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You searched for subject:(S pseudintermedius). Showing records 1 – 3 of 3 total matches.

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University of Edinburgh

1. Richards, Amy Claire. Functional characterisation of Staphylococcus pseudintermedius cell wall-associated proteins.

Degree: PhD, 2016, University of Edinburgh

Staphylococcus pseudintermedius is the major cause of the common canine skin disease, pyoderma, and is a zoonotic pathogen of humans. Multidrug resistant strains of S. pseudintermedius have emerged and are spreading globally leading to decreased therapeutic success. The development of novel therapeutics is hindered by the lack of understanding of critical host-pathogen interactions mediating S. pseudintermedius colonization and pathogenesis. For the major human pathogen Staphylococcus aureus, interactions with host fibrinogen play a fundamental role in pathogenesis. The aim of the current study was to genetically and functionally characterise 2 cell wall-associated proteins of S. pseudintermedius, SpsD and SpsL, which mediate binding to multiple host extracellular matrix proteins including fibrinogen and fibronectin. DNA sequencing of the A- (ligand binding) domains of spsD and spsL genes for 37 phylogenetically diverse isolates revealed a highly conserved sequence for SpsL (97.1% derived amino acid identity), in contrast to more extensive variation for SpsD (76.7% derived amino acid identity). Further, recombination events were identified to have contributed to the evolution of spsD diversity. Functional analysis with gene deletion mutants of S. pseudintermedius strain ED99, constructed in the current study, demonstrated that SpsL is a major cell wall-associated fibrinogen-binding protein with enhanced affinity for canine fibrinogen. Using recombinant chains of fibrinogen it was determined that SpsL binds to the α-chain of fibrinogen similar to clumping factor B (ClfB) of S. aureus. However, ELISA and surface plasmon resonance analyses of recombinant truncated derivatives of SpsL indicated that the predicted ligand-binding N2N3 subdomains of the A-domain of SpsL are not sufficient for high-affinity fibrinogen-binding suggesting that either additional domains or post-translational modifications are required for fibrinogen-binding. Furthermore, development of a murine skin infection model allowed an investigation of the contribution of SpsD and SpsL to pathogenesis revealing a role for SpsL in focal abscess pathology. Overall these studies have provided new insights into the diversity, function and therapeutic potential of S. pseudintermedius fibrinogen-binding proteins.

Subjects/Keywords: 616.9; SpsD; SpsL; ED99; Staphylococcus pseudintermedius; S. pseudintermedius; fibrinogen

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Richards, A. C. (2016). Functional characterisation of Staphylococcus pseudintermedius cell wall-associated proteins. (Doctoral Dissertation). University of Edinburgh. Retrieved from http://hdl.handle.net/1842/25715

Chicago Manual of Style (16th Edition):

Richards, Amy Claire. “Functional characterisation of Staphylococcus pseudintermedius cell wall-associated proteins.” 2016. Doctoral Dissertation, University of Edinburgh. Accessed January 23, 2021. http://hdl.handle.net/1842/25715.

MLA Handbook (7th Edition):

Richards, Amy Claire. “Functional characterisation of Staphylococcus pseudintermedius cell wall-associated proteins.” 2016. Web. 23 Jan 2021.

Vancouver:

Richards AC. Functional characterisation of Staphylococcus pseudintermedius cell wall-associated proteins. [Internet] [Doctoral dissertation]. University of Edinburgh; 2016. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/1842/25715.

Council of Science Editors:

Richards AC. Functional characterisation of Staphylococcus pseudintermedius cell wall-associated proteins. [Doctoral Dissertation]. University of Edinburgh; 2016. Available from: http://hdl.handle.net/1842/25715


Technical University of Lisbon

2. Mascarenhas, Diana Marisa Cardoso. Utilização de extracto de Staphylococcus pseudintermedius em testes alergológicos cutâneos no cão : um estudo preliminar.

