subject:(Spermatozoa)

Oregon State University

1. Dille, Kenneth Leroy. The hydrolysis of desoxyribonucleic acid derived from salmon sperm.

Degree: MS, Chemistry, 1952, Oregon State University

URL: http://hdl.handle.net/1957/52561

Subjects/Keywords: Spermatozoa

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APA (6^th Edition):

Dille, K. L. (1952). The hydrolysis of desoxyribonucleic acid derived from salmon sperm. (Masters Thesis). Oregon State University. Retrieved from http://hdl.handle.net/1957/52561

Chicago Manual of Style (16^th Edition):

Dille, Kenneth Leroy. “The hydrolysis of desoxyribonucleic acid derived from salmon sperm.” 1952. Masters Thesis, Oregon State University. Accessed April 03, 2020. http://hdl.handle.net/1957/52561.

MLA Handbook (7^th Edition):

Dille, Kenneth Leroy. “The hydrolysis of desoxyribonucleic acid derived from salmon sperm.” 1952. Web. 03 Apr 2020.

Vancouver:

Dille KL. The hydrolysis of desoxyribonucleic acid derived from salmon sperm. [Internet] [Masters thesis]. Oregon State University; 1952. [cited 2020 Apr 03]. Available from: http://hdl.handle.net/1957/52561.

Council of Science Editors:

Dille KL. The hydrolysis of desoxyribonucleic acid derived from salmon sperm. [Masters Thesis]. Oregon State University; 1952. Available from: http://hdl.handle.net/1957/52561

University of Hong Kong

2. Tsoi, Sin-yi. The biological effects of prostaglandins on human sperm function.

Degree: Master of Medical Sciences, 2017, University of Hong Kong

URL: http://hdl.handle.net/10722/252478

►

Prostaglandins are a group of essential hormone-like eicosanoids that play an important role in inflammatory processes in the human body. They have been found in… (more)

Subjects/Keywords: Spermatozoa; Prostaglandins

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APA (6^th Edition):

Tsoi, S. (2017). The biological effects of prostaglandins on human sperm function. (Masters Thesis). University of Hong Kong. Retrieved from http://hdl.handle.net/10722/252478

Chicago Manual of Style (16^th Edition):

Tsoi, Sin-yi. “The biological effects of prostaglandins on human sperm function.” 2017. Masters Thesis, University of Hong Kong. Accessed April 03, 2020. http://hdl.handle.net/10722/252478.

MLA Handbook (7^th Edition):

Tsoi, Sin-yi. “The biological effects of prostaglandins on human sperm function.” 2017. Web. 03 Apr 2020.

Vancouver:

Tsoi S. The biological effects of prostaglandins on human sperm function. [Internet] [Masters thesis]. University of Hong Kong; 2017. [cited 2020 Apr 03]. Available from: http://hdl.handle.net/10722/252478.

Council of Science Editors:

Tsoi S. The biological effects of prostaglandins on human sperm function. [Masters Thesis]. University of Hong Kong; 2017. Available from: http://hdl.handle.net/10722/252478

Oregon State University

3. Graybill, James Roger. Cryo-preservation of viable fish sperm.

Degree: MS, Fisheries and Wildlife, 1968, Oregon State University

URL: http://hdl.handle.net/1957/22204

► Fishery researchers have attempted to preserve viable fish sperm for extended periods of time. Only limited success has been achieved with storage of spermatozoa from… (more)

▼ Fishery researchers have attempted to preserve viable fish sperm for extended periods of time. Only limited success has been achieved with storage of spermatozoa from salmonids. The principal accomplishments of this investigation during the fiscal year 1966-1967 were the development of standard methods for the collection and evaluation of fish sperm, the development of an extender and life protector of spermatozoa, and the development of a freezing and thawing procedure for the live preservation of fish sperm at temperatures of liquid nitrogen (-196 C). Collection of fish sperm was accomplished by means of a suction device with which semen was withdrawn from the genital pore of a fish into a test tube. A modification of the conventional hemocytometric technique was used to determine the concentration of sperm in fish semen. The use of vital stains to differentiate between live and dead sperm cells was investigated and abandoned due to inconsistent results. A subjective method of estimating the viability and relative motility of spermatozoa was established. Experiments were conducted to prolong the viability of sperm cells in the unfrozen state. The deleterious effect of water on prolonging the viability of fish sperm was confirmed. I found that a high percentage of sperm cells remained viable for at least three days if they were refrigerated under aerobic conditions and kept from contact with water. Increased dilution of fish semen with extenders reduced the viability of sperm cells. The freezing point depression of solutions isotonic to fish spermatozoa was found to be -0.51 C. My evidence suggests that the key to maintaining viable fish sperm at 5 C is to provide substances in the extender which can be aerobically metabolized and stored as latent energy by the spermatozoa. Experiments were conducted to prolong the viability of fish spermatozoa in the frozen state. Dimethyl sulfoxide (DMS0) gave the best results of the life protectors studied. Equilibration of the sperm cells in increasing concentrations of a life protector may have increased the viability of the spermatozoa. During several experiments, the optimum concentration of protector increased as the rate of freezing increased. The best survival of viable sperm was obtained in samples that were frozen in Solution 48 in combination with DMSO as a protector. Thawing the ampoules of semen in a 4 C water bath yielded the highest recovery of viable spermatozoa. Alevins were produced from eggs fertilized with cryogenically preserved fish sperm. These alevins appeared to be as normal as young fish produced from unfrozen spermatozoa. The fertility rate was low (0-18%), but to my knowledge this is the first successful attempt to fertilize salmonid eggs with cryo-preserved spermatozoa. The findings in this study warrant further investigation. Advisors/Committee Members: Horton, Howard F. (advisor).

Subjects/Keywords: Spermatozoa

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APA (6^th Edition):

Graybill, J. R. (1968). Cryo-preservation of viable fish sperm. (Masters Thesis). Oregon State University. Retrieved from http://hdl.handle.net/1957/22204

Chicago Manual of Style (16^th Edition):

Graybill, James Roger. “Cryo-preservation of viable fish sperm.” 1968. Masters Thesis, Oregon State University. Accessed April 03, 2020. http://hdl.handle.net/1957/22204.

MLA Handbook (7^th Edition):

Graybill, James Roger. “Cryo-preservation of viable fish sperm.” 1968. Web. 03 Apr 2020.

Vancouver:

Graybill JR. Cryo-preservation of viable fish sperm. [Internet] [Masters thesis]. Oregon State University; 1968. [cited 2020 Apr 03]. Available from: http://hdl.handle.net/1957/22204.

Council of Science Editors:

Graybill JR. Cryo-preservation of viable fish sperm. [Masters Thesis]. Oregon State University; 1968. Available from: http://hdl.handle.net/1957/22204

Oregon State University

4. Lobo, Carlos Alfredo. Effect of rumen fluid upon the preservation of bovine spermatozoa.

Degree: MS, Animal Science, 1966, Oregon State University

URL: http://hdl.handle.net/1957/47705

► Experiments were conducted to determine the effect of rumen fluid on live and motile spermatozoa of semen extended in yolk-citrate and stored at 4°C. Preliminary… (more)

▼ Experiments were conducted to determine the effect of rumen fluid on live and motile spermatozoa of semen extended in yolk-citrate and stored at 4°C. Preliminary work showed that levels of rumen fluid below 10 percent were beneficial in keeping spermatozoan activity up to 14 days in storage. For more revealing information, 1664 observations for live and motility were made on 16 collections from 4 dairy bulls. Semen was extended in 0.5, 1.0 and 5.0 percent boiled or raw rumen fluid, treated or nontreated with 500 I.U. of penicillin and 500 micrograms of dihydrostreptomycin per ml. of extender. From microscopic evaluations to the ninth day, the results were seen to fall into three definite groups: control, antibiotic treated, and nontreated semen. Higher ratings were observed in the lower levels than in the 5 percent level of rumen fluid (p = .001). Additional 768 observations to determine the effect of argon and oxygen on semen extended in different levels of rumen fluid showed highly significant differences in motility, but no differences in percent alive. For the ninth day rating, rumen fluid at 1.0% or lower levels increased motility by 27 percent in comparison with control. Antibiotics decreased this figure to 11 percent. Oxygen decreased motility by 12 percent; however, at the 5 percent level, oxygen rated 25 percent higher than oxygen without rumen fluid. Argon showed an overall decline in motility of 8 percent. It can be concluded that the activity of bovine spermatozoa can be increased with small amounts of rumen fluid. The detrimental effect of antibiotics on motility (p < .0001) and the nonharmful effect of oxygen on the life of the spermatozoa contradict present belief. The fact that boiling did not affect the beneficial effect of rumen fluid suggests sterile means for using rumen fluid in artificial insemination. Since the action of some substance produced by a rumen microorganism is suspected, the possibility of isolating this microorganism can not be overlooked. Advisors/Committee Members: Wolberg, Floyd B. (advisor).

Subjects/Keywords: Spermatozoa

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APA (6^th Edition):

Lobo, C. A. (1966). Effect of rumen fluid upon the preservation of bovine spermatozoa. (Masters Thesis). Oregon State University. Retrieved from http://hdl.handle.net/1957/47705

Chicago Manual of Style (16^th Edition):

Lobo, Carlos Alfredo. “Effect of rumen fluid upon the preservation of bovine spermatozoa.” 1966. Masters Thesis, Oregon State University. Accessed April 03, 2020. http://hdl.handle.net/1957/47705.

MLA Handbook (7^th Edition):

Lobo, Carlos Alfredo. “Effect of rumen fluid upon the preservation of bovine spermatozoa.” 1966. Web. 03 Apr 2020.

Vancouver:

Lobo CA. Effect of rumen fluid upon the preservation of bovine spermatozoa. [Internet] [Masters thesis]. Oregon State University; 1966. [cited 2020 Apr 03]. Available from: http://hdl.handle.net/1957/47705.

