subject:(S Typhimurium)

Indian Institute of Science

1. Deobagkar-Lele, Mukta. Studies On The Mechanisms Involved In Thymic Atrophy During Salmonella Enterica Serovar Typhimurium Infection.

Degree: PhD, Faculty of Science, 2015, Indian Institute of Science

URL: http://etd.iisc.ac.in/handle/2005/2481

► T lymphocytes are an essential component of the adaptive immune response and are highly versatile in function. Each T cell has a unique T cell… (more)

▼ T lymphocytes are an essential component of the adaptive immune response and are highly versatile in function. Each T cell has a unique T cell receptor that can recognize an antigenic peptide in the context of the major his to compatibility complex (MHC) encoded molecules, thus offering a high degree of specificity to the immune response. T cells play a central role in the development of an effective host immune response and the quantitative and qualitative regulation of the T cell response is critical. T cells develop in the thymus, an important primary immune organ, where immature thymocytes undergo differentiation and maturation. Through the process of thymic differentiation, immature cluster of differentiation (CD)4-CD8- thymocytes progress to a CD4+CD8+ stage and are subjected to positive and negative selection to give rise to MHC restricted, single positive CD4+ or CD8+ naive T cells that emigrate from the thymus and populate the peripheral lymphocyte pool. Thymic atrophy is well known to occur naturally during the process of aging with thymocyte depletion and reduced thymic output. Along with age associated changes leading to atrophy, the thymus is exquisitely sensitive to starvation and several stresses. In addition, thymic atrophy is a characteristic feature during several viral, bacterial and parasitic infections. Egress of immature thymocytes, loss of thymic populations due to sensitivity to glucocorticoids and cytokine modulation, etc. have been variously proposed to be involved in this process. However there is limited understanding on the numerous mechanisms involved and the crosstalk between these diverse pathways. In this study, a model for thymic atrophy during acute Salmonella enterica serovar Typhimurium (S. typhimurium) infection was developed. S. typhimurium is a Gram negative bacterium that resides and grows in intracellular compartments within host cells. It causes gastroenteritis in humans but leads to typhoid like disease in mice, similar to that caused by S. typhi in humans. Initially, it was established that acute infection of C57BL/6 mice with 108 CFU S. typhimurium, via the oral, i.e. the physiological, route of infection leads to extensive depletion (8-10 fold) of thymocytes in an infection-dependent manner. Infected mice had higher CFU burden in the Peyer’s patches, spleen, liver, and mesenteric lymph node (MLN) as compared to the thymus. The thymic atrophy was dependent upon the infection caused by live S. typhimurium since oral feeding of mice even with higher doses (1010 CFU) of heat-killed bacteria did not lead to thymic atrophy. The susceptible populations in the thymus were identified by staining for expression of CD4 and CD8 on cell surface using specific monoclonal antibodies tagged to fluorophores, e.g. Fluorescein isothiocyanate (FITC) and phycoerythrin (PE), respectively. The double labelled samples were analyzed by flow cytometry. Interestingly, significant death of CD4+CD8+, the major population of thymocytes, but not single positive thymocytes or peripheral… Advisors/Committee Members: Nandi, Dipankar (advisor).

Subjects/Keywords: Salmonella Typhimurium Infection; Thymic Atrophy; Infections Diseases; Communicable Diseases; Salmonella Enterica Serovar Typhimurium; Thymic Atrophy - Glucocorticoids; S. typhimurium Infection; Glucocorticoids; Immunology

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APA (6^th Edition):

Deobagkar-Lele, M. (2015). Studies On The Mechanisms Involved In Thymic Atrophy During Salmonella Enterica Serovar Typhimurium Infection. (Doctoral Dissertation). Indian Institute of Science. Retrieved from http://etd.iisc.ac.in/handle/2005/2481

Chicago Manual of Style (16^th Edition):

Deobagkar-Lele, Mukta. “Studies On The Mechanisms Involved In Thymic Atrophy During Salmonella Enterica Serovar Typhimurium Infection.” 2015. Doctoral Dissertation, Indian Institute of Science. Accessed January 17, 2021. http://etd.iisc.ac.in/handle/2005/2481.

MLA Handbook (7^th Edition):

Deobagkar-Lele, Mukta. “Studies On The Mechanisms Involved In Thymic Atrophy During Salmonella Enterica Serovar Typhimurium Infection.” 2015. Web. 17 Jan 2021.

Vancouver:

Deobagkar-Lele M. Studies On The Mechanisms Involved In Thymic Atrophy During Salmonella Enterica Serovar Typhimurium Infection. [Internet] [Doctoral dissertation]. Indian Institute of Science; 2015. [cited 2021 Jan 17]. Available from: http://etd.iisc.ac.in/handle/2005/2481.

Council of Science Editors:

Deobagkar-Lele M. Studies On The Mechanisms Involved In Thymic Atrophy During Salmonella Enterica Serovar Typhimurium Infection. [Doctoral Dissertation]. Indian Institute of Science; 2015. Available from: http://etd.iisc.ac.in/handle/2005/2481

Indian Institute of Science

2. Majumdar, Shamik. Characterization of Thymocyte Subpopulations and Mechanistic Insights during Various Modes of Thymic Atrophy.

Degree: PhD, Faculty of Science, 2018, Indian Institute of Science

URL: http://etd.iisc.ac.in/handle/2005/4046

► T lymphocytes along with B lymphocytes constitute the adaptive immune system, which is critical for host defence. The progenitors of T cells arise in the… (more)

▼ T lymphocytes along with B lymphocytes constitute the adaptive immune system, which is critical for host defence. The progenitors of T cells arise in the bone marrow and the sequential development of T cells occurs in the thymus, a primary lymphoid organ. Developing thymocytes undergo selection and maturation processes in the thymus, post which only fully mature, non-self-reactive and immunocompetent T cells egress to the periphery. In the peripheral lymph nodes, T cells recognise antigenic peptides presented on major histocompatibility molecules by antigen presenting cells. Subsequently, T cell activation occurs to mount an effective immune response. Thymic atrophy i. e., reduction in the cellularity of the thymus, is well known to occur with ageing. It is also observed during other conditions such as malnutrition, trauma, infections, cancer chemotherapy, etc. As a consequence, the thymic output may be affected, leading to lower number of naïve T cells with limited T cell receptor diversity in the periphery. Although the process of thymic atrophy has been widely reported, a detailed characterization of the thymocyte subpopulations that are affected and the underlying mechanisms governing them has not been performed In this study, we utilized an acute and lethal infection model wherein C57BL/6 mice orally infected with Salmonella Typhimurium (S. Typhimurium) undergo extensive thymic atrophy. S. Typhimurium is a Gram-negative bacterium that causes gastroenteritis in humans but in mice leads to a Typhoid-like disease, similar to that caused by Salmonella Typhi (S. Typhi) in humans. Upon oral gavage of S. Typhimurium, severe thymic atrophy develops in mice. On the other hand, the cellularity of the mesenteric lymph node is much less affected during the course of infection. Using multicolour flow cytometry, we systematically studied the thymocyte subpopulations during S. Typhimurium-induced thymic atrophy. The major thymocyte subpopulations can be developmentally categorized on the basis of the cell surface expression of the T cell co-receptors, CD4 and CD8. The most immature cells lack CD4 and CD8 expression and are thus termed CD4-CD8- double negative (DN). These cells give rise to cells expressing both CD4 as well as CD8 i. e., double positive (DP) cells. Post selection and maturation, the DP cells either give rise to CD4+ single positive (SP) or CD8+ SP. During S. Typhimurium-induced thymic atrophy, in C57BL/6 mice where the thymic cellularity drops to about ~8-10 fold, we observed a great loss of DN and DP cells and to a lesser extent, loss of CD4+ SP cells. Using additional developmental and maturation markers, we subsequently studied the DN, DP and SP subsets. CD44 and CD25 expression aid in distinguishing the four stages of DN thymocyte development. The most immature CD44+CD25- DN (DN1) cells give rise to the CD44+CD25+ DN (DN2) cells, which in turn produces the CD44-CD25+ DN (DN3) cells, which further gives rise to the CD44-CD25- DN (DN4) cells. Infection resulted in a profound loss of DN2-DN4 cells, while… Advisors/Committee Members: Nandi, Dipankar (advisor).

Subjects/Keywords: Thymic Atrophy; Thymocyte; Salmonella Typhimurium Infection; S. Typhiumurium Infection; Biochemistry

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Majumdar, S. (2018). Characterization of Thymocyte Subpopulations and Mechanistic Insights during Various Modes of Thymic Atrophy. (Doctoral Dissertation). Indian Institute of Science. Retrieved from http://etd.iisc.ac.in/handle/2005/4046

Chicago Manual of Style (16^th Edition):

Majumdar, Shamik. “Characterization of Thymocyte Subpopulations and Mechanistic Insights during Various Modes of Thymic Atrophy.” 2018. Doctoral Dissertation, Indian Institute of Science. Accessed January 17, 2021. http://etd.iisc.ac.in/handle/2005/4046.

MLA Handbook (7^th Edition):

Majumdar, Shamik. “Characterization of Thymocyte Subpopulations and Mechanistic Insights during Various Modes of Thymic Atrophy.” 2018. Web. 17 Jan 2021.

Vancouver:

Majumdar S. Characterization of Thymocyte Subpopulations and Mechanistic Insights during Various Modes of Thymic Atrophy. [Internet] [Doctoral dissertation]. Indian Institute of Science; 2018. [cited 2021 Jan 17]. Available from: http://etd.iisc.ac.in/handle/2005/4046.

Council of Science Editors:

Majumdar S. Characterization of Thymocyte Subpopulations and Mechanistic Insights during Various Modes of Thymic Atrophy. [Doctoral Dissertation]. Indian Institute of Science; 2018. Available from: http://etd.iisc.ac.in/handle/2005/4046

3. Lamb, Favelle. Is there an increasing trend in domestic Salmonella infection in Sweden between 1997-2008.

Degree: Life Sciences, 2009, Södertörn University College

URL: http://urn.kb.se/resolve?urn=urn:nbn:se:sh:diva-2528

► An analysis of the recorded cases of Salmonella in Sweden indicates that there was no significant increase between 1997-2008, including or excluding outbreaks. The… (more)

Subjects/Keywords: Salmonella; domestic; S. Typhimurium; S. Enteritidis

10 more images…

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lamb, F. (2009). Is there an increasing trend in domestic Salmonella infection in Sweden between 1997-2008. (Thesis). Södertörn University College. Retrieved from http://urn.kb.se/resolve?urn=urn:nbn:se:sh:diva-2528

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lamb, Favelle. “Is there an increasing trend in domestic Salmonella infection in Sweden between 1997-2008.” 2009. Thesis, Södertörn University College. Accessed January 17, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:sh:diva-2528.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lamb, Favelle. “Is there an increasing trend in domestic Salmonella infection in Sweden between 1997-2008.” 2009. Web. 17 Jan 2021.

Vancouver:

Lamb F. Is there an increasing trend in domestic Salmonella infection in Sweden between 1997-2008. [Internet] [Thesis]. Södertörn University College; 2009. [cited 2021 Jan 17]. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:sh:diva-2528.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lamb F. Is there an increasing trend in domestic Salmonella infection in Sweden between 1997-2008. [Thesis]. Södertörn University College; 2009. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:sh:diva-2528

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

4. Letícia Ríspoli Coelho. Equivalência de métodos alternativos ao oficial para determinação de Salmonella Enteritidis e Typhimurium em amostras ambientais avícolas.