Degree: 2014, Technical University of Lisbon

Dissertação de Mestrado Integrado em Medicina Veterinária

Nos cães com dermatite atópica, as infeções cutâneas recorrentes causadas pelo Staphylococcus pseudintermedius são extremamente comuns. Estudos prévios demonstraram que cães atópicos com piodermite recorrente têm títulos mais elevados de imunoglobulina E anti-Staphylococcus no soro. Os principais objetivos deste estudo foram definir qual a concentração ideal de extrato de alergénios de S. pseudintermedius a utilizar nos testes intradérmicos de cães atópicos e posteriormente determinar a frequência de hipersensibilidade cutânea ao S. pseudintermedius em cães atópicos com história de infeção cutânea recorrente. Para isso, realizaram-se testes intradérmicos, onde se testaram diferentes concentrações de extrato de um S. pseudintermedius resistente à meticilina (MRSP), estirpe 5819/10. Esta estirpe foi isolada de um cão com piodermite profunda e apresentava os seguintes genes de virulência: luk-I, se-int, siet, speta, ebpS e spsL. As concentrações utilizadas foram 2 μg/ml, 20 μg/ml e 200 μg/ml, assim como um controlo positivo (fosfato de histamina 0.001%) e um controlo negativo (PBS 0.9%). Foram incluídos neste estudo, vinte e três cães saudáveis e vinte e quatro cães atópicos com infeção cutânea recorrente por S. pseudintermedius, diagnosticados por citologia cutânea e/ou cultura bacteriana. Dos 23 cães saudáveis presentes neste estudo, 14 (61%) apresentaram reação positiva na concentração de 200 μg/ml. Assim sendo, é bastante provável que a concentração de 200 μg/ml seja irritante e por isso resulte em reações falso-positivas. A concentração de 20 μg/ml foi a concentração mais indicada para ser usada no diagnóstico de hipersensibilidade ao S. pseudintermedius em cães atópicos. Posteriormente, foram realizados testes intradérmicos em 24 canídeos atópicos com piodermite e/ou otite bacteriana recorrente com a concentração de 20 μg/ml; quatro destes animais apresentaram reações positivas nesta concentração, o que parecer indicar a existência de reações de hipersensibilidade imediata a componentes do S. pseudintermedius. No entanto, limitações que envolviam o extrato impediram a obtenção de resultados reprodutíveis e uniformes. Acreditamos que a imunoterapia específica para esta bactéria pode ser benéfica para o tratamento destes pacientes, o que torna fundamental o diagnóstico de hipersensibilidade ao S. pseudintermedius. Nesse sentido, deverão ser realizados mais estudos, de modo a obter um extrato fidedigno que permite alcançar consistência e reprodutibilidade em ambiente clínico e assim melhorar a qualidade dos resultados obtidos nos testes intradérmicos.

ABSTRACT - THE USE OF STAPHYLOCOCCUS PSEUDINTERMEDIUS EXTRACT IN SKIN ALLERGY TESTS ON THE DOG – A PRELIMINARY STUDY - In dogs with atopic dermatitis, recurrent skin infections caused by Staphylococcus pseudintermedius are extremely common. Previous studies demonstrated that atopic dogs with recurrent pyoderma have higher titters of anti-Staphylococcus immunoglobulin E. The main objectives of this study…

Advisors/Committee Members: Lourenço, Ana Mafalda Gonçalves Xavier Félix, Pomba, Maria Constança Matias Ferreira.

Subjects/Keywords: S. pseudintermedius; Extrato de alergénios; Infeção cutânea recorrente; Dermatite atópica canina; Allergens extracts; Recurrent pyoderma; Canine atopic dermatitis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Mascarenhas, D. M. C. (2014). Utilização de extracto de Staphylococcus pseudintermedius em testes alergológicos cutâneos no cão : um estudo preliminar. (Thesis). Technical University of Lisbon. Retrieved from http://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/7236

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Mascarenhas, Diana Marisa Cardoso. “Utilização de extracto de Staphylococcus pseudintermedius em testes alergológicos cutâneos no cão : um estudo preliminar.” 2014. Thesis, Technical University of Lisbon. Accessed January 23, 2021. http://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/7236.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Mascarenhas, Diana Marisa Cardoso. “Utilização de extracto de Staphylococcus pseudintermedius em testes alergológicos cutâneos no cão : um estudo preliminar.” 2014. Web. 23 Jan 2021.

Vancouver:

Mascarenhas DMC. Utilização de extracto de Staphylococcus pseudintermedius em testes alergológicos cutâneos no cão : um estudo preliminar. [Internet] [Thesis]. Technical University of Lisbon; 2014. [cited 2021 Jan 23]. Available from: http://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/7236.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mascarenhas DMC. Utilização de extracto de Staphylococcus pseudintermedius em testes alergológicos cutâneos no cão : um estudo preliminar. [Thesis]. Technical University of Lisbon; 2014. Available from: http://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/7236

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation


University of Helsinki

3. Aarnio, Sanna. Metisilliiniresistenttien Staphylococcus pseudintermedius –kantojen tunnistus ja tyypitys MALDI-TOF MS –menetelmällä.

Degree: Department of Equine and Small Animal Medicine; Helsingin yliopisto, Eläinlääketieteellinen tiedekunta, Kliinisen hevos- ja pieneläinlääketieteen osasto; Helsingfors universitet, Veterinärmedicinska fakulteten, Avdelningen för klinisk häst- och smådjursmedicin, 2017, University of Helsinki

