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Universidade Federal de Mato Grosso do Sul
1.
Pereira, Edy Firmina.
Distribuição geográfica, epidemiologia e clínica de casos de PCM causada por Paracoccidioides brasiliensis e P. lutzii no Estado de Mato Grosso do Sul
.
Degree: 2015, Universidade Federal de Mato Grosso do Sul
URL: http://repositorio.cbc.ufms.br:8080/jspui/handle/123456789/2913 
► Paracoccidioidomicose (PCM) é uma micose sistêmica endêmica na América Latina causada por um fungo termo-dimórfico da família Ajellomicetaceae e gênero Paracoccidiodes. Até pouco tempo acreditava-se…
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▼ Paracoccidioidomicose (PCM) é uma micose sistêmica endêmica na América Latina causada por um fungo termo-dimórfico da família Ajellomicetaceae e gênero Paracoccidiodes. Até pouco tempo acreditava-se ser o P. brasiliensis a única espécie envolvida na etiologia da PCM, até que, em 2009, foi identificada uma espécie denominada P. lutziia partir de isolados de pacientes com a doença, predominantemente na região Centro-Oeste do Brasil. A identificação das espécies do Paracoccidioidestem sido feitas baseando-seno sequenciamento genético do fungo obtido de cultivos. A diferenciação da PCM causada pelo P. brasilensis do P. lutzii pode ser obtida por pesquisa de anticorpos no soro de pacientes, utilizando-se antígenos específicos das duas espécies.A distribuição geográfica do P. lutzii e os aspectos epidemiológicos e clínicos da PCM associados a essa espécie ainda são pouco conhecidos.Detectando a ocorrência de PCM causada peloParacoccidioideslutzii em um Centro de Referência de Mato Grosso do Sul bem como comparar os aspectos clínicos e epidemiológicos da PCM causada pelas duas espécies.Foramselecionadossoros de 82 pacientes com PCM atendidos no Hospital Universitário da Universidade Federal de Mato Grosso do Sul, entre os anos de 2008 e 2012. Foi realizada Imunodifusão com a gp43, com exoantígeno de isolado B339 (ExoPb) e com antígenos obtidos de superfície celular CellFreeAntigen de isolado de P. lutzii (CFA Pl). Sendo considerado caso de PCM por P. lutzii aquele cujo soro reagiu contra CFA-Pl e não reagiu contra gp43.A procedência, dados clínicos, demográficos e epidemiológicos de casos de PCM causada por P. lutzii foram comparados com os casos por P.brasiliensis. Foram identificados 16 casos (19,75%) de PCM por P. lutzii e 65 casos (80,25%) de PCM por P. brasiliensis. A ID com ExoPb foi reagente em 83,1% dos 65 casos de P. brasiliensis e 31,3% dos 16 casos P.lutzii. A ID com CFA Pl resultou falso-positiva em 21,5% dos casos de PCM por P. brasiliensis. Os casos de P. lutzii procederam de diferentes municípios do Estado e não foram observadas diferenças demográficas e clínicas entre os casos de PCM por P.lutzii e PCM por P. brasiliensis. Um quinto dos pacientes atendidos no local do estudo apresentaram PCM causada por P. lutzii. O diagnóstico sorológico deve ser realizado com diferentes antígenos para a identificação de casos causados pelas diferentes espécies. Parece não haver diferenças clínicas entre a PCM causada pelas duas espécies.
Advisors/Committee Members: Paniago, Anamaria Miranda Mello (advisor).
Subjects/Keywords: Paracoccidioidomicose;
Sorologia;
Paracoccidioidomycosis;
Serology
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APA (6th Edition):
Pereira, E. F. (2015). Distribuição geográfica, epidemiologia e clínica de casos de PCM causada por Paracoccidioides brasiliensis e P. lutzii no Estado de Mato Grosso do Sul
. (Masters Thesis). Universidade Federal de Mato Grosso do Sul. Retrieved from http://repositorio.cbc.ufms.br:8080/jspui/handle/123456789/2913
Chicago Manual of Style (16th Edition):
Pereira, Edy Firmina. “Distribuição geográfica, epidemiologia e clínica de casos de PCM causada por Paracoccidioides brasiliensis e P. lutzii no Estado de Mato Grosso do Sul
.” 2015. Masters Thesis, Universidade Federal de Mato Grosso do Sul. Accessed March 08, 2021.
http://repositorio.cbc.ufms.br:8080/jspui/handle/123456789/2913.
MLA Handbook (7th Edition):
Pereira, Edy Firmina. “Distribuição geográfica, epidemiologia e clínica de casos de PCM causada por Paracoccidioides brasiliensis e P. lutzii no Estado de Mato Grosso do Sul
.” 2015. Web. 08 Mar 2021.
Vancouver:
Pereira EF. Distribuição geográfica, epidemiologia e clínica de casos de PCM causada por Paracoccidioides brasiliensis e P. lutzii no Estado de Mato Grosso do Sul
. [Internet] [Masters thesis]. Universidade Federal de Mato Grosso do Sul; 2015. [cited 2021 Mar 08].
Available from: http://repositorio.cbc.ufms.br:8080/jspui/handle/123456789/2913.
Council of Science Editors:
Pereira EF. Distribuição geográfica, epidemiologia e clínica de casos de PCM causada por Paracoccidioides brasiliensis e P. lutzii no Estado de Mato Grosso do Sul
. [Masters Thesis]. Universidade Federal de Mato Grosso do Sul; 2015. Available from: http://repositorio.cbc.ufms.br:8080/jspui/handle/123456789/2913
2.
Janet, Sunohara-Neilson.
Investigation of Leporid herpesvirus 4, an Emerging Pathogen of Rabbits: Infection and Prevalence Studies.
Degree: Doctor of Veterinary Science, Department of Pathobiology, 2014, University of Guelph
URL: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/7792
► Leporid herpesvirus 4 (LeHV-4) is a recently identified alphaherpesvirus that causes lethal respiratory disease in rabbits. Diagnosis has been dependent on the observation of distinctive…
(more)
▼ Leporid herpesvirus 4 (LeHV-4) is a recently identified alphaherpesvirus that causes lethal respiratory disease in rabbits. Diagnosis has been dependent on the observation of distinctive intranuclear inclusion bodies in affected tissues. The objectives of this body of work were to describe the course of infection in laboratory rabbits, develop a serological test for the detection of antibodies to LeHV-4, and survey Ontario commercial meat rabbits and pet rabbits for LeHV-4 antibody prevalence. Based on the results of an initial dose-range finding pilot study, 22 New Zealand white rabbits were inoculated intranasally with LeHV-4 and monitored for 22 days post-infection (dpi). Clinical signs of infection, including dyspnea, serous oculonasal discharge, pyrexia and weight loss, were evident from 2 to 7 dpi. LeHV-4 was isolated from nasal secretions between 2 and 10 dpi. Gross and microscopic pathology was evaluated and suppurative necrohemorrhagic pneumonia and splenic necrosis were the major findings at peak infection (5 to 7 dpi), at which time eosinophilic herpetic inclusions were present in nasal mucosa, skin, spleen, and lung. Virus neutralization (VN) assay demonstrated serum antibodies starting at 11 dpi and persisting until the study end (22 dpi). Polyclonal antibodies generated to inactivated virus in laboratory rabbits were neutralizing with low titres of 1:64 or less. Serum samples were obtained from 225 commercial meat rabbits and 24 pet rabbits and all were negative for LeHV-4 antibodies by VN assay. To increase the safety of the assay while expediting rabbit screening for antibody to LeHV-4, an indirect enzyme-linked immunosorbant assay (ELISA) was developed using glycoprotein G (gG) as target antigen. Recombinant LeHV-4 gG was generated using a baculovirus expression system. The presence of gG in infected Spodoptera frugiperda (Sf-21) cells was confirmed by Western blot analysis. With this infected cell lysate as target antigen, the indirect ELISA had 100% sensitivity and 99% specificity, and can be used to screen rabbits for exposure to LeHV-4. In addition to characterizing the clinicopathological course of the disease in rabbits, this body of work has demonstrated that LeHV-4 is an uncommon disease, and a latent reservoir is unlikely in Ontario domestic rabbits.
Advisors/Committee Members: Turner, Patricia (advisor).
Subjects/Keywords: rabbits; herpesvirus; serology; pathology
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APA (6th Edition):
Janet, S. (2014). Investigation of Leporid herpesvirus 4, an Emerging Pathogen of Rabbits: Infection and Prevalence Studies. (Doctoral Dissertation). University of Guelph. Retrieved from https://atrium.lib.uoguelph.ca/xmlui/handle/10214/7792
Chicago Manual of Style (16th Edition):
Janet, Sunohara-Neilson. “Investigation of Leporid herpesvirus 4, an Emerging Pathogen of Rabbits: Infection and Prevalence Studies.” 2014. Doctoral Dissertation, University of Guelph. Accessed March 08, 2021.
https://atrium.lib.uoguelph.ca/xmlui/handle/10214/7792.
MLA Handbook (7th Edition):
Janet, Sunohara-Neilson. “Investigation of Leporid herpesvirus 4, an Emerging Pathogen of Rabbits: Infection and Prevalence Studies.” 2014. Web. 08 Mar 2021.
Vancouver:
Janet S. Investigation of Leporid herpesvirus 4, an Emerging Pathogen of Rabbits: Infection and Prevalence Studies. [Internet] [Doctoral dissertation]. University of Guelph; 2014. [cited 2021 Mar 08].
Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/7792.
Council of Science Editors:
Janet S. Investigation of Leporid herpesvirus 4, an Emerging Pathogen of Rabbits: Infection and Prevalence Studies. [Doctoral Dissertation]. University of Guelph; 2014. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/7792
Kwame Nkrumah University of Science and Technology
3.
King, S.; Adjei-Asante, K.; Appiah, L.; Adinku, D.; Phillips, Richard O.
Antibody screening tests variably overestimate the prevalence of hepatitis C virus infection among HIV-infected adults in Ghana.
Degree: 2014, Kwame Nkrumah University of Science and Technology
URL: http://dspace.knust.edu.gh:8080/jspui/handle/123456789/11876
► HIV coinfection with HCV has been poorly studied in sub-Saharan Africa, and the reliability of available seroprevalence estimates remains uncertain. The study aim was to…
(more)
▼ HIV coinfection with HCV has been poorly studied
in sub-Saharan Africa, and the reliability of available
seroprevalence estimates remains uncertain. The study aim
was to determine HCV RNA prevalence in HIV-infected
subjects receiving care in Kumasi, Ghana, and relate the
findings to HCV antibody detection. From a population of
1520 HIV-infected adults, all HBsAg-positive subjects
(n = 236) and a random subset of HBsAg-negative subject
(n = 172) were screened for HCV RNA using pooled
plasma; positive samples were genotyped by core and
NS5B sequencing. HCV antibodies were detected by three
commercial screening assays and confirmed by the line
immunoassay. HCV RNA was detected in 4/408 subjects
(1.0%, 95% confidence interval 0.0–1.9%), comprising 3/
236 (1.3%; 0.0–2.8%) HBsAg-positive and 1/172 (0.6%;
0.0–1.8%) HBsAg-negative subjects. HCV RNA-positive
subjects showed reactivity in all three antibody screening
assays. Among HCV RNA-negative subjects, 5/67 (7.5%),
5/67 (7.5%) and 19/67 (28.4%) showed antibody reactivity
by each screening assay, respectively, including two
(3.0%) with reactivity by all three assays. Only one sample
(1.5%) had confirmed antibody reactivity by line immunoassay
indicating past HCV infection. HCV-positive subjects
(three males, two females) were aged 30–46 years, by
questionnaire-based interview reported surgical procedures
and blood transfusion as risk factors for infection. HCV
genotypes were 2 (subtypes 2j, 2l, 2k/unassigned) and 1
(subtype unassigned). Without further testing, HCV antibody
screening assays variably overestimated HCV prevalence
among HIV-infected subjects in Ghana. These
findings inform the interpretation of previous seroprevalence
estimates based upon screening assays alone.
An article published by John Wiley & Sons Ltd and available at doi:10.1111/jvh.12354
KNUST
Subjects/Keywords: Africa; antibody; genotype; RNA; serology
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APA (6th Edition):
King, S.; Adjei-Asante, K.; Appiah, L.; Adinku, D.; Phillips, R. O. (2014). Antibody screening tests variably overestimate the prevalence of hepatitis C virus infection among HIV-infected adults in Ghana. (Thesis). Kwame Nkrumah University of Science and Technology. Retrieved from http://dspace.knust.edu.gh:8080/jspui/handle/123456789/11876
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
King, S.; Adjei-Asante, K.; Appiah, L.; Adinku, D.; Phillips, Richard O. “Antibody screening tests variably overestimate the prevalence of hepatitis C virus infection among HIV-infected adults in Ghana.” 2014. Thesis, Kwame Nkrumah University of Science and Technology. Accessed March 08, 2021.
http://dspace.knust.edu.gh:8080/jspui/handle/123456789/11876.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
King, S.; Adjei-Asante, K.; Appiah, L.; Adinku, D.; Phillips, Richard O. “Antibody screening tests variably overestimate the prevalence of hepatitis C virus infection among HIV-infected adults in Ghana.” 2014. Web. 08 Mar 2021.
Vancouver:
King, S.; Adjei-Asante, K.; Appiah, L.; Adinku, D.; Phillips RO. Antibody screening tests variably overestimate the prevalence of hepatitis C virus infection among HIV-infected adults in Ghana. [Internet] [Thesis]. Kwame Nkrumah University of Science and Technology; 2014. [cited 2021 Mar 08].
Available from: http://dspace.knust.edu.gh:8080/jspui/handle/123456789/11876.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
King, S.; Adjei-Asante, K.; Appiah, L.; Adinku, D.; Phillips RO. Antibody screening tests variably overestimate the prevalence of hepatitis C virus infection among HIV-infected adults in Ghana. [Thesis]. Kwame Nkrumah University of Science and Technology; 2014. Available from: http://dspace.knust.edu.gh:8080/jspui/handle/123456789/11876
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Edinburgh
4.
Guido, Stefano.
Development of diagnostic tests for the detection of Neospora caninum infected cattle.
