You searched for subject:(Self assembled monolayers).
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1.
Wei, Xiaoliang.
Robust, Physisorbed Self-Assembled Monolayers on Graphite
and Their Applications in Nanoscience.
Degree: PhD, Chemistry, 2009, Brown University
URL: https://repository.library.brown.edu/studio/item/bdr:217/ 
► This dissertation work focuses on preparations and applications of robust, physisorbed SAMs on HOPG. The molecules contain an anthracene derivative core and two -CH2OC6H12OC22H45 side…
(more)
▼ This dissertation work focuses on preparations and
applications of robust, physisorbed SAMs on HOPG. The molecules
contain an anthracene derivative core and two -CH2OC6H12OC22H45
side chains. Robustness of the SAMs is examined through solvent
rinse experiments. Cold tetradecane (6°C), EtOH and pentane afford
"normal" SAMs, and are used to expose the SAMs. Two applications of
robust SAMs are investigated. The first is templated AuNP capture
and patterning. Acquisition of patterned AuNPs depends on strong
SAM ? HOPG and SAM ? AuNP interactions. Three strategies adopting
different SAM ? AuNP interactions are explored: (i) direct
place-exchange on DMAP-AuNPs using carboxylic acid SAMs; (ii) Cu2+
ion bridges using carboxylic acid-functionalized AuNPs and SAMs;
and (iii) Grubbs coupling between norbornene-functionalized AuNPs
and SAMs. Factors affecting AuNP capture and patterning are
investigated, including AuNP concentration, incubation time,
surface density of gold-binding functional groups in SAMs, and / or
number of SAM-binding functional groups on AuNPs. The Cu2+ bridge
strategy offers the best control over AuNP capture and patterning.
However, AuNP surface aggregates are commonly observed in all three
strategies, indicating the controls are limited. Surface
polymerization adds inter-molecular covalent bonds within
pre-formed SAMs, affording higher SAMs' physisorptions to HOPG. The
SAM Molecules containing two isothiocyanates are cross-linked
through reactions with 1,4-diaminobutane. The surface reaction
products are characterized by MALDI-TOF MS. Optimization of
reaction conditions using n-butylamine establishes 20mM diamine and
2 hours are suitable for surface polymerization. The surface
polymerization produces the monomer, dimer and trimer of the
diisothiocyanate. The time dependence of surface polymerization is
measured. Molecular conformer rectifiers (MCRs) form robust SAMs on
HOPG. Rectification that originates from bias-induced molecular
conformational changes are investigated using STM and STS. STM
imaging indicates that introduction of a "chemical marker" such as
S may be crucial to measure the rectification in mixed SAMs. STS
studies provide encouraging results, showing remarkable asymmetry
in i-V curves. However, the i-V curves are not constant due to
thermal drift.
Advisors/Committee Members: Zimmt, Matthew (director), Basu, Amit (reader), Williard, Paul (reader), Zimmt, Matthew (director), Basu, Amit (reader), Williard, Paul (reader).
Subjects/Keywords: self-assembled monolayers
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APA (6th Edition):
Wei, X. (2009). Robust, Physisorbed Self-Assembled Monolayers on Graphite
and Their Applications in Nanoscience. (Doctoral Dissertation). Brown University. Retrieved from https://repository.library.brown.edu/studio/item/bdr:217/
Chicago Manual of Style (16th Edition):
Wei, Xiaoliang. “Robust, Physisorbed Self-Assembled Monolayers on Graphite
and Their Applications in Nanoscience.” 2009. Doctoral Dissertation, Brown University. Accessed January 17, 2021.
https://repository.library.brown.edu/studio/item/bdr:217/.
MLA Handbook (7th Edition):
Wei, Xiaoliang. “Robust, Physisorbed Self-Assembled Monolayers on Graphite
and Their Applications in Nanoscience.” 2009. Web. 17 Jan 2021.
Vancouver:
Wei X. Robust, Physisorbed Self-Assembled Monolayers on Graphite
and Their Applications in Nanoscience. [Internet] [Doctoral dissertation]. Brown University; 2009. [cited 2021 Jan 17].
Available from: https://repository.library.brown.edu/studio/item/bdr:217/.
Council of Science Editors:
Wei X. Robust, Physisorbed Self-Assembled Monolayers on Graphite
and Their Applications in Nanoscience. [Doctoral Dissertation]. Brown University; 2009. Available from: https://repository.library.brown.edu/studio/item/bdr:217/
Vanderbilt University
2.
Tedjo, Chrysanty.
Controlling protein-surface interactions in chromatography using mixed self-assembled monolayers.
Degree: PhD, Chemical Engineering, 2011, Vanderbilt University
URL: http://hdl.handle.net/1803/11289
► High performance liquid chromatography (HPLC) has become an essential tool in biotechnology. Numerous proteins have been separated by HPLC for analytical and preparative separation purposes.…
(more)
▼ High performance liquid chromatography (HPLC) has become an essential tool in biotechnology. Numerous proteins have been separated by HPLC for analytical and preparative separation purposes. The main challenge in protein chromatography is the ability to isolate the protein of interest in its active form with high purity. The goal of this research is to provide a generic chromatographic approach for separating proteins efficiently with high retention of their biological activity. Here, I utilized mixed
self self-
assembled monolayers (SAMs) of Cl3Si(CH2)11(OCH2CH2)3OCH3 (EG3OMe) and n-octyltrichlorosilane (C8) to modulate protein-silica support interactions in a controlled manner. The oligo(ethylene glycol) tail groups in the EG3OMe SAM display a surface hydrophilicity that minimizes interactions with proteins. The C8 SAM displays a hydrophobic surface that adsorbs proteins. With mixed SAMs, the wetting properties of their surface and their level of interactions with proteins can be controlled.
Mixed SAMs of EG3OMe and C8 were deposited on SiO2/Si substrates and silica supports. Analysis using x-ray photoelectron spectroscopy showed that the surface compositions of mixed SAM-coated particles were similar to those of mixed SAMs formed on flat substrates. The critical surface tensions of the mixed SAM-coated silica particles, estimated by a developed flotation method, were similar to those of the same mixed SAMs on SiO2/Si substrates, as measured using contact angle goniometry.
The performances of these mixed SAM coated-supports were examined in a HPLC system using several model proteins. The results from chromatographic experiments showed that protein retention and separation in a chromatographic column was controllable by selecting the appropriate level of column hydrophobicity and the concentration of salt in the mobile phase. Analysis of protein mass recoveries after chromatography revealed that the rate loss of protein in the column was primarily a function of the protein residence time.
The described methods provide a systematic way for controlling protein retention during chromatography by tuning the hydrophobicity of the support. With proper selection, a surface hydrophobicity can be produced so that a protein can be effectively retained by a support without resulting in its permanent loss during chromatography.
Advisors/Committee Members: G. Kane Jennings (committee member), M. Douglas LeVan (committee member), Scott A. Guelcher (committee member), David E. Cliffel (committee member), Paul E. Laibinis (Committee Chair).
Subjects/Keywords: self-assembled monolayers; chromatography; protein separation
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APA (6th Edition):
Tedjo, C. (2011). Controlling protein-surface interactions in chromatography using mixed self-assembled monolayers. (Doctoral Dissertation). Vanderbilt University. Retrieved from http://hdl.handle.net/1803/11289
Chicago Manual of Style (16th Edition):
Tedjo, Chrysanty. “Controlling protein-surface interactions in chromatography using mixed self-assembled monolayers.” 2011. Doctoral Dissertation, Vanderbilt University. Accessed January 17, 2021.
http://hdl.handle.net/1803/11289.
MLA Handbook (7th Edition):
Tedjo, Chrysanty. “Controlling protein-surface interactions in chromatography using mixed self-assembled monolayers.” 2011. Web. 17 Jan 2021.
Vancouver:
Tedjo C. Controlling protein-surface interactions in chromatography using mixed self-assembled monolayers. [Internet] [Doctoral dissertation]. Vanderbilt University; 2011. [cited 2021 Jan 17].
Available from: http://hdl.handle.net/1803/11289.
Council of Science Editors:
Tedjo C. Controlling protein-surface interactions in chromatography using mixed self-assembled monolayers. [Doctoral Dissertation]. Vanderbilt University; 2011. Available from: http://hdl.handle.net/1803/11289
Duquesne University
3.
Kruszewski, Kristen Marie.
Inhibiting Bacterial Biofilm Formation on Stainless Steel 316L Using Self-Assembled Monolayers.
Degree: PhD, Chemistry and Biochemistry, 2012, Duquesne University
URL: https://dsc.duq.edu/etd/1524
► Stainless steel 316L (SS316L) is commonly used to construct orthopedic implants, which can fail due to bacterial biofilm infection. Since infection typically occurs around the…
(more)
▼ Stainless steel 316L (SS316L) is commonly used to construct orthopedic implants, which can fail due to bacterial biofilm infection. Since infection typically occurs around the time of implant surgery, it is important to reduce bacterial adhesion early on during the wound healing process. Two approaches were utilized to combat the problem of bacterial adhesion using
self-
assembled monolayers (SAMs) as the basis for the surface modification. In a passive approach, SAMs with long alkyl chains presenting hydrophobic (-CH3) or hydrophilic (tri- and penta-ethylene glycol) tail groups were used. In an orthogonal approach, active antimicrobial coatings were formed by using SAMs to immobilize the antibiotics gentamicin or vancomycin individually and in combination. Modified surfaces were characterized using diffuse reflectance infrared Fourier transform (DRIFT) spectroscopy, matrix assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometry, atomic force microscopy (AFM), and contact angle analysis. Staphylococcus aureus biofilm growth on modified surfaces was monitored using confocal laser scanning microscopy (CLSM), scanning electron microscopy (SEM), and colony forming unit (CFU) analysis. Neither hydrophobic nor hydrophilic SAMs inhibited biofilm development. However, antibiotic-linked films significantly reduced biofilm growth by 99 % up to 48 hours. Gentamicin-linked films were shown to be effective from 2-24 hours while vancomycin-linked films significantly inhibited biofilm growth at longer time points (6-48 hours). Combining the antibiotics limited biofilm development from 2-24 hours. This is significant because reducing initial bacterial adhesion at early time points is critical to limiting biofilm infection and implant failure. Further, this approach would deliver active antibiotic molecules directly to the implant site and improve upon current treatment strategies which rely on high doses of intravenous antibiotics that can contribute to antibiotic resistance and increasingly virulent strains of bacteria.
Advisors/Committee Members: Ellen Gawalt, Rita Mihailescu, Jeffry Madura, Luanne Hall-Stoodley, Ralph Wheeler, David Seybert.
Subjects/Keywords: Biofilms; Orthopedic implants; Self-assembled monolayers
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APA (6th Edition):
Kruszewski, K. M. (2012). Inhibiting Bacterial Biofilm Formation on Stainless Steel 316L Using Self-Assembled Monolayers. (Doctoral Dissertation). Duquesne University. Retrieved from https://dsc.duq.edu/etd/1524
Chicago Manual of Style (16th Edition):
Kruszewski, Kristen Marie. “Inhibiting Bacterial Biofilm Formation on Stainless Steel 316L Using Self-Assembled Monolayers.” 2012. Doctoral Dissertation, Duquesne University. Accessed January 17, 2021.
https://dsc.duq.edu/etd/1524.
MLA Handbook (7th Edition):
Kruszewski, Kristen Marie. “Inhibiting Bacterial Biofilm Formation on Stainless Steel 316L Using Self-Assembled Monolayers.” 2012. Web. 17 Jan 2021.
Vancouver:
Kruszewski KM. Inhibiting Bacterial Biofilm Formation on Stainless Steel 316L Using Self-Assembled Monolayers. [Internet] [Doctoral dissertation]. Duquesne University; 2012. [cited 2021 Jan 17].
Available from: https://dsc.duq.edu/etd/1524.
Council of Science Editors:
Kruszewski KM. Inhibiting Bacterial Biofilm Formation on Stainless Steel 316L Using Self-Assembled Monolayers. [Doctoral Dissertation]. Duquesne University; 2012. Available from: https://dsc.duq.edu/etd/1524
University of Sydney
4.
Panduwinata, Dwi.
Scanning Tunneling Microscopy Investigation of Porphyrin Self-Assembled Monolayers at Solid/Liquid Interface
.
Degree: 2013, University of Sydney
URL: http://hdl.handle.net/2123/10012
► In this thesis the author presents Scanning Tunneling Microscopy (STM) study on the self-assembly of tetra-alkyl porphyrin (TAP) series at solid/liquid interface of highly-ordered pyrolytic…
(more)
▼ In this thesis the author presents Scanning Tunneling Microscopy (STM) study on the self-assembly of tetra-alkyl porphyrin (TAP) series at solid/liquid interface of highly-ordered pyrolytic graphite (HOPG) and 1-phenyloctane. This study determines whether altering the alkyl chain lengths and porphyrin concentrations in solution affects the molecular conformation of the SAMs. It also investigates whether the presence of axial chloride ligand on cobalt porphyrin metal centre can be detected using the STM technique. Porphyrins with long-alkyl chain lengths (i.e. C19-TAP, C17-TAP, and C15-TAP) form only one type of polymorph on HOPG. Shorter chain length porphyrins (C13-TAP and Co(II)C13-TAP) form two different polymorphs with the low- and high-density structural conformation on HOPG. At low concentrations (≤ 10-6 M) they form only the low-density polymorph. Only high-density polymorph is observed at high concentrations (≥ 10-3 M). The structural transformation between these polymorphs could be explained based on the different orientations of alkyl- chains that conserve the b lattice vector in both polymorphs. The preliminary study shows that porphyrin species with axial chloride ligand appears at lower apparent heights on STM measurements. This could be attributed to the electronic effect associated with the different cobalt oxidation states which is stabilised by the presence of chloride ligand. The study presented in this thesis demonstrates that structural modifications and concentration provide powerful approaches to tailor porphyrin monolayer with specific conformation on the surface. This study is crucial in the effort to fully realise the potentials of molecular self-assembly for tailoring porphyrin monolayers with specific functions and properties.
Subjects/Keywords: Scanning Tunneling Microscopy;
Porphyrin;
Self-Assembled Monolayers
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APA (6th Edition):
Panduwinata, D. (2013). Scanning Tunneling Microscopy Investigation of Porphyrin Self-Assembled Monolayers at Solid/Liquid Interface
. (Thesis). University of Sydney. Retrieved from http://hdl.handle.net/2123/10012
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Panduwinata, Dwi. “Scanning Tunneling Microscopy Investigation of Porphyrin Self-Assembled Monolayers at Solid/Liquid Interface
.” 2013. Thesis, University of Sydney. Accessed January 17, 2021.
http://hdl.handle.net/2123/10012.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Panduwinata, Dwi. “Scanning Tunneling Microscopy Investigation of Porphyrin Self-Assembled Monolayers at Solid/Liquid Interface
.” 2013. Web. 17 Jan 2021.
