbandelektronen, die von einfallendem Licht resonant angeregt und lokalisierte Oberflächenplasmonenresonanz (LSPR) oder ‚Partikelplasmonen‘ genannt werden. Plasmonen in Metallnanopartikeln wurden bisher z.B. zur Erkennen von pathogenen Biomolekülen, bei der photothermischen Therapie und zur Verbesserung der Effizienz von Solarzellen verwendet. In dieser Arbeit werde ich meinen Fokus auf die Synthese und Funktionalisierung von Goldnanopartikeln zur Anwendung als Sensoren legen.rnrnKürzliche Verbesserungen in der nasschemischen Synthese haben zur Herstellung von Goldnanopartikel mit unterschiedlichen Formen und Größen geführt, die sich in ihren Sensoreigenschaften unterscheiden. Unter den unterschiedlichen Sensorgeometrien sind Goldnanostäbchen die bevorzugte Form zur Biomolekül-Sensorik durch LSPR. Nanostäbchen werden durch eine positiv geladene CTAB-Schicht stabilisiert, die Proteine bei neutralem pH-Wert anziehen kann. Die Adsorption und Desorption von Proteinen an der Nanopartikeloberfläche und damit die Bindungskinetiken von Proteinen kann auf Einzelmolekülebene erforscht werden. Ich zeige hier eine Studie mit hoher örtlicher und zeitlicher Auflösung um einzelne Bindungsereignisse von Fibronectin auf Goldnanostäbchen darzustellen.rnrnGoldnanostäbchen müssen mit spezifischen biologischen Erkennungselementen funktionalisiert werden um eine Analyterkennung oder Proteinwechselwirkung zu erreichen. Ich funktionalisiere Goldnanostäbchen mit kurzen DNA-Sequenzen (Aptamer-Sequenzen und NTA konjugierten Polihymidinen) und habe anhand diese unterschiedlich sensitiven Partikel eine Studie mit verschiedenen Analyten (oder Protein-Protein Wechselwirkungen) erfolgreich durchgeführt.rn rnPlasmonen von Nanopartikel-Clustern koppeln miteinander, was ihre Resonanzenergie ändert. Der kontrollierte Zusammenbau von Nanopartikeln zu Dimeren oder höher geordneten Strukturen wie ‚Core-Satellites‘ können dazu dienen ihre Sensitivität zu erhöhen. Diese Cluster bieten eine hohe Sensitivität auf Grund der Anwesenheit von plasmonischen Hotspots in der Lücke zwischen zwei Partikeln. Die Plasmonkopplung ist ein Phänomen, das abhängig vom Abstand zweier Partikel zueinander ist und bildet somit die Basis von sogenannten Plasmon-Linealen. Ich habe eine Strategie entwickelt um Dimere aus Hsp90 funktionalisierten Goldnanosphären zu bilden. Diese Technik wird nicht durch Ausbleichen oder das Blinken von Farbstoffen limitiert und ich zeige zum ersten Mal wie man dadurch dynamische Proteinkonformationen untersuchen kann.rn

Plasmonic metal nanoparticles focus, amplify and manipulate light at the nanoscale level. This fundamental property comes from the collective coherent oscillations of conduction band electrons resonantly excited by incident light in metal nanoparticles called Localised Surface Plasmon Resonance (LSPR) or ‘particle plasmons’. Plasmons in metal…

Subjects/Keywords: Plasmonen; Einzel Partikel Spektroskopie; Gold Nanopartikel, Sensoren; SPR; Plasmons; Single Particle Spectroscopy; Gold Nanoparticles; Sensors; SPR; Chemistry and allied sciences

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Prasad, J. (2015). Sensing applications of biofunctionalised plasmonic gold nanoparticles. (Doctoral Dissertation). Johannes Gutenberg Universität Mainz. Retrieved from http://ubm.opus.hbz-nrw.de/volltexte/2015/4030/

Chicago Manual of Style (16^th Edition):

Prasad, Janak. “Sensing applications of biofunctionalised plasmonic gold nanoparticles.” 2015. Doctoral Dissertation, Johannes Gutenberg Universität Mainz. Accessed January 18, 2021. http://ubm.opus.hbz-nrw.de/volltexte/2015/4030/.