Degree: 2012, Federal University of Uberlândia

URL: http://www.bdtd.ufu.br//tde_busca/arquivo.php?codArquivo=4647

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A infecção de humanos por Salmonella, particularmente pelos sorovares Enteritidis e Typhimurium é uma preocupação para a saúde pública mundial. A análise deste patógeno nas… (more)

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A infecção de humanos por Salmonella, particularmente pelos sorovares Enteritidis e Typhimurium é uma preocupação para a saúde pública mundial. A análise deste patógeno nas fezes e no ambiente das aves é uma forma de monitorar a infecção nos lotes e verificar a necessidade de introdução de controles, havendo recomendação do Regulamento da Comissão Européia (CE) (n. 646/2007) de que os lotes de aves sejam analisados para a presença destes em fases anteriores ao abate. Diferentes metodologias podem ser utilizadas para a pesquisa desses microrganismos em amostras ambientais; entretanto, a maioria demanda um longo prazo para obtenção dos resultados. Assim, é desejável que outros métodos, mais rápidos e práticos tenham seu desempenho verificado para possível implantação na rotina. Objetivou-se, avaliar a equivalência entre os resultados obtidos pelos métodos: BAX, Meio Semi-sólido Rappaport-Vassiliadis Modificado (MSRV) (ISO 6579) e o método tradicional de referência oficial no Brasil (Portaria 126, MAPA) para pesquisa de S. Typhimurium e S. Enteritidis em amostras ambientais avícolas. Amostras de propé e fezes fortificadas com uma média de 100 a 1000 UFC/g de cada sorovar, e as mesmas amostras sem fortificação foram avaliadas pelos três métodos. Foram obtidos 504 diagnósticos e os mesmos analisados quanto à repetibilidade, reprodutibilidade, sensibilidade, especificidade e acurácia. Estes índices foram realizados individualmente e em relação ao método tradicional (índices relativos). Os resultados obtidos indicaram que os métodos analisados mostraram desempenho satisfatório e o teste de conformidade verificou correspondência entre os métodos alternativos e o método oficial, o que permite afirmar que as metodologias possuem desempenhos equivalentes.

The infection of humans with Salmonella, particularly by serovars Enteritidis and Typhimurium is a worldwide public health concern. The analysis of this pathogen in birds feces and environment is a way to monitoring the infection in batches and to verify the need of introduction of controls, with recommendation of European Commission Regulation (EC) (No. 646/2007) that lots of birds are being analyzed for the presence of these serovars in phases prior to slaughter. Different methodologies can be used to research these microrganisms in environmental samples, however, most of them demands a long time to obtain the results. Thus, it is desirable that other methods, faster and more practical have their performance verified for possible deployment in routine. The aim was to evaluate the equivalence between the results obtained by the methods: BAX , Half Semi-solid Rappaport- Vassiliadis Modified (MSRV) (ISO 6579) method and the traditional official reference in Brazil (Ordinance 126, MAPA) for Salmonella Typhimurium and S. Enteritidis in poultry environmental samples. Prope and feces samples fortified with an average of 100 to 1000 CFU/g of each serovar, and the same samples were evaluated without fortification by the three methods. Were obtained 504 diagnoses that were analyzed…

Advisors/Committee Members: Yara Cristina de Paiva Maia, Ana Cristina Gonçalves Pinto da Rocha, Daise Aparecida Rossi.

Subjects/Keywords: Sistema BAX; MSRV; Salmonella Enteritidis; S. Typhimurium; MEDICINA VETERINARIA; Aves domésticas; Salmonella Typhimurium; Aves domésticas - Doenças; BAX System

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Coelho, L. R. (2012). Equivalência de métodos alternativos ao oficial para determinação de Salmonella Enteritidis e Typhimurium em amostras ambientais avícolas. (Thesis). Federal University of Uberlândia. Retrieved from http://www.bdtd.ufu.br//tde_busca/arquivo.php?codArquivo=4647

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Coelho, Letícia Ríspoli. “Equivalência de métodos alternativos ao oficial para determinação de Salmonella Enteritidis e Typhimurium em amostras ambientais avícolas.” 2012. Thesis, Federal University of Uberlândia. Accessed January 17, 2021. http://www.bdtd.ufu.br//tde_busca/arquivo.php?codArquivo=4647.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Coelho, Letícia Ríspoli. “Equivalência de métodos alternativos ao oficial para determinação de Salmonella Enteritidis e Typhimurium em amostras ambientais avícolas.” 2012. Web. 17 Jan 2021.

Vancouver:

Coelho LR. Equivalência de métodos alternativos ao oficial para determinação de Salmonella Enteritidis e Typhimurium em amostras ambientais avícolas. [Internet] [Thesis]. Federal University of Uberlândia; 2012. [cited 2021 Jan 17]. Available from: http://www.bdtd.ufu.br//tde_busca/arquivo.php?codArquivo=4647.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Coelho LR. Equivalência de métodos alternativos ao oficial para determinação de Salmonella Enteritidis e Typhimurium em amostras ambientais avícolas. [Thesis]. Federal University of Uberlândia; 2012. Available from: http://www.bdtd.ufu.br//tde_busca/arquivo.php?codArquivo=4647

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

5. Massis, Liliana Moura. Imunogenicidade e potencial vacinal das flagelinas de Salmonella enterica sorovar Typhimurium.

Degree: PhD, Microbiologia, 2007, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/42/42132/tde-28012008-142626/ ;

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Flagelina, a subunidade estrutural dos flagelos bacterianos, pode ser empregada em estratégias vacinais como carregadora de epitopos antigênicos fusionados ou como adjuvante. Neste trabalho avaliamos… (more)

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Flagelina, a subunidade estrutural dos flagelos bacterianos, pode ser empregada em estratégias vacinais como carregadora de epitopos antigênicos fusionados ou como adjuvante. Neste trabalho avaliamos a imunogenicidade e o potencial vacinal das flagelinas Salmonella enterica sorovar Typhimurium em camundongos BALB/c após administração pelas vias oral ou intraperitoneal. Os resultados indicam que animais imunizados pela via oral com linhagens flageladas de S. Typhimurium não desenvolvem respostas de anticorpos, sistêmicos ou secretados, anti-flagelina, por outro lado, ativam respostas celulares específicas para as flagelinas em função da via de administração utilizada. Observamos ainda que as diferentes flagelinas testadas apresentam efeito adjuvante quando co-administradas pela via nasal com a fímbria CFA/I de ETEC. Em suma, os resultados apresentados contribuem para um melhor conhecimento sobre as propriedades imunológicas e adjuvantes das flagelinas de S. Typhimurium e agregam informações para o uso racional dessas proteínas em formulações vacinais.

Flagellin, the structural subunit of bacterial flagella, can be used in vaccine development as a fused antigenic epitope carrier or as an adjuvant. In this work we evaluated the immunogenicity and vaccine approach of S. Typhimurium flagellin in BALB/c mice after administration by oral or intraperitoneal route. Our results indicate that mice immunized by oral route with flagellated S. Typhimurium strains do not develop any anti-flagellin antibody response, be it serum or secreted. On the other hand, specific anti-flagellin cellular response was observed depending on the chosen route of immunization with S. Typhimurium attenuated strain. Furthermore, all flagellins tested proved to be efficient adjuvants when co-administrated with CFA/I fimbriae by nasal route. Together, these results contribute to a better understanding of the immunological and adjuvant properties of S. Typhimurium flagellins and also provide information on the rational use of these proteins in vaccine development.

Advisors/Committee Members: Almeida, Maria Elisabete Sbrogio de, Ferreira, Luis Carlos de Souza.

Subjects/Keywords: Adjuvantes; Adjuvants; Antígenos heterólogos; Attenuated S. Typhimurium; Flagelina; Flagellin; Heterologous antigens; Immune response; Mucosal vaccine.; Resposta imune; S. Typhimurium atenuada; Vacinas de mucosa.

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Massis, L. M. (2007). Imunogenicidade e potencial vacinal das flagelinas de Salmonella enterica sorovar Typhimurium. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/42/42132/tde-28012008-142626/ ;

Chicago Manual of Style (16^th Edition):

Massis, Liliana Moura. “Imunogenicidade e potencial vacinal das flagelinas de Salmonella enterica sorovar Typhimurium.” 2007. Doctoral Dissertation, University of São Paulo. Accessed January 17, 2021. http://www.teses.usp.br/teses/disponiveis/42/42132/tde-28012008-142626/ ;.

MLA Handbook (7^th Edition):

Massis, Liliana Moura. “Imunogenicidade e potencial vacinal das flagelinas de Salmonella enterica sorovar Typhimurium.” 2007. Web. 17 Jan 2021.

Vancouver:

Massis LM. Imunogenicidade e potencial vacinal das flagelinas de Salmonella enterica sorovar Typhimurium. [Internet] [Doctoral dissertation]. University of São Paulo; 2007. [cited 2021 Jan 17]. Available from: http://www.teses.usp.br/teses/disponiveis/42/42132/tde-28012008-142626/ ;.

Council of Science Editors:

Massis LM. Imunogenicidade e potencial vacinal das flagelinas de Salmonella enterica sorovar Typhimurium. [Doctoral Dissertation]. University of São Paulo; 2007. Available from: http://www.teses.usp.br/teses/disponiveis/42/42132/tde-28012008-142626/ ;

Indian Institute of Science

6. Arvindhan, G N. Typhoidal And Non-Typhoidal Salmonella Serovars - A Comparartive Study.

Degree: PhD, Faculty of Science, 2011, Indian Institute of Science

URL: http://etd.iisc.ac.in/handle/2005/1053

► Chapter Introduction Salmonellae are gram negative bacteria that cause gastroenteritis and entericfever. S. enterica is divided into seven phylogenetic groups, subspecies 1, 2,3a, 3b, and… (more)

▼ Chapter Introduction Salmonellae are gram negative bacteria that cause gastroenteritis and entericfever. S. enterica is divided into seven phylogenetic groups, subspecies 1, 2,3a, 3b, and 4, 6, 7. Subspecies1 includes 1,367 serovars, some of which are commonly isolated from infected birds and mammals. The other subspecies mainly colonize cold blooded animals. Salmonella typhimurium, Salmonella typhiandSalmonella enteritidis are some of the serovars, which belong to s.enterica species. S. typhimurium is one of the important causes for food poisoning in humans. It causes typhoid like fever in mice. In immuno compromised patients the infection is often fatal if it is not treated with antibiotics. Clinical features of food poisoning include abdominal pain, vomiting, nausea, abdominal cramps, dehydration etc. S. typhi causes typhoid fever in humans. No other host has been identified for this serovar. Main source of infection is contaminated food and water. No age is exempted but it is less common before2 years. Incubation period is 360 days. Clinical features include stepladder type fever, malaise, headache, hepato splenomegaly, coated tongue, Neutrogena etc. It may be fatal if untreated. Among the serovars of Salmonella infecting humans S. typhimurium and S. typhi are the most important. While S. typhimurium infects many host species including birds and mammals, S. typhi is single host adapted and infects only human. The single host adaptation of S. typhi presents it with the need for establishing are servoir of infection in the community which can serve as a source of fresh infection. Also the single host adaptation of S. typhi has made it a highly specialized pathogen which has evolved certain unique genes needed for human colonization at the same time has lost a set of genes which are needed for survival in other hosts and in the highly variable external environment. This has led to the accumulation of a vast number of pseudo genesin S. Typhi. A comparative study of the two serovars is useful in many ways. Due to varied host defense systems encountered by the two serovars owing to different niche of infection the bacterial counter defense mechanisms are also different. By focusing on the differences between genes involved in the bacterial defense of host immune response we can decipher the role played by various genes in combating the antibacterial host response. Chapter 2 The role of TolA and peptidoglycan modification in detergent resistance of pathogenic Salmonella The major Salmonella serovars that infect human are Salmonella enterica serovar Typhi (S.typhi) which cause systemic typhoid and Salmonella enterica serovar Typhimurium (S. typhimurium) which cause gastro enteritis. S. typhi resides in the gall bladder during chronic infection and S .typhimurium infects intestine .Thus both pathogens encounter high concentrations of bile and have developed mechanisms to counter it. The Tol Pal complex spanning the outermembrane and the inner cytoplasmic membrane … Advisors/Committee Members: Chakravorty, Dipshikha (advisor).

