Advanced search options

Advanced Search Options 🞨

Browse by author name (“Author name starts with…”).

Find ETDs with:

in
/  
in
/  
in
/  
in

Written in Published in Earliest date Latest date

Sorted by

Results per page:

Sorted by: relevance · author · university · dateNew search

You searched for subject:(Sclerosants). Showing records 1 – 2 of 2 total matches.

Search Limiters

Last 2 Years | English Only

No search limiters apply to these results.

▼ Search Limiters


University of New South Wales

1. Cooley Andrade, Osvaldo. A Study of the Apoptotic Effects of Detergent Sclerosants on Vascular and Circulating Blood Cells.

Degree: Clinical School - St Vincent's Hospital, 2017, University of New South Wales

The in vitro effects of detergent sclerosants, sodium tetradecylsulphate (STS) andpolidocanol (POL) on cell activation, cytokine release, apoptosis and oncosis wereinvestigated. The general hypothesis of this thesis was that detergent sclerosants at lowconcentrations induce the release of proinflammatory and proangiogenic cytokines.Leukocytes, platelets, and endothelial cells (ECs) were incubated with varying concentrations(0%, 0.075%, 0.15%, 0.30%, 0.60% and 1.2%) of sclerosants. ELISA was used to detect therelease of proinflammatory and proangiogenic cytokines. Interferon-g and tumour necrosisfactor-a were released from STS-stimulated polymorphonuclear cells (PMNC). Bothsclerosants induced the release of vascular endothelial growth factor (VEGF).Morphological changes were analysed using fluorescence microscopy. Nuclear andcytoplasmic changes consistent with cell activation, apoptosis, oncosis and senescencewere found with both STS and POL at low concentrations (< 0.3%).Leukocyte and EC apoptosis was assessed using confocal microscopy and flow cytometry.Lactadherin and PI were used to stage apoptosis and Caspase-3, -8, -9 and Bax to assessapoptotic pathways. Porimin was used to assess oncosis. STS induced activation ofcaspase-3, -8, -9 and increase in Bax expression. POL only induced activation of Caspase-3, -9 and Bax. Active Caspase 3, 8 and 9 but not Bax were increased in EC stimulated withlow concentrations of both STS and POL. Both agents increased the activation of poriminat all concentrations.In Conclusion, detergent sclerosants induce cell activation and the release of proinflammatoryand proangiogenic cytokines. Sclerosants at low concentrations are also capable of inducingcell apoptosis and cell oncosis in leukocytes and EC. Advisors/Committee Members: Parsi, Kurosh, Faculty of Medicine, UNSW, Connor, David, Faculty of Medicine, UNSW.

Subjects/Keywords: Oncosis; Sclerosants; Apoptosis

Record DetailsSimilar RecordsGoogle PlusoneFacebookTwitterCiteULikeMendeleyreddit

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Cooley Andrade, O. (2017). A Study of the Apoptotic Effects of Detergent Sclerosants on Vascular and Circulating Blood Cells. (Doctoral Dissertation). University of New South Wales. Retrieved from http://handle.unsw.edu.au/1959.4/58361 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:45847/SOURCE02?view=true

Chicago Manual of Style (16th Edition):

Cooley Andrade, Osvaldo. “A Study of the Apoptotic Effects of Detergent Sclerosants on Vascular and Circulating Blood Cells.” 2017. Doctoral Dissertation, University of New South Wales. Accessed September 24, 2020. http://handle.unsw.edu.au/1959.4/58361 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:45847/SOURCE02?view=true.

MLA Handbook (7th Edition):

Cooley Andrade, Osvaldo. “A Study of the Apoptotic Effects of Detergent Sclerosants on Vascular and Circulating Blood Cells.” 2017. Web. 24 Sep 2020.

Vancouver:

Cooley Andrade O. A Study of the Apoptotic Effects of Detergent Sclerosants on Vascular and Circulating Blood Cells. [Internet] [Doctoral dissertation]. University of New South Wales; 2017. [cited 2020 Sep 24]. Available from: http://handle.unsw.edu.au/1959.4/58361 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:45847/SOURCE02?view=true.

Council of Science Editors:

Cooley Andrade O. A Study of the Apoptotic Effects of Detergent Sclerosants on Vascular and Circulating Blood Cells. [Doctoral Dissertation]. University of New South Wales; 2017. Available from: http://handle.unsw.edu.au/1959.4/58361 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:45847/SOURCE02?view=true


University of New South Wales

2. Parsi, Kurosh. Interaction of detergent sclerosants with coagulation, antithrombotic and fibrinolytic mecanisms.

Degree: Clinical School - St Vincent's Hospital, 2011, University of New South Wales