MLA Handbook (7^th Edition):

Prasad, Janak. “Sensing applications of biofunctionalised plasmonic gold nanoparticles.” 2015. Web. 18 Jan 2021.

Vancouver:

Prasad J. Sensing applications of biofunctionalised plasmonic gold nanoparticles. [Internet] [Doctoral dissertation]. Johannes Gutenberg Universität Mainz; 2015. [cited 2021 Jan 18]. Available from: http://ubm.opus.hbz-nrw.de/volltexte/2015/4030/.

Council of Science Editors:

Prasad J. Sensing applications of biofunctionalised plasmonic gold nanoparticles. [Doctoral Dissertation]. Johannes Gutenberg Universität Mainz; 2015. Available from: http://ubm.opus.hbz-nrw.de/volltexte/2015/4030/

27. Bornert, Olivier. Caractérisation moléculaire et structurale de la famille des protéines GASP : Molecular and structural characterization of the GASP family of proteins.

Degree: Docteur es, Aspects moléculaires et cellulaires de la biologie, 2013, Université de Strasbourg

URL: http://www.theses.fr/2013STRAJ033

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Les RCPG sont exprimés dans tous types de tissus et sont impliqués dans la régulation de nombreux processus biologiques et ont pour rôle de capter… (more)

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Les RCPG sont exprimés dans tous types de tissus et sont impliqués dans la régulation de nombreux processus biologiques et ont pour rôle de capter un vaste panel de stimuli extracellulaires qu’ils transmettent à l’intérieur de la cellule. Récemment, le laboratoire a identifié une nouvelle famille de dix protéines, les GASP, qui interagissent avec les RCPG et moduleraient leur trafic intracellulaire. Alors que GASP-1 est le membre de cette famille le mieux caractérisé et que son interaction avec de nombreux RCPG soit documentée, peu d’informations sont disponibles sur les modalités d’interaction de cette protéineavec les RCPG au niveau moléculaire. La première partie de ce projet de thèse a consisté à étudier les modalités d’interaction entre les GASPs et les RCPG au niveau moléculaire. Nous avons ainsi pu montrer à l’aide de techniques biochimiques et biophysiques, l’importance d’un motif répété et conservé de 15 acides aminés pour l’interaction de GASP-1 avec divers RCPG. Par la suite, les résultats obtenus ont été exploités pour mettre en place un essai de criblage qui nous a permis d’identifier des petites molécules capables de perturber l’interaction entre GASP-1 et le récepteur beta-2 adrénergique. Enfin, l’absence de données structurales sur les protéines de la famille GASP nous a ensuite poussé à la réalisation d’études structurales de ces protéines à la fois par cristallographie et par RMN. Bien que les résultats obtenus ne nous aient pas encore permis d’obtenir la structure de ces protéines, des expériences préliminaires de RMN ont permis deconfirmer l’implication des acides aminés tryptophanes présents au sein des motifs GASP dans l’interaction avec les RCPG.

GPCRs represent one of the most diversified protein families in humans. They translate extracellular stimuli into intracellular signals to modulate a large panel of physiological processes making them unrivalled targets for development of new therapeutic agents. Recently, we identified the GASP family of proteins that interact with GPCRs and modulate the postendocyticfate of agonist activated receptors. GASP-1 is the well-characterized protein of this family and has been shown to be involved in the sorting of receptors that are quickly degraded following agonistpromoted internalization. Although GASP-1 was found to interact with numerous GPCR both in vitro and in vivo and that helix 8 of GPCRs is critically involved in this interaction, little is known about which region within GASP-1 is required for its interaction with GPCRs. In this work, we first present a detailed analysis of the molecular interaction between GASPs and GPCRs. By using biochemical and biophysical experiments we shown that the central domain of GASP-1 is critical for the interaction with GPCRs and that a conserved and repeated sequence of 15 amino acids plays a critical role in this interaction. In a second step, we developed an HTC assay allowing us to identify small molecules able to disrupt the interaction between GASP-1 and the beta-2 adrenergic receptor. Finally, preliminary…

Advisors/Committee Members: Simonin, Frédéric (thesis director).