Subjects/Keywords: Typhoid Virus; Non-Typhoid Virus; Salmonella Pathogenesis; Salmonellosis; Salmonella Virulence; Salmonella - Resistance; DNA Vaccines; Salmonella Typhimurium; Salmonella Bacteria; Blood Borne Salmonella; Serovar Determination; Salmonella enterica; S. typhimurium; S. typhi; Microbiology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Arvindhan, G. N. (2011). Typhoidal And Non-Typhoidal Salmonella Serovars - A Comparartive Study. (Doctoral Dissertation). Indian Institute of Science. Retrieved from http://etd.iisc.ac.in/handle/2005/1053

Chicago Manual of Style (16^th Edition):

Arvindhan, G N. “Typhoidal And Non-Typhoidal Salmonella Serovars - A Comparartive Study.” 2011. Doctoral Dissertation, Indian Institute of Science. Accessed January 17, 2021. http://etd.iisc.ac.in/handle/2005/1053.

MLA Handbook (7^th Edition):

Arvindhan, G N. “Typhoidal And Non-Typhoidal Salmonella Serovars - A Comparartive Study.” 2011. Web. 17 Jan 2021.

Vancouver:

Arvindhan GN. Typhoidal And Non-Typhoidal Salmonella Serovars - A Comparartive Study. [Internet] [Doctoral dissertation]. Indian Institute of Science; 2011. [cited 2021 Jan 17]. Available from: http://etd.iisc.ac.in/handle/2005/1053.

Council of Science Editors:

Arvindhan GN. Typhoidal And Non-Typhoidal Salmonella Serovars - A Comparartive Study. [Doctoral Dissertation]. Indian Institute of Science; 2011. Available from: http://etd.iisc.ac.in/handle/2005/1053

7. Targant, Hayette. L’îlot de multirésistance aux antibiotiques, Salmonella Genomic Island 1 (SGI1) : variabilité, diffusion inter - espèces et implication dans la virulence : The multidrug resistance island, Salmonella Genomic Island 1 (SGI1) : variability, inter-species diffusion and implication in virulence.

Degree: Docteur es, Sciences de la vie et de la santé, 2010, Université Claude Bernard – Lyon I

URL: http://www.theses.fr/2010LYO10157

► Les salmonelles sont l’une des premières causes d’infections bactériennes d’origine alimentaire. Depuis le début des années 1990, l’isolement de salmonelles multirésistantes aux antibiotiques a considérablement… (more)

▼ Les salmonelles sont l’une des premières causes d’infections bactériennes d’origine alimentaire. Depuis le début des années 1990, l’isolement de salmonelles multirésistantes aux antibiotiques a considérablement accru avec l’émergence des souches épidémiques Salmonella Typhimurium DT104 qui sont, pour la majorité, résistantes à l’ampicilline, le chloramphénicol, la streptomycine, les sulfamides et les tétracyclines. Les gènes codant ces résistances sont regroupés sur un intégron complexe de classe 1 nommé In104, localisé lui-même sur un îlot génomique de 43 kb désigné Salmonella Genomic Island 1 (SGI1). Depuis sa première identification chez S. Typhimurium DT104, SGI1 a été identifié à travers le monde chez plusieurs sérovars de Salmonella, et plus récemment chez Proteus mirabilis. Chez ces souches, la multirésistance aux antibiotiques est liée, soit à l’îlot SGI1 dans sa forme initialement décrite, soit à des variants de SGI1 correspondant à la structure initiale de SGI1 comportant des modifications au niveau de l’intégron complexe In104. L’îlot génomique Salmonella Genomic Island 1 (SGI1) représente une préoccupation importante car le phénotype de multirésistance qu’il confère aux souches bactériennes est souvent responsable d’échecs thérapeutiques pouvant entrainer des complications importantes, voire la mort. Dans ce contexte, le travail de thèse a été centré sur l’enjeu sanitaire majeur représenté par cette diffusion épidémique du clone S. Typhimurium au cours des années 1990 chez l’homme et les bovins. Les travaux entrepris dans le cadre de la thèse ont eu, en premier lieu, l’objectif d’apprécier l’évolution moléculaire de SGI1 dix années après l’émergence de ces souches en élevage bovin, puis d’évaluer la diffusion de SGI1 chez des souches naturelles appartenant à d’autres genres bactériens que Salmonella. Il a ainsi été dressé un bilan de la multirésistance aux antibiotiques chez les souches de S. Typhimurium isolées de bovins malades en France de 2002 à 2007 et une recherche de la présence de SGI1, chez d’autres espèces bactériennes que Salmonella, et par sondage à partir de leurs phénotypes de résistance, a été mise en œuvre. Les résultats obtenus ont indiqué un faible pouvoir évolutif de SGI1 qui semble en contradiction avec les capacités moléculaires majeures de recombinaison et de transfert démontrées tant in vitro qu’in vivo. Les études menées ont toutefois permis la première description d’un nouveau variant, nommé SGI1-T, qui résulte d’une recombinaison intramoléculaire. Le deuxième grand objectif de la thèse a été de contribuer à une meilleure connaissance du rôle que pourrait avoir SGI1 dans la virulence bactérienne. Une première stratégie de modélisation expérimentale (salmonellose systémique murine) a ainsi été conduite, qui visait à comparer le pouvoir virulent in vivo de souches isogéniques ne se distinguant que par la présence ou l’absence de SGI1. Une seconde approche a été également menée, qui a consisté en une évaluation du rôle de SGI1 dans la formation de biofilms, l’organisation en biofilms… Advisors/Committee Members: Madec, Jean-Yves (thesis director).

Subjects/Keywords: Salmonella; SGI1; Multirésistance aux antibiotiques; Virulence; Biofilm; Quorum sensing; S. Typhimurium DT104; Salmonella; SGI1; Multidrug-resistance; Antimicrobial; Virulence; Biofilm; Quorum sensing; S. Typhimurium DT104

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Targant, H. (2010). L’îlot de multirésistance aux antibiotiques, Salmonella Genomic Island 1 (SGI1) : variabilité, diffusion inter - espèces et implication dans la virulence : The multidrug resistance island, Salmonella Genomic Island 1 (SGI1) : variability, inter-species diffusion and implication in virulence. (Doctoral Dissertation). Université Claude Bernard – Lyon I. Retrieved from http://www.theses.fr/2010LYO10157

Chicago Manual of Style (16^th Edition):

Targant, Hayette. “L’îlot de multirésistance aux antibiotiques, Salmonella Genomic Island 1 (SGI1) : variabilité, diffusion inter - espèces et implication dans la virulence : The multidrug resistance island, Salmonella Genomic Island 1 (SGI1) : variability, inter-species diffusion and implication in virulence.” 2010. Doctoral Dissertation, Université Claude Bernard – Lyon I. Accessed January 17, 2021. http://www.theses.fr/2010LYO10157.

MLA Handbook (7^th Edition):

Targant, Hayette. “L’îlot de multirésistance aux antibiotiques, Salmonella Genomic Island 1 (SGI1) : variabilité, diffusion inter - espèces et implication dans la virulence : The multidrug resistance island, Salmonella Genomic Island 1 (SGI1) : variability, inter-species diffusion and implication in virulence.” 2010. Web. 17 Jan 2021.

Vancouver:

Targant H. L’îlot de multirésistance aux antibiotiques, Salmonella Genomic Island 1 (SGI1) : variabilité, diffusion inter - espèces et implication dans la virulence : The multidrug resistance island, Salmonella Genomic Island 1 (SGI1) : variability, inter-species diffusion and implication in virulence. [Internet] [Doctoral dissertation]. Université Claude Bernard – Lyon I; 2010. [cited 2021 Jan 17]. Available from: http://www.theses.fr/2010LYO10157.

Council of Science Editors:

Targant H. L’îlot de multirésistance aux antibiotiques, Salmonella Genomic Island 1 (SGI1) : variabilité, diffusion inter - espèces et implication dans la virulence : The multidrug resistance island, Salmonella Genomic Island 1 (SGI1) : variability, inter-species diffusion and implication in virulence. [Doctoral Dissertation]. Université Claude Bernard – Lyon I; 2010. Available from: http://www.theses.fr/2010LYO10157

Université de Sherbrooke

8. Tremblay, Sarah. Étude de la réponse antimicrobienne intestinale dans un état sain et dans le vieillissement : nouveaux rôles des sels biliaires: Regulation of intestinal antimicrobial response in health and aging : new roles of bile acids.

Degree: 2018, Université de Sherbrooke

URL: http://hdl.handle.net/11143/12161

► Abstract : Intestinal antimicrobial response plays a critical role in gut homeostasis and in defense against pathogenic infections. One of their major mechanisms is the… (more)

▼ Abstract : Intestinal antimicrobial response plays a critical role in gut homeostasis and in defense against pathogenic infections. One of their major mechanisms is the synthesis and secretion of antimicrobial peptides and proteins (AMPP). Bile acids metabolism is shown to be disrupted in many pathologies such as inflammatory bowel diseases, bacterial infections, obesity and aging. The first objective of this study is designed to determine wether bile acids are implicated in the regulation of intestinal AMPP expression using a 0,1% CDCA supplemented diet. We demontrate that CDCA increased the base-line synthesis of intestinal AMPP in vivo; it also had an effect on the mucosal immune cell populations and on goblet cell fonction, by affecting the number of cells and the relative expression of Muc2. Mice fed with CDCA showed a limited spread of systemic S. typhimurium and promoted a significantly faster clearance of C. rodentium. This effects of CDCA do not seem to be dependent on the activation of STAT3 or activation of the bile acid receptors (TGR5, FXR, PXR, VDR). The second objective was to caracterize the antimicrobial response and the antimicrobial profile in aging using 104 weeks old mice. We observed degeneration of the villi architecture in the ileum of most aged animals suggesting defects on the integrity of the epithelial barrier. No detectable immune cell infiltration was observed. Increased numbers of intermediate cells were found in the ileal crypts and along the villi, suggesting inefficient terminal differentiation of the secretory cell lineage in the aged ileum. AMPP expression is also altered in aged animals. We also observed goblet cell hyperplasia but no differences in Muc2 transcript level. Electron microscopy confirmed cellular degeneration of the villi and the presence of atypical secretion granules in the Paneth cells of aged mice. Moreover, sequencing of V4 region of 16s gene, we documented differences in the composition of the intestinal microbiota. Aged mice lost the presence of Clostridiales. We also demonstrated that the CDCA-mediated mechanism for induction of the intestinal AMPP is still functional in the aged ileum. In conclusion we demonstrated for the first time a new role for bile acid in the regulation of intestinal antibacterial peptide synthesis. We also showed that significant morphological and transcriptional changes accompany the aging of the terminal ileum and we described for the first time the AMPP expression profile in aged mice. Advisors/Committee Members: Menendez, Alfredo (advisor).

Subjects/Keywords: CDCA; AMPP; Défense antimicrobienne; S. typhimurium; C. rodentium; Vieillissement; Antimicrobial defense; Aging

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Tremblay, S. (2018). Étude de la réponse antimicrobienne intestinale dans un état sain et dans le vieillissement : nouveaux rôles des sels biliaires: Regulation of intestinal antimicrobial response in health and aging : new roles of bile acids. (Doctoral Dissertation). Université de Sherbrooke. Retrieved from http://hdl.handle.net/11143/12161

Chicago Manual of Style (16^th Edition):

Tremblay, Sarah. “Étude de la réponse antimicrobienne intestinale dans un état sain et dans le vieillissement : nouveaux rôles des sels biliaires: Regulation of intestinal antimicrobial response in health and aging : new roles of bile acids.” 2018. Doctoral Dissertation, Université de Sherbrooke. Accessed January 17, 2021. http://hdl.handle.net/11143/12161.

MLA Handbook (7^th Edition):

Tremblay, Sarah. “Étude de la réponse antimicrobienne intestinale dans un état sain et dans le vieillissement : nouveaux rôles des sels biliaires: Regulation of intestinal antimicrobial response in health and aging : new roles of bile acids.” 2018. Web. 17 Jan 2021.

Vancouver:

Tremblay S. Étude de la réponse antimicrobienne intestinale dans un état sain et dans le vieillissement : nouveaux rôles des sels biliaires: Regulation of intestinal antimicrobial response in health and aging : new roles of bile acids. [Internet] [Doctoral dissertation]. Université de Sherbrooke; 2018. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/11143/12161.