Vancouver:
Panduwinata D. Scanning Tunneling Microscopy Investigation of Porphyrin Self-Assembled Monolayers at Solid/Liquid Interface
. [Internet] [Thesis]. University of Sydney; 2013. [cited 2021 Jan 17].
Available from: http://hdl.handle.net/2123/10012.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Panduwinata D. Scanning Tunneling Microscopy Investigation of Porphyrin Self-Assembled Monolayers at Solid/Liquid Interface
. [Thesis]. University of Sydney; 2013. Available from: http://hdl.handle.net/2123/10012
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
NSYSU
5.
Huang, Chien-lin.
Study of Molecular Self-Assembled Monolayers of Ru(II)-Terpyridyloctanethiolate Complex on Au Electrode and Au Clusters.
Degree: Master, Chemistry, 2006, NSYSU
URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0717106-144532
► The cyclic voltammogram of complex 6 shows one successive reversible one-electron redox wave corresponding to the oxidation of the Ruthenium moiety and peak-to-peak separations are…
(more)
▼ The cyclic voltammogram of complex 6 shows one successive reversible one-electron redox wave corresponding to the oxidation of the Ruthenium moiety and peak-to-peak separations are smaller than 59 mV(ideal value of one electron transfer with diffusing controlling). In addition, the peak currents are linear to scan rate, i.e., i α V. This observation is corresponding to the electrochemical property of SAM, and we would like to suggest that the electron transfer process in the electrochemical measurements is direct controlling.
Furthermore, we synthesized a nano-material by using of redox stable Ru(II)-Terpyridyloctanethiol attached to gold cluster (complex 7). The clusters are stable in air, soluble in nonpolar organic solvents and the characters could be examining by traditional chemical instruments such as NMR, UV/Vis, TEM.
Finally, complex 7 seif-
assembled on gold electrode (complex 8). This observation is corresponding to the electrochemical property of SAM, and we would like to suggest that the electron transfer process in the electrochemical measurements is direct controlling. we would like to suggest that the complex 5 has bi-functionalized property.
Advisors/Committee Members: none (chair), Teng-Yuan Dong (committee member), none (chair).
Subjects/Keywords: molecular self-assembled monolayers; MPCs
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APA (6th Edition):
Huang, C. (2006). Study of Molecular Self-Assembled Monolayers of Ru(II)-Terpyridyloctanethiolate Complex on Au Electrode and Au Clusters. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0717106-144532
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Huang, Chien-lin. “Study of Molecular Self-Assembled Monolayers of Ru(II)-Terpyridyloctanethiolate Complex on Au Electrode and Au Clusters.” 2006. Thesis, NSYSU. Accessed January 17, 2021.
http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0717106-144532.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Huang, Chien-lin. “Study of Molecular Self-Assembled Monolayers of Ru(II)-Terpyridyloctanethiolate Complex on Au Electrode and Au Clusters.” 2006. Web. 17 Jan 2021.
Vancouver:
Huang C. Study of Molecular Self-Assembled Monolayers of Ru(II)-Terpyridyloctanethiolate Complex on Au Electrode and Au Clusters. [Internet] [Thesis]. NSYSU; 2006. [cited 2021 Jan 17].
Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0717106-144532.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Huang C. Study of Molecular Self-Assembled Monolayers of Ru(II)-Terpyridyloctanethiolate Complex on Au Electrode and Au Clusters. [Thesis]. NSYSU; 2006. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0717106-144532
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
6.
Liu, Chao.
The Application of the Self-Assembled Monolayers for Improving the Bioactivity of Biomaterials : 生体材料の生体活性向上に向けた自己組織化膜の応用.
Degree: 博士（工学）, 2017, Kyushu Institute of Technology / 九州工業大学
URL: http://hdl.handle.net/10228/5715
► 九州工業大学博士学位論文 学位記番号:生工博甲第256号 学位授与年月日:平成28年3月25日
GENERAL INTRODUCTION|2:THE STUDY OF 10-CARBOXYDECYLPHOSPHONIC ACID (10-CDPA) SELF-ASSEMBLED MONOLAYERS (SAMS) IN INDUCING APATITE FORMATION ON COBALT-CHROMIUM (Co-Cr) ALLOY|3:THE STUDY OF 11-AMINOUNDECYLPHOSPHONIC ACID…
(more)
▼ 九州工業大学博士学位論文 学位記番号:生工博甲第256号 学位授与年月日:平成28年3月25日
GENERAL INTRODUCTION|2:THE STUDY OF 10-CARBOXYDECYLPHOSPHONIC ACID (10-CDPA) SELF-ASSEMBLED MONOLAYERS (SAMS) IN INDUCING APATITE FORMATION ON COBALT-CHROMIUM (Co-Cr) ALLOY|3:THE STUDY OF 11-AMINOUNDECYLPHOSPHONIC ACID (11-AUPA) SELF‐ASSEMBLED MONOLAYERS (SAMS) AND POLY-γ-GLUTAMIC ACID (PGA) IN INDUCING APATITE FORMATION ON COBALT-CHROMIUM (Co-Cr) ALLOY|4:THE INFLUENCE OF NaOH PRETREATMENT ON INDUCING APATITE FORMATION ON 11-AUPA-MODIFIED COBALT-CHROMIUM (Co-Cr) ALLOYS IN THE SIMULATED BODY FLUID|5:THE STUDY OF 11-AUPA SELF-ASSEMBLED MONOLAYERS (SAMS) IN INDUCING APATITE FORMATION ON ALUMINA CERAMIC|6:GENERAL CONCLUSION
平成27年度
Advisors/Committee Members: 宮崎, 敏樹.
Subjects/Keywords: Self-assembled monolayers; Simulated body fluid(SBF); Apatite; Biomaterials; Bioactivity
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APA (6th Edition):
Liu, C. (2017). The Application of the Self-Assembled Monolayers for Improving the Bioactivity of Biomaterials : 生体材料の生体活性向上に向けた自己組織化膜の応用. (Thesis). Kyushu Institute of Technology / 九州工業大学. Retrieved from http://hdl.handle.net/10228/5715
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Liu, Chao. “The Application of the Self-Assembled Monolayers for Improving the Bioactivity of Biomaterials : 生体材料の生体活性向上に向けた自己組織化膜の応用.” 2017. Thesis, Kyushu Institute of Technology / 九州工業大学. Accessed January 17, 2021.
http://hdl.handle.net/10228/5715.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Liu, Chao. “The Application of the Self-Assembled Monolayers for Improving the Bioactivity of Biomaterials : 生体材料の生体活性向上に向けた自己組織化膜の応用.” 2017. Web. 17 Jan 2021.
Vancouver:
Liu C. The Application of the Self-Assembled Monolayers for Improving the Bioactivity of Biomaterials : 生体材料の生体活性向上に向けた自己組織化膜の応用. [Internet] [Thesis]. Kyushu Institute of Technology / 九州工業大学; 2017. [cited 2021 Jan 17].
Available from: http://hdl.handle.net/10228/5715.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Liu C. The Application of the Self-Assembled Monolayers for Improving the Bioactivity of Biomaterials : 生体材料の生体活性向上に向けた自己組織化膜の応用. [Thesis]. Kyushu Institute of Technology / 九州工業大学; 2017. Available from: http://hdl.handle.net/10228/5715
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Texas A&M University
7.
Mahadevan, Aishwarya.
Comparison of Molecular-Wires for Enhancing Charge Transport of Enzymatic Electrode Assemblies: A Glycerol Bioanode Model.
Degree: MS, Biological and Agricultural Engineering, 2014, Texas A&M University
URL: http://hdl.handle.net/1969.1/154062
► Biomolecules are inherently less conductive. Therefore, bio-electronic devices that depend on conventional biomolecules to tether enzymes onto electrode supports and to shuttle electrons between the…
(more)
▼ Biomolecules are inherently less conductive. Therefore, bio-electronic devices that depend on conventional biomolecules to tether enzymes onto electrode supports and to shuttle electrons between the enzyme and the electrode suffer from charge dissipation. This results in bioanondes with decreased current-voltage responses as a result of ohmic losses. Reducing the internal resistance is the simplest way of increasing the current-voltage response associated with bioanodes and has yet been an unmet challenge. A novel iron (II) sulfide (FeS) based molecular wiring system was developed for immobilizing glycerol dehydrogenase on a gold electrode surface. Amperometric and potentiometric analyses with glycerol dehydrogenase-based model electrodes confirmed the ability of this single-molecule to remarkably amplify, about ten-fold increase in current and up to 24% increase in voltage outputs, as compared to electrodes fabricated with the conventional Pyrroloquinoline quinone-based composite molecular wiring system. FeS achieves the dual purpose of anchoring the enzyme to the gold electrode while also mediating electron shuttling between coenzyme and the electrode surface. This dual functionality allows usage of a single-molecular wire to foster electrical communication between the enzyme and the electrode instead of the conventional multi-molecular wiring system and in turn reducing the internal resistance of the electrode. The resulting increase in current/voltage response opens up a wide range of possibilities for developing efficient bio-electrodes for bioelectronics applications.
Advisors/Committee Members: Fernando, Sandun (advisor), Karthikeyan, Raghupathy (committee member), McShane, Mike (committee member).
Subjects/Keywords: Bioanode; Enzyme immobilization; Glycerol dehydrogenase; Molecular wiring; Self-assembled monolayers
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APA ·
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APA (6th Edition):
Mahadevan, A. (2014). Comparison of Molecular-Wires for Enhancing Charge Transport of Enzymatic Electrode Assemblies: A Glycerol Bioanode Model. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/154062
Chicago Manual of Style (16th Edition):
Mahadevan, Aishwarya. “Comparison of Molecular-Wires for Enhancing Charge Transport of Enzymatic Electrode Assemblies: A Glycerol Bioanode Model.” 2014. Masters Thesis, Texas A&M University. Accessed January 17, 2021.
http://hdl.handle.net/1969.1/154062.
MLA Handbook (7th Edition):
Mahadevan, Aishwarya. “Comparison of Molecular-Wires for Enhancing Charge Transport of Enzymatic Electrode Assemblies: A Glycerol Bioanode Model.” 2014. Web. 17 Jan 2021.
Vancouver:
Mahadevan A. Comparison of Molecular-Wires for Enhancing Charge Transport of Enzymatic Electrode Assemblies: A Glycerol Bioanode Model. [Internet] [Masters thesis]. Texas A&M University; 2014. [cited 2021 Jan 17].
Available from: http://hdl.handle.net/1969.1/154062.
Council of Science Editors:
Mahadevan A. Comparison of Molecular-Wires for Enhancing Charge Transport of Enzymatic Electrode Assemblies: A Glycerol Bioanode Model. [Masters Thesis]. Texas A&M University; 2014. Available from: http://hdl.handle.net/1969.1/154062
Penn State University
8.
Calkins, Jacob Andrew.
FUNCTIONALIZED BIOINSPIRED MICROSTRUCTURED OPTICAL FIBER PORES
FOR APPLICATIONS IN CHEMICAL VAPOR SENSING
.
Degree: 2011, Penn State University
URL: https://submit-etda.libraries.psu.edu/catalog/11669
► Chemical vapor sensing for defense, homeland security, environmental, and agricultural application is a challenge, which due combined requirements of ppt sensitivity, high selectivity, and rapid…
(more)
▼ Chemical vapor sensing for defense, homeland security, environmental, and agricultural
application is a challenge, which due combined requirements of ppt sensitivity, high
selectivity, and rapid response, cannot be met using conventional analytical chemistry
techniques. New sensing approaches and platforms are necessary in order to make
progress in this rapidly evolving field. Inspired by the functionalized nanopores on moth
sensilla hairs that contribute to the high selectivity and sensitivity of this biological system,
a chemical vapor sensor based on the micro to nanoscale pores in microstructured optical
fibers (MOFs) was designed. This MOF based chemical vapor sensor design utilizes MOF
pores functionalized with organic
self‐
assembled monolayers (SAMs) for selectivity and
separations and a gold plasmonic sensor for detection and discrimination.
Thin well‐controlled gold films in MOF pores are critical components for the fabrication
of structured plasmonic chemical vapor sensors. Thermal decomposition of dimethyl
Au(II) trifluoroacetylacetonate dissolved in near‐critical CO2 was used to deposit gold
island films within the MOF pores. Using a 3‐mercatopropyltrimethoxysilane adhesion
layer, continuous gold thin films as thin as 20‐30 nm were deposited within MOF pores as
small as 500 nm in diameter. The gold island films proved to be SERS active and were used
to detect 900 ppt 2,4 DNT vapor in high pressure nitrogen and 6 ppm benzaldehyde.
MOF based waveguide Raman (WGR), which can probe the air/silica interface between a
waveguiding core and surrounding pores, was developed to detect and characterize SAMs
and other thin films deposited in micro to nanoscale MOF pores. MOF based WGR was used
to characterize an octadecyltrichlorosilane (OTS) SAM deposited in 1.6 μm diameter pores
iv
to demonstrate that the SAM was well‐formed, uniform along the pore length, and only a
single layer. MOF based WGR was used to detect a human serum albumin monolayer
deposited on the OTS SAM and monitor in‐situ the combustion of an OTS SAM in high
pressure oxygen.
Light scattering, an optical characterization technique that provides ellipsometric data
from micro to nanoscale cylinders, was developed in order to characterize highly smooth
wires and MOF pores. Clean, bare gold wires etched from MOF pore templates were found
to have angle dependent Ψ and Δ values that agree with numerically calculated and finite
element modeled values over the full 340o angular collection range. Light scattering was
shown to be sensitive to ellipticities in the cross‐section of silica, gold, and silicon wires
down to 1%. Using alkanethiol SAMs deposited on gold wires, light scattering was
demonstrated to be able to detect films as thin as 1.5 nm, and able to distinguish between a
decanethiol (1.5 nm) and an octadecanethiol SAM (2.7 mn). The high sensitivity of light
scattering will allow it to characterize SAMs and thin films on the inner surfaces of MOF
pores.
WGR and light scattering provide…
Advisors/Committee Members: John V Badding, Dissertation Advisor/Co-Advisor, John V Badding, Committee Chair/Co-Chair, James Bernhard Anderson, Committee Member, Christine Dolan Keating, Committee Member, Venkatraman Gopalan, Committee Member.
Subjects/Keywords: Light Scattering; Microstructured Optical Fibers; SERS; Waveguide Raman; Self-assembled Monolayers
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APA (6th Edition):
Calkins, J. A. (2011). FUNCTIONALIZED BIOINSPIRED MICROSTRUCTURED OPTICAL FIBER PORES
FOR APPLICATIONS IN CHEMICAL VAPOR SENSING
. (Thesis). Penn State University. Retrieved from https://submit-etda.libraries.psu.edu/catalog/11669
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Calkins, Jacob Andrew. “FUNCTIONALIZED BIOINSPIRED MICROSTRUCTURED OPTICAL FIBER PORES
FOR APPLICATIONS IN CHEMICAL VAPOR SENSING
.” 2011. Thesis, Penn State University. Accessed January 17, 2021.
https://submit-etda.libraries.psu.edu/catalog/11669.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Calkins, Jacob Andrew. “FUNCTIONALIZED BIOINSPIRED MICROSTRUCTURED OPTICAL FIBER PORES
FOR APPLICATIONS IN CHEMICAL VAPOR SENSING
.” 2011. Web. 17 Jan 2021.
