subject:(Spinach)

Technical University of Lisbon

1. Couto, Cristina Efimovna do. Chlorophyll and green color stabilization on vegetable homogenates.

Degree: 2016, Technical University of Lisbon

URL: https://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/12924

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Mestrado em Engenharia Alimentar - Instituto Superior de Agronomia - UL

Several factors influence chlorophyll stability in foods and beverages. This study aims to determine… (more)

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Mestrado em Engenharia Alimentar - Instituto Superior de Agronomia - UL

Several factors influence chlorophyll stability in foods and beverages. This study aims to determine the influence of raw material, heat treatment, pH and food additives on chlorophyll content and color stability of vegetable homogenates. Fully expanded spinach, parsley, broccoli, and lettuce leaves were harvested and immediately processed. Leaf (10%) homogenates were bottled, heat-treated at 90 ºC for 60s, cooled and stored at 20 ºC. Chlorophylls a and b were measured before and after pasteurization, and during shelf-life. Color was assessed with a colorimeter. Broccoli and parsley, spinach and lettuce had an initial hue angles of 132.8°, 129.0° and 109.8°, respectively. Chlorophyll content was higher in broccoli (171.4 μg mL-1) and lower in lettuce (44.4 μg mL-1). Hue and chlorophyll content decreased in all homogenates during pasteurization. At pH 8.5 the addition of sodium bicarbonate and zinc chloride had a positive effect on retention of chlorophyll and hue of spinach, parsley and broccoli homogenates, with hue increases of 8% in samples treated with sodium bicarbonate and 9% in those with zinc chloride. At pH 4.5 hue decreased 18% in all samples, with accompanying color shift to olive green. During shelf-life, hue and chlorophyll content was better maintained at higher pH in all samples, with higher values registered for broccoli samples, irrespective of additive. Pre-harvest zinc application in lettuce had a positive effect on color of pasteurized samples, with no significant effect in chlorophyll content. However, there was visual evidence of color retention for these samples. Total chlorophylls decreased 19% in all lettuce samples due to heat treatment. While hue, chlorophyll content and visual color were better maintained in samples with pre-harvest treatments during shelflife, the addition of zinc did not affect these parameters. A significant relationship between sample hue and total chlorophyll content was found, for all vegetable samples in study

N/A

Advisors/Committee Members: Almeida, Domingos Paulo Ferreira, Alegria, Maria João.

Subjects/Keywords: brocolli; color; lettuce; parsley; spinach

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APA (6^th Edition):

Couto, C. E. d. (2016). Chlorophyll and green color stabilization on vegetable homogenates. (Thesis). Technical University of Lisbon. Retrieved from https://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/12924

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Couto, Cristina Efimovna do. “Chlorophyll and green color stabilization on vegetable homogenates.” 2016. Thesis, Technical University of Lisbon. Accessed April 11, 2021. https://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/12924.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Couto, Cristina Efimovna do. “Chlorophyll and green color stabilization on vegetable homogenates.” 2016. Web. 11 Apr 2021.

Vancouver:

Couto CEd. Chlorophyll and green color stabilization on vegetable homogenates. [Internet] [Thesis]. Technical University of Lisbon; 2016. [cited 2021 Apr 11]. Available from: https://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/12924.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Couto CEd. Chlorophyll and green color stabilization on vegetable homogenates. [Thesis]. Technical University of Lisbon; 2016. Available from: https://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/12924

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Alberta

2. Vose, John Randal. Studies on energy dependent reactions in isolated chloroplasts.

Degree: PhD, Department of Plant Science, 1968, University of Alberta

URL: https://era.library.ualberta.ca/files/76537348k

Subjects/Keywords: Spinach.; Chloroplasts.

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Vose, J. R. (1968). Studies on energy dependent reactions in isolated chloroplasts. (Doctoral Dissertation). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/76537348k

Chicago Manual of Style (16^th Edition):

Vose, John Randal. “Studies on energy dependent reactions in isolated chloroplasts.” 1968. Doctoral Dissertation, University of Alberta. Accessed April 11, 2021. https://era.library.ualberta.ca/files/76537348k.

MLA Handbook (7^th Edition):

Vose, John Randal. “Studies on energy dependent reactions in isolated chloroplasts.” 1968. Web. 11 Apr 2021.

Vancouver:

Vose JR. Studies on energy dependent reactions in isolated chloroplasts. [Internet] [Doctoral dissertation]. University of Alberta; 1968. [cited 2021 Apr 11]. Available from: https://era.library.ualberta.ca/files/76537348k.

Council of Science Editors:

Vose JR. Studies on energy dependent reactions in isolated chloroplasts. [Doctoral Dissertation]. University of Alberta; 1968. Available from: https://era.library.ualberta.ca/files/76537348k

University of Nairobi

3. Osano, AA. Spinach chlorotic spot virus: a new strain of 8eet yellows virus .

Degree: 1985, University of Nairobi

URL: http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/27100

► A Uirus causing systemic chlorotic spot symptom in spinach (Soinacea oleracea) was characterized and iden tified as a strain of beet yellows virus and was… (more)

▼ A Uirus causing systemic chlorotic spot symptom in spinach (Soinacea oleracea) was characterized and iden tified as a strain of beet yellows virus and was referred to as spinach chlorotic spot virus (SCSU). The virus particles were flexuous and approximately 840 nm in length. In glasshouse all the beet varieties (Crimson globe, Eclipe and Detroit) and spinach varieties (lucullus, Foordhook giant and Swiss chard) tested were susceptible. The virus isolate was limited in host range to the family Chenopodiaceae. In Beta vulgaris. a member of chenopodiaceae family, systemic necrotic lesions were observed about 8-10 days after inoculation. The necrosis was more marked in mature leaves and there was general intensification of red colour. A few leaves showed yellow spots and the plants were also stunted. Necrotic lesions, 2-3 mm in diameter, were also observed in Chenopodi tim amaranti col or 5 days after inoculation. Systemic tip necrosis was also observed 2-3 weeks after inoculation. Chenopodi urn gui noa and C. album showed the same type of symptoms already described for C. amaranti col or. White necrotic spots, about 2-3 nm in diameter, with reddish outline, observed in Gomphrery xi i i globosa. Chenopodium amaranticolor was used as an assay host. SCSU was successfuly transmitted in a semi persistent manner by the aphid Myzus persicae. The virus isolate had a thermal inactivation point (TIP) at o o -4 56 C and not 54 C; dilution end-point (DEP at 10 and -5 not 10 i longevity in in vitro (LIU) at one days and not 2 days. SCSU was partially purified from infected spinach leaves by differential centrifugation. The virus was extracted with 0.1M phosphate buffer, pH 7.2, and clarified in 72 ether. In homologous reactions, the virus reacted with its own antiserum to the titre of 1/1024 in nii cropreci pi ti n tests. On the basis of these criteria and results obtained, the virus inducing chlorotic spots in spinach was identified as a strain of beet yellows virus.

Subjects/Keywords: Spinach chlorotic

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APA (6^th Edition):

Osano, A. (1985). Spinach chlorotic spot virus: a new strain of 8eet yellows virus . (Thesis). University of Nairobi. Retrieved from http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/27100

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Osano, AA. “Spinach chlorotic spot virus: a new strain of 8eet yellows virus .” 1985. Thesis, University of Nairobi. Accessed April 11, 2021. http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/27100.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Osano, AA. “Spinach chlorotic spot virus: a new strain of 8eet yellows virus .” 1985. Web. 11 Apr 2021.

Vancouver:

Osano A. Spinach chlorotic spot virus: a new strain of 8eet yellows virus . [Internet] [Thesis]. University of Nairobi; 1985. [cited 2021 Apr 11]. Available from: http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/27100.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Osano A. Spinach chlorotic spot virus: a new strain of 8eet yellows virus . [Thesis]. University of Nairobi; 1985. Available from: http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/27100

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Washington State University

4. [No author]. Management of Fusarium wilt in spinach seed crops in the maritime Pacific Northwest USA .

Degree: 2013, Washington State University

URL: http://hdl.handle.net/2376/4957

► The maritime Pacific Northwest is the only region of the USA suitable for production of spinach seed, a cool-season, daylength-sensitive crop. However, the acidic soils… (more)

▼ The maritime Pacific Northwest is the only region of the USA suitable for production of spinach seed, a cool-season, daylength-sensitive crop. However, the acidic soils of this region are highly conducive to spinach Fusarium wilt, caused by Fusarium oxysporum f. sp. spinaciae. Rotations of 10 to 15 years between spinach seed crops are necessary to reduce the risk of losses to this disease. Raising soil pH with limestone partially suppresses spinach Fusarium wilt, but the suppressive effect is transitory, and the disease still limits seed crop acreage in the region. Experiments were conducted to: 1) assess the potential for annual applications of limestone for three years prior to a spinach seed crop to improve Fusarium wilt suppression compared to the level of suppression from a single limestone amendment, 2) develop a soil-based greenhouse bioassay to characterize the spinach Fusarium wilt risk of soil samples submitted from stakeholders' fields, and 3) explore the mechanism(s) of limestone-mediated Fusarium wilt suppression. Annual applications of limestone for each of three years prior to a spinach seed crop were superior to a single limestone application for suppressing Fusarium wilt and increasing seed yield. A soil bioassay to assess Fusarium wilt risk was developed in which three spinach lines representing a range in Fusarium wilt susceptibility were used to test soil samples from growers' fields under consideration for spinach seed crops. In the four years that the soil bioassay has been offered as a risk assessment service, soil samples from 147 fields were submitted by stakeholders for evaluation. Follow-up visits to spinach seed crops planted in fields assessed with the soil bioassay validated the results. In vitro experiments demonstrated that deficiencies of iron, manganese, and zinc can reduce growth and sporulation of F. oxysporum f. sp. spinaciae. Furthermore, greenhouse experiments in naturally-infested field soil indicated that reduction in availability of these micronutrients in limestone-amended soils reduced Fusarium wilt inoculum potential. Together, these findings reveal relationships among soil properties and spinach Fusarium wilt development, increase the capacity for and profitability of USA spinach seed production, and will guide future research on soil-based management of this disease. Advisors/Committee Members: Du Toit, Lindsey (advisor).