Council of Science Editors:

Tremblay S. Étude de la réponse antimicrobienne intestinale dans un état sain et dans le vieillissement : nouveaux rôles des sels biliaires: Regulation of intestinal antimicrobial response in health and aging : new roles of bile acids. [Doctoral Dissertation]. Université de Sherbrooke; 2018. Available from: http://hdl.handle.net/11143/12161

University of Georgia

9. Soni, Vivek. Genetic approach to understanding the behavior of Salmonella in meat and poultry system.

Degree: 2014, University of Georgia

URL: http://hdl.handle.net/10724/24288

► The genome projects have supplied a rich source of genetic data regarding chromosome structure and gene transfer. In this study, we utilized a genomic approach… (more)

Subjects/Keywords: Salmonella Typhimurium; virulence factors; S. enterica 4,[5],12:i:-; Antibiotic Resistance; Prebiotic; BiomossTM

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APA (6^th Edition):

Soni, V. (2014). Genetic approach to understanding the behavior of Salmonella in meat and poultry system. (Thesis). University of Georgia. Retrieved from http://hdl.handle.net/10724/24288

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Soni, Vivek. “Genetic approach to understanding the behavior of Salmonella in meat and poultry system.” 2014. Thesis, University of Georgia. Accessed January 17, 2021. http://hdl.handle.net/10724/24288.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Soni, Vivek. “Genetic approach to understanding the behavior of Salmonella in meat and poultry system.” 2014. Web. 17 Jan 2021.

Vancouver:

Soni V. Genetic approach to understanding the behavior of Salmonella in meat and poultry system. [Internet] [Thesis]. University of Georgia; 2014. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/10724/24288.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Soni V. Genetic approach to understanding the behavior of Salmonella in meat and poultry system. [Thesis]. University of Georgia; 2014. Available from: http://hdl.handle.net/10724/24288

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Georgia

10. Afari, George Kwabena. Efficacy of neutral electrolyzed water in inactivating Escherichia coli O157:H7 and Salmonella Typhimurium DT 104 on fresh produce items under simulated food service conditions.

Degree: 2014, University of Georgia

URL: http://hdl.handle.net/10724/30818

► Lettuce and tomatoes were inoculated with 100µl of a 5-strain cocktail of Escherichia coli O157:H7 or Salmonella Typhimurium DT 104 and stored at 40C overnight.… (more)

Subjects/Keywords: Neutral electrolyzed water; fresh produce; ultrasound; E. coli O157:H7; S. Typhimurium DT 104.

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APA (6^th Edition):

Afari, G. K. (2014). Efficacy of neutral electrolyzed water in inactivating Escherichia coli O157:H7 and Salmonella Typhimurium DT 104 on fresh produce items under simulated food service conditions. (Thesis). University of Georgia. Retrieved from http://hdl.handle.net/10724/30818

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Afari, George Kwabena. “Efficacy of neutral electrolyzed water in inactivating Escherichia coli O157:H7 and Salmonella Typhimurium DT 104 on fresh produce items under simulated food service conditions.” 2014. Thesis, University of Georgia. Accessed January 17, 2021. http://hdl.handle.net/10724/30818.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Afari, George Kwabena. “Efficacy of neutral electrolyzed water in inactivating Escherichia coli O157:H7 and Salmonella Typhimurium DT 104 on fresh produce items under simulated food service conditions.” 2014. Web. 17 Jan 2021.

Vancouver:

Afari GK. Efficacy of neutral electrolyzed water in inactivating Escherichia coli O157:H7 and Salmonella Typhimurium DT 104 on fresh produce items under simulated food service conditions. [Internet] [Thesis]. University of Georgia; 2014. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/10724/30818.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Afari GK. Efficacy of neutral electrolyzed water in inactivating Escherichia coli O157:H7 and Salmonella Typhimurium DT 104 on fresh produce items under simulated food service conditions. [Thesis]. University of Georgia; 2014. Available from: http://hdl.handle.net/10724/30818

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Indian Institute of Science

11. Deka, Geeta. Structural and Functional Studies on Pyridoxal Kinase and Pyridoxal 5′-phosphate Dependent Enzymes.

Degree: PhD, Faculty of Science, 2018, Indian Institute of Science

URL: http://etd.iisc.ac.in/handle/2005/3779

► Most of the chemical reactions of living cells are catalyzed by protein enzymes. These enzymes are very efficient and display a high degree of specificity… (more)

▼ Most of the chemical reactions of living cells are catalyzed by protein enzymes. These enzymes are very efficient and display a high degree of specificity with respect to the reaction catalyzed. Cellular activities depend critically on the precise three-dimensional structure and function of thousands of enzymes. Many enzymes require binding of metal ions or small organic molecules for their function. The organic molecules that are indispensible components of catalysis by proteins are called coenzymes. Pyridoxal 5ʹ-phosphate (PLP) is a versatile coenzyme found in all living cells. PLP-dependent enzymes play a key role in the function of most of the enzymes catalyzing reactions in the metabolic pathways of amino acid synthesis and degradation. The enzyme pyridoxal kinase serves to make available the co-enzyme PLP to apo-PLP dependent enzymes. Because of their key role in cellular function and their medical importance, the structure and function of PLP-dependent enzymes have been extensively investigated. In the past decade, detailed investigations on the structure and function of several PLP-dependent enzymes have been carried out in our laboratory. The enzymes studied are B. subtilis serinehydroxymethyl transferase (SHMT), S. typhimurium acetylornithine aminotransferase (AcOAT), S. typhimurium and E. coli diaminopropionate ammonia lyase (DAPAL), S. typhimurium D-serine dehydratase (DSD), S. typhimurium D-cysteine desulfhydrase (DCyD) and S. typhimurium arginine decarboxylase (ArgD). The extensive studies conducted on PLP-dependent enzymes in our laboratory during the past decade has not only resulted in deeper understanding of their structure and function but also raised several new questions regarding substrate recognition, reaction specificity, role of active site residues in the catalytic reaction, mechanism of catalysis and potential applications of these enzymes. This thesis is an attempt to answer some of these questions. The thesis also presents the structure and function of a new protein, Salmonella typhimurium pyridoxal kinase, the enzyme that provides PLP for PLP-dependent enzymes. Single crystal X-ray diffraction technique is the most powerful tool currently available for the elucidation of the three-dimensional structures of proteins and other biological macromolecules and for revealing the relationship between their structure and function. X-ray diffraction studies have provided in depth understanding of the topology of secondary structural elements in the three-dimensional structures of proteins, the hierarchical organization of protein domains, structural basis for the substrate specificity of enzymes, intricate details of mechanisms of enzyme catalyzed reactions, allosteric regulation of enzyme activity, mechanisms of feed-back inhibition, structural basis of protein stability, symmetry of oligomeric proteins and their possible biological implications and a myriad of other biochemical and biophysical properties of proteins. The work reported in this thesis is primarily based on X-ray diffraction… Advisors/Committee Members: Murthy, M R N (advisor).

Subjects/Keywords: Pyridoxal Kinase; Pyridoxal 5'-Phosphate Enzymes; Salmonella Typhimurium Arginine Decarboxylase; Escherechia Coil; Latent Tuberculosis Infection; E. coli Diaminopropionate Ammonia Lyase; S. typhimurium Arginine Decarboxylase; Diaminopropionate Ammonia Lyase; Molecular Biophysics

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APA (6^th Edition):

Deka, G. (2018). Structural and Functional Studies on Pyridoxal Kinase and Pyridoxal 5′-phosphate Dependent Enzymes. (Doctoral Dissertation). Indian Institute of Science. Retrieved from http://etd.iisc.ac.in/handle/2005/3779

Chicago Manual of Style (16^th Edition):

Deka, Geeta. “Structural and Functional Studies on Pyridoxal Kinase and Pyridoxal 5′-phosphate Dependent Enzymes.” 2018. Doctoral Dissertation, Indian Institute of Science. Accessed January 17, 2021. http://etd.iisc.ac.in/handle/2005/3779.

MLA Handbook (7^th Edition):

Deka, Geeta. “Structural and Functional Studies on Pyridoxal Kinase and Pyridoxal 5′-phosphate Dependent Enzymes.” 2018. Web. 17 Jan 2021.

Vancouver:

Deka G. Structural and Functional Studies on Pyridoxal Kinase and Pyridoxal 5′-phosphate Dependent Enzymes. [Internet] [Doctoral dissertation]. Indian Institute of Science; 2018. [cited 2021 Jan 17]. Available from: http://etd.iisc.ac.in/handle/2005/3779.

Council of Science Editors:

Deka G. Structural and Functional Studies on Pyridoxal Kinase and Pyridoxal 5′-phosphate Dependent Enzymes. [Doctoral Dissertation]. Indian Institute of Science; 2018. Available from: http://etd.iisc.ac.in/handle/2005/3779

Indian Institute of Science

12. Iyer, Namrata. Racemases in Salmonella : Insights into the Dexterity of the Pathogen.

Degree: PhD, Faculty of Science, 2018, Indian Institute of Science

URL: http://etd.iisc.ac.in/handle/2005/3183

► Chapter -I Introduction Salmonella is a pathogen well-known for its ability to infect a wide variety of hosts and causes disease ranging from mild gastroenteritis… (more)

▼ Chapter -I Introduction Salmonella is a pathogen well-known for its ability to infect a wide variety of hosts and causes disease ranging from mild gastroenteritis to typhoid fever. During infection, it is exposed to a myriad of conditions; from the aquatic environment, the gut lumen to the phagolysosome. The success of Salmonella as a pathogen lies in its ability to sense each of these environments and adapt itself for survival and proliferation accordingly. This is done mainly via the action of specific two-component systems (TCSs) which sense cues specific to each of these niches and trigger the appropriate transcriptional reprogramming. This reprogramming is best studied for the genes directly known to be involved in virulence. In the case of Salmonella, most of these genes are a part of specific clusters, acquired through horizontal gene transfer, known as Salmonella Pathogenicity Islands (SPIs). Of the various SPIs, the two most important are SPI-1 and SPI-2. SPI-1 is classically involved in orchestrating bacterial invasion of non-phagocytic cells in the gut, allowing the pathogen to invade the host. Furthermore, its role is well characterized in the classic inflammation associated with gastroenteritis. On the other hand, SPI-2 is specialized for survival within the harsh intracellular environment of host cells such as macrophages and epithelial cells. Other important virulence determinants include motility, chemotaxis as well as adhesins. The transcription of these virulence genes is under tight regulation and responsive to environmental conditions. Many small molecules such as short chain fatty acids, pp(p)Gpp, bile and acyl homoserine lactones among others are known to be potent regulators of virulence in Salmonella. Furthermore, the metabolic products of the normal flora in the gut also affect its virulence. Thus the metabolic status, of both the host as well as the pathogen, plays an important role in determining the outcome of the infection. Many metabolic enzymes and their products are now known to directly or indirectly affect virulence gene expression. In this study, we explore one such class of metabolic enzymes viz amino acid racemases. They catalyze the chiral conversion of L-amino acids to D-amino acids and vice versa. We have studied the biochemical properties of two such non-canonical racemases as well as their role in bacterial survival and pathogenesis. Chapter-II Identification and characterization of putative aspartate racemases in Salmonella Amino acid racemases, such as alanine and glutamate racemases, are ubiquitously found in all bacteria and they play an essential role in cell wall biosynthesis. Recently it has been found, that bacteria possess other amino acid racemases which produce non-canonical D-amino acids. These D-amino acids, upon secretion, further orchestrate various phenotypes such as cell wall remodeling and biofilm dispersal. In this study, we have explored the ability of Salmonella to produce such non-canonical D-amino acids. The genome of S. Typhimurium possesses genes… Advisors/Committee Members: Chakravortty, Dipshikha (advisor).