Degree: PhD, 2018, University of Edinburgh
URL: http://hdl.handle.net/1842/31168
► The protozoan parasite Neospora caninum is among the most frequently diagnosed infectious causes of bovine abortion thus causing significant economic losses, production inefficiency and welfare…
(more)
▼ The protozoan parasite Neospora caninum is among the most frequently diagnosed infectious causes of bovine abortion thus causing significant economic losses, production inefficiency and welfare concern to the cattle sector worldwide. The control of bovine neosporosis relies on management techniques within which the identification of infected animals by serological testing plays a key role. However, the reliable diagnosis of the disease is hindered by the complex host-parasite interactions and the intrinsic limitations of the serological diagnostic tools currently available; as a result, some infected animals may not be detected. At the herd, regional and national levels, this can potentially undermine efforts for the control of the disease. The work presented in this thesis was undertaken to further investigate avenues to improve the diagnosis of N. caninum infection in cattle. It has been shown previously that recombinant antigens expressed by the rapidly proliferating tachyzoite stage and the slowly multiplying bradyzoite stage of N. caninum can be successfully employed for the detection of specific antibody responses during acute and persistent infections respectively. Following the establishment of persistent infections, sustained by the bradyzoite stage, the antibody levels against the tachyzoite stage may decline below the detection limits of currently available diagnostic tests which are exclusively based on antigens expressed by the tachyzoite. Consequently, the use of bradyzoite antigens for the development of serological diagnostic tests, may enhance the identification of infected animals. Novel antigens putatively expressed by the quiescent bradyzoite stage of N. caninum have been identified, expressed as recombinant proteins and assayed for the detection of specific antibodies. The recognition of recombinant tNcSRS12A-B and tNcSRS44- A by specific antibodies in sera from persistently N. caninum-infected cattle suggested that these proteins could be used for the detection of persistently infected animals. Indirect ELISAs based on previously characterised N. caninum antigens, such as the tachyzoite surface protein rNcSRS2, the immunodominant dense granule protein rNcGRA7 and the bradyzoite specific surface antigens rNcSAG4, rNcBSR4 and rNcSRS9, as well as a commercial test using whole tachyzoite lysate as antigenic preparation, were evaluated within a cross-sectional study to estimate the seroprevalence of bovine neosporosis in British dairy cattle. Moderate, but not high, agreement was found amongst the tests based on whole tachyzoite lysate, rNcSRS2 and rNcGRA7, and amongst the bradyzoite-specific antigen-based iELISAs. In contrast, only slight agreement was observed when each test detecting antibody responses indicative of acute infection (whole tachyzoite lysate, rNcSRS2 and rNcGRA7) was compared with each test detecting antibody responses indicative of persistent infection (bradyzoite-specific antigen-based iELISAs). Most N. caninum antibody-positive cattle samples showed detectable antibodies only…
Subjects/Keywords: 636.2; Neospora; diagnosis; control; serology
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APA (6th Edition):
Guido, S. (2018). Development of diagnostic tests for the detection of Neospora caninum infected cattle. (Doctoral Dissertation). University of Edinburgh. Retrieved from http://hdl.handle.net/1842/31168
Chicago Manual of Style (16th Edition):
Guido, Stefano. “Development of diagnostic tests for the detection of Neospora caninum infected cattle.” 2018. Doctoral Dissertation, University of Edinburgh. Accessed March 08, 2021.
http://hdl.handle.net/1842/31168.
MLA Handbook (7th Edition):
Guido, Stefano. “Development of diagnostic tests for the detection of Neospora caninum infected cattle.” 2018. Web. 08 Mar 2021.
Vancouver:
Guido S. Development of diagnostic tests for the detection of Neospora caninum infected cattle. [Internet] [Doctoral dissertation]. University of Edinburgh; 2018. [cited 2021 Mar 08].
Available from: http://hdl.handle.net/1842/31168.
Council of Science Editors:
Guido S. Development of diagnostic tests for the detection of Neospora caninum infected cattle. [Doctoral Dissertation]. University of Edinburgh; 2018. Available from: http://hdl.handle.net/1842/31168
Montana State University
5.
Ericson, John Edward.
Serology as a technique to detect major genetic differences in the genus Agropyron Gaertn.
Degree: PhD, College of Agriculture, 1968, Montana State University
URL: https://scholarworks.montana.edu/xmlui/handle/1/4186
Subjects/Keywords: Serology.; Grasses.
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APA (6th Edition):
Ericson, J. E. (1968). Serology as a technique to detect major genetic differences in the genus Agropyron Gaertn. (Doctoral Dissertation). Montana State University. Retrieved from https://scholarworks.montana.edu/xmlui/handle/1/4186
Chicago Manual of Style (16th Edition):
Ericson, John Edward. “Serology as a technique to detect major genetic differences in the genus Agropyron Gaertn.” 1968. Doctoral Dissertation, Montana State University. Accessed March 08, 2021.
https://scholarworks.montana.edu/xmlui/handle/1/4186.
MLA Handbook (7th Edition):
Ericson, John Edward. “Serology as a technique to detect major genetic differences in the genus Agropyron Gaertn.” 1968. Web. 08 Mar 2021.
Vancouver:
Ericson JE. Serology as a technique to detect major genetic differences in the genus Agropyron Gaertn. [Internet] [Doctoral dissertation]. Montana State University; 1968. [cited 2021 Mar 08].
Available from: https://scholarworks.montana.edu/xmlui/handle/1/4186.
Council of Science Editors:
Ericson JE. Serology as a technique to detect major genetic differences in the genus Agropyron Gaertn. [Doctoral Dissertation]. Montana State University; 1968. Available from: https://scholarworks.montana.edu/xmlui/handle/1/4186
University of Arizona
6.
Tom, Baldwin H.
ATTEMPTS TO INTERFERE WITH THE SENSITIZATION AND SKIN-TEST RESPONSE OF DELAYED HYPERSENSITIVITY TO SIMPLE CHEMICALS IN THE GUINEA PIG
.
Degree: 1970, University of Arizona
URL: http://hdl.handle.net/10150/287603
Subjects/Keywords: Serology.;
Skin – Inflammation.
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APA (6th Edition):
Tom, B. H. (1970). ATTEMPTS TO INTERFERE WITH THE SENSITIZATION AND SKIN-TEST RESPONSE OF DELAYED HYPERSENSITIVITY TO SIMPLE CHEMICALS IN THE GUINEA PIG
. (Doctoral Dissertation). University of Arizona. Retrieved from http://hdl.handle.net/10150/287603
Chicago Manual of Style (16th Edition):
Tom, Baldwin H. “ATTEMPTS TO INTERFERE WITH THE SENSITIZATION AND SKIN-TEST RESPONSE OF DELAYED HYPERSENSITIVITY TO SIMPLE CHEMICALS IN THE GUINEA PIG
.” 1970. Doctoral Dissertation, University of Arizona. Accessed March 08, 2021.
http://hdl.handle.net/10150/287603.
MLA Handbook (7th Edition):
Tom, Baldwin H. “ATTEMPTS TO INTERFERE WITH THE SENSITIZATION AND SKIN-TEST RESPONSE OF DELAYED HYPERSENSITIVITY TO SIMPLE CHEMICALS IN THE GUINEA PIG
.” 1970. Web. 08 Mar 2021.
Vancouver:
Tom BH. ATTEMPTS TO INTERFERE WITH THE SENSITIZATION AND SKIN-TEST RESPONSE OF DELAYED HYPERSENSITIVITY TO SIMPLE CHEMICALS IN THE GUINEA PIG
. [Internet] [Doctoral dissertation]. University of Arizona; 1970. [cited 2021 Mar 08].
Available from: http://hdl.handle.net/10150/287603.
Council of Science Editors:
Tom BH. ATTEMPTS TO INTERFERE WITH THE SENSITIZATION AND SKIN-TEST RESPONSE OF DELAYED HYPERSENSITIVITY TO SIMPLE CHEMICALS IN THE GUINEA PIG
. [Doctoral Dissertation]. University of Arizona; 1970. Available from: http://hdl.handle.net/10150/287603
Kansas State University
7.
Ghalib, Hashim Warsama.
Ecology and serology of the Aeromonads and their relationship to Brucella abortus.
Degree: 1977, Kansas State University
URL: http://hdl.handle.net/2097/11553
Subjects/Keywords: Aeromonads; Brucellosis; Serology
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APA (6th Edition):
Ghalib, H. W. (1977). Ecology and serology of the Aeromonads and their relationship to Brucella abortus. (Thesis). Kansas State University. Retrieved from http://hdl.handle.net/2097/11553
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Ghalib, Hashim Warsama. “Ecology and serology of the Aeromonads and their relationship to Brucella abortus.” 1977. Thesis, Kansas State University. Accessed March 08, 2021.
http://hdl.handle.net/2097/11553.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Ghalib, Hashim Warsama. “Ecology and serology of the Aeromonads and their relationship to Brucella abortus.” 1977. Web. 08 Mar 2021.
Vancouver:
Ghalib HW. Ecology and serology of the Aeromonads and their relationship to Brucella abortus. [Internet] [Thesis]. Kansas State University; 1977. [cited 2021 Mar 08].
Available from: http://hdl.handle.net/2097/11553.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Ghalib HW. Ecology and serology of the Aeromonads and their relationship to Brucella abortus. [Thesis]. Kansas State University; 1977. Available from: http://hdl.handle.net/2097/11553
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Sydney
8.
Brennan, Anthea.
Toxoplasma gondii infection in Australian felines
.
Degree: 2015, University of Sydney
URL: http://hdl.handle.net/2123/15060
► Toxoplasma gondii is a significant human and animal parasite with worldwide occurrence. The population structure and prevalence in definitive hosts and intermediate hosts has been…
(more)
▼ Toxoplasma gondii is a significant human and animal parasite with worldwide occurrence. The population structure and prevalence in definitive hosts and intermediate hosts has been well studied in a number of regions including North and South America and Europe. While Toxoplasma gondii has been previously reported in a number of mammalian species in Australia, there is a surprising lack of information regarding the population structure of T.gondii in Australia and current rates of exposure in domestic companion animal hosts. Members of the felidae family are the only known definitive host of T. gondii in which sexual reproduction of the parasite occurs.Sexual reproduction results in shedding of environmentally resillient oocysts in faeces. All strains of T. gondii can be traced back to a feline host. As such, an understanding of T. gondii infection among feline hosts is an important first step to understanding general prevalence and population structure of the parasite in a particular area. The seroprevalence of T. gondii in cats worldwide is estimated to be 30-40%. In addition to being a definitive host of T. gondii, domestic cats are susceptible to clinical disease due to T.gondii. Although uncommon, feline toxoplasmosis occurs in immunosuppressed cats, congenitally infected kittens and occasionally in otherwise clinically healthy individuals. Little is known about whether parasite genotype is associated with severity of disease in naturally occurring toxoplasmosis of domestic cats. The first aim of this research was to expand knowledge of feline T. gondii infections in Australia by determining the seroprevalence of T. gondii in owned Australian cats and identifying riskfactors for infection. The second aim was to determine whether the genotype of T. gondii is a significant determinant of whether cats develop clinical toxoplasmosis. To estimate seroprevalence of T. gondii in Australian owned cats, Toxoplasma specific IgG ELISAs were performed on sera from 425 owned domestic Australian cats. A multivariate 12 analysis of the results from a questionnaire given to the owners was used to evaluate lifestyle factors which could contribute to increased likelihood of infection. Of the 425 cats tested in this study 38% (n=162) were seropositive. The prevalence in different geographic regions ranged from 16-71%. Cats fed raw beef or raw kangaroo in their diet or cats that hunted rodents were significantly more likely to be seropositive To identify the genotypes associated with latent and active infections in Australian cats, tissue samples were collected from cats undergoing routine post mortem examination at the University Veterinary Teaching Hospital (n=28). Serology to detect T. gondii specific IgG was performed after collection of heart blood from cats of unknown T. gondii serostatus. Cases were chosen based on the likelihood of a cat being exposed to the parasite. Results of a PCR targeting the B1 gene to detect T. gondii DNA were positive in tissue samples from 11 of 17 (65%) seropositive cats tested including four…
Subjects/Keywords: Toxoplasma gondii;
protozoa;
Australia;
feline;
genotype;
serology
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Manager
APA (6th Edition):
Brennan, A. (2015). Toxoplasma gondii infection in Australian felines
. (Thesis). University of Sydney. Retrieved from http://hdl.handle.net/2123/15060
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Brennan, Anthea. “Toxoplasma gondii infection in Australian felines
.” 2015. Thesis, University of Sydney. Accessed March 08, 2021.
http://hdl.handle.net/2123/15060.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Brennan, Anthea. “Toxoplasma gondii infection in Australian felines
.” 2015. Web. 08 Mar 2021.
Vancouver:
Brennan A. Toxoplasma gondii infection in Australian felines
. [Internet] [Thesis]. University of Sydney; 2015. [cited 2021 Mar 08].
Available from: http://hdl.handle.net/2123/15060.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Brennan A. Toxoplasma gondii infection in Australian felines
. [Thesis]. University of Sydney; 2015. Available from: http://hdl.handle.net/2123/15060
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Princeton University
9.
Winter, Amy Kaye.
Integrating Data, Demography, and Dynamics to Inform Vaccination Policy: Measles and Rubella in a Changing World
.
Degree: PhD, 2016, Princeton University
URL: http://arks.princeton.edu/ark:/88435/dsp01np193c662
► In this dissertation, I explore public health questions related to the current status of measles and rubella incidence and immunity, and the control of measles…
(more)
▼ In this dissertation, I explore public health questions related to the current status of measles and rubella incidence and immunity, and the control of measles and rubella infections. Great strides have been made in the reduction of measles and rubella infections via vaccination. However, measles remains a significant cause of morbidity (20 million annual cases) and mortality (115,000 annual deaths); and rubella infection, typically mild among childhood, among pregnant women can cause the birth of an infant with congenital rubella syndrome (CRS) (105,000 annual CRS cases).
I built on an existing age-structured mathematical model to evaluate important public health questions in the context of measles and rubella control efforts. Mathematical models are increasingly relied upon for vaccination program design because formally framing the core mechanisms associated with transmission dynamics is essential to capturing important and potentially devastating non-linear effects in the dynamics of these infections.
In chapter 1, I evaluated the potential of introducing rubella-containing vaccine in India to reduce the burden of CRS, and found that introduction of the rubella vaccine will likely result in cumulative decreases in CRS. However, the effect of rubella vaccine introduction on the transient annual incidence of CRS is highly sensitive to rubella’s basic reproductive number. We identified risk factors that can be used to highlight regions most at risk of transient increases in CRS burden post-rubella vaccine introduction.
In chapter 2, I explored the use of a nested serological survey within the fever-rash surveil- lance system in Madagascar to estimate measles population immunity. We found discrepancies between direct and indirect empirical estimates of population immunity by age. However, both estimates indicated that Madagascar is at risk of a large measles outbreak. We evaluated the potential of measles Supplementary Immunization Activities to reduce this risk.
In chapter 3, I analyzed the strengths and limitations of rubella IgG serological data to characterize rubella epidemiological parameters and CRS incidence. We laid out in detail the nuanced biases from analytic method and survey design, which can be used by public health officials to better interpret past serological survey estimates, and to design, implement, and interpret future serological surveys.
Advisors/Committee Members: Metcalf, C. Jessica E (advisor).
Subjects/Keywords: India;
Madagascar;
Measles;
Rubella;
Serology;
Vaccination
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APA (6th Edition):
Winter, A. K. (2016). Integrating Data, Demography, and Dynamics to Inform Vaccination Policy: Measles and Rubella in a Changing World
. (Doctoral Dissertation). Princeton University. Retrieved from http://arks.princeton.edu/ark:/88435/dsp01np193c662
Chicago Manual of Style (16th Edition):
Winter, Amy Kaye. “Integrating Data, Demography, and Dynamics to Inform Vaccination Policy: Measles and Rubella in a Changing World
.” 2016. Doctoral Dissertation, Princeton University. Accessed March 08, 2021.
http://arks.princeton.edu/ark:/88435/dsp01np193c662.
MLA Handbook (7th Edition):
Winter, Amy Kaye. “Integrating Data, Demography, and Dynamics to Inform Vaccination Policy: Measles and Rubella in a Changing World
.” 2016. Web. 08 Mar 2021.
Vancouver:
Winter AK. Integrating Data, Demography, and Dynamics to Inform Vaccination Policy: Measles and Rubella in a Changing World
. [Internet] [Doctoral dissertation]. Princeton University; 2016. [cited 2021 Mar 08].
Available from: http://arks.princeton.edu/ark:/88435/dsp01np193c662.