Subjects/Keywords: Plant pathology; Fusarium wilt; Spinach

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

author], [. (2013). Management of Fusarium wilt in spinach seed crops in the maritime Pacific Northwest USA . (Thesis). Washington State University. Retrieved from http://hdl.handle.net/2376/4957

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

author], [No. “Management of Fusarium wilt in spinach seed crops in the maritime Pacific Northwest USA .” 2013. Thesis, Washington State University. Accessed April 11, 2021. http://hdl.handle.net/2376/4957.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

author], [No. “Management of Fusarium wilt in spinach seed crops in the maritime Pacific Northwest USA .” 2013. Web. 11 Apr 2021.

Vancouver:

author] [. Management of Fusarium wilt in spinach seed crops in the maritime Pacific Northwest USA . [Internet] [Thesis]. Washington State University; 2013. [cited 2021 Apr 11]. Available from: http://hdl.handle.net/2376/4957.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

author] [. Management of Fusarium wilt in spinach seed crops in the maritime Pacific Northwest USA . [Thesis]. Washington State University; 2013. Available from: http://hdl.handle.net/2376/4957

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Illinois – Urbana-Champaign

5. Wadlington, William H. Characterization of the sex chromosomes in spinach.

Degree: PhD, Plant Biology, 2017, University of Illinois – Urbana-Champaign

URL: http://hdl.handle.net/2142/98172

► Plant sex chromosomes have evolved from the autosomes of hermaphroditic species to maintain dioecy. The major barriers to sex chromosome characterization have been the inherent… (more)

▼ Plant sex chromosomes have evolved from the autosomes of hermaphroditic species to maintain dioecy. The major barriers to sex chromosome characterization have been the inherent difficulties of working with a non-recombining sex determining region that make sequencing and mapping the Male-Specific region of the Y chromosome (MSY) painstaking. Here we use YY spinach (Spinacia oleracea L.) to characterize the MSY and the X-specific region of the X chromosome. A screen of 395 accessions in the USDA germplasm collection found a single accession that segregates YY progeny. The YY genotype was verified by a genetic cross and a novel X-specific marker. Potential novel X-specific sequences were found by depth of coverage analysis comparing alignments of male and female sequences to an XX reference. Of the 19 candidates found by depth of coverage, only one was verified as X-specific. The marker SpoX amplifies products from XX and XY but not YY templates. Pooled genomic DNA of 16 YY individuals selected by SpoX was sequenced at 63X using PacBio. Sequence data was assembled into an 823 Mbp assembly using CANU so the YY assembly could be compared to a 911 Mbp XX assembly. Seven genes that were non-repetitive sequences were found on a 1.14 X-specific contig from the XX assembly, and were queried by BLAST to the YY assembly to find their MSY homologs, which totaled 427 kb of novel Y chromosome sequence. On the Y contigs, the percentage uncovered by female k-mers decreased from 9.5% to 0.6% between positions 65.8 Mbp and 66.7 Mbp on the X chromosome, indicating that it is the location of one of the boundaries of the non-recombining region. The discovery of an accession which reliably segregates YY plants makes for unprecedented opportunity to study X and Y chromosomes in spinach. By comparing YY and XX genomes, the genomic basis of X and Y chromosome differentiation and evolution that gave rise to dioecy can be elucidated. Advisors/Committee Members: Ming, Ray (advisor), Ming, Ray (Committee Chair), Heath, Katy (committee member), Downie, Stephen (committee member), Tranel, Pat (committee member), Hanzawa, Yoshie (committee member).

Subjects/Keywords: Spinach; Sex chromosomes; Dioecy

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wadlington, W. H. (2017). Characterization of the sex chromosomes in spinach. (Doctoral Dissertation). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/98172

Chicago Manual of Style (16^th Edition):

Wadlington, William H. “Characterization of the sex chromosomes in spinach.” 2017. Doctoral Dissertation, University of Illinois – Urbana-Champaign. Accessed April 11, 2021. http://hdl.handle.net/2142/98172.

MLA Handbook (7^th Edition):

Wadlington, William H. “Characterization of the sex chromosomes in spinach.” 2017. Web. 11 Apr 2021.

Vancouver:

Wadlington WH. Characterization of the sex chromosomes in spinach. [Internet] [Doctoral dissertation]. University of Illinois – Urbana-Champaign; 2017. [cited 2021 Apr 11]. Available from: http://hdl.handle.net/2142/98172.

Council of Science Editors:

Wadlington WH. Characterization of the sex chromosomes in spinach. [Doctoral Dissertation]. University of Illinois – Urbana-Champaign; 2017. Available from: http://hdl.handle.net/2142/98172

Technical University of Lisbon

6. Pinto, Filipa Rego. Avaliação da resposta ao stresse oxidativo induzido por cádmio em plantas de espinafre Spinacea oleracea L.

Degree: 2011, Technical University of Lisbon

URL: http://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/4182

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Mestrado em Engenharia Agronómica - Instituto Superior de Agronomia

The main objective of this work was to evaluate the response of spinach plants to induced… (more)

Subjects/Keywords: Spinacea oleracea; spinach; cadmium; oxidative stress

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APA (6^th Edition):

Pinto, F. R. (2011). Avaliação da resposta ao stresse oxidativo induzido por cádmio em plantas de espinafre Spinacea oleracea L. (Thesis). Technical University of Lisbon. Retrieved from http://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/4182

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Pinto, Filipa Rego. “Avaliação da resposta ao stresse oxidativo induzido por cádmio em plantas de espinafre Spinacea oleracea L.” 2011. Thesis, Technical University of Lisbon. Accessed April 11, 2021. http://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/4182.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Pinto, Filipa Rego. “Avaliação da resposta ao stresse oxidativo induzido por cádmio em plantas de espinafre Spinacea oleracea L.” 2011. Web. 11 Apr 2021.

Vancouver:

Pinto FR. Avaliação da resposta ao stresse oxidativo induzido por cádmio em plantas de espinafre Spinacea oleracea L. [Internet] [Thesis]. Technical University of Lisbon; 2011. [cited 2021 Apr 11]. Available from: http://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/4182.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Pinto FR. Avaliação da resposta ao stresse oxidativo induzido por cádmio em plantas de espinafre Spinacea oleracea L. [Thesis]. Technical University of Lisbon; 2011. Available from: http://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/4182

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Technical University of Lisbon

7. Marques, Mónica Alexandra Madeira. A influência das práticas culturais nos níveis de nitratos em vegetais.

Degree: 2012, Technical University of Lisbon

URL: http://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/5017

►

Dissertação de Mestrado em Segurança Alimentar

Alguns vegetais, nomeadamente espinafres e alfaces, apresentam uma elevada capacidade em acumular nitratos, e é conhecido que o excesso… (more)

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Dissertação de Mestrado em Segurança Alimentar

Alguns vegetais, nomeadamente espinafres e alfaces, apresentam uma elevada capacidade em acumular nitratos, e é conhecido que o excesso de consumo deste ião pode ter implicações na saúde humana. Neste contexto, pretendeu-se determinar alguns factores que possam influenciar o teor de nitratos em alfaces e espinafres, para que seja possível, no futuro próximo, ter algum controlo sobre esse teor. Inicialmente realizou-se um ensaio de armazém com espinafre, para determinar se o tamanho do caule dos espinafres teria influência no teor de nitratos. Numa segunda fase, foram realizados três ensaios de campo de espinafre e dois ensaios de campo de alface. Pretendeu-se avaliar a influência de culturas em estufa versus ar livre, adubo de libertação controlada versus adubo convencional, quantidade de adubo colocada, hora da colheita e espinafre inteiro versus folha de espinafre. Dos resultados obtidos pode concluir-se que nenhuma das amostras de vegetais recolhidas atingiu os limites máximos estabelecidos na legislação. Constatou-se que as folhas de espinafre apresentam menor teor de nitratos que os espinafres inteiros. Concluiu-se também que a cultura de espinafre em estufa apresenta teores de nitratos superiores comparativamente à cultura de espinafre em ar livre. Propõe-se o aprofundar do conhecimento dos efeitos de práticas culturais, bem como do metabolismo dos nitratos e seus efeitos no homem, de modo a garantir melhor obtenção de rendimento das culturas sem pôr em risco a segurança alimentar dos consumidores.

ABSTRACT - Some vegetables like lettuce and spinach have a high capacity to accumulate nitrates, and we know that the excess of consumption of this ion may have implications for human health. It was intended to determine some factors that might influence the nitrate content in lettuce and spinach. Initially was performed a trial in storage with spinach to determine if the size of the spinach stalk would influence the nitrate content. In the second phase, there were three field experiments of spinach and two field experiments of lettuce. It was intended to evaluate the influence of: greenhouse crops versus outdoors, release controlled fertilizer versus conventional fertilizer, amount of fertilizer placed, time of harvest and spinach versus leaf spinach. From the results it can be concluded that none of the samples collected reached the maximum limits established by law. It was found that spinach leaves have lower nitrate content than spinach integers. It was further concluded that the spinach culture present in the greenhouse show higher levels of nitrates than the culture carried out in open air. In order to understand the effects of cultural practices as well as the metabolism of nitrates and its effects on man, further studies must be made to ensure the best crops without jeopardizing food safety.

Advisors/Committee Members: Ferreira, Marília Catarina Leal Fazeres.

Subjects/Keywords: Nitratos; alface; espinafre; Nitrates; lettuce; spinach

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APA (6^th Edition):

Marques, M. A. M. (2012). A influência das práticas culturais nos níveis de nitratos em vegetais. (Thesis). Technical University of Lisbon. Retrieved from http://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/5017

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Marques, Mónica Alexandra Madeira. “A influência das práticas culturais nos níveis de nitratos em vegetais.” 2012. Thesis, Technical University of Lisbon. Accessed April 11, 2021. http://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/5017.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Marques, Mónica Alexandra Madeira. “A influência das práticas culturais nos níveis de nitratos em vegetais.” 2012. Web. 11 Apr 2021.

