subject:(Settore VET 01 Anatomia degli Animali Domestici)

1. A.A.S.M. Aufy. PROTEIN PURIFICATION FROM GASTROPODS.

Degree: 2011, Università degli Studi di Milano

► Gastropods are a class of invertebrates within the mollusks, commonly known as snails and slugs. Recently, scientific interest is directed on snails as pollution indicators,… (more)

▼ Gastropods are a class of invertebrates within the mollusks, commonly known as snails and slugs. Recently, scientific interest is directed on snails as pollution indicators, as destroyers of crops, but also as parasites carriers. Furthermore, some species of snails are considered as an important human food source in countries like France and Australia. In scientific research, snails are used as model animals especially in molecular biology and immunology. Some snails secrete purple matter with anti-cancer property; the snails use it to protect their eggs and scientists hope to create with it a weapon against breast cancer. In the light of this, the aim of the present work was to purify and characterize exoglycosidases, i.e. sugars-hydrolyzing enzymes, extracted from different species of snails and to study the glycosylation pattern of their tissues by using lectins as glycoprotein-specific antibodies. Purification work started with the screening of 7 different exoglycosidases (α-fucosidase, β-xylosidase, α-mannosidase, β-mannosidase, α-glucosidase, β-glucosidase, β- galactosidase and β-N-acetylglucosaminidase) in 8 different species of snails (Arion lusitanicus, Biomphalaria glabrata, Achatina fulica, Limax maximus, Cepaea hortensis, Lymnea stagnalis, Arianta arbustorum, Planorbarius corneus and egg from Achatina fulica). In order to create a purification scheme, many trials have been performed on the chosen enzyme, i.e. β-galactosidase from Arion lusitanicus. Ten snails were carefully washed after complete removal of the abdomen content to eliminate other sources of the enzyme. Snails were then homogenized and protein was precipitated by 1.2 M ammonium sulfate under cooling centrifugation. Then the precipitate was fractionated by the column hydrophobic interaction chromatography. Fractions exhibiting high enzyme activity were pooled, concentrated, desalted by ultrafiltration and then applied to affi gel blue. As the use of affi gel blue resulted in no binding between the enzyme and the gel beads, the unbound fractions were directly applied to anion exchange chromatography. As this procedure behaved as the same way of affi gel blue, the unbound fractions were pooled, concentrated to less than 2 ml and applied to size exclusion chromatography. Size exclusion chromatography exhibited high resolution purification, because a narrow peak was obtained, after testing the eluate for the enzyme activity. The fractions within this narrow peak were then applied to cation exchange chromatography, resulting in two new very sharp peaks. Fractions of the second peak from cation exchange chromatography were exposed to SDS-PAGE that indicated high quality purification, because just two proteins’ bands appeared. Finally, β-galactosidase specific affinity was the last purification step, where four different buffers were used (50mM sodium citrate pH 4.6, 1M NaCl in50 mM sodium citrate pH 4.6, 50 mM Tris/HCl pH 7.5 and 1M NaCl in 50 mM Tris/HCl pH 7.5) and the enzyme was eluted only with 50 mM Tris/HCl pH 7.5 buffer. … Advisors/Committee Members: tutor: Fabia Rosi, co-tutor: Erika Staudacher, coordinatore: Graziano Zocchi, ROSI, FABIA, ZOCCHI, GRAZIANO.

Subjects/Keywords: Settore VET/01 - Anatomia degli Animali Domestici

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Aufy, A. (2011). PROTEIN PURIFICATION FROM GASTROPODS. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/152912

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Aufy, A.A.S.M.. “PROTEIN PURIFICATION FROM GASTROPODS.” 2011. Thesis, Università degli Studi di Milano. Accessed January 22, 2021. http://hdl.handle.net/2434/152912.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Aufy, A.A.S.M.. “PROTEIN PURIFICATION FROM GASTROPODS.” 2011. Web. 22 Jan 2021.

Vancouver:

Aufy A. PROTEIN PURIFICATION FROM GASTROPODS. [Internet] [Thesis]. Università degli Studi di Milano; 2011. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/2434/152912.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Aufy A. PROTEIN PURIFICATION FROM GASTROPODS. [Thesis]. Università degli Studi di Milano; 2011. Available from: http://hdl.handle.net/2434/152912

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

2. G. Pennarossa. CENTROSOME BIOGENESIS AND ADAPTIVE RESPONSE IN MAMMAL PARTHENOGENETIC CELLS.

Degree: 2012, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/169555

► Human parthenotes have been proposed as a source of embryonic stem cells despite the high incidence of aneuploidy described in parthenotes of most mammalian species.… (more)

Subjects/Keywords: Settore VET/01 - Anatomia degli Animali Domestici

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Pennarossa, G. (2012). CENTROSOME BIOGENESIS AND ADAPTIVE RESPONSE IN MAMMAL PARTHENOGENETIC CELLS. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/169555

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Pennarossa, G.. “CENTROSOME BIOGENESIS AND ADAPTIVE RESPONSE IN MAMMAL PARTHENOGENETIC CELLS.” 2012. Thesis, Università degli Studi di Milano. Accessed January 22, 2021. http://hdl.handle.net/2434/169555.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Pennarossa, G.. “CENTROSOME BIOGENESIS AND ADAPTIVE RESPONSE IN MAMMAL PARTHENOGENETIC CELLS.” 2012. Web. 22 Jan 2021.

Vancouver:

Pennarossa G. CENTROSOME BIOGENESIS AND ADAPTIVE RESPONSE IN MAMMAL PARTHENOGENETIC CELLS. [Internet] [Thesis]. Università degli Studi di Milano; 2012. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/2434/169555.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Pennarossa G. CENTROSOME BIOGENESIS AND ADAPTIVE RESPONSE IN MAMMAL PARTHENOGENETIC CELLS. [Thesis]. Università degli Studi di Milano; 2012. Available from: http://hdl.handle.net/2434/169555

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

3. R. Pasquariello. MOLECULAR CHARACTERIZATION OF FACTORS WHICH CAN INFLUENCE THE REPRODUCTIVE SUCCESS OF CATTLE.

Degree: 2015, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/335298

► My PhD project addressed the paternal and maternal contribution associated with correct early embryonic development, focusing on microRNAs (miRNAs, short non-coding RNAs regulating gene expression… (more)

Subjects/Keywords: Settore VET/01 - Anatomia degli Animali Domestici

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Pasquariello, R. (2015). MOLECULAR CHARACTERIZATION OF FACTORS WHICH CAN INFLUENCE THE REPRODUCTIVE SUCCESS OF CATTLE. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/335298

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Pasquariello, R.. “MOLECULAR CHARACTERIZATION OF FACTORS WHICH CAN INFLUENCE THE REPRODUCTIVE SUCCESS OF CATTLE.” 2015. Thesis, Università degli Studi di Milano. Accessed January 22, 2021. http://hdl.handle.net/2434/335298.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Pasquariello, R.. “MOLECULAR CHARACTERIZATION OF FACTORS WHICH CAN INFLUENCE THE REPRODUCTIVE SUCCESS OF CATTLE.” 2015. Web. 22 Jan 2021.

Vancouver:

Pasquariello R. MOLECULAR CHARACTERIZATION OF FACTORS WHICH CAN INFLUENCE THE REPRODUCTIVE SUCCESS OF CATTLE. [Internet] [Thesis]. Università degli Studi di Milano; 2015. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/2434/335298.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Pasquariello R. MOLECULAR CHARACTERIZATION OF FACTORS WHICH CAN INFLUENCE THE REPRODUCTIVE SUCCESS OF CATTLE. [Thesis]. Università degli Studi di Milano; 2015. Available from: http://hdl.handle.net/2434/335298

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

4. A. Zenobi. CHARACTERIZATION OF MOLECULAR MECHANISMS DRIVING EPIGENETIC CONVERSION AND PHENOTYPE SWITCH OF FIBROBLASTS INTO INSULIN SECRETING CELLS.

Degree: 2018, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/548193

►

La conversione epigenetica è una tecnica promettente che consente ad una cellula somatica matura di passare ad un fenotipo funzionale diverso ed alternativo rispetto a… (more)

▼

La conversione epigenetica è una tecnica promettente che consente ad una cellula somatica matura di passare ad un fenotipo funzionale diverso ed alternativo rispetto a quello di origine. Questo risultato viene perseguito senza alcuna modificazione transgenica e senza l'acquisizione di uno stato di pluripotenza stabile e irreversibile, caratteristiche che rendono questo approccio molto prezioso per la medicina rigenerativa. Il protocollo di conversione epigenetica è robusto, riproducibile e assicura una buona efficienza ed il conseguimento di un fenotipo funzionale. Tuttavia, le cellule ottenute non sono completamente mature e differenziate ed è necessario identificare le migliori condizioni per realizzare uno “scale-up” che permetta l’applicazione in studi preclinici. Lo scopo del presente progetto di dottorato è stato quello di individuare condizioni di coltura fisiologiche, limitando le differenze tra l’ambiente in vitro e quello in vivo, al fine di aumentare l'efficienza del processo di differenziamento. Più precisamente, sono state testate concentrazioni fisiologiche di ossigeno e di glucosio per poter valutare l’efficienza di conversione cellulare nei diversi ambienti. Parallelamente, è stato valutato l'uso di sistemi di coltura tridimensionale (3D), con lo scopo di studiare il loro impatto sull’efficienza di conversione e sull'acquisizione di un fenotipo funzionale e maturo. I dati ottenuti suggeriscono che il “background genetico” ha un effetto significativo sulla risposta cellulare alle diverse condizioni di ossigeno durante il processo di differenziamento. D’altro canto, l'efficienza di conversione è risultata strettamente dipendente dalle concentrazioni di glucosio utilizzate durante l’isolamento delle cellule dal tessuto di origine. Inoltre, l’utilizzo di sistemi di coltura 3D, che riflettono la rigidità e l’elasticità proprie dell'organo in vivo, ha dimostrato un effetto positivo per l'acquisizione di un fenotipo pancreatico maturo, tipico delle cellule terminalmente differenziate. Infine, le informazioni molecolari ottenute dagli esperimenti di “genome editing” (ancora in corso) dovrebbero ulteriormente chiarire e corroborare i dati ottenuti. Complessivamente, i risultati di questa tesi possono fornire informazioni utili sia per la comprensione dei meccanismi di base che regolano la crescita e il differenziamento cellulare, così come per la messa a punto di un protocollo di “scale-up” da utilizzare nella realizzazione di studi preclinici finalizzati alla medicina rigenerativa del diabete.

Epigenetic conversion is a powerful technique that allows a mature somatic cell to switch into a different and alternative functional phenotype. The result is acquired without any transgenic modification, nor the acquisition of a stable and irreversible pluripotent state, making this approach very valuable for regenerative medicine. The protocol is robust, reproducible and ensures good functional efficiency, however, cells obtained are not completely mature and the optimal scale up conditions are needed for clinical…

Advisors/Committee Members: tutor: T.Brevini, phd coordinator: F. Gandolfi, GANDOLFI, FULVIO.

Subjects/Keywords: Settore VET/01 - Anatomia degli Animali Domestici

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Zenobi, A. (2018). CHARACTERIZATION OF MOLECULAR MECHANISMS DRIVING EPIGENETIC CONVERSION AND PHENOTYPE SWITCH OF FIBROBLASTS INTO INSULIN SECRETING CELLS. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/548193

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Zenobi, A.. “CHARACTERIZATION OF MOLECULAR MECHANISMS DRIVING EPIGENETIC CONVERSION AND PHENOTYPE SWITCH OF FIBROBLASTS INTO INSULIN SECRETING CELLS.” 2018. Thesis, Università degli Studi di Milano. Accessed January 22, 2021. http://hdl.handle.net/2434/548193.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Zenobi, A.. “CHARACTERIZATION OF MOLECULAR MECHANISMS DRIVING EPIGENETIC CONVERSION AND PHENOTYPE SWITCH OF FIBROBLASTS INTO INSULIN SECRETING CELLS.” 2018. Web. 22 Jan 2021.

Vancouver:

Zenobi A. CHARACTERIZATION OF MOLECULAR MECHANISMS DRIVING EPIGENETIC CONVERSION AND PHENOTYPE SWITCH OF FIBROBLASTS INTO INSULIN SECRETING CELLS. [Internet] [Thesis]. Università degli Studi di Milano; 2018. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/2434/548193.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Zenobi A. CHARACTERIZATION OF MOLECULAR MECHANISMS DRIVING EPIGENETIC CONVERSION AND PHENOTYPE SWITCH OF FIBROBLASTS INTO INSULIN SECRETING CELLS. [Thesis]. Università degli Studi di Milano; 2018. Available from: http://hdl.handle.net/2434/548193

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

5. C. Dieci. Improving in vitro embryo production: the use of oocyte chromatin configuration and cumulus cell gene expression to set up customized pre-IVM protocols.

Degree: 2018, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/574781

► L'efficienza delle tecnologie di produzione di embrioni in vitro (IVP) è ancora limitata sia in ambito zootecnico che in clinica umana. L'IVP consiste in 4… (more)

▼ L'efficienza delle tecnologie di produzione di embrioni in vitro (IVP) è ancora limitata sia in ambito zootecnico che in clinica umana. L'IVP consiste in 4 fasi principali in cui si verificano in laboratorio gli eventi chiave dello sviluppo di un nuovo organismo: 1) il recupero di ovociti immaturi (arrestati in profase I della divisione meiotica) da donatrici vive o isolati da ovaie raccolte da animali macellati; 2) la maturazione in vitro (IVM), cioè la coltura degli ovociti fino allo stadio di metafase II della meiosi; 3) la fecondazione in vitro (IVF) e 4) la coltura in vitro dell'embrione (IVC), durante la quale, gli embrioni di nuova formazione, raggiungono lo stadio di blastocisti. Dopo la fecondazione in vitro, gli embrioni possono essere trasferiti in riceventi attraverso il trasferimento embrionario o essere congelati per un uso successivo. Nonostante i potenziali progressi offerti dai sistemi IVP, la percentuale di successo nella specie bovina è rimasta pressoché stabile negli ultimi 30 anni ed è limitata ad un terzo degli ovociti isolati dall'ovaio che raggiungono lo stadio di blastocisti. Questa condizione è purtroppo comune ad altre specie. La qualità degli ovociti è cruciale per l'efficienza dei protocolli di IVP e la maturazione dell'ovocita rappresenta il primo processo limitante di tutto il sistema. Al momento della rimozione dai follicoli ovarici antrali, gli ovociti si trovano arrestati alla profase della prima divisione meiotica, la cosiddetta fase della vescicola germinale (GV), in cui la cromatina è racchiusa all'interno dell'involucro nucleare. Gli ovociti allo stadio GV abitualmente utilizzati nelle pratiche di IVP sono raccolti da follicoli antrali di medie dimensioni e, in quanto tali, devono ancora completare i passaggi finali di crescita e differenziamento necessari ad acquisire la cosiddetta "competenza di sviluppo", ossia il corredo di organuli e molecole necessario a sostenere la fecondazione e lo sviluppo dell'embrione. Inoltre, poiché lo sviluppo dei follicoli dei mammiferi avviene a ondate successive, uno dei fattori limitanti è rappresentato dall'eterogeneità intrinseca degli ovociti sottoposti a IVM e dalla mancanza di approcci in vitro dedicati per finalizzare il loro processo di differenziamento. Gli studi condotti durante il programma di dottorato mirano a definire parametri morfologici e funzionali non invasivi che possano essere utilizzati come marker del differenziamento degli ovociti e che possano essere considerati per migliorare l'efficienza delle tecniche attuali. Gli studi sono stati condotti sui modelli animali suino e bovino. In entrambe le specie, i cambiamenti progressivi nella configurazione su larga scala della cromatina, che caratterizzano il processo di differenziamento degli ovociti, sono stati utilizzati come marker di acquisizione della competenza allo sviluppo embrionale. In particolare, l'ipotesi di lavoro principale è stata quella di utilizzare la configurazione della cromatina come indicatore dell'eterogeneità della popolazione di ovociti sottoposti a… Advisors/Committee Members: tutor: A.M. Luciano, co-tutor: V. Lodde, coordinatore: F. Gandolfi, GANDOLFI, FULVIO.