Vancouver:
Calkins JA. FUNCTIONALIZED BIOINSPIRED MICROSTRUCTURED OPTICAL FIBER PORES
FOR APPLICATIONS IN CHEMICAL VAPOR SENSING
. [Internet] [Thesis]. Penn State University; 2011. [cited 2021 Jan 17].
Available from: https://submit-etda.libraries.psu.edu/catalog/11669.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Calkins JA. FUNCTIONALIZED BIOINSPIRED MICROSTRUCTURED OPTICAL FIBER PORES
FOR APPLICATIONS IN CHEMICAL VAPOR SENSING
. [Thesis]. Penn State University; 2011. Available from: https://submit-etda.libraries.psu.edu/catalog/11669
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Penn State University
9.
Bauer, James William.
Influence of Surface Composition and Wettability upon Contact Activation of Blood Plasma
.
Degree: 2011, Penn State University
URL: https://submit-etda.libraries.psu.edu/catalog/12052
► Coagulation resulting from contact activation due to blood-material interactions remains a challenge in the use of blood-contacting medical devices. Our group is investigating the protein-surface…
(more)
▼ Coagulation resulting from contact activation due to blood-material interactions remains a
challenge in the use of blood-contacting medical devices. Our group is investigating the
protein-surface interactions influencing material-induced blood plasma coagulation with the goal of contributing towards the rational development of biomaterials with improved
hemocompatability. Traditional biochemical theory of contact activation imparts specific
activating abilities to negatively-charged hydrophilic surface. However, recent studies have
indicated a need to revise this view as activation of blood factor XII (FXII), a contact
activation protein, is not specific to negatively-charged surfaces. The central hypothesis of this dissertation proposes that all material surfaces are potential activators of the intrinsic coagulation cascade, but protein adsorption moderates the molecular interactions of contact activation. Measurements of FXII activation in buffer solutions in the presence of mixed thiol
self-
assembled monolayers suggest a minimum of FXII activation for surfaces with wettabilities nearing 45 dyne/cm. From direct measurement of kallikrein activation at test surfaces we observed that contact activation reactions involving FXII, high-molecular weight kininogen, and prekallikrein are not specific to negatively-charged hydrophilic surfaces, lending further support for the role of protein adsorption-competition at procoagulant surfaces. Preliminary investigations into contact activation of blood plasma coagulation
patterned thiol-modified surfaces suggest spatial chemical heterogeneity influences blood
plasma coagulation. In summary, the studies described in this dissertation underscore the
need to view all materials as potential activators of the intrinsic coagulation cascade with protein adsorption moderating interactions of contact activation proteins.
Advisors/Committee Members: Christopher Alan Siedlecki, Dissertation Advisor/Co-Advisor, Christopher Alan Siedlecki, Committee Chair/Co-Chair, Erwin A Vogler, Committee Chair/Co-Chair, William Joseph Weiss, Committee Member, Ira Joseph Ropson, Committee Member, Alan J Snyder, Committee Member.
Subjects/Keywords: blood plasma coagulation; contact activation; factor XII; self assembled monolayers
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APA (6th Edition):
Bauer, J. W. (2011). Influence of Surface Composition and Wettability upon Contact Activation of Blood Plasma
. (Thesis). Penn State University. Retrieved from https://submit-etda.libraries.psu.edu/catalog/12052
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Bauer, James William. “Influence of Surface Composition and Wettability upon Contact Activation of Blood Plasma
.” 2011. Thesis, Penn State University. Accessed January 17, 2021.
https://submit-etda.libraries.psu.edu/catalog/12052.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Bauer, James William. “Influence of Surface Composition and Wettability upon Contact Activation of Blood Plasma
.” 2011. Web. 17 Jan 2021.
Vancouver:
Bauer JW. Influence of Surface Composition and Wettability upon Contact Activation of Blood Plasma
. [Internet] [Thesis]. Penn State University; 2011. [cited 2021 Jan 17].
Available from: https://submit-etda.libraries.psu.edu/catalog/12052.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Bauer JW. Influence of Surface Composition and Wettability upon Contact Activation of Blood Plasma
. [Thesis]. Penn State University; 2011. Available from: https://submit-etda.libraries.psu.edu/catalog/12052
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Houston
10.
Zenasni, Oussama.
Structural Modification of Partially Fluorinated Alkanethiols: Their Impact on the Properties of Fluorinated Self-Assembled Monolayers.
Degree: PhD, Chemistry, University of Houston
URL: http://hdl.handle.net/10657/3400
► The ability to fine-tune structural parameters for partially fluorinated alkanethiols opens avenues for the fabrication of fluorinated films with certain targeted physical properties. This dissertation…
(more)
▼ The ability to fine-tune structural parameters for partially fluorinated alkanethiols opens avenues for the fabrication of fluorinated films with certain targeted physical properties. This dissertation focuses on the modification of fluorinated alkanethiols in order to provide new types of fluorinated
self-
assembled monolayers (SAMs) as model films to study their physical properties. In one study, we developed a new fluorinated surfactant that bears a methyl terminal group; CH3(CF2)6(CH2)nSH (H1F6HnSH where n = 10, 11). This amphiphile created an unprecedented HC–FC dipole at the SAMs interface. When comparing the structural properties of SAMs of H1F6H11SH to those of octadecanethiol and 18,18,18-trifluorooctadecanethiol, films generated from H1F6H11SH were found to have a well-packed underlying hydrocarbon assembly. However, this property disrupts the orderliness of the perfluorinated segments, which gives rise to anomalies in the interfacial properties when comparing even and odd numbered chains.
To draw a deeper understanding of the role of the underlying fluorinated segment, we synthesized and generated
monolayers from alkyl-capped partially fluorinated alkanethiols; H(CH2)n(CF2)6(CH2)11SH (HnF6H11SH; where n = 3, 4, 5). These films revealed three important findings: 1) the HC–FC dipole ceases to affect the wetting behavior of the films as it is buried underneath the alkyl unit, 2) the orientation of the terminal methyl follows an odd-even trend as a function of the size of the upper alkyl segment and 3) a disordered interface is generated when extending the alkyl cap to five hydrocarbons.
A separate study involves the manipulation of the interfacial properties of SAMs by introducing three new surfactants that are highly fluorinated with a small propyl unit as an alkyl spacer: F(CF2)n(CH2)3SH, FnH3, where n = 8, 10, 12. An evaluation of the performance of the FnH3 series in relation to the known SAMs using ellipsometry, XPS, and wettability analysis, indicate that the newly fluorinated surfactants not only behave similar to those formed with the shorter ethyl spacer, but they also provide an improved packing structure. Such work expands the scope of molecules that generate films that are close to a completely perfluorinated surface, while improving the packing ability of the adsorbates.
Advisors/Committee Members: Lee, T. Randall (advisor), Baldelli, Steven (committee member), Cai, Chengzhi (committee member), Forrest, Rebecca L. (committee member), Miljanić, Ognjen Š. (committee member).
Subjects/Keywords: Fluorinated self-assembled monolayers (FSAMs)
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APA (6th Edition):
Zenasni, O. (n.d.). Structural Modification of Partially Fluorinated Alkanethiols: Their Impact on the Properties of Fluorinated Self-Assembled Monolayers. (Doctoral Dissertation). University of Houston. Retrieved from http://hdl.handle.net/10657/3400
Note: this citation may be lacking information needed for this citation format:
No year of publication.
Chicago Manual of Style (16th Edition):
Zenasni, Oussama. “Structural Modification of Partially Fluorinated Alkanethiols: Their Impact on the Properties of Fluorinated Self-Assembled Monolayers.” Doctoral Dissertation, University of Houston. Accessed January 17, 2021.
http://hdl.handle.net/10657/3400.
Note: this citation may be lacking information needed for this citation format:
No year of publication.
MLA Handbook (7th Edition):
Zenasni, Oussama. “Structural Modification of Partially Fluorinated Alkanethiols: Their Impact on the Properties of Fluorinated Self-Assembled Monolayers.” Web. 17 Jan 2021.
Note: this citation may be lacking information needed for this citation format:
No year of publication.
Vancouver:
Zenasni O. Structural Modification of Partially Fluorinated Alkanethiols: Their Impact on the Properties of Fluorinated Self-Assembled Monolayers. [Internet] [Doctoral dissertation]. University of Houston; [cited 2021 Jan 17].
Available from: http://hdl.handle.net/10657/3400.
Note: this citation may be lacking information needed for this citation format:
No year of publication.
Council of Science Editors:
Zenasni O. Structural Modification of Partially Fluorinated Alkanethiols: Their Impact on the Properties of Fluorinated Self-Assembled Monolayers. [Doctoral Dissertation]. University of Houston; Available from: http://hdl.handle.net/10657/3400
Note: this citation may be lacking information needed for this citation format:
No year of publication.
University of Houston
11.
Yang, Bin 1988-.
SELF-ASSEMBLED MONOLAYERS ON SI(111): FORMATION, CHARACTERIZATION, MODIFICATION, AND PROTEIN ADSORPTION.
Degree: MS, Chemistry, 2014, University of Houston
URL: http://hdl.handle.net/10657/1671
► The aim of this thesis was to design and develop a stepwise strategy for fabricating protein resistant silicon surfaces. The adsorbate molecule comprises two alkyne…
(more)
▼ The aim of this thesis was to design and develop a stepwise strategy for fabricating protein resistant silicon surfaces. The adsorbate molecule comprises two alkyne groups separated by an 8 carbon alkyl chain and a triethyleneglycol chain. This diyne molecule was able to form 24 Å thick
monolayers on hydrogen terminated Si(111) under high vacuum conditions via UV assisted hydrosilylation reaction. The estimated packing density of the
monolayers was 60%, indicating a very dense packing of the adsorbates. Thus closely packed
monolayers yielded a good stability both in ambient air and the PBS solution.
By screening CuAAC (Copper-Catalyzed Azide-Alkyne Cycloaddition) reaction conditions, it was found that with a reagent concentration of 1.25 mM copper, 10 mM OEG7-azide, 25 mM ascorbate, and 1.25 mM histidine as accelerating ligand, and a reaction time of 2 h in an anaerobic chamber, a modified film with minimum protein adsorbance could be formed. The non-basicity and non-toxicity of this commercially available ligand histidine is believed to have important applications in the field of bio-conjugation, and bio-adhesions.
An apparatus with the use of a multichannel pipette and multiwell plate, the methodology of optimization and the screening of more CuAAC reaction conditions on the surfaces was designed and built. Preliminary results showed that the fluorescence intensity data resulted from adsorbed fibrinogen-Alexa Flore 488 was reliably comparable with that of the traditional ellipsometry and XPS data, and under higher ligand concentrations, four similar compounds could be used as effective accelerating ligands for CuAAC reactions on the surfaces.
Advisors/Committee Members: Cai, Chengzhi (advisor), Do, Loi H. (committee member), Baldelli, Steven (committee member), Hu, Yandi (committee member).
Subjects/Keywords: Self-assembled monolayers (SAMs); Si(111); Click Reaction; Protein Adsorption
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APA (6th Edition):
Yang, B. 1. (2014). SELF-ASSEMBLED MONOLAYERS ON SI(111): FORMATION, CHARACTERIZATION, MODIFICATION, AND PROTEIN ADSORPTION. (Masters Thesis). University of Houston. Retrieved from http://hdl.handle.net/10657/1671
Chicago Manual of Style (16th Edition):
Yang, Bin 1988-. “SELF-ASSEMBLED MONOLAYERS ON SI(111): FORMATION, CHARACTERIZATION, MODIFICATION, AND PROTEIN ADSORPTION.” 2014. Masters Thesis, University of Houston. Accessed January 17, 2021.
http://hdl.handle.net/10657/1671.
MLA Handbook (7th Edition):
Yang, Bin 1988-. “SELF-ASSEMBLED MONOLAYERS ON SI(111): FORMATION, CHARACTERIZATION, MODIFICATION, AND PROTEIN ADSORPTION.” 2014. Web. 17 Jan 2021.
Vancouver:
Yang B1. SELF-ASSEMBLED MONOLAYERS ON SI(111): FORMATION, CHARACTERIZATION, MODIFICATION, AND PROTEIN ADSORPTION. [Internet] [Masters thesis]. University of Houston; 2014. [cited 2021 Jan 17].
Available from: http://hdl.handle.net/10657/1671.
Council of Science Editors:
Yang B1. SELF-ASSEMBLED MONOLAYERS ON SI(111): FORMATION, CHARACTERIZATION, MODIFICATION, AND PROTEIN ADSORPTION. [Masters Thesis]. University of Houston; 2014. Available from: http://hdl.handle.net/10657/1671
University of Houston
12.
Tarkington, Lynn 1983-.
Self-Assembled Monolayers Generated from Cholesterol-Based Thiols.
Degree: PhD, Chemistry, 2012, University of Houston
URL: http://hdl.handle.net/10657/1942
► Self-assembled monolayers (SAMs) generated from adsorption of the single-chained cholesterol-based and unsymmetrical double-chained cholesterol-based thiols on gold substrates were examined. The single-chained cholesterol-based thiols have…
(more)
▼ Self-
assembled monolayers (SAMs) generated from adsorption of the single-chained cholesterol-based and unsymmetrical double-chained cholesterol-based thiols on gold substrates were examined. The single-chained cholesterol-based thiols have a thiol headgroup, hydrocarbon spacer consisting of 3 – 12 methylene units, and a cholesterol tailgroup. The double-chained cholesterol-based thiols consist of a thiol headgroup and two tailgroups: an alkyl chain and a cholesterol-based moiety. Two series of single-component SAMs were formed from the cholesterol-based thiols. Analysis of the pure SAM shows that the added methylene spacer for the single-chained thiols allows for the formation of a better monolayer than that formed from thiocholesterol. The pure SAMs formed from the double-chained cholesterol-based thiols also produce a monolayer that is denser than the SAM generated from thiocholesterol, with interfacial properties resembling a SAM formed from normal alkanethiols. A series of binary SAMs generated from the single-chained cholesterol-based thiols and n-octadecanethiol exhibit properties of the cholesterol condensing effect. The SAMs were characterized using ellipsometry, contact angle goniometry, polarization modulation infrared reflection absorption spectroscopy (PM-IRRAS), and X-ray photoelectron spectroscopy (XPS). Overall, these cholesterol-based adsorbates were used to generate dense
monolayers despite a mixed composition of cholesterol moieties and trans-extended alkyl chains. The cholesterol condensing effect was observed in
self-
assembled monolayers for the first time.
