subject:(Solid Phase Peptide Synthesis)

University of Adelaide

1. Horsfall, Aimee Jade. The synthesis of bimane constrained peptides and their fluorescent and structural properties.

Degree: 2017, University of Adelaide

URL: http://hdl.handle.net/2440/104675

► Aberrant protein-protein interactions often result in disease, and as such, effective protein-protein interaction inhibitors are needed to mitigate the disease state. These interaction interfaces often… (more)

▼ Aberrant protein-protein interactions often result in disease, and as such, effective protein-protein interaction inhibitors are needed to mitigate the disease state. These interaction interfaces often involve secondary structural motifs, for example, an α-helix or β-sheet. Small molecule drugs are not well suited to inhibit protein-protein interactions however constrained peptides, have shown to have great therapeutic potential. Short peptides display little secondary structure in aqueous solution and as such, peptide sequences derived from a protein-protein interaction interface for use as a protein-protein interaction inhibitor, must be constrained into the native secondary structure. This can be achieved by installing a linker between the side-chains of two appropriately spaced amino-acids in the sequence. Many different linker chemistries have been designed and implemented with good biological results. However, these constrained peptide therapeutics are still restricted by traditional small molecule drug hurdles including cell permeability, protease degradation and the ability to visualise and track a molecule intracellularly. Linkers such as the all-hydrocarbon metathesis linker have shown great promise in reducing protease degradation and increasing cell permeability, however a fluorescent tag is still necessary to visualise a drug candidate. Here, a bimane linker is proposed as a new peptide linker to help overcome these limitations. Dibromobimane is reacted with thiol-containing amino-acid side chains to introduce a new fluorescent constraint in a series of model peptides. The reaction conditions with dibromobimane are optimised in solution to reveal that a buffered system is required for the cyclisation to occur efficiently. Optimal reaction conditions, determined by monitoring the increase of the fluorescent product, were 0.5 mg/ml peptide in 10 mM PBS with one equivalent of dibromobimane. The reaction was shown to be facile and versatile; in this thesis an array of peptides with varied sequence length, constraint length and amino-acid composition were cyclised under the same conditions, all reaching reaction completion in under 30 minutes. Additionally, these same conditions were applied successfully to react monobromobimane with series of short peptides. Cyclisation on reaction with dibromobimane, was also demonstrated on-resin with similar efficiency. The fluorescent properties of the resultant peptides were then explored to reveal that pH does not affect the observed fluorescence however a longer peptide length resulted in greater fluorescence intensity. Furthermore, acyclic mono-bimane-functionalised peptides displayed lower fluorescence intensity than the bimane-cyclised counterparts. The fluorescence of the bimane cyclised peptide could be detected as low as 10 nM on a plate reader, which is expected to further improve on a more sensitive instrument. The secondary structure of a series of tri- and penta-peptides were investigated through CD and NMR techniques. It was deduced that the bimane linker… Advisors/Committee Members: Abell, Andrew David (advisor), Heng, Sabrina (advisor), School of Physical Sciences (school).

Subjects/Keywords: bromobimane; peptide; constrained peptides; fluorescence; secondary structure; solid-phase peptide synthesis

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APA (6^th Edition):

Horsfall, A. J. (2017). The synthesis of bimane constrained peptides and their fluorescent and structural properties. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/104675

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Horsfall, Aimee Jade. “The synthesis of bimane constrained peptides and their fluorescent and structural properties.” 2017. Thesis, University of Adelaide. Accessed January 18, 2021. http://hdl.handle.net/2440/104675.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Horsfall, Aimee Jade. “The synthesis of bimane constrained peptides and their fluorescent and structural properties.” 2017. Web. 18 Jan 2021.

Vancouver:

Horsfall AJ. The synthesis of bimane constrained peptides and their fluorescent and structural properties. [Internet] [Thesis]. University of Adelaide; 2017. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/2440/104675.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Horsfall AJ. The synthesis of bimane constrained peptides and their fluorescent and structural properties. [Thesis]. University of Adelaide; 2017. Available from: http://hdl.handle.net/2440/104675

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Sydney

2. Hsieh, Yves Shang-Yi. Chemical Synthesis of Peptides & Proteins with Post-Translational Modifications .

Degree: 2013, University of Sydney

URL: http://hdl.handle.net/2123/9422

► This thesis describes efforts aimed at the synthesis of peptides and proteins bearing homogeneous carbohydrate and sulfate modifications for interrogation of the structural and functional… (more)

Subjects/Keywords: protein; peptide; post-translational modifications; glycosylation; sulfation; solid-phase peptide synthesis

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APA (6^th Edition):

Hsieh, Y. S. (2013). Chemical Synthesis of Peptides & Proteins with Post-Translational Modifications . (Thesis). University of Sydney. Retrieved from http://hdl.handle.net/2123/9422

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Hsieh, Yves Shang-Yi. “Chemical Synthesis of Peptides & Proteins with Post-Translational Modifications .” 2013. Thesis, University of Sydney. Accessed January 18, 2021. http://hdl.handle.net/2123/9422.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Hsieh, Yves Shang-Yi. “Chemical Synthesis of Peptides & Proteins with Post-Translational Modifications .” 2013. Web. 18 Jan 2021.

Vancouver:

Hsieh YS. Chemical Synthesis of Peptides & Proteins with Post-Translational Modifications . [Internet] [Thesis]. University of Sydney; 2013. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/2123/9422.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hsieh YS. Chemical Synthesis of Peptides & Proteins with Post-Translational Modifications . [Thesis]. University of Sydney; 2013. Available from: http://hdl.handle.net/2123/9422

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Minnesota

3. Diaz-Rodriguez, Veronica. Solid-Phase Synthesis Of Prenylated Peptides Containing C-Terminal Esters: A Chemical Biology Tool For The Study Of Protein Prenylation.

Degree: PhD, Chemistry, 2015, University of Minnesota

URL: http://hdl.handle.net/11299/183386

► Protein prenylation is a post-translational modification that comprises the attachment of either a farnesyl or a geranylgeranyl isoprenoid. This covalent, irreversible modification has been studied… (more)

Subjects/Keywords: a-factor; cysteine; peptides; prenylation; racemization; solid phase peptide synthesis

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APA (6^th Edition):

Diaz-Rodriguez, V. (2015). Solid-Phase Synthesis Of Prenylated Peptides Containing C-Terminal Esters: A Chemical Biology Tool For The Study Of Protein Prenylation. (Doctoral Dissertation). University of Minnesota. Retrieved from http://hdl.handle.net/11299/183386

Chicago Manual of Style (16^th Edition):

Diaz-Rodriguez, Veronica. “Solid-Phase Synthesis Of Prenylated Peptides Containing C-Terminal Esters: A Chemical Biology Tool For The Study Of Protein Prenylation.” 2015. Doctoral Dissertation, University of Minnesota. Accessed January 18, 2021. http://hdl.handle.net/11299/183386.

MLA Handbook (7^th Edition):

Diaz-Rodriguez, Veronica. “Solid-Phase Synthesis Of Prenylated Peptides Containing C-Terminal Esters: A Chemical Biology Tool For The Study Of Protein Prenylation.” 2015. Web. 18 Jan 2021.

Vancouver:

Diaz-Rodriguez V. Solid-Phase Synthesis Of Prenylated Peptides Containing C-Terminal Esters: A Chemical Biology Tool For The Study Of Protein Prenylation. [Internet] [Doctoral dissertation]. University of Minnesota; 2015. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/11299/183386.

Council of Science Editors:

Diaz-Rodriguez V. Solid-Phase Synthesis Of Prenylated Peptides Containing C-Terminal Esters: A Chemical Biology Tool For The Study Of Protein Prenylation. [Doctoral Dissertation]. University of Minnesota; 2015. Available from: http://hdl.handle.net/11299/183386

NSYSU

4. Cheng, Kai-wen. Synthesis of Cyclic Peptide-Based Grb2 âSH2 Inhibitor via Ring-Closing Metathesis in Water.

Degree: Master, Chemistry, 2016, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0701116-170035

► Cyclic peptides have gradually became a new type of therapeutic agent in medicine. They have been useful in treatment of bacCyclic peptides have gradually became… (more)

Subjects/Keywords: ring-closing metathesi; Solid-phase peptide synthesis; Ras protein; Cyclic peptide; Grb2-SH2

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APA (6^th Edition):

Cheng, K. (2016). Synthesis of Cyclic Peptide-Based Grb2 âSH2 Inhibitor via Ring-Closing Metathesis in Water. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0701116-170035

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Cheng, Kai-wen. “Synthesis of Cyclic Peptide-Based Grb2 âSH2 Inhibitor via Ring-Closing Metathesis in Water.” 2016. Thesis, NSYSU. Accessed January 18, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0701116-170035.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Cheng, Kai-wen. “Synthesis of Cyclic Peptide-Based Grb2 âSH2 Inhibitor via Ring-Closing Metathesis in Water.” 2016. Web. 18 Jan 2021.

Vancouver:

Cheng K. Synthesis of Cyclic Peptide-Based Grb2 âSH2 Inhibitor via Ring-Closing Metathesis in Water. [Internet] [Thesis]. NSYSU; 2016. [cited 2021 Jan 18]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0701116-170035.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Cheng K. Synthesis of Cyclic Peptide-Based Grb2 âSH2 Inhibitor via Ring-Closing Metathesis in Water. [Thesis]. NSYSU; 2016. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0701116-170035

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Auckland

5. Kowalczyk, Renata. Synthesis of mannosylated peptides as components for synthetic vaccines.

Degree: 2008, University of Auckland

URL: http://hdl.handle.net/2292/3341

► The immune system often recognises tumour cells and infectious agents from the unique peptides found on their surfaces therefore, synthetic peptides of similar structure can… (more)

▼ The immune system often recognises tumour cells and infectious agents from the unique peptides found on their surfaces therefore, synthetic peptides of similar structure can be used as vaccines to stimulate the immune system. Despite the problems associated with proteolysis and delivery to the immune system, peptide-based vaccines have enormous potential due to their ease of synthesis and purification. The aim of this research was to synthesise ligands for mannose receptors (MRs) that are found on human Antigen Presenting Cells (APCs), for use in synthetic vaccines. Carbohydrate bearing antigens are recognised by MRs which play an important role in binding antigens, migration of dendritic cells (DCs) and interaction of DCs with lymphocytes. Hence, incorporation of a sugar residue into a peptide chain can be used to enhance antigen presentation. This thesis describes the synthesis of fluorescein labelled O-mannosylated peptides using either manual or microwave assisted solid phase glycopeptide synthesis (SPGS) on pre-loaded WANG resin. The mannosylated peptides thus prepared can be tested for their ability to bind mannose receptors on human APCs in vitro. In order to prepare compounds that could be analysed in biological screens, a fluorescent label (5(6)-carboxyfluorescein) was introduced into the glycopeptides via the N??- or the N??-amino group of the lysine residue. It was found that preparation of the glycopeptide was more facile when the peptide chain was built onto the N?? of Lys (label into N??) rather than onto the N?? of Lys (label into N??). In order to overcome problems experienced when introducing more than one glycosylated building block into the peptide chain, a polyethylene glycol (PEG) linker was employed as a sugar carrier. It was found that mono- and dimannosylated building blocks attached to PEG carrier were incorporated more easily into the peptide chain compared to mono- and dimannosylated serine units. Importantly, microwave technology (CEM Liberty microwave peptide synthesiser) was used for SPGS which resulted in improved purity and yields of the glycopeptides thus prepared with a significant reduction in reaction times. The first fifteen glycopeptides prepared in the present study were tested for binding to mannose receptors. Several compounds have shown improved binding to monocytes (bear MRs) in comparison to lymphocytes (do not bear MRs), in the presence of calcium ions. Calcium dependent binding is specific for C type lectin receptor family that MRs belong to. Five remaining glycopeptides are currently undergoing biological evaluation. Advisors/Committee Members: Prof Margaret A. Brimble, Dr Paul W.R. Harris.