Subjects/Keywords: Settore VET/01 - Anatomia degli Animali Domestici

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Dieci, C. (2018). Improving in vitro embryo production: the use of oocyte chromatin configuration and cumulus cell gene expression to set up customized pre-IVM protocols. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/574781

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Dieci, C.. “Improving in vitro embryo production: the use of oocyte chromatin configuration and cumulus cell gene expression to set up customized pre-IVM protocols.” 2018. Thesis, Università degli Studi di Milano. Accessed January 22, 2021. http://hdl.handle.net/2434/574781.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Dieci, C.. “Improving in vitro embryo production: the use of oocyte chromatin configuration and cumulus cell gene expression to set up customized pre-IVM protocols.” 2018. Web. 22 Jan 2021.

Vancouver:

Dieci C. Improving in vitro embryo production: the use of oocyte chromatin configuration and cumulus cell gene expression to set up customized pre-IVM protocols. [Internet] [Thesis]. Università degli Studi di Milano; 2018. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/2434/574781.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Dieci C. Improving in vitro embryo production: the use of oocyte chromatin configuration and cumulus cell gene expression to set up customized pre-IVM protocols. [Thesis]. Università degli Studi di Milano; 2018. Available from: http://hdl.handle.net/2434/574781

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

6. M.L. MATELA DA SILVA AIDOS. ADVANCES IN THE EARLY LARVAL STAGES OF SIBERIAN STURGEON: MUSCLE DEVELOPMENT AND STRUCTURE UNDER DIFFERENT REARING CONDITIONS.

Degree: 2019, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/623828

► Fish larval stages are particularly sensitive to environmental conditions, as these can severely affect survival and potential growth. In fish species destined for production and… (more)

▼ Fish larval stages are particularly sensitive to environmental conditions, as these can severely affect survival and potential growth. In fish species destined for production and commercialisation, a suitable early environment is of great importance, for what concerns growth efficiency and quality at harvest. Temperature and stocking density are among the most important factors affecting the early stages of development, regarding somatic and muscle growth of fish. Moreover, environmental enrichment is considered to improve biological functioning of captive animals, including fish, by improving their psychological and behavioral needs. Siberian sturgeon (Acipenser baerii) is a species at risk of extinction and, therefore, production of this species in aquaculture is of vital importance, not only to provide the market with caviar and meat, but also for repopulation purposes. Taking into account that little is known regarding the impact of environmental conditions in early life stages of the Siberian sturgeon, the aim of this Thesis is to provide new knowledge on the influence on muscle growth and development during the endogenous feeding phase. This was performed by addressing morphological, physiological and molecular aspects of muscle growth structure and development. This Thesis may contribute to identify and select the most suitable environmental conditions to improve Siberian sturgeon larval rearing in aquaculture production. Three trials have been performed, one in each year of my PhD, where 1) rearing temperature, 2) stocking density and 3) environmental enrichment were examined in important timepoints of Siberian sturgeon larvae development: hatching, schooling and complete yolk-sac absorption stage. In Chapter 1, is presented a general overview of Siberian sturgeon biology and development, a brief description of the muscle morphology and of the methods used to study muscle growth. Following, the types of sturgeon muscle growth are described, as well as the mechanisms involved in muscle growth regulation. Finally, the environmental effects on growth are presented along with a short overview of the plasticity of the myogenic phenotype. In Chapters 2, 3 and 4, the effects of three different rearing temperatures (16, 19 or 21 °C) applied from hatching until the yolk-sac full absorption were assessed. Siberian sturgeon fertilized eggs were all incubated at 16°C and newly-hatched larvae were then subjected to three different temperatures throughout the endogenous feeding phase. All the three experimental rearing temperatures were congruent with a correct development of farmed A. baerii (Chapter 2, 3 and 4), but a rearing temperature of 22°C lead to a higher developmental rate, which could be advantageous in commercial hatcheries, as it shortens the endogenous feeding phase and allows to feed the larvae sooner with exogenous feed. In Chapter 2, histometrical, histochemical and immunohistochemical analyses were performed in order to characterize muscle growth (total muscle area, TMA; slow muscle area, SMA; fast muscle… Advisors/Committee Members: tutor: A. Di Giancamillo, coordinator: F. Gandolfi, DI GIANCAMILLO, ALESSIA, GANDOLFI, FULVIO.

Subjects/Keywords: Settore VET/01 - Anatomia degli Animali Domestici

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

AIDOS, M. M. D. S. (2019). ADVANCES IN THE EARLY LARVAL STAGES OF SIBERIAN STURGEON: MUSCLE DEVELOPMENT AND STRUCTURE UNDER DIFFERENT REARING CONDITIONS. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/623828

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

AIDOS, M.L. MATELA DA SILVA. “ADVANCES IN THE EARLY LARVAL STAGES OF SIBERIAN STURGEON: MUSCLE DEVELOPMENT AND STRUCTURE UNDER DIFFERENT REARING CONDITIONS.” 2019. Thesis, Università degli Studi di Milano. Accessed January 22, 2021. http://hdl.handle.net/2434/623828.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

AIDOS, M.L. MATELA DA SILVA. “ADVANCES IN THE EARLY LARVAL STAGES OF SIBERIAN STURGEON: MUSCLE DEVELOPMENT AND STRUCTURE UNDER DIFFERENT REARING CONDITIONS.” 2019. Web. 22 Jan 2021.

Vancouver:

AIDOS MMDS. ADVANCES IN THE EARLY LARVAL STAGES OF SIBERIAN STURGEON: MUSCLE DEVELOPMENT AND STRUCTURE UNDER DIFFERENT REARING CONDITIONS. [Internet] [Thesis]. Università degli Studi di Milano; 2019. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/2434/623828.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

AIDOS MMDS. ADVANCES IN THE EARLY LARVAL STAGES OF SIBERIAN STURGEON: MUSCLE DEVELOPMENT AND STRUCTURE UNDER DIFFERENT REARING CONDITIONS. [Thesis]. Università degli Studi di Milano; 2019. Available from: http://hdl.handle.net/2434/623828

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

7. E.F.M. Manzoni. MOLECULAR AND CELLULAR MECHANISMS REGULATING EPIGENETIC CELL CONVERSION.

Degree: 2019, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/620217

► Phenotype definition is controlled by epigenetic regulations that allow cells to acquire their differentiated state. The process is reversible and cells can be driven back… (more)

▼ Phenotype definition is controlled by epigenetic regulations that allow cells to acquire their differentiated state. The process is reversible and cells can be driven back to a higher plasticity state with several different approaches, which include the interaction with the epigenetic set up through the use of epigenetic erasers, readers or writers. Among the many epigenetic modifiers presently available, in our previous experiments, we selected 5-azacytidine (5-aza-CR), which is a well-known DNA methyltransferase inhibitor, and has been previously shown to increase cell plasticity and facilitate phenotype changes in different cell types. Beside the epigenetic mechanisms driving cell conversion processes, growing evidences highlight the importance of mechanical forces that directly influence cell plasticity and differentiation. Aims of my PhD were: a) characterization of the molecular and cellular mechanisms regulating cell phenotype b) analysis of the possible relation between mechano-sensing and epigenetic control of cell plasticity and differentiation. The experiments carried out confirmed the global demethylating effect of 5-aza-CR, with a transient upregulation of pluripotency markers. At the same time increased transcription of TET2 and several histones was detected. This was accompanied by changes in enzymes controlling histone acetylation and cell morphological rearrangement. Interestingly, the study of DNA methylation profile and its regulatory genes, revealed that the use of a 3D micro-bioreactor promotes and stabilizes the maintenance of the acquired plasticity for a long period of culture. In particular, the use of an adequate soft substrate increased pancreatic conversion efficiency and induced the acquisition of a mono-hormonal phenotype, which is distinctive of terminally matured cell. Altogether these findings indicate that 5-aza-CR induced somatic cell transition to a higher plasticity state may be the result of multiple regulatory mechanisms that accompany the demethylating effect exerted by the modifier. The results described in my thesis also revealed that mechano-transduction-related responses modulate and maintain 5-aza- CR induced cell plasticity and significantly improve cell differentiation toward the pancreatic lineage. Advisors/Committee Members: tutor: F. Gandolfi, supervisore: T. Brevini, coordinatore: F. Gandolfi, GANDOLFI, FULVIO, BREVINI, TIZIANA.

Subjects/Keywords: Settore VET/01 - Anatomia degli Animali Domestici

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APA (6^th Edition):

Manzoni, E. (2019). MOLECULAR AND CELLULAR MECHANISMS REGULATING EPIGENETIC CELL CONVERSION. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/620217

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Manzoni, E.F.M.. “MOLECULAR AND CELLULAR MECHANISMS REGULATING EPIGENETIC CELL CONVERSION.” 2019. Thesis, Università degli Studi di Milano. Accessed January 22, 2021. http://hdl.handle.net/2434/620217.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Manzoni, E.F.M.. “MOLECULAR AND CELLULAR MECHANISMS REGULATING EPIGENETIC CELL CONVERSION.” 2019. Web. 22 Jan 2021.

Vancouver:

Manzoni E. MOLECULAR AND CELLULAR MECHANISMS REGULATING EPIGENETIC CELL CONVERSION. [Internet] [Thesis]. Università degli Studi di Milano; 2019. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/2434/620217.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Manzoni E. MOLECULAR AND CELLULAR MECHANISMS REGULATING EPIGENETIC CELL CONVERSION. [Thesis]. Università degli Studi di Milano; 2019. Available from: http://hdl.handle.net/2434/620217

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

8. M. Aralla. MORPHOLOGICAL FEATURES OF THE REPRODUCTIVE ORGANS IN DOMESTIC SPECIES IN RELATION TO WATER MOVEMENT CONTROL, HYPONUTRITION AND ENVIRONMENTAL INFLUENCES IN FETAL AND PREPUBERTAL LIFE, AND METABOLISM REGULATOR FACTORS.

Degree: 2011, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/153542

► AQPs are a class of small, hydrophobic, integral membrane proteins that are able to make the cell membrane 10 to 100 fold more permeable than… (more)

▼ AQPs are a class of small, hydrophobic, integral membrane proteins that are able to make the cell membrane 10 to 100 fold more permeable than the membranes lacking such channels. Based on studies with AQP1, it appears that AQPs exist in the plasma membrane as homotetramers. Each AQP monomer contains 2 hemi-pores, which fold together to form a water channel. Different aqps have different patterns of glycosylation. In the case of AQP1 the peptide backbone is roughly 28 kDa and the glycosylated forms range from 40 to 60 kDa in mass. Water transport activity of AQP1 is inhibited by mercurial ion, and the site of mercurial inhibition is the cysteine in the E loop. I have personally verified that using the bovine ovary granulosa cell swelling and shrinkage as a model for mercurial inhibition. The shortest pathway for water to reach the follicle antral cavity is the transcellular pathway directly through the cytoplasm of granulosa cells. In this first experiment we have considered the functional presence of Hg2+-sensitive Aqps in isolated granulosa cells assessing changes in cell volume in presence or absence of HgCl2. Each of the AQPs has an essentially unique pattern of expression among tissues and during development. In humans 13 AQPs have been identified to date although this number pales in comparison to the greater than 100 related proteins found in plants and bacteria. The landmark discovery of AQPs starts the era of molecular water channels, which has permeated virtually every branch of biology and medicine. A number of recent studies have revealed the importance of mammalian AQPs in both physiology and pathophysiology and have suggested that pharmacological modulation of aquaporin expression and activity may provide new tools for the treatment of variety of human disorders, such as brain edema, glaucoma, tumour growth, fertility and obesity in which water and small solute transport may be involved. Since multiple isoforms of AQPs are differentially expressed in cells and tissues, their localization to specific cells and membrane domains is critical to understanding the role of each AQP in the transfer of water and small solutes. Balancement of water concentration within the uterine environment is important in every reproductive moment of a female mammal life. Rapid transcellular water movements are facilitated by AQPs that increase plasma membrane permeability. The results from my research showed that AQP-mediated water movement between the intraluminal, interstitial, and capillary compartments is crucial in the uterine imbibition mechanism in the bitch, including periodic stromal oedema in preparation for embryo implantation, with evidence that the expression of different AQPs can be regulated by steroid sex hormones. Also, the importance of the AQP-mediated fluid regulation in uterine environment in the peri-implantation period is known in the woman and laboratory mammals, to assure the successful implantation of blastocyst and its survival. The establishment of pregnancy and its maintenance is… Advisors/Committee Members: tutor: Silvana Arrighi, coordinatore: Valentino Bontempo, direttore scuola dottorato: Vittorio Dell'Orto, ARRIGHI, SILVANA, BONTEMPO, VALENTINO.

Subjects/Keywords: aquaporins; fertility; metabolism; nutrition; Settore VET/01 - Anatomia degli Animali Domestici

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Aralla, M. (2011). MORPHOLOGICAL FEATURES OF THE REPRODUCTIVE ORGANS IN DOMESTIC SPECIES IN RELATION TO WATER MOVEMENT CONTROL, HYPONUTRITION AND ENVIRONMENTAL INFLUENCES IN FETAL AND PREPUBERTAL LIFE, AND METABOLISM REGULATOR FACTORS. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/153542

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Aralla, M.. “MORPHOLOGICAL FEATURES OF THE REPRODUCTIVE ORGANS IN DOMESTIC SPECIES IN RELATION TO WATER MOVEMENT CONTROL, HYPONUTRITION AND ENVIRONMENTAL INFLUENCES IN FETAL AND PREPUBERTAL LIFE, AND METABOLISM REGULATOR FACTORS.” 2011. Thesis, Università degli Studi di Milano. Accessed January 22, 2021. http://hdl.handle.net/2434/153542.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Aralla, M.. “MORPHOLOGICAL FEATURES OF THE REPRODUCTIVE ORGANS IN DOMESTIC SPECIES IN RELATION TO WATER MOVEMENT CONTROL, HYPONUTRITION AND ENVIRONMENTAL INFLUENCES IN FETAL AND PREPUBERTAL LIFE, AND METABOLISM REGULATOR FACTORS.” 2011. Web. 22 Jan 2021.

Vancouver:

Aralla M. MORPHOLOGICAL FEATURES OF THE REPRODUCTIVE ORGANS IN DOMESTIC SPECIES IN RELATION TO WATER MOVEMENT CONTROL, HYPONUTRITION AND ENVIRONMENTAL INFLUENCES IN FETAL AND PREPUBERTAL LIFE, AND METABOLISM REGULATOR FACTORS. [Internet] [Thesis]. Università degli Studi di Milano; 2011. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/2434/153542.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Aralla M. MORPHOLOGICAL FEATURES OF THE REPRODUCTIVE ORGANS IN DOMESTIC SPECIES IN RELATION TO WATER MOVEMENT CONTROL, HYPONUTRITION AND ENVIRONMENTAL INFLUENCES IN FETAL AND PREPUBERTAL LIFE, AND METABOLISM REGULATOR FACTORS. [Thesis]. Università degli Studi di Milano; 2011. Available from: http://hdl.handle.net/2434/153542

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Università degli Studi di Padova

9. Dorotea, Tiziano. Citoarchitettura e caratteristiche neurochimiche dell'archicorteccia del tursiope (Tursiops truncatus).

Degree: 2011, Università degli Studi di Padova

URL: http://tesi.cab.unipd.it/32894/1/tesi_vet_dorotea.pdf

SOno state analizzate la Citoarchitettura e la morfologia cellulare della formazione ippocampale di tursiope (tursiops truncatus) con colorazione di NISS1. Tramite metodo immunoenzimatico Ã¨ stata caratterizzata la distribuzione delle proteine leganti il calcio (Parvalbumina Calbindina e Calretinina) e la presenza e organizzazione della popolazione Astrogliale.

Subjects/Keywords: VET/01 Anatomia degli animali domestici

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Dorotea, T. (2011). Citoarchitettura e caratteristiche neurochimiche dell'archicorteccia del tursiope (Tursiops truncatus). (Thesis). Università degli Studi di Padova. Retrieved from http://tesi.cab.unipd.it/32894/1/tesi_vet_dorotea.pdf

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Dorotea, Tiziano. “Citoarchitettura e caratteristiche neurochimiche dell'archicorteccia del tursiope (Tursiops truncatus).” 2011. Thesis, Università degli Studi di Padova. Accessed January 22, 2021. http://tesi.cab.unipd.it/32894/1/tesi_vet_dorotea.pdf.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Dorotea, Tiziano. “Citoarchitettura e caratteristiche neurochimiche dell'archicorteccia del tursiope (Tursiops truncatus).” 2011. Web. 22 Jan 2021.