Advisors/Committee Members: Lee, T. Randall (advisor), Gilbertson, Scott R. (committee member), Cai, Chengzhi (committee member), Hoffman, David M. (committee member), Forrest, Rebecca L. (committee member).
Subjects/Keywords: Cholesterol; Condensing Effect; Monolayer; Self-assembled monolayers (SAMs); Thiols
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APA (6th Edition):
Tarkington, L. 1. (2012). Self-Assembled Monolayers Generated from Cholesterol-Based Thiols. (Doctoral Dissertation). University of Houston. Retrieved from http://hdl.handle.net/10657/1942
Chicago Manual of Style (16th Edition):
Tarkington, Lynn 1983-. “Self-Assembled Monolayers Generated from Cholesterol-Based Thiols.” 2012. Doctoral Dissertation, University of Houston. Accessed January 17, 2021.
http://hdl.handle.net/10657/1942.
MLA Handbook (7th Edition):
Tarkington, Lynn 1983-. “Self-Assembled Monolayers Generated from Cholesterol-Based Thiols.” 2012. Web. 17 Jan 2021.
Vancouver:
Tarkington L1. Self-Assembled Monolayers Generated from Cholesterol-Based Thiols. [Internet] [Doctoral dissertation]. University of Houston; 2012. [cited 2021 Jan 17].
Available from: http://hdl.handle.net/10657/1942.
Council of Science Editors:
Tarkington L1. Self-Assembled Monolayers Generated from Cholesterol-Based Thiols. [Doctoral Dissertation]. University of Houston; 2012. Available from: http://hdl.handle.net/10657/1942
University of Manchester
13.
Castangia, Roberto.
Microarrays on gold : new applications for biocatalysis and proteomics.
Degree: PhD, 2012, University of Manchester
URL: https://www.research.manchester.ac.uk/portal/en/theses/microarrays-on-goldnew-applications-for-biocatalysis-and-proteomics(d384b464-2e40-409d-b104-6176ad7b6273).html
;
http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.559327
► Microarrays on gold have been used to develop new methodologies for biocatalysis and proteomic applications. The technology applies the logic of solid phase supported chemistry…
(more)
▼ Microarrays on gold have been used to develop new methodologies for biocatalysis and proteomic applications. The technology applies the logic of solid phase supported chemistry using self-assembled monolayers (SAMs) on a gold chip. The advantages of this technology are: i) an easy to handle platform, ii) parallel screening (64 reactions at once), iii) microliter scale reactions (1µL per sample), iv the use of mild conditions (buffers and t=37 °C), v) the absence of purification steps (only chip washing is required), vi) quick and accurate analysis by MALDI ToF MS.The metallopeptidase thermolysin was studied in peptide-coupling reactions to profile reactivity and specificity (Chapter 2). Reactivity was further investigated in transpeptidation reactions. Comparing the serine peptidase chymotrypsin with the zinc dependent thermolysin, it was found that transpeptidations proceed via N-transacylation reactions independent of a specific enzymatic catalytic mechanism (Chapter 3). These transacylations may be exploited for modifications of biocompatible and selective surfaces in ‘bottom-up’ bionanofabrication technologies. Selected peptidases with different catalytic mechanism were also arrayed to investigate polymerisation ability of dipeptides (Chapter 4). It was shown that oligomerisation can be obtained under mild conditions and a set of peptides was synthesised. Chapter 5 describes a new chemical methodology by which crude tryptic peptide digests can be trapped on chip and analysed by MALDI ToF MS without further purification steps. This dramatically improves time and cost efficiency.Finally, a new stepwise native chemical ligation methodology is proposed for amino acids, and peptides containing N-terminal cysteine residues (Chapter 6).
Subjects/Keywords: 553.4; Biocatalysis; Microarrays; Self-Assembled Monolayers; Peptide Synthesis; MALDI-ToF MS
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APA (6th Edition):
Castangia, R. (2012). Microarrays on gold : new applications for biocatalysis and proteomics. (Doctoral Dissertation). University of Manchester. Retrieved from https://www.research.manchester.ac.uk/portal/en/theses/microarrays-on-goldnew-applications-for-biocatalysis-and-proteomics(d384b464-2e40-409d-b104-6176ad7b6273).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.559327
Chicago Manual of Style (16th Edition):
Castangia, Roberto. “Microarrays on gold : new applications for biocatalysis and proteomics.” 2012. Doctoral Dissertation, University of Manchester. Accessed January 17, 2021.
https://www.research.manchester.ac.uk/portal/en/theses/microarrays-on-goldnew-applications-for-biocatalysis-and-proteomics(d384b464-2e40-409d-b104-6176ad7b6273).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.559327.
MLA Handbook (7th Edition):
Castangia, Roberto. “Microarrays on gold : new applications for biocatalysis and proteomics.” 2012. Web. 17 Jan 2021.
Vancouver:
Castangia R. Microarrays on gold : new applications for biocatalysis and proteomics. [Internet] [Doctoral dissertation]. University of Manchester; 2012. [cited 2021 Jan 17].
Available from: https://www.research.manchester.ac.uk/portal/en/theses/microarrays-on-goldnew-applications-for-biocatalysis-and-proteomics(d384b464-2e40-409d-b104-6176ad7b6273).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.559327.
Council of Science Editors:
Castangia R. Microarrays on gold : new applications for biocatalysis and proteomics. [Doctoral Dissertation]. University of Manchester; 2012. Available from: https://www.research.manchester.ac.uk/portal/en/theses/microarrays-on-goldnew-applications-for-biocatalysis-and-proteomics(d384b464-2e40-409d-b104-6176ad7b6273).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.559327
University of Edinburgh
14.
Piper, Andrew.
Electrochemical characterisation of microsquare nanoband edge electrode (MNEE) arrays and their use as biosensors.
Degree: PhD, 2017, University of Edinburgh
URL: http://hdl.handle.net/1842/28782
► Nanoelectrodes are defined as electrodes which have a critical dimension on the order of nanometres. Due to their smaller dimensions they have a reduced iR…
(more)
▼ Nanoelectrodes are defined as electrodes which have a critical dimension on the order of nanometres. Due to their smaller dimensions they have a reduced iR drop and enhanced mass transport, which results in the rapid establishment of an enhanced steady-state diffusion profile and a greater Faradaic current density, along with a smaller relative double layer capacitance, which together give a significantly increased signal to noise ratio compared to macroelectrodes. This potentially makes nanoelectrodes better sensors and analytical tools than macroelectrodes in terms of their having lower limits of detection and faster detection times. However, due to difficulties with fabrication most nanoelectrode designs are highly irreproducible which has inhibited their characterisation and commercial development. The Mount group has previously reported the design, fabrication and characterisation of a novel nanoelectrode design in conjunction with Engineers from the Scottish Microelectronic Centre (SMC). Microsquare Nanoband Edge Electrode arrays (MNEEs) consist of an array of cavities with nanoscale Pt bands (formed by sandwiching the metal between insulating layers) exposed around their perimeter. MNEEs are fabricated using a photolithographic process so can be reproducibly made in large quantities to high fidelity. The purpose of this work is to develop our understanding of the fundamental electrochemical behaviour of MNEEs for biosensing. First, a quantitative analysis of the cyclic voltammograms (CVs) and Electrochemical Impedance Spectroscopy (EIS) of macroelectrodes, microelectrodes and MNEE are compared and discussed. Second, their fundamental response is compared in terms of their biosensing properties by using a pre-established impedimetric biosensing protocol developed on macroelectrodes. This protocol uses a PNA probe to detect the mecA cassette of methicillin resistant staphylococcus aureus (MRSA). The procedure has been optimised and compared for macroelectrodes, microelectrodes and MNEE so as to compare their performances as biosensors. It was observed that MNEE’s: (a) form thiol films faster than electrodes with larger dimensions, determined by kinetic studies of 6-mercaptohexan-1-ol film formation (b) form films with different packing structures dependant on the electrode bulk to edge ratio (c) can detect the same concentration of target in less time than larger electrodes because of their increased sensitivity. The film packing has also been quantitatively investigated using EIS and it can be seen that films formed n MNEE were better able to incorporate target DNA into their more splayed out structure. Unique to this project has been the establishment of a protocol to form heterogeneous carbazole-alanine hydrogel matrices on nanoelectrodes, whose polymerisation is initiated by a pH swing at the electrode surface induced by the oxidation of hydroquinone. The gels growth pattern follows the diffusion field at the electrode and can be monitored using EIS. This also gives a measure of the permeability of the gel…
Subjects/Keywords: electrochemistry; biosensors; self-assembled monolayers; nanoelectrodes; hydrogels; electrochemical impedance spectroscopy; microfabrication
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APA (6th Edition):
Piper, A. (2017). Electrochemical characterisation of microsquare nanoband edge electrode (MNEE) arrays and their use as biosensors. (Doctoral Dissertation). University of Edinburgh. Retrieved from http://hdl.handle.net/1842/28782
Chicago Manual of Style (16th Edition):
Piper, Andrew. “Electrochemical characterisation of microsquare nanoband edge electrode (MNEE) arrays and their use as biosensors.” 2017. Doctoral Dissertation, University of Edinburgh. Accessed January 17, 2021.
http://hdl.handle.net/1842/28782.
MLA Handbook (7th Edition):
Piper, Andrew. “Electrochemical characterisation of microsquare nanoband edge electrode (MNEE) arrays and their use as biosensors.” 2017. Web. 17 Jan 2021.
Vancouver:
Piper A. Electrochemical characterisation of microsquare nanoband edge electrode (MNEE) arrays and their use as biosensors. [Internet] [Doctoral dissertation]. University of Edinburgh; 2017. [cited 2021 Jan 17].
Available from: http://hdl.handle.net/1842/28782.
Council of Science Editors:
Piper A. Electrochemical characterisation of microsquare nanoband edge electrode (MNEE) arrays and their use as biosensors. [Doctoral Dissertation]. University of Edinburgh; 2017. Available from: http://hdl.handle.net/1842/28782
15.
Cox, Jason R.
Controlling the Polymorphism of Active Pharmaceutical Ingredients with Two-Dimensional Templates.
Degree: MS, 2009, Worcester Polytechnic Institute
URL: etd-042709-112211
;
https://digitalcommons.wpi.edu/etd-theses/362
Self-assembled monolayers on gold and glass substrates are employed as templates to direct the crystal growth and polymorphism of active pharmaceutical ingredients. Orthogonal approaches are used to control polymorphism either through complementary hydrogen-bonding interactions or through repulsive interactions.
Advisors/Committee Members: Kristin K. Wobbe, Advisor, ;.Subjects/Keywords: Self-Assembled Monolayers; Polymorphism
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APA (6th Edition):
Cox, J. R. (2009). Controlling the Polymorphism of Active Pharmaceutical Ingredients with Two-Dimensional Templates. (Thesis). Worcester Polytechnic Institute. Retrieved from etd-042709-112211 ; https://digitalcommons.wpi.edu/etd-theses/362
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Cox, Jason R. “Controlling the Polymorphism of Active Pharmaceutical Ingredients with Two-Dimensional Templates.” 2009. Thesis, Worcester Polytechnic Institute. Accessed January 17, 2021.
etd-042709-112211 ; https://digitalcommons.wpi.edu/etd-theses/362.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Cox, Jason R. “Controlling the Polymorphism of Active Pharmaceutical Ingredients with Two-Dimensional Templates.” 2009. Web. 17 Jan 2021.
Vancouver:
Cox JR. Controlling the Polymorphism of Active Pharmaceutical Ingredients with Two-Dimensional Templates. [Internet] [Thesis]. Worcester Polytechnic Institute; 2009. [cited 2021 Jan 17].
Available from: etd-042709-112211 ; https://digitalcommons.wpi.edu/etd-theses/362.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Cox JR. Controlling the Polymorphism of Active Pharmaceutical Ingredients with Two-Dimensional Templates. [Thesis]. Worcester Polytechnic Institute; 2009. Available from: etd-042709-112211 ; https://digitalcommons.wpi.edu/etd-theses/362
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Waterloo
16.
Bedford, Erin.
Gold Surface Nanostructuring for Separation and Sensing of Biomolecules.
Degree: 2016, University of Waterloo
URL: http://hdl.handle.net/10012/11107
► Detecting biomolecules in physiological environments is critical to health care and environmental monitoring. In this work, we study and use gold surfaces for biomolecule detection…
(more)
▼ Detecting biomolecules in physiological environments is critical to health care and environmental monitoring. In this work, we study and use gold surfaces for biomolecule detection while incorporating nanoscale components—specifically, self-assembled monolayers (SAMs) of alkanethiols and gold nanostructured shells—with the goal of improving biomolecule detection methods. Using SAMs to functionalize gold surfaces can offer control over biomolecule binding density and orientation while still keeping the biomolecules near the sensing surface. Using surface IR spectroscopy, x-ray photoelectron spectroscopy (XPS), and density functional theory (DFT) modeling, we found that SAMs of short-chain and long-chain amine-terminated alkanethiols on gold had different sulphur binding environments. We also found that protein binding and recognition on the two different SAMs varied with SAM chain length and was also influenced by the presence of a cross-linker. In the second part of this work, we synthesized gold nanostructured shells on magnetic particles for combined separation and detection of biomolecules. We demonstrated their use as substrates for surface-enhanced Raman spectroscopy (SERS) As a proof-of-concept, we demonstrated the use of these particles to detect oligonucleotide binding and hybridization with SERS using a Raman-tagged oligonucleotide hairpin probe.
Subjects/Keywords: nanobiotechnology; nanoparticles; gold; self-assembled monolayers; biosensors; surface-enhanced Raman spectroscopy
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APA (6th Edition):
Bedford, E. (2016). Gold Surface Nanostructuring for Separation and Sensing of Biomolecules. (Thesis). University of Waterloo. Retrieved from http://hdl.handle.net/10012/11107
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Bedford, Erin. “Gold Surface Nanostructuring for Separation and Sensing of Biomolecules.” 2016. Thesis, University of Waterloo. Accessed January 17, 2021.
http://hdl.handle.net/10012/11107.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Bedford, Erin. “Gold Surface Nanostructuring for Separation and Sensing of Biomolecules.” 2016. Web. 17 Jan 2021.
Vancouver:
Bedford E. Gold Surface Nanostructuring for Separation and Sensing of Biomolecules. [Internet] [Thesis]. University of Waterloo; 2016. [cited 2021 Jan 17].
Available from: http://hdl.handle.net/10012/11107.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Bedford E. Gold Surface Nanostructuring for Separation and Sensing of Biomolecules. [Thesis]. University of Waterloo; 2016. Available from: http://hdl.handle.net/10012/11107
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Kentucky
17.
Safazadeh Haghighi, Leila.
DESIGN OF HIGHLY STABLE LOW-DENSITY SELF-ASSEMBLED MONOLAYERS USING THIOL-YNE CLICK REACTION FOR THE STUDY OF PROTEIN-SURFACE INTERACTIONS.