Subjects/Keywords: glycopeptides; solid phase peptide synthesis; glycosylation; mannose; peptide vaccines; microwave; mannose receptor

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kowalczyk, R. (2008). Synthesis of mannosylated peptides as components for synthetic vaccines. (Doctoral Dissertation). University of Auckland. Retrieved from http://hdl.handle.net/2292/3341

Chicago Manual of Style (16^th Edition):

Kowalczyk, Renata. “Synthesis of mannosylated peptides as components for synthetic vaccines.” 2008. Doctoral Dissertation, University of Auckland. Accessed January 18, 2021. http://hdl.handle.net/2292/3341.

MLA Handbook (7^th Edition):

Kowalczyk, Renata. “Synthesis of mannosylated peptides as components for synthetic vaccines.” 2008. Web. 18 Jan 2021.

Vancouver:

Kowalczyk R. Synthesis of mannosylated peptides as components for synthetic vaccines. [Internet] [Doctoral dissertation]. University of Auckland; 2008. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/2292/3341.

Council of Science Editors:

Kowalczyk R. Synthesis of mannosylated peptides as components for synthetic vaccines. [Doctoral Dissertation]. University of Auckland; 2008. Available from: http://hdl.handle.net/2292/3341

NSYSU

6. Chao, Wen-yu. Synthesis of Cyclic Peptide Inhibitors for Grb2-SH2 via Ring-Closing Metathesis in Water.

Degree: Master, Chemistry, 2017, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0721117-143402

► Due to the good affinity and selectivity of cyclic peptides towards their targets, these have been reported to be therapeutic agents that can be used… (more)

Subjects/Keywords: Grb2-SH2 inhibitors; cyclic peptide; Ras protein; solid-phase peptide synthesis; ring-closing metathesis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Chao, W. (2017). Synthesis of Cyclic Peptide Inhibitors for Grb2-SH2 via Ring-Closing Metathesis in Water. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0721117-143402

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Chao, Wen-yu. “Synthesis of Cyclic Peptide Inhibitors for Grb2-SH2 via Ring-Closing Metathesis in Water.” 2017. Thesis, NSYSU. Accessed January 18, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0721117-143402.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Chao, Wen-yu. “Synthesis of Cyclic Peptide Inhibitors for Grb2-SH2 via Ring-Closing Metathesis in Water.” 2017. Web. 18 Jan 2021.

Vancouver:

Chao W. Synthesis of Cyclic Peptide Inhibitors for Grb2-SH2 via Ring-Closing Metathesis in Water. [Internet] [Thesis]. NSYSU; 2017. [cited 2021 Jan 18]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0721117-143402.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Chao W. Synthesis of Cyclic Peptide Inhibitors for Grb2-SH2 via Ring-Closing Metathesis in Water. [Thesis]. NSYSU; 2017. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0721117-143402

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Mahatma Gandhi University

7. Koruthu, Desy P. Structure reactmty studies in crosslinked functionalised polymer supports and their applications in peptiide synthesis.

Degree: 1997, Mahatma Gandhi University

URL: http://shodhganga.inflibnet.ac.in/handle/10603/622

Bibliography and abbreviations are included Advisors/Committee Members: Pillai, V N Rajasekharan.

Subjects/Keywords: Solid Phase Peptide Synthesis; Polymer Synthesis

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APA (6^th Edition):

Koruthu, D. P. (1997). Structure reactmty studies in crosslinked functionalised polymer supports and their applications in peptiide synthesis. (Thesis). Mahatma Gandhi University. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/622

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Koruthu, Desy P. “Structure reactmty studies in crosslinked functionalised polymer supports and their applications in peptiide synthesis.” 1997. Thesis, Mahatma Gandhi University. Accessed January 18, 2021. http://shodhganga.inflibnet.ac.in/handle/10603/622.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Koruthu, Desy P. “Structure reactmty studies in crosslinked functionalised polymer supports and their applications in peptiide synthesis.” 1997. Web. 18 Jan 2021.

Vancouver:

Koruthu DP. Structure reactmty studies in crosslinked functionalised polymer supports and their applications in peptiide synthesis. [Internet] [Thesis]. Mahatma Gandhi University; 1997. [cited 2021 Jan 18]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/622.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Koruthu DP. Structure reactmty studies in crosslinked functionalised polymer supports and their applications in peptiide synthesis. [Thesis]. Mahatma Gandhi University; 1997. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/622

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

The Ohio State University

8. Gupta, Chitrak. Peptide Bond Geometry Studied by Solid-State NMR Spectroscopy.

Degree: MS, Chemistry, 2013, The Ohio State University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=osu1385331897

► Three-dimensional structure of proteins is an intrinsic property of every protein, and is directly related to its biological function. Studying protein structure is thus of… (more)

▼ Three-dimensional structure of proteins is an intrinsic property of every protein, and is directly related to its biological function. Studying protein structure is thus of immense importance to understand the mechanism by which the proteins perform such functions. In principle, the backbone structure of a protein can be completely described by a set of torsional angles. Indeed, a significant amount of structural studies of proteins have involved measuring the torsional angles defined by C'-N-Cα-C' atoms (denoted φ) and N-Cα-C'-N atoms (denoted ψ). Little has been done, however, to study the third of the triplet, the ω-torsional angle, defined by the Cα-C'-N-Cα atoms, which is usually assumed to be planar, with the atoms arranged in a trans-like conformation. However, cis-like peptide bonds are known to exist, often at or near active sites, which make them biologically significant, although there are no reliable experimental methods apart from crystallographic studies to differentiate cis-peptide bonds form their trans- counterparts.This thesis is aimed at developing a new solid-state NMR experiment to study the peptide bond geometry. The primary objective is to differentiate cis- and trans-peptide bonds in polypeptides and proteins. Chapter 2 describes the synthesis of isotopically labeled model compounds with trans- and cis-peptide bond. The former, glycylglycine, was synthesized by solid phase peptide synthesis following standard protocols, with minor modifications to increase the yield. The latter, 2,5-diketopiperazine, was synthesized using microwave-assisted synthesis, a protocol recently published. Both the compounds could be obtained in high purity and integrity, as shown by their solution 1H and 13C NMR spectra.Chapter 3 describes the design of the NMR pulse sequence which can achieve this goal. The fundamental idea is to allow correlated evolution of two different anisotropic interactions under magic-angle spinning. Such evolutions are sensitive to the relative orientation of the two interactions. In this case, 13Cα-15N dipolar coupling and 13C'-13Cα dipolar coupling have been correlated, to distinguish between the two geometries about the N-C' bond. The possibility of using 13C' chemical shift anisotropy instead of 13C'-13Cα dipolar coupling has been discussed. Preliminary simulations have shown the detection of a cis-peptide bond to be possible, although, thus far, the experiment is not sensitive enough to measure deviation from planarity of a peptide bond.Chapter 4 shows the solid-state NMR spectra obtained from these model compounds. The 1D 13C and 15N CP-MAS spectra from both the compounds confirm their purity and integrity, and provide sufficient signal-to-noise to proceed with the proposed experiment. REDOR dephasing recorded on both the compounds are in good agreement with simulation. Chapter 5 discusses the future perspectives of this work, including extending it to bigger systems, and to use 15N-1H dipolar coupling instead of 13C'-13Cα dipolar coupling to increase sensitivity and enable… Advisors/Committee Members: Jaroniec, Christopher (Advisor).

Subjects/Keywords: Chemistry; peptide bond geometry; cis-peptide bond; omega torsional angle; solid-state NMR spectroscopy; dipolar coupling; microwave-assisted synthesis; solid phase peptide synthesis

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APA (6^th Edition):

Gupta, C. (2013). Peptide Bond Geometry Studied by Solid-State NMR Spectroscopy. (Masters Thesis). The Ohio State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=osu1385331897

Chicago Manual of Style (16^th Edition):

Gupta, Chitrak. “Peptide Bond Geometry Studied by Solid-State NMR Spectroscopy.” 2013. Masters Thesis, The Ohio State University. Accessed January 18, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=osu1385331897.

MLA Handbook (7^th Edition):

Gupta, Chitrak. “Peptide Bond Geometry Studied by Solid-State NMR Spectroscopy.” 2013. Web. 18 Jan 2021.

Vancouver:

Gupta C. Peptide Bond Geometry Studied by Solid-State NMR Spectroscopy. [Internet] [Masters thesis]. The Ohio State University; 2013. [cited 2021 Jan 18]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1385331897.

Council of Science Editors:

Gupta C. Peptide Bond Geometry Studied by Solid-State NMR Spectroscopy. [Masters Thesis]. The Ohio State University; 2013. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1385331897

9. Ludolph, Björn. Festphasensynthese lipidierter Peptide und Benzodiazepindione.

Degree: 2004, Universität Dortmund

URL: http://hdl.handle.net/2003/5568

► Lipopeptide sind wertvolle Werkzeuge zur Aufklärung biologischer Fragestellungen im molekularen Detail. In dieser Arbeit wurden effiziente Festphasensynthese-Methoden zur Darstellung verschiedener Lipopeptide entwickelt. Schlüsselelement in der… (more)

Subjects/Keywords: Benzodiazepindione; benzodiazepinedions; Festphasensynthese; Lipopeptide; lipopeptids; Ras-Peptide; Ras-peptids; solid phase synthesis; 540

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APA (6^th Edition):

Ludolph, B. (2004). Festphasensynthese lipidierter Peptide und Benzodiazepindione. (Thesis). Universität Dortmund. Retrieved from http://hdl.handle.net/2003/5568

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ludolph, Björn. “Festphasensynthese lipidierter Peptide und Benzodiazepindione.” 2004. Thesis, Universität Dortmund. Accessed January 18, 2021. http://hdl.handle.net/2003/5568.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ludolph, Björn. “Festphasensynthese lipidierter Peptide und Benzodiazepindione.” 2004. Web. 18 Jan 2021.