Vancouver:

Dorotea T. Citoarchitettura e caratteristiche neurochimiche dell'archicorteccia del tursiope (Tursiops truncatus). [Internet] [Thesis]. Università degli Studi di Padova; 2011. [cited 2021 Jan 22]. Available from: http://tesi.cab.unipd.it/32894/1/tesi_vet_dorotea.pdf.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Dorotea T. Citoarchitettura e caratteristiche neurochimiche dell'archicorteccia del tursiope (Tursiops truncatus). [Thesis]. Università degli Studi di Padova; 2011. Available from: http://tesi.cab.unipd.it/32894/1/tesi_vet_dorotea.pdf

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Università degli Studi di Padova

10. Tutzer, Lorenzo. INDAGINE ISTOCHIMICA E MORFOMETRICA SULLE FIBRE MUSCOLARI DEL M. PECTORALIS SUPERFICIALIS IN POLLI DI RAZZA PADOVANA PURA E IN INCROCI.

Degree: 2012, Università degli Studi di Padova

URL: http://tesi.cab.unipd.it/40591/1/bozza_tesi_14.03_revisionata.pdf

► After pork meat, the most diffused meat is poultry meat. The production of poultry meat is the most increasing development sector and its importance at… (more)

Subjects/Keywords: VET/01 Anatomia degli animali domestici

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Tutzer, L. (2012). INDAGINE ISTOCHIMICA E MORFOMETRICA SULLE FIBRE MUSCOLARI DEL M. PECTORALIS SUPERFICIALIS IN POLLI DI RAZZA PADOVANA PURA E IN INCROCI. (Thesis). Università degli Studi di Padova. Retrieved from http://tesi.cab.unipd.it/40591/1/bozza_tesi_14.03_revisionata.pdf

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Tutzer, Lorenzo. “INDAGINE ISTOCHIMICA E MORFOMETRICA SULLE FIBRE MUSCOLARI DEL M. PECTORALIS SUPERFICIALIS IN POLLI DI RAZZA PADOVANA PURA E IN INCROCI.” 2012. Thesis, Università degli Studi di Padova. Accessed January 22, 2021. http://tesi.cab.unipd.it/40591/1/bozza_tesi_14.03_revisionata.pdf.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Tutzer, Lorenzo. “INDAGINE ISTOCHIMICA E MORFOMETRICA SULLE FIBRE MUSCOLARI DEL M. PECTORALIS SUPERFICIALIS IN POLLI DI RAZZA PADOVANA PURA E IN INCROCI.” 2012. Web. 22 Jan 2021.

Vancouver:

Tutzer L. INDAGINE ISTOCHIMICA E MORFOMETRICA SULLE FIBRE MUSCOLARI DEL M. PECTORALIS SUPERFICIALIS IN POLLI DI RAZZA PADOVANA PURA E IN INCROCI. [Internet] [Thesis]. Università degli Studi di Padova; 2012. [cited 2021 Jan 22]. Available from: http://tesi.cab.unipd.it/40591/1/bozza_tesi_14.03_revisionata.pdf.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Tutzer L. INDAGINE ISTOCHIMICA E MORFOMETRICA SULLE FIBRE MUSCOLARI DEL M. PECTORALIS SUPERFICIALIS IN POLLI DI RAZZA PADOVANA PURA E IN INCROCI. [Thesis]. Università degli Studi di Padova; 2012. Available from: http://tesi.cab.unipd.it/40591/1/bozza_tesi_14.03_revisionata.pdf

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Università di Bologna

11. Canova, Marco. Studio anatomico dei muscoli della spalla e del braccio in Pernis apivorus, Accipiter gentilis ed Accipiter nisus.

Degree: 2015, Università di Bologna

URL: http://amsdottorato.unibo.it/6876/1/canova_marco_tesi.pdf ; Canova, Marco (2015) Studio anatomico dei muscoli della spalla e del braccio in Pernis apivorus, Accipiter gentilis ed Accipiter nisus, [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Scienze veterinarie <http://amsdottorato.unibo.it/view/dottorati/DOT507/>, 27 Ciclo. DOI 10.6092/unibo/amsdottorato/6876.

►

Recentemente, vari ricercatori si sono concentrati sulle proprietà funzionali dei muscoli, sulla meccanica e sulla cinematica legata al volo, ma senza la possibilità di consultare… (more)

▼

Recentemente, vari ricercatori si sono concentrati sulle proprietà funzionali dei muscoli, sulla meccanica e sulla cinematica legata al volo, ma senza la possibilità di consultare un’adeguata letteratura poiché assente o incompleta. Si è quindi ritenuto utile studiare la miologia della regione della spalla e del braccio di specie non in precedenza esaminate e che mostrassero differenti stili di volo, al fine di creare una base di informazioni per futuri studi funzionali. Tutte le specie prese in esame sono membri della famiglia degli Accipitridae e nello specifico si sono selezionati: il Falco pecchiaiolo (Pernis apivorus Linnaeus, 1758), l’Astore comune (Accipiter gentilis Linnaeus, 1758) e lo Sparviero eurasiatico (Accipiter nisus Linnaeus, 1758). Questi animali attuano stili di volo differenti e sono stati pertanto scelti per capire se diverse performance di volo potessero indurre una differenziazione dell’apparato muscolare. La comparazione dei dati raccolti durante questo studio con quelli presenti in letteratura in altre specie aviarie ha evidenziato che, mentre alcuni muscoli non sembrano andare incontro a grandi modificazioni nei rapaci, altri invece presentano una grande eterogeneità. In particolare, grazie alle caratteristiche dei diversi muscoli, si è potuto raggruppare questi ultimi in base a possibili funzioni comuni: a) muscoli che concorrono a stabilizzare l’ala rispetto al tronco e che assorbono le forze generate durante il volo; b) muscoli con possibile ruolo nel controllo della rotazione dell’omero; c) muscoli con possibile funzione propriocettiva. Il presente studio ha inoltre evidenziato, nei muscoli esaminati, alcune similitudini e molteplici peculiarità anatomiche non precedentemente segnalate in letteratura. Si ritiene che questo studio macroscopico possa rappresentare un punto di partenza per futuri studi microscopici o elettrofisiologici, a dimostrazione dell’importanza della dissezione quale primo strumento di indagine.

Many researchers have recently focused on the functional properties of muscles, and on the mechanics and kinematics of flight, without being able to consult an anatomical database since such a database was either non-existent or incomplete. In particular, this study examined the anatomy of the wing muscles of three Accipitridae, such as the European honey buzzard (Pernis apivorus Linnaeus, 1758), the northern goshawk (Accipiter gentilis Linnaeus, 1758) and the eurasian sparrowhawk (Accipiter nisus Linnaeus, 1758). These birds of prey were selected due to their different flight style in order to understand if such different flight performance could induce muscles differentiation. The comparison of the results of this study with other researches shows that some muscles exhibit a lesser degree of differentiation as compared to other previously examined birds of prey. However, in other muscles, many peculiarities were found. Based on the common characteristics of these muscles have been grouped into three sets with possible common function: a) muscles stabilizing the…

Subjects/Keywords: VET/01 Anatomia degli animali domestici

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Canova, M. (2015). Studio anatomico dei muscoli della spalla e del braccio in Pernis apivorus, Accipiter gentilis ed Accipiter nisus. (Thesis). Università di Bologna. Retrieved from http://amsdottorato.unibo.it/6876/1/canova_marco_tesi.pdf ; Canova, Marco (2015) Studio anatomico dei muscoli della spalla e del braccio in Pernis apivorus, Accipiter gentilis ed Accipiter nisus, [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Scienze veterinarie <http://amsdottorato.unibo.it/view/dottorati/DOT507/>, 27 Ciclo. DOI 10.6092/unibo/amsdottorato/6876.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Canova, Marco. “Studio anatomico dei muscoli della spalla e del braccio in Pernis apivorus, Accipiter gentilis ed Accipiter nisus.” 2015. Thesis, Università di Bologna. Accessed January 22, 2021. http://amsdottorato.unibo.it/6876/1/canova_marco_tesi.pdf ; Canova, Marco (2015) Studio anatomico dei muscoli della spalla e del braccio in Pernis apivorus, Accipiter gentilis ed Accipiter nisus, [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Scienze veterinarie <http://amsdottorato.unibo.it/view/dottorati/DOT507/>, 27 Ciclo. DOI 10.6092/unibo/amsdottorato/6876..

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Canova, Marco. “Studio anatomico dei muscoli della spalla e del braccio in Pernis apivorus, Accipiter gentilis ed Accipiter nisus.” 2015. Web. 22 Jan 2021.

Vancouver:

Canova M. Studio anatomico dei muscoli della spalla e del braccio in Pernis apivorus, Accipiter gentilis ed Accipiter nisus. [Internet] [Thesis]. Università di Bologna; 2015. [cited 2021 Jan 22]. Available from: http://amsdottorato.unibo.it/6876/1/canova_marco_tesi.pdf ; Canova, Marco (2015) Studio anatomico dei muscoli della spalla e del braccio in Pernis apivorus, Accipiter gentilis ed Accipiter nisus, [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Scienze veterinarie <http://amsdottorato.unibo.it/view/dottorati/DOT507/>, 27 Ciclo. DOI 10.6092/unibo/amsdottorato/6876..

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Canova M. Studio anatomico dei muscoli della spalla e del braccio in Pernis apivorus, Accipiter gentilis ed Accipiter nisus. [Thesis]. Università di Bologna; 2015. Available from: http://amsdottorato.unibo.it/6876/1/canova_marco_tesi.pdf ; Canova, Marco (2015) Studio anatomico dei muscoli della spalla e del braccio in Pernis apivorus, Accipiter gentilis ed Accipiter nisus, [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Scienze veterinarie <http://amsdottorato.unibo.it/view/dottorati/DOT507/>, 27 Ciclo. DOI 10.6092/unibo/amsdottorato/6876.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Università di Bologna

12. Gardini, Anna. Caratteristiche anatomiche e neurochimiche della formazione ippocampale di cane.

Degree: 2015, Università di Bologna

URL: http://amsdottorato.unibo.it/6975/1/gardini_anna_tesi.pdf ; Gardini, Anna (2015) Caratteristiche anatomiche e neurochimiche della formazione ippocampale di cane, [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Scienze veterinarie <http://amsdottorato.unibo.it/view/dottorati/DOT507/>, 27 Ciclo. DOI 10.6092/unibo/amsdottorato/6975.

►

Nei Roditori e nei Primati, studi di immunoistochimica condotti sulla formazione ippocampale hanno dimostrato che le proteine leganti il calcio (parvalbumina, calbindina-D28k e calretinina) sono… (more)

▼

Nei Roditori e nei Primati, studi di immunoistochimica condotti sulla formazione ippocampale hanno dimostrato che le proteine leganti il calcio (parvalbumina, calbindina-D28k e calretinina) sono dei marker che consentono di identificare differenti sottopopolazioni di neuroni. Nel presente studio è stata analizzata la distribuzione di queste proteine nella formazione ippocampale di cane. L’immunoreattività per la parvalbumina è stata localizzata in neuroni multipolari presenti nello strato polimorfo e nei campi CA3-CA1, così come in alcuni neuroni presumibilmente inibitori localizzati nel campo CA1 e nel subicolo. I granuli e le fibre muschiate presentavano una forte immunoreattività per la calbindina-D28k. Tale immunoreattività era evidente anche nei neuroni piramidali del campo CA1 e del subicolo ed in alcuni interneuroni, presumibilmente inibitori, distribuiti nella formazione ippocampale. L’immunoreatività per la calretinina era relativamente bassa in tutta la formazione ippocampale. Le analisi immunoistochimiche hanno evidenziato, nel giro dentato e nel campo CA1, una riduzione età-dipendente dell’immunoreattività per la parvalbumina e la calretinina. Le analisi condotte mediante risonanza magnetica hanno inoltre dimostrato una riduzione volumetrica età-dipendente della formazione ippocampale di cane.

In Rodents and Primates, immunohistochemical studies of the hippocampal formation have demonstrated that the calcium-binding proteins (parvalbumin, calbindin-D28k, and calretinin) are useful markers to identify different subpopulation of neurons. In the present analysis, we analyzed the distribution of these proteins in the canine hippocampal formation. Parvalbumin immunoreactivity was observed in multipolar neurons in the polymorphic cell layer and throughout the CA3-CA1 fields and in a few presumably interneurons neurons in the CA1 field and subiculum. Calbindin-D28k immunoreactivity displayed a strong labeling of the granule cells and mossy fibers. In addition, this immunoreactivity was also observed pyramidal cells of the CA1 and subiculum as well as in presumably inhibitory interneurons distributed throughout the hippocampal formation. Calretinin immunoreactivity was relatively weaker overall. Immunohistochemistry showed an age-related decrease in calretinin and parvalbumin expression in dentate gyrus and CA1 field. Age-associated changes of magnetic resonance imaging of canine hippocampal formation have also been observed.

Subjects/Keywords: VET/01 Anatomia degli animali domestici

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APA (6^th Edition):

Gardini, A. (2015). Caratteristiche anatomiche e neurochimiche della formazione ippocampale di cane. (Thesis). Università di Bologna. Retrieved from http://amsdottorato.unibo.it/6975/1/gardini_anna_tesi.pdf ; Gardini, Anna (2015) Caratteristiche anatomiche e neurochimiche della formazione ippocampale di cane, [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Scienze veterinarie <http://amsdottorato.unibo.it/view/dottorati/DOT507/>, 27 Ciclo. DOI 10.6092/unibo/amsdottorato/6975.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Gardini, Anna. “Caratteristiche anatomiche e neurochimiche della formazione ippocampale di cane.” 2015. Thesis, Università di Bologna. Accessed January 22, 2021. http://amsdottorato.unibo.it/6975/1/gardini_anna_tesi.pdf ; Gardini, Anna (2015) Caratteristiche anatomiche e neurochimiche della formazione ippocampale di cane, [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Scienze veterinarie <http://amsdottorato.unibo.it/view/dottorati/DOT507/>, 27 Ciclo. DOI 10.6092/unibo/amsdottorato/6975..

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Gardini, Anna. “Caratteristiche anatomiche e neurochimiche della formazione ippocampale di cane.” 2015. Web. 22 Jan 2021.

Vancouver:

Gardini A. Caratteristiche anatomiche e neurochimiche della formazione ippocampale di cane. [Internet] [Thesis]. Università di Bologna; 2015. [cited 2021 Jan 22]. Available from: http://amsdottorato.unibo.it/6975/1/gardini_anna_tesi.pdf ; Gardini, Anna (2015) Caratteristiche anatomiche e neurochimiche della formazione ippocampale di cane, [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Scienze veterinarie <http://amsdottorato.unibo.it/view/dottorati/DOT507/>, 27 Ciclo. DOI 10.6092/unibo/amsdottorato/6975..

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Gardini A. Caratteristiche anatomiche e neurochimiche della formazione ippocampale di cane. [Thesis]. Università di Bologna; 2015. Available from: http://amsdottorato.unibo.it/6975/1/gardini_anna_tesi.pdf ; Gardini, Anna (2015) Caratteristiche anatomiche e neurochimiche della formazione ippocampale di cane, [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Scienze veterinarie <http://amsdottorato.unibo.it/view/dottorati/DOT507/>, 27 Ciclo. DOI 10.6092/unibo/amsdottorato/6975.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Università di Bologna

13. Rambaldi, Anna Maria. The Nervous System of Delphinidae: Neurochenical Studies on Different Central and Peripheral Regions.

Degree: 2017, Università di Bologna

URL: http://amsdottorato.unibo.it/8071/1/Rambaldi_Anna%20Maria_tesi.pdf ; Rambaldi, Anna Maria (2017) The Nervous System of Delphinidae: Neurochenical Studies on Different Central and Peripheral Regions, [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Scienze veterinarie <http://amsdottorato.unibo.it/view/dottorati/DOT507/>, 29 Ciclo. DOI 10.6092/unibo/amsdottorato/8071.