Degree: 2016, University of Kentucky
URL: https://uknowledge.uky.edu/cme_etds/61
► Protein adsorption on solid surfaces is a common yet complicated phenomenon that is not fully understood. Self-assembled monolayers have been utilized in many studies, as…
(more)
▼ Protein adsorption on solid surfaces is a common yet complicated phenomenon that is not fully understood. Self-assembled monolayers have been utilized in many studies, as well-defined model systems for studying protein-surface interactions in the atomic level. Various strategies, including the use of single component SAMs[1, 2], combinations of long and short alkanethiolates with methyl- and hydroxyl- terminal groups[3, 4], and using mixtures of alkanethiolates with similar chain length and varying terminal functional group [5] have been used to effectively control the surface wettability and determine the effect of surface composition and wettability on protein adsorption. In this dissertation we report key new findings on the effect of surface density of functional groups on protein adsorption phenomenon.
In The first phase of this research, we developed a novel approach for preparation of low-density self-assembled monolayers(LD-SAMs) on gold surfaces, based on radical-initiated thiol-yne click chemistry. This approach provides exceptional adsorbate stability and conformational freedom of interfacial functional groups, and is readily adapted for low-density monolayers of varied functionality. The resulting monolayers have two distinct phases: a highly crystalline head phase adjacent to the gold substrate, and a reduced density tail phase, which is in contact with the environment.
First, we investigated the feasibility of the proposed chemistry in solution-phase. In this approach, we synthesized “Y” shaped carboxylate-terminated thiol adsorbates via radical-initiated thiol-yne reaction. The LD-SAMs were then prepared through immersion of gold substrates into the solution of synthesized adsorbate molecules in hexane. The chemical structuring and electrochemical properties of resultant LD-SAMs were analyzed and compared with those of analogous traditional well-packed monolayers, using techniques such as Fourier transform infrared spectroscopy, ellipsometry, electrochemical impedance spectroscopy, reductive desorption, and contact angle goniometry. Characterization results indicated that resulting LD-SAMs have a lower average crystallinity, and higher electrochemical stability compared to well-packed monolayers. In addition, using a three-electrode system, we were able to show a reversible change in LD-SAM surface wettability, in response to an applied voltage. This remodeling capacity confirms the low density of the surface region of LD-SAM coatings.
The second area of work was focused on using the developed chemistry in solid-phase. The solid-phase approach minimized the required synthesis steps in solution-phase method, and used the photo-initiated thiol-yne click-reaction for grafting of acid-terminated alkynes to thiol-terminated monolayers on a gold substrate to create similar LD-SAMs as what were prepared through solution-phase process. We characterized the resulting monolayers and compared them to analogous well-packed SAMs and the also low-density monolayers prepared through the solution phase approach. The…
Subjects/Keywords: Self-Assembled Monolayers; Proteins; Thiol-Yne Click Reaction; Biomedical; Chemical Engineering
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APA (6th Edition):
Safazadeh Haghighi, L. (2016). DESIGN OF HIGHLY STABLE LOW-DENSITY SELF-ASSEMBLED MONOLAYERS USING THIOL-YNE CLICK REACTION FOR THE STUDY OF PROTEIN-SURFACE INTERACTIONS. (Doctoral Dissertation). University of Kentucky. Retrieved from https://uknowledge.uky.edu/cme_etds/61
Chicago Manual of Style (16th Edition):
Safazadeh Haghighi, Leila. “DESIGN OF HIGHLY STABLE LOW-DENSITY SELF-ASSEMBLED MONOLAYERS USING THIOL-YNE CLICK REACTION FOR THE STUDY OF PROTEIN-SURFACE INTERACTIONS.” 2016. Doctoral Dissertation, University of Kentucky. Accessed January 17, 2021.
https://uknowledge.uky.edu/cme_etds/61.
MLA Handbook (7th Edition):
Safazadeh Haghighi, Leila. “DESIGN OF HIGHLY STABLE LOW-DENSITY SELF-ASSEMBLED MONOLAYERS USING THIOL-YNE CLICK REACTION FOR THE STUDY OF PROTEIN-SURFACE INTERACTIONS.” 2016. Web. 17 Jan 2021.
Vancouver:
Safazadeh Haghighi L. DESIGN OF HIGHLY STABLE LOW-DENSITY SELF-ASSEMBLED MONOLAYERS USING THIOL-YNE CLICK REACTION FOR THE STUDY OF PROTEIN-SURFACE INTERACTIONS. [Internet] [Doctoral dissertation]. University of Kentucky; 2016. [cited 2021 Jan 17].
Available from: https://uknowledge.uky.edu/cme_etds/61.
Council of Science Editors:
Safazadeh Haghighi L. DESIGN OF HIGHLY STABLE LOW-DENSITY SELF-ASSEMBLED MONOLAYERS USING THIOL-YNE CLICK REACTION FOR THE STUDY OF PROTEIN-SURFACE INTERACTIONS. [Doctoral Dissertation]. University of Kentucky; 2016. Available from: https://uknowledge.uky.edu/cme_etds/61
Virginia Tech
18.
Qian, Chen.
Adsorption of Xyloglucan onto Cellulose and Cellulase onto Self-assembled Monolayers.
Degree: MS, Chemistry, 2012, Virginia Tech
URL: http://hdl.handle.net/10919/42496
► Adsorption of xyloglucan (XG) onto thin desulfated nanocrystalline cellulose (DNC) films was studied by surface plasmon resonance spectroscopy (SPR), quartz crystal microbalance with dissipation monitoring…
(more)
▼ Adsorption of xyloglucan (XG) onto thin desulfated nanocrystalline cellulose (DNC) films was studied by surface plasmon resonance spectroscopy (SPR), quartz crystal microbalance with dissipation monitoring (QCM-D), and atomic force microscopy (AFM) measurements. These studies were compared to adsorption studies of XG onto thin sulfated nanocrystalline cellulose (SNC) films and regenerated cellulose (RC) films performed by others. Collectively, these studies show the accessible surface area is the key factor for the differences in surface concentrations observed for XG adsorbed onto the three cellulose surfaces. XG penetrated into the porous nanocrystalline cellulose films. In contrast, XG was confined to the surfaces of the smooth, non-porous RC films. Surprisingly surface charge and cellulose morphology played a limited role on XG adsorption.
The effect of the non-ionic surfactant Tween 80 on the adsorption of cellulase onto alkane thiol
self-
assembled monolayers (SAMs) on gold was also studied. Methyl (-CH3), hydroxyl
(-OH) and carboxyl (-COOH) terminated SAMs were prepared. Adsorption of cellulase onto untreated and Tween 80-treated SAMs were monitored by SPR, QCM-D and AFM. The results indicated cellulase adsorption onto SAM-CH3 and SAM-COOH were driven by strong hydrophobic and electrostatic interactions, however, hydrogen bonding between cellulase and SAM-OH was weak. Tween 80 effectively hindered the adsorption of cellulase onto hydrophobic SAM-CH3 substrates. In contrast, it had almost no effect on the adsorption of cellulase onto SAM-OH and SAM-COOH substrates because of its reversible adsorption on these substrates.
Advisors/Committee Members: Roman, Maren (committeechair), Marand, Hervé L. (committee member), Esker, Alan R. (committeecochair).
Subjects/Keywords: cellulase; self-assembled monolayers; xyloglucan; cellulose thin films; adsorption
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APA (6th Edition):
Qian, C. (2012). Adsorption of Xyloglucan onto Cellulose and Cellulase onto Self-assembled Monolayers. (Masters Thesis). Virginia Tech. Retrieved from http://hdl.handle.net/10919/42496
Chicago Manual of Style (16th Edition):
Qian, Chen. “Adsorption of Xyloglucan onto Cellulose and Cellulase onto Self-assembled Monolayers.” 2012. Masters Thesis, Virginia Tech. Accessed January 17, 2021.
http://hdl.handle.net/10919/42496.
MLA Handbook (7th Edition):
Qian, Chen. “Adsorption of Xyloglucan onto Cellulose and Cellulase onto Self-assembled Monolayers.” 2012. Web. 17 Jan 2021.
Vancouver:
Qian C. Adsorption of Xyloglucan onto Cellulose and Cellulase onto Self-assembled Monolayers. [Internet] [Masters thesis]. Virginia Tech; 2012. [cited 2021 Jan 17].
Available from: http://hdl.handle.net/10919/42496.
Council of Science Editors:
Qian C. Adsorption of Xyloglucan onto Cellulose and Cellulase onto Self-assembled Monolayers. [Masters Thesis]. Virginia Tech; 2012. Available from: http://hdl.handle.net/10919/42496
Virginia Tech
19.
Zhang, Yafen.
Fundamental Studies of Reactions between NO3 Radicals and Organic Surfaces.
Degree: MS, Chemistry, 2012, Virginia Tech
URL: http://hdl.handle.net/10919/42372
► Ultrahigh vacuum (UHV) surface science experiments were designed to study reaction kinetics and mechanisms of gas-phase NO3 radicals with well-organized, highly characterized, organic thin films.…
(more)
▼ Ultrahigh vacuum (UHV) surface science experiments were designed to study reaction kinetics and mechanisms of gas-phase NO3 radicals with well-organized, highly characterized, organic thin films. The surface reactions were monitored in situ with reflection-absorption infrared spectroscopy (RAIRS). The oxidation states of
surface-bound molecules were identified with X-ray photoelectron spectroscopy (XPS). Consumption of vinyl groups was observed concurrently with formation of organic nitrates in RAIRS. XPS spectra showed little oxidation of sulfur head groups. The observed rate constant was determined based on the consumption of carbon-carbon double bonds and the formation of organic nitrates. Using this rate constant, the initial reaction probability was determined to be (3 ± 1) X 10-3. This reaction probability is approximately two orders of magnitude higher than that for the
reactions between the same surface and pure O3, which is due to the higher electron affinity of NO3 relative to O3. These results led to the development of a proposed mechanism that involves electrophilic addition of NO3 to the double bonds. Reactions between NO3 and a methyl-terminated SAM were also monitored in situ with RAIRS. In the CH3-SAM studies, hydrogen abstraction was observed during NO3 exposure. The results presented in this thesis should help develop an understanding of the
fundamental interfacial reaction dynamics of NO3 radicals with organic surfaces.
Advisors/Committee Members: Morris, John R. (committeechair), Troya, Diego (committee member), Tanko, James M. (committee member), Tissue, Brian M. (committee member).
Subjects/Keywords: nitrate radicals; self-assembled monolayers; ultrahigh vacuum; electrophilic addition; hydrogen abstraction
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APA (6th Edition):
Zhang, Y. (2012). Fundamental Studies of Reactions between NO3 Radicals and Organic Surfaces. (Masters Thesis). Virginia Tech. Retrieved from http://hdl.handle.net/10919/42372
Chicago Manual of Style (16th Edition):
Zhang, Yafen. “Fundamental Studies of Reactions between NO3 Radicals and Organic Surfaces.” 2012. Masters Thesis, Virginia Tech. Accessed January 17, 2021.
http://hdl.handle.net/10919/42372.
MLA Handbook (7th Edition):
Zhang, Yafen. “Fundamental Studies of Reactions between NO3 Radicals and Organic Surfaces.” 2012. Web. 17 Jan 2021.
Vancouver:
Zhang Y. Fundamental Studies of Reactions between NO3 Radicals and Organic Surfaces. [Internet] [Masters thesis]. Virginia Tech; 2012. [cited 2021 Jan 17].
Available from: http://hdl.handle.net/10919/42372.
Council of Science Editors:
Zhang Y. Fundamental Studies of Reactions between NO3 Radicals and Organic Surfaces. [Masters Thesis]. Virginia Tech; 2012. Available from: http://hdl.handle.net/10919/42372
University of Kansas
20.
Neal, Brad M.
Self-Assembly of Azulenic Monolayer Films on Metallic Gold Surfaces.
Degree: PhD, Chemistry, 2012, University of Kansas
URL: http://hdl.handle.net/1808/10458
► Derivatives of azulene, an unusual nonbenzenoid aromatic hydrocarbon featuring fused five- and seven-membered sp2 carbon rings, are of substantial current interest in the design of…
(more)
▼ Derivatives of azulene, an unusual nonbenzenoid aromatic hydrocarbon featuring fused five- and seven-membered sp2 carbon rings, are of substantial current interest in the design of advanced functional materials such as organic electronics. The overarching theme of this dissertation is the chemistry of new hybrid metal/azulene platforms featuring azulenic monolayer films anchored to the atomically flat metallic gold via isocyanide or thiolate junctions. One of the key aspects of this work addresses the influence of the junction group's nature on the formation, stability, reactivity, and physicochemical characteristics of azulenic
self-
assembled monolayer (SAM) films on the Au(111) surface. Both isocyanide and thiolate "alligator clips" in these SAMs were shown to induce approximately upright orientation of the azulenic framework with respect to the gold surface. The films' properties were evaluated by various means including surface-enhanced infrared spectroscopy and optical ellipsometry. Of particular significance are the initial examples of molecular films involving all three possible linear biazulenic scaffolds. These SAMs feature isocyanide junction groups and exhibit good stability toward ambient lighting and, in some cases, air atmosphere. Characterization of certain isocyano- and mercaptoazulenic molecular films reported herein was greatly facilitated by incorporation of ancillary nitrile infrared spectroscopic reporters into the azulenic scaffold. Such an approach constitutes a novel strategy in the surface chemistry of organic thiols, to the best of the author's knowledge. In addition, the thesis describes, among other isocyanide/thiol competitive binding investigations, the first monolayer film displacement studies involving the RSH and RNC species with identical substituents R that underscore the higher affinity of the thiolate versus isocyanide junctions for metallic gold. Accessibility of the new monolayer films reported in this dissertation paves the way for future experimental validation of the theoretically predicted low resistivity of the linear 2,6-azulenic framework.
Advisors/Committee Members: Barybin, Mikhail V. (advisor), Berrie, Cindy L. (advisor), Camarda, Kyle V. (cmtemember), Jackson, Timothy A. (cmtemember), Ren, Shenqianq (cmtemember).
Subjects/Keywords: Inorganic chemistry; Au(111); Azulene; Isocyanide; Self-assembled monolayers; Thiol
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APA (6th Edition):
Neal, B. M. (2012). Self-Assembly of Azulenic Monolayer Films on Metallic Gold Surfaces. (Doctoral Dissertation). University of Kansas. Retrieved from http://hdl.handle.net/1808/10458
Chicago Manual of Style (16th Edition):
Neal, Brad M. “Self-Assembly of Azulenic Monolayer Films on Metallic Gold Surfaces.” 2012. Doctoral Dissertation, University of Kansas. Accessed January 17, 2021.
http://hdl.handle.net/1808/10458.
MLA Handbook (7th Edition):
Neal, Brad M. “Self-Assembly of Azulenic Monolayer Films on Metallic Gold Surfaces.” 2012. Web. 17 Jan 2021.