Vancouver:

Marques MAM. A influência das práticas culturais nos níveis de nitratos em vegetais. [Internet] [Thesis]. Technical University of Lisbon; 2012. [cited 2021 Apr 11]. Available from: http://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/5017.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Marques MAM. A influência das práticas culturais nos níveis de nitratos em vegetais. [Thesis]. Technical University of Lisbon; 2012. Available from: http://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/5017

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Iowa State University

8. Chen, Keting. Antioxidants and dehydrin metabolism associated with osmopriming-enhanced stress tolerance of germinating spinach (Spinacia oleracea L. cv. Bloomsdale) seeds.

Degree: 2011, Iowa State University

URL: https://lib.dr.iastate.edu/etd/10471

► Osmopriming is a pre-soaking treatment that improves seed germination performance as well as stress tolerance. In this study, we use spinach (Spinacia oleracea cv. Bloomsdale)… (more)

▼ Osmopriming is a pre-soaking treatment that improves seed germination performance as well as stress tolerance. In this study, we use spinach (Spinacia oleracea cv. Bloomsdale) as the model to study cellular mechanisms contributing to the improved stress tolerance during post-priming germination. We first determined an optimal osmopriming protocol for `Bloomsdale' spinach, i.e. priming seeds with -0.6 MPa PEG 8000 at 15 yC for 8 d. This protocol also improved germinating seeds' tolerance to temperature (sub- and supra-temperature) and desiccation stress. To explore the biochemistry contributing to the priming-induced stress tolerance, we examined two extensively studied stress-responsive components: antioxidant system and dehydrins. An update of `antioxidant system' was found during osmopriming and early germination stage, as manifested in the repression of antioxidants involved in seed protection during dry storage, and enhancement of those related to seed germinability. Possibly, this system update was resulted from the transition of seeds from dry to imbibing / germinating state. Osmopriming might provide a `head-start' for this transition, and thus resulted in a more robust antioxidant system in primed seeds and increased their germination potential. Consequently, primed seeds exhibited improve tolerance to chilling and desiccation stress. Our study of dehydrin accumulation, on the other hand, suggests an alternative strategy for osmopriming to improve stress tolerance in germinating seeds. Several dehydrins (30, 26, and 19-kD dehydrins, and CAP85) transiently accumulated during osmopriming at both protein and transcript levels. These dehydrins also re-accumulated in primed seeds in response to chilling and desiccation stress, and thus may be associated with the improved stress tolerance. It is possible that osmopriming imposed mild osmotic stress in seeds and induced accumulation of stress responses (dehydrins) to confer cross-tolerance that rendered primed seeds more tolerant to subsequent stress exposures. We assumed that osmopriming might use the above-described two strategies (i.e. increasing seed germination potential and inducing cross-tolerance in seeds) that act together to enhance seed stress tolerance during post-priming germination.

Subjects/Keywords: antioxidant; dehydrin; germination; osmopriming; PEG; spinach; Horticulture

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APA (6^th Edition):

Chen, K. (2011). Antioxidants and dehydrin metabolism associated with osmopriming-enhanced stress tolerance of germinating spinach (Spinacia oleracea L. cv. Bloomsdale) seeds. (Thesis). Iowa State University. Retrieved from https://lib.dr.iastate.edu/etd/10471

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Chen, Keting. “Antioxidants and dehydrin metabolism associated with osmopriming-enhanced stress tolerance of germinating spinach (Spinacia oleracea L. cv. Bloomsdale) seeds.” 2011. Thesis, Iowa State University. Accessed April 11, 2021. https://lib.dr.iastate.edu/etd/10471.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Chen, Keting. “Antioxidants and dehydrin metabolism associated with osmopriming-enhanced stress tolerance of germinating spinach (Spinacia oleracea L. cv. Bloomsdale) seeds.” 2011. Web. 11 Apr 2021.

Vancouver:

Chen K. Antioxidants and dehydrin metabolism associated with osmopriming-enhanced stress tolerance of germinating spinach (Spinacia oleracea L. cv. Bloomsdale) seeds. [Internet] [Thesis]. Iowa State University; 2011. [cited 2021 Apr 11]. Available from: https://lib.dr.iastate.edu/etd/10471.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Chen K. Antioxidants and dehydrin metabolism associated with osmopriming-enhanced stress tolerance of germinating spinach (Spinacia oleracea L. cv. Bloomsdale) seeds. [Thesis]. Iowa State University; 2011. Available from: https://lib.dr.iastate.edu/etd/10471

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Michigan State University

9. Thoma, Sharon Leah. Molecular and cellular characterization of non-specific lipid transfer proteins from spinach and Arabidopsis.

Degree: PhD, Department of Botany and Plant Pathology, 1992, Michigan State University

URL: http://etd.lib.msu.edu/islandora/object/etd:22576

Subjects/Keywords: Lipoproteins; Spinach; Arabidopsis

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APA (6^th Edition):

Thoma, S. L. (1992). Molecular and cellular characterization of non-specific lipid transfer proteins from spinach and Arabidopsis. (Doctoral Dissertation). Michigan State University. Retrieved from http://etd.lib.msu.edu/islandora/object/etd:22576

Chicago Manual of Style (16^th Edition):

Thoma, Sharon Leah. “Molecular and cellular characterization of non-specific lipid transfer proteins from spinach and Arabidopsis.” 1992. Doctoral Dissertation, Michigan State University. Accessed April 11, 2021. http://etd.lib.msu.edu/islandora/object/etd:22576.

MLA Handbook (7^th Edition):

Thoma, Sharon Leah. “Molecular and cellular characterization of non-specific lipid transfer proteins from spinach and Arabidopsis.” 1992. Web. 11 Apr 2021.

Vancouver:

Thoma SL. Molecular and cellular characterization of non-specific lipid transfer proteins from spinach and Arabidopsis. [Internet] [Doctoral dissertation]. Michigan State University; 1992. [cited 2021 Apr 11]. Available from: http://etd.lib.msu.edu/islandora/object/etd:22576.

Council of Science Editors:

Thoma SL. Molecular and cellular characterization of non-specific lipid transfer proteins from spinach and Arabidopsis. [Doctoral Dissertation]. Michigan State University; 1992. Available from: http://etd.lib.msu.edu/islandora/object/etd:22576

Texas Tech University

10. Nalbantoglu, Barbaros. Cloning of higher plant glutamate synthase genes.

Degree: 1993, Texas Tech University

URL: http://hdl.handle.net/2346/13980

► In all plants, glutamate synthase (GOGAT) is a key enzyme involved in the assimilation of ammonia. GOGAT catalyzes the transamidation of the amido nitrogen from… (more)

Subjects/Keywords: Glutamine synthetase; Spinach

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APA (6^th Edition):

Nalbantoglu, B. (1993). Cloning of higher plant glutamate synthase genes. (Thesis). Texas Tech University. Retrieved from http://hdl.handle.net/2346/13980

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Nalbantoglu, Barbaros. “Cloning of higher plant glutamate synthase genes.” 1993. Thesis, Texas Tech University. Accessed April 11, 2021. http://hdl.handle.net/2346/13980.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Nalbantoglu, Barbaros. “Cloning of higher plant glutamate synthase genes.” 1993. Web. 11 Apr 2021.

Vancouver:

Nalbantoglu B. Cloning of higher plant glutamate synthase genes. [Internet] [Thesis]. Texas Tech University; 1993. [cited 2021 Apr 11]. Available from: http://hdl.handle.net/2346/13980.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Nalbantoglu B. Cloning of higher plant glutamate synthase genes. [Thesis]. Texas Tech University; 1993. Available from: http://hdl.handle.net/2346/13980

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Hong Kong

11. Ho, Wun-ching, Bess. Cultivation and white rust disease (Albugo sp.) of Ipomoea aquatica Forsk. in Hong Kong.

Degree: 1968, University of Hong Kong

URL: http://hdl.handle.net/10722/37991

Subjects/Keywords: Rust diseases.; Spinach.

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APA (6^th Edition):

Ho, Wun-ching, B. (1968). Cultivation and white rust disease (Albugo sp.) of Ipomoea aquatica Forsk. in Hong Kong. (Thesis). University of Hong Kong. Retrieved from http://hdl.handle.net/10722/37991

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ho, Wun-ching, Bess. “Cultivation and white rust disease (Albugo sp.) of Ipomoea aquatica Forsk. in Hong Kong.” 1968. Thesis, University of Hong Kong. Accessed April 11, 2021. http://hdl.handle.net/10722/37991.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ho, Wun-ching, Bess. “Cultivation and white rust disease (Albugo sp.) of Ipomoea aquatica Forsk. in Hong Kong.” 1968. Web. 11 Apr 2021.

Vancouver:

Ho, Wun-ching B. Cultivation and white rust disease (Albugo sp.) of Ipomoea aquatica Forsk. in Hong Kong. [Internet] [Thesis]. University of Hong Kong; 1968. [cited 2021 Apr 11]. Available from: http://hdl.handle.net/10722/37991.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ho, Wun-ching B. Cultivation and white rust disease (Albugo sp.) of Ipomoea aquatica Forsk. in Hong Kong. [Thesis]. University of Hong Kong; 1968. Available from: http://hdl.handle.net/10722/37991

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Brock University

12. Howe, Richard Charles. RNA-degrading activity associated with the ribosomes of spinach leaf chloroplasts /|nR. C. Howe. – 260 St. Catharines, Ont. : [s. n.] .

Degree: Department of Biological Sciences, 1971, Brock University

URL: http://hdl.handle.net/10464/2037

Subjects/Keywords: Nucleoproteins.; Spinach.; Chromatophores.

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APA (6^th Edition):

Howe, R. C. (1971). RNA-degrading activity associated with the ribosomes of spinach leaf chloroplasts /|nR. C. Howe. – 260 St. Catharines, Ont. : [s. n.] . (Thesis). Brock University. Retrieved from http://hdl.handle.net/10464/2037

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Howe, Richard Charles. “RNA-degrading activity associated with the ribosomes of spinach leaf chloroplasts /|nR. C. Howe. – 260 St. Catharines, Ont. : [s. n.] .” 1971. Thesis, Brock University. Accessed April 11, 2021. http://hdl.handle.net/10464/2037.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Howe, Richard Charles. “RNA-degrading activity associated with the ribosomes of spinach leaf chloroplasts /|nR. C. Howe. – 260 St. Catharines, Ont. : [s. n.] .” 1971. Web. 11 Apr 2021.