Matriisiavusteinen massaspektrometria (Matrix Assisted Laser Desorption/Ionization Time Of Flight Mass Spectrometry, MALDI-TOF MS) on molekyylien massa-varaussuhteen mittaamiseen perustuva analyysimenetelmä. MALDI-TOF-massaspektrometriaa käytetään mikrobien lajintunnistukseen, mutta joillain mikrobilajeilla se saattaa soveltua myös lajitasoa tarkempaan analytiikkaan. MALDI-TOF-massaspektrometrian käytöstä Staphylococcus aureuksen metisilliiniresistenssiominaisuuden tunnistuksessa ja epi- demiologisessa tyypityksessä on saatu lupaavia tuloksia. Staphylococcus pseudintermediuksella vastaavaa tutkimusta ei ole aiemmin tehty. Tässä työssä selvitettiin MALDI-TOF-massaspektrometrian soveltuvuutta S. pseudintermedius -kantojen metisilliini-resistenssiominaisuuden tunnistukseen ja MRSP (metisilliinille resistenttien S. pseudintermedius) -kantojen epidemiologiseen tyypitykseen. Metisilliiniresistenssiominaisuuden tunnistuksessa tutkittiin 10 MRSP- kantaa ja 10 MSSP (metisilliinille sensitiivistä S. pseudinter-medius) -kantaa. MRSP:n epidemiologisessa tyypityksessä tutkittiin 15 MRSP-kantaa, joista kutakin tutkittua klonaalista kompleksia (CC45, CC71 ja CC258) edusti viisi kantaa. Massaspektrit mitattiin Bruker Microflex LT 3.1 -laitteella, ja spektrit käsiteltiin Flex-Analysis 3.4 -ohjelmalla. Erottavia huippuja etsittiin visuaaliseen spektritarkasteluun perustuvan kantakohtaisen huipputaulukoinnin avulla. MRSP-kannoista etsittiin paitsi erottavia huippuja yleisesti, myös kohdennetusti metisilliiniresistenssiominaisuuteen liittyvien PSM-mec- ja MecI-proteiinien massaa vastaavia huippuja. Visuaalisen analyysin lisäksi spektreistä luotiin kantakohtaiset kokoomaspektrit (Main Spectral Profile, MSP), joiden samankaltaisuutta tarkasteltiin FlexAnalysis-ohjelman klusterianalyysillä. MRSP- ja MSSP- kantojen spektrejä ei pystytty erottamaan toisistaan. MecI-proteiinin massaa vastaavia huippuja havaittiin sekä MRSP- että MSSP-kannoilla, ja PSM-mec-proteiinin massaa vastaava huippu havaittiin yhden MRSP-kannan isolaatilla. Klusterianalyysissä MRSP-kannat ryvästyivät sekaisin MSSP-kantojen kanssa. Kaikki klonaaliset MRSP-kompleksit pystyttiin erottamaan toisistaan huippujen 2047 +/-2 m/z ja 4290 +/-3 m/z esiintymisen perusteella. 2047 +/-2 m/z esiintyi kaikilla klonaalisen kompleksin CC258 kannoilla, mutta ei millään muilla kannoilla. 4290 +/-3 m/z esiintyi kaikilla CC71-kannoilla, mutta ei CC45-kannoilla. Eroja havaittiin myös useiden muiden huippujen esiintymisessä. Klusterianalyysissä klonaaliset kompleksit eivät erottuneet toisistaan. Tämän tutkimuksen perusteella MALDI-TOF saattaa soveltua joidenkin klonaalisten MRSP-kompleksien erotteluun, mutta menetelmän käytölle S. pseudintermediuksen metisilliiniresistenssin tunnistamisessa ei saatu puoltavaa näyttöä. Klonaalisten kompleksien erottelussa huippujen poiminta saattaa toimia paremmin kuin klusterianalyysi.

Subjects/Keywords: MRSP; S. pseudintermedius; MALDI-TOF MS; Matrix Assisted Laser Desorption/Ionization Time Of Flight Mass Spectrometry; Eläinlääketieteellinen kliininen diagnostiikka; Veterinärmedicinsk klinisk diagnostik; Veterinary Clinical Diagnostics; MRSP; S. pseudintermedius; MALDI-TOF MS; Matrix Assisted Laser Desorption/Ionization Time Of Flight Mass Spectrometry

Record DetailsSimilar RecordsGoogle PlusoneFacebookTwitterCiteULikeMendeleyreddit

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Aarnio, S. (2017). Metisilliiniresistenttien Staphylococcus pseudintermedius –kantojen tunnistus ja tyypitys MALDI-TOF MS –menetelmällä. (Thesis). University of Helsinki. Retrieved from http://hdl.handle.net/10138/231494

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Aarnio, Sanna. “Metisilliiniresistenttien Staphylococcus pseudintermedius –kantojen tunnistus ja tyypitys MALDI-TOF MS –menetelmällä.” 2017. Thesis, University of Helsinki. Accessed January 23, 2021. http://hdl.handle.net/10138/231494.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Aarnio, Sanna. “Metisilliiniresistenttien Staphylococcus pseudintermedius –kantojen tunnistus ja tyypitys MALDI-TOF MS –menetelmällä.” 2017. Web. 23 Jan 2021.

Vancouver:

Aarnio S. Metisilliiniresistenttien Staphylococcus pseudintermedius –kantojen tunnistus ja tyypitys MALDI-TOF MS –menetelmällä. [Internet] [Thesis]. University of Helsinki; 2017. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/10138/231494.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Aarnio S. Metisilliiniresistenttien Staphylococcus pseudintermedius –kantojen tunnistus ja tyypitys MALDI-TOF MS –menetelmällä. [Thesis]. University of Helsinki; 2017. Available from: http://hdl.handle.net/10138/231494

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

.