Council of Science Editors:

Lobo CA. Effect of rumen fluid upon the preservation of bovine spermatozoa. [Masters Thesis]. Oregon State University; 1966. Available from: http://hdl.handle.net/1957/47705

Oregon State University

5. Turner, H. A. Morphology of epididymal spermatozoa from inbred and linecross bulls.

Degree: MS, Animal Science, 1965, Oregon State University

URL: http://hdl.handle.net/1957/47937

► Data were presented on epididymal spermatozoa from 29 inbred and linecross bulls raised at Oregon State University in connection with the Western Regional Beef Cattle… (more)

▼ Data were presented on epididymal spermatozoa from 29 inbred and linecross bulls raised at Oregon State University in connection with the Western Regional Beef Cattle Breeding Project, W-1. The inbred bulls came from four inbred lines. Three of these inbred lines were Herfords and the other line was Angus. The 21 linecross bulls were produced from reciprocal crosses of the three Hereford lines. The bulls were put on test at 450 pounds. They were fed one part concentrate to two parts roughage up to 800 pounds at which time the concentrates were increased. They were slaughtered at 1000 pounds. The epididymides were stripped from the testes at the time of slaughter and sperm were obtained from the head and tail of the epididymides for making slides. An eosin-fast green differential stain was used to make slides for characterizing the spermatozoa as dead or alive and for detecting physiological abnormalities. Two slides were made from each location on the epididymis and labeled as head or tail and left or right. This made 16 slides per bull. The slides were then studied and 200 spermatozoa were counted on each slide. Only ten to 25 spermatozoa were counted in one spot in order to obtain an overall picture of each slide. The number of live, dead, normal, and types of abnormals were recorded. The different types of abnormals counted and recorded were neck beads, tail beads, tailless heads, coiled tails, and bent tails. An analysis of variance was run for breeding types, sides, and locations and all possible interactions between these variables. Statistical significance was determined by use of the F test. Means were calculated for breeding types and locations. To estimate general combining ability effects, means of lines I, II, or III combined with each of the other two lines are compared. To estimate specific combining ability, means of I x II, I x III, and II x III are compared. The analysis of variance indicated that there were statistically significant differences between breeding types for live spermatozoa, tail beads, and bent tails. Since a particular breeding type that was superior in one characteristic was also inferior in another, there was no generally superior breeding type. Advisors/Committee Members: Bogart, Ralph (advisor).

Subjects/Keywords: Spermatozoa

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APA (6^th Edition):

Turner, H. A. (1965). Morphology of epididymal spermatozoa from inbred and linecross bulls. (Masters Thesis). Oregon State University. Retrieved from http://hdl.handle.net/1957/47937

Chicago Manual of Style (16^th Edition):

Turner, H A. “Morphology of epididymal spermatozoa from inbred and linecross bulls.” 1965. Masters Thesis, Oregon State University. Accessed April 03, 2020. http://hdl.handle.net/1957/47937.

MLA Handbook (7^th Edition):

Turner, H A. “Morphology of epididymal spermatozoa from inbred and linecross bulls.” 1965. Web. 03 Apr 2020.

Vancouver:

Turner HA. Morphology of epididymal spermatozoa from inbred and linecross bulls. [Internet] [Masters thesis]. Oregon State University; 1965. [cited 2020 Apr 03]. Available from: http://hdl.handle.net/1957/47937.

Council of Science Editors:

Turner HA. Morphology of epididymal spermatozoa from inbred and linecross bulls. [Masters Thesis]. Oregon State University; 1965. Available from: http://hdl.handle.net/1957/47937

Oregon State University

6. Das, Jogananda. Effects of five different levels of milk fat on the life span of bovine spermatozoa stored in milk diluents.

Degree: MS, Dairy and Animal Husbandry, 1960, Oregon State University

URL: http://hdl.handle.net/1957/50272

Subjects/Keywords: Spermatozoa

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APA (6^th Edition):

Das, J. (1960). Effects of five different levels of milk fat on the life span of bovine spermatozoa stored in milk diluents. (Masters Thesis). Oregon State University. Retrieved from http://hdl.handle.net/1957/50272

Chicago Manual of Style (16^th Edition):

Das, Jogananda. “Effects of five different levels of milk fat on the life span of bovine spermatozoa stored in milk diluents.” 1960. Masters Thesis, Oregon State University. Accessed April 03, 2020. http://hdl.handle.net/1957/50272.

MLA Handbook (7^th Edition):

Das, Jogananda. “Effects of five different levels of milk fat on the life span of bovine spermatozoa stored in milk diluents.” 1960. Web. 03 Apr 2020.

Vancouver:

Das J. Effects of five different levels of milk fat on the life span of bovine spermatozoa stored in milk diluents. [Internet] [Masters thesis]. Oregon State University; 1960. [cited 2020 Apr 03]. Available from: http://hdl.handle.net/1957/50272.

Council of Science Editors:

Das J. Effects of five different levels of milk fat on the life span of bovine spermatozoa stored in milk diluents. [Masters Thesis]. Oregon State University; 1960. Available from: http://hdl.handle.net/1957/50272

Oregon State University

7. Kliewer, Ray Henry. Gas-pressure technique for the preservation of bovine spermatozoa.

Degree: MS, Dairy and Animal Husbandry, 1960, Oregon State University

URL: http://hdl.handle.net/1957/50428

Subjects/Keywords: Spermatozoa

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APA (6^th Edition):

Kliewer, R. H. (1960). Gas-pressure technique for the preservation of bovine spermatozoa. (Masters Thesis). Oregon State University. Retrieved from http://hdl.handle.net/1957/50428

Chicago Manual of Style (16^th Edition):

Kliewer, Ray Henry. “Gas-pressure technique for the preservation of bovine spermatozoa.” 1960. Masters Thesis, Oregon State University. Accessed April 03, 2020. http://hdl.handle.net/1957/50428.

MLA Handbook (7^th Edition):

Kliewer, Ray Henry. “Gas-pressure technique for the preservation of bovine spermatozoa.” 1960. Web. 03 Apr 2020.

Vancouver:

Kliewer RH. Gas-pressure technique for the preservation of bovine spermatozoa. [Internet] [Masters thesis]. Oregon State University; 1960. [cited 2020 Apr 03]. Available from: http://hdl.handle.net/1957/50428.

Council of Science Editors:

Kliewer RH. Gas-pressure technique for the preservation of bovine spermatozoa. [Masters Thesis]. Oregon State University; 1960. Available from: http://hdl.handle.net/1957/50428

Oregon State University

8. Ott, Alvin G. Fertilization of salmonid eggs with cryopreserved sperm.

Degree: MS, Fisheries and Wildlife, 1969, Oregon State University

URL: http://hdl.handle.net/1957/22561

► Numerous fishery researchers have attempted to preserve viable fish sperm, but only three investigations were successful in the fertilization of fresh eggs with frozen and… (more)

Subjects/Keywords: Spermatozoa

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APA (6^th Edition):

Ott, A. G. (1969). Fertilization of salmonid eggs with cryopreserved sperm. (Masters Thesis). Oregon State University. Retrieved from http://hdl.handle.net/1957/22561

Chicago Manual of Style (16^th Edition):

Ott, Alvin G. “Fertilization of salmonid eggs with cryopreserved sperm.” 1969. Masters Thesis, Oregon State University. Accessed April 03, 2020. http://hdl.handle.net/1957/22561.

MLA Handbook (7^th Edition):

Ott, Alvin G. “Fertilization of salmonid eggs with cryopreserved sperm.” 1969. Web. 03 Apr 2020.

Vancouver:

Ott AG. Fertilization of salmonid eggs with cryopreserved sperm. [Internet] [Masters thesis]. Oregon State University; 1969. [cited 2020 Apr 03]. Available from: http://hdl.handle.net/1957/22561.

Council of Science Editors:

Ott AG. Fertilization of salmonid eggs with cryopreserved sperm. [Masters Thesis]. Oregon State University; 1969. Available from: http://hdl.handle.net/1957/22561

University of Waikato

9. Beattie, Cleo. The Effects of Different Cryoprotectants on Stallion Epididymal Spermatozoa .

Degree: 2011, University of Waikato

URL: http://hdl.handle.net/10289/5597

► Any event that makes semen collection or mating impossible, such as death, castration, or injury, may terminate a stallion’s breeding career. Fortunately, stallion sperm which… (more)

▼ Any event that makes semen collection or mating impossible, such as death, castration, or injury, may terminate a stallion’s breeding career. Fortunately, stallion sperm which are capable of fertilization can be harvested from a region adjacent to the testicles called the epididymis, and frozen for future use. However, the fertility of frozen-thawed epididymal sperm has been found to be lower than that of ejaculated sperm. This thesis investigated the effects of different cryoprotectants and dilution after thawing with SW3 media (SW3, JEL Media, NZ), which contains complex amino acids, sugars and no protein, on the fertility and sperm characteristics of frozen-thawed epididymal sperm. Epididymal sperm were obtained from 5 colts at the time of castration and each collected sample was split into different treatment groups and frozen with different cryoprotectants (glycerol, formamides or a combination) in the same base extender. The sperm were frozen in liquid Nitrogen until the time of insemination or post-thaw analysis. The effects of different cryoprotectants and the addition of the post-thaw dilution medium on motility parameters, morphology, acrosome status, plasma membrane integrity and DNA integrity were evaluated after thawing. The reproductive tract of mares were examined by ultrasound during oestrous and the mares were inseminated at 4 hours prior to ovulation with epididymal sperm frozen in either glycerol or a glycerol and formamide combination. Fertility was determined at either 8 days after ovulation when the uterus of each mare was flushed for an embryo or at 14 days after ovulation when the mare was scanned to determine if there was a pregnancy. The post-thaw dilution medium (SW3) was found to have a significant beneficial effect on the motility of frozen-thawed epididymal sperm frozen with glycerol in vitro. Pregnancy data revealed that the glycerol and formamide combination cryoprotectant was superior to glycerol alone, with 50% versus 10% pregnancy rates recorded respectively. These results suggest that the higher pregnancy rates are the effect of the cryoprotectant and not the complex amino acids of the base extender (which was the same for all samples) which contribute to the improved fertility of the frozen epididymal sperm. Furthermore, because good pregnancy rates without the addition of SW3 were obtained prior to insemination, this supports the conclusion that the cryoprotectant is a key factor altering the fertilizing ability of epididymal sperm. Investigations into the fertility of epididymal sperm will enhance our understanding of factors which affect sperm fertility whilst in storage in the cauda epididymius. Furthermore, the opportunity to preserve the fertility of these gametes at the time of gelding or death of a stallion will have long-lasting beneficial effects on the size of the gene pool for selection. This is not only important for the domestic horse but for endangered equine species also. Ultimately, the fertility of epididymal sperm should be optimised to ensure that the maximum… Advisors/Committee Members: Molan, Peter C (advisor), Cursons, Raymond T (advisor).