Subjects/Keywords: RCPG; Interaction protéine-protéine; Protéine membranaire; SPR; GASP; GPCR; Protein-protein interaction; Membrane protein; SPR; GASP; 572.6; 572.8

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bornert, O. (2013). Caractérisation moléculaire et structurale de la famille des protéines GASP : Molecular and structural characterization of the GASP family of proteins. (Doctoral Dissertation). Université de Strasbourg. Retrieved from http://www.theses.fr/2013STRAJ033

Chicago Manual of Style (16^th Edition):

Bornert, Olivier. “Caractérisation moléculaire et structurale de la famille des protéines GASP : Molecular and structural characterization of the GASP family of proteins.” 2013. Doctoral Dissertation, Université de Strasbourg. Accessed January 18, 2021. http://www.theses.fr/2013STRAJ033.

MLA Handbook (7^th Edition):

Bornert, Olivier. “Caractérisation moléculaire et structurale de la famille des protéines GASP : Molecular and structural characterization of the GASP family of proteins.” 2013. Web. 18 Jan 2021.

Vancouver:

Bornert O. Caractérisation moléculaire et structurale de la famille des protéines GASP : Molecular and structural characterization of the GASP family of proteins. [Internet] [Doctoral dissertation]. Université de Strasbourg; 2013. [cited 2021 Jan 18]. Available from: http://www.theses.fr/2013STRAJ033.

Council of Science Editors:

Bornert O. Caractérisation moléculaire et structurale de la famille des protéines GASP : Molecular and structural characterization of the GASP family of proteins. [Doctoral Dissertation]. Université de Strasbourg; 2013. Available from: http://www.theses.fr/2013STRAJ033

Université de Grenoble

28. Melaine, Feriel. Biopuce à aptamères : application à la détection de petites molécules par imagerie de résonnance plasmonique de surface : Aptasensor for small molecules detection using surface plasmon resonance imaging.

Degree: Docteur es, Physique pour les sciences du vivant, 2014, Université de Grenoble

URL: http://www.theses.fr/2014GRENY054

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Les aptamères correspondent à de courtes séquences d'oligonucléotides possédant une forte affinité et spécificité envers un ligand (petites molécules organiques, peptides, acides nucléiques, protéines, cellules).… (more)

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Les aptamères correspondent à de courtes séquences d'oligonucléotides possédant une forte affinité et spécificité envers un ligand (petites molécules organiques, peptides, acides nucléiques, protéines, cellules). Du fait de leurs remarquables propriétés, ils sont utilisés comme alternative aux anticorps dans les dispositifs de type biocapteur/biopuce, notamment pour la détection de petites molécules (PM < 2000 Da). L'imagerie de résonance des plasmons de surface (SPRi) est une technique de détection optique qui a gagné une attention croissante ces dernières années. Elle est basée sur un principe de variation de l'indice de réfraction d'une surface sélective lors de l'interaction sonde/cible. Sa sensibilité est néanmoins limitée aux molécules de poids moléculaire supérieur à 2000 Da. Dans le cadre de ces travaux, nous avons développé une biopuce à aptamères pour à la détection d'une petite molécule, l'adénosine, au moyen de la technique de résonance des plasmons de surface (SPR). Pour cela, deux différentes stratégies ont été développées. La première combine l'utilisation de nanoparticules d'or (AuNPs) pour l'amplification du signal SPRi avec l'ingénierie des séquences d'aptamères. La seconde stratégie est basée sur l'exploitation de la stabilité thermodynamique apportée par l'interaction de la cible (adénosine) avec les séquences d'aptamères. Le dispositif SPR est alors couplé à un système de régulation de température, permettant ainsi d'assurer la dissociation des complexes et d'établir des profils de dénaturation caractéristiques. Nos résultats initient ainsi une nouvelle approche dans la détection de petites molécules par SPRi et ouvrent de nouvelles perspectives de développement des biocapteurs à aptamères.