Vancouver:
Neal BM. Self-Assembly of Azulenic Monolayer Films on Metallic Gold Surfaces. [Internet] [Doctoral dissertation]. University of Kansas; 2012. [cited 2021 Jan 17].
Available from: http://hdl.handle.net/1808/10458.
Council of Science Editors:
Neal BM. Self-Assembly of Azulenic Monolayer Films on Metallic Gold Surfaces. [Doctoral Dissertation]. University of Kansas; 2012. Available from: http://hdl.handle.net/1808/10458
University of New South Wales
21.
Lin, Yu-Shan.
Novel modified electrodes for sensing and protein electrochemistry.
Degree: Chemistry, 2012, University of New South Wales
URL: http://handle.unsw.edu.au/1959.4/52153
;
https://unsworks.unsw.edu.au/fapi/datastream/unsworks:10823/SOURCE01?view=true
► The research herein reports the characterization and development of a novel modified gold electrode consisting of gold nanoparticles attached to the end of a 11-amino-1-undecanethiol…
(more)
▼ The research herein reports the characterization and development of a novel modified gold electrode consisting of gold nanoparticles attached to the end of a 11-amino-1-undecanethiol
self-
assembled monolayers (SAMs). Polycrystalline gold electrodes modified with a passivating SAM shows that the immobilization of nanoparticles on the SAM ‘switch on’ the electrochemistry. The electrical properties of this electrode-monolayer-nanoparticle system were further investigated. The onset of electrochemistry was not influenced by the size of nanoparticles with a range between 15 and 60 nm. Furthermore, the effect of the charge of gold nanoparticles was evaluated by grafting short chain alkanethiols on nanoparticles as it was hypothesized that the onset of electrochemistry was perhaps due to the redox species being adsorbed onto nanoparticles. However, the electrochemical reactivity was reestablished even with electrostatic charge repulsion between positively charged modified nanoparticles and ruthenium hexamine. Finally, the influence of the density of gold nanoparticles was investigated, showing that the coverage of nanoparticles is quite similar even though the electrochemical signal increases with the longer incubation between 10 seconds and 120 seconds. The investigation of protein electrochemistry by using horseradish peroxidase (HRP) immobilized on the electrode-monolayer-nanoparticle construct was reported. As the HRP immobilized on the nanoparticles, the electron transfer kinetics was faster than that of HRP on the SAM in the absence of particles. This indicated that the underlying construct of gold nanoparticles attached on SAMs has opened a conducting channel that facilitates electron transfer when HRP was immobilized on the gold nanoparticles. Furthermore, the electrochemistry of another approach upon covalent attachment of HRP on the nanoparticles was also examined, showing that the electron transfer rate was faster than that of the method for electrostatic binding of HRP on the surface of particles. This suggested that the immobilization of HRP by covalent attachment on this modified electrode has higher efficiency in electron transfer between the enzyme and the electrode.
Advisors/Committee Members: Gooding, J. Justin, Chemistry, Faculty of Science, UNSW.
Subjects/Keywords: Novel modified electrodes; sensing; protein; electrochemistry; self-assembled monolayers (SAMs); nanoparticles
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APA (6th Edition):
Lin, Y. (2012). Novel modified electrodes for sensing and protein electrochemistry. (Masters Thesis). University of New South Wales. Retrieved from http://handle.unsw.edu.au/1959.4/52153 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:10823/SOURCE01?view=true
Chicago Manual of Style (16th Edition):
Lin, Yu-Shan. “Novel modified electrodes for sensing and protein electrochemistry.” 2012. Masters Thesis, University of New South Wales. Accessed January 17, 2021.
http://handle.unsw.edu.au/1959.4/52153 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:10823/SOURCE01?view=true.
MLA Handbook (7th Edition):
Lin, Yu-Shan. “Novel modified electrodes for sensing and protein electrochemistry.” 2012. Web. 17 Jan 2021.
Vancouver:
Lin Y. Novel modified electrodes for sensing and protein electrochemistry. [Internet] [Masters thesis]. University of New South Wales; 2012. [cited 2021 Jan 17].
Available from: http://handle.unsw.edu.au/1959.4/52153 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:10823/SOURCE01?view=true.
Council of Science Editors:
Lin Y. Novel modified electrodes for sensing and protein electrochemistry. [Masters Thesis]. University of New South Wales; 2012. Available from: http://handle.unsw.edu.au/1959.4/52153 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:10823/SOURCE01?view=true
University of St. Andrews
22.
Shen, Cai.
Self-assembled monolayers of thiolates as templates for micro/nano fabrication
.
Degree: 2008, University of St. Andrews
URL: http://hdl.handle.net/10023/603
► Self-assembled monolayers (SAMs) were investigated with regard to their application as templates to control processes down to the nanometre length scale. With applications of SAM…
(more)
▼ Self-
assembled monolayers (SAMs) were investigated with regard to their application as templates to control processes down to the nanometre length scale. With applications of SAM for electrochemical nanotechnology in mind, the range of aspects studied comprises patterning on different length scales, behaviour of SAMs under the conditions of electrochemical metal deposition, and the influence of the head and tail groups on formation and structure of SAMs.
On a micrometre scale, laser scanning lithography (LSL) was used to pattern SAM covered Au surfaces. With this technique, localized regions of a SAM are desorbed by scanning the focal spot of a laser beam. Thermal desorption occurs as a result of the high substrate temperature produced by the laser pulses. Patterns with line width as small as 0.9 µm were produced by LSL. It is demonstrated that SAM can not only be patterned by laser radiation but can also be rendered more passive as revealed by electrochemical metal deposition. Such blocking effect is explained by annealing of defects upon irradiation at the appropriate laser energy. This effect can block deposition of bulk copper particles, but does not prevent the underpotential deposition. Based on this passivation effect, large passivation areas can be created, which can be used as substrate for further nano/micro fabrication. The combination of SAM patterning and electrochemical metal deposition was also demonstrated to be an effective way to prepare superhydrophobic surfaces, exhibiting a contact angle of 165° (water droplet).
Aiming for the generation of smaller structures, scanning tunneling microscopy (STM) is used as a tool to pattern SAMs. Several phenomena observed in STM based manipulation of SAMs are addressed. The first one is sweeping effect. Deposited metal particles on top of SAM and SAMs are swept by STM tip by choosing appropriate I/V parameters. The closer the tip (higher current, lower bias), the more effective it is. Molecularly resolved images confirm that after sweeping, the scanned area is still covered by SAM molecules. This is explained by diffusion. The sweeping process can be repeated, thus, resulting in a layer by layer etching. The second effect is field-induced desorption. Applying a positive voltage (2.5-5V), a SAM is damaged beneath the area of the tip. The damage depends not only on the bias applied, but also on the current setpoint right before applying the bias. The third effect is nanografting. Nanografting was observed that a SAM having a stronger assembling ability can replace the weaker one (matrix layer) in hexadecane solution by STM scanning under normal I/V parameters combination that are usually used for imaging. It is found that longer chain can replace the shorter chain thiol, alkanethiol can replace biphenyl thiol. This method can be applied to pattern SAM.
Defects (punched holes) were created purposely on the SAMs covered Au surface and in situ STM was used to investigate the process of Under-Potential Deposition (UPD) and bulk metal deposition. Bulk metal…
Advisors/Committee Members: Buck, Manfred (advisor).
Subjects/Keywords: Self-assembled monolayers;
Electrochemical nanotechnology
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APA (6th Edition):
Shen, C. (2008). Self-assembled monolayers of thiolates as templates for micro/nano fabrication
. (Thesis). University of St. Andrews. Retrieved from http://hdl.handle.net/10023/603
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Shen, Cai. “Self-assembled monolayers of thiolates as templates for micro/nano fabrication
.” 2008. Thesis, University of St. Andrews. Accessed January 17, 2021.
http://hdl.handle.net/10023/603.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Shen, Cai. “Self-assembled monolayers of thiolates as templates for micro/nano fabrication
.” 2008. Web. 17 Jan 2021.
Vancouver:
Shen C. Self-assembled monolayers of thiolates as templates for micro/nano fabrication
. [Internet] [Thesis]. University of St. Andrews; 2008. [cited 2021 Jan 17].
Available from: http://hdl.handle.net/10023/603.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Shen C. Self-assembled monolayers of thiolates as templates for micro/nano fabrication
. [Thesis]. University of St. Andrews; 2008. Available from: http://hdl.handle.net/10023/603
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
NSYSU
23.
Han, Wen-hsin.
Effective Base-pair Mismatch Discrimination by Surface bound Nucleic Acid Probes and Atomic Force Microscope.
Degree: Master, Chemistry, 2009, NSYSU
URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0724109-120433
► Improving the identification ability of surfaced-immobilized nucleic acid probes for small size DNA or RNA targets, utilizing optical or electrochemical methods, has been the goal…
(more)
▼ Improving the identification ability of surfaced-immobilized nucleic acid probes for small size DNA or RNA targets, utilizing optical or electrochemical methods, has been the goal for the gene chip technology. This study focuses on new probe design for introducing hairpin structural features and locked nucleic acid modification. We use three kinds of probes (DNA-LN, DNA-HP and LNA-HP) to prepare recognition layers via
self-assembly processes on a gold substrate, and utilize AFM-based nanolithography technique to produce nanofeatures to observe the stiffness changes of oligonucleotide chains resulting from the formation of rigid double stranded duplexes when target sequence hybridizes to the probe. We also monitor the topographic changes upon exposure to the single mismatched and non-complementary targets as a function of time. The results reveal LNA-HP probes exhibit the highest response to discriminating single-point mutation in the base sequence. In addition, we study the effects of salt concentration, reaction temperature and the small size on the hybridization efficiency.
Advisors/Committee Members: Cheng-Lung Chen (chair), Chao-Ming Chiang (committee member), Shu-Chen Hsieh (chair).
Subjects/Keywords: mismatched hybridization; DNA self-assembled monolayers; DNA hibridization; locked nucleic acid; atomic force microscope
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APA (6th Edition):
Han, W. (2009). Effective Base-pair Mismatch Discrimination by Surface bound Nucleic Acid Probes and Atomic Force Microscope. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0724109-120433
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Han, Wen-hsin. “Effective Base-pair Mismatch Discrimination by Surface bound Nucleic Acid Probes and Atomic Force Microscope.” 2009. Thesis, NSYSU. Accessed January 17, 2021.
http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0724109-120433.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Han, Wen-hsin. “Effective Base-pair Mismatch Discrimination by Surface bound Nucleic Acid Probes and Atomic Force Microscope.” 2009. Web. 17 Jan 2021.
Vancouver:
Han W. Effective Base-pair Mismatch Discrimination by Surface bound Nucleic Acid Probes and Atomic Force Microscope. [Internet] [Thesis]. NSYSU; 2009. [cited 2021 Jan 17].
Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0724109-120433.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Han W. Effective Base-pair Mismatch Discrimination by Surface bound Nucleic Acid Probes and Atomic Force Microscope. [Thesis]. NSYSU; 2009. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0724109-120433
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
NSYSU
24.
Tang, Chih-Yi.
Development of a High-sensitivity Flexural Plate-wave Based Biosensor for Carcinoembryonic Antigen Detection.
Degree: Master, Electrical Engineering, 2016, NSYSU
URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0731116-092305
► According to the World Cancer Report 2014 by the International Agency for Research on Cancer (IARC), the global number of cases of colorectal cancer increased…
(more)
▼ According to the World Cancer Report 2014 by the International Agency for Research on Cancer (IARC), the global number of cases of colorectal cancer increased from 1.23 million in 2008 to 1.4 million in 2012. The colorectal cancer has also become the third major cancer killer in Taiwan. Carcinoembryonic Antigen (CEA) is a common tumor markers, which is secreted and released into the blood by the tumor cells. When the tumor cells form, the concentration of CEA will also increase in the blood. Currently, the main purpose of CEA in clinical is to keep tracking and detecting of colorectal cancer. However, the traditional CEA detectors usually have disadvantages of huge size, high cost, long detection time, etc. In order to detect colorectal cancer rapidly, a flexural plate-wave (FPW) biosensor for detecting CEA is developed in this thesis.
A FPW transducer was fabricated using micro-electro-mechanical systems (MEMS) technologies. The main fabrication steps of the proposed FPW devices include seven thin-film depositions and five photolithography processes. To accurately control the thickness of the silicon thin-plate and substantially improve the fabrication yield of FPW transducers, a two-step anisotropic wet etching process was also developed. Furthermore, by using cystamine-based
self-
assembled monolayer (SAM) nanotechnology, we used the proposed FPW device to develop a novel FPW-based carcinoembryonic antigen (CEA) biosensor for detection of colorectal cancer.
The implemented FPW device with dimension only about 0.56Ã0.56 cm2 and the thickness of suspended silicon plate can be accurately controlled to 20 μm. Under the optimized conditions, a very low insertion loss (-38.652 dB), very high mass sensitivity (126.67 cm2 /g) and high fabrication yield (60.78%) can be obtained. Furthermore, the proposed FPW based CEA-biosensor demonstrated a very low detection limit (5 ng/mL) and high mass-sensing linearity (0.96).
Advisors/Committee Members: Jin-Chern Chiou (chair), I-yu Huang (committee member), Yu-Cheng Lin (chair).
Subjects/Keywords: CEA-biosensors; Bulk Micromachining; Self-Assembled Monolayers; SAMs; flexural plate-wave; Insertion Loss
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APA (6th Edition):
Tang, C. (2016). Development of a High-sensitivity Flexural Plate-wave Based Biosensor for Carcinoembryonic Antigen Detection. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0731116-092305
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Tang, Chih-Yi. “Development of a High-sensitivity Flexural Plate-wave Based Biosensor for Carcinoembryonic Antigen Detection.” 2016. Thesis, NSYSU. Accessed January 17, 2021.
http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0731116-092305.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Tang, Chih-Yi. “Development of a High-sensitivity Flexural Plate-wave Based Biosensor for Carcinoembryonic Antigen Detection.” 2016. Web. 17 Jan 2021.
Vancouver:
Tang C. Development of a High-sensitivity Flexural Plate-wave Based Biosensor for Carcinoembryonic Antigen Detection. [Internet] [Thesis]. NSYSU; 2016. [cited 2021 Jan 17].
Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0731116-092305.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Tang C. Development of a High-sensitivity Flexural Plate-wave Based Biosensor for Carcinoembryonic Antigen Detection. [Thesis]. NSYSU; 2016. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0731116-092305
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
25.
Laaksonen, Timo.
Monolayer Protected Clusters: Electrostatics, Stability and Applications.