Vancouver:

Ludolph B. Festphasensynthese lipidierter Peptide und Benzodiazepindione. [Internet] [Thesis]. Universität Dortmund; 2004. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/2003/5568.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ludolph B. Festphasensynthese lipidierter Peptide und Benzodiazepindione. [Thesis]. Universität Dortmund; 2004. Available from: http://hdl.handle.net/2003/5568

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Georgia

10. Chinoy, Zoeisha S. The chemoenzymatic synthesis of oligosaccharides and glycopeptides for functional glycomics.

Degree: 2014, University of Georgia

URL: http://hdl.handle.net/10724/30352

► Herein we describe a novel methodology for the synthesis of tri-antennary complex N-glycans. This methodology entails the use of a chemoenzymatic approach whereby a core… (more)

▼ Herein we describe a novel methodology for the synthesis of tri-antennary complex N-glycans. This methodology entails the use of a chemoenzymatic approach whereby a core pentasaccharide functionalized with the four orthogonal protecting groups - levulinoyl (Lev), fluorenylmethyloxycarbonate (Fmoc), allyloxycarbonate (Alloc), and 2-naphthylmethyl (Nap), at key branching positions, was synthesized chemically. Upon selective deprotection of those orthogonal protecting groups, three unique saccharide structures were attached by chemical glycosylations to form two decasaccharides with different branching patterns. The decasaccharides were used as precursors for enzymatic extension using glycosyltransferases, which yielded a library of various symmetrical and asymmetrical complex N-glycans. The novelty of this approach enatils the judicious manipulation of the substrate specificities of various glycosyltransferases, which donot recognize N-acetyllactosmine masked with acetyl protecting groups. Furthermore, certain glycosyltransferases do not recognize terminal N-acetylglucosamine residues as a substrate and hence renders it inactive towards glycosylation. However upon removal of the acetyl esters, and conversion of the N- acetylglucosamine residue to an N-acetyllactosmine, it would serve as a substrate for enzymatic modification for further elaboration of the glycan. Using this novel chemoenzymatic approach, a library of glycans were synthesized, which were printed as microarrays and screened for binding to lectins and influenza-virus hemagglutinins, which showed that recognition is modulated by presentation of minimal epitopes in the context of complex N-glycans. We employed the use of our novel chemoenzymatic methodology for the synthesis of triantennary glycans found on human egg cell zona pellucidas (ZPs), to study the binding affinities of the ZP glycans to the sperm. The preliminary results indicated that the presence of Sialyl Lewisx (SLex) on an extended branch as a SLexLex moiety enhanced the inhibition. We also, report the chemical synthesis of the core trisaccharide-glycopeptide found as a posttranslational modification on a specific hydroxyproline (HyPro) residue of the protein Skp1, in an organism called Dictyostelium. The trisaccharide-HyPro and trisaccharide- glycopeptide were used to study the substrate specificity for the enzyme AgtA in order to ascertain its substrate specificity. The synthetic trisaccharide-glycopeptide was conjugated to a carrier protein (KLH), which will be used for generating monoclonal antibodies to study glycosylation changes during development in Dictyostelium.

Subjects/Keywords: Oligosaccharides; Glycans; Glycosylation; Glycosyltransferases; Glycopeptides; Solid Phase Peptide Synthesis; Microarray; Influenza; Fertilization; Skp1.

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APA (6^th Edition):

Chinoy, Z. S. (2014). The chemoenzymatic synthesis of oligosaccharides and glycopeptides for functional glycomics. (Thesis). University of Georgia. Retrieved from http://hdl.handle.net/10724/30352

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Chinoy, Zoeisha S. “The chemoenzymatic synthesis of oligosaccharides and glycopeptides for functional glycomics.” 2014. Thesis, University of Georgia. Accessed January 18, 2021. http://hdl.handle.net/10724/30352.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Chinoy, Zoeisha S. “The chemoenzymatic synthesis of oligosaccharides and glycopeptides for functional glycomics.” 2014. Web. 18 Jan 2021.

Vancouver:

Chinoy ZS. The chemoenzymatic synthesis of oligosaccharides and glycopeptides for functional glycomics. [Internet] [Thesis]. University of Georgia; 2014. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/10724/30352.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Chinoy ZS. The chemoenzymatic synthesis of oligosaccharides and glycopeptides for functional glycomics. [Thesis]. University of Georgia; 2014. Available from: http://hdl.handle.net/10724/30352

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of KwaZulu-Natal

11. Ramkisson, Shaveer. Solid phase peptide synthesis: new resin and new protecting group.

Degree: 2018, University of KwaZulu-Natal

URL: https://researchspace.ukzn.ac.za/handle/10413/18079

► Solid phase peptide synthesis is the common approach used today in synthesizing peptides in a research scale and production. Success in this approach are governed… (more)

▼ Solid phase peptide synthesis is the common approach used today in synthesizing peptides in a research scale and production. Success in this approach are governed by several factors. These are; (1) the solid support on which synthesis is to be carried out, (2) linker/spacer on which the first amino acid is linked to the support to allow stepwise growth of the peptide chain, (3) protecting groups of amino acids to allow a clean synthesis without disruption of the growing peptide chain and (4) coupling reagents for improved amide bond formation yielding peptides in the shortest amount of time and with the highest quality. The following thesis shows work conducted on some of these aspects. Chapter two is based on the application of a novel resin Fmoc-Rink-Amide PEG Octagel surface resin. Chapter three describes the development of a novel protecting group for histidine amino acid. A new PEG-PS based resin called Octagel has been developed by Aapptec. Fmoc-Rink-Amide Octagel PEG surface resin is a unique highly uniform surface-active resin. To study the resin’s performance, two peptides Aib-enkephalin pentapeptide and Aib-ACP decapeptide were synthesized and results were compared to Polystyrene and ChemMatrix resins. Swelling and microscope imaging studies were also conducted on each of the resins to highlight their performance associated with Solid Phase Peptide Synthesis. Results have demonstrated that Octagel resin has the potential to synthesize peptide sequences with high purity and therefore to be a good alternative to those currently in the market. Histidine is an important amino acid used in SPPS. It contains a reactive imidazole side group that can cause side reactions in SPPS if left unprotected. Fmoc-based SPPS is the most commonly used strategy in synthesizing peptides today. A protecting group was created for Fmoc-Histidine. SPPS was carried out on the protected histidine and results show that the group is stable in acidic conditions. Advisors/Committee Members: Albericio, Fernando. (advisor), Garcia-de-la-Torre, Beatriz. (advisor).

Subjects/Keywords: Solid phase.; Peptide synthesis.; New resin group.; New protecting group.; Amino acids.

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APA (6^th Edition):

Ramkisson, S. (2018). Solid phase peptide synthesis: new resin and new protecting group. (Thesis). University of KwaZulu-Natal. Retrieved from https://researchspace.ukzn.ac.za/handle/10413/18079

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ramkisson, Shaveer. “Solid phase peptide synthesis: new resin and new protecting group.” 2018. Thesis, University of KwaZulu-Natal. Accessed January 18, 2021. https://researchspace.ukzn.ac.za/handle/10413/18079.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ramkisson, Shaveer. “Solid phase peptide synthesis: new resin and new protecting group.” 2018. Web. 18 Jan 2021.

Vancouver:

Ramkisson S. Solid phase peptide synthesis: new resin and new protecting group. [Internet] [Thesis]. University of KwaZulu-Natal; 2018. [cited 2021 Jan 18]. Available from: https://researchspace.ukzn.ac.za/handle/10413/18079.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ramkisson S. Solid phase peptide synthesis: new resin and new protecting group. [Thesis]. University of KwaZulu-Natal; 2018. Available from: https://researchspace.ukzn.ac.za/handle/10413/18079

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Toronto

12. Weissman, Max Ryan. Synthetic Strategies in Solid-Phase Peptide Synthesis: N-Terminus Modification.

Degree: 2018, University of Toronto

URL: http://hdl.handle.net/1807/99954

►

Solid-phase peptide synthesis (SPPS) is a technique used in the synthesis of peptides. SPPS is mainly used to synthesize linear peptides using natural Îą-amino acid… (more)

Subjects/Keywords: Carbodiimide; Coupling conditions; Isocyanate; N-terminus modification; Solid-phase peptide synthesis; 0490

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Weissman, M. R. (2018). Synthetic Strategies in Solid-Phase Peptide Synthesis: N-Terminus Modification. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/99954

Chicago Manual of Style (16^th Edition):

Weissman, Max Ryan. “Synthetic Strategies in Solid-Phase Peptide Synthesis: N-Terminus Modification.” 2018. Masters Thesis, University of Toronto. Accessed January 18, 2021. http://hdl.handle.net/1807/99954.

MLA Handbook (7^th Edition):

Weissman, Max Ryan. “Synthetic Strategies in Solid-Phase Peptide Synthesis: N-Terminus Modification.” 2018. Web. 18 Jan 2021.

Vancouver:

Weissman MR. Synthetic Strategies in Solid-Phase Peptide Synthesis: N-Terminus Modification. [Internet] [Masters thesis]. University of Toronto; 2018. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/1807/99954.

Council of Science Editors:

Weissman MR. Synthetic Strategies in Solid-Phase Peptide Synthesis: N-Terminus Modification. [Masters Thesis]. University of Toronto; 2018. Available from: http://hdl.handle.net/1807/99954

University of Georgia

13. Wu, Dong. Chemotactic peptides encoded by hiv-1 nef gene.

Degree: 2014, University of Georgia

URL: http://hdl.handle.net/10724/21726

► Preliminary experimental evidence showed the potential for formation of a new isoform of the nef gene in HIV-1 by –1 ribosomal frameshifting. We have named… (more)

Subjects/Keywords: Frameshift; HIV-1; nef; nef-fs; chemokine; solid phase synthesis; chemotaxis; calcium mobilizaion; synthesize; peptide

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wu, D. (2014). Chemotactic peptides encoded by hiv-1 nef gene. (Thesis). University of Georgia. Retrieved from http://hdl.handle.net/10724/21726

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Wu, Dong. “Chemotactic peptides encoded by hiv-1 nef gene.” 2014. Thesis, University of Georgia. Accessed January 18, 2021. http://hdl.handle.net/10724/21726.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Wu, Dong. “Chemotactic peptides encoded by hiv-1 nef gene.” 2014. Web. 18 Jan 2021.

Vancouver:

Wu D. Chemotactic peptides encoded by hiv-1 nef gene. [Internet] [Thesis]. University of Georgia; 2014. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/10724/21726.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Wu D. Chemotactic peptides encoded by hiv-1 nef gene. [Thesis]. University of Georgia; 2014. Available from: http://hdl.handle.net/10724/21726

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Western Ontario

14. Charlton, Carlie L. The Design and Synthesis of Ghrelin Analogues as Non‐Invasive GHS‐R1a Imaging Probes.

Degree: 2016, University of Western Ontario

URL: https://ir.lib.uwo.ca/etd/3732

► The field of molecular imaging is constantly growing and evolving in order to provide the best possible healthcare for patients in various stages of disease… (more)

▼ The field of molecular imaging is constantly growing and evolving in order to provide the best possible healthcare for patients in various stages of disease and therapy. Molecular imaging aims to locate specific markers of disease by selectively targeting the markers of interest with high selectivity and visualizing the accumulation using external detection. The growth hormone secretagogue receptor-1a (GHS-R1a) has been shown to be involved in various important biological functions such as energy homeostasis and cardiac contractility. GHS-R1a has shown involvement in proliferation, migration and cell invasion of specific cancer subtypes. Therefore, targeting GHS-R1a is an important marker of different disease states and would be advantageous to selectively target for diagnostic and therapeutic purposes. This thesis will document the development of peptide-based molecular imaging agents capable of targeting GHS-R1a with high affinity designed off the structure of ghrelin, the endogenous ligand for GHS-R1a. Chapter 2 discusses the synthesis and evaluation of gallium-69/71 and gallium-68 labelled ghrelin(1-19) analogues. The first generation of ghrelin analogues was designed to detect GHS-R1a by positron emission tomography (PET). Chelation of gallium had a positive effect on binding affinity to GHS-R1a resulting in an IC50 comparable to natural ghrelin(1-28). Preclinical evaluation of HT1080/GHSR-1a xenografts showed higher SUVR values than the HT1080 xenograft with no GHS-R1a. Chapter 3 discusses the second generation of ghrelin analogues that were further truncated to eight amino acids. A structural activity study investigated residues 1, 3, 4, and 8 to determine whether amino acid substitutions produce the best binding affinity GHS-R1a. The optimized ghrelin analogue has 12-fold higher binding affinity to GHS-R1a than natural ghrelin. New radiochemical syntheses were reported for a 6-[ 18F]-fluoro-2-pentafluorophenylnaphthoate prosthetic group. The lead peptide analogue was radiolabelled in a 3% radiochemical yield and resulted in the first fluorine-18 labelled ghrelin(1-8) analogue with greater affinity to GHS-R1a. ii The final chapter describes the effects of targeting GHS-R1a with a dimerized ghrelin(1-8) peptide. Dimerizing other peptide targeting entities has increased binding affinity to the target however, this is not the case found with ghrelin. The in vitro kinetics were evaluated using fluorescence microscopy in GHS-R1a expressing cells. All three chapters discuss the systematic modification of an endogenous peptide ligand into a high affinity, PET imaging agent through classical methods of peptide modification and radiochemistry.