Vancouver:

Howe RC. RNA-degrading activity associated with the ribosomes of spinach leaf chloroplasts /|nR. C. Howe. – 260 St. Catharines, Ont. : [s. n.] . [Internet] [Thesis]. Brock University; 1971. [cited 2021 Apr 11]. Available from: http://hdl.handle.net/10464/2037.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Howe RC. RNA-degrading activity associated with the ribosomes of spinach leaf chloroplasts /|nR. C. Howe. – 260 St. Catharines, Ont. : [s. n.] . [Thesis]. Brock University; 1971. Available from: http://hdl.handle.net/10464/2037

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

13. Barkat, Noorani. Harvesting Time Influences Health-Promoting Properties and Nanoemulsions Inhibit E. Coli O157:H7 Growth on Spinach.

Degree: MS, Horticulture, 2017, Texas A&M University

URL: http://hdl.handle.net/1969.1/161613

► Spinach (Spinacia oleracea) is a leafy vegetable, rich in health-promoting compounds such as flavonoids, carotenoids, chlorophylls, ascorbic acid, vitamins, and dietary fiber. In the first… (more)

▼ Spinach (Spinacia oleracea) is a leafy vegetable, rich in health-promoting compounds such as flavonoids, carotenoids, chlorophylls, ascorbic acid, vitamins, and dietary fiber. In the first study, we measured levels of various phytochemicals in spinach at different growing times. Spinach was harvested at 20, 30, 40, 50, and 60 days after planting. Our results demonstrated that nitrate levels increased from 20 (1909 µg g-1) to day 40 (3668 µg g-1) then decreased (974 µg g-1) at day 60. Lutein, chlorophyll a and b were higher in 60 day samples and β-carotene contents were higher at 50 days. Twelve flavonoids were identified. Total phenolics ranged from 885 to 1162 µg/g. Spinach at 30, 40, and 60 days inhibited α-amylase activity at rates similar to acarbose (inhibitor). Bile acid binding potential was maximal in all samples. This study showed that harvest time has major effect on phytochemical levels and mature spinach has highest phytochemical levels. The second study aimed to prepare stable nanoemulsions (NE) using grapefruit and lemon essential oils (EO) to evaluate their antimicrobial properties against Escherichia coli O157:H7. Encapsulation of these EO’s in NE aided inhibition of pathogen growth on spinach during refrigerated (5°C) storage. Particle sizes of grapefruit and lemon NE were 303 and 155 nm, respectively. Spinach samples were inoculated with E. coli O157:H7, and then treated with one of three different treatments (control, 200 ppm chlorine, 5% grapefruit oil NE or 3% lemon oil NE [v/v]). The efficacy of the antimicrobial treatments was determined according to E. coli O157:H7 survival at 0, 3, 6, and 9 days of storage. Chlorine and 3% lemon NE exhibited greater iii inhibitory effects against E. coli O157:H7 versus the grapefruit NE. Nonetheless, all treatments produced significant reductions in pathogen numbers on spinach compared to untreated controls. Significant reduction was observed on each storage day with chlorine treatment and 3% lemon NE with 2.4 and 1.6 log10-cycles reduction on day 0, respectively, and the highest reductions were observed on 9th day of storage with 4.3 and 5.4 log10-cycles reduction. These findings indicated the potential of these natural EO for decontamination of fresh produce. Advisors/Committee Members: Patil, Bhimanagouda S (advisor), Jayaprakasha, G K (advisor), Taylor, Matthew (committee member).

Subjects/Keywords: Spinach; Nanoemulsion

…GROWTH ON SPINACH DURING REFRIGERATED STORAGE… …38 Inoculation of spinach… …spinach sample on different harvest days (20, 30, 40, 50 and 60). Data are expressed… …16 Figure 2. Levels of nitrate in spinach sample on different harvest days (20, 30, 40… …17 Figure 3. Chlorophyll b, a, lutein and beta carotene in spinach sample on different…

12 more images…

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APA (6^th Edition):

Barkat, N. (2017). Harvesting Time Influences Health-Promoting Properties and Nanoemulsions Inhibit E. Coli O157:H7 Growth on Spinach. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/161613

Chicago Manual of Style (16^th Edition):

Barkat, Noorani. “Harvesting Time Influences Health-Promoting Properties and Nanoemulsions Inhibit E. Coli O157:H7 Growth on Spinach.” 2017. Masters Thesis, Texas A&M University. Accessed April 11, 2021. http://hdl.handle.net/1969.1/161613.

MLA Handbook (7^th Edition):

Barkat, Noorani. “Harvesting Time Influences Health-Promoting Properties and Nanoemulsions Inhibit E. Coli O157:H7 Growth on Spinach.” 2017. Web. 11 Apr 2021.

Vancouver:

Barkat N. Harvesting Time Influences Health-Promoting Properties and Nanoemulsions Inhibit E. Coli O157:H7 Growth on Spinach. [Internet] [Masters thesis]. Texas A&M University; 2017. [cited 2021 Apr 11]. Available from: http://hdl.handle.net/1969.1/161613.

Council of Science Editors:

Barkat N. Harvesting Time Influences Health-Promoting Properties and Nanoemulsions Inhibit E. Coli O157:H7 Growth on Spinach. [Masters Thesis]. Texas A&M University; 2017. Available from: http://hdl.handle.net/1969.1/161613

University of Illinois – Urbana-Champaign

14. Palma Salgado, Sindy Paola. Artificial phylloplanes: A novel tool to study parameters linked to bacterial and viral contamination of fresh produce.

Degree: PhD, Food Science & Human Nutrition, 2018, University of Illinois – Urbana-Champaign

URL: http://hdl.handle.net/2142/101809

► Every year, one-in-six Americans suffer from a food-related illness caused by bacteria, viruses, or parasites. Since 2010, fresh produce has been linked to seven foodborne… (more)

▼ Every year, one-in-six Americans suffer from a food-related illness caused by bacteria, viruses, or parasites. Since 2010, fresh produce has been linked to seven foodborne outbreaks caused by Escherichia coli species alone. Produce surface properties, such as surface hydrophobicity and surface roughness play a key role determining the attachment of bacteria and viruses to and their removal from produce. A few previous studies have investigated the effect of surface roughness and surface hydrophobicity on the attachment and removal of bacteria and viruses from food and food contact surfaces. However, since produce surface properties undergo constant changes as a function of time and environmental factors, reports have shown inconsistent results for the same produce type with regard to bacteria attachment and removal. Researchers have realized the need to construct artificial plant surfaces to retain the surface characteristics of natural plant surfaces during sanitation tests. A few attempts have reported the use of polymers, stainless steel, zinc substrates, or alumina to fabricate surrogate surfaces that resemble food or food contact surfaces, with varying degrees of success. Nevertheless, even the most successful one among the pervious surrogate surfaces can only replicate the topographical characteristics of natural fresh produce surfaces, but not the chemical properties of the plant surfaces. Furthermore, most of the previous surrogate surfaces lack reusability due to the nature of the fabrication material. In a microbial attachment or removal study, the surrogate surface will be subjected to mechanical forces because they need to be placed in a stomacher to do the emulation; thus, the surrogate surfaces made from soft material will be damaged. The overall goal of this study is to develop a new method for the fabrication of reusable and reproducible artificial phylloplanes that replicate the three-dimensional topological features of natural produce leaves, thus having surface hydrophobicity, roughness values, and epicuticular composition resembling those of two selected fresh produce varieties. To achieve the goal, three inter-related studies were performed. In the first study, the effects of physiochemical characteristics, including produce leaf surface roughness, epicuticular wax composition, and produce and bacteria surface hydrophobicity on attachment/removal of E. coli K12 to/from plant surfaces was investigated. The attachment and removal of E. coli K12 was affected by multiple factors including produce genotype, produce surface roughness, and wax composition. Rougher surfaces resulted in higher attachment of E. coli and less removal. In addition, the removal of E. coli K12 was positively correlated with alkanes, ketones, and total wax content on the leaf surfaces. In study two, a method to create polydimethylsiloxane (PDMS)-based artificial phylloplane surface to resemble the topographical, chemical, and epicuticular characteristics of ‘Outredgeous’ romaine lettuce and ‘Carmel’ spinach to a… Advisors/Committee Members: Feng, Hao (advisor), Engeseth, Nicki J. (Committee Chair), Padua, Graciela (committee member), Nguyen, Thanh H. (committee member).

Subjects/Keywords: Fresh Produce; Lettuce; Spinach; Artificial leaf; PDMS

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APA (6^th Edition):

Palma Salgado, S. P. (2018). Artificial phylloplanes: A novel tool to study parameters linked to bacterial and viral contamination of fresh produce. (Doctoral Dissertation). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/101809

Chicago Manual of Style (16^th Edition):

Palma Salgado, Sindy Paola. “Artificial phylloplanes: A novel tool to study parameters linked to bacterial and viral contamination of fresh produce.” 2018. Doctoral Dissertation, University of Illinois – Urbana-Champaign. Accessed April 11, 2021. http://hdl.handle.net/2142/101809.

MLA Handbook (7^th Edition):

Palma Salgado, Sindy Paola. “Artificial phylloplanes: A novel tool to study parameters linked to bacterial and viral contamination of fresh produce.” 2018. Web. 11 Apr 2021.

Vancouver:

Palma Salgado SP. Artificial phylloplanes: A novel tool to study parameters linked to bacterial and viral contamination of fresh produce. [Internet] [Doctoral dissertation]. University of Illinois – Urbana-Champaign; 2018. [cited 2021 Apr 11]. Available from: http://hdl.handle.net/2142/101809.

Council of Science Editors:

Palma Salgado SP. Artificial phylloplanes: A novel tool to study parameters linked to bacterial and viral contamination of fresh produce. [Doctoral Dissertation]. University of Illinois – Urbana-Champaign; 2018. Available from: http://hdl.handle.net/2142/101809

15. Defelice, Domenic. Effect of freezing, canning and drying on the vitamin A (Carotene) content of spinach.

Degree: Food Technology, 1937, University of Massachusetts

URL: https://scholarworks.umass.edu/theses/1448

Subjects/Keywords: Spinach; Vitamin A

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APA (6^th Edition):

Defelice, D. (1937). Effect of freezing, canning and drying on the vitamin A (Carotene) content of spinach. (Thesis). University of Massachusetts. Retrieved from https://scholarworks.umass.edu/theses/1448

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Defelice, Domenic. “Effect of freezing, canning and drying on the vitamin A (Carotene) content of spinach.” 1937. Thesis, University of Massachusetts. Accessed April 11, 2021. https://scholarworks.umass.edu/theses/1448.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Defelice, Domenic. “Effect of freezing, canning and drying on the vitamin A (Carotene) content of spinach.” 1937. Web. 11 Apr 2021.