► During the evolutionary path, cetaceans experienced a return to waters and hence had to adapt many of their anatomical and physiological features to this new… (more)

Subjects/Keywords: VET/01 Anatomia degli animali domestici

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APA (6^th Edition):

Rambaldi, A. M. (2017). The Nervous System of Delphinidae: Neurochenical Studies on Different Central and Peripheral Regions. (Thesis). Università di Bologna. Retrieved from http://amsdottorato.unibo.it/8071/1/Rambaldi_Anna%20Maria_tesi.pdf ; Rambaldi, Anna Maria (2017) The Nervous System of Delphinidae: Neurochenical Studies on Different Central and Peripheral Regions, [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Scienze veterinarie <http://amsdottorato.unibo.it/view/dottorati/DOT507/>, 29 Ciclo. DOI 10.6092/unibo/amsdottorato/8071.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Rambaldi, Anna Maria. “The Nervous System of Delphinidae: Neurochenical Studies on Different Central and Peripheral Regions.” 2017. Thesis, Università di Bologna. Accessed January 22, 2021. http://amsdottorato.unibo.it/8071/1/Rambaldi_Anna%20Maria_tesi.pdf ; Rambaldi, Anna Maria (2017) The Nervous System of Delphinidae: Neurochenical Studies on Different Central and Peripheral Regions, [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Scienze veterinarie <http://amsdottorato.unibo.it/view/dottorati/DOT507/>, 29 Ciclo. DOI 10.6092/unibo/amsdottorato/8071..

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Rambaldi, Anna Maria. “The Nervous System of Delphinidae: Neurochenical Studies on Different Central and Peripheral Regions.” 2017. Web. 22 Jan 2021.

Vancouver:

Rambaldi AM. The Nervous System of Delphinidae: Neurochenical Studies on Different Central and Peripheral Regions. [Internet] [Thesis]. Università di Bologna; 2017. [cited 2021 Jan 22]. Available from: http://amsdottorato.unibo.it/8071/1/Rambaldi_Anna%20Maria_tesi.pdf ; Rambaldi, Anna Maria (2017) The Nervous System of Delphinidae: Neurochenical Studies on Different Central and Peripheral Regions, [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Scienze veterinarie <http://amsdottorato.unibo.it/view/dottorati/DOT507/>, 29 Ciclo. DOI 10.6092/unibo/amsdottorato/8071..

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Rambaldi AM. The Nervous System of Delphinidae: Neurochenical Studies on Different Central and Peripheral Regions. [Thesis]. Università di Bologna; 2017. Available from: http://amsdottorato.unibo.it/8071/1/Rambaldi_Anna%20Maria_tesi.pdf ; Rambaldi, Anna Maria (2017) The Nervous System of Delphinidae: Neurochenical Studies on Different Central and Peripheral Regions, [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Scienze veterinarie <http://amsdottorato.unibo.it/view/dottorati/DOT507/>, 29 Ciclo. DOI 10.6092/unibo/amsdottorato/8071.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

14. Bianco, Francesca. New Insights into Molecular Mechanisms of Enteric Neuro-Epithelial Pathology: From Damage to Protection.

Degree: 2018, Università di Bologna

URL: http://amsdottorato.unibo.it/8376/1/Francesca_Bianco_tesi.pdf ; Bianco, Francesca (2018) New Insights into Molecular Mechanisms of Enteric Neuro-Epithelial Pathology: From Damage to Protection, [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Scienze veterinarie <http://amsdottorato.unibo.it/view/dottorati/DOT507/>, 30 Ciclo. DOI 10.6092/unibo/amsdottorato/8376.

► Backgrond: Functional gastrointestinal disorders (FGID) are chronic conditions characterized by symptoms for which no overt organic cause can be found. They affect up to 20%… (more)

▼ Backgrond: Functional gastrointestinal disorders (FGID) are chronic conditions characterized by symptoms for which no overt organic cause can be found. They affect up to 20% of western populations with evidence suggesting they are equally common in the Third World (Spiller 2005). Although symptoms are generally mild or moderate, a small subset of cases shows severe manifestations, i.e. nausea, vomiting, bloating, abdominal distension, intractable constipation and chronic pain, with such an intensity hampering normal feeding and compromising considerably patients’ quality of life. In addition, this subset of patients may also have recurrent intestinal sub-occlusive episodes, which occur in the absence of demonstrable mechanical causes, leading to numerous hospitalizations as well as useless and potentially harmful surgical interventions. This condition is referred to as chronic intestinal pseudo-obstruction (CIPO), a rare and intractable chronic digestive disease with symptoms and signs of intestinal obstruction without demonstrable mechanical cause. CIPO can result from derangements affecting the integrity of a variety of regulatory cells/tissues, i.e. smooth muscle cells, the interstitial cells of Cajal (ICC) (pace-makers of gut motility), and neurons (either intrinsic – the enteric nervous system- or extrinsic nerve pathways). Aims and experimental studies: The present thesis was conceived to provide a thorough account of the molecular defects underlying CIPO and highlight new strategies of neuro-epithelial protection to prevent enteric neurodegeneration. The experimental studies are based on genetic analysis of CIPO patients and characterization of RAD21 in the ENS of human and mouse intestine. Other studies are focus on 5-hydroxytryptamine-4 (5-HT4) receptor particularly studying whether the activation of colonic epithelial 5-HT4 protects against experimentally induced inflammation of the mouse colon. Conclusion: My thesis focuses on both mechanisms of neurodegeneration and neuro/epithelial protection as a paradigm to better understand both pathophysiology of severe conditions such as CIPO, and related targeted therapeutic options.

Subjects/Keywords: VET/01 Anatomia degli animali domestici

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APA (6^th Edition):

Bianco, F. (2018). New Insights into Molecular Mechanisms of Enteric Neuro-Epithelial Pathology: From Damage to Protection. (Thesis). Università di Bologna. Retrieved from http://amsdottorato.unibo.it/8376/1/Francesca_Bianco_tesi.pdf ; Bianco, Francesca (2018) New Insights into Molecular Mechanisms of Enteric Neuro-Epithelial Pathology: From Damage to Protection, [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Scienze veterinarie <http://amsdottorato.unibo.it/view/dottorati/DOT507/>, 30 Ciclo. DOI 10.6092/unibo/amsdottorato/8376.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Bianco, Francesca. “New Insights into Molecular Mechanisms of Enteric Neuro-Epithelial Pathology: From Damage to Protection.” 2018. Thesis, Università di Bologna. Accessed January 22, 2021. http://amsdottorato.unibo.it/8376/1/Francesca_Bianco_tesi.pdf ; Bianco, Francesca (2018) New Insights into Molecular Mechanisms of Enteric Neuro-Epithelial Pathology: From Damage to Protection, [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Scienze veterinarie <http://amsdottorato.unibo.it/view/dottorati/DOT507/>, 30 Ciclo. DOI 10.6092/unibo/amsdottorato/8376..

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Bianco, Francesca. “New Insights into Molecular Mechanisms of Enteric Neuro-Epithelial Pathology: From Damage to Protection.” 2018. Web. 22 Jan 2021.

Vancouver:

Bianco F. New Insights into Molecular Mechanisms of Enteric Neuro-Epithelial Pathology: From Damage to Protection. [Internet] [Thesis]. Università di Bologna; 2018. [cited 2021 Jan 22]. Available from: http://amsdottorato.unibo.it/8376/1/Francesca_Bianco_tesi.pdf ; Bianco, Francesca (2018) New Insights into Molecular Mechanisms of Enteric Neuro-Epithelial Pathology: From Damage to Protection, [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Scienze veterinarie <http://amsdottorato.unibo.it/view/dottorati/DOT507/>, 30 Ciclo. DOI 10.6092/unibo/amsdottorato/8376..

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Bianco F. New Insights into Molecular Mechanisms of Enteric Neuro-Epithelial Pathology: From Damage to Protection. [Thesis]. Università di Bologna; 2018. Available from: http://amsdottorato.unibo.it/8376/1/Francesca_Bianco_tesi.pdf ; Bianco, Francesca (2018) New Insights into Molecular Mechanisms of Enteric Neuro-Epithelial Pathology: From Damage to Protection, [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Scienze veterinarie <http://amsdottorato.unibo.it/view/dottorati/DOT507/>, 30 Ciclo. DOI 10.6092/unibo/amsdottorato/8376.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Università di Bologna

15. Tagliavia, Claudio. Studio morfologico e morfometrico della stratigrafia di parete dell'apparato gastroenterico di coniglio: aspetti comparativi tra l'esame anatomico microscopico e l'indagine ultrasonografica "in-vivo" ed "ex-vivo".

Degree: 2019, Università di Bologna

URL: http://amsdottorato.unibo.it/8747/3/tagliavia_claudio_tesi.pdf ; Tagliavia, Claudio (2019) Studio morfologico e morfometrico della stratigrafia di parete dell'apparato gastroenterico di coniglio: aspetti comparativi tra l'esame anatomico microscopico e l'indagine ultrasonografica "in-vivo" ed "ex-vivo", [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Scienze veterinarie <http://amsdottorato.unibo.it/view/dottorati/DOT507/>, 31 Ciclo.

►

Negli ultimi anni si è assistito ad una maggiore diffusione dei cosiddetti nuovi animali da compagnia, tra i quali si annovera il coniglio. Ne è… (more)

▼

Negli ultimi anni si è assistito ad una maggiore diffusione dei cosiddetti nuovi animali da compagnia, tra i quali si annovera il coniglio. Ne è conseguito un aumento della richiesta di visite veterinarie e la necessità di implementare le conoscenze specialistiche. Nasce così l’esigenza di conoscere le caratteristiche anatomiche, fisiologiche e patologiche specifiche della specie, ben diverse da quella degli animali domestici comunemente trattati. È in questo contesto che si inquadra la ricerca oggetto di questa tesi, in cui si è voluto indagare - da un punto di vista morfologico e morfometrico - l’apparato gastrointestinale di coniglio, utilizzando due tecniche solo apparentemente lontane tra loro: l’anatomia microscopica e l’indagine ecografica “in-vivo” ed “ex-vivo”. L’indagine microscopica ha consentito di valutare con precisione le normali caratteristiche della parete di ciascun tratto preso in esame, misurando gli spessori delle quattro tonache (sierosa, muscolare, sottomucosa e mucosa) e ricavandone i valori assoluti e relativi percentuali. Questi dati sono quindi serviti come valori di riferimento per valutare la correttezza delle medesime osservazioni e misurazioni ottenute con l’esame ecografico e facilitarne l’interpretazione. Dallo studio è emerso che l’indagine ecografica si è dimostrata attendibile per la misurazione dello spessore totale e parziale della sottomucosa e della muscolare, mentre per le restanti tonache, sierosa e mucosa, il valore non è risultato coerente con il dato anatomico. Questo, presumibilmente, è legato alla limitata capacità di risoluzione della sonda ed ai fenomeni di attenuazione, diffusione ed alla risoluzione assiale. Nel complesso ci si auspica che sia l’indagine anatomica che quella ultrasonografica ad alta risoluzione, svolte nel presente studio sull’apparato gastroenterico di soggetti sani, rappresentino le basi per una migliore comprensione dei reperti anatomopatologici e dei quadri ecografici in presenza di lesioni gastrointestinali, con conseguenti importanti implicazioni terapeutiche e prognostiche.

In recent years there has been a wider spread of so-called new pets, among them is the rabbit. This led to an increase in the request for veterinary examination and the need to implement specialist knowledge. This leads to the requirement of knowing the specific anatomical, physiological and pathological characteristics of the species, significantly different from the commonly treated domestic animals. It is in this context that the research object of this thesis is framed, in which the aim was to investigate - from a morphological and morphometric point of view - the rabbit gastrointestinal system, using two techniques which are only apparently distant from each other: the microscopic anatomy and the “in-vivo” and “ex-vivo” ultrasound. The microscopic investigation allowed for the accurate evaluation of the normal wall characteristics of each section taken into consideration, measuring the thicknesses of the four tunics (serosa, muscularis, submucosa and mucosa)…

Subjects/Keywords: VET/01 Anatomia degli animali domestici

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Tagliavia, C. (2019). Studio morfologico e morfometrico della stratigrafia di parete dell'apparato gastroenterico di coniglio: aspetti comparativi tra l'esame anatomico microscopico e l'indagine ultrasonografica "in-vivo" ed "ex-vivo". (Thesis). Università di Bologna. Retrieved from http://amsdottorato.unibo.it/8747/3/tagliavia_claudio_tesi.pdf ; Tagliavia, Claudio (2019) Studio morfologico e morfometrico della stratigrafia di parete dell'apparato gastroenterico di coniglio: aspetti comparativi tra l'esame anatomico microscopico e l'indagine ultrasonografica "in-vivo" ed "ex-vivo", [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Scienze veterinarie <http://amsdottorato.unibo.it/view/dottorati/DOT507/>, 31 Ciclo.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Tagliavia, Claudio. “Studio morfologico e morfometrico della stratigrafia di parete dell'apparato gastroenterico di coniglio: aspetti comparativi tra l'esame anatomico microscopico e l'indagine ultrasonografica "in-vivo" ed "ex-vivo".” 2019. Thesis, Università di Bologna. Accessed January 22, 2021. http://amsdottorato.unibo.it/8747/3/tagliavia_claudio_tesi.pdf ; Tagliavia, Claudio (2019) Studio morfologico e morfometrico della stratigrafia di parete dell'apparato gastroenterico di coniglio: aspetti comparativi tra l'esame anatomico microscopico e l'indagine ultrasonografica "in-vivo" ed "ex-vivo", [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Scienze veterinarie <http://amsdottorato.unibo.it/view/dottorati/DOT507/>, 31 Ciclo..

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Tagliavia, Claudio. “Studio morfologico e morfometrico della stratigrafia di parete dell'apparato gastroenterico di coniglio: aspetti comparativi tra l'esame anatomico microscopico e l'indagine ultrasonografica "in-vivo" ed "ex-vivo".” 2019. Web. 22 Jan 2021.

Vancouver:

Tagliavia C. Studio morfologico e morfometrico della stratigrafia di parete dell'apparato gastroenterico di coniglio: aspetti comparativi tra l'esame anatomico microscopico e l'indagine ultrasonografica "in-vivo" ed "ex-vivo". [Internet] [Thesis]. Università di Bologna; 2019. [cited 2021 Jan 22]. Available from: http://amsdottorato.unibo.it/8747/3/tagliavia_claudio_tesi.pdf ; Tagliavia, Claudio (2019) Studio morfologico e morfometrico della stratigrafia di parete dell'apparato gastroenterico di coniglio: aspetti comparativi tra l'esame anatomico microscopico e l'indagine ultrasonografica "in-vivo" ed "ex-vivo", [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Scienze veterinarie <http://amsdottorato.unibo.it/view/dottorati/DOT507/>, 31 Ciclo..

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Tagliavia C. Studio morfologico e morfometrico della stratigrafia di parete dell'apparato gastroenterico di coniglio: aspetti comparativi tra l'esame anatomico microscopico e l'indagine ultrasonografica "in-vivo" ed "ex-vivo". [Thesis]. Università di Bologna; 2019. Available from: http://amsdottorato.unibo.it/8747/3/tagliavia_claudio_tesi.pdf ; Tagliavia, Claudio (2019) Studio morfologico e morfometrico della stratigrafia di parete dell'apparato gastroenterico di coniglio: aspetti comparativi tra l'esame anatomico microscopico e l'indagine ultrasonografica "in-vivo" ed "ex-vivo", [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Scienze veterinarie <http://amsdottorato.unibo.it/view/dottorati/DOT507/>, 31 Ciclo.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Università di Bologna

16. Giancola, Fiorella. Interspecies study of the enteric nervous system and related pathologies.

Degree: 2016, Università di Bologna

URL: http://amsdottorato.unibo.it/7579/1/Giancola_Fiorella_Tesi.pdf ; Giancola, Fiorella (2016) Studio interspecie del sistema nervoso enterico e delle patologie ad esso correlate, [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Scienze veterinarie <http://amsdottorato.unibo.it/view/dottorati/DOT507/>, 28 Ciclo. DOI 10.6092/unibo/amsdottorato/7579.

► The enteric nervous system (ENS) modulates a number of digestive functions including well known ones, i.e. motility, secretion, absorption and blood flow, along with other… (more)

▼ The enteric nervous system (ENS) modulates a number of digestive functions including well known ones, i.e. motility, secretion, absorption and blood flow, along with other critically relevant processes, i.e. immune responses of the gastrointestinal (GI) tract, gut microbiota and epithelial barrier . The characterization of the anatomical aspects of the ENS in large mammals and the identification of differences and similarities existing between species may represent a fundamental basis to decipher several digestive GI diseases in humans and animals. In this perspective, the aim of the present thesis is to highlight the ENS anatomical basis and pathological aspects in different mammalian species, such as horses, dogs and humans. Firstly, I designed two anatomical studies in horses:  “Excitatory and inhibitory enteric innervation of horse lower esophageal sphincter”.  “Localization of 5-hydroxytryptamine 4 receptor (5-HT4R) in the equine enteric nervous system”. Then I focused on the enteric dysfunctions, including:  A primary enteric aganglionosis in horses: “Extrinsic innervation of the ileum and pelvic flexure of foals with ileocolonic aganglionosis”.  A diabetic enteric neuropathy in dogs: “Quantification of nitrergic neurons in the myenteric plexus of gastric antrum and ileum of healthy and diabetic dogs”.  An enteric neuropathy in human neurological patients: “Functional and neurochemical abnormalities in patients with Parkinson's disease and chronic constipation”. The physiology of the GI tract is characterized by a high complexity and it is mainly dependent on the control of the intrinsic nervous system. ENS is critical to preserve body homeostasis as reflect by its derangement occurring in pathological conditions that can be lethal or seriously disabling to humans and animals. The knowledge of the anatomy and the pathology of the ENS represents a new important and fascinating topic, which deserves more attention in the veterinary medicine field.