Degree: 2007, Helsinki University of Technology
URL: http://lib.tkk.fi/Diss/2007/isbn9789512288861/
► Monolayer protected clusters (MPCs) have become one of the most actively researched areas in chemistry. They have the potential to be the next step in…
(more)
▼ Monolayer protected clusters (MPCs) have become one of the most actively researched areas in chemistry. They have the potential to be the next step in creating building blocks for future highly miniaturised electric devices and could be used in biosciences as effective drug carriers and sensors. This thesis showcases some physico-chemical aspects and applications of these particles and attempts to provide tools and ideas for further research in this expanding field. In this thesis, electrochemical studies showed that MPCs can have as many as 15 distinctive charge states and that even a HOMO-LUMO gap can be measured at room temperature for sufficiently small nanoparticles. Self-assembled monolayers on the nanoparticle surface were shown to be ion permeable, and this can radically affect the charging phenomenon. Degradation of the single electron transfer was observed with small counter-ions, which can penetrate into the monolayer and increase its capacitance. In another study, ion rectified MPC film charging response was shown to be due to ion solvation phenomena and could be treated analogously to a liquid|liquid interface, with charging onset potential dependent on the standard ion transfer potential of the counter-ion across the film|solution interface. Aqueous stability of charged MPCs was also considered in the thesis. The solubility was demonstrated to be practically independent of the ionic strength of the solution for salts comprised of large counter-ions. These ions were shown to form an electrostatically bound steric barrier around the nanoparticle. Such solubility issues are very relevant for biosensor applications where typically aqueous dispersion of nanoparticles are used. Dithiol place exchange of the MPC bound thiol is an interesting reaction and often used in the formation of MPC superstructures. Here, aggregation induced by interlinking MPCs with hexanedithiol was studied by dynamic light scattering and fitted to a simple kinetic model. This provided an indirect means to link the ligand exchange to the aggregation kinetics. Finally, an application of gold nanoparticles to drug delivery is presented. Liposomes loaded with Au-MPCs and marker molecules were shown to be relatively stable and released their contents with an external signal i.e. laser irradiation. The technique would be an excellent option for drug delivery in particular in ocular medicine.
Yksikerroksella suojatuista klustereista (Monolayer Protected Clusters, MPC) on tullut yksi eniten tutkittu kemian ja fysiikan alue. Niille on suunniteltu useita merkittäviä sovelluksia tulevaisuudessa; Ne voisivat muun muassa olla seuraava askel elektronisten komponenttien pienentämisessä. Jo nyt niitä voidaan käyttää biotieteissä lääkkeiden kuljetuksessa ja bioantureissa. Tässä väitöskirjassa sähkökemialliset tutkimukset osoittivat, että MPC:llä voi olla jopa 15 eri varaustilaa ja että HOMO-LUMO väli voidaan mitata huoneenlämmössä tarpeeksi pienillä nanopartikkeleilla. Työssä osoitettiin, että varsinkin pienet ionit pystyvät läpäisemään…
Advisors/Committee Members: Helsinki University of Technology, Department of Chemical Technology, Laboratory of Physical Chemistry and Electrochemistry.
Subjects/Keywords: nanoparticles; electrochemistry; self-assembled monolayers; liposomes; ligand exchange; nanopartikkelit; sähkökemia; itsejärjestäytyneet yksikerrokset; liposomit; ligandinvaihto
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APA ·
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APA (6th Edition):
Laaksonen, T. (2007). Monolayer Protected Clusters: Electrostatics, Stability and Applications. (Thesis). Helsinki University of Technology. Retrieved from http://lib.tkk.fi/Diss/2007/isbn9789512288861/
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Laaksonen, Timo. “Monolayer Protected Clusters: Electrostatics, Stability and Applications.” 2007. Thesis, Helsinki University of Technology. Accessed January 17, 2021.
http://lib.tkk.fi/Diss/2007/isbn9789512288861/.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Laaksonen, Timo. “Monolayer Protected Clusters: Electrostatics, Stability and Applications.” 2007. Web. 17 Jan 2021.
Vancouver:
Laaksonen T. Monolayer Protected Clusters: Electrostatics, Stability and Applications. [Internet] [Thesis]. Helsinki University of Technology; 2007. [cited 2021 Jan 17].
Available from: http://lib.tkk.fi/Diss/2007/isbn9789512288861/.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Laaksonen T. Monolayer Protected Clusters: Electrostatics, Stability and Applications. [Thesis]. Helsinki University of Technology; 2007. Available from: http://lib.tkk.fi/Diss/2007/isbn9789512288861/
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Rochester
26.
Lyubarskaya, Yekaterina Leonidovna (1986 -
); Shestopalov, Alexander A.
Tuning surface properties using self-assembled monolayers
for various applications.
Degree: PhD, 2014, University of Rochester
URL: http://hdl.handle.net/1802/28843
► The research presented in this dissertation focuses on the study of self-assembled monolayers (SAMs) in the modification of surface properties of different substrates for various…
(more)
▼ The research presented in this dissertation focuses
on the study of self-assembled monolayers (SAMs) in the
modification of surface properties of different substrates for
various applications. Self-assembled monolayers are organic
molecules that can be deposited on a variety of surfaces, such as
those of metals, metal-oxides, and semiconductors. Formation of
SAMs on any inorganic material provides a ubiquitous way to impart
desirable chemical and physical properties of organic and
biological molecules to the inorganic substrate.
</br>
It has been demonstrated that single
molecules and their self-assembled monolayers can significantly
alter the physical and electronic properties of inorganic
conductors; moreover, studies have shown that the performance of
many electrical devices can be transformed by modifying inorganic
electrodes with organic SAMs. This is especially important for the
development of next generation of ultra-compact electronic devices,
in which the ability to control the interfacial charge-transport
with a single monolayer of organic molecules would be ideal. We
have developed different organic electronic architectures as test
beds for studying the effect of monolayer properties, such as
structural and geometrical parameters, on their electronic
properties. By using a typical organic electronic device as a
sensitive test platform, slight changes in a monolayer property,
such as length, have been detected by studying the current-
voltage
characteristics (JV) of organic diodes functionalized with
self-assembled monolayers (SAMs) of varying alkyl chain-length.
Next, we describe the application of SAMs based on
n-octylphosphonic acid (C8PA) and
1H,1H,2H,2H-perfluorooctanephosphonic acid (PFOPA) as anode buffer
layers in C60-based organic photovoltaic
(OPV) devices. We used the OPV platform to compare stabilities of
organic monolayers exposed to ambient conditions with SAMs
positioned inside working OPV devices. We found that the
stabilities are different, suggesting the degradation mechanisms
are distinct. The degradation of the OPV efficiency with respect to
air exposure was significantly reduced with the perfluorinated
PFOPA compared to the aliphatic C8PA. We attributed the OPV
degradation to moisture diffusion from the top aluminum electrode
and we discuss that the lowering of the anode work function is the
result of hydrolysis of the SAM buffer layer. </br>
Next, we demonstrated the dependence of molecular electronic
properties on the functional group substitution and that the
changes in these properties can be measured using the organic
light-emitting (OLED) platform. Specifically, we compared bilayered
organic monomolecular systems immobilized on an inorganic electrode
as the charge-injecting components of the organic light emitting
diodes (OLEDs). Our bilayered interfaces comprise ordered inert
primary and functional reactive layers, and they differ in only one
parameter: the molecular structure of the terminal functional
group. We demonstrate that we can visualize the…
Subjects/Keywords: Janus particles; Molecular electronics; Organic light-emitting diodes; Organic photovoltaic devices; Self-assembled monolayers
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APA (6th Edition):
Lyubarskaya, Y. L. (. -. (2014). Tuning surface properties using self-assembled monolayers
for various applications. (Doctoral Dissertation). University of Rochester. Retrieved from http://hdl.handle.net/1802/28843
Chicago Manual of Style (16th Edition):
Lyubarskaya, Yekaterina Leonidovna (1986 -. “Tuning surface properties using self-assembled monolayers
for various applications.” 2014. Doctoral Dissertation, University of Rochester. Accessed January 17, 2021.
http://hdl.handle.net/1802/28843.
MLA Handbook (7th Edition):
Lyubarskaya, Yekaterina Leonidovna (1986 -. “Tuning surface properties using self-assembled monolayers
for various applications.” 2014. Web. 17 Jan 2021.
Vancouver:
Lyubarskaya YL(-. Tuning surface properties using self-assembled monolayers
for various applications. [Internet] [Doctoral dissertation]. University of Rochester; 2014. [cited 2021 Jan 17].
Available from: http://hdl.handle.net/1802/28843.
Council of Science Editors:
Lyubarskaya YL(-. Tuning surface properties using self-assembled monolayers
for various applications. [Doctoral Dissertation]. University of Rochester; 2014. Available from: http://hdl.handle.net/1802/28843
27.
Coelho, Dyovani.
Caracterização eletroquímica de uma monocamada auto-organizada mista composta por ácido 3-mercaptopropiônico e ácido 11-mercaptoundecanóico.
Degree: Mestrado, Química Analítica, 2011, University of São Paulo
URL: http://www.teses.usp.br/teses/disponiveis/75/75132/tde-20062011-150712/
;
► Monocamadas auto-organizadas formadas pela quimissorção de alcanotióis sobre ouro apresentam estruturas bem definidas, organizadas e reprodutíveis. As propriedades de SAMs, aliadas à facilidade de síntese,…
(more)
▼ Monocamadas auto-organizadas formadas pela quimissorção de alcanotióis sobre ouro apresentam estruturas bem definidas, organizadas e reprodutíveis. As propriedades de SAMs, aliadas à facilidade de síntese, têm atraído o interesse da comunidade cientifica o que ocasionou um grande avanço na área de nanotecnologia, especialmente em engenharia de superfícies. Neste trabalho, avaliou-se a modificação de um substrato de ouro com uma mistura dos ácidos 3-mercaptopropiônico e 11-mercaptoundecanóico. Com isso procurou-se controlar a configuração da superfície da SAM a fim de se obter um arranjo com ilhas de 3amp circundadas por 11amu, similar a um conjunto de ultramicroeletrodos. A SAM mista foi produzida por incubação de um substrato de ouro em solução etanólica contendo ambos os tiois. O estudo do processo redox do par [Fe(CN)6]4-/[Fe(CN)6]3- demonstrou que monocamadas de 3amp se comportam como a superfície de ouro não modificado, apresentando os mesmos valores de corrente de pico (Ip), potencial de pico (Ep) e resistência de transferência de carga (Rtc), pois permitem que ocorra a transferência eletrônica por efeito de tunelamento quântico dos elétrons através da monocamada. Entretanto monocamadas de 11amu demonstram comportamento isolante, apresentando uma Rtc 250 vezes maior e Ip significativamente menores que a observada para a SAM-3amp. As cargas de dessorção redutiva e variação de massa obtidas com a MECQ para a SAM mista evidenciaram alto recobrimento da superfície e um mecanismo de adsorção que varia com o tempo de incubação do substrato em solução contendo os tiois. Assim, a configuração da superfície com domínios de 3amp bem estabelecidos é alcançada com 20 horas de incubação do substrato. O perfil observado com a voltametria cíclica, aliado aos dados obtidos com a EIE comprova a existência de segregação de fases na SAM mista contendo regiões recobertas pelo 3amp (raio médio de 4,3 µm) circundadas por 11amu (com separação média de 75,42 µm). O estudo do comportamento eletroquímico do fisetin confirma o surgimento de propriedades diferenciadas com a SAM mista, onde observou-se apenas um processo redox sem que o analito permanecesse adsorvido na superfície da SAM. Todavia para a SAM-3amp e ouro não modificado foi observado dois processos redoxes seguido de adsorção irreversível do produto da reação na superfície eletródica. Utilizando a SAM mista como sensor eletroquímico para determinação de fisetin em água alcançou-se um limite de detecção de 1,67 x10-8 mol L-1.
Self-assembled monolayers obtained by chemiosorption of alkanethiols on gold present well-defined, organized and reproducible structures. The unique properties exhibited by such surfaces, allied to the great facility to obtain, have attracted the interest of the scientific community and caused a significant advance in the nanotechnology research, especially in the surface engineering one. In this work, the modification of gold substrate with a mixture of 3-mercaptopropionic acid and 11-mercaptoundecanoic…
Advisors/Committee Members: Machado, Sergio Antonio Spinola.
Subjects/Keywords: Alcanotiois; Alkanethiols; Mixed-SAM; Monocamadas auto-organizadas; SAM mista; Self-assembled monolayers
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APA ·
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MLA ·
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CSE |
Export
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Manager
APA (6th Edition):
Coelho, D. (2011). Caracterização eletroquímica de uma monocamada auto-organizada mista composta por ácido 3-mercaptopropiônico e ácido 11-mercaptoundecanóico. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/75/75132/tde-20062011-150712/ ;
Chicago Manual of Style (16th Edition):
Coelho, Dyovani. “Caracterização eletroquímica de uma monocamada auto-organizada mista composta por ácido 3-mercaptopropiônico e ácido 11-mercaptoundecanóico.” 2011. Masters Thesis, University of São Paulo. Accessed January 17, 2021.
http://www.teses.usp.br/teses/disponiveis/75/75132/tde-20062011-150712/ ;.
MLA Handbook (7th Edition):
Coelho, Dyovani. “Caracterização eletroquímica de uma monocamada auto-organizada mista composta por ácido 3-mercaptopropiônico e ácido 11-mercaptoundecanóico.” 2011. Web. 17 Jan 2021.
Vancouver:
Coelho D. Caracterização eletroquímica de uma monocamada auto-organizada mista composta por ácido 3-mercaptopropiônico e ácido 11-mercaptoundecanóico. [Internet] [Masters thesis]. University of São Paulo; 2011. [cited 2021 Jan 17].
Available from: http://www.teses.usp.br/teses/disponiveis/75/75132/tde-20062011-150712/ ;.
Council of Science Editors:
Coelho D. Caracterização eletroquímica de uma monocamada auto-organizada mista composta por ácido 3-mercaptopropiônico e ácido 11-mercaptoundecanóico. [Masters Thesis]. University of São Paulo; 2011. Available from: http://www.teses.usp.br/teses/disponiveis/75/75132/tde-20062011-150712/ ;
28.
Galli, Andressa.
Desenvolvimento e caracterização de um biossensor bienzimático imobilizado sobre monocamadas auto-organizadas para determinação de açúcares em alimentos.