Aptamers are single-stranded DNA (ssDNA) or RNA molecules capable of binding to target molecules, including proteins, metal ions and drugs. Because of their specific binding abilities and many advantages over antibodies (higher stability, lower cost, easy chemical modification…), they provide a great opportunity to produce sensing surfaces for effective and selective detection of small molecules. Surface Plasmon Resonance imaging (SPRi) has become one of the most widely used label-free method for the study of biorecognition events on sensor surfaces. This technique provides a rapid approach, however, limited by low refractive index changes occurring when small molecules (<2000 Da) are captured on the sensor. Whereas significant reflectivity variation is observed upon the interaction of large molecules like proteins with the sensing interface, for small molecules targets such adenosine, the reflectivity variation is often too small to be detected by SPRi. Thereby, only few studies have been reported so far on SPRi-based biosensor for small molecules detection using aptamers. In this work, we developed two bioassay strategies for the detection of a model small molecule, adenosine, using Surface Plasmon Resonance imaging. The first one combines the SPRi signal enhancement effect induced by gold nanoparticles…

Advisors/Committee Members: Buhot, Arnaud (thesis director), Roupioz, Yoann (thesis director).

Subjects/Keywords: Biopuces; Nanoparticles d'Or; SPR; Petites molécules; Aptamères; Courbes de dénaturation; Aptasensor; Gold Nanoparticles; SPR; Small molecules; Aptamers; Melting curves; 530

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Melaine, F. (2014). Biopuce à aptamères : application à la détection de petites molécules par imagerie de résonnance plasmonique de surface : Aptasensor for small molecules detection using surface plasmon resonance imaging. (Doctoral Dissertation). Université de Grenoble. Retrieved from http://www.theses.fr/2014GRENY054

Chicago Manual of Style (16^th Edition):

Melaine, Feriel. “Biopuce à aptamères : application à la détection de petites molécules par imagerie de résonnance plasmonique de surface : Aptasensor for small molecules detection using surface plasmon resonance imaging.” 2014. Doctoral Dissertation, Université de Grenoble. Accessed January 18, 2021. http://www.theses.fr/2014GRENY054.

MLA Handbook (7^th Edition):

Melaine, Feriel. “Biopuce à aptamères : application à la détection de petites molécules par imagerie de résonnance plasmonique de surface : Aptasensor for small molecules detection using surface plasmon resonance imaging.” 2014. Web. 18 Jan 2021.

Vancouver:

Melaine F. Biopuce à aptamères : application à la détection de petites molécules par imagerie de résonnance plasmonique de surface : Aptasensor for small molecules detection using surface plasmon resonance imaging. [Internet] [Doctoral dissertation]. Université de Grenoble; 2014. [cited 2021 Jan 18]. Available from: http://www.theses.fr/2014GRENY054.

Council of Science Editors:

Melaine F. Biopuce à aptamères : application à la détection de petites molécules par imagerie de résonnance plasmonique de surface : Aptasensor for small molecules detection using surface plasmon resonance imaging. [Doctoral Dissertation]. Université de Grenoble; 2014. Available from: http://www.theses.fr/2014GRENY054

29. Boulade, Marine. Imagerie SPR optimisée en résolution pour l'étude et la détection de bactéries : Resolution optimized SPR imaging for the study and detection of bacteria.

Degree: Docteur es, Biotechnologie, instrumentation, signal et imagerie pour la biologie, la médecine et l'environnement, 2019, Université Grenoble Alpes (ComUE); Université de Sherbrooke (Québec, Canada)

URL: http://www.theses.fr/2019GREAS004

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L’étude, la détection et l’identification de pathogènes est une problématique majeure pour la sécurité alimentaire et la médecine. Cependant, les pathogènes bactériens présents à de… (more)

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L’étude, la détection et l’identification de pathogènes est une problématique majeure pour la sécurité alimentaire et la médecine. Cependant, les pathogènes bactériens présents à de faibles concentrations nécessitent souvent une période de plus de 36h pour être identifiés par les méthodes standards. Ce délai est extrêmement contraignant pour des domaines où la rapidité du diagnostic est un facteur clé. Il y a donc une forte demande pour le développement d’outils pour mieux comprendre le comportement bactérien et ainsi développer des techniques de détection plus rapides et plus performantes.Les systèmes d’imagerie SPR sont largement utilisés pour l’analyse d’interactions moléculaires, car ils permettent une mesure en parallèle, en temps réel et sans marquage, tout en étant faciles d’utilisation et compatibles avec des milieux complexes. Cette technique a montré son efficacité pour l'étude et la détection de bactéries en utilisant les interactions moléculaires avec les anticorps, mais les délais de détection restent pénalisants.Dans ce contexte, un nouveau système d’imagerie permettant l’étude et la détection spécifique de pathogènes bactériens performant est développé en mettant à profit les avancées récentes en imagerie SPR optimisée en résolution. Notre système permet d'améliorer les temps de détection de pathogènes en milieux modèles grâce à sa capacité à détecter des bactéries individuelles. Il peut également être utilisé pour l'étude de l'interaction entre bactéries et surfaces spécifiques. Des premiers tests montrent que notre instrument est capable de caractériser le comportement bactérien de plusieurs souches bactériennes en interaction avec des surfaces fonctionnalisées par des espèces chimiques différentes