Subjects/Keywords: Epididymal; Spermatozoa

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APA (6^th Edition):

Beattie, C. (2011). The Effects of Different Cryoprotectants on Stallion Epididymal Spermatozoa . (Masters Thesis). University of Waikato. Retrieved from http://hdl.handle.net/10289/5597

Chicago Manual of Style (16^th Edition):

Beattie, Cleo. “The Effects of Different Cryoprotectants on Stallion Epididymal Spermatozoa .” 2011. Masters Thesis, University of Waikato. Accessed April 03, 2020. http://hdl.handle.net/10289/5597.

MLA Handbook (7^th Edition):

Beattie, Cleo. “The Effects of Different Cryoprotectants on Stallion Epididymal Spermatozoa .” 2011. Web. 03 Apr 2020.

Vancouver:

Beattie C. The Effects of Different Cryoprotectants on Stallion Epididymal Spermatozoa . [Internet] [Masters thesis]. University of Waikato; 2011. [cited 2020 Apr 03]. Available from: http://hdl.handle.net/10289/5597.

Council of Science Editors:

Beattie C. The Effects of Different Cryoprotectants on Stallion Epididymal Spermatozoa . [Masters Thesis]. University of Waikato; 2011. Available from: http://hdl.handle.net/10289/5597

Michigan State University

10. Myszewski, Michael Edward. Differential survival among sperm populations from heterozygous male Drosophila melanogaster.

Degree: PhD, Department of Zoology, 1965, Michigan State University

URL: http://etd.lib.msu.edu/islandora/object/etd:41573

Subjects/Keywords: Spermatozoa

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APA (6^th Edition):

Myszewski, M. E. (1965). Differential survival among sperm populations from heterozygous male Drosophila melanogaster. (Doctoral Dissertation). Michigan State University. Retrieved from http://etd.lib.msu.edu/islandora/object/etd:41573

Chicago Manual of Style (16^th Edition):

Myszewski, Michael Edward. “Differential survival among sperm populations from heterozygous male Drosophila melanogaster.” 1965. Doctoral Dissertation, Michigan State University. Accessed April 03, 2020. http://etd.lib.msu.edu/islandora/object/etd:41573.

MLA Handbook (7^th Edition):

Myszewski, Michael Edward. “Differential survival among sperm populations from heterozygous male Drosophila melanogaster.” 1965. Web. 03 Apr 2020.

Vancouver:

Myszewski ME. Differential survival among sperm populations from heterozygous male Drosophila melanogaster. [Internet] [Doctoral dissertation]. Michigan State University; 1965. [cited 2020 Apr 03]. Available from: http://etd.lib.msu.edu/islandora/object/etd:41573.

Council of Science Editors:

Myszewski ME. Differential survival among sperm populations from heterozygous male Drosophila melanogaster. [Doctoral Dissertation]. Michigan State University; 1965. Available from: http://etd.lib.msu.edu/islandora/object/etd:41573

University of Hawaii – Manoa

11. Smith, Todd Timothy. The biology of mammalian spermatozoa in the oviduct.

Degree: PhD, 2009, University of Hawaii – Manoa

URL: http://hdl.handle.net/10125/9361

►

Typescript.

Microfiche.

ix, 109 leaves, bound ill. 29 cm

The oviduct occupies a unique position in mammalian reproduction as the site of sperm transport, the… (more)

Subjects/Keywords: Mammals – Spermatozoa; Oviduct

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APA (6^th Edition):

Smith, T. T. (2009). The biology of mammalian spermatozoa in the oviduct. (Doctoral Dissertation). University of Hawaii – Manoa. Retrieved from http://hdl.handle.net/10125/9361

Chicago Manual of Style (16^th Edition):

Smith, Todd Timothy. “The biology of mammalian spermatozoa in the oviduct.” 2009. Doctoral Dissertation, University of Hawaii – Manoa. Accessed April 03, 2020. http://hdl.handle.net/10125/9361.

MLA Handbook (7^th Edition):

Smith, Todd Timothy. “The biology of mammalian spermatozoa in the oviduct.” 2009. Web. 03 Apr 2020.

Vancouver:

Smith TT. The biology of mammalian spermatozoa in the oviduct. [Internet] [Doctoral dissertation]. University of Hawaii – Manoa; 2009. [cited 2020 Apr 03]. Available from: http://hdl.handle.net/10125/9361.

Council of Science Editors:

Smith TT. The biology of mammalian spermatozoa in the oviduct. [Doctoral Dissertation]. University of Hawaii – Manoa; 2009. Available from: http://hdl.handle.net/10125/9361

University of Hong Kong

12. Leung, Tsz-ying. Vitrification of human spermatozoa : the effects of antioxidant supplementation on post-warmed sperm parameters.

Degree: Master of Medical Sciences, 2017, University of Hong Kong

URL: http://hdl.handle.net/10722/251353

►

Human sperm cryopreservation provides an option to male patients who wish to retain fertility for medical or personal reasons. Though the development of this technique… (more)

▼

Human sperm cryopreservation provides an option to male patients who wish to retain fertility for medical or personal reasons. Though the development of this technique benefits patients in need, some technical issues related to oxidative damages remain unresolved. Excessive reactive oxygen species (ROS) induced by cryopreservation can exceed the endogenous antioxidant capacity resulting in oxidative stress. The vulnerability of spermatozoa to oxidative damages can result in severe impairment of sperm parameters. The hypothesis of this study was that the supplementation of vitrification medium with exogenous antioxidants protect human spermatozoa against oxidative damages and subsequently improve the post-warmed parameters. The first objective was to evaluate the effects of vitamin E, spermine, vitamin C and lycopene on the pre-vitrified spermatozoa; and identify the optimal concentrations of each treatment group prior to vitrification. The treatment groups of vitamin C at 25, 50, 100 μM, vitamin E at 250, 500, 1000 μM, spermine at 125, 250, 500 μM and lycopene at 1.5, 3.0, 6.0 μM demonstrated no adverse effects on the sperm parameters in terms of motility, viability and capability to undergo acrosome reaction induced by calcium ionophore A23187. The preliminary data of this study demonstrated the detrimental impacts of cryopreservation on post-warmed sperm parameters. The comparison between the pre-vitrified and post-warmed parameters revealed that cryopreservation reduced the post-warmed progressive motility by more than 80% compared to the control. The second objective was to examine whether supplementation of the vitrification medium with vitamin E, spermine, vitamin C and lycopene could improve the post-warmed sperm parameters in terms of motility, viability, capability to undergo acrosome reaction induced by calcium ionophore A23187 and DNA fragmentation. In conclusion, the results of this study validated the protective effects of exogenous antioxidants on human spermatozoa. Vitamin C and spermine respectively improved the post-warmed parameters in terms of progressive motility, viability, acrosomal integrity and DNA fragmentation of vitrified spermatozoa. The use of these antioxidants could potentially protect human spermatozoa against oxidative damages induced by cryopreservation and subsequently improve the fertilizing ability.

published_or_final_version

Obstetrics and Gynaecology

Master

Master of Medical Sciences

Subjects/Keywords: Antioxidants; Spermatozoa - Cryopreservation

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APA (6^th Edition):

Leung, T. (2017). Vitrification of human spermatozoa : the effects of antioxidant supplementation on post-warmed sperm parameters. (Masters Thesis). University of Hong Kong. Retrieved from http://hdl.handle.net/10722/251353

Chicago Manual of Style (16^th Edition):

Leung, Tsz-ying. “Vitrification of human spermatozoa : the effects of antioxidant supplementation on post-warmed sperm parameters.” 2017. Masters Thesis, University of Hong Kong. Accessed April 03, 2020. http://hdl.handle.net/10722/251353.

MLA Handbook (7^th Edition):

Leung, Tsz-ying. “Vitrification of human spermatozoa : the effects of antioxidant supplementation on post-warmed sperm parameters.” 2017. Web. 03 Apr 2020.

Vancouver:

Leung T. Vitrification of human spermatozoa : the effects of antioxidant supplementation on post-warmed sperm parameters. [Internet] [Masters thesis]. University of Hong Kong; 2017. [cited 2020 Apr 03]. Available from: http://hdl.handle.net/10722/251353.

Council of Science Editors:

Leung T. Vitrification of human spermatozoa : the effects of antioxidant supplementation on post-warmed sperm parameters. [Masters Thesis]. University of Hong Kong; 2017. Available from: http://hdl.handle.net/10722/251353

University of Hong Kong

13. Yu, Oi-yan. A study of p53-binding protein 1 in sperm function.

Degree: Master of Medical Sciences, 2013, University of Hong Kong

URL: Yu, O. [余靄恩]. (2013). A study of p53-binding protein 1 in sperm function. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5091616 ; http://dx.doi.org/10.5353/th_b5091616 ; http://hdl.handle.net/10722/193550

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Spermatozoa experience a series of physiological alternation upon traveling from the testis to the epididymis in order to acquire motility and capacity necessary to recognize… (more)

Subjects/Keywords: Carrier proteins; Spermatozoa

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Yu, O. (2013). A study of p53-binding protein 1 in sperm function. (Masters Thesis). University of Hong Kong. Retrieved from Yu, O. [余靄恩]. (2013). A study of p53-binding protein 1 in sperm function. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5091616 ; http://dx.doi.org/10.5353/th_b5091616 ; http://hdl.handle.net/10722/193550

Chicago Manual of Style (16^th Edition):

Yu, Oi-yan. “A study of p53-binding protein 1 in sperm function.” 2013. Masters Thesis, University of Hong Kong. Accessed April 03, 2020. Yu, O. [余靄恩]. (2013). A study of p53-binding protein 1 in sperm function. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5091616 ; http://dx.doi.org/10.5353/th_b5091616 ; http://hdl.handle.net/10722/193550.