Vancouver:

Defelice D. Effect of freezing, canning and drying on the vitamin A (Carotene) content of spinach. [Internet] [Thesis]. University of Massachusetts; 1937. [cited 2021 Apr 11]. Available from: https://scholarworks.umass.edu/theses/1448.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Defelice D. Effect of freezing, canning and drying on the vitamin A (Carotene) content of spinach. [Thesis]. University of Massachusetts; 1937. Available from: https://scholarworks.umass.edu/theses/1448

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Université Paris-Sud – Paris XI

16. Okuda, Maho. Mécanisme d’action d’une classe d’antibiotiques depuis leur entrée jusqu’à leur cible chez la bactérie : visualisation en temps réel : Mechanism of action of a class of antibiotics from their entry to their target in bacteria : a real time visualization.

Degree: Docteur es, Sciences de la vie et de la santé, 2015, Université Paris-Sud – Paris XI

URL: http://www.theses.fr/2015PA112239

►

Des techniques variées de visualisation de molécules d’intérêt sur cellules vivantes ou fixées ont permis de suivre leur synthèse, localisation, dégradation et autres activités. Dans… (more)

▼

Des techniques variées de visualisation de molécules d’intérêt sur cellules vivantes ou fixées ont permis de suivre leur synthèse, localisation, dégradation et autres activités. Dans cette étude, nous avons développé deux outils de fluorescence pour étudier la synthèse des protéines sur bactéries vivantes. Le premier décrit l’utilisation du système Spinach pour l’imagerie du ribosome. Cette approche diffère des méthodes conventionnelles qui utilisent des protéines fluorescentes puisque l’ARN ribosomal 16S contient un aptamère qui rend fluorescent un composé fluorogène. Une étude comparative de la performance de différents aptamères Spinach a été réalisée. Un deuxième outil se focalise sur l’accumulation d’un antibiotique de la famille des aminoglycosides (ligand du ribosome) conjugué à un fluorophore. Ce nouveau conjugué, qui a conservé son activité bactéricide permet pour la première fois de visualiser l’accumulation de l’antibiotique sur bactérie vivante. Cela permet une analyse au niveau de la cellule unique d’une population bactérienne exposée à l’antibiotique. Nous avons également obtenu des données sur la localisation de l’antibiotique une fois qu’il a pénétré dans la bactérie à une résolution inégalée par microscopie super-résolutive. Nous espérons que ces deux méthodes vont maintenant permettre une meilleure compréhension de la synthèse des protéines et fournir une vue nouvelle de la pénétration des antibiotiques dans les bactéries pour y produire leur action bactéricide.

Various visualizing techniques have previously enabled monitoring the fate of molecules of interest: their expression, localization, degradation and other activities in live or fixed cells. In this study, we have developed two fluorescent tools to study protein synthesis in live bacterial cell. The first one describes the application of Spinach system to ribosomes imaging. This is different from conventional methods (that use fluorescent proteins) in that 16S rRNA contains an inserted RNA aptamer that elicits fluorescence of a fluorogenic compound. A comparative study of the performance of different Spinach aptamers was performed here. A second system focuses on the uptake of a fluorescently labeled ligand of the ribosome, an antibiotic of the class of aminoglycosides. This novel conjugate, which kept its bactericidal activity allows for the first time imaging of aminoglycoside uptake on live bacteria. This opened the door to a single cell analysis of bacterial cell populations. We also obtained data about the localization of the antibiotic once inside the bacteria to an unprecedented resolution using super resolution microscopy. We hope that both of these methods will contribute to a better understanding of protein synthesis as well as provide a novel view on the way antibiotics penetrate into cells and perform their bactericidal action.

Advisors/Committee Members: Yoshizawa, Satoko (thesis director), Fourmy, Dominique (thesis director).

Subjects/Keywords: Microscopie de fluorescence; Microscopie super-résolutive; Traduction; Ribosome; Aminoglycosides; Aptamère Spinach; Fluorescence microscopy; Super-resolution microscopy; Translation; Ribosome; Aminoglycosides; Spinach aptamer

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APA (6^th Edition):

Okuda, M. (2015). Mécanisme d’action d’une classe d’antibiotiques depuis leur entrée jusqu’à leur cible chez la bactérie : visualisation en temps réel : Mechanism of action of a class of antibiotics from their entry to their target in bacteria : a real time visualization. (Doctoral Dissertation). Université Paris-Sud – Paris XI. Retrieved from http://www.theses.fr/2015PA112239

Chicago Manual of Style (16^th Edition):

Okuda, Maho. “Mécanisme d’action d’une classe d’antibiotiques depuis leur entrée jusqu’à leur cible chez la bactérie : visualisation en temps réel : Mechanism of action of a class of antibiotics from their entry to their target in bacteria : a real time visualization.” 2015. Doctoral Dissertation, Université Paris-Sud – Paris XI. Accessed April 11, 2021. http://www.theses.fr/2015PA112239.

MLA Handbook (7^th Edition):

Okuda, Maho. “Mécanisme d’action d’une classe d’antibiotiques depuis leur entrée jusqu’à leur cible chez la bactérie : visualisation en temps réel : Mechanism of action of a class of antibiotics from their entry to their target in bacteria : a real time visualization.” 2015. Web. 11 Apr 2021.

Vancouver:

Okuda M. Mécanisme d’action d’une classe d’antibiotiques depuis leur entrée jusqu’à leur cible chez la bactérie : visualisation en temps réel : Mechanism of action of a class of antibiotics from their entry to their target in bacteria : a real time visualization. [Internet] [Doctoral dissertation]. Université Paris-Sud – Paris XI; 2015. [cited 2021 Apr 11]. Available from: http://www.theses.fr/2015PA112239.

Council of Science Editors:

Okuda M. Mécanisme d’action d’une classe d’antibiotiques depuis leur entrée jusqu’à leur cible chez la bactérie : visualisation en temps réel : Mechanism of action of a class of antibiotics from their entry to their target in bacteria : a real time visualization. [Doctoral Dissertation]. Université Paris-Sud – Paris XI; 2015. Available from: http://www.theses.fr/2015PA112239

Michigan State University

17. Metzger, James David. Gibberellins and the photoperiodic control of stem growth in the long-day rosette plant spinach.

Degree: PhD, Department of Botany and Plant Pathology, 1980, Michigan State University

URL: http://etd.lib.msu.edu/islandora/object/etd:21942

Subjects/Keywords: Spinach; Spinach – Growth; Plant photoperiodism; Gibberellins; Stems (Botany)

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APA (6^th Edition):

Metzger, J. D. (1980). Gibberellins and the photoperiodic control of stem growth in the long-day rosette plant spinach. (Doctoral Dissertation). Michigan State University. Retrieved from http://etd.lib.msu.edu/islandora/object/etd:21942

Chicago Manual of Style (16^th Edition):

Metzger, James David. “Gibberellins and the photoperiodic control of stem growth in the long-day rosette plant spinach.” 1980. Doctoral Dissertation, Michigan State University. Accessed April 11, 2021. http://etd.lib.msu.edu/islandora/object/etd:21942.

MLA Handbook (7^th Edition):

Metzger, James David. “Gibberellins and the photoperiodic control of stem growth in the long-day rosette plant spinach.” 1980. Web. 11 Apr 2021.

Vancouver:

Metzger JD. Gibberellins and the photoperiodic control of stem growth in the long-day rosette plant spinach. [Internet] [Doctoral dissertation]. Michigan State University; 1980. [cited 2021 Apr 11]. Available from: http://etd.lib.msu.edu/islandora/object/etd:21942.

Council of Science Editors:

Metzger JD. Gibberellins and the photoperiodic control of stem growth in the long-day rosette plant spinach. [Doctoral Dissertation]. Michigan State University; 1980. Available from: http://etd.lib.msu.edu/islandora/object/etd:21942

McGill University

18. Chimphango, Annie F. A. Diffusion channel system for controlled atmosphere storage of spinach.

Degree: MS, Department of Agricultural and Biosystems Engineering, 1996, McGill University

URL: https://escholarship.mcgill.ca/downloads/1v53k0050.pdf ; https://escholarship.mcgill.ca/concern/theses/v979v4954

► Advanced research in Controlled/Modified Atmosphere storage systems has shown that open channels of different lengths and cross sectional areas, connected to an air tight storage… (more)

Subjects/Keywords: Spinach – Postharvest technology.; Protective atmospheres.; Spinach – Storage.

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APA (6^th Edition):

Chimphango, A. F. A. (1996). Diffusion channel system for controlled atmosphere storage of spinach. (Masters Thesis). McGill University. Retrieved from https://escholarship.mcgill.ca/downloads/1v53k0050.pdf ; https://escholarship.mcgill.ca/concern/theses/v979v4954

Chicago Manual of Style (16^th Edition):

Chimphango, Annie F A. “Diffusion channel system for controlled atmosphere storage of spinach.” 1996. Masters Thesis, McGill University. Accessed April 11, 2021. https://escholarship.mcgill.ca/downloads/1v53k0050.pdf ; https://escholarship.mcgill.ca/concern/theses/v979v4954.

MLA Handbook (7^th Edition):

Chimphango, Annie F A. “Diffusion channel system for controlled atmosphere storage of spinach.” 1996. Web. 11 Apr 2021.

Vancouver:

Chimphango AFA. Diffusion channel system for controlled atmosphere storage of spinach. [Internet] [Masters thesis]. McGill University; 1996. [cited 2021 Apr 11]. Available from: https://escholarship.mcgill.ca/downloads/1v53k0050.pdf ; https://escholarship.mcgill.ca/concern/theses/v979v4954.