Council of Science Editors:
Winter AK. Integrating Data, Demography, and Dynamics to Inform Vaccination Policy: Measles and Rubella in a Changing World
. [Doctoral Dissertation]. Princeton University; 2016. Available from: http://arks.princeton.edu/ark:/88435/dsp01np193c662
Freie Universität Berlin
10.
Soilemetzidou, Eirini.
Seroprävalenz von Influenza-A-Viren bei wilden Säugetieren.
Degree: 2020, Freie Universität Berlin
URL: http://dx.doi.org/10.17169/refubium-28326
► Emerging infectious diseases, particularly zoonotic diseases, have been the focus of scientific and public interest in recent years. Influenza A viruses (IAV), currently and historically…
(more)
▼ Emerging infectious diseases, particularly zoonotic diseases, have been the focus of scientific and public interest in recent years. Influenza A viruses (IAV), currently and historically have been zoonotic agents of great importance for both human and animal health. There is evidence of infection and exposure of numerous avian and mammalian species.
Furthermore, studies have shown the potential of influenza viruses to cross species barriers, infecting many domestic and wild mammalian species. This thesis investigated exposure of multiple wild mammalian species to IAV to assess their potential role in the transmission and evolution of the viruses using serological methods. A number of factors were identified that promote influenza transmission and exposure. Contact between domestic and wildlife species, such as Asiatic wild asses with their sympatric relatives, was one factor. Furthermore, animals with both avian and mammalian influenza receptors in their respiratory tract, such as Equids, are more susceptible to influenza A virus infection, as we demonstrate in Chapter 3. The same effect is demonstrated in Chapter 2, where we showed that carnivores that consume birds had a higher diversity and greater exposure to AIV, while sociality and phylogenetic relationship does not seem to drive influenza exposure.
If their susceptibility remains a dead-end infection, or are we facing a new endemic, and therefore potentially epidemic or pandemic infection, remains an open question. Serological surveys provide information about past infections, but molecular methods are needed in order to draw conclusions on the evolution and adaptation of the viruses in these potential new hosts. Integration of lo pathogenic IAV, LPAIV, in epidemiological studies, as well as IAV that are thought to no longer circulate, like H7N7 in equids, is of great importance. Wildlife may represent an unrecognized ecological niche for IAV. Additionally, constant and rapid change in the environment, such as climate change, agriculture practices, and habitat destruction, are influencing hosts, pathogens, and diseases and must be taken in account in wildlife disease surveillance studies. Integration and analysis of data from all these different sources, including animal and human data, will give us the tools to perform risk analysis, and apply possible control or prevention schemes.
Advisors/Committee Members: female (gender), Greenwood, Alex (firstReferee), Osterrieder, Klaus (furtherReferee), Doherr, Marcus (furtherReferee).
Subjects/Keywords: influenza; wild mammals; serology; virus; ddc:500
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MLA ·
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CSE |
Export
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APA (6th Edition):
Soilemetzidou, E. (2020). Seroprävalenz von Influenza-A-Viren bei wilden Säugetieren. (Thesis). Freie Universität Berlin. Retrieved from http://dx.doi.org/10.17169/refubium-28326
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Soilemetzidou, Eirini. “Seroprävalenz von Influenza-A-Viren bei wilden Säugetieren.” 2020. Thesis, Freie Universität Berlin. Accessed March 08, 2021.
http://dx.doi.org/10.17169/refubium-28326.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Soilemetzidou, Eirini. “Seroprävalenz von Influenza-A-Viren bei wilden Säugetieren.” 2020. Web. 08 Mar 2021.
Vancouver:
Soilemetzidou E. Seroprävalenz von Influenza-A-Viren bei wilden Säugetieren. [Internet] [Thesis]. Freie Universität Berlin; 2020. [cited 2021 Mar 08].
Available from: http://dx.doi.org/10.17169/refubium-28326.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Soilemetzidou E. Seroprävalenz von Influenza-A-Viren bei wilden Säugetieren. [Thesis]. Freie Universität Berlin; 2020. Available from: http://dx.doi.org/10.17169/refubium-28326
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Technical University of Lisbon
11.
Gomes, Catarina Adriano Vaz de Carvalho.
Fasciolose em bovinos de engorda.
Degree: 2012, Technical University of Lisbon
URL: http://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/4848
► Dissertação de Mestrado Integrado em Medicina Veterinária.
A produção animal representa um papel crucial na economia de um país e é, por isso, muito importante…
(more)
▼ Dissertação de Mestrado Integrado em Medicina Veterinária.
A produção animal representa um papel crucial na economia de um país e é, por isso, muito importante garantir a sua rentabilização. Por vezes é descurada a importância de afeções como as parasitoses que têm grande influência na saúde e bem-estar animal.
Neste estudo foi avaliada a importância do tremátode Fasciola hepatica na engorda bovina, parasita hepático com ciclo heteroxeno, participando como hospedeiro intermediário o caracol L. truncatula. Este parasita causa lesões fibróticas no parênquima hepático levando à perda da sua função, diminuindo os níveis de produção e perdas diretas por reprovação do órgão em matadouro. Foi ainda objetivo deste estudo avaliar a importância da desparasitação contra esta parasitose num tipo de produção semi-intensivo e comparar a eficácia de dois princípios ativos – Closantel e Clorsulon.
Serviu de base para este estudo uma exploração de bovinos em regime semi-intensivo, localizada na região da Moita do Ribatejo, Portugal, considerada endémica de F. hepatica. Foram incluídos no estudo 19 bovinos machos com idades compreendidas entre os 9 e 25 meses aos quais se aplicou um único tratamento com os dois fármacos a ser testados. Foi recolhida uma amostra de fezes no dia da aplicação e duas amostras após tratamento com quinze dias de intervalo. Realizaram-se análises coprológicas: técnica de sedimentação simples e de McMaster Modificado; e análise serológica por ELISA indireto com uso da proteína recombinante APS. Foram inspecionados os dezanove fígados no momento do abate e uma amostra destes (n=5) analisada para deteção de fascíolas no seu parênquima.
Todas as amostras processadas com a técnica de sedimentação simples apresentaram resultados negativos. Por sua vez, com a técnica McMaster Modificado obtiveram-se 73,68% de resultados positivos na primeira amostra e uma diminuição para 31,58% na última amostra, obtendo-se uma Redução de Contagem de Ovos Fecais entre 90 e 95%. Na análise serológica obtiveram-se três resultados positivos. Quanto à inspeção dos fígados, 78,94% destes foram reprovados devido a fasciolose.
Independentemente da eficácia do tratamento, o tempo que decorre desde a sua aplicação até ao abate não é suficiente para eliminar as lesões que são causa de reprovação a nível de matadouro. Assim, conclui-se que o mais importante é prevenir a infeção e não tratá-la, devendo por isso investir-se no controlo do ciclo do parasita com um bom maneio das pastagens e dos animais e utilizar a desparasitação apenas como auxílio na profilaxia.
ABSTRACT - Fasciolosis in beef cattle -
against this parasite in a semi-intensive production and compare the effectiveness of two drugs – closantel and clorsulon.
This study was done in an semi-intensive cattle fattening farm, located in Moita do Ribatejo, Portugal. This is an endemic area of F. hepatica. Nineteen bulls, aged between 9 and 25 months, were included in this study, to which was applied a single treatment with the two drugs being tested. Was taken a sample of faeces…
Advisors/Committee Members: Guerreiro, Dário Alexandre Nunes de Sá, Stilwell, George Thomas.
Subjects/Keywords: Bovinos; F. hepatica; APS; Diagnóstico; Coprologia; Serologia; Cattle; Diagnosis; Coprology; Serology
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APA (6th Edition):
Gomes, C. A. V. d. C. (2012). Fasciolose em bovinos de engorda. (Thesis). Technical University of Lisbon. Retrieved from http://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/4848
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Gomes, Catarina Adriano Vaz de Carvalho. “Fasciolose em bovinos de engorda.” 2012. Thesis, Technical University of Lisbon. Accessed March 08, 2021.
http://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/4848.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Gomes, Catarina Adriano Vaz de Carvalho. “Fasciolose em bovinos de engorda.” 2012. Web. 08 Mar 2021.
Vancouver:
Gomes CAVdC. Fasciolose em bovinos de engorda. [Internet] [Thesis]. Technical University of Lisbon; 2012. [cited 2021 Mar 08].
Available from: http://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/4848.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Gomes CAVdC. Fasciolose em bovinos de engorda. [Thesis]. Technical University of Lisbon; 2012. Available from: http://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/4848
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
12.
AracÃlia Gurgel Rodrigues.
Estudo do polimorfismo C2029T no gene do receptor toll-like tipo 2 e da resposta imune humoral em pacientes com hansenÃase.
Degree: Master, 2008, Universidade Federal do Ceará
URL: http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=3703
;
► Apesar do empenho do MinistÃrio da SaÃde para a eliminaÃÃo da hansenÃase, o Brasil à o segundo paÃs em nÃmero de casos no mundo, precedido…
(more)
▼ Apesar do empenho do MinistÃrio da SaÃde para a eliminaÃÃo da hansenÃase, o Brasil à o segundo paÃs em nÃmero de casos no mundo, precedido pela Ãndia, e responsÃvel por 80% dos casos no continente americano, sendo o Nordeste do paÃs e, em especial, o Estado do Cearà considerado uma regiÃo de alta endemicidade. De acordo com a classificaÃÃo de Ridley e Jopling, as formas clÃnicas dividem-se em virchowiana, dimorfa-virchowiana, dimorfa-dimorfa, dimorfa-tuberculÃide e tuberculÃide. O trabalho foi realizado com 87 pacientes com hansenÃase, sendo 51,72% do sexo feminino e 48,3% do sexo masculino; destes 87 pacientes, 77,01% vacinados em algum perÃodo da vida entre a infÃncia a adolescÃncia. Os pacientes incluÃdos no estudo encontravam-se nÃo tratados (n=23) ou em tratamento (n=64), apresentando a maioria dos pacientes a hansenÃase pela primeira vez e alguns apresentavam recidiva (n=11). A sorologia de IgG sÃrica anti-PGL1 foi realizada em 83 pacientes, tendo sido as concentraÃÃes de maiores diferenÃas ocorridas entre os grupos com a forma tuberculÃide e dimorfa-tuberculÃide e o grupo com a forma dimorfa-virchowiana. As 87 amostras analisadas foram amplificadas quanto à seqÃÃncia de 171 pb de uma regiÃo altamente conservada dos aminoÃcidos 671-692 do C-terminal no domÃnio intracelular do receptor Toll-like 2 e foi aplicada a tÃcnica de AnÃlise do Polimorfismo Conformacional de Fita Ãnica (SSCP). O perfil eletroforÃtico das amostras testadas encontradas foram de duas e trÃs bandas, mostrando-se necessÃrio o sequenciamento,este apresentou heterozigose marcado pela presenÃa das bases C e T na posiÃÃo C2029T, e alÃm de dois outros perfis de heterozigose nas posiÃÃes C2006T (encontrado em todas as amostras sequenciadas) e T2008G (amostra 82). O trabalho sugere que houve heterozigose na regiÃo do Ãxon 3 do gene do receptor toll-like tipo 2, diferentemente do encontrado por Kang e Chae (2001) o que pode significar que o perfil de suscetibilidade em nossa populaÃÃo à distinta daqueles encontrados na Ãndia e na CorÃia.
Although several efforts from Ministry of Health have been made in order to eliminate leprosy, Brazil is still the second country with the highest number of cases in world after India and is responsible for 80% of the cases in the American continent, the Ceara state situated in the Norheastern region is considered to have high incidence rates of leprosy cases. According to Ridley and Jopling classification, the clinical forms of leprosy can be divided in lepromatous leprosy, borderline lepromatous leprosy, borderline borderline leprosy, borderline tuberculoid leprosy, and tuberculoid leprosy. This work was done with 87 patients with leprosy, being 51.72 % of the female gender and 48.3% of the male gender; from the total of patients, 77.0% had been vaccinated with BCG once in life. All the patients enrolled in the study were not treated (n=23) or in treatment (n=64). Most of the patients had suffered from leprosy for the first time and some (n=11) had suffered from leprosy recidive. The anti-PGL1 serum IgG…
Advisors/Committee Members: Aparecida Tiemi NagÃo Dias, Lilia Maria Carneiro CÃmara, Silvia Helena Barem Rabenhorst.
Subjects/Keywords: MICROBIOLOGIA MEDICA; HansenÃase; Serologia; Polimorfismo GenÃtico; Leprosy, Serology, Genetic Polymorphism
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APA ·
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Export
to Zotero / EndNote / Reference
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APA (6th Edition):
Rodrigues, A. G. (2008). Estudo do polimorfismo C2029T no gene do receptor toll-like tipo 2 e da resposta imune humoral em pacientes com hansenÃase. (Masters Thesis). Universidade Federal do Ceará. Retrieved from http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=3703 ;
Chicago Manual of Style (16th Edition):
Rodrigues, AracÃlia Gurgel. “Estudo do polimorfismo C2029T no gene do receptor toll-like tipo 2 e da resposta imune humoral em pacientes com hansenÃase.” 2008. Masters Thesis, Universidade Federal do Ceará. Accessed March 08, 2021.
http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=3703 ;.
MLA Handbook (7th Edition):
Rodrigues, AracÃlia Gurgel. “Estudo do polimorfismo C2029T no gene do receptor toll-like tipo 2 e da resposta imune humoral em pacientes com hansenÃase.” 2008. Web. 08 Mar 2021.
Vancouver:
Rodrigues AG. Estudo do polimorfismo C2029T no gene do receptor toll-like tipo 2 e da resposta imune humoral em pacientes com hansenÃase. [Internet] [Masters thesis]. Universidade Federal do Ceará 2008. [cited 2021 Mar 08].
Available from: http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=3703 ;.
Council of Science Editors:
Rodrigues AG. Estudo do polimorfismo C2029T no gene do receptor toll-like tipo 2 e da resposta imune humoral em pacientes com hansenÃase. [Masters Thesis]. Universidade Federal do Ceará 2008. Available from: http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=3703 ;
13.
Rafael Lima de Oliveira.
Pesquisa de anticorpos IgG anti-Leishmania infantum em raposas (Cerdocyon thous) de vida livre e de cativeiro e em cães domésticos (Canis familiaris) em Unidades de Conservação do Estado de Pernambuco.
Degree: 2012, Universidade Federal Rural de Pernambuco
URL: http://200.17.137.108/tde_busca/arquivo.php?codArquivo=1606
► Visceral Leishmaniasis (VL) is a zoonosis transmitted by sand flies caused by several Leishmania species and that can infect humans and domestic and wild animals.…
(more)
▼ Visceral Leishmaniasis (VL) is a zoonosis transmitted by sand flies caused by several Leishmania species and that can infect humans and domestic and wild animals. In Brazil, the growth in the number of cases of Canine Visceral Leishmaniasis (CVL) is associated with the adaptation of its vector and dispersion of Leishmania infantum among domestic and wild hosts in urban and periurban areas. The objective of this work contribute to the epidemiological study of CVL in the state of Pernambuco by identification of the frequency IgG antibodies anti-Leishmania infantum in free-living and captives crab-eating foxes (Cerdocyon thous) and domestic dogs (Canis familiaris) from Conservation Unit of the State of Pernambuco and its surroundings. Blood samples were obtained of 107 animals, 18 crabeating foxes (four free-living and 14 captives) and of 89 dogs (85 domiciled and four strays). Bone marrow samples were obtained of nine crab-eating foxes and three dogs. The IFAT and ELISA were performed on blood samples of 107 dogs and PCR was performed on 12 samples of bone marrow. Of the 18 crab-eating foxes examined, one (5.5%) were seropositive to the ELISA and 89 dogs examined, 29.21% were positive by IFAT, 35.95% positive by ELISA and 19.10% were positive in both tests simultaneously. None of the samples was positive by PCR. It is concluded that the presence of IgG antibodies anti-L. infantum in a free-living crabeating fox and in a stray dog in the wild environment suggested the existence of a local sylvatic cycle of Leishmania infantum in one of the areas of study. The presence of seropositive dogs inside and around of these wild areas indicated that animals can act as dispersers of the infectious agent to humans and to the non-infected dogs. This is the first occurrence of IgG antibodies to Leishmania infantum in free-living crab-eating fox and dogs trapped inside of the Conservation Units of the State of Pernambuco, Brazil.