Subjects/Keywords: Salmonella Typhimurium; Salmonella Pathogenicity Islands (SPIs); Amino Acid Racemases; Salmonella Pathogenesis; D-Amino Acids; Salmonella Racemases; Aspartate Racemases; Bacterial Virulence; Salmonella Virulence; Putative Aspartate Racemases; Salmonella Infection; S. Typhimurium; Mirobiology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Iyer, N. (2018). Racemases in Salmonella : Insights into the Dexterity of the Pathogen. (Doctoral Dissertation). Indian Institute of Science. Retrieved from http://etd.iisc.ac.in/handle/2005/3183

Chicago Manual of Style (16^th Edition):

Iyer, Namrata. “Racemases in Salmonella : Insights into the Dexterity of the Pathogen.” 2018. Doctoral Dissertation, Indian Institute of Science. Accessed January 17, 2021. http://etd.iisc.ac.in/handle/2005/3183.

MLA Handbook (7^th Edition):

Iyer, Namrata. “Racemases in Salmonella : Insights into the Dexterity of the Pathogen.” 2018. Web. 17 Jan 2021.

Vancouver:

Iyer N. Racemases in Salmonella : Insights into the Dexterity of the Pathogen. [Internet] [Doctoral dissertation]. Indian Institute of Science; 2018. [cited 2021 Jan 17]. Available from: http://etd.iisc.ac.in/handle/2005/3183.

Council of Science Editors:

Iyer N. Racemases in Salmonella : Insights into the Dexterity of the Pathogen. [Doctoral Dissertation]. Indian Institute of Science; 2018. Available from: http://etd.iisc.ac.in/handle/2005/3183

13. Lupolova, Nadejda. Bacterial host attribution and bioinformatic characterisation of enteric bacteria Salmonella enterica and Escherichia coli from different hosts and environments.

Degree: PhD, 2019, University of Edinburgh

URL: http://hdl.handle.net/1842/35835

► With the advent of relatively low cost whole genome sequencing (WGS), it is now possible to obtain sequences from large numbers of bacterial strains and… (more)

▼ With the advent of relatively low cost whole genome sequencing (WGS), it is now possible to obtain sequences from large numbers of bacterial strains and interrogate their core and accessory genomes in relation to associated metadata. While there are some bacterial species with preferred hosts, especially in terms of disease, there has been no real systematic genomic investigation of host and niche specificity of 'generalist' bacteria, i.e., those that can be isolated from multiple hosts and environments. The main aim of this research was to determine if host and/or niche-specific proteins can be identified for 'multi-host adapted' bacteria such as E. coli and Salmonella Typhimurium (STm) in order to predict the 'origin' of a strain and its zoonotic potential from its sequence. Two datasets of 'multi-host' bacteria were analysed: 1,203 STm isolates from 4 hosts (avian, bovine, human and swine) and E. coli from 6 hosts (avian, bovine, canine, environmental, human and swine). Based on classical core genome analysis such as core phylogeny, multilocus sequence typing and phylo-grouping, no strong correlations with host were identified. The accessory genome was also investigated for host-based associations, and accessory host associated proteins (HAP) were identified for each of the bacteria/ host groups. These proteins were used to build a machine learning (ML) classifier - support vector machine (SVM) - to predict the isolation host of the bacterial isolates. The majority of the isolates from both species were predicted correctly with prediction accuracy ranging from 67% to 90%. For both bacterial species the most challenging were bovine and swine host groups as these two had many features in common. The approach allowed not only prediction of host based on WGS but also an assessment of how much the genome of particular isolates resembled the features of the genomes of the same species isolated from other hosts. This allowed 'generalist' and 'specialist' strains from each host group to be estimated as well as the sequences that indicate successful transmission potential between hosts. This work also showed that diverse collections of E. coli or STm can be used as a baseline for prediction and quantification of zoonotic potential as was demonstrated with E. coli O157 and Salmonella serovar Typhi. Overall this part of the research indicated marked host restriction for both STm and E. coli, with only limited isolate subsets exhibiting host promiscuity based on predicted protein content. ML can be successfully applied to interrogate source attribution of bacterial isolates and has the capacity to predict zoonotic potential. Using the same ML approach, another question was asked about how similar are the known zoonotic pathogens. When studied apart, E. coli O157 can be classified further into human and bovine isolates with only a small proportion of bovine isolates predicted as 'human', pointing to the specific cattle strains that are potentially a more serious threat to human health. This approach was tested with 2…

Subjects/Keywords: virulence determinants; bacteriophage regions; plasmids; whole genome sequencing; Escherichia coli; Salmonella Typhimurium; host specific proteins; niche-specific proteins; S. Typhimurium; machine learning; zoonotic potential prediction

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lupolova, N. (2019). Bacterial host attribution and bioinformatic characterisation of enteric bacteria Salmonella enterica and Escherichia coli from different hosts and environments. (Doctoral Dissertation). University of Edinburgh. Retrieved from http://hdl.handle.net/1842/35835

Chicago Manual of Style (16^th Edition):

Lupolova, Nadejda. “Bacterial host attribution and bioinformatic characterisation of enteric bacteria Salmonella enterica and Escherichia coli from different hosts and environments.” 2019. Doctoral Dissertation, University of Edinburgh. Accessed January 17, 2021. http://hdl.handle.net/1842/35835.

MLA Handbook (7^th Edition):

Lupolova, Nadejda. “Bacterial host attribution and bioinformatic characterisation of enteric bacteria Salmonella enterica and Escherichia coli from different hosts and environments.” 2019. Web. 17 Jan 2021.

Vancouver:

Lupolova N. Bacterial host attribution and bioinformatic characterisation of enteric bacteria Salmonella enterica and Escherichia coli from different hosts and environments. [Internet] [Doctoral dissertation]. University of Edinburgh; 2019. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/1842/35835.

Council of Science Editors:

Lupolova N. Bacterial host attribution and bioinformatic characterisation of enteric bacteria Salmonella enterica and Escherichia coli from different hosts and environments. [Doctoral Dissertation]. University of Edinburgh; 2019. Available from: http://hdl.handle.net/1842/35835

14. ΑΧΛΑΔΗ -ΑΛΕΙΦΕΡΗ, ΠΑΡΑΣΚΕΥΗ. ΕΠΙΒΙΩΣΗ ΤΟΥ ΒΑΚΤΗΡΙΟΦΑΓΟΥ 1 ΤΟΥ ΣΧΗΜΑΤΟΣ ΛΥΣΙΤΥΠΙΑΣ ΤΗΣ SPACE TYPHIMURIUM ΚΑΙ ΤΟΥ ΣΤΕΛΕΧΟΥΣ 36 ΤΗΣ ΣΑΛΜΟΝΕΛΛΑΣ ΑΥΤΗΣ ΣΤΟ ΧΩΜΑ.

Degree: 1989, Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); National and Kapodistrian University of Athens

URL: http://hdl.handle.net/10442/hedi/5191

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ΣΤΗΝ ΕΡΓΑΣΙΑ ΑΥΤΗ ΜΕΛΕΤΗΘΗΚΕ Η ΕΠΙΒΙΩΣΗ ΤΟΥ ΦΑΓΟΥ 1 ΤΟΥ ΣΧΗΜΑΤΟΣ ΛΥΣΙΤΥΠΙΑΣ ΤΗΣ S. TYPHIMURIUM ΣΕ ΤΕΣΣΕΡΑ ΕΙΔΗ ΑΠΟΣΤΕΙΡΩΜΕΝΟΥ ΧΩΜΑΤΟΣ ΚΑΙ ΣΕ ΤΡΕΙΣ ΔΙΑΦΟΡΕΤΙΚΕΣ ΘΕΡΜΟΚΡΑΣΙΕΣ… (more)

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ΣΤΗΝ ΕΡΓΑΣΙΑ ΑΥΤΗ ΜΕΛΕΤΗΘΗΚΕ Η ΕΠΙΒΙΩΣΗ ΤΟΥ ΦΑΓΟΥ 1 ΤΟΥ ΣΧΗΜΑΤΟΣ ΛΥΣΙΤΥΠΙΑΣ ΤΗΣ S. TYPHIMURIUM ΣΕ ΤΕΣΣΕΡΑ ΕΙΔΗ ΑΠΟΣΤΕΙΡΩΜΕΝΟΥ ΧΩΜΑΤΟΣ ΚΑΙ ΣΕ ΤΡΕΙΣ ΔΙΑΦΟΡΕΤΙΚΕΣ ΘΕΡΜΟΚΡΑΣΙΕΣ ΚΑΙ Η ΕΠΙΒΙΩΣΗ ΤΟΥ ΦΑΓΟΥ 1 ΚΑΙ ΤΟΥ ΣΤΕΛΕΧΟΥΣ 36 ΤΗΣ S. TYPHIMURIUM, ΟΤΑΝ ΕΜΒΟΛΙΑΖΟΝΤΑΙ ΜΑΖΙ ΣΤΑ ΤΕΣΣΕΡΑ ΕΙΔΗ ΧΩΜΑΤΟΣ ΚΑΙ ΣΤΙΣ ΙΔΙΕΣ ΘΕΡΜΟΚΡΑΣΙΕΣ. ΣΚΟΠΟΣ ΗΤΑΝ ΑΦ' ΕΝΟΣ ΜΕΝ Η ΜΕΛΕΤΗ ΤΗΣ ΕΠΙΒΙΩΣΗΣ ΤΩΝ ΦΑΓΩΝ ΤΗΣ ΣΑΛΜΟΝΕΛΛΑΣ ΣΤΟ ΕΔΑΦΟΣ ΚΑΙ ΤΩΝ ΠΑΡΑΓΟΝΤΩΝ ΠΟΥ ΤΗΝ ΕΠΗΡΕΑΖΟΥΝ, ΑΦ' ΕΤΕΡΟΥ ΓΙΑ ΘΕΜΑΤΑ ΠΡΑΚΤΙΚΟΥ ΕΝΔΙΑΦΕΡΟΝΤΟΣ ΓΙΑΤΙ ΤΟ ΠΕΙΡΑΜΑ ΑΥΤΟ ΕΞΟΜΟΙΩΝΕΙ ΤΙΣ ΣΥΝΘΗΚΕΣ ΔΙΑΒΙΩΣΗΣ ΤΩΝ ΕΝΤΕΡ ΟΙΩΝ ΣΤΟ ΧΩΜΑ. ΟΣΟΝ ΑΦΟΡΑ ΣΤΟ ΔΕΥΤΕΡΟ ΠΕΙΡΑΜΑ, ΛΑΜΒΑΝΟΝΤΑΣ ΥΠ'ΟΨΙΝ ΤΗΝ ΣΗΜΑΣΙΑ ΓΙΑ ΤΗΝ ΔΗΜΟΣΙΑ ΥΓΕΙΑ ΤΗΣ ΕΠΙΒΙΩΣΗΣ ΤΗΣ S. TYPHIMURIUM ΣΤΟ ΧΩΜΑ, ΚΡΙΘΗΚΕ ΣΚΟΠΙΜΟ ΝΑ ΓΙΝΕΙ ΣΧΕΤΙΚΗ ΕΡΕΥΝΑ ΓΙΑ ΝΑ ΜΕΛΕΤΗΘΕΙ Η ΑΛΛΗΛΕΞΑΡΤΗΣΗ ΚΑΙ ΑΛΛΗΛΕΠΙΔΡΑΣΗ ΤΩΝ ΣΑΛΜΟΝΕΛΛΩΝ ΚΑΙ ΤΩΝ ΒΑΚΤΗΡΙΟΦΑΓΩΝ ΤΟΥΣ ΚΑΙ ΤΩΝ ΠΑΡΑΓΟΝΤΩΝ ΠΟΥ ΕΠΗΡΕΑΖΟΥΝ ΤΗΝ ΚΟΙΝΗ ΤΟΥΣ ΕΠΙΒΙΩΣΗ. ΤΑ ΑΠΟΤΕΛΕΣΜΑΤΑ ΕΔΕΙΞΑΝ ΟΤΙ Η ΘΕΡΜΟΚΡΑΣΙΑ ΕΙΝΑΙ ΣΗΜΑΝΤΙΚΟΣ ΠΑΡΑΓΟΝΤΑΣ ΓΙΑ ΤΗΝ ΕΠΙΒΙΩΣΗ ΤΟΥ ΦΑΓΟΥ ΕΝΩ ΤΟ ΕΙΔΟΣ ΤΟΥ ΧΩΜΑΤΟΣ ΔΕ ΦΑΙΝΕΤΑΙ ΝΑ ΕΧΕΙ ΑΝΕΞΑΡΤΗΤΗ ΣΧΕΣΗ ΜΕ ΤΗΝ ΕΠΙΒΙΩΣΗ ΚΑΙ Η ΕΠΙΒΙΩΣΗ ΤΗΣ S. TYPHIMURIUM ΔΕΝ ΕΠΗΡΕΑΖΕΤΑΙ ΣΕ ΣΤΑΤΙΣΤΙΚΑ ΣΗΜΑΝΤΙΚΟ ΒΑΘΜΟ ΟΥΤΕ ΑΠΟ ΤΗΝ ΘΕΡΜΟΚΡΑΣΙΑ ΟΥΤΕ ΑΠΟ ΤΟ ΕΙΔΟΣ ΤΟΥ ΧΩΜΑΤΟΣ.