Subjects/Keywords: Ghrelin; GHS‐R1a; Peptide; Molecular Imaging; PET; Optical Imaging; Solid‐Phase Peptide Synthesis; Medicinal-Pharmaceutical Chemistry; Organic Chemistry; Radiochemistry

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Charlton, C. L. (2016). The Design and Synthesis of Ghrelin Analogues as Non‐Invasive GHS‐R1a Imaging Probes. (Thesis). University of Western Ontario. Retrieved from https://ir.lib.uwo.ca/etd/3732

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Charlton, Carlie L. “The Design and Synthesis of Ghrelin Analogues as Non‐Invasive GHS‐R1a Imaging Probes.” 2016. Thesis, University of Western Ontario. Accessed January 18, 2021. https://ir.lib.uwo.ca/etd/3732.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Charlton, Carlie L. “The Design and Synthesis of Ghrelin Analogues as Non‐Invasive GHS‐R1a Imaging Probes.” 2016. Web. 18 Jan 2021.

Vancouver:

Charlton CL. The Design and Synthesis of Ghrelin Analogues as Non‐Invasive GHS‐R1a Imaging Probes. [Internet] [Thesis]. University of Western Ontario; 2016. [cited 2021 Jan 18]. Available from: https://ir.lib.uwo.ca/etd/3732.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Charlton CL. The Design and Synthesis of Ghrelin Analogues as Non‐Invasive GHS‐R1a Imaging Probes. [Thesis]. University of Western Ontario; 2016. Available from: https://ir.lib.uwo.ca/etd/3732

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Melbourne

15. BELGI, ALESSIA. Chemical synthesis and structure-function relationship study of insulin-like peptide 5 (INSL5).

Degree: 2012, University of Melbourne

URL: http://hdl.handle.net/11343/37299

► Insulin-like peptide 5 (INSL5) is a member of the insulin/relaxin superfamily of peptides. It is composed of two chains, A and B, linked together by… (more)

▼ Insulin-like peptide 5 (INSL5) is a member of the insulin/relaxin superfamily of peptides. It is composed of two chains, A and B, linked together by two interchain disulfide bonds. A third intrachain disulfide bond exists within the A-chain. It has been identified as the cognate ligand for the G-protein-coupled receptor RXFP4. Although the complete physiological role of this naturally occurring peptide is still under investigation, there is evidence that it acts to both stimulate appetite and activate colon motility. Therefore both peptide agonists and antagonists may have potential important therapeutic applications. To further investigate the biochemical role of this peptide and because of the ready availability of the mouse as an experimental animal, the chemical synthesis of mouse INSL5 was undertaken. Due to its complex structure and the intractable nature of the two constituent chains, different solid phase synthesis strategies were investigated including the use of a temporary B-chain solubilising tag. Unfortunately, none provided significantly improved yield of purified mouse INSL5 which reflects the complexity of this peptide. A mouse INSL5 analogue with its two chains as C-terminal amides was also prepared. It was found that mouse INSL5 amide was substantially less potent than the native acid form highlighting the necessity for free C-terminal carboxylates for function. The structure-function relationship study continued with the design of two additional mouse INSL5 analogues. One had mutations targeting residues potentially involved in receptor activation (mouse INSL5 A-B(R24A, W25A)) and the other with the substitution of the residues potentially involved in the binding (mouse INSL5 A-B(K6A, R14A, Y18A)). The bioassays results showed that the Arg-Trp pair at the C-terminus of the B-chain is indeed important for activation and that LysB6, ArgB14 and TyrB18 are some of the key residues for receptor binding. The synthesis of the point-mutated analogues was again very challenging despite the application of various strategies. To potentially bypass this ongoing difficulty, biologically active single chain analogues were sought. A series of single B- and A-chain analogues were synthesized but none showed biological activity within the concentration range tested suggesting that INSL5 likely requires two chains for bioactivity. Next, minimization of native two-chain mouse INSL5 was examined. Mouse INSL5 A(C7A, C12A)-B and mouse INSL5 A(8-21, C12A)-B were prepared. The intramolecular disulfide bond was removed from both analogues and one had an additional N-terminus truncation on the A-chain. The synthesis of both analogues was simpler compared to other INSL5 peptides and they were also able to rescue both affinity and activity compared to the single chain analogues. They were also active in vivo, representing attractive templates for further modification. …

Subjects/Keywords: insulin-like peptide 5; solid phase peptide synthesis; regioselective disulfide bond formation; in vitro characterization; minimized analogues design

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

BELGI, A. (2012). Chemical synthesis and structure-function relationship study of insulin-like peptide 5 (INSL5). (Doctoral Dissertation). University of Melbourne. Retrieved from http://hdl.handle.net/11343/37299

Chicago Manual of Style (16^th Edition):

BELGI, ALESSIA. “Chemical synthesis and structure-function relationship study of insulin-like peptide 5 (INSL5).” 2012. Doctoral Dissertation, University of Melbourne. Accessed January 18, 2021. http://hdl.handle.net/11343/37299.

MLA Handbook (7^th Edition):

BELGI, ALESSIA. “Chemical synthesis and structure-function relationship study of insulin-like peptide 5 (INSL5).” 2012. Web. 18 Jan 2021.

Vancouver:

BELGI A. Chemical synthesis and structure-function relationship study of insulin-like peptide 5 (INSL5). [Internet] [Doctoral dissertation]. University of Melbourne; 2012. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/11343/37299.

Council of Science Editors:

BELGI A. Chemical synthesis and structure-function relationship study of insulin-like peptide 5 (INSL5). [Doctoral Dissertation]. University of Melbourne; 2012. Available from: http://hdl.handle.net/11343/37299

University of Illinois – Chicago

16. Wang, Songlin. Solid-State Nuclear Magnetic Resonance of Protein Characterization at Fast Magic Angle Spinning.

Degree: 2016, University of Illinois – Chicago

URL: http://hdl.handle.net/10027/20151

► In the thesis studies, three related projects using SSNMR were carried out on proteins and peptides in solids. In the first project, we implemented high-resolution… (more)

Subjects/Keywords: Solid-state NMR; Protein characterization; Proton detection; HIGHLIGHT REDOR; Ultra-fast magic angle spinning; solid phase peptide synthesis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wang, S. (2016). Solid-State Nuclear Magnetic Resonance of Protein Characterization at Fast Magic Angle Spinning. (Thesis). University of Illinois – Chicago. Retrieved from http://hdl.handle.net/10027/20151

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Wang, Songlin. “Solid-State Nuclear Magnetic Resonance of Protein Characterization at Fast Magic Angle Spinning.” 2016. Thesis, University of Illinois – Chicago. Accessed January 18, 2021. http://hdl.handle.net/10027/20151.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Wang, Songlin. “Solid-State Nuclear Magnetic Resonance of Protein Characterization at Fast Magic Angle Spinning.” 2016. Web. 18 Jan 2021.

Vancouver:

Wang S. Solid-State Nuclear Magnetic Resonance of Protein Characterization at Fast Magic Angle Spinning. [Internet] [Thesis]. University of Illinois – Chicago; 2016. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/10027/20151.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Wang S. Solid-State Nuclear Magnetic Resonance of Protein Characterization at Fast Magic Angle Spinning. [Thesis]. University of Illinois – Chicago; 2016. Available from: http://hdl.handle.net/10027/20151

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Université de Bordeaux I

17. Sani, Marc Antoine. Apoptosis regulation via the mitochondrial pathway : membrane response upon apoptotic stimuli : Régulation de l'apoptose au niveau mitochondrial : réponse membranaire à des stimuli apoptotiques.

Degree: Docteur es, Chimie-physique, 2008, Université de Bordeaux I

URL: http://www.theses.fr/2008BOR13651

►

Le but de cette thèse est de montrer la réponse de la membrane mitochondriale au cours la régulation de l’apoptose en étudiant l’effet de domaines… (more)

▼

Le but de cette thèse est de montrer la réponse de la membrane mitochondriale au cours la régulation de l’apoptose en étudiant l’effet de domaines clés sur la dynamique membranaire et l’importance de la composition phospholipidiques des modèles utilisés. Le domaine BH4 est la partie spécifique anti-apoptotique de la famille Bcl-2. La première étape a été de synthétiser le peptide par voie chimique en utilisant la synthèse peptidique en phase solide. Un protocole décrivant les étapes de purification par chromatographie liquide et de caractérisation par spectroscopie de masse, garantissant une pureté indispensable pour des études biophysiques, a été établi. La modification de la structure secondaire du peptide interagissant avec des vésicules a été étudiée par spectroscopie infrarouge ainsi que par dichroïsme circulaire. Le peptide s’agrège à la surface et s’insère peu profondément dans la partie hydrophobe de la membrane. En utilisant la résonance magnétique nucléaire (RMN) et la calorimétrie, il a été montré que le peptide BH4 modifie l’organisation et la dynamique des liposomes mimant la surface mitochondriale. La deuxième étude a porté sur la première hélice de la protéine pro-apoptotique Bax (Bax-a1) qui a la propriété de diriger la protéine cytosolique vers la mitochondrie. Un protocole de synthèse et purification a été à nouveau établi. Le but de cette étude est de démontrer le rôle de l’interaction spécifique entre la cardiolipine, un phospholipide uniquement présent dans la mitochondrie et le peptide Bax-a1. Les études RMN ont montré que Bax-a1 n’interagissait uniquement que si la cardiolipine était présente, produisant un fort effet électrostatique piégeant le peptide à la surface de la membrane. Enfin, un nouveau protocole permettant d’étudier la réponse des lipides de mitochondries isolées toujours actives par RMN est présenté. Le but est de pouvoir directement observer les modifications subies par chaque phospholipide de la mitochondrie. .

The aim of this thesis was the investigation of the mitochondrial response mechanisms upon apoptotic stimuli. The specific objectives were the biophysical characterization of membrane dynamics and the specific roles of lipids in the context of apoptotic regulation occurring at the mitochondrion and its complex membrane systems. The BH4 domain is an anti-apoptotic specific domain of the Bcl-2 protein. Solid phase peptide synthesis was used to produce large amount of the peptide for biophysical studies. A protocol has been established and optimized, guarantying the required purity for biophysical studies. In detail the purification by high performance liquid chromatography and the characterisation via mass spectroscopy are described. The secondary structure of BH4 changes significantly in the presence of lipid vesicles as observed by infrared spectroscopy and circular dichroism. The BH4 peptide aggregates at the membrane surface and inserts slightly into the hydrophobic part of the membrane. Using nuclear magnetic resonance (NMR) and calorimetry techniques, it could…

Advisors/Committee Members: Dufourc, Erick Joël (thesis director), Gröbner, Gerhard (thesis director).