A Leishmaniose Visceral (LV) é uma zoonose transmitida por flebotomíneos, sendo causada por diversas espécies de Leishmania e que podem infectar seres humanos, e animais domésticos e selvagens. No Brasil, o crescimento no número de casos de Leishmaniose Visceral Canina (LVC) é associado com a adaptação de seu vetor e a dispersão da Leishmania infantum entre os hospedeiros domésticos e silvestres no ambiente urbano e periurbano. Objetivou-se com este trabalho contribuir com o estudo epidemiológico da LVC no Estado de Pernambuco, mediante a identificação da frequência anticorpos IgG anti-Leishmania infantum em raposas (Cerdocyon thous) de vida livre e de cativeiro e em cães domésticos (Canis familiaris) oriundos de Unidades de Conservação do Estado de Pernambuco e no seu entorno. Ao todo, foram obtidas amostras de sangue de 107 animais, sendo 18 raposas (quatro de vida livre e 14 de cativeiro) e de 89 cães (85 domiciliados e quatro errantes). Em nove raposas e três cães também foram obtidas amostras de medula óssea. Os testes de RIFI e ELISA foram realizados nas 107 amostras de sangue dos canídeos e a PCR…
Advisors/Committee Members: Rinaldo Aparecido Mota, Jean Carlos Ramos da Silva.
Subjects/Keywords: Calazar; Canídeos; Diagnóstico; Sorologia; MEDICINA VETERINARIA; Kala-azar; Canids; Diagnostic; Serology
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APA ·
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APA (6th Edition):
Oliveira, R. L. d. (2012). Pesquisa de anticorpos IgG anti-Leishmania infantum em raposas (Cerdocyon thous) de vida livre e de cativeiro e em cães domésticos (Canis familiaris) em Unidades de Conservação do Estado de Pernambuco. (Thesis). Universidade Federal Rural de Pernambuco. Retrieved from http://200.17.137.108/tde_busca/arquivo.php?codArquivo=1606
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Oliveira, Rafael Lima de. “Pesquisa de anticorpos IgG anti-Leishmania infantum em raposas (Cerdocyon thous) de vida livre e de cativeiro e em cães domésticos (Canis familiaris) em Unidades de Conservação do Estado de Pernambuco.” 2012. Thesis, Universidade Federal Rural de Pernambuco. Accessed March 08, 2021.
http://200.17.137.108/tde_busca/arquivo.php?codArquivo=1606.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Oliveira, Rafael Lima de. “Pesquisa de anticorpos IgG anti-Leishmania infantum em raposas (Cerdocyon thous) de vida livre e de cativeiro e em cães domésticos (Canis familiaris) em Unidades de Conservação do Estado de Pernambuco.” 2012. Web. 08 Mar 2021.
Vancouver:
Oliveira RLd. Pesquisa de anticorpos IgG anti-Leishmania infantum em raposas (Cerdocyon thous) de vida livre e de cativeiro e em cães domésticos (Canis familiaris) em Unidades de Conservação do Estado de Pernambuco. [Internet] [Thesis]. Universidade Federal Rural de Pernambuco; 2012. [cited 2021 Mar 08].
Available from: http://200.17.137.108/tde_busca/arquivo.php?codArquivo=1606.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Oliveira RLd. Pesquisa de anticorpos IgG anti-Leishmania infantum em raposas (Cerdocyon thous) de vida livre e de cativeiro e em cães domésticos (Canis familiaris) em Unidades de Conservação do Estado de Pernambuco. [Thesis]. Universidade Federal Rural de Pernambuco; 2012. Available from: http://200.17.137.108/tde_busca/arquivo.php?codArquivo=1606
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
14.
Rafael Lima de Oliveira.
Pesquisa de anticorpos IgG anti-Leishmania infantum em raposas (Cerdocyon thous) de vida livre e de cativeiro e em cães domésticos (Canis familiaris) em Unidades de Conservação do Estado de Pernambuco.
Degree: 2012, Universidade Federal Rural de Pernambuco
URL: http://200.17.137.108/tde_busca/arquivo.php?codArquivo=1606
► A Leishmaniose Visceral (LV) é uma zoonose transmitida por flebotomíneos, sendo causada por diversas espécies de Leishmania e que podem infectar seres humanos, e animais…
(more)
▼ A Leishmaniose Visceral (LV) é uma zoonose transmitida por flebotomíneos, sendo causada por diversas espécies de Leishmania e que podem infectar seres humanos, e animais domésticos e selvagens. No Brasil, o crescimento no número de casos de Leishmaniose Visceral Canina (LVC) é associado com a adaptação de seu vetor e a dispersão da Leishmania infantum entre os hospedeiros domésticos e silvestres no ambiente urbano e periurbano. Objetivou-se com este trabalho contribuir com o estudo epidemiológico da LVC no Estado de Pernambuco, mediante a identificação da frequência anticorpos IgG anti-Leishmania infantum em raposas (Cerdocyon thous) de vida livre e de cativeiro e em cães domésticos (Canis familiaris) oriundos de Unidades de Conservação do Estado de Pernambuco e no seu entorno. Ao todo, foram obtidas amostras de sangue de 107 animais, sendo 18 raposas (quatro de vida livre e 14 de cativeiro) e de 89 cães (85 domiciliados e quatro errantes). Em nove raposas e três cães também foram obtidas amostras de medula óssea. Os testes de RIFI e ELISA foram realizados nas 107 amostras de sangue dos canídeos e a PCR foi realizada nas 12 amostras de medula óssea. Das 18 raposas examinadas, uma (5,5%) foi soropositiva ao ELISA e dos 89 cães examinados, 29,21% foram soropositivos pela RIFI, 35,95% pelo ELISA e 19,10% foram soropositivos em ambos os testes simultaneamente. Nenhuma das amostras foi positiva na PCR. Concluiu-se que a ocorrência de anticorpos IgG anti-L. infantum em uma raposa de vida livre e um cão errante no mesmo ambiente silvestre sugeriu a existência de um ciclo silvático da Leishmania infantum em uma das áreas de estudo. A presença de cães soropositivos no interior e no entorno de áreas silvestres indicou que estes animais podem atuar como dispersores do agente infeccioso para populações humanas e para cães não infectados. Esta é a primeira ocorrência de anticorpos IgG anti-Leishmania infantum em raposa de vida livre e em cães capturados no interior de Unidades Conservação do Estado de Pernambuco, Brasil.
Visceral Leishmaniasis (VL) is a zoonosis transmitted by sand flies caused by several Leishmania species and that can infect humans and domestic and wild animals. In Brazil, the growth in the number of cases of Canine Visceral Leishmaniasis (CVL) is associated with the adaptation of its vector and dispersion of Leishmania infantum among domestic and wild hosts in urban and periurban areas. The objective of this work contribute to the epidemiological study of CVL in the state of Pernambuco by identification of the frequency IgG antibodies anti-Leishmania infantum in free-living and captives crab-eating foxes (Cerdocyon thous) and domestic dogs (Canis familiaris) from Conservation Unit of the State of Pernambuco and its surroundings. Blood samples were obtained of 107 animals, 18 crabeating foxes (four free-living and 14 captives) and of 89 dogs (85 domiciled and four strays). Bone marrow samples were obtained of nine crab-eating foxes and three dogs. The IFAT and ELISA were performed on blood samples of 107…
Advisors/Committee Members: Rinaldo Aparecido Mota, Jean Carlos Ramos da Silva.
Subjects/Keywords: Calazar; Canídeos; Diagnóstico; Sorologia; MEDICINA VETERINARIA; Kala-azar; Canids; Diagnostic; Serology
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APA (6th Edition):
Oliveira, R. L. d. (2012). Pesquisa de anticorpos IgG anti-Leishmania infantum em raposas (Cerdocyon thous) de vida livre e de cativeiro e em cães domésticos (Canis familiaris) em Unidades de Conservação do Estado de Pernambuco. (Thesis). Universidade Federal Rural de Pernambuco. Retrieved from http://200.17.137.108/tde_busca/arquivo.php?codArquivo=1606
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Oliveira, Rafael Lima de. “Pesquisa de anticorpos IgG anti-Leishmania infantum em raposas (Cerdocyon thous) de vida livre e de cativeiro e em cães domésticos (Canis familiaris) em Unidades de Conservação do Estado de Pernambuco.” 2012. Thesis, Universidade Federal Rural de Pernambuco. Accessed March 08, 2021.
http://200.17.137.108/tde_busca/arquivo.php?codArquivo=1606.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Oliveira, Rafael Lima de. “Pesquisa de anticorpos IgG anti-Leishmania infantum em raposas (Cerdocyon thous) de vida livre e de cativeiro e em cães domésticos (Canis familiaris) em Unidades de Conservação do Estado de Pernambuco.” 2012. Web. 08 Mar 2021.
Vancouver:
Oliveira RLd. Pesquisa de anticorpos IgG anti-Leishmania infantum em raposas (Cerdocyon thous) de vida livre e de cativeiro e em cães domésticos (Canis familiaris) em Unidades de Conservação do Estado de Pernambuco. [Internet] [Thesis]. Universidade Federal Rural de Pernambuco; 2012. [cited 2021 Mar 08].
Available from: http://200.17.137.108/tde_busca/arquivo.php?codArquivo=1606.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Oliveira RLd. Pesquisa de anticorpos IgG anti-Leishmania infantum em raposas (Cerdocyon thous) de vida livre e de cativeiro e em cães domésticos (Canis familiaris) em Unidades de Conservação do Estado de Pernambuco. [Thesis]. Universidade Federal Rural de Pernambuco; 2012. Available from: http://200.17.137.108/tde_busca/arquivo.php?codArquivo=1606
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
15.
Plautino de Oliveira Laroque.
Pesquisa soro-epidemiológica para arbovírus em macaco-prego-galego (Cebus flavius) de vida livre no estado da Paraíba e em macaco-prego (Cebus libidinosus) de cativeiro no nordeste do Brasil.
Degree: 2013, Universidade Federal Rural de Pernambuco
URL: http://200.17.137.108/tde_busca/arquivo.php?codArquivo=1982
► Este estudo descreveu a primeira investigação de anticorpos para arbovírus em primatas não humanos do Novo Mundo em cinco estados do Nordeste Brasileiro. No período…
(more)
▼ Este estudo descreveu a primeira investigação de anticorpos para arbovírus em primatas não humanos do Novo Mundo em cinco estados do Nordeste Brasileiro. No período de março de 2008 a setembro de 2010 foram colhidos soros sanguíneos de 31 macacos-prego-galego (Cebus flavius) de vida livre da Paraíba e 100 macacos-prego (Cebus libidinosus) de Centros de Triagem de Animais Silvestres (CETAS) dos Estados de Alagoas, Paraíba, Pernambuco, Piauí e Rio Grande do Norte. Utilizou-se o teste de inibição da hemaglutinação (IH), usando quatro unidades hemaglutinantes de 19 diferentes antígenos de arbovírus e soro diluídos a partir de 1:20. Os antígenos foram preparados pelo método de extração por sucrose-acetona e os soros dos macacos foram tratados por acetona e adsorvidos com glóbulos de ganso. Dentre os soros sanguíneos examinados, todos os macacos-prego-galego foram negativos e 46/100 (46%) de macacos-prego apresentaram anticorpos anti-arbovírus. As soropositividades por arbovírus foram: 29% para MAYV, 28% para OROV, 26% para ILHV, 19% para ESLV, 15% para EEEV, 12% para ROCV, 9% para WEEV, 9% para MUCV e 2% para YFV. Destas amostras, 15 apresentaram reação monotípica para ILHV (n=4), MAYV (n=6), SLEV (n=1), ROCV (n=2), OROV (n=1) e MUCV (n=1), sem ter havido o isolamento de agentes virais. Estes resultados sugeriram que existe intensa circulação de arbovírus na população estudada de macacos-prego em CETAS.
This study describes the first investigation of antibodies for arboviruses on non-human primates from the New World in five states of the northeast of Brazil. From March 2008 to September 2010 blood serum samples were collected of 31 free living galician capuchin monkeys (Cebus flavius) from Paraíba and of a 100 capuchin monkeys (Cebus libidinosus) of the Sorting Center for Wild Animals (CETAS) from the states of Alagoas, Paraíba, Pernambuco, Piauí and Rio Grande do Norte. The Haemagglutination-Inhibition Test (HI) was utilized and four hemagglutinating units of 19 arboviruses antigen and serum, diluted from 1:20, were used. The antigens were prepared by the sucrose-acetone extraction method and the monkeys serum were treated with acetone and adsorbed with goose blood cells. Among the blood sera examined, all galician capuchin monkeys were negative and 46/100 (46%) of capuchin monkeys presented anti-arbovirus anticorpses. The arbovirus seropositivity were 29% for MAYV, 28% for OROV, 26% for ILHV, 19% for SLV, 15% for EEEV, 12% for ROCV, 9% for WEEV, 9% for MUCV and 2% for YFV . From these samples, 15 presented monotypic reaction to ILHV (n=4), MAYV (n=6), SLEV (n=1), ROCV (n=2), OROV (n=1) e MUCV (n=1), without the isolation from the viral agents. These results suggested that there were an intense circulation of arboviruses in the studied population of capuchin monkeys in CETAS.
Advisors/Committee Members: Roberto Soares de Castro, Rita de Cássia Carvalho Maia, Jean Carlos Ramos da Silva.
Subjects/Keywords: Arboviroses; Sorologia; MEDICINA VETERINARIA; Arboviruses; Primatas; Primates; Serology
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APA (6th Edition):
Laroque, P. d. O. (2013). Pesquisa soro-epidemiológica para arbovírus em macaco-prego-galego (Cebus flavius) de vida livre no estado da Paraíba e em macaco-prego (Cebus libidinosus) de cativeiro no nordeste do Brasil. (Thesis). Universidade Federal Rural de Pernambuco. Retrieved from http://200.17.137.108/tde_busca/arquivo.php?codArquivo=1982
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Laroque, Plautino de Oliveira. “Pesquisa soro-epidemiológica para arbovírus em macaco-prego-galego (Cebus flavius) de vida livre no estado da Paraíba e em macaco-prego (Cebus libidinosus) de cativeiro no nordeste do Brasil.” 2013. Thesis, Universidade Federal Rural de Pernambuco. Accessed March 08, 2021.
http://200.17.137.108/tde_busca/arquivo.php?codArquivo=1982.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Laroque, Plautino de Oliveira. “Pesquisa soro-epidemiológica para arbovírus em macaco-prego-galego (Cebus flavius) de vida livre no estado da Paraíba e em macaco-prego (Cebus libidinosus) de cativeiro no nordeste do Brasil.” 2013. Web. 08 Mar 2021.