Subjects/Keywords: VET/01 Anatomia degli animali domestici

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Giancola, F. (2016). Interspecies study of the enteric nervous system and related pathologies. (Thesis). Università di Bologna. Retrieved from http://amsdottorato.unibo.it/7579/1/Giancola_Fiorella_Tesi.pdf ; Giancola, Fiorella (2016) Studio interspecie del sistema nervoso enterico e delle patologie ad esso correlate, [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Scienze veterinarie <http://amsdottorato.unibo.it/view/dottorati/DOT507/>, 28 Ciclo. DOI 10.6092/unibo/amsdottorato/7579.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Giancola, Fiorella. “Interspecies study of the enteric nervous system and related pathologies.” 2016. Thesis, Università di Bologna. Accessed January 22, 2021. http://amsdottorato.unibo.it/7579/1/Giancola_Fiorella_Tesi.pdf ; Giancola, Fiorella (2016) Studio interspecie del sistema nervoso enterico e delle patologie ad esso correlate, [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Scienze veterinarie <http://amsdottorato.unibo.it/view/dottorati/DOT507/>, 28 Ciclo. DOI 10.6092/unibo/amsdottorato/7579..

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Giancola, Fiorella. “Interspecies study of the enteric nervous system and related pathologies.” 2016. Web. 22 Jan 2021.

Vancouver:

Giancola F. Interspecies study of the enteric nervous system and related pathologies. [Internet] [Thesis]. Università di Bologna; 2016. [cited 2021 Jan 22]. Available from: http://amsdottorato.unibo.it/7579/1/Giancola_Fiorella_Tesi.pdf ; Giancola, Fiorella (2016) Studio interspecie del sistema nervoso enterico e delle patologie ad esso correlate, [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Scienze veterinarie <http://amsdottorato.unibo.it/view/dottorati/DOT507/>, 28 Ciclo. DOI 10.6092/unibo/amsdottorato/7579..

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Giancola F. Interspecies study of the enteric nervous system and related pathologies. [Thesis]. Università di Bologna; 2016. Available from: http://amsdottorato.unibo.it/7579/1/Giancola_Fiorella_Tesi.pdf ; Giancola, Fiorella (2016) Studio interspecie del sistema nervoso enterico e delle patologie ad esso correlate, [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Scienze veterinarie <http://amsdottorato.unibo.it/view/dottorati/DOT507/>, 28 Ciclo. DOI 10.6092/unibo/amsdottorato/7579.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Università di Bologna

17. Asti, Martina. Sistema nervoso enterico di cane: studio quali-quantitativo e modificazioni neurochimiche in corso di infiammazione gastroenterica e diabete mellito.

Degree: 2016, Università di Bologna

URL: http://amsdottorato.unibo.it/7677/1/Tesi_Asti__PDF.pdf ; Asti, Martina (2016) Sistema nervoso enterico di cane: studio quali-quantitativo e modificazioni neurochimiche in corso di infiammazione gastroenterica e diabete mellito, [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Scienze veterinarie <http://amsdottorato.unibo.it/view/dottorati/DOT507/>, 27 Ciclo. DOI 10.6092/unibo/amsdottorato/7677.

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1) Valutazione caratteristiche neurochimiche e quantificazione dei neuroni del SNE dell’ileo di cani ctrl e cani affetti da infiammazione intestinale (spontanea). Prelevati campioni di ileo… (more)

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1) Valutazione caratteristiche neurochimiche e quantificazione dei neuroni del SNE dell’ileo di cani ctrl e cani affetti da infiammazione intestinale (spontanea). Prelevati campioni di ileo da 5 cani ctrl e 8 cani patol ed ottenute criosezioni sottoposte ad immunoistochimica. - nNOS-IR MP ctrl 33±15% (178/639 cell, n=5) vs patol 24±5% (528/2031 cell, n=7) (P=0,156). nNOS-IR SMP ctrl 8±5% (40/527 cell, n=5) vs patol 7±2% (69/1007 cell, n=4) (P= 0,735). - VIP-IR MP ctrl 6±4% (69/993 cell) vs patol 16±9% (281/1958 cell, n=8) (P=0,027*). VIP-IR SMP ctrl 29±8% (300/993 cell) vs patol 30±13% (522/1630 cell, n=7) (P=0,891,) - SP-IR MP ctrl 15±8% (209/1332 cell, n=5) vs patol 17±9% (437/2053 cell, n=8) (P=0,741). SP-IR SMP ctrl 26±7% (464/1598 cell, n=5) vs patol 24±13% (592/2024 cell, n=6) (P=0,752). - CGRP-IR MP ctrl 8±9% (41/543 cell, n=5) vs patol 16±11% (259/1444 cell, n=8) (P=0,152). CGRP-IR SMP ctrl 7±8% (32/754 cell, n=5) vs patol 14±12% (194/1138 cell, n=7) (P=0,230) - Calb-IR MP ctrl 14±9% (76/580 cell, n=5) vs patol 16±7% (324/2055 cell, n=8)(P=0,596). CGRP-IR SMP ctrl di 22±7% (218/975 cell, n=5) vs patol 23±7% (261/1207 cell, n=7) (P=0,767). Questo rappresenta il primo studio esistente sulle caratteristiche neurochimiche delle sottopopolazioni di neuroni in cani ctrl e cani affetti da patol gastroenterica. 2) Valutazione degli effetti del diabete mellito di tipo I sul SNE dell’antro pilorico e dell’ileo di cane. Prelevati campioni di antro e ileo di 8 cani ctrl e 5 patol ed ottenute criosezioni sottoposte ad immunoistochimica. nNOS-IR MP ctrl antro 30±6%, (902/3129 cellule), ileo 29±5% (795/2800 cellule). nNOS-IR MP DM antro 25±2% (727/2926 cellule), ileo (19±5%; 308/1508 cellule).(P antro=0.112), (P ileo=0.006). Questi risultati indicano che il DM determini un’alterazione dell’innervazione nitrergica maggiore rispetto nell’ileo rispetto allo stomaco.

1) Study of effects of spontaneous inflammatory bowel disease in SNE of dogs. Specimens of ileum were collected from 5 control and 8 affected dogs, use for immunohistochemistry on the cryosections - nNOS-IR MP ctrl 33±15% (178/639 cell, n=5) vs patol 24±5% (528/2031 cell, n=7) (P=0,156). nNOS-IR SMP ctrl 8±5% (40/527 cell, n=5) vs patol 7±2% (69/1007 cell, n=4) (P= 0,735). - VIP-IR MP ctrl 6±4% (69/993 cell) vs patol 16±9% (281/1958 cell, n=8) (P=0,027*). VIP-IR SMP ctrl 29±8% (300/993 cell) vs patol 30±13% (522/1630 cell, n=7) (P=0,891,) - SP-IR MP ctrl 15±8% (209/1332 cell, n=5) vs patol 17±9% (437/2053 cell, n=8) (P=0,741). SP-IR SMP ctrl 26±7% (464/1598 cell, n=5) vs patol 24±13% (592/2024 cell, n=6) (P=0,752). - CGRP-IR MP ctrl 8±9% (41/543 cell, n=5) vs patol 16±11% (259/1444 cell, n=8) (P=0,152). CGRP-IR SMP ctrl 7±8% (32/754 cell, n=5) vs patol 14±12% (194/1138 cell, n=7) (P=0,230) - Calb-IR MP ctrl 14±9% (76/580 cell, n=5) vs patol 16±7% (324/2055 cell, n=8)(P=0,596). CGRP-IR SMP ctrl di 22±7% (218/975 cell, n=5) vs patol 23±7% (261/1207 cell, n=7) (P=0,767). This is the first study about neurochemical characteristics of neuron subsets in…

Subjects/Keywords: VET/01 Anatomia degli animali domestici

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Asti, M. (2016). Sistema nervoso enterico di cane: studio quali-quantitativo e modificazioni neurochimiche in corso di infiammazione gastroenterica e diabete mellito. (Thesis). Università di Bologna. Retrieved from http://amsdottorato.unibo.it/7677/1/Tesi_Asti__PDF.pdf ; Asti, Martina (2016) Sistema nervoso enterico di cane: studio quali-quantitativo e modificazioni neurochimiche in corso di infiammazione gastroenterica e diabete mellito, [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Scienze veterinarie <http://amsdottorato.unibo.it/view/dottorati/DOT507/>, 27 Ciclo. DOI 10.6092/unibo/amsdottorato/7677.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Asti, Martina. “Sistema nervoso enterico di cane: studio quali-quantitativo e modificazioni neurochimiche in corso di infiammazione gastroenterica e diabete mellito.” 2016. Thesis, Università di Bologna. Accessed January 22, 2021. http://amsdottorato.unibo.it/7677/1/Tesi_Asti__PDF.pdf ; Asti, Martina (2016) Sistema nervoso enterico di cane: studio quali-quantitativo e modificazioni neurochimiche in corso di infiammazione gastroenterica e diabete mellito, [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Scienze veterinarie <http://amsdottorato.unibo.it/view/dottorati/DOT507/>, 27 Ciclo. DOI 10.6092/unibo/amsdottorato/7677..

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Asti, Martina. “Sistema nervoso enterico di cane: studio quali-quantitativo e modificazioni neurochimiche in corso di infiammazione gastroenterica e diabete mellito.” 2016. Web. 22 Jan 2021.

Vancouver:

Asti M. Sistema nervoso enterico di cane: studio quali-quantitativo e modificazioni neurochimiche in corso di infiammazione gastroenterica e diabete mellito. [Internet] [Thesis]. Università di Bologna; 2016. [cited 2021 Jan 22]. Available from: http://amsdottorato.unibo.it/7677/1/Tesi_Asti__PDF.pdf ; Asti, Martina (2016) Sistema nervoso enterico di cane: studio quali-quantitativo e modificazioni neurochimiche in corso di infiammazione gastroenterica e diabete mellito, [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Scienze veterinarie <http://amsdottorato.unibo.it/view/dottorati/DOT507/>, 27 Ciclo. DOI 10.6092/unibo/amsdottorato/7677..

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Asti M. Sistema nervoso enterico di cane: studio quali-quantitativo e modificazioni neurochimiche in corso di infiammazione gastroenterica e diabete mellito. [Thesis]. Università di Bologna; 2016. Available from: http://amsdottorato.unibo.it/7677/1/Tesi_Asti__PDF.pdf ; Asti, Martina (2016) Sistema nervoso enterico di cane: studio quali-quantitativo e modificazioni neurochimiche in corso di infiammazione gastroenterica e diabete mellito, [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Scienze veterinarie <http://amsdottorato.unibo.it/view/dottorati/DOT507/>, 27 Ciclo. DOI 10.6092/unibo/amsdottorato/7677.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Università di Bologna

18. Nenzi, Alice. Innervazione articolare della spalla e del ginocchio nel cane e modificazioni in corso di osteoartrite.

Degree: 2011, Università di Bologna

URL: http://amsdottorato.unibo.it/3614/1/nenzi_alice_tesi.pdf ; Nenzi, Alice (2011) Innervazione articolare della spalla e del ginocchio nel cane e modificazioni in corso di osteoartrite, [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Morfofisiologia veterinaria e applicazioni biotecnologiche <http://amsdottorato.unibo.it/view/dottorati/DOT398/>, 23 Ciclo.

► L’osteoartrite (OA) rappresenta un gruppo di patologie a carico delle articolazioni che si stima colpisca più del 20% dei cani con età superiore a un… (more)

▼ L’osteoartrite (OA) rappresenta un gruppo di patologie a carico delle articolazioni che si stima colpisca più del 20% dei cani con età superiore a un anno. Si tratta di una condizione dove entrano in gioco fattori biomeccanici e biochimici, che si esprime clinicamente con zoppia e dolore. Le ricerche effettuate negli ultimi decenni hanno dimostrato il coinvolgimento dell’innervazione nocicettiva e simpatica nell’insorgenza e nel mantenimento del dolore e dell’infiammazione che caratterizzano l’OA. In questa, infatti, svolgono un ruolo cruciale i neuropeptidi, quali la sostanza P (SP) ed il peptide correlato al gene della calcitonina (CGRP), ed i neuromediatori simpatici liberati alla periferia rispettivamente dalle fibre nocicettive e dalle fibre simpatiche postgangliari. Col presente lavoro ci si è posti l’obiettivo di definire il tipo ed il numero di fibre nervose che innervano l’articolazione della spalla e del ginocchio di cane in soggetti esenti da patologia articolare ed affetti da osteoartrite. Campioni di legamenti e capsula articolare della spalla e della parte craniolaterale della capsula articolare del ginocchio sono stati sottoposto a reazione di doppia immunofluorescenza indiretta volta alla identificazione del numero complessivo di fibre nervose presenti e della percentuale di fibre SP, CGRP e simpatiche positive. I risultati testimoniano come in corso di OA nel cane venga ad alterarsi l’equilibrio tra innervazione sensitiva e simpatica. Infatti, lo studio quantitativo ha evidenziato che nei soggetti affetti da OA si assiste ad un incremento della percentuale di fibre SP positive e ad un decremento di quelle simpatiche. Si ritiene che l’integrità di questi due tipi d’innervazione sia necessaria per l’omeostasi tissutale e che un aumento delle fibre nocicettive e una riduzione di quelle simpatiche abbia un effetto proinfiammatorio e iperalgesico. È stata inoltre riscontrata una differente densità di fibre nervose a seconda del tipo di patologia considerata.

Subjects/Keywords: VET/01 Anatomia degli animali domestici

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Nenzi, A. (2011). Innervazione articolare della spalla e del ginocchio nel cane e modificazioni in corso di osteoartrite. (Thesis). Università di Bologna. Retrieved from http://amsdottorato.unibo.it/3614/1/nenzi_alice_tesi.pdf ; Nenzi, Alice (2011) Innervazione articolare della spalla e del ginocchio nel cane e modificazioni in corso di osteoartrite, [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Morfofisiologia veterinaria e applicazioni biotecnologiche <http://amsdottorato.unibo.it/view/dottorati/DOT398/>, 23 Ciclo.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Nenzi, Alice. “Innervazione articolare della spalla e del ginocchio nel cane e modificazioni in corso di osteoartrite.” 2011. Thesis, Università di Bologna. Accessed January 22, 2021. http://amsdottorato.unibo.it/3614/1/nenzi_alice_tesi.pdf ; Nenzi, Alice (2011) Innervazione articolare della spalla e del ginocchio nel cane e modificazioni in corso di osteoartrite, [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Morfofisiologia veterinaria e applicazioni biotecnologiche <http://amsdottorato.unibo.it/view/dottorati/DOT398/>, 23 Ciclo..

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Nenzi, Alice. “Innervazione articolare della spalla e del ginocchio nel cane e modificazioni in corso di osteoartrite.” 2011. Web. 22 Jan 2021.

Vancouver:

Nenzi A. Innervazione articolare della spalla e del ginocchio nel cane e modificazioni in corso di osteoartrite. [Internet] [Thesis]. Università di Bologna; 2011. [cited 2021 Jan 22]. Available from: http://amsdottorato.unibo.it/3614/1/nenzi_alice_tesi.pdf ; Nenzi, Alice (2011) Innervazione articolare della spalla e del ginocchio nel cane e modificazioni in corso di osteoartrite, [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Morfofisiologia veterinaria e applicazioni biotecnologiche <http://amsdottorato.unibo.it/view/dottorati/DOT398/>, 23 Ciclo..