IN THIS WORK THE SURVIVAL OF BACTERIOPHAGE 1 OF THE S. TYPHIMURIUM PHAGE TYPING SCHEME WAS STUDIED IN FOUR TYPES OF STERILE SOIL AND THREE DIFFERENT TEMPERATURES. THE SURVIVAL OF BACTERIOPHAGE 1 AND S. TYPHIMURIUM 36 STRAIN WHEN INOCULATED TOGETHER IN THE SAME TYPES OF SOIL AND THE SAME TEMPERATURES WAS ALSO STUDIED IN A SECOND EXPERIMENT. FROM THE RESULTS OF THIS WORK AND THEIR STATISTIC ANALYSIS IT CAN BE CONCLUDED THAT THE TEMPERATURES OF INCUBATION IS A VERY IMPORTANT FACTOR FOR THE SURVIVAL OF BACTERIOPHAGE WHILE THE TYPE OF SOIL DOES NOT SEEM TO HAVE AN INDEPENDENT RELATION TO THE SURVIVAL WHILE UNDER THESE CONDITIONS NEITHER TEMPERATURE OF INCUBATION NOR THE TYPE OF SOIL AFFECTED IN A STATISTICALLY CONSIDERABLE WAY THE SURVIVAL OF S. TYPHIMURIUM. THE SIGNIFICANCE OF THESE FINDINGS FOR ECOLOGICAL, EPIDEMIOLOGICAL AND PUBLIC HEALTH PURPOSES ARE ALSO DISCUSSED.

Subjects/Keywords: BACTERIOPHAGE 1 OF THE S. TYPHIMURIUM PHAGE TYPING SCHEME; S. TYPHIMURIUM 36 STRAIN; Soil; Survival; Επιβίωση; ΣΤΕΛΕΧΟΣ 36 ΤΗΣ S. TYPHIMURIUM; ΦΑΓΟΣ 1 ΤΟΥ ΣΧΗΜΑΤΟΣ ΛΥΣΙΤΥΠΙΑΣ ΤΗΣ S. TYPHIMURIUM; ΧΩΜΑ

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

ΑΧΛΑΔΗ -ΑΛΕΙΦΕΡΗ, �. (1989). ΕΠΙΒΙΩΣΗ ΤΟΥ ΒΑΚΤΗΡΙΟΦΑΓΟΥ 1 ΤΟΥ ΣΧΗΜΑΤΟΣ ΛΥΣΙΤΥΠΙΑΣ ΤΗΣ SPACE TYPHIMURIUM ΚΑΙ ΤΟΥ ΣΤΕΛΕΧΟΥΣ 36 ΤΗΣ ΣΑΛΜΟΝΕΛΛΑΣ ΑΥΤΗΣ ΣΤΟ ΧΩΜΑ. (Thesis). Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); National and Kapodistrian University of Athens. Retrieved from http://hdl.handle.net/10442/hedi/5191

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

ΑΧΛΑΔΗ -ΑΛΕΙΦΕΡΗ, ΠΑΡΑΣΚΕΥΗ. “ΕΠΙΒΙΩΣΗ ΤΟΥ ΒΑΚΤΗΡΙΟΦΑΓΟΥ 1 ΤΟΥ ΣΧΗΜΑΤΟΣ ΛΥΣΙΤΥΠΙΑΣ ΤΗΣ SPACE TYPHIMURIUM ΚΑΙ ΤΟΥ ΣΤΕΛΕΧΟΥΣ 36 ΤΗΣ ΣΑΛΜΟΝΕΛΛΑΣ ΑΥΤΗΣ ΣΤΟ ΧΩΜΑ.” 1989. Thesis, Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); National and Kapodistrian University of Athens. Accessed January 17, 2021. http://hdl.handle.net/10442/hedi/5191.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

ΑΧΛΑΔΗ -ΑΛΕΙΦΕΡΗ, ΠΑΡΑΣΚΕΥΗ. “ΕΠΙΒΙΩΣΗ ΤΟΥ ΒΑΚΤΗΡΙΟΦΑΓΟΥ 1 ΤΟΥ ΣΧΗΜΑΤΟΣ ΛΥΣΙΤΥΠΙΑΣ ΤΗΣ SPACE TYPHIMURIUM ΚΑΙ ΤΟΥ ΣΤΕΛΕΧΟΥΣ 36 ΤΗΣ ΣΑΛΜΟΝΕΛΛΑΣ ΑΥΤΗΣ ΣΤΟ ΧΩΜΑ.” 1989. Web. 17 Jan 2021.

Vancouver:

ΑΧΛΑΔΗ -ΑΛΕΙΦΕΡΗ �. ΕΠΙΒΙΩΣΗ ΤΟΥ ΒΑΚΤΗΡΙΟΦΑΓΟΥ 1 ΤΟΥ ΣΧΗΜΑΤΟΣ ΛΥΣΙΤΥΠΙΑΣ ΤΗΣ SPACE TYPHIMURIUM ΚΑΙ ΤΟΥ ΣΤΕΛΕΧΟΥΣ 36 ΤΗΣ ΣΑΛΜΟΝΕΛΛΑΣ ΑΥΤΗΣ ΣΤΟ ΧΩΜΑ. [Internet] [Thesis]. Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); National and Kapodistrian University of Athens; 1989. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/10442/hedi/5191.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

ΑΧΛΑΔΗ -ΑΛΕΙΦΕΡΗ �. ΕΠΙΒΙΩΣΗ ΤΟΥ ΒΑΚΤΗΡΙΟΦΑΓΟΥ 1 ΤΟΥ ΣΧΗΜΑΤΟΣ ΛΥΣΙΤΥΠΙΑΣ ΤΗΣ SPACE TYPHIMURIUM ΚΑΙ ΤΟΥ ΣΤΕΛΕΧΟΥΣ 36 ΤΗΣ ΣΑΛΜΟΝΕΛΛΑΣ ΑΥΤΗΣ ΣΤΟ ΧΩΜΑ. [Thesis]. Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); National and Kapodistrian University of Athens; 1989. Available from: http://hdl.handle.net/10442/hedi/5191

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Georgia

15. Vereen, Ethell. Defining linkages between land-use, Salmonella, and Campylobacter in the Satilla River Basin (Georgia, USA).

Degree: 2014, University of Georgia

URL: http://hdl.handle.net/10724/27025

► From August 2007 to August 2009 a study of the Satilla River Basin (SRB), a mixed-use rural watershed located in the coastal plain of southern… (more)

▼ From August 2007 to August 2009 a study of the Satilla River Basin (SRB), a mixed-use rural watershed located in the coastal plain of southern Georgia (USA), was conducted to provide an understanding of landuse and existing water quality in these important surface waters. Watersheds were sampled that: 1) represent agricultural areas receiving poultry litter application, 2) agricultural areas with poultry houses and receiving poultry litter application, 3) reference areas with little or no agricultural activity, 4) a small watershed receiving direct discharge from a wastewater treatment plant (WWTP); and 5) larger watersheds on the main channel of the Satilla and Seventeen Mile River’s. Landuse differences were responsible water quality and nutrient variability. Eighty-three of the dissolved oxygen (DO) samples (35.78%, N=232) were below the Georgia Environmental Protection Division DO minimum, indicating that the SRB did not support its designated recreational use. Salmonella and Campylobacter were detected at all 13 sites monitored, and their prevalence varied by location (i.e., upstream vs. downstream of the WWTP). Pathogens were more frequently present among the sites with agricultural landuse and poultry production. Salmonella were recovered from 43% (129 of 299) of all samples. Salmonella serogroups identified were serogroup C (59%), B (14%), D (14%), and E (13%). Salmonella enterica serotype Montevideo (23%), Braenderup (14%) and Saint Paul (13%) were the most frequently detected of 16 different serotypes identified. Campylobacter were recovered from 62% (96 of 156) of all samples. C. jejuni (30%) was the most prevalent species detected followed by C. lari (22%), C. upsaliensis (11%), and C. coli (3%). Enterococci were reasonably predictive of Salmonella and Campylobacter presence; however 62% (80/129) of Salmonella were detected when E. coli were below EPA standards and 73% (70/96) of Campylobacter were detected when fecal coliforms were below EPA standards. These results highlight the drawback of the current fecal indicator system as proxies for enteric bacteria such as Salmonella and Campylobacter, which were highly prevalent in this area. This study indicates that these pathogens likely arise from numerous sources, including humans, and that there may be risk of human exposure through recreational contact.

Subjects/Keywords: Salmonella; Campylobacter; C. jejuni; C. coli; C. lari; C. upsaliensis; S. Montevideo; S. Braenderup; S. Typhimurium; S. Javiana; S. Newport; S. Muenchen; Salmonella serotype; Salmonella serovar; antimicrobial susceptibility; Satilla River Basin; Satilla

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Vereen, E. (2014). Defining linkages between land-use, Salmonella, and Campylobacter in the Satilla River Basin (Georgia, USA). (Thesis). University of Georgia. Retrieved from http://hdl.handle.net/10724/27025

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Vereen, Ethell. “Defining linkages between land-use, Salmonella, and Campylobacter in the Satilla River Basin (Georgia, USA).” 2014. Thesis, University of Georgia. Accessed January 17, 2021. http://hdl.handle.net/10724/27025.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Vereen, Ethell. “Defining linkages between land-use, Salmonella, and Campylobacter in the Satilla River Basin (Georgia, USA).” 2014. Web. 17 Jan 2021.

Vancouver:

Vereen E. Defining linkages between land-use, Salmonella, and Campylobacter in the Satilla River Basin (Georgia, USA). [Internet] [Thesis]. University of Georgia; 2014. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/10724/27025.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Vereen E. Defining linkages between land-use, Salmonella, and Campylobacter in the Satilla River Basin (Georgia, USA). [Thesis]. University of Georgia; 2014. Available from: http://hdl.handle.net/10724/27025

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Mississippi State University

16. Zhao, Li. EXPRESSION, PURIFICATION, AND ANTIMICROBIAL ACTIVITY OF AVIAN BETA-DEFENSIN-2, -6, AND -12.

Degree: MS, Veterinary Medicine, College of, 2011, Mississippi State University

URL: http://sun.library.msstate.edu/ETD-db/theses/available/etd-04012011-144211/ ;

► Total RNA was extracted from chicken oviduct epithelial cells. Avian Beta-defensin (AvBD)-2, -6, and -12 cDNAs were amplified… (more)

▼ Total RNA was extracted from chicken oviduct epithelial cells. Avian Beta-defensin (AvBD)-2, -6, and -12 cDNAs were amplified by reverse transcription-PCR and cloned into pRSET A, a protein expression vector. The hexa-histidine-tagged AvBD peptides were expressed in Escherichia coli (E. coli) BL21(DE3)plysS and affinity-purified. The antimicrobial activities of the recombinant AvBDs against E. coli, Salmonella <a name="OLE_LINK9"></a><a name="OLE_LINK8"></a>enterica serovar Typhimurium (S. typhimurium), and Staphylococcus aureus (S. aureus) were determined. At 8, 16 and 32 <a name="OLE_LINK17">µg/ml</a>, all three rAvBDs killed and inhibited the growth of E. coli, S. typhimurium, and S. aureus. The killing of rAvBD-2, -6, and -12 against stationary phase E. coli and S. aureus was pH dependent in the range investigated. In addition, the killing-curves showed that rAvBDs exerted their antimicrobial function within 30 minutes of treatment, suggesting the fast killing mechanisms of rAvBDs. Advisors/Committee Members: R. Hartford Bailey (chair).