Vancouver:
Laroque PdO. Pesquisa soro-epidemiológica para arbovírus em macaco-prego-galego (Cebus flavius) de vida livre no estado da Paraíba e em macaco-prego (Cebus libidinosus) de cativeiro no nordeste do Brasil. [Internet] [Thesis]. Universidade Federal Rural de Pernambuco; 2013. [cited 2021 Mar 08].
Available from: http://200.17.137.108/tde_busca/arquivo.php?codArquivo=1982.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Laroque PdO. Pesquisa soro-epidemiológica para arbovírus em macaco-prego-galego (Cebus flavius) de vida livre no estado da Paraíba e em macaco-prego (Cebus libidinosus) de cativeiro no nordeste do Brasil. [Thesis]. Universidade Federal Rural de Pernambuco; 2013. Available from: http://200.17.137.108/tde_busca/arquivo.php?codArquivo=1982
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of California – Berkeley
16.
Helb, Danica Ann.
Anti-malarial Antibody Responses & Applications for Assessing Malaria Exposure.
Degree: Infectious Diseases & Immunity, 2015, University of California – Berkeley
URL: http://www.escholarship.org/uc/item/28s6v7j7
► This dissertation describes the discovery of highly informative serologic biomarkers of recent exposure to Plasmodium falciparum, the most deadly species causing malaria. An innovative approach…
(more)
▼ This dissertation describes the discovery of highly informative serologic biomarkers of recent exposure to Plasmodium falciparum, the most deadly species causing malaria. An innovative approach that combined detailed individual-level exposure data, high-throughput screening of hundreds of antibody responses, and robust statistical methods was used to identify the most informative signatures of exposure. The novel antigens described here and, more importantly, the outlined approach for biomarker discovery will allow for the development of public health and research tools that are imperative for the control and elimination of malaria. Additionally, the methodologies outlined here are highly applicable to the discovery of biomarkers of exposure for other infectious diseases. Serology has been used for decades to measure exposure to Plasmodium species and other infectious diseases. While useful, most existing assays for measuring P. falciparum exposure have been based on population-level responses to a few target antigens chosen by convenience rather than utility, resulting in relatively coarse exposure estimates. Here, detailed assessments of malaria exposure in Malian and Ugandan children were used to identify novel serologic biomarkers of malaria exposure and calibrate responses to quantitative estimates of individual exposure. The power of obtaining these individual-level estimates was illustrated by their ability to accurately identify individuals with infection in the recent past; to obtain precise estimates of malaria incidence in a population from cross-sectional samples of as few as 20 individuals; and to accurately estimate heterogeneity in recent exposure within a community using data from a single time point. Interest in the development of improved serologic assays has increased in recent years as more investment is made in malaria control and elimination. There is need for widely available, accurate estimates of malaria exposure that will allow for targeting and evaluation of public health interventions. This dissertation initiates a response to the call to develop accurate field-based assays for rapid and cost-effective assessment of malaria exposure, with the ultimate goal of putting cohort-quality data into the hands of malaria control programs.
Subjects/Keywords: Immunology; Epidemiology; Parasitology; antigen discovery; biomarkers; immunoepidemiology; malaria; Plasmodium falciparum; serology
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APA ·
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APA (6th Edition):
Helb, D. A. (2015). Anti-malarial Antibody Responses & Applications for Assessing Malaria Exposure. (Thesis). University of California – Berkeley. Retrieved from http://www.escholarship.org/uc/item/28s6v7j7
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Helb, Danica Ann. “Anti-malarial Antibody Responses & Applications for Assessing Malaria Exposure.” 2015. Thesis, University of California – Berkeley. Accessed March 08, 2021.
http://www.escholarship.org/uc/item/28s6v7j7.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Helb, Danica Ann. “Anti-malarial Antibody Responses & Applications for Assessing Malaria Exposure.” 2015. Web. 08 Mar 2021.
Vancouver:
Helb DA. Anti-malarial Antibody Responses & Applications for Assessing Malaria Exposure. [Internet] [Thesis]. University of California – Berkeley; 2015. [cited 2021 Mar 08].
Available from: http://www.escholarship.org/uc/item/28s6v7j7.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Helb DA. Anti-malarial Antibody Responses & Applications for Assessing Malaria Exposure. [Thesis]. University of California – Berkeley; 2015. Available from: http://www.escholarship.org/uc/item/28s6v7j7
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Universidade Estadual de Campinas
17.
Ribeiro, Aline Rimoldi, 1980-.
Caracterização biológica e molecular de cepas de Trypanosoma cruzi Chagas, 1909 (Kinetoplastida, Trypanosomatidae) isoladas da Bahia, Rio Grande do Sul, Santa Catarina e São Paulo: Biological and molecular characterization of strains of Trypanosoma cruzi Chagas, 1909 (Kinetoplastida, Trypanosomatidae) isolated from Bahia, Rio Grande do Sul, Santa Catarina and São Paulo.
Degree: 2014, Universidade Estadual de Campinas
URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/317469
► Abstract: Trypanosoma cruzi, a protozoan from the family Trypanosomatidae, is responsible for Chagas disease which affects about 6 to 8 million people in Latin America.…
(more)
▼ Abstract: Trypanosoma cruzi, a protozoan from the family Trypanosomatidae, is responsible for Chagas disease which affects about 6 to 8 million people in Latin America. The origin of this family can be studied through molecular techniques, for instance the investigation of the region V7V8 ¿ SSurRNA. The protozoan is subdivided in six independents groups TcI-TcVI known as Discrete Typing of Units (DTUs). The biological and molecular characterization of eleven strains of T. cruzi from the group TcI (Bolivia; Tlenti; Tmelanocephala; SC90), TcII (Famema; SC96; SI8; Y) and TcIII (QMM3; QMM5; SI5) isolated from five different species of triatomine are able to elucidate biological factors through the kinetic growth, parasitemic curve, cell infection rate, molecular characterization, metalloproteinase action, proteic and serological profile. This investigation was conducted to provide a biological and molecular characterization of T. cruzi isolated from specimens of triatomines. The group TcII of T. cruzi demonstrated higher replicative capacity in epimastigotes forms during the kinetic growth curve, followed by TcI and TcIII. The parasitemic curve demonstrated variability between Balb/c mice; however, the groups TcI, TcII and TcIII showed equivalent parasitemic profile. Furthermore, the cell infection rate in J774 cellular lineages and peritoneal macrophages was used to corroborate the biological data. The group TcI of T. cruzi demonstrated higher infection rate and less time to amastigotes forms multiplication and the peritoneal macrophages showed more attractive to T. cruzi. The molecular characterization through the V7V8 region indicated that these strains belong to DTUs TcI, TcII and TcIII. The groups segregation is important to compare the proteic profile of epimastigotes and tripomastigotes forms of T. cruzi. The groups TcI presented more proteins in acrylamide gels, which can be associated with intra-specif of TcI. The metalloproteinase action was observed in epimastigotes and tripomastigotes forms, demonstrating active and stable presence at the parasite life cycle. The serological reactivity was proven in the groups TcI, TcII and TcIII through ELISA dilutions of 1/100 to 1/12.800. Moreover, the Western Blotting technique was added to SDS-PAGE experiments in infected animals¿ serum after 50 dias and the proteic profile of Y strain was observed in nitrocellulose membrane. The results demonstrated that these eleven strains of T. cruzi have differences between the groups of the parasite. The group TcI showed higher infection rate in cells; TcII, higher value for kinetic growth and TcIII is phylogenetically closer to TcV, which is a hybrid group. The association between parasite and host is able to explain the biological and molecular differences in T. cruzi strains; for this reason, the study of eleven strains isolated from different hosts can add information to the literature and clarify some of the biological aspects of the parasite
Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS (CRUESP), Rosa, João Aristeu (advisor), Rosa, João Aristeu da (advisor), Steindel, Mário (coadvisor), Universidade Estadual de Campinas. Instituto de Biologia (institution), Programa de Pós-Graduação em Parasitologia (nameofprogram), Albuquerque, Sergio de (committee member), Azeredo-Oliveira, Maria Tercilia Vilela de (committee member), Martins, Luciamare Perinetti Alves (committee member), Pinto, Mara Cristina (committee member).
Subjects/Keywords: Trypanosoma cruzi; Caracterização; Metaloproteases; Sorologia; Trypanosoma cruzi; Characterization; Metalloproteases; Serology
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APA (6th Edition):
Ribeiro, Aline Rimoldi, 1. (2014). Caracterização biológica e molecular de cepas de Trypanosoma cruzi Chagas, 1909 (Kinetoplastida, Trypanosomatidae) isoladas da Bahia, Rio Grande do Sul, Santa Catarina e São Paulo: Biological and molecular characterization of strains of Trypanosoma cruzi Chagas, 1909 (Kinetoplastida, Trypanosomatidae) isolated from Bahia, Rio Grande do Sul, Santa Catarina and São Paulo. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/317469
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Ribeiro, Aline Rimoldi, 1980-. “Caracterização biológica e molecular de cepas de Trypanosoma cruzi Chagas, 1909 (Kinetoplastida, Trypanosomatidae) isoladas da Bahia, Rio Grande do Sul, Santa Catarina e São Paulo: Biological and molecular characterization of strains of Trypanosoma cruzi Chagas, 1909 (Kinetoplastida, Trypanosomatidae) isolated from Bahia, Rio Grande do Sul, Santa Catarina and São Paulo.” 2014. Thesis, Universidade Estadual de Campinas. Accessed March 08, 2021.
http://repositorio.unicamp.br/jspui/handle/REPOSIP/317469.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Ribeiro, Aline Rimoldi, 1980-. “Caracterização biológica e molecular de cepas de Trypanosoma cruzi Chagas, 1909 (Kinetoplastida, Trypanosomatidae) isoladas da Bahia, Rio Grande do Sul, Santa Catarina e São Paulo: Biological and molecular characterization of strains of Trypanosoma cruzi Chagas, 1909 (Kinetoplastida, Trypanosomatidae) isolated from Bahia, Rio Grande do Sul, Santa Catarina and São Paulo.” 2014. Web. 08 Mar 2021.
Vancouver:
Ribeiro, Aline Rimoldi 1. Caracterização biológica e molecular de cepas de Trypanosoma cruzi Chagas, 1909 (Kinetoplastida, Trypanosomatidae) isoladas da Bahia, Rio Grande do Sul, Santa Catarina e São Paulo: Biological and molecular characterization of strains of Trypanosoma cruzi Chagas, 1909 (Kinetoplastida, Trypanosomatidae) isolated from Bahia, Rio Grande do Sul, Santa Catarina and São Paulo. [Internet] [Thesis]. Universidade Estadual de Campinas; 2014. [cited 2021 Mar 08].
Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/317469.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Ribeiro, Aline Rimoldi 1. Caracterização biológica e molecular de cepas de Trypanosoma cruzi Chagas, 1909 (Kinetoplastida, Trypanosomatidae) isoladas da Bahia, Rio Grande do Sul, Santa Catarina e São Paulo: Biological and molecular characterization of strains of Trypanosoma cruzi Chagas, 1909 (Kinetoplastida, Trypanosomatidae) isolated from Bahia, Rio Grande do Sul, Santa Catarina and São Paulo. [Thesis]. Universidade Estadual de Campinas; 2014. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/317469
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
18.
Elizabete Regina Marangoni Marana.
Padronização e avaliação de um teste de Elisa competitivo utilizando a proteína recombinante MSP5-PR1 para o diagnóstico sorológico da anaplasmose bovina.
Degree: 2006, Universidade Estadual de Londrina
URL: http://www.bibliotecadigital.uel.br/document/?code=vtls000115680
► Anaplasma marginale (Rickettsiales: Anaplasmataceae) é o agente etiológico da anaplasmose bovina que se encontra distribuída mundialmente afetando regiões tropicais e subtropicais. É transmitido por vetores,…
(more)
▼ Anaplasma marginale (Rickettsiales: Anaplasmataceae) é o agente etiológico da anaplasmose bovina que se encontra distribuída mundialmente afetando regiões tropicais e subtropicais. É transmitido por vetores, sendo a doença caracterizada clinicamente por febre, anemia hemolítica, perda de peso, aborto e morte. A. marginale possui seis Proteínas Majoritárias de Superfície (MSPs) caracterizadas e designadas MSP1a (105 kDa), MSP1b (100 kDa), MSP2 (36 kDa), MSP3 (86 kDa), MSP4 (31 kDa), MSP5 (19 kDa). Estas proteínas estão envolvidas em importantes eventos entre as células do hospedeiro e o parasita, como respostas humoral e celular, transporte de nutrientes, adesão e invasão de eritrócitos e variação antigênica. A MSP5 é uma proteína altamente conservada entre o isolados estudados de diferentes regiões geográficas, inclusive centrale de Anaplasma centrale e A. ovis. O presente trabalho teve como objetivo padronizar e avaliar um teste imunoenzimático um teste imunoenzimático competitivo (cELISA-PR1), utilizando a rMSP5 do isolado PR1 de A. marginale desenvolvido para o diagnóstico sorológico de anaplasmose. O gene msp5 foi clonado no plasmídio pRSETB e expresso em Escherichia coli BL21 Star (DE3) One Shot (Invitrogen®). O seqüenciamento do gene msp5 mostrou 98% identidade com os isolados Florida e Saint Maries, 97% com isolados Brasil-Pernambuco e Havana; e 91% com A. centrale. A proteína recombinante rMSP5 a partir do isolado PR1 (rMSP5-PR1) foi reconhecida pelo anticorpo de monoclonal ANAF16C1 em Western blotting. O cELISA-PR1 e a IFI foram comparados com cELISA pela análise de 283 soros bovinos, sendo 135 amostras negativas oriundas da região de Santa Vitória do Palmar - RS, e 147 amostras de soros positivas de bovinos naturalmente infectados com A. marginale que obtiveram densidade óptica inferior a 0,490 em 490nm (DO490) através do cELISA foram selecionadas de 245 amostras da região Sul do Brasil. Os testes apresentaram especificidade de 100% e 99,3%, sensibilidade de 100% e 98%, com um coeficiente kappa de 0,993 e 0,978, respectivamente. Na comparação de cELISA-PR1 e IFI com cELISA, a análise de 245 amostras de soros de bovinos naturalmente infectados com A. marginale na região de Sul do Brasil apresentou uma prevalência de 77,55%, 81,63% e 76,73% respectivamente, com especificidade de 96,7% e 98,9%, sensibilidade de 98,9%, 96,3% e um coeficiente kappa de 0,956 e 0,699, respectivamente. Conclui-se, através dos resultados obtidos, que cELISA-PR1 pode ter uso semelhante ao cELISA em testes diagnósticos contra A. marginale durante a infecção aguda, avaliação sorológica, e identificação de bovinos portadores sãos. A soroprevalência de A. marginale foi determinada em 223 amostras de soros de bovinos com dois anos de idade ou mais, da Região de Centro-Sul no Estado de Paraná: região de Ponta Grossa, Guarapuava e Laranjeiras; através da pesquisa de anticorpos da classe IgG contra A. marginale por ensaio imunoenzimático de competição cELISA-PR1. De todos os soros bovinos analisados, 130 (58,74%) reagiram ao cELISA-PR1, uma…
Advisors/Committee Members: Odilon Vidotto ., Rosângela Zacarias Machado, João Luis Garcia, Marilda Carlos Vidotto, Ademir Benedito da Luz Pereira.
