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You searched for subject:(Sister chromatid intertwines). One record found.

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1. L.F. Massari. Complete resolution of sister chromatid intertwines requires the Polo-like kinase Cdc5 and the phosphatase Cdc14 in budding yeast.

Degree: 2018, Università degli Studi di Milano

During mitosis the newly replicated genetic material, organized in sister chromatids, is equally subdivided into the daughter cells through a fine-regulated process called chromosome segregation. Sister chromatids are held together and identified as sisters by cohesin. At the metaphase-to-anaphase transition, when all chromatids are correctly attached to the spindle, cohesin is cleaved and chromosome segregation initiates. Beside cohesin, all linkages between sister chromatids need to be removed to allow for their complete separation. Additional linkages include DNA linkages (or sister chromatid intertwines, SCIs), such as recombination intermediates and DNA catenanes. In Saccharomyces cerevisiae a mutant that lacks the activities of the Polo-like kinase Cdc5 and the phosphatase Cdc14, two major mitotic regulators, has been identified that proved to be particularly suitable for studying SCIs that persist in mitosis. The cdc5 cdc14 double mutant arrests with short and stable mitotic spindles and unseparated nuclei, despite having cleaved cohesin. In addition to having a spindle elongation defect, these cells are also impaired in the resolution of cohesin-independent linkages between chromatids. We found that these linkages mostly consist of DNA catenanes, that persist in cdc5 cdc14 cells at their terminal arrest and that are sufficient to counteract spindle elongation. Our results suggest that Cdc5 is required for their resolution. This finding, together with the knowledge that Cdc5 promotes Cdc14 activation and that both proteins are essential for spindle elongation and mitotic exit, allows us to speculate that they coordinate different aspects of chromosome segregation to guarantee genome integrity throughout mitosis. Advisors/Committee Members: external Advisor: F. Uhlmann, internal advisor: V. Costanzo, supervisor: R. Visintin.

Subjects/Keywords: Mitosis; Chromosome segregation; Genome stability; Sister chromatid intertwines; Cdc5; Cdc14; Top2; condensin; Yen1; Yeast; Settore BIO/11 - Biologia Molecolare

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APA (6th Edition):

Massari, L. (2018). Complete resolution of sister chromatid intertwines requires the Polo-like kinase Cdc5 and the phosphatase Cdc14 in budding yeast. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/556680

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Massari, L.F.. “Complete resolution of sister chromatid intertwines requires the Polo-like kinase Cdc5 and the phosphatase Cdc14 in budding yeast.” 2018. Thesis, Università degli Studi di Milano. Accessed November 17, 2019. http://hdl.handle.net/2434/556680.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Massari, L.F.. “Complete resolution of sister chromatid intertwines requires the Polo-like kinase Cdc5 and the phosphatase Cdc14 in budding yeast.” 2018. Web. 17 Nov 2019.

Vancouver:

Massari L. Complete resolution of sister chromatid intertwines requires the Polo-like kinase Cdc5 and the phosphatase Cdc14 in budding yeast. [Internet] [Thesis]. Università degli Studi di Milano; 2018. [cited 2019 Nov 17]. Available from: http://hdl.handle.net/2434/556680.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Massari L. Complete resolution of sister chromatid intertwines requires the Polo-like kinase Cdc5 and the phosphatase Cdc14 in budding yeast. [Thesis]. Università degli Studi di Milano; 2018. Available from: http://hdl.handle.net/2434/556680

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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