Subjects/Keywords: colony-counting assay; S. aureus; E. coli; Antimicrobial activity; S. enterica serovar Typhimurium; Killing kinetics; Growth inhibitory activities; Avian â-Defensin; AvBD

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Zhao, L. (2011). EXPRESSION, PURIFICATION, AND ANTIMICROBIAL ACTIVITY OF AVIAN BETA-DEFENSIN-2, -6, AND -12. (Masters Thesis). Mississippi State University. Retrieved from http://sun.library.msstate.edu/ETD-db/theses/available/etd-04012011-144211/ ;

Chicago Manual of Style (16^th Edition):

Zhao, Li. “EXPRESSION, PURIFICATION, AND ANTIMICROBIAL ACTIVITY OF AVIAN BETA-DEFENSIN-2, -6, AND -12.” 2011. Masters Thesis, Mississippi State University. Accessed January 17, 2021. http://sun.library.msstate.edu/ETD-db/theses/available/etd-04012011-144211/ ;.

MLA Handbook (7^th Edition):

Zhao, Li. “EXPRESSION, PURIFICATION, AND ANTIMICROBIAL ACTIVITY OF AVIAN BETA-DEFENSIN-2, -6, AND -12.” 2011. Web. 17 Jan 2021.

Vancouver:

Zhao L. EXPRESSION, PURIFICATION, AND ANTIMICROBIAL ACTIVITY OF AVIAN BETA-DEFENSIN-2, -6, AND -12. [Internet] [Masters thesis]. Mississippi State University; 2011. [cited 2021 Jan 17]. Available from: http://sun.library.msstate.edu/ETD-db/theses/available/etd-04012011-144211/ ;.

Council of Science Editors:

Zhao L. EXPRESSION, PURIFICATION, AND ANTIMICROBIAL ACTIVITY OF AVIAN BETA-DEFENSIN-2, -6, AND -12. [Masters Thesis]. Mississippi State University; 2011. Available from: http://sun.library.msstate.edu/ETD-db/theses/available/etd-04012011-144211/ ;

Indian Institute of Science

17. Chittori, Sagar. Metabolic Adaptation For Utilization Of Short-Chain Fatty Acids In Salmonella Typhimurium : Structural And Functional Studies On 2-methylcitrate Synthase, Acetate And Propionate Kinases.

Degree: PhD, Faculty of Science, 2013, Indian Institute of Science

URL: http://etd.iisc.ac.in/handle/2005/2209

► Three-dimensional structures of proteins provide insights into the mechanisms of macromolecular assembly, enzyme catalysis and mode of activation, substrate-specificity, ligand-binding properties, stability and dynamical features.… (more)

▼ Three-dimensional structures of proteins provide insights into the mechanisms of macromolecular assembly, enzyme catalysis and mode of activation, substrate-specificity, ligand-binding properties, stability and dynamical features. X-ray crystallography has become the method of choice in structural biology due to the remarkable methodological advances made in the generation of intense X-ray beams with very low divergence, cryocooling methods to prolong useful life of irradiated crystals, sensitive methods of Xray diffraction data collection, automated and fast methods for data processing, advances and automation in methods of computational crystallography, comparative analysis of macromolecular structures along with parallel advances in biochemical and molecular biology methods that allow production of the desired biomolecule in quantities sufficient for X-ray diffraction studies. Advances in molecular biology techniques and genomic data have helped in identifying metabolic pathways responsible for metabolism of short-chain fatty acids (SCFAs). The primary objective of this thesis is application of crystallographic techniques for understanding the structure and function of enzymes involved in the metabolism of SCFAs in S. typhimurium. Pathways chosen for the present study are (i) propionate degradation to pyruvate and succinate by 2-methylcitrate pathway involving gene products of the prp operon, (ii) acetate activation to acetyl-CoA by AckA-Pta pathway involving gene products of the ack-pta operon, (iii) threonine degradation to propionate involving gene products of the tdc operon, (iv) 1,2-propanediol (1,2-PD) degradation to propionate involving gene products of the pdu operon. These metabolic pathways utilize a large number of enzymes with diverse catalytic mechanisms. The main objectives of the work include structural and functional studies on 2-methycitrate synthase (PrpC), acetate kinase (AckA), propionate kinase isoforms (PduW and TdcD) and propanol dehydrogenase (PduQ) from S. typhimurium. In the present work, these proteins were cloned, expressed, purified and characterized. The purified proteins were crystallized using standard methods. The crystals were placed in an X-ray beam and diffraction data were collected and used for the elucidation of structure of the proteins. The structures were subjected to rigorous comparative analysis and the results were complemented with suitable biochemical and biophysical experiments. The thesis begins with a review of the current literature on SCFAs metabolism in bacteria, emphasizing studies carried out on S. typhimurium and the closely related E. coli as well as organisms for which the structure of a homologue has been determined (Chapter 1). Metabolic pathways involving acetate utilization by activation to acetyl- CoA, propionate degradation to pyruvate and succinate, anaerobic degradation of Lthreonine to propionate and, 1,2-PD degradation to propionate are described in this chapter. Common experimental and computational methods used during the course of… Advisors/Committee Members: Murthy, M R N (advisor).

Subjects/Keywords: Short-chain Fatty Acid (SCFA); Salmonella typhimurium; Macromolecular Crystallography; Enzyme Kinetics; Computational Crystallography; S. typhimurium; 2-methylcitrate Synthase (PrpC); Propionate Kinase (PduW); Propanol Dehydrogenase (PduQ); Threonine Deaminase (TdcB); Acetate Kinase (AckA); TdcD; Biochemistry

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Chittori, S. (2013). Metabolic Adaptation For Utilization Of Short-Chain Fatty Acids In Salmonella Typhimurium : Structural And Functional Studies On 2-methylcitrate Synthase, Acetate And Propionate Kinases. (Doctoral Dissertation). Indian Institute of Science. Retrieved from http://etd.iisc.ac.in/handle/2005/2209

Chicago Manual of Style (16^th Edition):

Chittori, Sagar. “Metabolic Adaptation For Utilization Of Short-Chain Fatty Acids In Salmonella Typhimurium : Structural And Functional Studies On 2-methylcitrate Synthase, Acetate And Propionate Kinases.” 2013. Doctoral Dissertation, Indian Institute of Science. Accessed January 17, 2021. http://etd.iisc.ac.in/handle/2005/2209.

MLA Handbook (7^th Edition):

Chittori, Sagar. “Metabolic Adaptation For Utilization Of Short-Chain Fatty Acids In Salmonella Typhimurium : Structural And Functional Studies On 2-methylcitrate Synthase, Acetate And Propionate Kinases.” 2013. Web. 17 Jan 2021.

Vancouver:

Chittori S. Metabolic Adaptation For Utilization Of Short-Chain Fatty Acids In Salmonella Typhimurium : Structural And Functional Studies On 2-methylcitrate Synthase, Acetate And Propionate Kinases. [Internet] [Doctoral dissertation]. Indian Institute of Science; 2013. [cited 2021 Jan 17]. Available from: http://etd.iisc.ac.in/handle/2005/2209.

Council of Science Editors:

Chittori S. Metabolic Adaptation For Utilization Of Short-Chain Fatty Acids In Salmonella Typhimurium : Structural And Functional Studies On 2-methylcitrate Synthase, Acetate And Propionate Kinases. [Doctoral Dissertation]. Indian Institute of Science; 2013. Available from: http://etd.iisc.ac.in/handle/2005/2209

Université Laval

18. Brousseau, Jean-Philippe. Évaluation chez le porcelet de l'effet des probiotiques « Pediococcus acidilactici » et « Saccharomyces cerevisiae ssp. boulardii » sur le microbiote intestinal et sur les réponses innées et acquises lors d'une infection à « Salmonella Typhimurium DT 104 ».

Degree: 2013, Université Laval

URL: http://hdl.handle.net/20.500.11794/24266

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Tableau d’honneur de la Faculté des études supérieures et postdoctorales, 2013-2014.

Les suppléments antibiotiques dans l'alimentation animale sont sévèrement critiqués. Les probiotiques sont une alternative… (more)

▼

Tableau d’honneur de la Faculté des études supérieures et postdoctorales, 2013-2014.

Les suppléments antibiotiques dans l'alimentation animale sont sévèrement critiqués. Les probiotiques sont une alternative prometteuse, mais la caractérisation de leurs effets demeure nécessaire. Ce mémoire décrit l'influence de Pediococcus acidilactici (PA) et Saccharomyces cerevisiae ssp. boulardii (SCB) sur 1) le microbiote intestinal avant et après le sevrage et, 2) l'immunité et la colonisation intestinale lors d'une infection à Salmonella Typhimurium DT104. Nos résultats montrent que suite au sevrage PA module le microbiote iléal de façon similaire aux antibiotiques tandis que SCB influence le microbiote colique. De plus, SCB seul ou avec PA module certaines populations de cellules immunitaires du sang avant et après l'infection à S. Typhimurium. Cependant, aucun effet n'a été observé sur les autres paramètres évalués. Même si nous approfondissons la compréhension entourant les effets de PA et SCB sur l'hôte, d'autres études sont nécessaires pour optimiser l'utilisation des probiotiques comme alternative aux suppléments d'antibiotiques dans l'élevage.

Antibiotics as growth promoters in pig feed are severely criticized. Probiotics are a promising alternative, but characterization of their effects on the intestinal microbiota and immunity is still necessary. In this study, the influences of Pediococcus acidilactici (PA) and Saccharomyces cerevisiae ssp. boulardii (SCB) on 1) intestinal microbiota before and after weaning and, on 2) immunity and intestinal colonization during a Salmonella Typhimurium DT104 infection were evaluated. Our results show that following weaning PA modulated ileal microbiota similarly to in-feed antibiotic while SCB influenced the colonic microbiota. Moreover, SCB alone or with PA modulate some immune blood cell populations before and after the S. Typhimurium infection. However, no effects were observed on the other parameters assessed. Although we deepened the understanding surrounding the effects of PA and SCB on the host, further studies are needed to fully optimize the use of probiotics as alternatives to antibiotic supplements in animal husbandry.

Advisors/Committee Members: Lessard, Martin, Roy, Denis.

Subjects/Keywords: S 405 UL 2013; Pediococcus acidilactici – Effets physiologiques; Saccharomyces cerevisiæ – Effets physiologiques; Salmonella typhimurium – Lutte contre; Porcelets – Maladies – Aspect nutritionnel; Porcelets – Maladies – Traitement; Intestins – Microbiologie

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APA (6^th Edition):

Brousseau, J. (2013). Évaluation chez le porcelet de l'effet des probiotiques « Pediococcus acidilactici » et « Saccharomyces cerevisiae ssp. boulardii » sur le microbiote intestinal et sur les réponses innées et acquises lors d'une infection à « Salmonella Typhimurium DT 104 ». (Thesis). Université Laval. Retrieved from http://hdl.handle.net/20.500.11794/24266

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Brousseau, Jean-Philippe. “Évaluation chez le porcelet de l'effet des probiotiques « Pediococcus acidilactici » et « Saccharomyces cerevisiae ssp. boulardii » sur le microbiote intestinal et sur les réponses innées et acquises lors d'une infection à « Salmonella Typhimurium DT 104 ».” 2013. Thesis, Université Laval. Accessed January 17, 2021. http://hdl.handle.net/20.500.11794/24266.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Brousseau, Jean-Philippe. “Évaluation chez le porcelet de l'effet des probiotiques « Pediococcus acidilactici » et « Saccharomyces cerevisiae ssp. boulardii » sur le microbiote intestinal et sur les réponses innées et acquises lors d'une infection à « Salmonella Typhimurium DT 104 ».” 2013. Web. 17 Jan 2021.