Subjects/Keywords: Sorologia veterinária; Anaplasmose; Bovino - Doenças; Veterinary serology; Anaplasmosis; Cattle - Diseases
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APA (6th Edition):
Marana, E. R. M. (2006). Padronização e avaliação de um teste de Elisa competitivo utilizando a proteína recombinante MSP5-PR1 para o diagnóstico sorológico da anaplasmose bovina. (Thesis). Universidade Estadual de Londrina. Retrieved from http://www.bibliotecadigital.uel.br/document/?code=vtls000115680
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Marana, Elizabete Regina Marangoni. “Padronização e avaliação de um teste de Elisa competitivo utilizando a proteína recombinante MSP5-PR1 para o diagnóstico sorológico da anaplasmose bovina.” 2006. Thesis, Universidade Estadual de Londrina. Accessed March 08, 2021.
http://www.bibliotecadigital.uel.br/document/?code=vtls000115680.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Marana, Elizabete Regina Marangoni. “Padronização e avaliação de um teste de Elisa competitivo utilizando a proteína recombinante MSP5-PR1 para o diagnóstico sorológico da anaplasmose bovina.” 2006. Web. 08 Mar 2021.
Vancouver:
Marana ERM. Padronização e avaliação de um teste de Elisa competitivo utilizando a proteína recombinante MSP5-PR1 para o diagnóstico sorológico da anaplasmose bovina. [Internet] [Thesis]. Universidade Estadual de Londrina; 2006. [cited 2021 Mar 08].
Available from: http://www.bibliotecadigital.uel.br/document/?code=vtls000115680.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Marana ERM. Padronização e avaliação de um teste de Elisa competitivo utilizando a proteína recombinante MSP5-PR1 para o diagnóstico sorológico da anaplasmose bovina. [Thesis]. Universidade Estadual de Londrina; 2006. Available from: http://www.bibliotecadigital.uel.br/document/?code=vtls000115680
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
19.
MARQUES, Danielle Medeiros.
Vigilância sobre os contatos de hanseníase no município de João Pessoa: soroprevalência anti-NDO-LID-1 e adesão ao Programa Nacional de Controle da Hanseníase
.
Degree: 2016, Universidade Federal de Pernambuco
URL: http://repositorio.ufpe.br/handle/123456789/17987
► A hanseníase ainda representa um grave problema de Saúde Pública no Brasil, com número absoluto de casos novos atingindo em torno de 30.000/ano. O Programa…
(more)
▼ A hanseníase ainda representa um grave problema de Saúde Pública no Brasil, com
número absoluto de casos novos atingindo em torno de 30.000/ano. O Programa
Nacional de Controle da Hanseníase (PNCH), no sentido de aumentar a detecção e
tratamento precoce da doença, preconiza a avaliação dermatoneurológica e a
vacinação BCG em contatos intradomiciliares de hanseníase. Além desta avaliação
clínica, estudos sorológicos utilizando antígenos específicos do M. leprae vêm
demonstrando alta especificidade, sendo relatada a relação entre a positividade
sorológica nos contatos e o maior risco de adoecimento. O presente estudo teve
como objetivos estimar a soroprevalência anti-NDO-LID-1 em contatos de casos
novos de hanseníase e avaliar a adesão dos mesmos ao PNCH, através de um
estudo transversal e um estudo caso-controle, respectivamente. Metodologia: a
população estudada foram os contatos de casos novos de hanseníase residentes na
cidade de João Pessoa, Paraíba nos anos de 2012 e 2013 (na etapa transversal) e
no ano de 2013 (na etapa caso-controle). Utilizou-se o teste sorológico rápido NDOLID-
1 para aferir a soroprevalência e a adesão ao PNCH foi aferida através da
resposta a um questionário padronizado. Toda a coleta de dados foi domiciliar.
Resultados da etapa transversal: foram identificados 135 casos novos de
hanseníase, com 405 contatos estudados. A soroprevalência foi de 23% (93/405),
sendo maior no sexo feminino (25,9%-58/224), com tempo médio de convívio entre
cinco e 10 anos (44%- 22/50) e naqueles que dormiam no mesmo cômodo e na
mesma cama (26,3%-31/118) do caso índice. Em menores de quinze anos, a
soroprevalência alcançou 27,7% de positividade. Não houve associação da com
variáveis biossociais (faixa etária e sexo), passado de tratamento pra hanseníase,
vacinação BCG e variáveis relacionadas ao caso índice (grau de parentesco,
frequência de contato e tempo de convívio). Resultados da etapa caso-controle:
dentre os 231 contatos, apenas 31 (13,4%) haviam aderido ao PNCH, através da
realização do exame dermatoneurológico. Não foi observada associação significativa
da baixa adesão com as variáveis do contato como sexo, cor da pele, faixa etária,
escolaridade, estado civil, renda familiar, tabagismo, etilismo, uso de drogas ilícitas e
distância do posto de saúde. Observou-se maior adesão ao PNCH dos contatos de
casos de hanseníase com maior idade média e naqueles relacionados às formas
dimorfa e virchowiana. A maioria dos contatos que não aderiram ao Programa
8
Nacional de Controle da Hanseníase relataram como justificativa a desinformação
sobre a necessidade de fazê-lo, fator este determinante para a não adesão ao
programa (OR=0,04, p-valor=0,001). Conclusões: a população de contatos estudada
apresentou uma elevada taxa de soroprevalência e uma baixa adesão ao exame
dermatoneurológico, denotando haver um alta carga bacilar do M. leprae circulante e
uma vigilância sobre os contatos ainda negligenciada.
Advisors/Committee Members: XIMENES, Ricardo Arraes de Alencar (advisor), http://lattes.cnpq.br/8153478596620105 (advisor).
Subjects/Keywords: Hanseníase;
Sorologia;
Contatos;
Vigilância;
Leprosy;
Serology;
Contacts;
Surveillance
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APA ·
Chicago ·
MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
MARQUES, D. M. (2016). Vigilância sobre os contatos de hanseníase no município de João Pessoa: soroprevalência anti-NDO-LID-1 e adesão ao Programa Nacional de Controle da Hanseníase
. (Thesis). Universidade Federal de Pernambuco. Retrieved from http://repositorio.ufpe.br/handle/123456789/17987
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
MARQUES, Danielle Medeiros. “Vigilância sobre os contatos de hanseníase no município de João Pessoa: soroprevalência anti-NDO-LID-1 e adesão ao Programa Nacional de Controle da Hanseníase
.” 2016. Thesis, Universidade Federal de Pernambuco. Accessed March 08, 2021.
http://repositorio.ufpe.br/handle/123456789/17987.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
MARQUES, Danielle Medeiros. “Vigilância sobre os contatos de hanseníase no município de João Pessoa: soroprevalência anti-NDO-LID-1 e adesão ao Programa Nacional de Controle da Hanseníase
.” 2016. Web. 08 Mar 2021.
Vancouver:
MARQUES DM. Vigilância sobre os contatos de hanseníase no município de João Pessoa: soroprevalência anti-NDO-LID-1 e adesão ao Programa Nacional de Controle da Hanseníase
. [Internet] [Thesis]. Universidade Federal de Pernambuco; 2016. [cited 2021 Mar 08].
Available from: http://repositorio.ufpe.br/handle/123456789/17987.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
MARQUES DM. Vigilância sobre os contatos de hanseníase no município de João Pessoa: soroprevalência anti-NDO-LID-1 e adesão ao Programa Nacional de Controle da Hanseníase
. [Thesis]. Universidade Federal de Pernambuco; 2016. Available from: http://repositorio.ufpe.br/handle/123456789/17987
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of South Africa
20.
Etsebeth, Charné.
A serological survey to determine the prevalence of Brucella Canis infection in dogs within the Nelson Mandela Bay metropolitan in the Eastern Cape, South Africa
.
Degree: 2017, University of South Africa
URL: http://hdl.handle.net/10500/23286
► The prevalence of Brucella canis in South Africa is unknown and suspected to be under-detected. The majority of dogs in South Africa are not tested…
(more)
▼ The prevalence of Brucella canis in South Africa is unknown and suspected to be under-detected. The majority of dogs in South Africa are not tested for Brucella canis, not only because of the level of awareness of Brucella canis in South Africa, but also because of the lack of clinical suspicion. It is not known how the infection entered South Africa.
Brucella canis, a zoonotic organism that causes canine brucellosis in dogs, is a significant cause of reproductive failure in dogs worldwide. Canine brucellosis is a chronic infectious zoonotic disease whose main etiological agent, the Brucella canis bacterium, are rough, intracellular proteobacteria in the Brucellaceae family. Clinical signs in bitches are mainly infertility and abortion, while in males, epididymitis and orchitis occur. However, discospondylitis may develop in both sexes.
A serological survey was conducted to determine the prevalence of Brucella canis infection in dogs from the Nelson Mandela Bay Metropolitan (NMBM) Port Elizabeth (PE) area. A total of 400 samples were collected, 350 of which were collected in seven different townships and 50 were collected in the three different welfare organisations in the study area.
Of the 400 serum samples collected, 39 (9.75%) tested serologically positive by using the Tube Agglutination Test (TAT), the 2-Mercaptoethanol-TAT (2ME-TAT) or the Compliment Fixation Test (CFT). The results of the CFT showed that nine of the 39 positive samples had a maximum antibody titre of 784 IU/ml. The prevalence rate varied tremendously between the samples from the townships and those from the welfare organisations. The prevalence rate of seropositive animals in PE ranged between 5% and 16% in the study area. No positive cases were found in KwaMagxaki and the Animal Welfare Society of PE, but both were surrounded by areas that had positive cases of Brucella canis infection.
The female dogs in the study area had a higher sero-prevalence of only 0.0169 (95% CI, 0.0631 to 0.1489) differences in proportion, and were thus not significant (p > 0.05). However, the female spayed dogs had a much higher significant difference of 0.1898 (95%
iv
CI, 0.1058 to 0.2738) in proportion to the male neutered dogs and were thus statistically significant (p < 0.05). Despite those results, out of all the dogs positive for Brucella canis only two were neutered males and five were spayed females, and the rest were all intact.
In conclusion, according to the results, Brucella canis antibodies were detected in sera of dogs mostly from the townships surveyed. Preventive measures against this contagion should be taken into consideration to eliminate Brucella canis infection from the entire dog population. Reservoir dogs and actively infected dogs either should be kept in quarantine or should be euthanized, because not only can they spread the disease and end the reproductive life of any breeding animal, but they are also a risk to human health.
Even though this is the first survey conducted in the Eastern Cape, the results are still high
dogs in…
Advisors/Committee Members: Oosthuizen, J (advisor), Sibanda, D.R (advisor).
Subjects/Keywords: Zoonotic disease;
Humans;
Abortion;
Infertility;
Townships;
Welfare organisations;
Serology;
TAT;
CFT
Record Details
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Record Details
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Etsebeth, C. (2017). A serological survey to determine the prevalence of Brucella Canis infection in dogs within the Nelson Mandela Bay metropolitan in the Eastern Cape, South Africa
. (Masters Thesis). University of South Africa. Retrieved from http://hdl.handle.net/10500/23286
Chicago Manual of Style (16th Edition):
Etsebeth, Charné. “A serological survey to determine the prevalence of Brucella Canis infection in dogs within the Nelson Mandela Bay metropolitan in the Eastern Cape, South Africa
.” 2017. Masters Thesis, University of South Africa. Accessed March 08, 2021.
http://hdl.handle.net/10500/23286.
MLA Handbook (7th Edition):
Etsebeth, Charné. “A serological survey to determine the prevalence of Brucella Canis infection in dogs within the Nelson Mandela Bay metropolitan in the Eastern Cape, South Africa
.” 2017. Web. 08 Mar 2021.
Vancouver:
Etsebeth C. A serological survey to determine the prevalence of Brucella Canis infection in dogs within the Nelson Mandela Bay metropolitan in the Eastern Cape, South Africa
. [Internet] [Masters thesis]. University of South Africa; 2017. [cited 2021 Mar 08].
Available from: http://hdl.handle.net/10500/23286.
Council of Science Editors:
Etsebeth C. A serological survey to determine the prevalence of Brucella Canis infection in dogs within the Nelson Mandela Bay metropolitan in the Eastern Cape, South Africa
. [Masters Thesis]. University of South Africa; 2017. Available from: http://hdl.handle.net/10500/23286
21.
Piarroux, Raphaël.
Développement de tests de diagnostic in vitro appliqués au sérodiagnostic des infections fongiques par western blot et immunochromatographie : Development of in vitro diagnostic test applied to the diagnosis of fungal infections by western blot and immunochromatography.
Degree: Docteur es, Biologie. Microbiologie, 2018, Aix Marseille Université
URL: http://www.theses.fr/2018AIXM0763
► Le champignon microscopique Aspergillus fumigatus provoque un nombre important de maladies graves. Parmi elles, l’aspergillose pulmonaire chronique (APC) et l’aspergillose broncho-pulmonaire allergique (ABPA) affectent 3…
(more)
▼ Le champignon microscopique Aspergillus fumigatus provoque un nombre important de maladies graves. Parmi elles, l’aspergillose pulmonaire chronique (APC) et l’aspergillose broncho-pulmonaire allergique (ABPA) affectent 3 et 4,8 millions de personnes dans le monde, respectivement.L’APC est très souvent mortelle si elle n’est pas soignée. Elle se développe très souvent après une tuberculose. C’est donc une maladie des pays émergents, où il n’est souvent pas possible de la diagnostiquer à cause du coût trop important des techniques existantes.L’ABPA est une complication très grave de l’asthme et de la mucoviscidose, qui complique fortement ces maladies. Elle est très difficile à diagnostiquer.Notre travail a donc consisté à développer et évaluer deux tests, un test rapide permettant de poser le diagnostic d’APC sans avoir à utiliser de matériel de laboratoire à destination des pays émergents et un western blot qui permet la confirmation du diagnostic d’ABPA.
Aspergillus fumigatus is a microscopic fungus that can cause numerous diseases. Among them, chronic pulmonary aspergillosis (CPA) and allergic broncho-pulmonary aspergilloses (ABPA) affect 3 and 4.8 million people, respectively.CPA is often fatal if left untreated. It is often a complication of tuberculosis and therefore affect low and middle income countries. However, it is difficult to diagnose it in those countries, as the tests are too expansive.ABPA is a severe complication of asthma and cystic fibrosis, worsening those diseases. It’s very hard to diagnose it.Our work was to develop and evaluate two tests, a rapid test for the diagnosis of CPA that does not require laboratory equipment designed for low and middle income countries and a western blot for confirmation of ABPA diagnosis.
Advisors/Committee Members: Ranque, Stéphane (thesis director).
Subjects/Keywords: Western blot; Immunochromatographie; Aspergillus; Sérologie; Aspergillus; Western blot; Immunochromatography; Serology
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APA ·
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MLA ·
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CSE |
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to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Piarroux, R. (2018). Développement de tests de diagnostic in vitro appliqués au sérodiagnostic des infections fongiques par western blot et immunochromatographie : Development of in vitro diagnostic test applied to the diagnosis of fungal infections by western blot and immunochromatography. (Doctoral Dissertation). Aix Marseille Université. Retrieved from http://www.theses.fr/2018AIXM0763
Chicago Manual of Style (16th Edition):
Piarroux, Raphaël. “Développement de tests de diagnostic in vitro appliqués au sérodiagnostic des infections fongiques par western blot et immunochromatographie : Development of in vitro diagnostic test applied to the diagnosis of fungal infections by western blot and immunochromatography.” 2018. Doctoral Dissertation, Aix Marseille Université. Accessed March 08, 2021.
http://www.theses.fr/2018AIXM0763.