Subjects/Keywords: Apoptose; Cardiolipine; Peptide BH4; Synthèse peptidique en phase solide; Peptide Bax-a1; Dichroïsme circulaire; Modèle de membrane; RMN des solides; Apoptosis; BH4 peptide; Bax-a1 peptide; Model membrane; Cardiolipin; Solid phase peptide synthesis; Circular dichroism; Solid-state nuclear magnetic; Resonance spectroscopy

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sani, M. A. (2008). Apoptosis regulation via the mitochondrial pathway : membrane response upon apoptotic stimuli : Régulation de l'apoptose au niveau mitochondrial : réponse membranaire à des stimuli apoptotiques. (Doctoral Dissertation). Université de Bordeaux I. Retrieved from http://www.theses.fr/2008BOR13651

Chicago Manual of Style (16^th Edition):

Sani, Marc Antoine. “Apoptosis regulation via the mitochondrial pathway : membrane response upon apoptotic stimuli : Régulation de l'apoptose au niveau mitochondrial : réponse membranaire à des stimuli apoptotiques.” 2008. Doctoral Dissertation, Université de Bordeaux I. Accessed January 18, 2021. http://www.theses.fr/2008BOR13651.

MLA Handbook (7^th Edition):

Sani, Marc Antoine. “Apoptosis regulation via the mitochondrial pathway : membrane response upon apoptotic stimuli : Régulation de l'apoptose au niveau mitochondrial : réponse membranaire à des stimuli apoptotiques.” 2008. Web. 18 Jan 2021.

Vancouver:

Sani MA. Apoptosis regulation via the mitochondrial pathway : membrane response upon apoptotic stimuli : Régulation de l'apoptose au niveau mitochondrial : réponse membranaire à des stimuli apoptotiques. [Internet] [Doctoral dissertation]. Université de Bordeaux I; 2008. [cited 2021 Jan 18]. Available from: http://www.theses.fr/2008BOR13651.

Council of Science Editors:

Sani MA. Apoptosis regulation via the mitochondrial pathway : membrane response upon apoptotic stimuli : Régulation de l'apoptose au niveau mitochondrial : réponse membranaire à des stimuli apoptotiques. [Doctoral Dissertation]. Université de Bordeaux I; 2008. Available from: http://www.theses.fr/2008BOR13651

Temple University

18. Kheirabadi, Mahboubeh. Inside-out design and synthesis of spiroligomers for transesterification reactions.

Degree: PhD, 2014, Temple University

URL: http://digital.library.temple.edu/u?/p245801coll10,304046

►

Chemistry

This work describes the application of spiroligomers as serine hydrolases mimetics. Through collaboration with Kendall Houk's group, for the first time in the Schafmeister… (more)

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Chemistry

This work describes the application of spiroligomers as serine hydrolases mimetics. Through collaboration with Kendall Houk's group, for the first time in the Schafmeister lab, we demonstrate that "theozymes" can be successfully used as models to design highly functionalized spiroligomer constructs for organocatalysis. We demonstrate a structure-function relationship between the structure of a series of bi-functional and tri-functional spiroligomer based transesterification catalysts and their catalytic activity. First, we designed and synthesized a series of stereochemically and regiochemically diverse bi-functional spiroligozymes to identify the best arrangement of a pyridine as a general base catalyst and an alcohol nucleophile to accelerate attack on vinyl trifluoroacetate as an electrophile. The best bifunctional spiroligozyme reacts with vinyl trifluoroacetate to form an acyl-spiroligozyme conjugate 2.7x103-fold faster than the background reaction with benzyl alcohol. We then incorporated an additional urea functional group to activate the acyl-spiroligozyme intermediate through hydrogen bonds and enable acyl transfer to methanol. The best trifunctional spiroligozyme carries out multiple turnovers and acts as a transesterification catalyst with k1/kuncat of 2.2x103 and k2/kuncat of 1.3x102. Quantum mechanical calculations identified four transition states in the catalytic cycle and provided a detailed view of every stage of the transesterification reaction. With the aim of accelerating the k2, we sought to design better oxyanion holes that hold multiple hydrogen bonding groups in close proximity of the catalytic groups. A macrocyclic motif would be a good candidate to force the oxyanion hole arm to arrange hydrogen-bonding groups in a precise three-dimensional constellation for transition state stabilization. In Chapter 4, we introduce an in silico designed macrocyclic spiroligomer, which overlays well with catalytic active site of an inhibitor bound-esterase. Finally, we detail our effort to develop new methodologies for rapidly synthesizing spiroligomers on solid-support. This would allow us to efficiently permute their structures for diverse applications such as organocatalysts, host molecules, and biologically related applications such as inhibiting protein-protein interactions.

Temple University – Theses

Advisors/Committee Members: Schafmeister, Christian., Andrade, Rodrigo B.;, Andrade, Rodrigo B., Wuest, William M., Schafmeister, Christian, Murray, James K.;.

Subjects/Keywords: Chemistry; Organic chemistry;

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APA (6^th Edition):

Kheirabadi, M. (2014). Inside-out design and synthesis of spiroligomers for transesterification reactions. (Doctoral Dissertation). Temple University. Retrieved from http://digital.library.temple.edu/u?/p245801coll10,304046

Chicago Manual of Style (16^th Edition):

Kheirabadi, Mahboubeh. “Inside-out design and synthesis of spiroligomers for transesterification reactions.” 2014. Doctoral Dissertation, Temple University. Accessed January 18, 2021. http://digital.library.temple.edu/u?/p245801coll10,304046.

MLA Handbook (7^th Edition):

Kheirabadi, Mahboubeh. “Inside-out design and synthesis of spiroligomers for transesterification reactions.” 2014. Web. 18 Jan 2021.

Vancouver:

Kheirabadi M. Inside-out design and synthesis of spiroligomers for transesterification reactions. [Internet] [Doctoral dissertation]. Temple University; 2014. [cited 2021 Jan 18]. Available from: http://digital.library.temple.edu/u?/p245801coll10,304046.

Council of Science Editors:

Kheirabadi M. Inside-out design and synthesis of spiroligomers for transesterification reactions. [Doctoral Dissertation]. Temple University; 2014. Available from: http://digital.library.temple.edu/u?/p245801coll10,304046

Mahatma Gandhi University

19. Varkey, Jaya T. Synthesis of thioredoxin partial sequences on a hydrophilic crosslinked polymeric support.

Degree: 2010, Mahatma Gandhi University

URL: http://shodhganga.inflibnet.ac.in/handle/10603/360

References p.130-140 Advisors/Committee Members: Pillai, V N Rajasekharan.

Subjects/Keywords: Peptide Chemisty; Synthetic Peptides; Solid Phase Synthesis

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APA (6^th Edition):

Varkey, J. T. (2010). Synthesis of thioredoxin partial sequences on a hydrophilic crosslinked polymeric support. (Thesis). Mahatma Gandhi University. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/360

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Varkey, Jaya T. “Synthesis of thioredoxin partial sequences on a hydrophilic crosslinked polymeric support.” 2010. Thesis, Mahatma Gandhi University. Accessed January 18, 2021. http://shodhganga.inflibnet.ac.in/handle/10603/360.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Varkey, Jaya T. “Synthesis of thioredoxin partial sequences on a hydrophilic crosslinked polymeric support.” 2010. Web. 18 Jan 2021.

Vancouver:

Varkey JT. Synthesis of thioredoxin partial sequences on a hydrophilic crosslinked polymeric support. [Internet] [Thesis]. Mahatma Gandhi University; 2010. [cited 2021 Jan 18]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/360.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Varkey JT. Synthesis of thioredoxin partial sequences on a hydrophilic crosslinked polymeric support. [Thesis]. Mahatma Gandhi University; 2010. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/360

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

20. Xu, Jingjing. Solid-phase synthesis of molecularly imprinted polymer nanoparticles for protein recognition : Synthèse en phase solide de nanoparticules de polymères à empreintes moléculaires pour la reconnaissance de protéines.

Degree: Docteur es, Biotechnology : Unité de recherche Génie Enzymatique et Cellulaire - GEC (FRE-3580), 2017, Compiègne

URL: http://www.theses.fr/2017COMP2349

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Cette thèse décrit la synthèse de nanoparticules de polymères à empreintes moléculaires (MIP, de l’anglais molecularly imprinted polymer) pour la reconnaissance de protéines, par une… (more)

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Cette thèse décrit la synthèse de nanoparticules de polymères à empreintes moléculaires (MIP, de l’anglais molecularly imprinted polymer) pour la reconnaissance de protéines, par une approche de synthèse en phase solide. Les polymères à empreintes moléculaires sont des récepteurs biomimétiques synthétisés sur mesure par un processus de nanomoulage du polymère autour de la molécule unique. Ils possèdent ainsi des cavités de reconnaissance spécifiques pour leur molécule cible. La technique de l'impression moléculaire pour les petites molécules cibles est bien établie, alors que l'impression de protéines reste encore un défi en raison de la flexibilité et complexité de leur structure native et de leurs nombreux sites fonctionnels, mais aussi en raison de leur faible stabilité dans des conditions inhabituelles. Par conséquent, une approche de synthèse en phase solide a été développée ici où la protéine est immobilisée sur un support avant la synthèse de nanoparticules hydrosolubles de MIP par polymérisation radicalaire. Les MIPs obtenus ont des affinités comparables à celles des anticorps, et des réactivités croisées faibles. Ils possèdent des avantages tels qu'une stabilité meilleure, un coût plus faible et peuvent potentiellement être régénérés et réutilisés, devenant ainsi des alternatives prometteuses aux anticorps naturels. Nous avons fabriqué des MIPs contre des protéases à sérine, telles la trypsine et la kallikréine, mais aussi contre un épitope peptidique de la protéine gp41 du VIH. Des nanogels de MIP thermosensibles ont été synthétisés dans un réacteur sous la forme d’une colonne thermostatée ou une boîte de Pétri, par polymérisation radicalaire initiée par voie thermique ou photochimique. Un simple changement de la température permet de libérer les MIPs de la protéine immobilisée. Ces MIPs sont hydrosolubles en fonction de la température et ont un diamètre inférieur à 100 nm. Leur affinité pour leur cible est élevée, avec un Kd du nano ou picomolaire. Ces 'anticorps synthétiques' ont été appliqués dans des tests d'adsorption sur microbalance à cristal de quartz, mais également comme 'chaperons synthétiques'. Des études préliminaires de la protection des protéines d'une dénaturation thermique ou par un pH défavorable ont été effectuées. L'utilisation d'un iniferter pour initier la photopolymérisation vivante du MIP a permis de synthétiser des nanogels de type core-shell. En introduisant des marqueurs fluorescents dans les MIPs, les tests d’immunoessai dans des fluides biologiques ont été démontrés, ce qui indique le grand potentiel de ces MIPs dans le diagnostic clinique. En conclusion, nous avons développé une nouvelle approche de synthèse de nanoparticules de MIP hydrosoluble ayant une haute affinité pour une protéine, utilisables à la place des anticorps dans des applications dans le monde réel tel que la détection de protéines biomarqueurs dans des échantillons complexes, et potentiellement comme principe actif in vivo.

This thesis describes the synthesis, by a solid-phase synthesis approach, of…

Advisors/Committee Members: Tse Sum Bui, Jeanne Bernadette (thesis director), Haupt, Karsten (thesis director).

Subjects/Keywords: Anticorps plastiques; Récepteurs biomimétiques; Épitope peptidique; Molecularly imprinted polymer (MIP); Solid-phase synthesis; Protein recognition; Protein protection; Immunoassay; Biosensing; Peptide epitope

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Xu, J. (2017). Solid-phase synthesis of molecularly imprinted polymer nanoparticles for protein recognition : Synthèse en phase solide de nanoparticules de polymères à empreintes moléculaires pour la reconnaissance de protéines. (Doctoral Dissertation). Compiègne. Retrieved from http://www.theses.fr/2017COMP2349

Chicago Manual of Style (16^th Edition):

Xu, Jingjing. “Solid-phase synthesis of molecularly imprinted polymer nanoparticles for protein recognition : Synthèse en phase solide de nanoparticules de polymères à empreintes moléculaires pour la reconnaissance de protéines.” 2017. Doctoral Dissertation, Compiègne. Accessed January 18, 2021. http://www.theses.fr/2017COMP2349.