MLA Handbook (7^th Edition):

Yu, Oi-yan. “A study of p53-binding protein 1 in sperm function.” 2013. Web. 03 Apr 2020.

Vancouver:

Yu O. A study of p53-binding protein 1 in sperm function. [Internet] [Masters thesis]. University of Hong Kong; 2013. [cited 2020 Apr 03]. Available from: Yu, O. [余靄恩]. (2013). A study of p53-binding protein 1 in sperm function. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5091616 ; http://dx.doi.org/10.5353/th_b5091616 ; http://hdl.handle.net/10722/193550.

Council of Science Editors:

Yu O. A study of p53-binding protein 1 in sperm function. [Masters Thesis]. University of Hong Kong; 2013. Available from: Yu, O. [余靄恩]. (2013). A study of p53-binding protein 1 in sperm function. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5091616 ; http://dx.doi.org/10.5353/th_b5091616 ; http://hdl.handle.net/10722/193550

University of Hong Kong

14. Huang, Wenxin. Sperm fucosyltransferase-5 mediates the sperm-oviductal epithelial cell interaction to protect human sperm from oxidative damage.

Degree: PhD, 2013, University of Hong Kong

URL: Huang, W. [黃聞馨]. (2013). Sperm fucosyltransferase-5 mediates the sperm-oviductal epithelial cell interaction to protect human sperm from oxidative damage. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5177296 ; http://dx.doi.org/10.5353/th_b5177296 ; http://hdl.handle.net/10722/196485

► Oxidative damage by reactive oxygen species (ROS) is a major cause of sperm dysfunction. Excessive ROS generation reduces fertilization and enhances DNA damage of spermatozoa.… (more)

▼ Oxidative damage by reactive oxygen species (ROS) is a major cause of sperm dysfunction. Excessive ROS generation reduces fertilization and enhances DNA damage of spermatozoa. In mammals, including humans, oviduct functions as a sperm reservoir which is created by the binding of spermatozoa to the epithelial lining in the oviduct. Interaction between sperm and oviductal epithelial cells improves the fertilizing ability of and reduces chromatin damage in spermatozoa. However, the mechanism(s) by which spermatozoa-oviduct interaction producing these beneficial effects is unknown. One possibility is that oviduct protects spermatozoa from oxidative stress. The hypothesis of this project was that oviductal cell membrane proteins interact with spermatozoa to protect them from oxidative damage. Due to the limited availability of human oviductal tissue for research, an immortalized human oviductal epithelial cell line, OE-E6/E7, was used as a study model. The first objective examined the effect of OE-E6/E7 membrane proteins on human spermatozoa. The extracted OE-E6/E7 membrane proteins bound to sperm head and preferentially to uncapacitated sperm. Pretreatment with OE-E6/E7 membrane proteins significantly suppressed ROS-induced adverse effects in sperm motility, membrane integrity, DNA integrity, and intracellular ROS level. OE-E6/E7 membrane proteins also increased the endogenous enzyme activities of sperm superoxide dismutase (SOD) and glutathione peroxidase (GPx). Sperm fucosyltransferase-5 (sFUT5) is a membrane carbohydrate-binding protein on human sperm. The second objective investigated the involvement of sFUT5 in sperm-oviduct interaction. Purified sFUT5 bound to OE-E6/E7 cells and anti-FUT5 antibody inhibited this interaction. Pre-absorption of OE-E6/E7 membrane proteins with purified sFUT5 or blocking of sFUT5 on sperm with anti-FUT5 antibody significantly inhibited the protective effects of OE-E6/E7 membrane proteins against ROS-induced damages in spermatozoa. Asialofetuin, a reported sFUT5 substrate, can partly mimic the protective effect of OE-E6/E7 membrane proteins. Sperm processing in assisted reproductive technology (ART) treatment, including centrifugation and cryopreservation, has shown to induce ROS production and oxidative damage in sperm. The third objective investigated the possible use of OE-E6/E7 membrane proteins or asialofetuin as an antioxidant supplement during centrifugation and cryopreservation. No adverse effect on sperm functions was detected by centrifugation using our centrifugation protocols. OE-E6/E7 membrane proteins or asialofetuin pretreatment suppressed the cryopreservation-induced damage on sperm in terms of motility and DNA fragmentation. The fourth objective aimed to identify the sFUT5-interacting proteins from OE-E6/E7 membrane extracts. By using immuno-affinity chromatography and mass spectrometry analysis, cell adhesion molecule 4 (CADM4) was identified as a potential sFUT5-interacting protein. This result was further supported by co-immunoprecipitation,… Advisors/Committee Members: Chiu, CN, Ho, PC, Pang, RTK.

Subjects/Keywords: Spermatozoa; Membrane proteins

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Huang, W. (2013). Sperm fucosyltransferase-5 mediates the sperm-oviductal epithelial cell interaction to protect human sperm from oxidative damage. (Doctoral Dissertation). University of Hong Kong. Retrieved from Huang, W. [黃聞馨]. (2013). Sperm fucosyltransferase-5 mediates the sperm-oviductal epithelial cell interaction to protect human sperm from oxidative damage. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5177296 ; http://dx.doi.org/10.5353/th_b5177296 ; http://hdl.handle.net/10722/196485

Chicago Manual of Style (16^th Edition):

Huang, Wenxin. “Sperm fucosyltransferase-5 mediates the sperm-oviductal epithelial cell interaction to protect human sperm from oxidative damage.” 2013. Doctoral Dissertation, University of Hong Kong. Accessed April 03, 2020. Huang, W. [黃聞馨]. (2013). Sperm fucosyltransferase-5 mediates the sperm-oviductal epithelial cell interaction to protect human sperm from oxidative damage. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5177296 ; http://dx.doi.org/10.5353/th_b5177296 ; http://hdl.handle.net/10722/196485.

MLA Handbook (7^th Edition):

Huang, Wenxin. “Sperm fucosyltransferase-5 mediates the sperm-oviductal epithelial cell interaction to protect human sperm from oxidative damage.” 2013. Web. 03 Apr 2020.

Vancouver:

Huang W. Sperm fucosyltransferase-5 mediates the sperm-oviductal epithelial cell interaction to protect human sperm from oxidative damage. [Internet] [Doctoral dissertation]. University of Hong Kong; 2013. [cited 2020 Apr 03]. Available from: Huang, W. [黃聞馨]. (2013). Sperm fucosyltransferase-5 mediates the sperm-oviductal epithelial cell interaction to protect human sperm from oxidative damage. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5177296 ; http://dx.doi.org/10.5353/th_b5177296 ; http://hdl.handle.net/10722/196485.

Council of Science Editors:

Huang W. Sperm fucosyltransferase-5 mediates the sperm-oviductal epithelial cell interaction to protect human sperm from oxidative damage. [Doctoral Dissertation]. University of Hong Kong; 2013. Available from: Huang, W. [黃聞馨]. (2013). Sperm fucosyltransferase-5 mediates the sperm-oviductal epithelial cell interaction to protect human sperm from oxidative damage. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5177296 ; http://dx.doi.org/10.5353/th_b5177296 ; http://hdl.handle.net/10722/196485

The Ohio State University

15. Merritt, Thomas Leroy. The oxygen uptake of bovine spermatozoa as affected by freezing, motility rate, level of p[eta], and the addition of bovine cervical mucus.

Degree: PhD, Graduate School, 1960, The Ohio State University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=osu1486479798666028

Subjects/Keywords: Agriculture; Spermatozoa

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APA (6^th Edition):

Merritt, T. L. (1960). The oxygen uptake of bovine spermatozoa as affected by freezing, motility rate, level of p[eta], and the addition of bovine cervical mucus. (Doctoral Dissertation). The Ohio State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=osu1486479798666028

Chicago Manual of Style (16^th Edition):

Merritt, Thomas Leroy. “The oxygen uptake of bovine spermatozoa as affected by freezing, motility rate, level of p[eta], and the addition of bovine cervical mucus.” 1960. Doctoral Dissertation, The Ohio State University. Accessed April 03, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=osu1486479798666028.

MLA Handbook (7^th Edition):

Merritt, Thomas Leroy. “The oxygen uptake of bovine spermatozoa as affected by freezing, motility rate, level of p[eta], and the addition of bovine cervical mucus.” 1960. Web. 03 Apr 2020.

Vancouver:

Merritt TL. The oxygen uptake of bovine spermatozoa as affected by freezing, motility rate, level of p[eta], and the addition of bovine cervical mucus. [Internet] [Doctoral dissertation]. The Ohio State University; 1960. [cited 2020 Apr 03]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1486479798666028.

Council of Science Editors:

Merritt TL. The oxygen uptake of bovine spermatozoa as affected by freezing, motility rate, level of p[eta], and the addition of bovine cervical mucus. [Doctoral Dissertation]. The Ohio State University; 1960. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1486479798666028

The Ohio State University

16. Martig, Robert Clair. The effect of added liproproteins on fertilizing capacity of frozen bovine spermatozoa / y Robert Clair Martig.

Degree: PhD, Graduate School, 1961, The Ohio State University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=osu1486482884007339

Subjects/Keywords: Biology; Spermatozoa

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APA (6^th Edition):

Martig, R. C. (1961). The effect of added liproproteins on fertilizing capacity of frozen bovine spermatozoa / y Robert Clair Martig. (Doctoral Dissertation). The Ohio State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=osu1486482884007339

Chicago Manual of Style (16^th Edition):

Martig, Robert Clair. “The effect of added liproproteins on fertilizing capacity of frozen bovine spermatozoa / y Robert Clair Martig.” 1961. Doctoral Dissertation, The Ohio State University. Accessed April 03, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=osu1486482884007339.

MLA Handbook (7^th Edition):

Martig, Robert Clair. “The effect of added liproproteins on fertilizing capacity of frozen bovine spermatozoa / y Robert Clair Martig.” 1961. Web. 03 Apr 2020.

Vancouver:

Martig RC. The effect of added liproproteins on fertilizing capacity of frozen bovine spermatozoa / y Robert Clair Martig. [Internet] [Doctoral dissertation]. The Ohio State University; 1961. [cited 2020 Apr 03]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1486482884007339.