MLA Handbook (7th Edition):
Piarroux, Raphaël. “Développement de tests de diagnostic in vitro appliqués au sérodiagnostic des infections fongiques par western blot et immunochromatographie : Development of in vitro diagnostic test applied to the diagnosis of fungal infections by western blot and immunochromatography.” 2018. Web. 08 Mar 2021.
Vancouver:
Piarroux R. Développement de tests de diagnostic in vitro appliqués au sérodiagnostic des infections fongiques par western blot et immunochromatographie : Development of in vitro diagnostic test applied to the diagnosis of fungal infections by western blot and immunochromatography. [Internet] [Doctoral dissertation]. Aix Marseille Université 2018. [cited 2021 Mar 08].
Available from: http://www.theses.fr/2018AIXM0763.
Council of Science Editors:
Piarroux R. Développement de tests de diagnostic in vitro appliqués au sérodiagnostic des infections fongiques par western blot et immunochromatographie : Development of in vitro diagnostic test applied to the diagnosis of fungal infections by western blot and immunochromatography. [Doctoral Dissertation]. Aix Marseille Université 2018. Available from: http://www.theses.fr/2018AIXM0763
Cape Peninsula University of Technology
22.
Motswaledi, Modisa Sekhamo.
The role of blood groups in preventing or enhancing HIV infection in Botswana
.
Degree: 2019, Cape Peninsula University of Technology
URL: http://etd.cput.ac.za/handle/20.500.11838/2813
► Knowledge of population vulnerabilities to infectious diseases is key in managing many public health problems and for mapping appropriate strategies for prevention or intervention. A…
(more)
▼ Knowledge of population vulnerabilities to infectious diseases is key in managing many public health problems and for mapping appropriate strategies for prevention or intervention. A number of genes associated with resistance to HIV infection, such as the double deletion of 32 base pairs in the CCR5 gene , have been described and potentially account for lower HIV infections in some populations. The magnitude of the HIV pandemic in Sub-Saharan Africa warrants an investigation of the peculiar genetic factors that may have exacerbated its spread. An understanding of the genetic factors that are involved may aid in the development of specific strategies for prevention such as vaccine development, genetic counselling as well as gene therapy. The aim of this project was therefore to study the relationship between blood groups and HIV-infection in Botswana. HIV infection in Africa has not been linked to particular blood groups. The project was undertaken in two phases from December 2012 to December 2017. In the first phase, 346 subjects of known HIV status (negative or positive) were phenotyped for 23 erythrocyte antigens via standard scientific procedures. A Chi-square analysis was used to determine those antigens associated with increased or reduced risk of HIV infection. In the second phase, 120 samples were phenotyped for the protective blood group (RhC) and the risk-associated groups (Lub and P1). The samples were also characterized according to their laboratory results for viral load, lymphocyte sub-populations, complete blood count and blood chemistry, including total cholesterol. Some of the samples were also assessed for erythrocyte-associated viral RNA. Generally, the prevalence of the blood groups in the general population in Botswana did not differ with the known prevalence for Africans broadly. Three novel findings were established. First, the blood group Rh(C) was associated with a 40% risk reduction for HIV infection. Immunologically, carriage of the C antigen was associated with a more robust cell-mediated immunity as evidenced by enhanced cytotoxic T cell counts. Moreover, this antigen occurred with a frequency lower than 30% in all countries where HIV prevalence was high. There was therefore an inverse relationship between Rh(C) frequency and HIV prevalence. An examination of reports from previous studies revealed that the pattern was consistent in Africa, Europe, Asia, South America and Caribbean countries. It appears that the population frequency of this antigen explains, at least in part, a genetic factor that puts some African populations at higher risk for HIV infection. These results are novel in that Rh antigens have not been previously associated with immunity in any reports.
Novel findings regarding the P1 blood group was its association with a double risk for HIV infection. While the plasma viral load did not differ between P1-positive and P1-negative subjects, P1-positive erythrocyte lysate yielded more viral RNA than P1-negative cells, implying more intracellular HIV RNA. Intra-erythrocytic…
Subjects/Keywords: HIV (Viruses);
HIV infections;
Blood groups;
Serology;
Medical genetics
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APA ·
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MLA ·
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Manager
APA (6th Edition):
Motswaledi, M. S. (2019). The role of blood groups in preventing or enhancing HIV infection in Botswana
. (Thesis). Cape Peninsula University of Technology. Retrieved from http://etd.cput.ac.za/handle/20.500.11838/2813
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Motswaledi, Modisa Sekhamo. “The role of blood groups in preventing or enhancing HIV infection in Botswana
.” 2019. Thesis, Cape Peninsula University of Technology. Accessed March 08, 2021.
http://etd.cput.ac.za/handle/20.500.11838/2813.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Motswaledi, Modisa Sekhamo. “The role of blood groups in preventing or enhancing HIV infection in Botswana
.” 2019. Web. 08 Mar 2021.
Vancouver:
Motswaledi MS. The role of blood groups in preventing or enhancing HIV infection in Botswana
. [Internet] [Thesis]. Cape Peninsula University of Technology; 2019. [cited 2021 Mar 08].
Available from: http://etd.cput.ac.za/handle/20.500.11838/2813.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Motswaledi MS. The role of blood groups in preventing or enhancing HIV infection in Botswana
. [Thesis]. Cape Peninsula University of Technology; 2019. Available from: http://etd.cput.ac.za/handle/20.500.11838/2813
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Boston University
23.
Rogers, Caitlin Eileen.
The prevalence of intact spermatozoa on intimate smear and extract slides: a retrospective case review and re-evaluation of time since intercourse estimation.
Degree: MS, Biomedical Forensic Sciences, 2014, Boston University
URL: http://hdl.handle.net/2144/14276
► Literature concerning the time frames for detection of various seminal components commonly tested for in forensic laboratories in sexual assault cases is limited in quantity…
(more)
▼ Literature concerning the time frames for detection of various seminal components commonly tested for in forensic laboratories in sexual assault cases is limited in quantity and in scope. Determining a more accurate time since intercourse (TSI) interval based on an extensive review of forensic case work would provide investigators with a tool for estimating the time elapsed between the occurrence of a sexual assault and the collection of a Sexual Assault Evidence Collection Kit (SAECK) which could be vital information in certain cases. This study demonstrates that the presence of intact spermatozoa is a significant finding on microscope slides prepared from vaginal, anorectal, and oral swabs and that the percentage of intact sperm cells decreases over time. This study also proved that sperm tails are lost during the preparation of microscope slides from SAECK swabs by directly comparing medical personnel-prepared smear slides and analyst-prepared extract slides from 95 Boston Police Department (BPD) Crime Laboratory Unit cases. Additionally, this study presents maximum TSI values for the persistence of sperm heads, intact spermatozoa, and prostate specific antigen (P30) through a retrospective examination of 355 cases processed by the BPD Crime Laboratory Unit over 5 years. The maximum persistence values for P30 in the vaginal, anorectal, and oral cavities were 19 hours, 17 hours, and 20 hours, respectively. In the vaginal cavity, maximum persistence values for intact spermatozoa were 43 hours for smear slides and 41.5 hours for extract slides. The maximum persistence of sperm heads was equivalent for vaginal smear and extract slides at 105 hours. In the anorectal cavity, maximum persistence values for intact spermatozoa were 43 hours for smear slides and 13 hours for extract slides. The maximum persistence of sperm heads was equivalent for anorectal smear and extract slides at 43 hours. In the oral cavity, maximum persistence values for intact spermatozoa were 3.75 hours for smear slides and 5 hours for extract slides. The maximum persistence of sperm heads were equivalent for oral smear and extract slides at 24 hours.
Subjects/Keywords: Biology; Forensics; Semen; Serology; Spermatozoa; Sexual assault; Time since intercourse
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APA (6th Edition):
Rogers, C. E. (2014). The prevalence of intact spermatozoa on intimate smear and extract slides: a retrospective case review and re-evaluation of time since intercourse estimation. (Masters Thesis). Boston University. Retrieved from http://hdl.handle.net/2144/14276
Chicago Manual of Style (16th Edition):
Rogers, Caitlin Eileen. “The prevalence of intact spermatozoa on intimate smear and extract slides: a retrospective case review and re-evaluation of time since intercourse estimation.” 2014. Masters Thesis, Boston University. Accessed March 08, 2021.
http://hdl.handle.net/2144/14276.
MLA Handbook (7th Edition):
Rogers, Caitlin Eileen. “The prevalence of intact spermatozoa on intimate smear and extract slides: a retrospective case review and re-evaluation of time since intercourse estimation.” 2014. Web. 08 Mar 2021.
Vancouver:
Rogers CE. The prevalence of intact spermatozoa on intimate smear and extract slides: a retrospective case review and re-evaluation of time since intercourse estimation. [Internet] [Masters thesis]. Boston University; 2014. [cited 2021 Mar 08].
Available from: http://hdl.handle.net/2144/14276.
Council of Science Editors:
Rogers CE. The prevalence of intact spermatozoa on intimate smear and extract slides: a retrospective case review and re-evaluation of time since intercourse estimation. [Masters Thesis]. Boston University; 2014. Available from: http://hdl.handle.net/2144/14276
24.
Palache, Abraham.
Influenza vaccination : the effect of dose and age on the antibody response : a methodological evaluation of serological vaccination studies.
Degree: 1991, Erasmus University Medical Center
URL: http://hdl.handle.net/1765/40852
► textabstractConsidering the development of new influenza vaccines, some fundamental questions need to be addressed, some of which are listed below: 1) What antigen-specific and cross-reactive…
(more)
▼ textabstractConsidering the development of new influenza vaccines, some fundamental
questions need to be addressed, some of which are listed below:
1) What antigen-specific and cross-reactive immunological responses should they
minimally induce?
2) What type of clinical studies (field-, artificial challenge- and serological studies)
would be required to prove the new vaccine's efficacy and/or its superiority to the
existing influenza vaccines?
3) Which .. parameters in serological and/or efficacy studies are the most appropriate
markers to assess vaccine efficacy?
4) What criteria could be used as relevant yardsticks to make a final assessment of a
new influenza vaccine?
These and similar methodological questions were the rationale behind the work presented
in this thesis. For competitive reasons, the strategic consequences of this work
for future influenza vaccine development are not discussed. Some proposals, however,
are presented to define internationally accepted consensus criteria to quantitatively
evaluate serological influenza vaccination studies.
Obviously, the studies presented in this thesis could only be accomplished by the
efforts of literally hundreds of people, such as the study participants, the investigators,
co-authors, own and consultant statisticians, members of Duphar's influenza project group, laboratory technicians, secretaries, data-handlers, library staff, editor,
Duphar's European National Organisations, colleagues at the Clinical Research
Department, the Department of Biotechnology, the International Medical
Department, the Adverse Drug Reaction Unit the Department of Information
Management and, last but not least, the Department of Virology of the Erasmus
University. I am grateful to all these people for their contribution to the work contained
in this thesis.
Subjects/Keywords: immune reactions; influenza; serology; vaccination
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APA (6th Edition):
Palache, A. (1991). Influenza vaccination : the effect of dose and age on the antibody response : a methodological evaluation of serological vaccination studies. (Doctoral Dissertation). Erasmus University Medical Center. Retrieved from http://hdl.handle.net/1765/40852
Chicago Manual of Style (16th Edition):
Palache, Abraham. “Influenza vaccination : the effect of dose and age on the antibody response : a methodological evaluation of serological vaccination studies.” 1991. Doctoral Dissertation, Erasmus University Medical Center. Accessed March 08, 2021.
http://hdl.handle.net/1765/40852.
MLA Handbook (7th Edition):
Palache, Abraham. “Influenza vaccination : the effect of dose and age on the antibody response : a methodological evaluation of serological vaccination studies.” 1991. Web. 08 Mar 2021.
Vancouver:
Palache A. Influenza vaccination : the effect of dose and age on the antibody response : a methodological evaluation of serological vaccination studies. [Internet] [Doctoral dissertation]. Erasmus University Medical Center; 1991. [cited 2021 Mar 08].
Available from: http://hdl.handle.net/1765/40852.
Council of Science Editors:
Palache A. Influenza vaccination : the effect of dose and age on the antibody response : a methodological evaluation of serological vaccination studies. [Doctoral Dissertation]. Erasmus University Medical Center; 1991. Available from: http://hdl.handle.net/1765/40852
Montana State University
25.
Liebelt, James Edward.
A serological study of cutthroat trout (Salmo clarki) from tributaries and the outlet of Yellowstone Lake.
Degree: MS, College of Agriculture, 1968, Montana State University
URL: https://scholarworks.montana.edu/xmlui/handle/1/5609
Subjects/Keywords: Cutthroat trout.; Fishes.; Serology.
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APA (6th Edition):
Liebelt, J. E. (1968). A serological study of cutthroat trout (Salmo clarki) from tributaries and the outlet of Yellowstone Lake. (Masters Thesis). Montana State University. Retrieved from https://scholarworks.montana.edu/xmlui/handle/1/5609
Chicago Manual of Style (16th Edition):
Liebelt, James Edward. “A serological study of cutthroat trout (Salmo clarki) from tributaries and the outlet of Yellowstone Lake.” 1968. Masters Thesis, Montana State University. Accessed March 08, 2021.
https://scholarworks.montana.edu/xmlui/handle/1/5609.
MLA Handbook (7th Edition):
Liebelt, James Edward. “A serological study of cutthroat trout (Salmo clarki) from tributaries and the outlet of Yellowstone Lake.” 1968. Web. 08 Mar 2021.
Vancouver:
Liebelt JE. A serological study of cutthroat trout (Salmo clarki) from tributaries and the outlet of Yellowstone Lake. [Internet] [Masters thesis]. Montana State University; 1968. [cited 2021 Mar 08].
Available from: https://scholarworks.montana.edu/xmlui/handle/1/5609.
Council of Science Editors:
Liebelt JE. A serological study of cutthroat trout (Salmo clarki) from tributaries and the outlet of Yellowstone Lake. [Masters Thesis]. Montana State University; 1968. Available from: https://scholarworks.montana.edu/xmlui/handle/1/5609
University of Lund
26.
Faust, Helena.
Pseudovirions in the Study of Papilloma- and
Polyomaviruses.
Degree: 2012, University of Lund
URL: https://lup.lub.lu.se/record/3216297
;
https://portal.research.lu.se/ws/files/3451011/3216441.pdf
► The papilloma- and polyomaviruses are small DNA viruses that infect humans. Some members of these virus families can cause cancer in experimental animals and some…
(more)
▼ The papilloma- and polyomaviruses are small DNA
viruses that infect humans. Some members of these virus families
can cause cancer in experimental animals and some are also
established as carcinogenic to humans. Detection of specific
antibodies to these viruses allows tracking past and present
infections to elucidate natural history and association of
infection with subsequent disease. As there are more than 150 types
of Human papillomaviruses (HPV) and at least 10 Human
polyomaviruses (HPyV), the study of antibodies to these virus
families require high-throughput methods. Although it is difficult
to produce infectious virus stocks of these viruses, so-called
pseudovirions that morphologically and immunologically resemble
native virions but lack the viral genome can be produced in vitro.