The effects of detergent sclerosants, sodium tetradecyl sulphate (STS) and polidocanol (POL), on coagulation, antithrombotic and fibrinolytic mechanisms were investigated in vitro.All samples were spiked with each sclerosant at therapeutic concentrations. Coagulation was investigated in clotting tests and functional assays for clotting factors in plasma. Fibrinogen was measured by the Clauss method and factor (F) XIII by ELISA. At low concentrations, sclerosants shortened phospholipid-dependent clotting times. At high concentrations, STS prolonged all clotting times and destroyed fibrinogen, FV, FVII, FX and FXIII.Lytic activity in whole blood (WB), albumin and saline was investigated by absorbance densitometry. Both agents induced haemolysis, platelet, platelet microparticle (PMP) and endothelial lysis at high concentrations. The lytic effect was neutralised by albumin and plasma proteins.Antithrombotic mechanisms were investigated in functional assays for activated protein C (APC), PC, protein S, antithrombin and FXa in normal plasma (NP). High concentration STS demonstrated anti-IIa, anti-Xa and anti-Va activity and potentiated the anticoagulant effects of APC. POL induced APC resistance.Fibrinolytic enzymes/inhibitors were measured by ELISA in WB and plasma, and plasminogen by a chromogenic assay in NP. Inhibitors of fibrinolysis were elevated at low concentrations of sclerosants. Fibrinolytic enzymes/inhibitors were destroyed by high concentration STS.Clot formation was assessed by thromboelasotometry in WB. Both agents induced strong clots at low concentrations, weak clots at mid-range and prevented clot formation at high concentrations. In turbidity measurements, neither agent had a lytic effect on cross-linked fibrin but STS destroyed non-cross-linked fibrin.Platelet and PMP counts were assessed by flow cytometry and platelet activation by ELISA for soluble markers and by flow cytometry for CD62p, CD63 and calcium. Platelet aggregation was assessed by light transmission and impedance aggregometry, and by flow cytometry for glycoprotein (GP)IIb/IIIa. At low concentrations, both agents induced platelet activation, released phosphatidylserine+ PMPs but inhibited aggregation by suppressing the activation of GPIIb/IIIa.In conclusion, detergent sclerosants interfered with coagulation, antithrombotic and fibrinolytic mechanisms. Both agents activated platelets, released procoagulant PMPs and potentiated prothrombotic and antifibrinolytic mechanisms at low concentrations. At high concentrations, both agents prevented clot formation. High concentration STS exhibited more anticoagulant activity than POL. Advisors/Committee Members: Joseph, Joanne Emily, Clinical School - St Vincent's Hospital, Faculty of Medicine, UNSW, Ma, David, Clinical School - St Vincent's Hospital, Faculty of Medicine, UNSW, Myers, Kenneth A, Epworth Hospital, Melbourne, VIC.

Subjects/Keywords: Detergents; Sclerosants; Sclerotherapy; Surfactants; Clotting factors; GPIIb/IIIa; Activated protein C; Annexin V; Phosphatidyl serine; Polidocanol; Coagulation; Clot formation; Thrombosis; Antithrombotic proteins; Plasminogen; Protein C; Protein S; Antithrombin; Fibrinolysis; Platelets; Microparticles; t-PA; PAI-1; Antiplasmin; Fibrinogen; Sodium tetradecyl sulphate; Fibrin; Thromboelastometry; Multiplate; Factor XIII; Albumin

Record DetailsSimilar RecordsGoogle PlusoneFacebookTwitterCiteULikeMendeleyreddit

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Parsi, K. (2011). Interaction of detergent sclerosants with coagulation, antithrombotic and fibrinolytic mecanisms. (Doctoral Dissertation). University of New South Wales. Retrieved from http://handle.unsw.edu.au/1959.4/50868 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:9762/SOURCE02?view=true

Chicago Manual of Style (16th Edition):

Parsi, Kurosh. “Interaction of detergent sclerosants with coagulation, antithrombotic and fibrinolytic mecanisms.” 2011. Doctoral Dissertation, University of New South Wales. Accessed September 24, 2020. http://handle.unsw.edu.au/1959.4/50868 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:9762/SOURCE02?view=true.

MLA Handbook (7th Edition):

Parsi, Kurosh. “Interaction of detergent sclerosants with coagulation, antithrombotic and fibrinolytic mecanisms.” 2011. Web. 24 Sep 2020.

Vancouver:

Parsi K. Interaction of detergent sclerosants with coagulation, antithrombotic and fibrinolytic mecanisms. [Internet] [Doctoral dissertation]. University of New South Wales; 2011. [cited 2020 Sep 24]. Available from: http://handle.unsw.edu.au/1959.4/50868 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:9762/SOURCE02?view=true.

Council of Science Editors:

Parsi K. Interaction of detergent sclerosants with coagulation, antithrombotic and fibrinolytic mecanisms. [Doctoral Dissertation]. University of New South Wales; 2011. Available from: http://handle.unsw.edu.au/1959.4/50868 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:9762/SOURCE02?view=true

.