Council of Science Editors:

Martig RC. The effect of added liproproteins on fertilizing capacity of frozen bovine spermatozoa / y Robert Clair Martig. [Doctoral Dissertation]. The Ohio State University; 1961. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1486482884007339

The Ohio State University

17. Weseli, Donald Fenton. The adenosinetriphosphatase activity of bovine spermatozoa .

Degree: PhD, Graduate School, 1958, The Ohio State University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=osu148656276944249

Subjects/Keywords: Agriculture; Spermatozoa

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APA (6^th Edition):

Weseli, D. F. (1958). The adenosinetriphosphatase activity of bovine spermatozoa . (Doctoral Dissertation). The Ohio State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=osu148656276944249

Chicago Manual of Style (16^th Edition):

Weseli, Donald Fenton. “The adenosinetriphosphatase activity of bovine spermatozoa .” 1958. Doctoral Dissertation, The Ohio State University. Accessed April 03, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=osu148656276944249.

MLA Handbook (7^th Edition):

Weseli, Donald Fenton. “The adenosinetriphosphatase activity of bovine spermatozoa .” 1958. Web. 03 Apr 2020.

Vancouver:

Weseli DF. The adenosinetriphosphatase activity of bovine spermatozoa . [Internet] [Doctoral dissertation]. The Ohio State University; 1958. [cited 2020 Apr 03]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu148656276944249.

Council of Science Editors:

Weseli DF. The adenosinetriphosphatase activity of bovine spermatozoa . [Doctoral Dissertation]. The Ohio State University; 1958. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu148656276944249

University of Hawaii

18. Rogers, Beverly Jane. Studies on biochemical changes associated with in vitro capacitation in golden hamster spermatozoa.

Degree: PhD, 2009, University of Hawaii

URL: http://hdl.handle.net/10125/11482

Typescript.

Bibliography: leaves 215-229.

xii, 233 p illus., graphs

Subjects/Keywords: Spermatozoa; Hamsters

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APA (6^th Edition):

Rogers, B. J. (2009). Studies on biochemical changes associated with in vitro capacitation in golden hamster spermatozoa. (Doctoral Dissertation). University of Hawaii. Retrieved from http://hdl.handle.net/10125/11482

Chicago Manual of Style (16^th Edition):

Rogers, Beverly Jane. “Studies on biochemical changes associated with in vitro capacitation in golden hamster spermatozoa.” 2009. Doctoral Dissertation, University of Hawaii. Accessed April 03, 2020. http://hdl.handle.net/10125/11482.

MLA Handbook (7^th Edition):

Rogers, Beverly Jane. “Studies on biochemical changes associated with in vitro capacitation in golden hamster spermatozoa.” 2009. Web. 03 Apr 2020.

Vancouver:

Rogers BJ. Studies on biochemical changes associated with in vitro capacitation in golden hamster spermatozoa. [Internet] [Doctoral dissertation]. University of Hawaii; 2009. [cited 2020 Apr 03]. Available from: http://hdl.handle.net/10125/11482.

Council of Science Editors:

Rogers BJ. Studies on biochemical changes associated with in vitro capacitation in golden hamster spermatozoa. [Doctoral Dissertation]. University of Hawaii; 2009. Available from: http://hdl.handle.net/10125/11482

University of Hawaii – Manoa

19. Chang, Thomas S.K. Studies of a sulfhydryl oxidase from the male reproductive tract : a sperm-protective enzyme.

Degree: PhD, 2009, University of Hawaii – Manoa

URL: http://hdl.handle.net/10125/11483

Typescript.

Photocopy. [S.l. : s.n., 1976]. 29 cm.

Bibliography: leaves 231-243.

Microfilm

xiii, 243 leaves ill

Subjects/Keywords: Sulfhydryl oxidase; Spermatozoa; Enzymes

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Chang, T. S. K. (2009). Studies of a sulfhydryl oxidase from the male reproductive tract : a sperm-protective enzyme. (Doctoral Dissertation). University of Hawaii – Manoa. Retrieved from http://hdl.handle.net/10125/11483

Chicago Manual of Style (16^th Edition):

Chang, Thomas S K. “Studies of a sulfhydryl oxidase from the male reproductive tract : a sperm-protective enzyme.” 2009. Doctoral Dissertation, University of Hawaii – Manoa. Accessed April 03, 2020. http://hdl.handle.net/10125/11483.

MLA Handbook (7^th Edition):

Chang, Thomas S K. “Studies of a sulfhydryl oxidase from the male reproductive tract : a sperm-protective enzyme.” 2009. Web. 03 Apr 2020.

Vancouver:

Chang TSK. Studies of a sulfhydryl oxidase from the male reproductive tract : a sperm-protective enzyme. [Internet] [Doctoral dissertation]. University of Hawaii – Manoa; 2009. [cited 2020 Apr 03]. Available from: http://hdl.handle.net/10125/11483.

Council of Science Editors:

Chang TSK. Studies of a sulfhydryl oxidase from the male reproductive tract : a sperm-protective enzyme. [Doctoral Dissertation]. University of Hawaii – Manoa; 2009. Available from: http://hdl.handle.net/10125/11483

University of Hong Kong

20. 利偉明; Lee, Wai-ming, Will. Ionic basis of sperm motility initiation.

Degree: PhD, 1984, University of Hong Kong

URL: Lee, W. W. [利偉明]. (1984). Ionic basis of sperm motility initiation. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b3123035 ; http://dx.doi.org/10.5353/th_b3123035 ; http://hdl.handle.net/10722/35145

published_or_final_version

Physiology

Doctoral

Doctor of Philosophy

Subjects/Keywords: Spermatozoa - Motility.

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APA (6^th Edition):

利偉明; Lee, Wai-ming, W. (1984). Ionic basis of sperm motility initiation. (Doctoral Dissertation). University of Hong Kong. Retrieved from Lee, W. W. [利偉明]. (1984). Ionic basis of sperm motility initiation. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b3123035 ; http://dx.doi.org/10.5353/th_b3123035 ; http://hdl.handle.net/10722/35145

Chicago Manual of Style (16^th Edition):

利偉明; Lee, Wai-ming, Will. “Ionic basis of sperm motility initiation.” 1984. Doctoral Dissertation, University of Hong Kong. Accessed April 03, 2020. Lee, W. W. [利偉明]. (1984). Ionic basis of sperm motility initiation. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b3123035 ; http://dx.doi.org/10.5353/th_b3123035 ; http://hdl.handle.net/10722/35145.

MLA Handbook (7^th Edition):

利偉明; Lee, Wai-ming, Will. “Ionic basis of sperm motility initiation.” 1984. Web. 03 Apr 2020.

Vancouver:

利偉明; Lee, Wai-ming W. Ionic basis of sperm motility initiation. [Internet] [Doctoral dissertation]. University of Hong Kong; 1984. [cited 2020 Apr 03]. Available from: Lee, W. W. [利偉明]. (1984). Ionic basis of sperm motility initiation. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b3123035 ; http://dx.doi.org/10.5353/th_b3123035 ; http://hdl.handle.net/10722/35145.

Council of Science Editors:

利偉明; Lee, Wai-ming W. Ionic basis of sperm motility initiation. [Doctoral Dissertation]. University of Hong Kong; 1984. Available from: Lee, W. W. [利偉明]. (1984). Ionic basis of sperm motility initiation. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b3123035 ; http://dx.doi.org/10.5353/th_b3123035 ; http://hdl.handle.net/10722/35145

University of Newcastle

21. Reid, Andrew Timothy. Functional characterisation of dynamin in mouse spermatozoa.

Degree: PhD, 2015, University of Newcastle

URL: http://hdl.handle.net/1959.13/1310603

►

Research Doctorate - Doctor of Philosophy (PhD)

World population growth has been increasing exponentially since the 1950’s, highlighting the inadequacies of current methods of contraception… (more)

▼

Research Doctorate - Doctor of Philosophy (PhD)

World population growth has been increasing exponentially since the 1950’s, highlighting the inadequacies of current methods of contraception and the urgent need for new contraceptive measurements. Conversely, although the major driver of this population growth is the large number of unplanned pregnancies in developing countries, male factor infertility is on the rise within the developed world where it is now estimated to afflict 1 in every 20 males. To address these paradoxical problems we need a far better understanding of the mechanisms behind fertilization, with particular focus on the spermatozoon. Such knowledge would enable us to identify new contraceptive targets as well improve diagnosis of the underlying causes of infertility. Two key functions that the spermatozoon must fulfil in order to fertilize in vivo are spermzona pellucida (ZP) binding and acrosomal exocytosis. In order for spermatozoa to obtain the ability to both bind the ZP and undergo a stimulus induced acrosome reaction they must undergo three distinct phases of maturation, namely spermatogenesis, epididymal maturation and capacitation. In the studies described in this thesis we have sought to investigate the role of the large membrane manipulator, dynamin, during sperm maturation as well as investigate its role during key phases of the fertilization cascade. We have found that dynamin is present within the developing acrosome of immature gametes within the testis and is retained in this domain throughout epididymal maturation and capacitation. Via pharmacological inhibition of dynamin we have demonstrated that the GTPase is responsible for regulation of the progesterone induced acrosome reaction and this directly influences the rates of in vitro fertilization. Furthermore, upon receiving this stimulus to acrosome react, dynamin becomes phosphorylated at key serine residues. Subsequent to these findings we investigated these phosphorylation events further and identified the kinase responsible for the key phosphorylation event on serine 774 of dynamin 1 as glycogen synthase kinase 3 (GSK3). We have demonstrated via pharmacological inhibition of GSK3 that its activity is pivotal in controlling the onset of acrosomal exocytosis. Remarkably, via immunoelectron microscopy we have shown the delivery of this dynamin-phosphorylating kinase to sperm within the distal corpus epididymis via novel granular structures referred to as ‘dense bodies’. Finally we have determined that dynamin 1 and dynamin 2, are positioned to play important regulatory roles within the principal cells as well as the lumen of the epididymis. We have provided evidence that these proteins play essential roles in maintaining the composition of the intraluminal milieu across the caput, corpus and cauda epididymis, and thus influence the maturation of spermatozoa. Taken together, these data have demonstrated a previously unappreciated role for dynamin during pivotal stages of spermatogenesis as well as during the epididymal…

Advisors/Committee Members: University of Newcastle. Faculty of Science & Information Technology, School of Environmental and Life Sciences.