In the present thesis, we produced such pseudovirions and used them
to i) delineate the importance of hypervariable surface loops for
the antigenicity and biological function of the HPV particle ii)
developed and validate serological assays for measuring specific
antibodies to HPV and Merkel cell polyomavirus (MCPyV) and iii)
perform prospective seroepidemio-logical studies to evaluate
whether infection with MCPyV was associated with an increased risk
for Merkel cell carcinoma. Site-directed mutagenesis of the surface
loops of the HPV capsid found that these loops were essential for
the incorporation of the minor capsid protein L2, the genome
encapsidation and proper immunogenicity of the particle.
Pseudovirion-based methods were correlated to presence of viral
DNA. The pseudovirion neutralization assays and multiplexed assays
using pseudovirions bound to heparin-coated fluorescent beads for
21 HPV and 2 HPyV types were correlated with viral DNA for 16 HPV
types and MCPyV. MCPyV specific antibody levels in serum were found
to be strongly correlated to the MCPyV viral load in skin. Finally,
biobank-based seroepidemiological studies found that MCPyV
infection was associated with an increased risk for Merkel cell
carcinoma (MCC), in particular among females.
Subjects/Keywords: Microbiology in the medical area; Human papilloma- and polyomaviruses; pseudovirions; serology
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APA ·
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MLA ·
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CSE |
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APA (6th Edition):
Faust, H. (2012). Pseudovirions in the Study of Papilloma- and
Polyomaviruses. (Doctoral Dissertation). University of Lund. Retrieved from https://lup.lub.lu.se/record/3216297 ; https://portal.research.lu.se/ws/files/3451011/3216441.pdf
Chicago Manual of Style (16th Edition):
Faust, Helena. “Pseudovirions in the Study of Papilloma- and
Polyomaviruses.” 2012. Doctoral Dissertation, University of Lund. Accessed March 08, 2021.
https://lup.lub.lu.se/record/3216297 ; https://portal.research.lu.se/ws/files/3451011/3216441.pdf.
MLA Handbook (7th Edition):
Faust, Helena. “Pseudovirions in the Study of Papilloma- and
Polyomaviruses.” 2012. Web. 08 Mar 2021.
Vancouver:
Faust H. Pseudovirions in the Study of Papilloma- and
Polyomaviruses. [Internet] [Doctoral dissertation]. University of Lund; 2012. [cited 2021 Mar 08].
Available from: https://lup.lub.lu.se/record/3216297 ; https://portal.research.lu.se/ws/files/3451011/3216441.pdf.
Council of Science Editors:
Faust H. Pseudovirions in the Study of Papilloma- and
Polyomaviruses. [Doctoral Dissertation]. University of Lund; 2012. Available from: https://lup.lub.lu.se/record/3216297 ; https://portal.research.lu.se/ws/files/3451011/3216441.pdf
Univerzitet u Beogradu
27.
Aničić, Milan, 1985-, 25072743.
Анализа морфолошких промена, експресије и дистрибуције
вирусног антигена у мозгу лисица природно инфицираних вирусом
штенећака.
Degree: Fakultet veterinarske medicine, 2020, Univerzitet u Beogradu
URL: https://fedorabg.bg.ac.rs/fedora/get/o:21056/bdef:Content/get
► Ветеринарска медицина - Патологија / Veterinary medicine - Pathology
У овој докторској дисертацији испитивани су узорци мозга црвених лисица (Vulpes vulpes) природно инфицираних вирусом штенећака.…
(more)
▼ Ветеринарска медицина - Патологија / Veterinary
medicine - Pathology
У овој докторској дисертацији испитивани су узорци
мозга црвених лисица (Vulpes vulpes) природно инфицираних вирусом
штенећака. Након искључивања животиња позитивних на беснило методом
директне имунофлуоресценције, вршена су испитивања узорака серума
црвених лисица имуноензимском методом у циљу утврђивања присуства
антитела против вируса штенећака. Рађена су макроскопска и
микроскопска испитивања узорака мозга серолошки позитивних јединки.
Експресија и дистрибуција вирусног антигена у мозгу инфицираних
лисица утврђена је имунохистохемијски, као и испитивање природе
инфламаторног ћелијског инфилтрата. Хистохемијским бојењем LFB
методом одређен је степен демијелинизације беле мождане масе.
Молекуларно-генетичка испитивања коришћена су за доказивање вирусне
РНК. Извршена је статистичка обрада резултата утврђених
патоморфолошких промена. Није утврђено присуство јединки позитивних
на беснило. Присуство антитела утврђено је код 36,8% животиња и
није утврђен утицај висине титра антитела на израженост
патоморфолошких промена. Иако нису утврђене макроскопске промене,
микроскопски су се уочавале бројне промене у типу негнојног
паненцефалитиса и демијелинизујућег леукоенцефалитиса.
Имунохистохемијски, утврђена је позитивна реакција умереног до
јаког интензитета против вирусног нуклеопротеина (CDV-NP), као и
присуство Т ћелија у периваскуларном инфилтрату. Демијелинизација
је била најизраженија у белој маси малог мозга. Употребом
молекуларно-генетичке методе (RT-PCR) није утврђено присуство
вирусне РНК. Поређењем средњих вредности хистопатолошких промена
статистичком обрадом података утврђена је сигнификантна разлика
између појединих сегмената мозга.
Advisors/Committee Members: Marinković, Darko, 1975-, 12894567.
Subjects/Keywords: red fox; Vulpes vulpes; distemper; histopathology;
immunohistochemistry; CDV-NP; serology
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APA (6th Edition):
Aničić, Milan, 1985-, 2. (2020). Анализа морфолошких промена, експресије и дистрибуције
вирусног антигена у мозгу лисица природно инфицираних вирусом
штенећака. (Thesis). Univerzitet u Beogradu. Retrieved from https://fedorabg.bg.ac.rs/fedora/get/o:21056/bdef:Content/get
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Aničić, Milan, 1985-, 25072743. “Анализа морфолошких промена, експресије и дистрибуције
вирусног антигена у мозгу лисица природно инфицираних вирусом
штенећака.” 2020. Thesis, Univerzitet u Beogradu. Accessed March 08, 2021.
https://fedorabg.bg.ac.rs/fedora/get/o:21056/bdef:Content/get.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Aničić, Milan, 1985-, 25072743. “Анализа морфолошких промена, експресије и дистрибуције
вирусног антигена у мозгу лисица природно инфицираних вирусом
штенећака.” 2020. Web. 08 Mar 2021.
Vancouver:
Aničić, Milan, 1985- 2. Анализа морфолошких промена, експресије и дистрибуције
вирусног антигена у мозгу лисица природно инфицираних вирусом
штенећака. [Internet] [Thesis]. Univerzitet u Beogradu; 2020. [cited 2021 Mar 08].
Available from: https://fedorabg.bg.ac.rs/fedora/get/o:21056/bdef:Content/get.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Aničić, Milan, 1985- 2. Анализа морфолошких промена, експресије и дистрибуције
вирусног антигена у мозгу лисица природно инфицираних вирусом
штенећака. [Thesis]. Univerzitet u Beogradu; 2020. Available from: https://fedorabg.bg.ac.rs/fedora/get/o:21056/bdef:Content/get
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Melbourne
28.
Prideaux, Lani.
Inflammatory bowel disease in the East and West: clinical, serological and microbiological studies.
Degree: 2013, University of Melbourne
URL: http://hdl.handle.net/11343/38302
► The incidence of inflammatory bowel disease (IBD) in Australia is equal to the highest in the world, in contrast to the incidence in Asia which…
(more)
▼ The incidence of inflammatory bowel disease (IBD) in Australia is equal to the highest in the world, in contrast to the incidence in Asia which is low but rapidly increasing. The pathophysiology of IBD relates to the mucosal immune response to antigenicstimulation from the gut microbiota, on a background of genetic susceptibility. Immigrants from low to high incidence areas, have a high incidence of IBD, suggesting that exposure to new environmental factors is a key factor in the development of IBD. “Westernization” of lifestyle and industrialization in Asia likely also plays a role.
Our gut microbiota is affected by our genes, our immune system, and environmental factors. Patients with IBD have altered gut microbiota. Little is known about the gut microbiota of IBD patients in the Asia, and in particular whether it is changing to Western patterns, especially after migration.
Initially the clinical characteristics and management of IBD patients in Australia and Asia were compared. Then gut microbiota was assessed and compared, using state-of-the-art metagenomic techniques, within and between China and Australia (countries with different IBD incidence) both in the healthy, and IBD populations in subjects of Caucasian and Asian (Chinese) ethnicity. Serological antibodies to microbial antigens, and environmental factors were also assessed.
Studying clinical characteristics of disease, the gut microbiota and serological antibodies to microbial antigens in populations with changing incidence offers great hope of identifying potentially important aetiological factors in IBD.
Subjects/Keywords: inflammatory bowel disease; Crohn's disease; ulcerative colitis; microbiota; ethnic; Asia; serology
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APA (6th Edition):
Prideaux, L. (2013). Inflammatory bowel disease in the East and West: clinical, serological and microbiological studies. (Doctoral Dissertation). University of Melbourne. Retrieved from http://hdl.handle.net/11343/38302
Chicago Manual of Style (16th Edition):
Prideaux, Lani. “Inflammatory bowel disease in the East and West: clinical, serological and microbiological studies.” 2013. Doctoral Dissertation, University of Melbourne. Accessed March 08, 2021.
http://hdl.handle.net/11343/38302.
MLA Handbook (7th Edition):
Prideaux, Lani. “Inflammatory bowel disease in the East and West: clinical, serological and microbiological studies.” 2013. Web. 08 Mar 2021.
Vancouver:
Prideaux L. Inflammatory bowel disease in the East and West: clinical, serological and microbiological studies. [Internet] [Doctoral dissertation]. University of Melbourne; 2013. [cited 2021 Mar 08].
Available from: http://hdl.handle.net/11343/38302.
Council of Science Editors:
Prideaux L. Inflammatory bowel disease in the East and West: clinical, serological and microbiological studies. [Doctoral Dissertation]. University of Melbourne; 2013. Available from: http://hdl.handle.net/11343/38302
University of Melbourne
29.
Hamilton, Amy Louise.
Immunological and microbiological studies in post-operative Crohn's disease.
Degree: 2017, University of Melbourne
URL: http://hdl.handle.net/11343/211688
► Crohn’s disease is a chronic, inflammatory condition of the bowel. The aetiology of Crohn’s has not been fully elucidated, but is believed to arise from…
(more)
▼ Crohn’s disease is a chronic, inflammatory condition of the bowel. The aetiology of Crohn’s has not been fully elucidated, but is believed to arise from the interaction between the gut microbiota, the host immune system and environmental factors. A majority of Crohn’s disease patients will require a bowel resection as a result of disease, which causes significant morbidity and impacts on quality of life. While surgery can ameliorate clinical symptoms, the disease often initially recurs at the site of the resection. This may be sub-clinical initially, and can be identified endoscopically.
This thesis investigates the immunological and microbiological characteristics of post-operative Crohn’s disease recurrence in, addressing serologic markers, the faecal microbiome and the possible contribution of Proteus species to gastrointestinal disease.
Serologic markers, while disappointing for prediction of disease recurrence, did have some utility for identifying patients at the highest risk of disease recurrence. I also demonstrated lower rates of antibody positivity in Crohn’s patients who smoke for the first time, indicating that these results should be interpreted with caution in current and past smokers.
I have further evaluated the faecal microbiome in the setting of post-operative disease using metagenomic techniques. This work showed that after resection for Crohn's disease, enrichment of the bacterial family Lachnospiraceae is associated with maintenance of disease remission, while patients enriched for the Enterobacteriaceae are more likely to recur. Recurrence may result from a higher abundance of enteric pathobionts such as Proteus, Klebsiella, Serratia, and Escherichia. The Lachnospiraceae are an important family of butyrate producing bacteria in the gut, and depletion of this bacterial family may perpetuate the expansion of Enterobacteriaceae via environmental perturbation and ecologic shifts. These findings indicate possible protective and pathogenic bacteria in post-operative recurrence.
Finally, I addressed the potential contribution of Proteus species (from the Enterobacteriaceae family) to gastrointestinal diseases including to inflammatory bowel disease. Proteus spp. are low-abundance commensals of the human gut that harbour significant pathogenic potential. Preliminary evidence of a pathogenic role in the gut should stimulate further investigation.
I have elucidated some aspects of the microbiome and host immune factors involved, in the pathophysiology of post-operative Crohn’s disease recurrence. This work should encourage further work on a unifying hypothesis for the aetiology of disease recurrence after resectional surgery for Crohn’s disease.
Subjects/Keywords: Crohn's disease; microbiota; post-operative; serology; Enterobacteriaceae; Proteus
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APA (6th Edition):
Hamilton, A. L. (2017). Immunological and microbiological studies in post-operative Crohn's disease. (Doctoral Dissertation). University of Melbourne. Retrieved from http://hdl.handle.net/11343/211688
Chicago Manual of Style (16th Edition):
Hamilton, Amy Louise. “Immunological and microbiological studies in post-operative Crohn's disease.” 2017. Doctoral Dissertation, University of Melbourne. Accessed March 08, 2021.
http://hdl.handle.net/11343/211688.
MLA Handbook (7th Edition):
Hamilton, Amy Louise. “Immunological and microbiological studies in post-operative Crohn's disease.” 2017. Web. 08 Mar 2021.
Vancouver:
Hamilton AL. Immunological and microbiological studies in post-operative Crohn's disease. [Internet] [Doctoral dissertation]. University of Melbourne; 2017. [cited 2021 Mar 08].
Available from: http://hdl.handle.net/11343/211688.
Council of Science Editors:
Hamilton AL. Immunological and microbiological studies in post-operative Crohn's disease. [Doctoral Dissertation]. University of Melbourne; 2017. Available from: http://hdl.handle.net/11343/211688
University of Hong Kong
30.
Teoh Chan, Ching-haan.
A serological study of
Pseudomonas aeruginosa with its relation to hospital
infection.
Degree: 1967, University of Hong Kong
URL: http://hdl.handle.net/10722/39527
Subjects/Keywords: Pseudomonas aeruginosa.;
Serology.;
Infection.
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to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Teoh Chan, C. (1967). A serological study of
Pseudomonas aeruginosa with its relation to hospital
infection. (Thesis). University of Hong Kong. Retrieved from http://hdl.handle.net/10722/39527
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Teoh Chan, Ching-haan. “A serological study of
Pseudomonas aeruginosa with its relation to hospital
infection.” 1967. Thesis, University of Hong Kong. Accessed March 08, 2021.
http://hdl.handle.net/10722/39527.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Teoh Chan, Ching-haan. “A serological study of
Pseudomonas aeruginosa with its relation to hospital
infection.” 1967. Web. 08 Mar 2021.
Vancouver:
Teoh Chan C. A serological study of
Pseudomonas aeruginosa with its relation to hospital
infection. [Internet] [Thesis]. University of Hong Kong; 1967. [cited 2021 Mar 08].
Available from: http://hdl.handle.net/10722/39527.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Teoh Chan C. A serological study of
Pseudomonas aeruginosa with its relation to hospital
infection. [Thesis]. University of Hong Kong; 1967. Available from: http://hdl.handle.net/10722/39527
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
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Open Access Theses and Dissertations