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Nenzi A. Innervazione articolare della spalla e del ginocchio nel cane e modificazioni in corso di osteoartrite. [Thesis]. Università di Bologna; 2011. Available from: http://amsdottorato.unibo.it/3614/1/nenzi_alice_tesi.pdf ; Nenzi, Alice (2011) Innervazione articolare della spalla e del ginocchio nel cane e modificazioni in corso di osteoartrite, [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Morfofisiologia veterinaria e applicazioni biotecnologiche <http://amsdottorato.unibo.it/view/dottorati/DOT398/>, 23 Ciclo.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Università di Bologna

19. Stanzani, Agnese. Aspetti Morfo-Fisiologici dell’Apparato Digerente nei Mammiferi.

Degree: 2020, Università di Bologna

URL: http://amsdottorato.unibo.it/9160/1/stanzani_agnese_tesi.pdf ; Stanzani, Agnese (2020) Aspetti Morfo-Fisiologici dell’Apparato Digerente nei Mammiferi, [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Scienze veterinarie <http://amsdottorato.unibo.it/view/dottorati/DOT507/>, 32 Ciclo. DOI 10.6092/unibo/amsdottorato/9160.

►

La tesi verte sullo studio morfo-fisiologico e/o patologico del tratto gastrointestinale (GI) di mammiferi. La prima ricerca è basata sull’identificazione, attraverso metodiche di next generation… (more)

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La tesi verte sullo studio morfo-fisiologico e/o patologico del tratto gastrointestinale (GI) di mammiferi. La prima ricerca è basata sull’identificazione, attraverso metodiche di next generation sequencing, di geni coinvolti nell’insorgenza di acalasia sporadica nell’uomo. Tale patologia risulta caratterizzata dalla presenza di anomalie a carico di neuroni del plesso mienterico e cellule interstiziali di Cajal (ICC), che esita in una compromissione della peristalsi del tratto esofageo. É emerso che nei pazienti con acalasia vs controlli il gene C-KIT è down-regolato, mentre INPP4B è up-regolato. Questi geni risultano essere importanti per lo sviluppo e differenziamento neuronale e delle ICC. Il secondo argomento verte allo studio dei processi di de- e fosforilazione della proteina Tau in condizioni fisiologiche e neurodegenerative. Nell’Alzheimer si osserva un accumulo irreversibile di phospho-Tau che induce neurotossicità. Nell’encefalo di animali letargici la proteina Tau viene iperfosforilata in ipotermia, ma defosforila al rientro della normotermia. Inducendo torpore sintetico nel ratto si è osservato che nel sistema nervoso centrale e nel tratto gastrointestinale la phospho-Tau si accumula durante lo stato ipotermico, ma defosforila al ripristino delle temperature fisiologiche. Comprendere le modificazioni associate alla Tau può rappresentare un valido supporto per meglio comprendere quali meccanismi sono alterati in condizioni patologiche. L’ultima parte delle ricerche è stata finalizzata allo studio della distribuzione dei recettori per cannabinoidi quali CB1R, CB2R, GPR55, PPARα, PPARγ, TRPA1 e 5-HT1aR, nel tratto GI di gatto attraverso metodiche immunoistochimiche. Molti autori riportano come l’attivazione di questi recettori svolga un ruolo chiave nei processi infiammatorie ed in generale sulla sensibilità viscerale. La conoscenza della presenza e distribuzione di questi recettori può rappresentare un efficace contributo per future applicazioni in campo farmaceutico/terapeutico. La parte finale dell’elaborato contiene progetti ai quali ho partecipato che esulano dalla tematica principale della tesi ma che hanno esitato in pubblicazioni o partecipazioni a Congressi.

The thesis is focused on a morpho-physiological and pathological study on the gastrointestinal tract (GT) in mammals. The first research talks about the identification, through next generation sequencing, of new genes involved in human sporadic achalasia. This pathology is characterized by abnormalities in myenteric plexus neurons and interstitial cells of Cajal (ICC), that caused esophageal peristalsis impairment. This genetic analysis revealed that achalasia patients vs controls have a downregulation of C-KIT gene and an upregulation of INPP4B. these genes are really important for neuronal and ICC development and differentiation. The second research talks about the processes of de- and phosphorilation of Tau protein in physiological and neurodegenerative conditions. It has been observed that in Alzheimer disease there is an…

Subjects/Keywords: VET/01 Anatomia degli animali domestici

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Stanzani, A. (2020). Aspetti Morfo-Fisiologici dell’Apparato Digerente nei Mammiferi. (Thesis). Università di Bologna. Retrieved from http://amsdottorato.unibo.it/9160/1/stanzani_agnese_tesi.pdf ; Stanzani, Agnese (2020) Aspetti Morfo-Fisiologici dell’Apparato Digerente nei Mammiferi, [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Scienze veterinarie <http://amsdottorato.unibo.it/view/dottorati/DOT507/>, 32 Ciclo. DOI 10.6092/unibo/amsdottorato/9160.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Stanzani, Agnese. “Aspetti Morfo-Fisiologici dell’Apparato Digerente nei Mammiferi.” 2020. Thesis, Università di Bologna. Accessed January 22, 2021. http://amsdottorato.unibo.it/9160/1/stanzani_agnese_tesi.pdf ; Stanzani, Agnese (2020) Aspetti Morfo-Fisiologici dell’Apparato Digerente nei Mammiferi, [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Scienze veterinarie <http://amsdottorato.unibo.it/view/dottorati/DOT507/>, 32 Ciclo. DOI 10.6092/unibo/amsdottorato/9160..

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Stanzani, Agnese. “Aspetti Morfo-Fisiologici dell’Apparato Digerente nei Mammiferi.” 2020. Web. 22 Jan 2021.

Vancouver:

Stanzani A. Aspetti Morfo-Fisiologici dell’Apparato Digerente nei Mammiferi. [Internet] [Thesis]. Università di Bologna; 2020. [cited 2021 Jan 22]. Available from: http://amsdottorato.unibo.it/9160/1/stanzani_agnese_tesi.pdf ; Stanzani, Agnese (2020) Aspetti Morfo-Fisiologici dell’Apparato Digerente nei Mammiferi, [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Scienze veterinarie <http://amsdottorato.unibo.it/view/dottorati/DOT507/>, 32 Ciclo. DOI 10.6092/unibo/amsdottorato/9160..

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Stanzani A. Aspetti Morfo-Fisiologici dell’Apparato Digerente nei Mammiferi. [Thesis]. Università di Bologna; 2020. Available from: http://amsdottorato.unibo.it/9160/1/stanzani_agnese_tesi.pdf ; Stanzani, Agnese (2020) Aspetti Morfo-Fisiologici dell’Apparato Digerente nei Mammiferi, [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Scienze veterinarie <http://amsdottorato.unibo.it/view/dottorati/DOT507/>, 32 Ciclo. DOI 10.6092/unibo/amsdottorato/9160.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Università di Bologna

20. Galiazzo, Giorgia. Characterization of Cannabinoid Receptors in the Peripheral Nervous System and in the Gastrointestinal Tract of Mammals of Veterinary Interest.

Degree: 2020, Università di Bologna

URL: http://amsdottorato.unibo.it/9205/1/galiazzo_giorgia_tesi.pdf ; Galiazzo, Giorgia (2020) Caratterizzazione dei Recettori Cannabinoidi nel Sistema Nervoso Periferico e nel Tratto Gastrointestinale di Mammiferi di Interesse Veterinario, [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Scienze veterinarie <http://amsdottorato.unibo.it/view/dottorati/DOT507/>, 32 Ciclo. DOI 10.6092/unibo/amsdottorato/9205.

► Cannabis has always been used to treat gastrointestinal (GI) symptoms and pain. By considering the beneficial effects obtained in human medicine, the research in veterinary… (more)

▼ Cannabis has always been used to treat gastrointestinal (GI) symptoms and pain. By considering the beneficial effects obtained in human medicine, the research in veterinary medicine has focused on the endocannabinoid system (ECS), developing products to treat inflammatory conditions and nociception. The target of these products are cannabinoid receptors (CBRs), with the two canonical cannabinoid receptor 1 (CB1R) and 2 (CB2R), and different putative cannabinoid receptors, such as G protein coupled receptor 3 (GPR3) and 55 (GPR55), peroxisome proliferator-activated receptors α (PPARα) and γ (PPARγ), transient receptor potential vanniloid (TRPV1) and ankirin (TRPA1), or serotonin receptor (5-HT1a, 5-HT2a or 5-HT3a). The principal aim of this research was to evaluate the role of CBRs in the GI tract and in the dorsal root ganglia (DRGs) of different species. The first chapter focuses on the ECS and its components. The second chapter focuses on the GI tract, describing its structure, the interaction with the ECS and the principal pathologies affecting the species of interest, in which the ECS could be involved. The third chapter focuses on DRGs, describing the structure, the role in neuroinflammation and the interaction with the ECS. The experimental studies are divided in: experimental studies about the GI tract; experimental studies about the DRGs; other experimental studies (focus: endoscopy). The localization of CBRs has been investigated in the GI tract of dog, cat and horse and in canine and equine DRGs. The receptors showed similarities and differences in their distribution, underlining how the ECS modulates its expression adapting to physiological (and pathological) conditions between the different species. This research could provide an anatomical substrate upon which it would be possible to develop preclinical and clinical studies aimed to investigate and possibly support the therapeutic use of non-psychotropic cannabinoid in veterinary medicine.

Subjects/Keywords: VET/01 Anatomia degli animali domestici

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Galiazzo, G. (2020). Characterization of Cannabinoid Receptors in the Peripheral Nervous System and in the Gastrointestinal Tract of Mammals of Veterinary Interest. (Thesis). Università di Bologna. Retrieved from http://amsdottorato.unibo.it/9205/1/galiazzo_giorgia_tesi.pdf ; Galiazzo, Giorgia (2020) Caratterizzazione dei Recettori Cannabinoidi nel Sistema Nervoso Periferico e nel Tratto Gastrointestinale di Mammiferi di Interesse Veterinario, [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Scienze veterinarie <http://amsdottorato.unibo.it/view/dottorati/DOT507/>, 32 Ciclo. DOI 10.6092/unibo/amsdottorato/9205.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Galiazzo, Giorgia. “Characterization of Cannabinoid Receptors in the Peripheral Nervous System and in the Gastrointestinal Tract of Mammals of Veterinary Interest.” 2020. Thesis, Università di Bologna. Accessed January 22, 2021. http://amsdottorato.unibo.it/9205/1/galiazzo_giorgia_tesi.pdf ; Galiazzo, Giorgia (2020) Caratterizzazione dei Recettori Cannabinoidi nel Sistema Nervoso Periferico e nel Tratto Gastrointestinale di Mammiferi di Interesse Veterinario, [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Scienze veterinarie <http://amsdottorato.unibo.it/view/dottorati/DOT507/>, 32 Ciclo. DOI 10.6092/unibo/amsdottorato/9205..

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Galiazzo, Giorgia. “Characterization of Cannabinoid Receptors in the Peripheral Nervous System and in the Gastrointestinal Tract of Mammals of Veterinary Interest.” 2020. Web. 22 Jan 2021.

Vancouver:

Galiazzo G. Characterization of Cannabinoid Receptors in the Peripheral Nervous System and in the Gastrointestinal Tract of Mammals of Veterinary Interest. [Internet] [Thesis]. Università di Bologna; 2020. [cited 2021 Jan 22]. Available from: http://amsdottorato.unibo.it/9205/1/galiazzo_giorgia_tesi.pdf ; Galiazzo, Giorgia (2020) Caratterizzazione dei Recettori Cannabinoidi nel Sistema Nervoso Periferico e nel Tratto Gastrointestinale di Mammiferi di Interesse Veterinario, [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Scienze veterinarie <http://amsdottorato.unibo.it/view/dottorati/DOT507/>, 32 Ciclo. DOI 10.6092/unibo/amsdottorato/9205..

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Galiazzo G. Characterization of Cannabinoid Receptors in the Peripheral Nervous System and in the Gastrointestinal Tract of Mammals of Veterinary Interest. [Thesis]. Università di Bologna; 2020. Available from: http://amsdottorato.unibo.it/9205/1/galiazzo_giorgia_tesi.pdf ; Galiazzo, Giorgia (2020) Caratterizzazione dei Recettori Cannabinoidi nel Sistema Nervoso Periferico e nel Tratto Gastrointestinale di Mammiferi di Interesse Veterinario, [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Scienze veterinarie <http://amsdottorato.unibo.it/view/dottorati/DOT507/>, 32 Ciclo. DOI 10.6092/unibo/amsdottorato/9205.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

21. V. Carollo. DIETARY INTERVENTIONS TO IMPROVE HEALTHY STATUS IN PIG INTENSIVE FARM CONDITIONS: A MORPHO-FUNCTIONAL STUDY OF TARGET ORGANS.

Degree: 2013, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/217365

► The aim of my PhD thesis was to give a contribution in the field of improving pig welfare in different crucial phases of the rearing… (more)

▼ The aim of my PhD thesis was to give a contribution in the field of improving pig welfare in different crucial phases of the rearing period. This on the basis that: i) keeping farm animals healthy is highly recommended for obtaining animal products that are safe for human consumers, and ii) pathologies and difficulties in food animal species growth result in severe economic losses in farming; really, a correct nutritional management may play a key role in animal production, above all when performed utilizing a multidisciplinary approach. We already know that the appropriate dietary composition and amount of nutrients are determinant for an optimal growth of animals. After the banning (2006) from EU countries of the use of chemotherapeutics as growing substances, the interest of scientific researchers for alternative nutritional strategies appears more and more increasing. Various nutritional approaches with different dietary integrations have been tested in the recent years for finding alternatives to the limited use of chemotherapeutics in animal rearing, as well as for enhancing the intrinsic quality of a food product of animal origin. In the last year most literature focus on the so called nutraceuticals. Here I present three assays aimed at evaluating the effects of different dietary supplementations in pigs in two different phases of the intensive farm industries: weaning, which is most often accompanied by acute stress, and growth-finishing period, which is characterized by a long term stress. In all these studies my approach was micro-anatomical, consistent with morpho-functional analyses of the pig alimentary tract. In addition with the description of important structural details, sometimes differently quantified (histometry) in treated animals in comparison with control ones, the used micro-anatomical approach let me to hypothesize in some instances mechanisms of action of the studied dietary interventions. Moreover I have worked to correlate the results obtained with the used micro-anatomical methods with those aimed at evaluating growth performance, thus respecting a multidisciplinary approach, which is useful when approaching the swine that is important both as a food animal species, and a largely used animal model for approaching biomedical themes important for human medicine. In the first assay I studied the way to manage oxidative stress in the growth-finishing phase. The aim of the study was to investigate whether the use of verbascoside (VB), a polyphenolic plant compound, could modulate pig feeding oxidative and/or nitrosative stress in the gut. Eighteen male piglets were assigned to two groups, which were fed with either a control diet (CON) or a diet supplemented with 5 mg/kg of verbascoside (VB) for 166 days. At slaughter, duodenum and jejunum specimens were collected. Immunohistochemistry and Western blot analyses were performed on the samples to evaluate free radical adducts, including acrolein (ACR), 8-hydroxydeoxyguanosine (8-OHdg) and nitrotyrosine (NT). A KRL test was also used to… Advisors/Committee Members: tutor: C. Domeneghini, coordinatore: G. Savoini, DOMENEGHINI, CINZIA MARIA, SAVOINI, GIOVANNI.

Subjects/Keywords: Settore VET/01 - Anatomia degli Animali Domestici; Settore AGR/18 - Nutrizione e Alimentazione Animale

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Carollo, V. (2013). DIETARY INTERVENTIONS TO IMPROVE HEALTHY STATUS IN PIG INTENSIVE FARM CONDITIONS: A MORPHO-FUNCTIONAL STUDY OF TARGET ORGANS. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/217365

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Carollo, V.. “DIETARY INTERVENTIONS TO IMPROVE HEALTHY STATUS IN PIG INTENSIVE FARM CONDITIONS: A MORPHO-FUNCTIONAL STUDY OF TARGET ORGANS.” 2013. Thesis, Università degli Studi di Milano. Accessed January 22, 2021. http://hdl.handle.net/2434/217365.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Carollo, V.. “DIETARY INTERVENTIONS TO IMPROVE HEALTHY STATUS IN PIG INTENSIVE FARM CONDITIONS: A MORPHO-FUNCTIONAL STUDY OF TARGET ORGANS.” 2013. Web. 22 Jan 2021.