The study, detection and identification of pathogens is a major issue for food safety and medicine. However, bacterial pathogens present at low concentrations often require a period of more than 36 hours to be identified by standard methods. This delay is extremely constraining for areas where rapid diagnosis is a key factor. There is therefore a strong demand for the development of tools to better understand bacterial behavior and thus develop faster and more efficient detection techniques.SPR imaging systems are widely used for the analysis of molecular interactions, as they allow parallel, real-time and unlabeled measurement, while being easy to use and compatible with complex media. This technique has proven effective in the study and detection of bacteria using molecular interactions with antibodies, but detection times remain penalizing.In this context, a new imaging system allowing the study and specific detection of high-performance bacterial pathogens is being developed, taking advantage of recent advances in SPR imaging optimized in resolution. Our system improves pathogen detection times in model environments through its ability to detect individual bacteria. It can also be used to study the interaction between bacteria and specific surfaces. Initial tests show that our instrument is capable…

Advisors/Committee Members: Livache, Thierry (thesis director), Charette, Paul (thesis director), Leroy, Loïc (thesis director), Canva, Michael (thesis director).

Subjects/Keywords: Biocapteurs; Détection de pathogènes; Bactéries; Traitement d'image; Spr; Biosensors; Pathogen detection; Bacteria; Image processing; Spr; 610

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Boulade, M. (2019). Imagerie SPR optimisée en résolution pour l'étude et la détection de bactéries : Resolution optimized SPR imaging for the study and detection of bacteria. (Doctoral Dissertation). Université Grenoble Alpes (ComUE); Université de Sherbrooke (Québec, Canada). Retrieved from http://www.theses.fr/2019GREAS004

Chicago Manual of Style (16^th Edition):

Boulade, Marine. “Imagerie SPR optimisée en résolution pour l'étude et la détection de bactéries : Resolution optimized SPR imaging for the study and detection of bacteria.” 2019. Doctoral Dissertation, Université Grenoble Alpes (ComUE); Université de Sherbrooke (Québec, Canada). Accessed January 18, 2021. http://www.theses.fr/2019GREAS004.

MLA Handbook (7^th Edition):

Boulade, Marine. “Imagerie SPR optimisée en résolution pour l'étude et la détection de bactéries : Resolution optimized SPR imaging for the study and detection of bacteria.” 2019. Web. 18 Jan 2021.

Vancouver:

Boulade M. Imagerie SPR optimisée en résolution pour l'étude et la détection de bactéries : Resolution optimized SPR imaging for the study and detection of bacteria. [Internet] [Doctoral dissertation]. Université Grenoble Alpes (ComUE); Université de Sherbrooke (Québec, Canada); 2019. [cited 2021 Jan 18]. Available from: http://www.theses.fr/2019GREAS004.

Council of Science Editors:

Boulade M. Imagerie SPR optimisée en résolution pour l'étude et la détection de bactéries : Resolution optimized SPR imaging for the study and detection of bacteria. [Doctoral Dissertation]. Université Grenoble Alpes (ComUE); Université de Sherbrooke (Québec, Canada); 2019. Available from: http://www.theses.fr/2019GREAS004

30. Alvarado- Meza, Ricardo. Stratégies de fonctionnalisation pour le développement de biopuces innovantes : Functionalization strategies for the development of innovative biochips.