Subjects/Keywords: spermatozoa; Dynamin; acrosome reaction

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APA (6^th Edition):

Reid, A. T. (2015). Functional characterisation of dynamin in mouse spermatozoa. (Doctoral Dissertation). University of Newcastle. Retrieved from http://hdl.handle.net/1959.13/1310603

Chicago Manual of Style (16^th Edition):

Reid, Andrew Timothy. “Functional characterisation of dynamin in mouse spermatozoa.” 2015. Doctoral Dissertation, University of Newcastle. Accessed April 03, 2020. http://hdl.handle.net/1959.13/1310603.

MLA Handbook (7^th Edition):

Reid, Andrew Timothy. “Functional characterisation of dynamin in mouse spermatozoa.” 2015. Web. 03 Apr 2020.

Vancouver:

Reid AT. Functional characterisation of dynamin in mouse spermatozoa. [Internet] [Doctoral dissertation]. University of Newcastle; 2015. [cited 2020 Apr 03]. Available from: http://hdl.handle.net/1959.13/1310603.

Council of Science Editors:

Reid AT. Functional characterisation of dynamin in mouse spermatozoa. [Doctoral Dissertation]. University of Newcastle; 2015. Available from: http://hdl.handle.net/1959.13/1310603

University of Johannesburg

22. Vlok, Wynand. Aspekte van die spermatologie van die kleinbekgeelvis, Barbus aeneus (Cyprinidae).

Degree: 2014, University of Johannesburg

URL: http://hdl.handle.net/10210/9674

►

M.Sc. (Zoology)

Milt and blood samples of Aarbus aeneus were collected during November in order to perform chemical analyses of the blood and seminal plasma.… (more)

Subjects/Keywords: Barbus aeneus; Cyprinidae; Fishes - Spermatozoa

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APA (6^th Edition):

Vlok, W. (2014). Aspekte van die spermatologie van die kleinbekgeelvis, Barbus aeneus (Cyprinidae). (Thesis). University of Johannesburg. Retrieved from http://hdl.handle.net/10210/9674

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Vlok, Wynand. “Aspekte van die spermatologie van die kleinbekgeelvis, Barbus aeneus (Cyprinidae).” 2014. Thesis, University of Johannesburg. Accessed April 03, 2020. http://hdl.handle.net/10210/9674.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Vlok, Wynand. “Aspekte van die spermatologie van die kleinbekgeelvis, Barbus aeneus (Cyprinidae).” 2014. Web. 03 Apr 2020.

Vancouver:

Vlok W. Aspekte van die spermatologie van die kleinbekgeelvis, Barbus aeneus (Cyprinidae). [Internet] [Thesis]. University of Johannesburg; 2014. [cited 2020 Apr 03]. Available from: http://hdl.handle.net/10210/9674.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Vlok W. Aspekte van die spermatologie van die kleinbekgeelvis, Barbus aeneus (Cyprinidae). [Thesis]. University of Johannesburg; 2014. Available from: http://hdl.handle.net/10210/9674

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidad de Extremadura

23. Miró Morán, Álvaro. Identificación, regulación y estudio funcional de la proteína quinasa N-terminal de c-Jun (JNK) en espermatozoides de mamíferos .

Degree: 2013, Universidad de Extremadura

URL: http://hdl.handle.net/10662/521

► En este trabajo de investigación demostramos por primera vez la presencia y la localización subcelular de la quinasa N-terminal de c-Jun (JNK), en lisados de… (more)

Subjects/Keywords: Espermatozoides; JNK; Viabilidad; Spermatozoa; Viability

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APA (6^th Edition):

Miró Morán, �. (2013). Identificación, regulación y estudio funcional de la proteína quinasa N-terminal de c-Jun (JNK) en espermatozoides de mamíferos . (Thesis). Universidad de Extremadura. Retrieved from http://hdl.handle.net/10662/521

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Miró Morán, Álvaro. “Identificación, regulación y estudio funcional de la proteína quinasa N-terminal de c-Jun (JNK) en espermatozoides de mamíferos .” 2013. Thesis, Universidad de Extremadura. Accessed April 03, 2020. http://hdl.handle.net/10662/521.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Miró Morán, Álvaro. “Identificación, regulación y estudio funcional de la proteína quinasa N-terminal de c-Jun (JNK) en espermatozoides de mamíferos .” 2013. Web. 03 Apr 2020.

Vancouver:

Miró Morán �. Identificación, regulación y estudio funcional de la proteína quinasa N-terminal de c-Jun (JNK) en espermatozoides de mamíferos . [Internet] [Thesis]. Universidad de Extremadura; 2013. [cited 2020 Apr 03]. Available from: http://hdl.handle.net/10662/521.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Miró Morán �. Identificación, regulación y estudio funcional de la proteína quinasa N-terminal de c-Jun (JNK) en espermatozoides de mamíferos . [Thesis]. Universidad de Extremadura; 2013. Available from: http://hdl.handle.net/10662/521

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of British Columbia

24. Bols, Niels Christian. Studies on sperm histones in amphibia and chondrichthyes .

Degree: 1972, University of British Columbia

URL: http://hdl.handle.net/2429/33467

► The basic protein composition of sperm, as well as the change in basic proteins during spermiogenesis, has been studied in a number of organisms, using… (more)

Subjects/Keywords: Spermatozoa; Histones

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bols, N. C. (1972). Studies on sperm histones in amphibia and chondrichthyes . (Thesis). University of British Columbia. Retrieved from http://hdl.handle.net/2429/33467

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Bols, Niels Christian. “Studies on sperm histones in amphibia and chondrichthyes .” 1972. Thesis, University of British Columbia. Accessed April 03, 2020. http://hdl.handle.net/2429/33467.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Bols, Niels Christian. “Studies on sperm histones in amphibia and chondrichthyes .” 1972. Web. 03 Apr 2020.

Vancouver:

Bols NC. Studies on sperm histones in amphibia and chondrichthyes . [Internet] [Thesis]. University of British Columbia; 1972. [cited 2020 Apr 03]. Available from: http://hdl.handle.net/2429/33467.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Bols NC. Studies on sperm histones in amphibia and chondrichthyes . [Thesis]. University of British Columbia; 1972. Available from: http://hdl.handle.net/2429/33467

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Newcastle

25. Samarasinghe Vidane Arachchige, Chamila. Toxicity of parabens and metal oxide nanoparticles (TiO₂ and ZnO) to human spermatozoa and earthworm reproduction.

Degree: PhD, 2019, University of Newcastle

URL: http://hdl.handle.net/1959.13/1407519

►

Research Doctorate - Doctor of Philosophy (PhD)

Parabens and nanoparticles are used as additives in most of the consumer products. Increased use of the consumer… (more)

▼

Research Doctorate - Doctor of Philosophy (PhD)

Parabens and nanoparticles are used as additives in most of the consumer products. Increased use of the consumer products containing these compounds and their continuous release to the environment may lead to negative consequences in humans and organisms if these compounds possess any toxic potential. Parabens are commonly used in personal care products as a preservative. Metal oxide nanoparticles are also used in commercial products. The commonality between parabens, TiO₂ nanoparticles and ZnO nanoparticles is all these compounds are common additives in sunscreens, cosmetics, pharmaceuticals and foods. The endocrine disrupting effects of parabens and various characteristics of nanoparticles such as higher reactivity, ability to penetrate biological barriers (Blood-brain barrier, testis barrier) have risen global concerns on their environmental and public health hazards. Toxicity investigations using earthworms is a frequently used biomarker in terrestrial ecotoxicity assessments. There are many different measures for public health hazard assessments. Male reproductive health is one of the sensitive measures that can be used to predict the toxic effects of compounds on humans. To gain insight about the public health hazard of these compounds, the oxidative potential of parabens (methyl, propyl, and butylparaben) and nanoparticles TiO₂ anatase, rutile, and anatase/rutile mixture) to human spermatozoa was investigated. Then, the ecological hazard of parabens and metal oxide nanoparticles on earthworms were studied. In this study, acute and chronic toxicity of these compounds to adult earthworms was assessed. Nanoparticles, being a persistent and emerging pollutant in the terrestrial environment, were tested for their long term effects on lifecycle traits of juvenile earthworms. Parabens showed their potential to generate oxidative stress in human spermatozoa at the concentrations relevant to human use (Eg. methylparaben, at 13 mM, a concentration normally used as vaginal lubricants). None of the crystal types of TiO₂ nanoparticles used in this study showed oxidative stress against human spermatozoa at the environmentally relevant concentrations. Parabens were readily degraded in soil and did not cause acute or chronic toxicity to earthworms. Two soils with different characteristics were used in the nanoparticle toxicity assays. Upon a comparatively shorter exposure duration (28 days), both TiO₂ nanoparticles and ZnO nanoparticles did not show significant deleterious effects on the survival and reproductive outcome of adult earthworms. The exception was at high concentrations (500 and 1000 mg Kg⁻¹), where ZnO showed reproductive toxicity only in one soil. The toxicity mechanism was not clear. The juveniles born to these parent earthworms (F1 generation) were further exposed to TiO₂ anatase, TiO₂ rutile and ZnO nanoparticles (0.1 – 1000 mg Kg⁻¹) in two soils (same exposure, similar soil). The survival, growth, sexual maturity and the reproduction of these F1 individuals…

Advisors/Committee Members: University of Newcastle. Faculty of Science, Global Centre for Environmental Remediation.

Subjects/Keywords: nanoparticles; parabens; toxicity; earthworm; spermatozoa

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Samarasinghe Vidane Arachchige, C. (2019). Toxicity of parabens and metal oxide nanoparticles (TiO₂ and ZnO) to human spermatozoa and earthworm reproduction. (Doctoral Dissertation). University of Newcastle. Retrieved from http://hdl.handle.net/1959.13/1407519

Chicago Manual of Style (16^th Edition):

Samarasinghe Vidane Arachchige, Chamila. “Toxicity of parabens and metal oxide nanoparticles (TiO₂ and ZnO) to human spermatozoa and earthworm reproduction.” 2019. Doctoral Dissertation, University of Newcastle. Accessed April 03, 2020. http://hdl.handle.net/1959.13/1407519.