Council of Science Editors:

Chimphango AFA. Diffusion channel system for controlled atmosphere storage of spinach. [Masters Thesis]. McGill University; 1996. Available from: https://escholarship.mcgill.ca/downloads/1v53k0050.pdf ; https://escholarship.mcgill.ca/concern/theses/v979v4954

University of Georgia

19. Doering, Helga J. A systems approach to determine the fate of Escherichia coli O157:H7 on fresh and fresh-cut iceberg lettuce and spinach.

Degree: 2014, University of Georgia

URL: http://hdl.handle.net/10724/25433

► The systems approach was used to determine the fate of Escherichia coli O157:H7 on iceberg lettuce and spinach under conditions that mimic those from field… (more)

Subjects/Keywords: Spinach; lettuce; Escherichia coli O157:H7; systems approach

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APA (6^th Edition):

Doering, H. J. (2014). A systems approach to determine the fate of Escherichia coli O157:H7 on fresh and fresh-cut iceberg lettuce and spinach. (Thesis). University of Georgia. Retrieved from http://hdl.handle.net/10724/25433

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Doering, Helga J. “A systems approach to determine the fate of Escherichia coli O157:H7 on fresh and fresh-cut iceberg lettuce and spinach.” 2014. Thesis, University of Georgia. Accessed April 11, 2021. http://hdl.handle.net/10724/25433.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Doering, Helga J. “A systems approach to determine the fate of Escherichia coli O157:H7 on fresh and fresh-cut iceberg lettuce and spinach.” 2014. Web. 11 Apr 2021.

Vancouver:

Doering HJ. A systems approach to determine the fate of Escherichia coli O157:H7 on fresh and fresh-cut iceberg lettuce and spinach. [Internet] [Thesis]. University of Georgia; 2014. [cited 2021 Apr 11]. Available from: http://hdl.handle.net/10724/25433.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Doering HJ. A systems approach to determine the fate of Escherichia coli O157:H7 on fresh and fresh-cut iceberg lettuce and spinach. [Thesis]. University of Georgia; 2014. Available from: http://hdl.handle.net/10724/25433

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Texas A&M University

20. Calix Lara, Thelma. Quantification of the Antimicrobial Substances Produced by Lactic Acid Bacteria used as an Intervention to Inhibit Escherichia coli O157:H7 and Salmonella in vitro and on Fresh Spinach (Spinacia oleracea).

Degree: PhD, Food Science and Technology, 2012, Texas A&M University

URL: http://hdl.handle.net/1969.1/ETD-TAMU-2011-12-10296

► The metabolic activity of bacterial microorganisms may influence the growth and metabolic activities of other microbes that are present in any specific niche. Lactic acid… (more)

▼ The metabolic activity of bacterial microorganisms may influence the growth and metabolic activities of other microbes that are present in any specific niche. Lactic acid bacteria (LAB) are antagonistic to some microbial pathogens by the metabolic production of compounds with antimicrobial activity. Consequently, investigators have measured the effects of those antimicrobials to inhibit specific pathogens. However, the mode(s) of action of LAB against foodborne pathogens on products and/or in broth is not completely understood. Therefore, the objectives of this research were to (i) determine the LAB dose required for inhibition of Escherichia coli O157:H7 and Salmonella enterica in vitro and on spinach, and (ii) identify and quantify the major antimicrobial substances synthesized by LAB as a function of postinoculation storage conditions. Assays were performed at 7 degrees C under aerobic conditions. The foodborne pathogens dose responses were assessed in a liquid microbiological medium (in vitro) and on spinach leaf surfaces. Different levels of foodborne pathogens and LAB cultures were used. The addition of LAB cultures did not reduce E. coli O157:H7 or Salmonella enterica populations when performed in vitro. However, when LAB cultures were sprayed on the surfaces of spinach leaves at 8.0 log10 CFU/g, there were significant reductions on E. coli O157:H7 of 1.62 and 0.73 log10 CFU/g (after 3 days) and on Salmonella enterica of 1.85 and 0.71 log10 CFU/g (after 6 days) for treatments inoculated with an initial level of 2.0 and 4.0 log10 CFU/g, respectively. After quantification of the antimicrobial compounds synthesized by LAB cultures, they were correlated against the population growth of targeted pathogens. The highest Llactic acid (3.71 plus/minus 0.14 micromoles/ml, day 12) and hydrogen peroxide (3.72 plus/minus 3.34 microM, day 6) production were obtained from the in vitro sample inoculated with 8.0 log10 CFU/ml of LAB and 0.0 log10 CFU/ml of pathogens. The highest bacteriocin production (0.1 plus/minus 0.01 mg/ml) was obtained from the in vitro sample with 8.0 log10 CFU/ml of LAB and 2.0 log10 CFU/ml of pathogens. In conclusion, the LAB cultures were able to produce detectable amounts of antimicrobials that may be used as intervention and/or sciencebased practice against foodborne pathogens by producers and the industry. Advisors/Committee Members: Taylor, Thomas M. (advisor), Castillo Ayala, Alejandro, Smith, Stephen B. (committee member), Miller, Rhonda, Sturino, Joseph (committee member).

Subjects/Keywords: E. coli O157:H7; Salmonella; Lactic acid bacteria; bacteriocin; spinach

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APA (6^th Edition):

Calix Lara, T. (2012). Quantification of the Antimicrobial Substances Produced by Lactic Acid Bacteria used as an Intervention to Inhibit Escherichia coli O157:H7 and Salmonella in vitro and on Fresh Spinach (Spinacia oleracea). (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-2011-12-10296

Chicago Manual of Style (16^th Edition):

Calix Lara, Thelma. “Quantification of the Antimicrobial Substances Produced by Lactic Acid Bacteria used as an Intervention to Inhibit Escherichia coli O157:H7 and Salmonella in vitro and on Fresh Spinach (Spinacia oleracea).” 2012. Doctoral Dissertation, Texas A&M University. Accessed April 11, 2021. http://hdl.handle.net/1969.1/ETD-TAMU-2011-12-10296.

MLA Handbook (7^th Edition):

Calix Lara, Thelma. “Quantification of the Antimicrobial Substances Produced by Lactic Acid Bacteria used as an Intervention to Inhibit Escherichia coli O157:H7 and Salmonella in vitro and on Fresh Spinach (Spinacia oleracea).” 2012. Web. 11 Apr 2021.

Vancouver:

Calix Lara T. Quantification of the Antimicrobial Substances Produced by Lactic Acid Bacteria used as an Intervention to Inhibit Escherichia coli O157:H7 and Salmonella in vitro and on Fresh Spinach (Spinacia oleracea). [Internet] [Doctoral dissertation]. Texas A&M University; 2012. [cited 2021 Apr 11]. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2011-12-10296.

Council of Science Editors:

Calix Lara T. Quantification of the Antimicrobial Substances Produced by Lactic Acid Bacteria used as an Intervention to Inhibit Escherichia coli O157:H7 and Salmonella in vitro and on Fresh Spinach (Spinacia oleracea). [Doctoral Dissertation]. Texas A&M University; 2012. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2011-12-10296

University of the Western Cape

21. Martin, Annamarie Guinnevere. Vegetable farms in Cape Town: water quality and possible remediation techniques .

Degree: 2012, University of the Western Cape

URL: http://hdl.handle.net/11394/4398

► Heavy metal contamination tends to be a problem in inner city agricultural areas and gardens. High levels of certain heavy metals have been found in… (more)

Subjects/Keywords: Cadmium; Lead; Turnip; Spinach; Irrigation water; Heavy metals

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APA (6^th Edition):

Martin, A. G. (2012). Vegetable farms in Cape Town: water quality and possible remediation techniques . (Thesis). University of the Western Cape. Retrieved from http://hdl.handle.net/11394/4398

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Martin, Annamarie Guinnevere. “Vegetable farms in Cape Town: water quality and possible remediation techniques .” 2012. Thesis, University of the Western Cape. Accessed April 11, 2021. http://hdl.handle.net/11394/4398.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Martin, Annamarie Guinnevere. “Vegetable farms in Cape Town: water quality and possible remediation techniques .” 2012. Web. 11 Apr 2021.

Vancouver:

Martin AG. Vegetable farms in Cape Town: water quality and possible remediation techniques . [Internet] [Thesis]. University of the Western Cape; 2012. [cited 2021 Apr 11]. Available from: http://hdl.handle.net/11394/4398.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Martin AG. Vegetable farms in Cape Town: water quality and possible remediation techniques . [Thesis]. University of the Western Cape; 2012. Available from: http://hdl.handle.net/11394/4398

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Michigan State University

22. Bemis, William Putnam. A cytogenetic investigation of sex expression in Spinacia.

Degree: PhD, 1952, Michigan State University

URL: http://etd.lib.msu.edu/islandora/object/etd:5165

Subjects/Keywords: Spinach; Plants, Sex in; Genetics

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APA (6^th Edition):

Bemis, W. P. (1952). A cytogenetic investigation of sex expression in Spinacia. (Doctoral Dissertation). Michigan State University. Retrieved from http://etd.lib.msu.edu/islandora/object/etd:5165

Chicago Manual of Style (16^th Edition):

Bemis, William Putnam. “A cytogenetic investigation of sex expression in Spinacia.” 1952. Doctoral Dissertation, Michigan State University. Accessed April 11, 2021. http://etd.lib.msu.edu/islandora/object/etd:5165.

MLA Handbook (7^th Edition):

Bemis, William Putnam. “A cytogenetic investigation of sex expression in Spinacia.” 1952. Web. 11 Apr 2021.

Vancouver:

Bemis WP. A cytogenetic investigation of sex expression in Spinacia. [Internet] [Doctoral dissertation]. Michigan State University; 1952. [cited 2021 Apr 11]. Available from: http://etd.lib.msu.edu/islandora/object/etd:5165.

Council of Science Editors:

Bemis WP. A cytogenetic investigation of sex expression in Spinacia. [Doctoral Dissertation]. Michigan State University; 1952. Available from: http://etd.lib.msu.edu/islandora/object/etd:5165

Southern Illinois University

23. Vigardt, April Lorraine. Influence of coffee vermicompost on growth and nutrient quality of greenhouse spinach and field grown green bell peppers.