Vancouver:

Carollo V. DIETARY INTERVENTIONS TO IMPROVE HEALTHY STATUS IN PIG INTENSIVE FARM CONDITIONS: A MORPHO-FUNCTIONAL STUDY OF TARGET ORGANS. [Internet] [Thesis]. Università degli Studi di Milano; 2013. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/2434/217365.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Carollo V. DIETARY INTERVENTIONS TO IMPROVE HEALTHY STATUS IN PIG INTENSIVE FARM CONDITIONS: A MORPHO-FUNCTIONAL STUDY OF TARGET ORGANS. [Thesis]. Università degli Studi di Milano; 2013. Available from: http://hdl.handle.net/2434/217365

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

22. M.M. Rahman. MORPHOMETRIC AND MOLECULAR STUDIES OF THE CORRELATION BETWEEN CHRONIC MASTITIS AND INFERTILITY IN DAIRY CATTLE.

Degree: 2010, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/150175

► Dairy cow fertility has declined over the past decades even within the recent developments in functional genomics and genetic selections. There is overwhelming evidence that… (more)

▼ Dairy cow fertility has declined over the past decades even within the recent developments in functional genomics and genetic selections. There is overwhelming evidence that increasing genetic merit for milk yield considerably reduces fertility and the strength of negative associations between yield and fertility is obviously towards a rising trend. The deterioration of resistance or increased susceptibility to mastitis as well as reduced fertility in today’s cow, are the examples of undesirable side-effects of the high emphasis on production traits in selection decision. Although, recent studies suggested an association between reproductive failure and mastitis in lactating dairy cows, our understanding of how mastitis affects reproduction is still limited. In this context, we investigated the effects of naturally occurring chronic mastitis on fertility particularly at the level of bovine ovarian stroma with matrix fibers, follicular growth factors, characteristic micro-architecture of stromal vasculature, as well as growth and development of follicles by macroscopic and microscopic morphological as well as immuno-detection based molecular analysis of the ovaries collected at slaughter. Ovaries and milk samples were collected from 74 cows at slaughter. Milk samples from each quarter, were analyzed following National Mastitis Council procedures. Following macroscopic assessment, ovaries of 63 animals were scored as healthy, intermediate and pathological. Based on the presence of major pathogens and somatic cell count results, animals were sorted in 9 groups but only the two extremes were considered for further analysis: uninfected (n = 8) and affected by chronic mastitis (n = 9). Primordial, primary and secondary follicles were counted and scored on similar surface area of ovary sections for each animal (mean ± SD= 5.65 ± 0.25 cm2). They were analyzed with Fisher’s exact test and the association between health status and follicle number was estimated by odds ratios ± confidence limits. Stromal cells with matrix collagen fibres were visualized with Masson trichrome staining. Vasculature area in the ovarian cortex of healthy and mastitic animals was identified using Bandeiraea simplicifolia-I (BSL-I) Lectin. Results were quantified with the dedicated software MacBiophotonics image J, NIH, USA and subsequently analyzed with t-test for statistical significance. Follicles were further characterized by immunostaining with a GDF-9 specific antibody. GDF positive follicular area was measured as well as using a relative scale its fluorescence was semi-quantified. Our results indicate no noticeable macroscopic alteration amongst various macroscopic parameters. Similarly, no (P>0.05) difference was observed between the numbers of primordial and primary follicles in healthy and affected animals. In contrast, the number of secondary follicles was significantly lower in sick animals (Odds ratio 10.50*; p<0.05), indicating a 10 times higher risk for a mastitis animal to have less than 2 secondary follicles per square centimeter. In… Advisors/Committee Members: tutor: Fulvio Gandolfi, GANDOLFI, FULVIO.

Subjects/Keywords: chronic; mastitis; ovary; infertility; correlation; Settore VET/01 - Anatomia degli Animali Domestici

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Rahman, M. (2010). MORPHOMETRIC AND MOLECULAR STUDIES OF THE CORRELATION BETWEEN CHRONIC MASTITIS AND INFERTILITY IN DAIRY CATTLE. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/150175

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Rahman, M.M.. “MORPHOMETRIC AND MOLECULAR STUDIES OF THE CORRELATION BETWEEN CHRONIC MASTITIS AND INFERTILITY IN DAIRY CATTLE.” 2010. Thesis, Università degli Studi di Milano. Accessed January 22, 2021. http://hdl.handle.net/2434/150175.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Rahman, M.M.. “MORPHOMETRIC AND MOLECULAR STUDIES OF THE CORRELATION BETWEEN CHRONIC MASTITIS AND INFERTILITY IN DAIRY CATTLE.” 2010. Web. 22 Jan 2021.

Vancouver:

Rahman M. MORPHOMETRIC AND MOLECULAR STUDIES OF THE CORRELATION BETWEEN CHRONIC MASTITIS AND INFERTILITY IN DAIRY CATTLE. [Internet] [Thesis]. Università degli Studi di Milano; 2010. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/2434/150175.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Rahman M. MORPHOMETRIC AND MOLECULAR STUDIES OF THE CORRELATION BETWEEN CHRONIC MASTITIS AND INFERTILITY IN DAIRY CATTLE. [Thesis]. Università degli Studi di Milano; 2010. Available from: http://hdl.handle.net/2434/150175

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

23. L. DE SANTIS. ORGANIZZAZIONE DEL CITOSCHELETRO, ESPRESSIONE EDISTRIBUZIONE INTRACITOPLASMATICA DI STAUFEN(RNA-BINDING PROTEIN) E CALRETICULINA IN OVOCITIUMANI DURANTE IL PROCESSO MATURATIVO.

Degree: 2013, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/216688

► Before the mammalian oocyte engages in the fertilization process, it must acquire an array of molecular and cellular assets defining its developmental potential. These properties… (more)

▼ Before the mammalian oocyte engages in the fertilization process, it must acquire an array of molecular and cellular assets defining its developmental potential. These properties specify competencies to complete meiosis and initiate mitosis. Meiotic maturation requires the acquisition both of nuclear and cytoplasmic competence and this complex mechanism involves most of the organelles, the cytoskeleton and molecules that are relocated from the nucleus to the cytoplasm. Messenger RNAs of maternal origin are accumulated in the oocyte throughout its growth in the ovary. These transcripts are then shuttled to specific sites of the ooplasm, where local translation is promoted. The oocyte maturation marks the beginning of this phase of activation of maternal transcripts and proteins and involves the "unmasking" of maternal mRNA concomitantly to post-translational changes of stored proteins. This process loosens the stabilization mechanisms that had been imposed during oogenesis. At the end of this route maternal information will be available to the developing embryo. In this scenario, Staufen RNA Binding Protein may play an important role in the light of its function of vector of mRNAs from the nucleus to the sites of translation. Therefore, we hypothesized that Staufen is expressed during oocyte maturation and that the localization of Staufen protein changes during the meiotic transition from GV to MII. We also postulated that the pattern of Staufen distribution reflects its physical interaction with the endoplasmic reticulum, an organelle directly involved in the process of mRNA translation whose relocalization is central to the process of oocyte maturation. To test the above hypotheses, we assessed the expression of Staufen and Calreticulin (CALR), the latter adopted as a marker of the endoplasmic reticulum, in human oocytes at different stages of maturation: GV, MI and MII. The oocytes were obtained from supernumerary material of assisted reproductive cycles and were subjected to polymerase chain reaction in order to investigate the expression of Staufen, CALR and related RNAs. The corresponding protein products were identified by immunofluorescence and confocal laser scanning microscopy. Our findings indicate that Staufen and CALR are constantly expressed and selectively localized during oocyte maturation. The different pattern of Staufen distribution at the GV, MI and MII stages implicates that localization of protein translation is one of the levels at which gene expression is regulated in the maturing oocyte. On the other hand, localization of CALR at the MII stage reveals novel aspects of physical interaction with the MII spindle and suggests a previously unrecognised role of the endoplasmic reticulum in support of cytoskeletal function in the human oocyte. Advisors/Committee Members: tutor: T. Brevini, coordinator: F. Gandolfi, BREVINI, TIZIANA, GANDOLFI, FULVIO.

Subjects/Keywords: oocyte maturation; mRNA; staufen; calreticulin; endoplasmic reticulum; Settore VET/01 - Anatomia degli Animali Domestici

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

SANTIS, L. D. (2013). ORGANIZZAZIONE DEL CITOSCHELETRO, ESPRESSIONE EDISTRIBUZIONE INTRACITOPLASMATICA DI STAUFEN(RNA-BINDING PROTEIN) E CALRETICULINA IN OVOCITIUMANI DURANTE IL PROCESSO MATURATIVO. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/216688

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

SANTIS, L. DE. “ORGANIZZAZIONE DEL CITOSCHELETRO, ESPRESSIONE EDISTRIBUZIONE INTRACITOPLASMATICA DI STAUFEN(RNA-BINDING PROTEIN) E CALRETICULINA IN OVOCITIUMANI DURANTE IL PROCESSO MATURATIVO.” 2013. Thesis, Università degli Studi di Milano. Accessed January 22, 2021. http://hdl.handle.net/2434/216688.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

SANTIS, L. DE. “ORGANIZZAZIONE DEL CITOSCHELETRO, ESPRESSIONE EDISTRIBUZIONE INTRACITOPLASMATICA DI STAUFEN(RNA-BINDING PROTEIN) E CALRETICULINA IN OVOCITIUMANI DURANTE IL PROCESSO MATURATIVO.” 2013. Web. 22 Jan 2021.

Vancouver:

SANTIS LD. ORGANIZZAZIONE DEL CITOSCHELETRO, ESPRESSIONE EDISTRIBUZIONE INTRACITOPLASMATICA DI STAUFEN(RNA-BINDING PROTEIN) E CALRETICULINA IN OVOCITIUMANI DURANTE IL PROCESSO MATURATIVO. [Internet] [Thesis]. Università degli Studi di Milano; 2013. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/2434/216688.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

SANTIS LD. ORGANIZZAZIONE DEL CITOSCHELETRO, ESPRESSIONE EDISTRIBUZIONE INTRACITOPLASMATICA DI STAUFEN(RNA-BINDING PROTEIN) E CALRETICULINA IN OVOCITIUMANI DURANTE IL PROCESSO MATURATIVO. [Thesis]. Università degli Studi di Milano; 2013. Available from: http://hdl.handle.net/2434/216688

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

24. I. Tessaro. LA BOVINA DA LATTE COME MODELLO PER LO STUDIO DEL PRECOCE INVECCHIAMENTO OVARICO: ASPETTI MORFOLOGICI E MOLECOLARI.

Degree: 2010, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/150039

► Several studies in women indicate that precocious ovarian aging is a disorder characterized by an accelerated decline of reproductive potential. We demonstrated that ovaries recovered… (more)

Subjects/Keywords: competenza dell'ovocita; invecchiamento ovarico; stress ossidativo; bovino; oocyte competence; ovarian aging; oxidative stress; cow; Settore VET/01 - Anatomia degli Animali Domestici

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Tessaro, I. (2010). LA BOVINA DA LATTE COME MODELLO PER LO STUDIO DEL PRECOCE INVECCHIAMENTO OVARICO: ASPETTI MORFOLOGICI E MOLECOLARI. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/150039

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Tessaro, I.. “LA BOVINA DA LATTE COME MODELLO PER LO STUDIO DEL PRECOCE INVECCHIAMENTO OVARICO: ASPETTI MORFOLOGICI E MOLECOLARI.” 2010. Thesis, Università degli Studi di Milano. Accessed January 22, 2021. http://hdl.handle.net/2434/150039.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Tessaro, I.. “LA BOVINA DA LATTE COME MODELLO PER LO STUDIO DEL PRECOCE INVECCHIAMENTO OVARICO: ASPETTI MORFOLOGICI E MOLECOLARI.” 2010. Web. 22 Jan 2021.

Vancouver:

Tessaro I. LA BOVINA DA LATTE COME MODELLO PER LO STUDIO DEL PRECOCE INVECCHIAMENTO OVARICO: ASPETTI MORFOLOGICI E MOLECOLARI. [Internet] [Thesis]. Università degli Studi di Milano; 2010. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/2434/150039.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Tessaro I. LA BOVINA DA LATTE COME MODELLO PER LO STUDIO DEL PRECOCE INVECCHIAMENTO OVARICO: ASPETTI MORFOLOGICI E MOLECOLARI. [Thesis]. Università degli Studi di Milano; 2010. Available from: http://hdl.handle.net/2434/150039

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

25. N. Fiandanese. EFFECTS OF IN UTERO AND LACTATIONAL EXPOSURE TO DI(2-ETHYL-HEXYL) PHTHALATE (DEHP) AND POLYCHLORINATED BIPHENYLS (PCBS) IN MICE: REPRODUCTIVE TOXICITY AND MULTIGENERATIONAL TRANSMISSION.

Degree: 2012, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/169562

► Several studies indicate that in utero and peri-natal exposure to some classes of endocrine disruptors induces adverse reproductive effects, but it remains unclear whether such… (more)

▼ Several studies indicate that in utero and peri-natal exposure to some classes of endocrine disruptors induces adverse reproductive effects, but it remains unclear whether such effects may be transmitted to subsequent generations. The present study examined the effects in mice of exposure to di(2-ethyl-hexyl) phthalate (DEHP) or to polychlorinated biphenyls (PCBs) throughout pregnancy and lactation on reproductive health in male and female offspring, at adult age, over three generations. Groups of two to three dams were exposed to increasing doses of contaminants with the diet from gestational day 0.5 until the end of lactation. The doses employed were within the range of environmental exposure levels in humans (DEHP: 0, 0.05, 5, and 500 mg/kg/day; PCBs 101+118: 0, 1, 10, 100 μg/kg/day). In DEHP experiments, treatment of pregnant F0 dams with the 500 mg dose caused complete pregnancy failure, while a slight reduction in litter size in the 5 mg was observed. Male and female F1 offspring born from dams treated with 0.05 and 5 DEHP doses showed, once they reach adult age, significant morphological and functional alterations of the reproductive system. Specifically: i) lower body weight; ii) altered gonad weight (i.e.: lighter testis and heavier ovary) and morphology; iii) reduced germ cells quality; iv) low expression of steroidogenesis and gonadotropin-receptor genes in the gonads; and v) up-regulated gonadotropin subunits gene expression in the pituitary. DEHP exposure altered morphological and reproductive indices in male offspring only in the first generation. Conversely, F2 and F3 female offspring exhibited altered gonad weight and morphology, concomitantly with poor embryo quality, similarly to what observed in F1, thus showing a transgenerational transmission of reproductive adverse effects. Interestingly, also disregulation of selected ovarian and embryonic genes was maintained up to the third generation. In PCBs experiments, treatment did not affect F0 dams’ reproductive outcome. Nevertheless, whole-body PCB burden increased in a dose-dependent manner confirming the effectiveness of the treatment. Furthermore, concentrations at all doses investigated were greater in the offspring than in the dams, confirming that the progeny were exposed as a result of maternal exposure. Pre- and peri-natal exposure to PCBs resulted in male and female offspring showing significant reproductive abnormalities, at adult age. Specifically, compared to controls, they showed reductions in: i) testis weight and seminiferous tubule diameter; ii) sperm viability and developmental capacity; iii) ovary weight; iv) oocyte developmental capacity. Furthermore, F1 ovaries showed a dose-dependent increase in follicular atresia, associated with down-regulation of cyp19a1 and pten mRNA levels. PCBs adverse reproductive effects in females were limited to F1 generation. In contrast, male offspring exhibited reduced sperm viability and altered seminiferous tubule distribution up to the third generation. These results evidence that… Advisors/Committee Members: tutor: Paola Pocar, coordinatore: Fulvio Gandolfi, POCAR, PAOLA, GANDOLFI, FULVIO.

Subjects/Keywords: PCBs; DEHP; transgenerational; EDs; in utero and perinatal exposure; mouse; reproduction; endocrine disruptors; Settore VET/01 - Anatomia degli Animali Domestici

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Fiandanese, N. (2012). EFFECTS OF IN UTERO AND LACTATIONAL EXPOSURE TO DI(2-ETHYL-HEXYL) PHTHALATE (DEHP) AND POLYCHLORINATED BIPHENYLS (PCBS) IN MICE: REPRODUCTIVE TOXICITY AND MULTIGENERATIONAL TRANSMISSION. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/169562

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Fiandanese, N.. “EFFECTS OF IN UTERO AND LACTATIONAL EXPOSURE TO DI(2-ETHYL-HEXYL) PHTHALATE (DEHP) AND POLYCHLORINATED BIPHENYLS (PCBS) IN MICE: REPRODUCTIVE TOXICITY AND MULTIGENERATIONAL TRANSMISSION.” 2012. Thesis, Università degli Studi di Milano. Accessed January 22, 2021. http://hdl.handle.net/2434/169562.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Fiandanese, N.. “EFFECTS OF IN UTERO AND LACTATIONAL EXPOSURE TO DI(2-ETHYL-HEXYL) PHTHALATE (DEHP) AND POLYCHLORINATED BIPHENYLS (PCBS) IN MICE: REPRODUCTIVE TOXICITY AND MULTIGENERATIONAL TRANSMISSION.” 2012. Web. 22 Jan 2021.