Degree: PhD, Química Analítica, 2009, University of São Paulo
URL: http://www.teses.usp.br/teses/disponiveis/75/75132/tde-13112009-084320/
;
► Este trabalho descreve a preparação, a caracterização e o uso de um biossensor bienzimático confeccionado com as enzimas glicose oxidase e frutose dehidrogenase imobilizadas em…
(more)
▼ Este trabalho descreve a preparação, a caracterização e o uso de um biossensor bienzimático confeccionado com as enzimas glicose oxidase e frutose dehidrogenase imobilizadas em camadas auto-organizadas ou self-assembled monolayers (SAMs) de cistamina para a quantificação de açúcares em alimentos. Após o preparo do eletrodo de ouro com a SAM de cistamina, biossensores foram construídos e para obtenção de melhores respostas, condições foram otimizadas, tais como: concentração do mediador tetratiafulvaleno (TTF), porcentagem de glutaraldeído, temperatura e tempo de vida do biossensor. Com as condições estabelecidas, fez-se então, a determinação analítica da D-glicose e da D-frutose em eletrólito puro pelo método da adição de padrão e os resultados foram obtidos por voltametria cíclica e cronoamperometria. A corrente de pico de oxidação do mediador de elétrons (TTF) aumentou proporcionalmente com o aumento da concentração e não ocorreram deslocamentos nos potenciais de pico. Os limites de detecção (LD) foram encontrados por meio do desvio padrão da média aritmética de dez amperogramas do branco no potencial equivalente aos dos picos de oxidação do mediador de elétrons TTF, juntamente com o valor do coeficiente angular da curva analítica. Após a obtenção da curva analítica o biossensor foi aplicado diretamente em amostras de refrigerante dietético e não dietético, bem como em amostras de adoçantes comerciais, onde foram realizados testes comparativos da resposta dos biossensores. Para o eletrólito puro e amostras de refrigerante dietético e de adoçantes, ou seja, onde não há presença de D-glicose e D-frutose, notou-se a ausência de corrente de pico, enquanto que para as amostras de refrigerante não dietético, houve um valor significativo de resposta de corrente, indicando a presença dos açúcares em estudo. Com o propósito de verificar a influência de interferentes e o efeito de matriz, foi construída uma curva analítica para a D-glicose e para a D-frutose, em amostras de refrigerante dietético e adoçantes, onde foram obtidas as menores quantidade destes açúcares. Para o refrigerante não dietético, foi determinado o valor inicial dos açúcares presentes nas amostras. Pode-se afirmar que a utilização dos biossensores baseados nas enzimas GOx e FDH mostraram-se eficientes para a determinação dos açúcares D-glicose e D-frutose nas amostras analisadas (com diferença significativa nos valores de corrente), apresentando uma resposta rápida, além da eliminação do efeito da matriz. A utilização do mediador de elétrons (TTF) possibilitou a reação em potencial próximo de zero, diminuindo o efeito de interferentes e evitando a desnaturação das enzimas.
This work describes the preparation, characterization and application of a bienzymatic biosensor based in the glucose oxidaze and fructose dehydrogenase immobilized on self-assembled monolayer of cystamine for sugar quantification in foodstuff. After the modification of the gold electrode with cystamine, the biosensors were developed and optimized for best responses. Optimization…
Advisors/Committee Members: Machado, Sergio Antonio Spinola.
Subjects/Keywords: camadas auto-organizadas; fructose dehidrogenase; frutose dehidrogenase; glicose oxidase; glucose oxidase; self assembled monolayers
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APA (6th Edition):
Galli, A. (2009). Desenvolvimento e caracterização de um biossensor bienzimático imobilizado sobre monocamadas auto-organizadas para determinação de açúcares em alimentos. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/75/75132/tde-13112009-084320/ ;
Chicago Manual of Style (16th Edition):
Galli, Andressa. “Desenvolvimento e caracterização de um biossensor bienzimático imobilizado sobre monocamadas auto-organizadas para determinação de açúcares em alimentos.” 2009. Doctoral Dissertation, University of São Paulo. Accessed January 17, 2021.
http://www.teses.usp.br/teses/disponiveis/75/75132/tde-13112009-084320/ ;.
MLA Handbook (7th Edition):
Galli, Andressa. “Desenvolvimento e caracterização de um biossensor bienzimático imobilizado sobre monocamadas auto-organizadas para determinação de açúcares em alimentos.” 2009. Web. 17 Jan 2021.
Vancouver:
Galli A. Desenvolvimento e caracterização de um biossensor bienzimático imobilizado sobre monocamadas auto-organizadas para determinação de açúcares em alimentos. [Internet] [Doctoral dissertation]. University of São Paulo; 2009. [cited 2021 Jan 17].
Available from: http://www.teses.usp.br/teses/disponiveis/75/75132/tde-13112009-084320/ ;.
Council of Science Editors:
Galli A. Desenvolvimento e caracterização de um biossensor bienzimático imobilizado sobre monocamadas auto-organizadas para determinação de açúcares em alimentos. [Doctoral Dissertation]. University of São Paulo; 2009. Available from: http://www.teses.usp.br/teses/disponiveis/75/75132/tde-13112009-084320/ ;
29.
Cancino, Juliana Carlos.
Eletrodos modificados com monocamadas auto-organizadas de alcanotióis: uma abordagem sobre a transferência eletrônica.
Degree: Mestrado, Química Analítica, 2008, University of São Paulo
URL: http://www.teses.usp.br/teses/disponiveis/75/75132/tde-03092008-172520/
;
► A modificação de eletrodos por sistemas auto-organizados (SAM) é um dos temas mais discutidos em eletroquímica e eletroanalítica, principalmente por elevar a biocompatibilidade destas superfícies.…
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▼ A modificação de eletrodos por sistemas auto-organizados (SAM) é um dos temas mais discutidos em eletroquímica e eletroanalítica, principalmente por elevar a biocompatibilidade destas superfícies. O foco principal deste trabalho está na transferência eletrônica através das SAMs de moléculas de alcanotióis sobre a superfície de eletrodos de ouro. Com a finalidade de investigar o comportamento eletroquímico do par redox [Fe(CN)6]3-/[Fe(CN)6]4-,, as superfícies de ouro foram modificadas com SAMs de alcanotióis com cadeias carbônicas de diferentes comprimentos e suas misturas. Desta forma, foi possível observar que as superfícies modificadas com monocamadas com 2, 3 ou 4 átomos de carbono em sua cadeia permitem que a transferência de elétrons ocorra através da SAM, produzindo perfis voltamétricos muito similares aos observados com a superfície de ouro sem a modificação. Com cadeias carbônicas longas (até 11 átomos de carbono), a transferência eletrônica é totalmente bloqueada e nenhum perfil voltamétrico foi observado. Com o objetivo de explorar o bloqueio total da superfície modificada de moléculas de alcanotióis de cadeias longas, os excessos superficiais foram calculados por meio da carga voltamétrica obtida da dessorção redutiva. Observaram-se valores de excesso superficial próximos a 9,0x10-10 mol/cm2, o que demonstra uma cobertura completa dos sítios ativos da superfície, justificando assim a ausência da resposta eletroquímica. A superfície modificada com as misturas de monocamadas de cadeias carbônicas longas e curtas promoveu respostas voltamétricas interessantes, desde que as moléculas de cadeias curtas contenham menos que 4 átomos de carbono. Neste caso, respostas voltamétricas sigmoidais similares às de ultramicroeletrodos (UMEs) foram obtidas. Este efeito foi associado com a formação de conjuntos de UMEs formados na superfície do eletrodo. O raio e a separação entre os UMEs nestas superfícies foram calculados como sendo 42 e 221 µm, respectivamente. Um total de 60 UMEs foi encontrado na superfície modificada. Estes UMEs foram formados por moléculas de cadeias menores totalmente emparedadas por cadeias maiores (moléculas de ácido 11- mercaptoundecanóico). Este comportamento reforça o propósito de que a transferência dos elétrons ocorra através das monocamadas de cadeia menor e que as moléculas de cadeia longa bloqueiam a superfície. Todos os experimentos voltamétricos foram seguidos de análises de espectroscopia de impedância eletroquímica os quais permitiram determinar a resistência de transferência de carga para cada modificação. Superfícies modificadas com alcanotióis de cadeia longa apresentaram elevados valores de transferência de carga, enquanto que as superfícies modificadas com alcanotióis de cadeia curta apresentam baixos valores de transferência eletrônica, em concordância com o comportamento voltamétrico observado. Finalmente, a organização de cada monocamada foi monitorada utilizando a microbalança eletroquímica de cristal de quartzo. Pela variação de massa com o tempo de imersão do eletrodo…
Advisors/Committee Members: Machado, Sergio Antonio Spinola.
Subjects/Keywords: alcanotióis; alkanothiols; eletrodos modificados; modified electrodes; monocamadas auto-organizadas; self-assembled monolayers
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
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Manager
APA (6th Edition):
Cancino, J. C. (2008). Eletrodos modificados com monocamadas auto-organizadas de alcanotióis: uma abordagem sobre a transferência eletrônica. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/75/75132/tde-03092008-172520/ ;
Chicago Manual of Style (16th Edition):
Cancino, Juliana Carlos. “Eletrodos modificados com monocamadas auto-organizadas de alcanotióis: uma abordagem sobre a transferência eletrônica.” 2008. Masters Thesis, University of São Paulo. Accessed January 17, 2021.
http://www.teses.usp.br/teses/disponiveis/75/75132/tde-03092008-172520/ ;.
MLA Handbook (7th Edition):
Cancino, Juliana Carlos. “Eletrodos modificados com monocamadas auto-organizadas de alcanotióis: uma abordagem sobre a transferência eletrônica.” 2008. Web. 17 Jan 2021.
Vancouver:
Cancino JC. Eletrodos modificados com monocamadas auto-organizadas de alcanotióis: uma abordagem sobre a transferência eletrônica. [Internet] [Masters thesis]. University of São Paulo; 2008. [cited 2021 Jan 17].
Available from: http://www.teses.usp.br/teses/disponiveis/75/75132/tde-03092008-172520/ ;.
Council of Science Editors:
Cancino JC. Eletrodos modificados com monocamadas auto-organizadas de alcanotióis: uma abordagem sobre a transferência eletrônica. [Masters Thesis]. University of São Paulo; 2008. Available from: http://www.teses.usp.br/teses/disponiveis/75/75132/tde-03092008-172520/ ;
Temple University
30.
Redhu, Shiv Kumar.
PROPERTIES AND MOLECULAR INTERACTIONS OF TWO-DIMENSIONAL NUCLEIC ACID NANOASSEMBLIES: IMPLICATIONS FOR BIOSENSING AND DIAGNOSTICS.
Degree: PhD, 2014, Temple University
URL: http://digital.library.temple.edu/u?/p245801coll10,246293
► Chemistry
There is a need for the development of new technologies for the early detection of disease. Diverse initiatives are underway in academia and the…
(more)
▼ Chemistry
There is a need for the development of new technologies for the early detection of disease. Diverse initiatives are underway in academia and the pharmaceutical and biotechnology industries to develop highly-sensitive, high-throughput methods to detect disease-relevant biomarkers at the single-cell level. Biomarkers can define the progress of a disease or efficacy of disease treatment, and can include nucleic acids (RNA, DNA), proteins, small molecules, or even specific cells. While discovery research in this area is accelerating, there are a number of current experimental limitations. Most existing methodologies require a relatively large sample size. Also, amplification-based detection technologies are destructive to sample, and errors in amplification can occur, leading to an incorrect diagnosis. Nanomaterial-based devices (nanodevices) offer the promise of label-free, amplification-free detection strategies. Such nanodevices could allow analysis of minute biological samples without the requirement for amplification or incorporation of reporter groups. Loss of sample, due to handling and processing would be minimized and the sample could be recovered for further analysis. Atomic force microscopy (AFM) allows topographic imaging and compressibility/elasticity measurement of biomolecules on solid supports. AFM can enable assays of ligand binding with single molecule detection capability. Certain nucleic acid types, in particular double-stranded (ds) RNA, can act as a biomarker for specific cancers (e.g. leukemia) and viral infection. dsRNA also is of interest since it is a conserved structural feature of precursors to gene-regulatory RNAs, including micro (mi) RNAs and short interfering (si) RNAs. This project demonstrates a single-step, label-free, amplification-free approach for detecting the interaction of biomolecules that bind and/or process dsRNA, using a nanomanipulated, self-assembled monolayer (SAM) of a ds[RNA-DNA] chimera as imprinting matrix, a reference nuclease as imprinting agent, and AFM for imprint-readout. The action of the dsRNA-specific enzyme, ribonuclease III (RNase III), as well as the binding of an inactive, dsRNA-binding RNase III mutant can be permanently recorded by the input-responsive action of a restriction endonuclease that cleaves an ancillary reporter site within the dsDNA segment. The resulting irreversible height change of the arrayed ds[RNA-DNA] chimera, as measured by atomic force microscopy, provides a distinct digital output for each type of input. These findings provide the basis for developing imprinting-based nano-biosensors, and reveal the versatility of AFM as a tool for characterizing the behaviour of highly-crowded biomolecules at the nanoscale. RNA-DNA heteroduplexes are biomarkers for specific inflammatory conditions of genetic origin, and also are the product of capture of an RNA (e.g., miRNA) by a complementary DNA sequence. The approach used here to detect RNA-DNA hybrids is based on the ability of alkylthiol-modified ssDNA molecules to form monolayers…
Advisors/Committee Members: Nicholson, Allen W.;, Castronovo, Matteo, Zdilla, Michael J., Andrade, Rodrigo B.;.
Subjects/Keywords: Chemistry; Biochemistry;
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APA ·
Chicago ·
MLA ·
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CSE |
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APA (6th Edition):
Redhu, S. K. (2014). PROPERTIES AND MOLECULAR INTERACTIONS OF TWO-DIMENSIONAL NUCLEIC ACID NANOASSEMBLIES: IMPLICATIONS FOR BIOSENSING AND DIAGNOSTICS. (Doctoral Dissertation). Temple University. Retrieved from http://digital.library.temple.edu/u?/p245801coll10,246293
Chicago Manual of Style (16th Edition):
Redhu, Shiv Kumar. “PROPERTIES AND MOLECULAR INTERACTIONS OF TWO-DIMENSIONAL NUCLEIC ACID NANOASSEMBLIES: IMPLICATIONS FOR BIOSENSING AND DIAGNOSTICS.” 2014. Doctoral Dissertation, Temple University. Accessed January 17, 2021.
http://digital.library.temple.edu/u?/p245801coll10,246293.
MLA Handbook (7th Edition):
Redhu, Shiv Kumar. “PROPERTIES AND MOLECULAR INTERACTIONS OF TWO-DIMENSIONAL NUCLEIC ACID NANOASSEMBLIES: IMPLICATIONS FOR BIOSENSING AND DIAGNOSTICS.” 2014. Web. 17 Jan 2021.
Vancouver:
Redhu SK. PROPERTIES AND MOLECULAR INTERACTIONS OF TWO-DIMENSIONAL NUCLEIC ACID NANOASSEMBLIES: IMPLICATIONS FOR BIOSENSING AND DIAGNOSTICS. [Internet] [Doctoral dissertation]. Temple University; 2014. [cited 2021 Jan 17].
Available from: http://digital.library.temple.edu/u?/p245801coll10,246293.
Council of Science Editors:
Redhu SK. PROPERTIES AND MOLECULAR INTERACTIONS OF TWO-DIMENSIONAL NUCLEIC ACID NANOASSEMBLIES: IMPLICATIONS FOR BIOSENSING AND DIAGNOSTICS. [Doctoral Dissertation]. Temple University; 2014. Available from: http://digital.library.temple.edu/u?/p245801coll10,246293
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