Degree: MS, Plant and Soil Science, 2012, Southern Illinois University

URL: https://opensiuc.lib.siu.edu/theses/918

► The vermicomposting of coffee grounds shows great promise for urban areas and university campuses. Several studies have examined using coffee grounds as a substrate… (more)

▼ The vermicomposting of coffee grounds shows great promise for urban areas and university campuses. Several studies have examined using coffee grounds as a substrate for vermicomposting, however, little is known about its effect on plant growth, yield and quality. Therefore, two studies were conducted to assess these effects on greenhouse spinach and field grown bell peppers. Coffee vermicompost (VC) was utilized in a greenhouse spinach study over two spring growing seasons (2011 and 2012). Coffee VC was added to a 1:1:1 (peat, soil, sand) medium by volume (0, 25, 50 and 75%) in 4.5 L clay pots and seeded with `Bloomsdale Longstanding' spinach (Spinacia oleracea). Growth parameters evaluated were fresh leaf weight (FLW), leaf area (LA), spinach plant height, number of leaves, fresh leaf ascorbic acid (AA) and dry leaf nitrate (DLN). In 2011, the highest FLW, LA, number of leaves, AA and nitrate content were seen at the 75% VC application rate. Many parameters were correlated: The FLW and the VC application rate (r=0.41, P<0.0001); the AA content in leaves and the VC application rate (r=0.60, P<0.0001); and the AA and nitrate content in leaves (r=0.45, P=0.011). In 2012, the greatest FLW, height and number of leaves were observed at the 50% VC application rate; the highest nitrate content at 75% VC application rate, with no difference in AA content. A field study was conducted over three seasons (2009–-2011) to compare four treatments (coffee VC, dairy compost, standard fertility (SFT) and no treatment) for their effects on growth, yield, and AA content of bell peppers. Coffee VC and dairy compost were applied to beds at the rate of 22 t/ha and SFT was applied as 212 kg/ha 12:12:12 (N:P:K). Parameters evaluated were total number and weight of marketable and cull (unmarketable) pepper fruits, plant height, leaf chlorophyll index, and fresh fruit AA content. There were no differences detected for pepper fruit yields or AA content, however, plant height and chlorophyll index were greater for the VC and SFT treatments than for the compost and control treatments. These results indicated that coffee VC can improve the yield of greenhouse spinach and that the AA content does not decrease with higher VC application rates, even as nitrate content increases. Results of the bell pepper field study indicated that the coffee VC treatment produces similar growth, yield and AA content as SFT. Advisors/Committee Members: Walters, Stuart.

Subjects/Keywords: ascorbic acid; bell peppers; nitrate; nitrogen assimilation; spinach; vermicompost

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APA (6^th Edition):

Vigardt, A. L. (2012). Influence of coffee vermicompost on growth and nutrient quality of greenhouse spinach and field grown green bell peppers. (Masters Thesis). Southern Illinois University. Retrieved from https://opensiuc.lib.siu.edu/theses/918

Chicago Manual of Style (16^th Edition):

Vigardt, April Lorraine. “Influence of coffee vermicompost on growth and nutrient quality of greenhouse spinach and field grown green bell peppers.” 2012. Masters Thesis, Southern Illinois University. Accessed April 11, 2021. https://opensiuc.lib.siu.edu/theses/918.

MLA Handbook (7^th Edition):

Vigardt, April Lorraine. “Influence of coffee vermicompost on growth and nutrient quality of greenhouse spinach and field grown green bell peppers.” 2012. Web. 11 Apr 2021.

Vancouver:

Vigardt AL. Influence of coffee vermicompost on growth and nutrient quality of greenhouse spinach and field grown green bell peppers. [Internet] [Masters thesis]. Southern Illinois University; 2012. [cited 2021 Apr 11]. Available from: https://opensiuc.lib.siu.edu/theses/918.

Council of Science Editors:

Vigardt AL. Influence of coffee vermicompost on growth and nutrient quality of greenhouse spinach and field grown green bell peppers. [Masters Thesis]. Southern Illinois University; 2012. Available from: https://opensiuc.lib.siu.edu/theses/918

Brock University

24. Carpenter, Christene. Effect of pH on non-photochemical quenching in spinach photsystem 2 particles as measured by picosecond fluorescence decay kinetics .

Degree: Department of Biological Sciences, 1995, Brock University

URL: http://hdl.handle.net/10464/2783

► Single photon timing was used to study picosecond chlorophyll a fluorescence decay kinetics of pH induced non-photochemical quenching in spinach photosystem 2 particles. The characteristics… (more)

Subjects/Keywords: Photochemisty.; Chlorophyll.; Fluorescence.; Spinach.

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APA (6^th Edition):

Carpenter, C. (1995). Effect of pH on non-photochemical quenching in spinach photsystem 2 particles as measured by picosecond fluorescence decay kinetics . (Thesis). Brock University. Retrieved from http://hdl.handle.net/10464/2783

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Carpenter, Christene. “Effect of pH on non-photochemical quenching in spinach photsystem 2 particles as measured by picosecond fluorescence decay kinetics .” 1995. Thesis, Brock University. Accessed April 11, 2021. http://hdl.handle.net/10464/2783.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Carpenter, Christene. “Effect of pH on non-photochemical quenching in spinach photsystem 2 particles as measured by picosecond fluorescence decay kinetics .” 1995. Web. 11 Apr 2021.

Vancouver:

Carpenter C. Effect of pH on non-photochemical quenching in spinach photsystem 2 particles as measured by picosecond fluorescence decay kinetics . [Internet] [Thesis]. Brock University; 1995. [cited 2021 Apr 11]. Available from: http://hdl.handle.net/10464/2783.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Carpenter C. Effect of pH on non-photochemical quenching in spinach photsystem 2 particles as measured by picosecond fluorescence decay kinetics . [Thesis]. Brock University; 1995. Available from: http://hdl.handle.net/10464/2783

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Arkansas

25. Yang, Wei. Initial Fine Mapping of the Spinach Downy Mildew Resistance Locus RPF1.

Degree: MS, 2013, University of Arkansas

URL: https://scholarworks.uark.edu/etd/781

► Spinach is an important vegetable crop grown in many parts of the world (Correll et al. 2011). It has become increasingly popular due to… (more)

▼ Spinach is an important vegetable crop grown in many parts of the world (Correll et al. 2011). It has become increasingly popular due to its nutritional value as it is very high in antioxidants (Prior, 2003). Spinach production, however, is affected by many biotic stresses. Downy mildew, caused by Peronospora farinosa f. sp. spinaciae (Pfs) , is perhaps the most important biotic constraint for spinach production worldwide (Correll et al, 1994). As of 2012, 14 races of Pfs have been reported (Correll et al., 2011). The rapid increase of new races is likely a direct result of substantial changes in spinach production during the past decade, including a rapid expansion of planted acreage, 12-month per year production cycles, high density plantings, reduced usage of rotation crops, and possibly the global movement of the pathogen either as mycelium in seed or as dormant oospores (Inaba and Morinaka, 1984; Inaba et al., 1983). Very few studies have been published on the genetics of resistance to Pfs . However, several more recent studies on the genetics of resistance have been conducted and it has been hypothesized that there are at least six resistance loci (RPF1-RPF6) which control resistance to Pfs races 1-14 (Correll et al., 2007; 2011; Irish et al., 2008). Irish et al. (2008) also identified a molecular marker, designated Dm-1, which was closely linked to the resistance locus <em>RPF1. The Dm-1 marker was a co-dominant marker and the genetic distance between Dm-1 and RPF1 was estimated to be 1.7 cM. In the current study, efforts were initiated to fine-map the Dm-1 marker relative to the RPF1 locus. To address this objective, a spinach BAC library that was previously developed from line NIL1 containing Dm-1 and RPF1 was evaluated. Southern blot hybridizations identified 19 BAC clones that contained Dm-1. Dm-1 was subsequently confirmed in these 19 clones based on PCR analysis. Out of over 75,000 clones evaluated, BAC-end sequencing was conducted on 14 of the 19 BAC clones and the sequence information was used to develop probes for Southern hybridizations. Hybridizations with the 28 probes developed were conducted to identify the BAC-end furthest from the Dm-1 marker. These activities provided important sequence information about the region surrounding the Dm-1 marker, and refined a protocol to map and ultimately clone the RPF1 locus through BAC-based chromosome walking combined with genetic mapping. </em> Advisors/Committee Members: James C. Correll, Burton H. Bluhm, Pengyin Chen.

Subjects/Keywords: Biological sciences; Downy mildew; Resistance loci; Spinach; Plant Pathology

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APA (6^th Edition):

Yang, W. (2013). Initial Fine Mapping of the Spinach Downy Mildew Resistance Locus RPF1. (Masters Thesis). University of Arkansas. Retrieved from https://scholarworks.uark.edu/etd/781

Chicago Manual of Style (16^th Edition):

Yang, Wei. “Initial Fine Mapping of the Spinach Downy Mildew Resistance Locus RPF1.” 2013. Masters Thesis, University of Arkansas. Accessed April 11, 2021. https://scholarworks.uark.edu/etd/781.

MLA Handbook (7^th Edition):

Yang, Wei. “Initial Fine Mapping of the Spinach Downy Mildew Resistance Locus RPF1.” 2013. Web. 11 Apr 2021.

Vancouver:

Yang W. Initial Fine Mapping of the Spinach Downy Mildew Resistance Locus RPF1. [Internet] [Masters thesis]. University of Arkansas; 2013. [cited 2021 Apr 11]. Available from: https://scholarworks.uark.edu/etd/781.