MLA Handbook (7^th Edition):

Samarasinghe Vidane Arachchige, Chamila. “Toxicity of parabens and metal oxide nanoparticles (TiO₂ and ZnO) to human spermatozoa and earthworm reproduction.” 2019. Web. 03 Apr 2020.

Vancouver:

Samarasinghe Vidane Arachchige C. Toxicity of parabens and metal oxide nanoparticles (TiO₂ and ZnO) to human spermatozoa and earthworm reproduction. [Internet] [Doctoral dissertation]. University of Newcastle; 2019. [cited 2020 Apr 03]. Available from: http://hdl.handle.net/1959.13/1407519.

Council of Science Editors:

Samarasinghe Vidane Arachchige C. Toxicity of parabens and metal oxide nanoparticles (TiO₂ and ZnO) to human spermatozoa and earthworm reproduction. [Doctoral Dissertation]. University of Newcastle; 2019. Available from: http://hdl.handle.net/1959.13/1407519

Drexel University

26. Urbano, Leonardo F. Robust Automatic Multi-Sperm Tracking in Time-Lapse Images.

Degree: 2014, Drexel University

URL: http://hdl.handle.net/1860/4575

► Human sperm cell counting, tracking and motility analysis is of significant interest to biologists studying sperm function and to medical practitioners evaluating male infertility. Today,… (more)

▼ Human sperm cell counting, tracking and motility analysis is of significant interest to biologists studying sperm function and to medical practitioners evaluating male infertility. Today, the prevailing method for analyzing sperm at fertility clinics and research laboratories is laborious and subjective. Namely, the number and quality of sperm are often visually appraised by technicians using a microscope. Although total sperm count and sperm concentration can be reasonably estimated when standard protocols are applied, they have little diagnostic value except in identifying pathologically extreme abnormalities. More dynamic sperm swimming parameters such as curvilinear velocity (VCL), straight-line velocity (VSL), linearity of forward progression (LIN) and amplitude of lateral head displacement (ALH) are increasingly believed to have clinical significance in predicting infertility but are impossible for a human observer to visually discern. Expensive computer-assisted semen analysis (CASA) instruments are also sometimes used but are severely encumbered by crude ad-hoc tracking algorithms which cannot track sperm in close proximity or whose paths intersect and are typically limited to analyzing video clips of < 1 sec duration.In this thesis, we present a robust automatic multi-sperm tracking algorithm that can measure dynamic sperm motility parameters over time in pre-recorded time-lapse images. This effort is informed by progress in signal processing and target tracking technologies over the last three decades. Multi-target tracking algorithms originally developed for radar, sonar and video processing have addressed similar problems in other domains. In this thesis, we demonstrate that their methodologies can be used for sperm tracking and motility analysis. To resolve sperm measurement-to-track association conflicts, we applied and evaluated three multi-target tracking algorithms: the probabilistic data association filter (PDAF), the joint probabilistic data association filter (JPDAF) and the exact nearest neighbor extension to the JPDAF (ENN-JPDAF). We validated the accuracy of our tracking and motility analysis by using simulated sperm trajectories whose ground truth tracks were perfectly known. Using samples collected from five patients at a fertility clinic, we demonstrated automatic sperm detection and tracking even during challenging multi-sperm collision events. Combined analysis, testing and simulation support the use of probabilistic data association techniques robust automatic multi-sperm tracking. This method could provide fertility specialists with new data visualizations and interpretations previously impossible with existing laboratory protocols. Advisors/Committee Members: Kam, Moshe.

Subjects/Keywords: Electrical engineering; Spermatozoa; Fertilization (Biology)

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Urbano, L. F. (2014). Robust Automatic Multi-Sperm Tracking in Time-Lapse Images. (Thesis). Drexel University. Retrieved from http://hdl.handle.net/1860/4575

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Urbano, Leonardo F. “Robust Automatic Multi-Sperm Tracking in Time-Lapse Images.” 2014. Thesis, Drexel University. Accessed April 03, 2020. http://hdl.handle.net/1860/4575.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Urbano, Leonardo F. “Robust Automatic Multi-Sperm Tracking in Time-Lapse Images.” 2014. Web. 03 Apr 2020.

Vancouver:

Urbano LF. Robust Automatic Multi-Sperm Tracking in Time-Lapse Images. [Internet] [Thesis]. Drexel University; 2014. [cited 2020 Apr 03]. Available from: http://hdl.handle.net/1860/4575.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Urbano LF. Robust Automatic Multi-Sperm Tracking in Time-Lapse Images. [Thesis]. Drexel University; 2014. Available from: http://hdl.handle.net/1860/4575

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Michigan State University

27. Shaffner, Clyde Samuel. Viability of spermatozoa of chickens under various environmental conditions.

Degree: MS, 1940, Michigan State University

URL: http://etd.lib.msu.edu/islandora/object/etd:12278

Subjects/Keywords: Poultry; Spermatozoa

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Shaffner, C. S. (1940). Viability of spermatozoa of chickens under various environmental conditions. (Masters Thesis). Michigan State University. Retrieved from http://etd.lib.msu.edu/islandora/object/etd:12278

Chicago Manual of Style (16^th Edition):

Shaffner, Clyde Samuel. “Viability of spermatozoa of chickens under various environmental conditions.” 1940. Masters Thesis, Michigan State University. Accessed April 03, 2020. http://etd.lib.msu.edu/islandora/object/etd:12278.

MLA Handbook (7^th Edition):

Shaffner, Clyde Samuel. “Viability of spermatozoa of chickens under various environmental conditions.” 1940. Web. 03 Apr 2020.

Vancouver:

Shaffner CS. Viability of spermatozoa of chickens under various environmental conditions. [Internet] [Masters thesis]. Michigan State University; 1940. [cited 2020 Apr 03]. Available from: http://etd.lib.msu.edu/islandora/object/etd:12278.

Council of Science Editors:

Shaffner CS. Viability of spermatozoa of chickens under various environmental conditions. [Masters Thesis]. Michigan State University; 1940. Available from: http://etd.lib.msu.edu/islandora/object/etd:12278

University of Pretoria

28. Heise, Annett. Influence of seminal plasma on fresh and post-thaw parameters of stallion epididymal spermatozoa.

Degree: Production Animal Studies, 2010, University of Pretoria

URL: http://hdl.handle.net/2263/30504

► Cryopreservation of epididymal spermatozoa may be the only opportunity to preserve valuable genetics of males in cases of unforeseen injury or death. Stallion epididymal spermatozoa… (more)

▼ Cryopreservation of epididymal spermatozoa may be the only opportunity to preserve valuable genetics of males in cases of unforeseen injury or death. Stallion epididymal spermatozoa have been cryopreserved successfully and it has been demonstrated that stallion epididymal spermatozoa are fertile, and pregnancies as well as live foals have been produced. As spermatozoal quality parameters like motility, morphology and viability have a major influence on fertility and pregnancy rates, it is of great interest to describe these and investigate the influence of seminal plasma on these parameters. Fresh and post-thaw parameters (motility, morphology and viability) of stallion epididymal spermatozoa that have been and that have not been exposed to seminal plasma were evaluated, and directly compared to fresh and post-thaw parameters of ejaculated spermatozoa from the same stallions. Six sperm categories of each stallion (n= 4) were evaluated for motility, morphology and viability. These categories were fresh ejaculated spermatozoa (Fr-E), fresh epididymal spermatozoa that had been exposed to seminal plasma (Fr-SP+), fresh epididymal spermatozoa that had never been exposed to seminal plasma (Fr-SP-), frozen-thawed ejaculated spermatozoa (Cr-E), frozen-thawed epididymal spermatozoa that had been exposed to seminal plasma prior to freezing (Cr-SP+) and frozen-thawed epididymal spermatozoa that had never been exposed to seminal plasma (Cr-SP-). Results show that seminal plasma stimulates initial motility of fresh epididymal stallion spermatozoa while this difference in progressive motility is no longer present post-thaw; and that progressive motility of fresh or frozen-thawed ejaculated stallion spermatozoa is not always a good indicator for post-thaw progressive motility of epididymal spermatozoa. This study shows that seminal plasma has a positive influence on the incidence of overall sperm defects, midpiece reflexes and distal cytoplasmic droplets in frozen-thawed stallion epididymal spermatozoa while the occurance of midpiece reflexes is likely to be linked to distal cytoplasmic droplets. Furthermore, we could show that seminal plasma does not have an influence on viability of fresh and frozen-thawed morphologically normal epididymal spermatozoa. In conclusion, we recommend the retrograde flushing technique using seminal plasma as flushing medium to harvest and freeze stallion epididymal spermatozoa. AFRIKAANS : Bevriesing van epididimale spermatozoa mag die enigste geleentheid wees om waardevolle genetika van manlike diere te bewaar in die geval van onverwagse dood of besering. Epididimale spermatozoa van hingste is al suksesvol bevries, is bewys vrugbaar te wees en dragtighede sowel as lewendige vullens is deur die gebruik daarvan gelewer. Aangesien maatstawwe van spermgehalte soos beweeglikheid, morfologie en lewensvatbaarheid ‘n beduidende invloed uitoefen op vrugbaarheid en dragtigheidsyfers, is dit van groot belang om hierdie maatstawwe te omskryf en die invloed van seminale plasma daarop te ondersoek. Vars en… Advisors/Committee Members: Prof D Gerber (advisor).

Subjects/Keywords: Epididimale spermatozoa mag; Stallions; Post-thaw parameters; UCTD; Epididymal spermatozoa

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Heise, A. (2010). Influence of seminal plasma on fresh and post-thaw parameters of stallion epididymal spermatozoa. (Masters Thesis). University of Pretoria. Retrieved from http://hdl.handle.net/2263/30504

Chicago Manual of Style (16^th Edition):

Heise, Annett. “Influence of seminal plasma on fresh and post-thaw parameters of stallion epididymal spermatozoa.” 2010. Masters Thesis, University of Pretoria. Accessed April 03, 2020. http://hdl.handle.net/2263/30504.