MLA Handbook (7^th Edition):

Xu, Jingjing. “Solid-phase synthesis of molecularly imprinted polymer nanoparticles for protein recognition : Synthèse en phase solide de nanoparticules de polymères à empreintes moléculaires pour la reconnaissance de protéines.” 2017. Web. 18 Jan 2021.

Vancouver:

Xu J. Solid-phase synthesis of molecularly imprinted polymer nanoparticles for protein recognition : Synthèse en phase solide de nanoparticules de polymères à empreintes moléculaires pour la reconnaissance de protéines. [Internet] [Doctoral dissertation]. Compiègne; 2017. [cited 2021 Jan 18]. Available from: http://www.theses.fr/2017COMP2349.

Council of Science Editors:

Xu J. Solid-phase synthesis of molecularly imprinted polymer nanoparticles for protein recognition : Synthèse en phase solide de nanoparticules de polymères à empreintes moléculaires pour la reconnaissance de protéines. [Doctoral Dissertation]. Compiègne; 2017. Available from: http://www.theses.fr/2017COMP2349

Virginia Tech

21. Chen, Xingguo Ronald. Pin1 Catalytic and WW Domain Ligands.

Degree: PhD, Chemistry, 2011, Virginia Tech

URL: http://hdl.handle.net/10919/37824

► Pin1 is a peptidyl prolyl isomerase (PPIase) enzyme with two domains, the catalytic domain and the WW domain. Both domains specifically bind pSer/pThrâ Pro motifs.… (more)

▼ Pin1 is a peptidyl prolyl isomerase (PPIase) enzyme with two domains, the catalytic domain and the WW domain. Both domains specifically bind pSer/pThrâ Pro motifs. Pin1 plays an important role in regulating the cell cycle, and it is involved in many diseases, such as cancer, HIV-1, Alzheimerâ s disease, asthma, hepatitis B, and rheumatoid arthritis. Pin1 is a very promising target for new drug development. Three stereoisomers: (R,S)-, (S,R)- and (S,S)-Acâ pSerâ Î¨[(Z)CH=C]â Pipâ 2-(2-naphthyl)ethylamine were synthesized as inhibitors binding to the Pin1 catalytic domain. The (R,S)- and (S,R)-isomers were synthesized via a 13-step route, with overall yields of 2.0% and 1.4%, respectively. The newly formed stereogenic center in the piperidyl ring was introduced by a Luche reduction, followed by a stereoselective [2,3]-Still-Wittig rearrangement. The configuration of the stereocenter was determined by NOESY of a bicyclic derivative. The (Z)- to (E)-alkene ratio in the rearrangement was (5.5:1). The (S,S)-isomer was obtained as the epimerized by-product resulting from the (S,R)-isomer in the Na/NH3 deprotection step. The IC50 values for Pin1 inhibition were: 52, 85, and 141 Î¼M, respectively. We concluded that in this Z-alkene isostere, the R-configuration would be preferred at both stereogenic centers, as mimics of L-Ser and L-Pip, to improve the affinity. Combinatorial chemistry is a powerful method to discover biologically active compounds, and solid-phase synthesis is most commonly used to synthesize combinatorial libraries. To identify ligands for the Pin1 WW domain, a library, R1COâ pSerâ Proâ NHR2, was designed. A new solid-phase phosphorylating reagent (SPPR) containing a phosphoramidite function was synthesized in one step from commercially available Wang resin. The SPPR was applied in the preparation of a designed library through parallel synthesis. The library contained 357 members (17 Ã 21), and was screened by an enzyme-linked enzyme binding assay (ELEBA). The best hits were resynthesized, and the competitive dissociation constants, Kd-rel, were measured by ELEBA, with a Kd-rel value of 130 Î¼M for the best ligand. The absolute dissociation constants will be measured by our collaborator, Prof. Jefferey Peng, University of Notre Dame, using NMR methods. Besides the identification of the Pin1 WW domain ligands, I created a practical method for solid-phase synthesis of phosphopeptides. Advisors/Committee Members: Etzkorn, Felicia A. (committeechair), Yee, Gordon T. (committee member), Tanko, James M. (committee member), Carlier, Paul R. (committee member), Gandour, Richard D. (committee member).

Subjects/Keywords: Pin1; catalytic; WW domain; ligand; inhibition; mimic; conformation; phosphorylation; assay; ELEBA; combinatorial; library; solid-phase; synthesis; NMR; peptide

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APA (6^th Edition):

Chen, X. R. (2011). Pin1 Catalytic and WW Domain Ligands. (Doctoral Dissertation). Virginia Tech. Retrieved from http://hdl.handle.net/10919/37824

Chicago Manual of Style (16^th Edition):

Chen, Xingguo Ronald. “Pin1 Catalytic and WW Domain Ligands.” 2011. Doctoral Dissertation, Virginia Tech. Accessed January 18, 2021. http://hdl.handle.net/10919/37824.

MLA Handbook (7^th Edition):

Chen, Xingguo Ronald. “Pin1 Catalytic and WW Domain Ligands.” 2011. Web. 18 Jan 2021.

Vancouver:

Chen XR. Pin1 Catalytic and WW Domain Ligands. [Internet] [Doctoral dissertation]. Virginia Tech; 2011. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/10919/37824.

Council of Science Editors:

Chen XR. Pin1 Catalytic and WW Domain Ligands. [Doctoral Dissertation]. Virginia Tech; 2011. Available from: http://hdl.handle.net/10919/37824

22. 山口, 浩; ヤマグチ, ヒロシ. Studies on the structures and ion transport activities of transmembrane domains for a design of peptide channel : Studies on the structures and ion transport activities of transmembrane domains for a design of peptide channel; Studies on the structures and ion transport activities of transmembrane domains for a design of peptide channel.

Degree: Saga University / 佐賀大学

URL: http://portal.dl.saga-u.ac.jp/handle/123456789/8437

本研究は、イオンチャンネルペプチドに関するAib残基の役割を明らかにする事を目的とした。その結果、ペプチド中のAib残基はペプチドの会合体構造の安定化、リン脂質膜との親和性の増加に寄与していた。またAib残基の導入位置が抗菌作用、およびイオンチャンネル形成のための重要な因子である事を明らかにした。以上の結果はAibペプチドの構造機能に重要な示唆を与え、今後の非タンパク質性アミノ酸研究に寄与すると期待される。

Subjects/Keywords: Ion Channel; Transmembrane; Peptide; 2-aminoisobutyric Acid; Solid-Phase Peptide Synthesis

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APA (6^th Edition):

山口, 浩; ヤマグチ, �. (n.d.). Studies on the structures and ion transport activities of transmembrane domains for a design of peptide channel : Studies on the structures and ion transport activities of transmembrane domains for a design of peptide channel; Studies on the structures and ion transport activities of transmembrane domains for a design of peptide channel. (Thesis). Saga University / 佐賀大学. Retrieved from http://portal.dl.saga-u.ac.jp/handle/123456789/8437

Note: this citation may be lacking information needed for this citation format:
No year of publication.
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

山口, 浩; ヤマグチ, ヒロシ. “Studies on the structures and ion transport activities of transmembrane domains for a design of peptide channel : Studies on the structures and ion transport activities of transmembrane domains for a design of peptide channel; Studies on the structures and ion transport activities of transmembrane domains for a design of peptide channel.” Thesis, Saga University / 佐賀大学. Accessed January 18, 2021. http://portal.dl.saga-u.ac.jp/handle/123456789/8437.

Note: this citation may be lacking information needed for this citation format:
No year of publication.
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

山口, 浩; ヤマグチ, ヒロシ. “Studies on the structures and ion transport activities of transmembrane domains for a design of peptide channel : Studies on the structures and ion transport activities of transmembrane domains for a design of peptide channel; Studies on the structures and ion transport activities of transmembrane domains for a design of peptide channel.” Web. 18 Jan 2021.

Note: this citation may be lacking information needed for this citation format:
No year of publication.

Vancouver:

山口, 浩; ヤマグチ �. Studies on the structures and ion transport activities of transmembrane domains for a design of peptide channel : Studies on the structures and ion transport activities of transmembrane domains for a design of peptide channel; Studies on the structures and ion transport activities of transmembrane domains for a design of peptide channel. [Internet] [Thesis]. Saga University / 佐賀大学; [cited 2021 Jan 18]. Available from: http://portal.dl.saga-u.ac.jp/handle/123456789/8437.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
No year of publication.

Council of Science Editors:

山口, 浩; ヤマグチ �. Studies on the structures and ion transport activities of transmembrane domains for a design of peptide channel : Studies on the structures and ion transport activities of transmembrane domains for a design of peptide channel; Studies on the structures and ion transport activities of transmembrane domains for a design of peptide channel. [Thesis]. Saga University / 佐賀大学; Available from: http://portal.dl.saga-u.ac.jp/handle/123456789/8437

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
No year of publication.

The Ohio State University

23. Conroy, Daniel William. Structural Studies of Biomolecules by Dynamic Nuclear Polarization Solid-State NMR Spectroscopy.

Degree: PhD, Chemistry, 2019, The Ohio State University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=osu1555428362333615

► Solid-state NMR (SSNMR) spectroscopy is an incredibly powerful tool for studying the structure and dynamics of biomolecules and large macromolecular complexes. SSNMR has no inherent… (more)

▼ Solid-state NMR (SSNMR) spectroscopy is an incredibly powerful tool for studying the structure and dynamics of biomolecules and large macromolecular complexes. SSNMR has no inherent size restriction and is useful in studying non-crystalline protein such as membrane-bound protein or amyloid fibrils, DNA-protein complexes, and large protein assemblies such as the HIV-capsid. Meanwhile, techniques such as solution-state NMR and x-ray crystallography are strictly limited by molecular size and sample condition, and thus cannot study large biomolecules or insoluble protein aggregates, respectively. The first protein at the center of this study is the human prion protein (PrP) and its formation into an insoluble amyloid fibril. The formation of this fibril leads to a deposition of an insoluble plaque on the central nervous system which leads to the development of the prion protein disease, known as Gerstmann-Straussler-Scheinker (GSS) disease. The second system-of-interest is the nucleosome core particle (NCP) which is a DNA-protein complex and is the building-block of chromatin and chromosomes. An individual NCP is composed of dsDNA wrapped around an octamer of histone protein, mimicking the natural phenomena of DNA storage and compaction in eukaryotic cells. SSNMR is uniquely qualified in studying these biological systems in depth to characterize the amyloid fibril propagation and disease-causing mechanism of prion protein, and to explore nucleic acid base-pairing behavior at DNA-protein interfaces and the important interactions therein.Dynamic nuclear polarization (DNP) is a hyperpolarization technique for NMR spectroscopy which dramatically increases the overall sensitivity of these experiments. In DNP-SSNMR, hyperpolarization is achieved by applying microwave irradiation to free electrons, often in the form of stable-radicals, within a static magnetic field and inducing a polarization transfer to neighboring nuclear spins, especially spin-½ protons. These elevated levels of magnetization in protons can be passed on to other NMR-active nuclei, in particular carbon-13 (13C) and nitrogen-15 (15N), using standard SSNMR experimental protocols. This process can generate NMR signal enhancements ranging from 20 to 200 and may be generally applied to small molecules, solid-phase materials and biological samples. DNP offers significant time savings when performing complex SSNMR experiments and opens the door for studying dilute species, weak interactions, or minor populations which are typically below the threshold of standard NMR spectroscopy.The primary goal of this dissertation is to develop strategies to study the structures of prion protein amyloid fibrils and dsDNA by expanding on current DNP-SSNMR methodologies, as well as introducing a novel class of biradicals. First, new polarizing agents, peptide-based biradicals are synthesized, purified, characterized and are successfully employed to study small molecules, crystalline protein, and amyloid protein by DNP-SSNMR. These dinitroxides include TOAC-TOAC (TT), TOAC-Ser-TOAC… Advisors/Committee Members: Jaroniec, Christopher (Advisor).