Council of Science Editors:

Yang W. Initial Fine Mapping of the Spinach Downy Mildew Resistance Locus RPF1. [Masters Thesis]. University of Arkansas; 2013. Available from: https://scholarworks.uark.edu/etd/781

Kansas State University

26. Batziakas, Konstantinos. Reducing food losses and improving the quality of locally produced spinach.

Degree: PhD, Department of Horticulture and Natural Resources, 2019, Kansas State University

URL: http://hdl.handle.net/2097/40241

► Food loss and waste (FLW) constitute a substantial problem for global food security. Particularly, fresh produce constitutes 44% (by weight) of the global FLW. FLW… (more)

▼ Food loss and waste (FLW) constitute a substantial problem for global food security. Particularly, fresh produce constitutes 44% (by weight) of the global FLW. FLW of fresh produce occurs throughout the food chain, from production to the consumer. In the Central U.S., many small-acreage fruit and vegetable growers are utilizing high tunnels, which have been successful at increasing the yield of several crops. However, little is known about the effect of this production system on FLW. Moreover, small acreage producers have limited access to postharvest handling resources like refrigeration, which can lead to FLW during storage. Finally, consumer dissatisfaction is one of the main drivers of postharvest food waste in fresh produce. The overall objective of this dissertation was to investigate interventions for reducing FLW of locally produced spinach (Spinacia oleracea cv. Corvair). The effect of the high tunnel production system on preharvest losses, quality at harvest and during storage, consumer acceptability, and shelf life of spinach were examined and compared to the open field production system. We also evaluated the effect of passive Modified Atmosphere Packaging (MAP) on the quality and shelf life of locally grown spinach stored at non-optimum temperatures of 13 and 21 °C. Field experiments were carried out at the Kansas State University Olathe Horticulture Center from 2014 to 2017. We utilized a systems approach with six replications for each growing system. Shelf life experiments were performed at near-optimum and non-optimum temperatures. Spinach produced in high tunnels consistently demonstrated significantly higher marketable yield and higher percent marketability when compared to spinach produced in the open field. Both production systems produce spinach of premium physical and nutritional quality. There were no differences between the two treatments when spinach was stored at 3 °C. However, high tunnel spinach demonstrated improved postharvest behavior at 13 °C due to reduced respiration and yellowing rate and increased water content when compared to the open field. Consumer acceptability and sensory characteristics of spinach grown in the two systems were evaluated using a consumer study and descriptive sensory analysis. Consumers preferred spinach produced in high tunnels in terms of overall liking, flavor liking, and texture liking when compared to the open field and non-local spinach. Descriptive analysis showed that locally grown spinach had a higher intensity of attributes that indicate premium quality, such as green color and green/spinach flavors. BreatheWay® technology was investigated for the passive MAP experiments and spinach was stored at non-optimum temperatures. Spinach stored in MAP demonstrated a storage life extension, due to a slower rate of yellowing and water loss. The results of this work indicate that high tunnel production can reduce the FLW of spinach and produce a crop of high organoleptic quality that is preferred by consumers. Passive MAP has the potential to extend the… Advisors/Committee Members: Eleni Pliakoni.

Subjects/Keywords: Spinach; Local food; Food losses; Postharvest; High tunnels; Food security

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APA (6^th Edition):

Batziakas, K. (2019). Reducing food losses and improving the quality of locally produced spinach. (Doctoral Dissertation). Kansas State University. Retrieved from http://hdl.handle.net/2097/40241

Chicago Manual of Style (16^th Edition):

Batziakas, Konstantinos. “Reducing food losses and improving the quality of locally produced spinach.” 2019. Doctoral Dissertation, Kansas State University. Accessed April 11, 2021. http://hdl.handle.net/2097/40241.

MLA Handbook (7^th Edition):

Batziakas, Konstantinos. “Reducing food losses and improving the quality of locally produced spinach.” 2019. Web. 11 Apr 2021.

Vancouver:

Batziakas K. Reducing food losses and improving the quality of locally produced spinach. [Internet] [Doctoral dissertation]. Kansas State University; 2019. [cited 2021 Apr 11]. Available from: http://hdl.handle.net/2097/40241.

Council of Science Editors:

Batziakas K. Reducing food losses and improving the quality of locally produced spinach. [Doctoral Dissertation]. Kansas State University; 2019. Available from: http://hdl.handle.net/2097/40241

University of Georgia

27. McCullum, Nakieta Monque. Behavior of Escherichia coli O157:H7 on lettuce and spinach and Salmonella montevideo on tomatoes.

Degree: 2014, University of Georgia

URL: http://hdl.handle.net/10724/25562

► Escherichia coli O157:H7 and Salmonella spp. are the two organisms most commonly associated with bacterial foodborne illness attributed to the consumption of fresh produce. The… (more)

Subjects/Keywords: Salmonella; Escherichia coli O157:H7; Tomato; Lettuce; Spinach

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APA (6^th Edition):

McCullum, N. M. (2014). Behavior of Escherichia coli O157:H7 on lettuce and spinach and Salmonella montevideo on tomatoes. (Thesis). University of Georgia. Retrieved from http://hdl.handle.net/10724/25562

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

McCullum, Nakieta Monque. “Behavior of Escherichia coli O157:H7 on lettuce and spinach and Salmonella montevideo on tomatoes.” 2014. Thesis, University of Georgia. Accessed April 11, 2021. http://hdl.handle.net/10724/25562.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

McCullum, Nakieta Monque. “Behavior of Escherichia coli O157:H7 on lettuce and spinach and Salmonella montevideo on tomatoes.” 2014. Web. 11 Apr 2021.

Vancouver:

McCullum NM. Behavior of Escherichia coli O157:H7 on lettuce and spinach and Salmonella montevideo on tomatoes. [Internet] [Thesis]. University of Georgia; 2014. [cited 2021 Apr 11]. Available from: http://hdl.handle.net/10724/25562.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

McCullum NM. Behavior of Escherichia coli O157:H7 on lettuce and spinach and Salmonella montevideo on tomatoes. [Thesis]. University of Georgia; 2014. Available from: http://hdl.handle.net/10724/25562

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Georgia

28. Johnston, Michael Alexander. Inhibition of Escherichia coli O157:H7 by background microflora of fresh-cut lettuce and baby spinach.

Degree: 2014, University of Georgia

URL: http://hdl.handle.net/10724/25214

► Escherichia coli O157:H7 contamination of fresh-cut lettuce and spinach can occur from pre-harvest to product packaging. Natural microflora present on fresh produce may help reduce… (more)

Subjects/Keywords: Lettuce; spinach; fresh-cut; inhibition; Escherichia coli O157:H7; antagonistic

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APA (6^th Edition):

Johnston, M. A. (2014). Inhibition of Escherichia coli O157:H7 by background microflora of fresh-cut lettuce and baby spinach. (Thesis). University of Georgia. Retrieved from http://hdl.handle.net/10724/25214

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Johnston, Michael Alexander. “Inhibition of Escherichia coli O157:H7 by background microflora of fresh-cut lettuce and baby spinach.” 2014. Thesis, University of Georgia. Accessed April 11, 2021. http://hdl.handle.net/10724/25214.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Johnston, Michael Alexander. “Inhibition of Escherichia coli O157:H7 by background microflora of fresh-cut lettuce and baby spinach.” 2014. Web. 11 Apr 2021.

Vancouver:

Johnston MA. Inhibition of Escherichia coli O157:H7 by background microflora of fresh-cut lettuce and baby spinach. [Internet] [Thesis]. University of Georgia; 2014. [cited 2021 Apr 11]. Available from: http://hdl.handle.net/10724/25214.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Johnston MA. Inhibition of Escherichia coli O157:H7 by background microflora of fresh-cut lettuce and baby spinach. [Thesis]. University of Georgia; 2014. Available from: http://hdl.handle.net/10724/25214

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Texas A&M University

29. Adams, Edward Blair. Characterization and identification of two virus diseases of spinach in South Texas.

Degree: MS, plant pathology, 2012, Texas A&M University

URL: http://hdl.handle.net/1969.1/ETD-TAMU-1979-THESIS-A211

Subjects/Keywords: plant pathology.; Major plant pathology.; Spinach - Diseases and pests.; Spinach - Texas - Winter Garden.

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Adams, E. B. (2012). Characterization and identification of two virus diseases of spinach in South Texas. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-1979-THESIS-A211

Chicago Manual of Style (16^th Edition):

Adams, Edward Blair. “Characterization and identification of two virus diseases of spinach in South Texas.” 2012. Masters Thesis, Texas A&M University. Accessed April 11, 2021. http://hdl.handle.net/1969.1/ETD-TAMU-1979-THESIS-A211.

MLA Handbook (7^th Edition):

Adams, Edward Blair. “Characterization and identification of two virus diseases of spinach in South Texas.” 2012. Web. 11 Apr 2021.

Vancouver:

Adams EB. Characterization and identification of two virus diseases of spinach in South Texas. [Internet] [Masters thesis]. Texas A&M University; 2012. [cited 2021 Apr 11]. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-1979-THESIS-A211.

Council of Science Editors:

Adams EB. Characterization and identification of two virus diseases of spinach in South Texas. [Masters Thesis]. Texas A&M University; 2012. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-1979-THESIS-A211

University of South Africa

30. Nemadodzi, Lufuno Ethel. Growth and development of baby spinach (Spinacia oleracea L.) with reference to mineral nutrition .

Degree: 2015, University of South Africa

URL: http://hdl.handle.net/10500/18673

► Baby spinach is a relatively new crop in South Africa with both commercial significance and reputed health benefits. It is known to assist in combating… (more)

Subjects/Keywords: Baby spinach; Biomass; Chlorophyll content; Dry matter; Mineral nutrition; Nitrate; Stomatal conductance

Record Details Similar Records Cite « Share

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Nemadodzi, L. E. (2015). Growth and development of baby spinach (Spinacia oleracea L.) with reference to mineral nutrition . (Masters Thesis). University of South Africa. Retrieved from http://hdl.handle.net/10500/18673

Chicago Manual of Style (16^th Edition):

Nemadodzi, Lufuno Ethel. “Growth and development of baby spinach (Spinacia oleracea L.) with reference to mineral nutrition .” 2015. Masters Thesis, University of South Africa. Accessed April 11, 2021. http://hdl.handle.net/10500/18673.

MLA Handbook (7^th Edition):

Nemadodzi, Lufuno Ethel. “Growth and development of baby spinach (Spinacia oleracea L.) with reference to mineral nutrition .” 2015. Web. 11 Apr 2021.

Vancouver:

Nemadodzi LE. Growth and development of baby spinach (Spinacia oleracea L.) with reference to mineral nutrition . [Internet] [Masters thesis]. University of South Africa; 2015. [cited 2021 Apr 11]. Available from: http://hdl.handle.net/10500/18673.

Council of Science Editors:

Nemadodzi LE. Growth and development of baby spinach (Spinacia oleracea L.) with reference to mineral nutrition . [Masters Thesis]. University of South Africa; 2015. Available from: http://hdl.handle.net/10500/18673

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Open Access Theses and Dissertations