Degree: Docteur es, Chimie biologie, 2018, Université Grenoble Alpes (ComUE)

URL: http://www.theses.fr/2018GREAV030

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Une pléthore de processus biologiquement pertinents dépend directement de la sécrétion de biomolécules dans le milieu extracellulaire, aux fonctions régulatrices ou composants structurels. L’analyse de… (more)

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Une pléthore de processus biologiquement pertinents dépend directement de la sécrétion de biomolécules dans le milieu extracellulaire, aux fonctions régulatrices ou composants structurels. L’analyse de processus biologiques complexes nécessite ainsi la mise au point de nouveaux outils de biodétection. Par conséquent, le but de cette thèse est de fournir des stratégies polyvalentes pour la génération de biocapteurs et de biopuces innovants basés sur la Résonance des Plasmons de Surface (SPR). À l’issue de ces travaux, une méthode de photofonctionnalisation indirecte a été mise au point. Ce procédé a permis de générer des micro-réseaux de protéines dans des conditions entièrement aqueuses, et ainsi de préserver la fonctionnalité des protéines greffées. De plus, nous avons créé et évalué une nouvelle biopuce SPR microstructurée pour le suivi en temps réel des sécrétions cellulaires. Cette biopuce microstructurée présente deux phénomènes optiques différents qui peuvent être utilisés pour la détection cellulaire et le suivi de leurs sécrétions. Enfin, de multiples stratégies de fonctionnalisation ont été évaluées pour la conception d’une biopuce SPR nanostructurée à faisceau de fibres optiques. Parmi ces approches, la génération de monocouches photoréactives auto-assemblées a été la plus adaptée à ce système et est en cours d’optimisation. Une fois réalisée, cette biopuce nanostructurée pourrait ouvrir la voie à la poursuite du développement de systèmes prometteurs de biodétection in vivo.

A plethora of biologically relevant processes depends directly on the effective secretion of biomolecules, from regulatory molecules to structural components. Thus, the analysis of complex biological processes requires the development of novel biosensing tools. Therefore, the aim of this thesis is to provide versatile strategies for the generation of innovative biosensors and biochips based on Surface Plasmon Resonance (SPR). As a result from this research, an indirect photofunctionalization method was developed. This procedure allowed the generation of protein microarrays in fully aqueous conditions while preserving the functionality of the grafted proteins. Furthermore, we created and evaluated a novel microstructured SPR biochip for real-time monitoring of cellular secretions. This microstructured biochip presents two different optical phenomena which could be used for cell detection and the monitoring of their secretions. Finally, multiple functionalization strategies were evaluated for the conception of a nanostructured fiber-bundle SPR biochip. Among the approaches, the generation of photoreactive self-assembled monolayers was the most adapted to this system and currently is being optimized. Once achieved, this nanostructured biochip could pave the way for further development of promising in vivo biosensing systems.

Advisors/Committee Members: Roupioz, Yoann (thesis director), Leroy, Loïc (thesis director).

Subjects/Keywords: Biopuce; Chimie de surface; Cellules; Réponse immunitaire; Spr; Micro-Array; Surface chemistry; Cells; Biochip; Spr; 570; 540

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Alvarado- Meza, R. (2018). Stratégies de fonctionnalisation pour le développement de biopuces innovantes : Functionalization strategies for the development of innovative biochips. (Doctoral Dissertation). Université Grenoble Alpes (ComUE). Retrieved from http://www.theses.fr/2018GREAV030

Chicago Manual of Style (16^th Edition):

Alvarado- Meza, Ricardo. “Stratégies de fonctionnalisation pour le développement de biopuces innovantes : Functionalization strategies for the development of innovative biochips.” 2018. Doctoral Dissertation, Université Grenoble Alpes (ComUE). Accessed January 18, 2021. http://www.theses.fr/2018GREAV030.

MLA Handbook (7^th Edition):

Alvarado- Meza, Ricardo. “Stratégies de fonctionnalisation pour le développement de biopuces innovantes : Functionalization strategies for the development of innovative biochips.” 2018. Web. 18 Jan 2021.

Vancouver:

Alvarado- Meza R. Stratégies de fonctionnalisation pour le développement de biopuces innovantes : Functionalization strategies for the development of innovative biochips. [Internet] [Doctoral dissertation]. Université Grenoble Alpes (ComUE); 2018. [cited 2021 Jan 18]. Available from: http://www.theses.fr/2018GREAV030.

Council of Science Editors:

Alvarado- Meza R. Stratégies de fonctionnalisation pour le développement de biopuces innovantes : Functionalization strategies for the development of innovative biochips. [Doctoral Dissertation]. Université Grenoble Alpes (ComUE); 2018. Available from: http://www.theses.fr/2018GREAV030

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Open Access Theses and Dissertations