Subjects/Keywords: Chemistry; Solid-State NMR Spectroscopy; Dynamic Nuclear Polarization; Dinitroxide; Biradical; Polarizing Agent; Solid-Phase Peptide Synthesis; TOAC; DNA; Hoogsteen Base-Pairing; Nucleosome Core Particle; Amyloid Fibril; Prion Protein

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APA (6^th Edition):

Conroy, D. W. (2019). Structural Studies of Biomolecules by Dynamic Nuclear Polarization Solid-State NMR Spectroscopy. (Doctoral Dissertation). The Ohio State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=osu1555428362333615

Chicago Manual of Style (16^th Edition):

Conroy, Daniel William. “Structural Studies of Biomolecules by Dynamic Nuclear Polarization Solid-State NMR Spectroscopy.” 2019. Doctoral Dissertation, The Ohio State University. Accessed January 18, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=osu1555428362333615.

MLA Handbook (7^th Edition):

Conroy, Daniel William. “Structural Studies of Biomolecules by Dynamic Nuclear Polarization Solid-State NMR Spectroscopy.” 2019. Web. 18 Jan 2021.

Vancouver:

Conroy DW. Structural Studies of Biomolecules by Dynamic Nuclear Polarization Solid-State NMR Spectroscopy. [Internet] [Doctoral dissertation]. The Ohio State University; 2019. [cited 2021 Jan 18]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1555428362333615.

Council of Science Editors:

Conroy DW. Structural Studies of Biomolecules by Dynamic Nuclear Polarization Solid-State NMR Spectroscopy. [Doctoral Dissertation]. The Ohio State University; 2019. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1555428362333615

24. Gresika, Alexandra. Nouveaux synthons contraints de type α-amino acides et PNA en vue de l’élaboration de bio-foldamères : New constrained amino acids and peptide nucleic acid building blocks for the construction of bio-polymers.

Degree: Docteur es, Chimie, 2018, Université Côte d'Azur (ComUE)

URL: http://www.theses.fr/2018AZUR4104

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Le but de cette thèse a concerné le développement de deux types de structures oligomériques contraintes, basées soit sur des dérivés non naturels d’acides aminés… (more)

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Le but de cette thèse a concerné le développement de deux types de structures oligomériques contraintes, basées soit sur des dérivés non naturels d’acides aminés cycliques, soit sur des dimères cycliques de Peptide Nucleic Acids (PNA), en vue d’analyser leur tendance à adopter des conformations pré-organisées, imitant les conformations actives des protéines ou des acides nucléiques. La première partie de cette thèse a trait à l’élaboration de synthons clefs dérivant d’acides α-aminés cycliques non naturels (acides 4-oxopipécoliques 6-substitués), à leur utilisation potentielle dans la synthèse de peptidomimétiques contraints, homogènes et hétérogènes, ainsi qu’à leurs limites dans cette utilisation. Dans le cadre de ce travail, une nouvelle voie de synthèse permettant d’accéder à des résidus N-protégés d'acides 4-oxo-pipécoliques 6substitués a été mise au point. La deuxième partie de cette thèse concerne l’élaboration de dimères d’α-PNA (di-α-PNA) cycliques, dans lesquels les chaînes latérales de deux monomères d’α-PNA consécutifs sont «agrafées» via un pont lactame. Une stratégie de synthèse a tout d'abord été développée en phase liquide, puis appliquée à la synthèse en phase solide de di-α-PNA «agrafés» modèles, incorporant des bases nucléiques thymine.

The purpose of this thesis concerned the development of two kinds of constrained oligomeric structures, based either on unnatural cyclic α-amino acids derivatives or on cyclic Peptide Nucleic Acid (PNA) dimers, in view of analyzing their propensity to adopt pre-organized conformations mimicking the active conformations of proteins or nucleic acids. The first part of this thesis reports on the elaboration of new cyclic αamino-acids (6-substituted 4-oxopipecolic acids) building blocks, their potential use in the synthesis of constrained homogenous and heterogeneous peptidomimetics as well as their limitations in this use. As part of this work, a new methodology has been developed for the synthesis of N-protected 6-substituted 4-oxo-pipecolic acids residues. The second part of this thesis reports on the elaboration of constrained α-PNA dimers (di-α-PNA), in which the side-chains of two consecutive α-PNA monomers are “stapled” via a lactam bridge. A synthetic orthogonal strategy has been first developed in liquid-phase then applied to the solid-phase synthesis of models “stapled” di-α PNA incorporating thymine nucleobases.

Advisors/Committee Members: Patino, Nadia (thesis director).

Subjects/Keywords: Foldamères; Peptidomimétiques contraints; PNA contraints; Analogues d'acides nucléiques; PNA agrafés; Structures pré-organisées; Synthèse peptidique; Phase-solide; Foldamers; Constrained peptidomimetics; Constrained Peptide Nucleic Acids; Nucleic Acid analogs; Stapled Peptide Nucleic Acids; Pre-organized structures; Peptide synthesis; Solid-phase

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Gresika, A. (2018). Nouveaux synthons contraints de type α-amino acides et PNA en vue de l’élaboration de bio-foldamères : New constrained amino acids and peptide nucleic acid building blocks for the construction of bio-polymers. (Doctoral Dissertation). Université Côte d'Azur (ComUE). Retrieved from http://www.theses.fr/2018AZUR4104

Chicago Manual of Style (16^th Edition):

Gresika, Alexandra. “Nouveaux synthons contraints de type α-amino acides et PNA en vue de l’élaboration de bio-foldamères : New constrained amino acids and peptide nucleic acid building blocks for the construction of bio-polymers.” 2018. Doctoral Dissertation, Université Côte d'Azur (ComUE). Accessed January 18, 2021. http://www.theses.fr/2018AZUR4104.

MLA Handbook (7^th Edition):

Gresika, Alexandra. “Nouveaux synthons contraints de type α-amino acides et PNA en vue de l’élaboration de bio-foldamères : New constrained amino acids and peptide nucleic acid building blocks for the construction of bio-polymers.” 2018. Web. 18 Jan 2021.

Vancouver:

Gresika A. Nouveaux synthons contraints de type α-amino acides et PNA en vue de l’élaboration de bio-foldamères : New constrained amino acids and peptide nucleic acid building blocks for the construction of bio-polymers. [Internet] [Doctoral dissertation]. Université Côte d'Azur (ComUE); 2018. [cited 2021 Jan 18]. Available from: http://www.theses.fr/2018AZUR4104.

Council of Science Editors:

Gresika A. Nouveaux synthons contraints de type α-amino acides et PNA en vue de l’élaboration de bio-foldamères : New constrained amino acids and peptide nucleic acid building blocks for the construction of bio-polymers. [Doctoral Dissertation]. Université Côte d'Azur (ComUE); 2018. Available from: http://www.theses.fr/2018AZUR4104

Mahatma Gandhi University

25. Sebastian, Saju M. Microwave assisted reactions using polyvinylpyrrolidone supported reagents;.

Degree: Microwave, 2014, Mahatma Gandhi University

URL: http://shodhganga.inflibnet.ac.in/handle/10603/19769

►

Polyvinylpyrrolidone-bromine complex was synthesized and used as a versatile and efficient polymeric reagent for the oxidation of alcohols, dihalogenation of unsaturated compounds, and#61537;-halogenation of ketones,… (more)

Subjects/Keywords: Microwave assisted reactions; Polyvinylpyrrolidone; Solid phase synthesis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sebastian, S. M. (2014). Microwave assisted reactions using polyvinylpyrrolidone supported reagents;. (Thesis). Mahatma Gandhi University. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/19769

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Sebastian, Saju M. “Microwave assisted reactions using polyvinylpyrrolidone supported reagents;.” 2014. Thesis, Mahatma Gandhi University. Accessed January 18, 2021. http://shodhganga.inflibnet.ac.in/handle/10603/19769.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Sebastian, Saju M. “Microwave assisted reactions using polyvinylpyrrolidone supported reagents;.” 2014. Web. 18 Jan 2021.

Vancouver:

Sebastian SM. Microwave assisted reactions using polyvinylpyrrolidone supported reagents;. [Internet] [Thesis]. Mahatma Gandhi University; 2014. [cited 2021 Jan 18]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/19769.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Sebastian SM. Microwave assisted reactions using polyvinylpyrrolidone supported reagents;. [Thesis]. Mahatma Gandhi University; 2014. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/19769

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Illinois – Urbana-Champaign

26. Mukherjee, Subha. Mechanistic and synthetic studies on the prochlorosin and cytolysin families of lanthipeptides.

Degree: PhD, Chemistry, 2015, University of Illinois – Urbana-Champaign

URL: http://hdl.handle.net/2142/89174

► Peptides are an attractive class of therapeutics, occupying a niche between small molecules and biologics. Research in the van der Donk lab focuses on lanthipeptides,… (more)

▼ Peptides are an attractive class of therapeutics, occupying a niche between small molecules and biologics. Research in the van der Donk lab focuses on lanthipeptides, a class of ribosomally synthesized and post-translationally modified peptides (RiPPs) that commonly feature antibacterial activity and contain the characteristic thioether residues lanthionine (Lan) and methyllanthionine (MeLan). Installation of thioether crosslinks in lanthipeptide biosynthesis is carried out by designated synthetases and involves dehydration of Ser/Thr residues and cyclization via Michael-type addition. The remarkably broad substrate scope of the synthetase ProcM inspired us to explore its mechanism in detail (chapter 2). My studies on ProcM revealed the directionality of dehydration, the order of cyclization, and that, despite the impressive substrate scope, none of the cyclizations are non-enzymatic. In collaboration, we established the irreversibility of the Michael-type addition and proposed that the topology of the thioether rings is under kinetic control. Solid phase peptide synthesis (SPPS) was used to generate the substrates to study ProcM, and is also a flexibile tool to access non-native lanthipeptide analogues. Interestingly, a lanthipeptide, cytolysin S (CylLS”), exhibited cytolytic activity in synergy with cytolysin L (CylLL”). Given that a thioether crosslink in CylLS” has an unusual LL-stereochemistry, the synthesis of a diastereomer of CylLS” with the more common DL-stereochemistry was achieved by SPPS (chapter 3). We probed whether the cytolytic activity depended on the LL-stereochemistry observed in CylLS”. Surprisingly, the unusual LL-stereochemistry was found to be important for the antibacterial activity, but not necessary for the hemolytic activity of CylLS”. I have also synthesized another hydrophobic lanthipeptide analogue, the portion of microbisporicin that contains the A and B ring (chapter 4). We established that this motif is not recognized by the halogenase MibH, and that the C terminus of microbisporicin is necessary for the chlorination by MibH. During my graduate studies, I had the opportunity to collaborate in a different area of research in our laboratory, the phosphonates. My efforts in the syntheses of various substrates and intermediates were instrumental in elucidating the biosynthetic pathways of dehydrophos, fosfazinomycin, and rhizocticin (chapter 5). Advisors/Committee Members: van der Donk, Wilfred Adrianus (advisor), van der Donk, Wilfred Adrianus (Committee Chair), Burke, Martin D. (committee member), Metcalf, William W. (committee member), Zhao, Huimin (committee member).