Vancouver:

Fiandanese N. EFFECTS OF IN UTERO AND LACTATIONAL EXPOSURE TO DI(2-ETHYL-HEXYL) PHTHALATE (DEHP) AND POLYCHLORINATED BIPHENYLS (PCBS) IN MICE: REPRODUCTIVE TOXICITY AND MULTIGENERATIONAL TRANSMISSION. [Internet] [Thesis]. Università degli Studi di Milano; 2012. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/2434/169562.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Fiandanese N. EFFECTS OF IN UTERO AND LACTATIONAL EXPOSURE TO DI(2-ETHYL-HEXYL) PHTHALATE (DEHP) AND POLYCHLORINATED BIPHENYLS (PCBS) IN MICE: REPRODUCTIVE TOXICITY AND MULTIGENERATIONAL TRANSMISSION. [Thesis]. Università degli Studi di Milano; 2012. Available from: http://hdl.handle.net/2434/169562

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

26. D. Corbani. MOLECULAR DETERMINANTS OF MAMMALIAN FERTILITY: ROLE OF PROGESTERONE RECEPTOR MEMBRANE COMPONENT 1 (PGRMC1).

Degree: 2013, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/216690

► Reproductive efficiency in dairy cows is decreasing worldwide. The root cause of the declining fertility is probably a combination of a variety of physiological and… (more)

▼ Reproductive efficiency in dairy cows is decreasing worldwide. The root cause of the declining fertility is probably a combination of a variety of physiological and management factors that have an additive effect on reproductive efficiency. These factors include increasing in milk production and herd size, greater use of confinement housing, labor shortages and higher inbreeding percentages. In addition, the reproductive physiology of dairy cattle has also changed in response to genetic selection for milk production. Compared with traditional dairy cows, modern dairy cows have longer intervals to first ovulation, a higher incidence of anestrus and abnormal luteal phases, lower blood progesterone and IGF-I concentrations, higher incidence of multiple ovulations and twinning rates as well as greater embryonic loss. Declining fertility represents an obstacle in maintaining profitability of dairy farms. It has been estimated that a slight increase in pregnancy rates results in a significant increase in profitability that could make the difference in whether a family farm remains in operation. These findings suggest that defining the factors and mechanisms that contribute to oocyte and embryo quality is essential for improving female fertility. In particular, basic knowledge of which proteins within the oocyte regulate meiosis, oocyte fertilizability and developmental potential would be advantageous. Starting from these observations, our studies were conducted to test the hypothesis that PGRMC1 is one of the key factors that regulate mammalian oocyte quality and therefore female fertility. Initial indications that PGRMC1 participates in progesterone signaling in the reproductive system come from studies in which PGRMC1 expression was silenced using siRNA in ovarian cells. These experiments demonstrated that Progesterone’s ability to inhibit ovarian cells from undergoing apoptosis in vitro is dependent on PGRMC1, indicating that PGRMC1 plays an essential role in promoting the survival of ovarian cell in vitro. This led us to start our investigations on the function of PGRMC1 in bovine fertility. Initial experiments were conducted to determine the presence and localization of PGRMC1 in various compartments of the bovine female reproductive organs, during the follicular and luteal phases of the estrous cycle. Importantly, these studies revealed the presence of PGRMC1 in the nucleus of bovine oocytes. Further studies revealed that PGRMC1 is present in both GV- and MII-stage oocyte, is associated with male and female pronuclei in the zygote and is highly expressed in the blastocysts, with typical localization at each of these stages. Since fertilization and embryonic development are mainly dependent on the completion of oocyte maturation, we focused our attention on PGRMC1’s role during this important step. Our localization as well as our functional data suggest an important role of PGRMC1 in oocyte maturation that may be specifically related to the mechanism by which chromosomes segregate and the first polar body… Advisors/Committee Members: tutor: A.M. Luciano, coordinator: F. Gandolfi, LUCIANO, ALBERTO MARIA, GANDOLFI, FULVIO.

Subjects/Keywords: PGRMC1; AURKB; oocyte; meiosis; premature ovarian failure; antral follicle count; ovary; infertility; Settore VET/01 - Anatomia degli Animali Domestici

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APA (6^th Edition):

Corbani, D. (2013). MOLECULAR DETERMINANTS OF MAMMALIAN FERTILITY: ROLE OF PROGESTERONE RECEPTOR MEMBRANE COMPONENT 1 (PGRMC1). (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/216690

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Corbani, D.. “MOLECULAR DETERMINANTS OF MAMMALIAN FERTILITY: ROLE OF PROGESTERONE RECEPTOR MEMBRANE COMPONENT 1 (PGRMC1).” 2013. Thesis, Università degli Studi di Milano. Accessed January 22, 2021. http://hdl.handle.net/2434/216690.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Corbani, D.. “MOLECULAR DETERMINANTS OF MAMMALIAN FERTILITY: ROLE OF PROGESTERONE RECEPTOR MEMBRANE COMPONENT 1 (PGRMC1).” 2013. Web. 22 Jan 2021.

Vancouver:

Corbani D. MOLECULAR DETERMINANTS OF MAMMALIAN FERTILITY: ROLE OF PROGESTERONE RECEPTOR MEMBRANE COMPONENT 1 (PGRMC1). [Internet] [Thesis]. Università degli Studi di Milano; 2013. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/2434/216690.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Corbani D. MOLECULAR DETERMINANTS OF MAMMALIAN FERTILITY: ROLE OF PROGESTERONE RECEPTOR MEMBRANE COMPONENT 1 (PGRMC1). [Thesis]. Università degli Studi di Milano; 2013. Available from: http://hdl.handle.net/2434/216690

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

27. S. Maffei. OVARIAN TISSUE CRYOPRESERVATION FOR THE SAFEGUARD OF FEMALE FERTILITY.

Degree: 2014, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/230860

► Ovarian tissue cryobanking is considered an available method for preserving female fertility and represents the best option in young pre-pubertal cancer patients. Cryopreservation can be… (more)

Subjects/Keywords: Settore VET/01 - Anatomia degli Animali Domestici

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Maffei, S. (2014). OVARIAN TISSUE CRYOPRESERVATION FOR THE SAFEGUARD OF FEMALE FERTILITY. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/230860

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Maffei, S.. “OVARIAN TISSUE CRYOPRESERVATION FOR THE SAFEGUARD OF FEMALE FERTILITY.” 2014. Thesis, Università degli Studi di Milano. Accessed January 22, 2021. http://hdl.handle.net/2434/230860.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Maffei, S.. “OVARIAN TISSUE CRYOPRESERVATION FOR THE SAFEGUARD OF FEMALE FERTILITY.” 2014. Web. 22 Jan 2021.

Vancouver:

Maffei S. OVARIAN TISSUE CRYOPRESERVATION FOR THE SAFEGUARD OF FEMALE FERTILITY. [Internet] [Thesis]. Università degli Studi di Milano; 2014. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/2434/230860.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Maffei S. OVARIAN TISSUE CRYOPRESERVATION FOR THE SAFEGUARD OF FEMALE FERTILITY. [Thesis]. Università degli Studi di Milano; 2014. Available from: http://hdl.handle.net/2434/230860

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

28. M.E. Andreis. THE BONE TISSUE: MULTIDISCIPLINARY CHARACTERISATION IN EXPERIMENTAL ANIMALS AND SKELETAL DEVELOPMENT IN DOGS.

Degree: 2019, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/627779

► Animals are considered sentient beings by law and the notion of animal welfare, both physical and psychological, is well-established (“Five Freedoms”). As a result, regulation… (more)

▼ Animals are considered sentient beings by law and the notion of animal welfare, both physical and psychological, is well-established (“Five Freedoms”). As a result, regulation on animal testing puts researchers through highly regulated and restrained protocols (to reduce the number of animals included in in vivo experiments and replace them with alternative procedures whenever possible. This project revolves around the study of the bone tissue in different species, grounding on the idea of cadaver “recycle” applied to bone-related research. The bone, in fact, is particularly easy to study in cadavers since it suffers the early consequences of post-mortal decay to a lesser extent compared to other tissues. The bone is a highly specialized connective tissue characterized by a mineralized extracellular matrix, specifically designed for structural, mechanical and metabolic functions. Despite general shared features, interspecific differences exist in bone microscopic organisation, which have not yet been completely elucidated. Because of its peculiarities, a comprehensive evaluation of the bone includes several different techniques, such as histology, densitometry and biomechanics. Histologic techniques are particularly common, especially when it is necessary to evaluate tissue biocompatibility and integration in animal models of bone healing and regeneration. However, due to highly mineralized nature of its matrix, several problems arise during bone processing, especially during decalcification (the removal of mineral from the bone while preserving all the essential microscopic elements and tissue antigenicity), a process that is yet far from being standardized, but is necessary to prepare paraffin-embedded specimens. The first two aims of this project were the definition of species-specific and site-specific guidelines for bone specimens’ decalcification and the characterization of swine and ovine bone tissue with a multidisciplinary approach. Basic protocols for trabecular bone decalcification in different species with different solutions were provided, showing that excellent morphologic results can be achieved with solution containing strong acids in rodents, sheep and pig. As a general rule, hydrochloric acid solution proved to be too aggressive, as previously reported. On the other hand, the combined use of hydrochloric acid and formic acid proved to be a very good compromise, shortening experimental time and preserving morphology. Finally, a combination of citric acid formic acid provided excellent morphology, but at the expenses of decalcification time. Collagen I was preserved in swine, canine and ovine samples that proved to have good/excellent morphology, while it wasn’t preserved in rats or mice, despite their excellent morphology. A multidisciplinary approach to the study of the bone in pigs and sheep was developed, evaluating several different parameters with different techniques on the same samples, also providing standard reference parameters about swine and ovine bone histology, densitometry and… Advisors/Committee Members: supervisor: S.C. Modina, co-supervisor: M. Di Giancamillo, phd coordinator: F. Gandolfi, MODINA, SILVIA CLOTILDE, MODINA, SILVIA CLOTILDE, DI GIANCAMILLO, MAURO, GANDOLFI, FULVIO.

Subjects/Keywords: bone; dog; puppy; pig; sheep; decalcification; cartilage canals; skeletal development; quadriceps contracture; Settore VET/01 - Anatomia degli Animali Domestici

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Andreis, M. (2019). THE BONE TISSUE: MULTIDISCIPLINARY CHARACTERISATION IN EXPERIMENTAL ANIMALS AND SKELETAL DEVELOPMENT IN DOGS. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/627779

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Andreis, M.E.. “THE BONE TISSUE: MULTIDISCIPLINARY CHARACTERISATION IN EXPERIMENTAL ANIMALS AND SKELETAL DEVELOPMENT IN DOGS.” 2019. Thesis, Università degli Studi di Milano. Accessed January 22, 2021. http://hdl.handle.net/2434/627779.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Andreis, M.E.. “THE BONE TISSUE: MULTIDISCIPLINARY CHARACTERISATION IN EXPERIMENTAL ANIMALS AND SKELETAL DEVELOPMENT IN DOGS.” 2019. Web. 22 Jan 2021.

Vancouver:

Andreis M. THE BONE TISSUE: MULTIDISCIPLINARY CHARACTERISATION IN EXPERIMENTAL ANIMALS AND SKELETAL DEVELOPMENT IN DOGS. [Internet] [Thesis]. Università degli Studi di Milano; 2019. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/2434/627779.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Andreis M. THE BONE TISSUE: MULTIDISCIPLINARY CHARACTERISATION IN EXPERIMENTAL ANIMALS AND SKELETAL DEVELOPMENT IN DOGS. [Thesis]. Università degli Studi di Milano; 2019. Available from: http://hdl.handle.net/2434/627779

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Università degli Studi di Padova

29. Roncon, Giulia. Studio morfologico e funzionale del claustro nel tursiope.

Degree: 2013, Università degli Studi di Padova

URL: http://tesi.cab.unipd.it/43481/1/Roncon_Giulia.pdf

Subjects/Keywords: VET/01 Anatomia degli animali domestici

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Roncon, G. (2013). Studio morfologico e funzionale del claustro nel tursiope. (Thesis). Università degli Studi di Padova. Retrieved from http://tesi.cab.unipd.it/43481/1/Roncon_Giulia.pdf

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Roncon, Giulia. “Studio morfologico e funzionale del claustro nel tursiope.” 2013. Thesis, Università degli Studi di Padova. Accessed January 22, 2021. http://tesi.cab.unipd.it/43481/1/Roncon_Giulia.pdf.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Roncon, Giulia. “Studio morfologico e funzionale del claustro nel tursiope.” 2013. Web. 22 Jan 2021.

Vancouver:

Roncon G. Studio morfologico e funzionale del claustro nel tursiope. [Internet] [Thesis]. Università degli Studi di Padova; 2013. [cited 2021 Jan 22]. Available from: http://tesi.cab.unipd.it/43481/1/Roncon_Giulia.pdf.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Roncon G. Studio morfologico e funzionale del claustro nel tursiope. [Thesis]. Università degli Studi di Padova; 2013. Available from: http://tesi.cab.unipd.it/43481/1/Roncon_Giulia.pdf

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Università di Bologna

30. Paradisi, Michela. Neural stem cells: studio in vivo e in vitro nel cervello adulto.

Degree: 2007, Università di Bologna

URL: http://amsdottorato.unibo.it/492/1/TesiMichelaParadisi.pdf ; Paradisi, Michela (2007) Neural stem cells: studio in vivo e in vitro nel cervello adulto, [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Applicazioni biotecnologiche in neuromorfofisiologia <http://amsdottorato.unibo.it/view/dottorati/DOT201/>, 19 Ciclo. DOI 10.6092/unibo/amsdottorato/492.

Subjects/Keywords: VET/01 Anatomia degli animali domestici

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Paradisi, M. (2007). Neural stem cells: studio in vivo e in vitro nel cervello adulto. (Thesis). Università di Bologna. Retrieved from http://amsdottorato.unibo.it/492/1/TesiMichelaParadisi.pdf ; Paradisi, Michela (2007) Neural stem cells: studio in vivo e in vitro nel cervello adulto, [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Applicazioni biotecnologiche in neuromorfofisiologia <http://amsdottorato.unibo.it/view/dottorati/DOT201/>, 19 Ciclo. DOI 10.6092/unibo/amsdottorato/492.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Paradisi, Michela. “Neural stem cells: studio in vivo e in vitro nel cervello adulto.” 2007. Thesis, Università di Bologna. Accessed January 22, 2021. http://amsdottorato.unibo.it/492/1/TesiMichelaParadisi.pdf ; Paradisi, Michela (2007) Neural stem cells: studio in vivo e in vitro nel cervello adulto, [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Applicazioni biotecnologiche in neuromorfofisiologia <http://amsdottorato.unibo.it/view/dottorati/DOT201/>, 19 Ciclo. DOI 10.6092/unibo/amsdottorato/492..

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Paradisi, Michela. “Neural stem cells: studio in vivo e in vitro nel cervello adulto.” 2007. Web. 22 Jan 2021.

Vancouver:

Paradisi M. Neural stem cells: studio in vivo e in vitro nel cervello adulto. [Internet] [Thesis]. Università di Bologna; 2007. [cited 2021 Jan 22]. Available from: http://amsdottorato.unibo.it/492/1/TesiMichelaParadisi.pdf ; Paradisi, Michela (2007) Neural stem cells: studio in vivo e in vitro nel cervello adulto, [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Applicazioni biotecnologiche in neuromorfofisiologia <http://amsdottorato.unibo.it/view/dottorati/DOT201/>, 19 Ciclo. DOI 10.6092/unibo/amsdottorato/492..

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Paradisi M. Neural stem cells: studio in vivo e in vitro nel cervello adulto. [Thesis]. Università di Bologna; 2007. Available from: http://amsdottorato.unibo.it/492/1/TesiMichelaParadisi.pdf ; Paradisi, Michela (2007) Neural stem cells: studio in vivo e in vitro nel cervello adulto, [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Applicazioni biotecnologiche in neuromorfofisiologia <http://amsdottorato.unibo.it/view/dottorati/DOT201/>, 19 Ciclo. DOI 10.6092/unibo/amsdottorato/492.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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