MLA Handbook (7^th Edition):

Heise, Annett. “Influence of seminal plasma on fresh and post-thaw parameters of stallion epididymal spermatozoa.” 2010. Web. 03 Apr 2020.

Vancouver:

Heise A. Influence of seminal plasma on fresh and post-thaw parameters of stallion epididymal spermatozoa. [Internet] [Masters thesis]. University of Pretoria; 2010. [cited 2020 Apr 03]. Available from: http://hdl.handle.net/2263/30504.

Council of Science Editors:

Heise A. Influence of seminal plasma on fresh and post-thaw parameters of stallion epididymal spermatozoa. [Masters Thesis]. University of Pretoria; 2010. Available from: http://hdl.handle.net/2263/30504

University of Pretoria

29. [No author]. Influence of seminal plasma on fresh and post-thaw parameters of stallion epididymal spermatozoa .

Degree: 2010, University of Pretoria

URL: http://upetd.up.ac.za/thesis/available/etd-12212010-164755/

► Cryopreservation of epididymal spermatozoa may be the only opportunity to preserve valuable genetics of males in cases of unforeseen injury or death. Stallion epididymal spermatozoa… (more)

▼ Cryopreservation of epididymal spermatozoa may be the only opportunity to preserve valuable genetics of males in cases of unforeseen injury or death. Stallion epididymal spermatozoa have been cryopreserved successfully and it has been demonstrated that stallion epididymal spermatozoa are fertile, and pregnancies as well as live foals have been produced. As spermatozoal quality parameters like motility, morphology and viability have a major influence on fertility and pregnancy rates, it is of great interest to describe these and investigate the influence of seminal plasma on these parameters. Fresh and post-thaw parameters (motility, morphology and viability) of stallion epididymal spermatozoa that have been and that have not been exposed to seminal plasma were evaluated, and directly compared to fresh and post-thaw parameters of ejaculated spermatozoa from the same stallions. Six sperm categories of each stallion (n= 4) were evaluated for motility, morphology and viability. These categories were fresh ejaculated spermatozoa (Fr-E), fresh epididymal spermatozoa that had been exposed to seminal plasma (Fr-SP+), fresh epididymal spermatozoa that had never been exposed to seminal plasma (Fr-SP-), frozen-thawed ejaculated spermatozoa (Cr-E), frozen-thawed epididymal spermatozoa that had been exposed to seminal plasma prior to freezing (Cr-SP+) and frozen-thawed epididymal spermatozoa that had never been exposed to seminal plasma (Cr-SP-). Results show that seminal plasma stimulates initial motility of fresh epididymal stallion spermatozoa while this difference in progressive motility is no longer present post-thaw; and that progressive motility of fresh or frozen-thawed ejaculated stallion spermatozoa is not always a good indicator for post-thaw progressive motility of epididymal spermatozoa. This study shows that seminal plasma has a positive influence on the incidence of overall sperm defects, midpiece reflexes and distal cytoplasmic droplets in frozen-thawed stallion epididymal spermatozoa while the occurance of midpiece reflexes is likely to be linked to distal cytoplasmic droplets. Furthermore, we could show that seminal plasma does not have an influence on viability of fresh and frozen-thawed morphologically normal epididymal spermatozoa. In conclusion, we recommend the retrograde flushing technique using seminal plasma as flushing medium to harvest and freeze stallion epididymal spermatozoa. AFRIKAANS : Bevriesing van epididimale spermatozoa mag die enigste geleentheid wees om waardevolle genetika van manlike diere te bewaar in die geval van onverwagse dood of besering. Epididimale spermatozoa van hingste is al suksesvol bevries, is bewys vrugbaar te wees en dragtighede sowel as lewendige vullens is deur die gebruik daarvan gelewer. Aangesien maatstawwe van spermgehalte soos beweeglikheid, morfologie en lewensvatbaarheid ‘n beduidende invloed uitoefen op vrugbaarheid en dragtigheidsyfers, is dit van groot belang om hierdie maatstawwe te omskryf en die invloed van seminale plasma daarop te ondersoek. Vars en… Advisors/Committee Members: Prof D Gerber (advisor).

Subjects/Keywords: Epididimale spermatozoa mag; Stallions; Post-thaw parameters; UCTD; Epididymal spermatozoa

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

author], [. (2010). Influence of seminal plasma on fresh and post-thaw parameters of stallion epididymal spermatozoa . (Masters Thesis). University of Pretoria. Retrieved from http://upetd.up.ac.za/thesis/available/etd-12212010-164755/

Chicago Manual of Style (16^th Edition):

author], [No. “Influence of seminal plasma on fresh and post-thaw parameters of stallion epididymal spermatozoa .” 2010. Masters Thesis, University of Pretoria. Accessed April 03, 2020. http://upetd.up.ac.za/thesis/available/etd-12212010-164755/.

MLA Handbook (7^th Edition):

author], [No. “Influence of seminal plasma on fresh and post-thaw parameters of stallion epididymal spermatozoa .” 2010. Web. 03 Apr 2020.

Vancouver:

author] [. Influence of seminal plasma on fresh and post-thaw parameters of stallion epididymal spermatozoa . [Internet] [Masters thesis]. University of Pretoria; 2010. [cited 2020 Apr 03]. Available from: http://upetd.up.ac.za/thesis/available/etd-12212010-164755/.

Council of Science Editors:

author] [. Influence of seminal plasma on fresh and post-thaw parameters of stallion epididymal spermatozoa . [Masters Thesis]. University of Pretoria; 2010. Available from: http://upetd.up.ac.za/thesis/available/etd-12212010-164755/

University of New South Wales

30. Vermey, Belinda. Can polarised light microscopy (PLM) be used as a non-invasive tool for selecting the optimal sperm for ICSI?.

Degree: Women's & Children's Health, 2013, University of New South Wales

URL: http://handle.unsw.edu.au/1959.4/53015 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:11693/SOURCE01?view=true

► Aim:To investigate PLM as a reproducible non-invasive method in the assessment of sperm head retardance, and observing PLM within a clinical setting to select an… (more)

▼ Aim:To investigate PLM as a reproducible non-invasive method in the assessment of sperm head retardance, and observing PLM within a clinical setting to select an optimal sperm for ICSI.Methods:1. PLM (OosightTM) images from 368 spermatozoa were assessed to establish intra and inter-observer variation.2. A range and frequency of head retardance was developed from pooled samples and then analysed further comparing normal and abnormal head morphology.3. Normal sperm selected for ICSI according to the W.H.O 5th edition, were imaged prior to injection. Images were analysed later-blinded to the ICSI outcome. Single embryo culture and elective single blastocyst transfer (eSET) were undertaken. PLM retardance was compared with embryo fertilisation, cleavage, blastulation, utilisation and clinical pregnancy rates using independent sample T tests, Pearson's correlation coefficients, ROC curves and X2 tests.Results:1. The intra/inter-observer variation revealed our method to be reproducible by an Intra-class correlation coefficient and Pearson's co-efficient P<0.05.2. The pooled sperm sample had mean retardance 0.97 +/-0.27nm, range 0.49-1.89nm. When comparing the range with head morphology it was found that retardance significantly decreases with normality (P<0.05).3. 63 fresh and 38 frozen cases were analysed. 486 oocytes were injected and 364 fertilised (74.9%). 101 cumulative fresh and subsequent frozen single blastocysts were transferred (eSET) to produce 34 clinical pregnancies, 33.7% per Tx, 54.0% per case (fresh and frozen per OPU). There were no significant differences (P>0.05) in sperm retardance when comparing normally fertilised oocytes against either abnormal/nil fertilisation. Good quality embryos had lower retardance (P<0.05) where ≤0.91nm was optimum. There were no significant differences (P>0.05) in retardance when comparing blastulation and failed blastulation, however utilised embryos had lower retardance compared to discarded embryos (P<0.05). Embryos with lower retardance were more successful (P<0.05) at clinical pregnancies where ≤0.91nm was optimum.Conclusion:PLM head retardance is a reproducible non-invasive method of assessing sperm. Our results suggest sperm with head retardance ≤0.91nm are more likely to result in a clinical pregnancy. In a prospective manner, selecting sperm with lower sperm retardance may result in more embryos with good quality in culture and higher clinical pregnancy rates. Advisors/Committee Members: Chapman, Michael, Women's & Children's Health, Faculty of Medicine, UNSW, Cooke, Simon, Women's & Children's Health, Faculty of Medicine, UNSW.

Subjects/Keywords: Selecting optimal spermatozoa; Optimal human spermatozoa; Polarised light microscopy; Clinical pregnancies

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Vermey, B. (2013). Can polarised light microscopy (PLM) be used as a non-invasive tool for selecting the optimal sperm for ICSI?. (Masters Thesis). University of New South Wales. Retrieved from http://handle.unsw.edu.au/1959.4/53015 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:11693/SOURCE01?view=true

Chicago Manual of Style (16^th Edition):

Vermey, Belinda. “Can polarised light microscopy (PLM) be used as a non-invasive tool for selecting the optimal sperm for ICSI?.” 2013. Masters Thesis, University of New South Wales. Accessed April 03, 2020. http://handle.unsw.edu.au/1959.4/53015 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:11693/SOURCE01?view=true.

MLA Handbook (7^th Edition):

Vermey, Belinda. “Can polarised light microscopy (PLM) be used as a non-invasive tool for selecting the optimal sperm for ICSI?.” 2013. Web. 03 Apr 2020.

Vancouver:

Vermey B. Can polarised light microscopy (PLM) be used as a non-invasive tool for selecting the optimal sperm for ICSI?. [Internet] [Masters thesis]. University of New South Wales; 2013. [cited 2020 Apr 03]. Available from: http://handle.unsw.edu.au/1959.4/53015 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:11693/SOURCE01?view=true.

Council of Science Editors:

Vermey B. Can polarised light microscopy (PLM) be used as a non-invasive tool for selecting the optimal sperm for ICSI?. [Masters Thesis]. University of New South Wales; 2013. Available from: http://handle.unsw.edu.au/1959.4/53015 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:11693/SOURCE01?view=true

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Open Access Theses and Dissertations