Subjects/Keywords: ribosomally synthesized and post-translationally modified peptides (RiPPs); Lanthipeptide; Lanthionine; antibiotics; antimicrobial resistance; prochlorosin; ProcM; Solid phase peptide synthesis (SPPS); lanthipeptide synthesis; hydrophobic peptide synthesis; dehydroalanine; Michael-type addition; non-enzymatic cyclization; cytolysin; microbisporicin; phosphonate; dehydrophos; fosfazinomycin; rhizocticin

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mukherjee, S. (2015). Mechanistic and synthetic studies on the prochlorosin and cytolysin families of lanthipeptides. (Doctoral Dissertation). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/89174

Chicago Manual of Style (16^th Edition):

Mukherjee, Subha. “Mechanistic and synthetic studies on the prochlorosin and cytolysin families of lanthipeptides.” 2015. Doctoral Dissertation, University of Illinois – Urbana-Champaign. Accessed January 18, 2021. http://hdl.handle.net/2142/89174.

MLA Handbook (7^th Edition):

Mukherjee, Subha. “Mechanistic and synthetic studies on the prochlorosin and cytolysin families of lanthipeptides.” 2015. Web. 18 Jan 2021.

Vancouver:

Mukherjee S. Mechanistic and synthetic studies on the prochlorosin and cytolysin families of lanthipeptides. [Internet] [Doctoral dissertation]. University of Illinois – Urbana-Champaign; 2015. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/2142/89174.

Council of Science Editors:

Mukherjee S. Mechanistic and synthetic studies on the prochlorosin and cytolysin families of lanthipeptides. [Doctoral Dissertation]. University of Illinois – Urbana-Champaign; 2015. Available from: http://hdl.handle.net/2142/89174

27. Titirici, Maria-Magdalena. Synthesis and evaluation of novel formats in molecular imprinting.

Degree: 2005, Universität Dortmund

URL: http://hdl.handle.net/2003/20168

Subjects/Keywords: Grafting; MIPS; Molecular recognition; Peptide recognition; Solid phase synthesis; 540

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❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Titirici, M. (2005). Synthesis and evaluation of novel formats in molecular imprinting. (Thesis). Universität Dortmund. Retrieved from http://hdl.handle.net/2003/20168

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Titirici, Maria-Magdalena. “Synthesis and evaluation of novel formats in molecular imprinting.” 2005. Thesis, Universität Dortmund. Accessed January 18, 2021. http://hdl.handle.net/2003/20168.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Titirici, Maria-Magdalena. “Synthesis and evaluation of novel formats in molecular imprinting.” 2005. Web. 18 Jan 2021.

Vancouver:

Titirici M. Synthesis and evaluation of novel formats in molecular imprinting. [Internet] [Thesis]. Universität Dortmund; 2005. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/2003/20168.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Titirici M. Synthesis and evaluation of novel formats in molecular imprinting. [Thesis]. Universität Dortmund; 2005. Available from: http://hdl.handle.net/2003/20168

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

28. Perdikari, Aikaterini. Παράγωγα ινσουλίνης.

Degree: 2017, University of Patras; Πανεπιστήμιο Πατρών

URL: http://hdl.handle.net/10442/hedi/41454

►

Insulin is a peptide hormone produced by the beta cells of the pancreas and plays a role in the metabolism of the body's sugars. The… (more)

▼

Insulin is a peptide hormone produced by the beta cells of the pancreas and plays a role in the metabolism of the body's sugars. The insulin derivatives have been designed to maintain structural features necessary for biological activity of the molecule. Derivatives of this are related to a lower risk of hypoglycemia, lower levels of postprandial rise of glucose, better quality of life and some of them provide less weight gain, which is the inevitable result of replacement of insulin. The purpose of this thesis was the synthesis of insulin derivatives in order to optimize the metabolic stability of these molecules; to permit their prolonged activity during their use as pharmaceuticals. The hydrophobicity of the A chain (InsA), which is very difficult to be isolated, and the formation of three disulfide bonds, which are difficult to form correctly in high yield, constitutes a challenge for the chemical synthesis of insulin. These derivatives consist of two polypeptide chains, the H-InsA(1-20)-OH or H-LanA(1-21)-OH and H-[desThr30InsB (1-29)]-OH. The two chains are linked together by a peptide bond through amino acid Fmoc-Glu-OtBu, while the amino acid Gly21 is further added to the derivative with chain LanA(1-21), to test its biological activity. The tertiary structure of derivatives is stabilized by two disulfide bonds between the two chains, while one intramolecular coexists in A chain. For the synthesis are used the 4-methylbenzydrylbromide resin (MBH) and 2-chlorotritylchloride resin (CLTR). Because of the insolubility of these molecules, their composition was attempted with fragment condensation in liquid phase and chemical ligation. The completion of the synthesis was followed by either random oxidation or regioselective formation of disulfide bonds of molecules, in order to obtain the final derivatives.

Η ινσουλίνη είναι μία πεπτιδική ορμόνη που παράγεται από τα β-κύτταρα του παγκρέατος και παίζει ρόλο στο μεταβολισμό των σακχάρων του οργανισμού. Tα παράγωγα της ινσουλίνης έχουν σχεδιασθεί ώστε να διατηρούν δομικά χαρακτηριστικά απαραίτητα για τη βιολογική δράση του μορίου. Τα παράγωγα αυτής σχετίζονται με χαμηλότερο κίνδυνο υπογλυκαιμίας, χαμηλότερα επίπεδα της μεταγευματικής αύξησης της γλυκόζης, καλύτερη ποιότητα ζωής και κάποια εξ αυτών προσφέρουν μικρότερη αύξηση του σωματικού βάρους, η οποία αποτελεί αναπόφευκτη συνέπεια αντικατάστασης της ινσουλίνης. Στόχος της παρούσας Διδακτορικής διατριβής ήταν η σύνθεση παραγώγων ινσουλίνης με σκοπό την βελτιστοποίηση της μεταβολικής σταθερότητας των μορίων αυτών, ώστε να είναι δυνατή η παρατεταμένη δράση τους κατά τη χρήση τους ως πιθανά φαρμακευτικά σκευάσματα. Η υδροφοβικότητα της αλυσίδας Α (InsA), η οποία είναι πολύ δύσκολο να απομονωθεί, καθώς και ο σχηματισμός των τριών δισουλφιδικών δεσμών, οι οποίοι είναι δύσκολο να σχηματιστούν σωστά σε υψηλή απόδοση, αποτελούν πρόκληση για τη χημική σύνθεση της ινσουλίνης και των παραγώγων της. Τα παράγωγα στην παρούσα μελέτη, αποτελούνται από δύο πολυπεπτιδικές αλυσίδες, την Η-ΙnsA(1-20)-ΟΗ ή Η-LanA(1-21)-ΟΗ και την…

Subjects/Keywords: Πεπτιδική σύνθεση σε στερεά φάση; 4-μεθυλoβενζυδρυλβρωμίδιο ρητίνη; Πυρογλουταμινικό οξύ; Ινσουλίνη; Παράγωγα ινσουλίνης; Solid phase peptide synthesis; 4-methylbenzhydrylbromide resin; Pyroglutamic acid; Insulin; Insulin derivatives

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Perdikari, A. (2017). Παράγωγα ινσουλίνης. (Thesis). University of Patras; Πανεπιστήμιο Πατρών. Retrieved from http://hdl.handle.net/10442/hedi/41454

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Perdikari, Aikaterini. “Παράγωγα ινσουλίνης.” 2017. Thesis, University of Patras; Πανεπιστήμιο Πατρών. Accessed January 18, 2021. http://hdl.handle.net/10442/hedi/41454.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Perdikari, Aikaterini. “Παράγωγα ινσουλίνης.” 2017. Web. 18 Jan 2021.

Vancouver:

Perdikari A. Παράγωγα ινσουλίνης. [Internet] [Thesis]. University of Patras; Πανεπιστήμιο Πατρών; 2017. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/10442/hedi/41454.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Perdikari A. Παράγωγα ινσουλίνης. [Thesis]. University of Patras; Πανεπιστήμιο Πατρών; 2017. Available from: http://hdl.handle.net/10442/hedi/41454

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

29. Ludolph, Björn. Festphasensynthese lipidierter Peptide und Benzodiazepindione.

Degree: 2004, Technische Universität Dortmund

URL: http://dx.doi.org/10.17877/DE290R-14873

► Lipopeptide sind wertvolle Werkzeuge zur Aufklärung biologischer Fragestellungen im molekularen Detail. In dieser Arbeit wurden effiziente Festphasensynthese-Methoden zur Darstellung verschiedener Lipopeptide entwickelt. Schlüsselelement in der… (more)

Subjects/Keywords: Lipopeptide; Ras-Peptide; Festphasensynthese; Benzodiazepindione; lipopeptids; Ras-peptids; solid phase synthesis; benzodiazepinedions; 540

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ludolph, B. (2004). Festphasensynthese lipidierter Peptide und Benzodiazepindione. (Doctoral Dissertation). Technische Universität Dortmund. Retrieved from http://dx.doi.org/10.17877/DE290R-14873

Chicago Manual of Style (16^th Edition):

Ludolph, Björn. “Festphasensynthese lipidierter Peptide und Benzodiazepindione.” 2004. Doctoral Dissertation, Technische Universität Dortmund. Accessed January 18, 2021. http://dx.doi.org/10.17877/DE290R-14873.

MLA Handbook (7^th Edition):

Ludolph, Björn. “Festphasensynthese lipidierter Peptide und Benzodiazepindione.” 2004. Web. 18 Jan 2021.

Vancouver:

Ludolph B. Festphasensynthese lipidierter Peptide und Benzodiazepindione. [Internet] [Doctoral dissertation]. Technische Universität Dortmund; 2004. [cited 2021 Jan 18]. Available from: http://dx.doi.org/10.17877/DE290R-14873.

Council of Science Editors:

Ludolph B. Festphasensynthese lipidierter Peptide und Benzodiazepindione. [Doctoral Dissertation]. Technische Universität Dortmund; 2004. Available from: http://dx.doi.org/10.17877/DE290R-14873

30. Titirici, Maria-Magdalena. Synthesis and evaluation of novel formats in molecular imprinting.

Degree: 2005, Technische Universität Dortmund

URL: http://dx.doi.org/10.17877/DE290R-12596

Subjects/Keywords: Molecular recognition; MIPS; Grafting; Solid phase synthesis; Peptide recognition; 540

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Titirici, M. (2005). Synthesis and evaluation of novel formats in molecular imprinting. (Doctoral Dissertation). Technische Universität Dortmund. Retrieved from http://dx.doi.org/10.17877/DE290R-12596

Chicago Manual of Style (16^th Edition):

Titirici, Maria-Magdalena. “Synthesis and evaluation of novel formats in molecular imprinting.” 2005. Doctoral Dissertation, Technische Universität Dortmund. Accessed January 18, 2021. http://dx.doi.org/10.17877/DE290R-12596.

MLA Handbook (7^th Edition):

Titirici, Maria-Magdalena. “Synthesis and evaluation of novel formats in molecular imprinting.” 2005. Web. 18 Jan 2021.

Vancouver:

Titirici M. Synthesis and evaluation of novel formats in molecular imprinting. [Internet] [Doctoral dissertation]. Technische Universität Dortmund; 2005. [cited 2021 Jan 18]. Available from: http://dx.doi.org/10.17877/DE290R-12596.

Council of Science Editors:

Titirici M. Synthesis and evaluation of novel formats in molecular imprinting. [Doctoral Dissertation]. Technische Universität Dortmund; 2005. Available from: http://dx.doi.org/10.17877/DE290R-12596

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