Vancouver:

Brousseau J. Évaluation chez le porcelet de l'effet des probiotiques « Pediococcus acidilactici » et « Saccharomyces cerevisiae ssp. boulardii » sur le microbiote intestinal et sur les réponses innées et acquises lors d'une infection à « Salmonella Typhimurium DT 104 ». [Internet] [Thesis]. Université Laval; 2013. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/20.500.11794/24266.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Brousseau J. Évaluation chez le porcelet de l'effet des probiotiques « Pediococcus acidilactici » et « Saccharomyces cerevisiae ssp. boulardii » sur le microbiote intestinal et sur les réponses innées et acquises lors d'une infection à « Salmonella Typhimurium DT 104 ». [Thesis]. Université Laval; 2013. Available from: http://hdl.handle.net/20.500.11794/24266

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Texas A&M University

19. Muras, Tiffany Marie. Escherichia coli O157:H7 and Salmonella Typhimurium Risk Assessment during the Production of Marinated Beef Inside Skirts and Tri-tip Roasts.

Degree: MS, Food Science and Technology, 2010, Texas A&M University

URL: http://hdl.handle.net/1969.1/ETD-TAMU-2009-08-7206

► This study was conducted to determine the survival of Escherichia coli O157:H7 and Salmonella Typhimurium in marinade that was used to vacuum tumble beef inside… (more)

▼ This study was conducted to determine the survival of Escherichia coli O157:H7 and Salmonella Typhimurium in marinade that was used to vacuum tumble beef inside skirts and beef tri-tip roasts. The depth of penetration of each microorganism into the individual meat products, and the survival of these microorganisms in the products as well as marinade stored over time were evaluated. Two commercial marinades were used, Reo TAMU Fajita Marinade and Legg's Cajun Style Marinade. Eighteen beef inside skirts and 18 tri-tips were used during this study. Both inside skirts and tri-tips were vacuum tumbled for a total of 1 h. Samples of products were tested immediately following tumbling (day 0), or were vacuum packaged and stored in the cooler (approximately 2 degrees C) to be tested 7 and 14 days following tumbling. Samples of the spent marinade were taken and tested initially following tumbling (day 0), and were also stored in a cooler and tested 3 and 7 days after the marinade was used. The results of the study showed that with both marinades S. Typhimurium and E. coli O157:H7 penetrated throughout the skirt meat. After having been stored for 7 days following tumbling, the log value of both S. Typhimurium and E. coli O157:H7 decreased in the meat. After 14 days of storage following tumbling, the log value of both S. Typhimurium and E. coli O157:H7 continued to decrease; however, both pathogens were still detectable. The penetration of the pathogens in the tri-tip roast varied depending on the thickness of the roast. The thicker roasts had undetectable levels of both pathogens in the geometric center; however, the thinner tri-tip roasts had detectable levels at the geometric center. The spent marinade tested on day 0, 3, and 7 showed that the microorganisms were able to survive in the marinade at refrigerated temperatures. The results of this study demonstrated that pathogens may penetrate into the interior of beef skirts and tri-tips during vacuum tumbling with contaminated marinade, and that pathogens survive during refrigerated storage of spent marinade. Industry should consider these data when evaluating potential food safety risks associated with the production of vacuum tumbling beef products. Advisors/Committee Members: Savell, Jeffrey W. (advisor), Harris, Kerri B. (advisor), Lineberger, R. Daniel (committee member), Hardin, Margaret D. (committee member).

Subjects/Keywords: E. coli O157:H7; S. Typhimurium; Inside Skirt; Tri-tip; Non-Intact Beef; Vacuum Tumble Marination

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APA (6^th Edition):

Muras, T. M. (2010). Escherichia coli O157:H7 and Salmonella Typhimurium Risk Assessment during the Production of Marinated Beef Inside Skirts and Tri-tip Roasts. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-2009-08-7206

Chicago Manual of Style (16^th Edition):

Muras, Tiffany Marie. “Escherichia coli O157:H7 and Salmonella Typhimurium Risk Assessment during the Production of Marinated Beef Inside Skirts and Tri-tip Roasts.” 2010. Masters Thesis, Texas A&M University. Accessed January 17, 2021. http://hdl.handle.net/1969.1/ETD-TAMU-2009-08-7206.

MLA Handbook (7^th Edition):

Muras, Tiffany Marie. “Escherichia coli O157:H7 and Salmonella Typhimurium Risk Assessment during the Production of Marinated Beef Inside Skirts and Tri-tip Roasts.” 2010. Web. 17 Jan 2021.

Vancouver:

Muras TM. Escherichia coli O157:H7 and Salmonella Typhimurium Risk Assessment during the Production of Marinated Beef Inside Skirts and Tri-tip Roasts. [Internet] [Masters thesis]. Texas A&M University; 2010. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2009-08-7206.

Council of Science Editors:

Muras TM. Escherichia coli O157:H7 and Salmonella Typhimurium Risk Assessment during the Production of Marinated Beef Inside Skirts and Tri-tip Roasts. [Masters Thesis]. Texas A&M University; 2010. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2009-08-7206

Université Laval

20. Sheibani, Sara. Comparison of the efficiency of two bio-pasteurization systems to eliminate escherichia coli 0157:H7 and salmonella enterica subsp. enterica serovar typhimurium in manure.

Degree: 2007, Université Laval

URL: http://hdl.handle.net/20.500.11794/19216

Subjects/Keywords: S 405 UL 2007 S543; Fumier – Décontamination; Déchets organiques en agriculture – Décontamination; Escherichia coli; Salmonella typhimurium

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APA (6^th Edition):

Sheibani, S. (2007). Comparison of the efficiency of two bio-pasteurization systems to eliminate escherichia coli 0157:H7 and salmonella enterica subsp. enterica serovar typhimurium in manure. (Thesis). Université Laval. Retrieved from http://hdl.handle.net/20.500.11794/19216

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Sheibani, Sara. “Comparison of the efficiency of two bio-pasteurization systems to eliminate escherichia coli 0157:H7 and salmonella enterica subsp. enterica serovar typhimurium in manure.” 2007. Thesis, Université Laval. Accessed January 17, 2021. http://hdl.handle.net/20.500.11794/19216.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Sheibani, Sara. “Comparison of the efficiency of two bio-pasteurization systems to eliminate escherichia coli 0157:H7 and salmonella enterica subsp. enterica serovar typhimurium in manure.” 2007. Web. 17 Jan 2021.

Vancouver:

Sheibani S. Comparison of the efficiency of two bio-pasteurization systems to eliminate escherichia coli 0157:H7 and salmonella enterica subsp. enterica serovar typhimurium in manure. [Internet] [Thesis]. Université Laval; 2007. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/20.500.11794/19216.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Sheibani S. Comparison of the efficiency of two bio-pasteurization systems to eliminate escherichia coli 0157:H7 and salmonella enterica subsp. enterica serovar typhimurium in manure. [Thesis]. Université Laval; 2007. Available from: http://hdl.handle.net/20.500.11794/19216

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Queensland

21. Qi, Xiaying. The role of sorting nexins in macropinocytosis and Salmonella invasion.

Degree: Institute for Molecular Bioscience, 2015, University of Queensland

URL: http://espace.library.uq.edu.au/view/UQ:371851

Subjects/Keywords: Macropinocytosis; Sorting nexin (SNX); S. Typhimurium; Phosphoinositides; 060108 Protein Trafficking; 060109 Proteomics and Intermolecular Interactions (excl. Medical Proteomics)

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Qi, X. (2015). The role of sorting nexins in macropinocytosis and Salmonella invasion. (Thesis). University of Queensland. Retrieved from http://espace.library.uq.edu.au/view/UQ:371851

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Qi, Xiaying. “The role of sorting nexins in macropinocytosis and Salmonella invasion.” 2015. Thesis, University of Queensland. Accessed January 17, 2021. http://espace.library.uq.edu.au/view/UQ:371851.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Qi, Xiaying. “The role of sorting nexins in macropinocytosis and Salmonella invasion.” 2015. Web. 17 Jan 2021.

Vancouver:

Qi X. The role of sorting nexins in macropinocytosis and Salmonella invasion. [Internet] [Thesis]. University of Queensland; 2015. [cited 2021 Jan 17]. Available from: http://espace.library.uq.edu.au/view/UQ:371851.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Qi X. The role of sorting nexins in macropinocytosis and Salmonella invasion. [Thesis]. University of Queensland; 2015. Available from: http://espace.library.uq.edu.au/view/UQ:371851

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

22. Pultz, Ingrid Swanson. The cyclic-di-GMP receptors of S. Typhimurium: testing their signaling specificity through second messenger affinity and their use as biosensors.

Degree: PhD, 2013, University of Washington

URL: http://hdl.handle.net/1773/22551

► c-di-GMP is a second messenger that regulates motility and the production of adhesive factors in many bacterial species. Enzymes containing specific c-di-GMP metabolizing domains integrate… (more)

▼ c-di-GMP is a second messenger that regulates motility and the production of adhesive factors in many bacterial species. Enzymes containing specific c-di-GMP metabolizing domains integrate information about the environment into an intracellular level of c-di-GMP that then binds to specific downstream receptors, including proteins that contain the PilZ domain. Many bacterial species encode dozens of c-di-GMP metabolizing enzymes in their genomes. Although each of these enzymes metabolizes the same small, diffusible second messenger molecule, many of these proteins can be specifically linked to downstream c-di-GMP-regulated processes. The mechanisms involved in achieving this signaling specificity between c-di-GMP metabolizing enzymes and their downstream receptors are not known. Here, we provide evidence that c-di-GMP signaling specificity is achieved through differences in the binding affinities of downstream receptors. Salmonella Typhimurium harbors two PilZ domain proteins: YcgR, which controls flagellar-based motility, and BcsA, an enzyme that produces cellulose. Using a Forster resonance energy transfer (FRET)-based method, we measured the binding affinities of these PilZ domain proteins and found that they span a 43-fold range. Increasing the binding affinity of BcsA for c-di-GMP increased the amount of cellulose that this enzyme produced at lower levels of c-di-GMP. Decreasing the affinity of YcgR for c-di-GMP increased the amount of this second messenger needed for YcgR to inhibit motility. In addition, we found that mutation in yhjH, which encodes a predicted c-di-GMP-degrading enzyme, increased the fraction of the cellular population that demonstrated c-di-GMP levels high enough to bind to the higher-affinity YcgR protein, but did not enough to bind to the lower-affinity BcsA protein and stimulate cellulose production. Thus, the specific affinities of these proteins for c-di-GMP are important for their biological functions. Additionally, the binding affinities of the eight PilZ domain proteins in Pseudomonas aeruginosa were measured and found to span a 145-fold range, implying that regulation by binding affinity of downstream receptors for c-di-GMP may be a common theme in c-di-GMP signaling. Finally, we generated a panel of FRET-based c-di-GMP biosensors which will allow for the accurate measurement of the free c-di-GMP level in individual cells from the nanomolar to the micromolar range. Advisors/Committee Members: Miller, Samuel I (advisor).

Subjects/Keywords: BcsA; c-di-GMP; FRET; PilZ; S. Typhimurium; YcgR; Microbiology; microbiology

…suggest two binding sites �� . ��.71 Figure 4.1: S. Typhimurium makes cellulose at 24°C, but not… …translational level �� …. ��.93 Figure 4.3: The S. Typhimurium YcgR protein contains an arginine in… …Position-X ��…. ��.94 Figure 4.4: Mutation of the “Position-X” residues in S. Typhimurium PilZ… …domain proteins…..95 Figure 4.5: A S. Typhimurium strain harboring a chromosomal arabinose… …inducible DGC ��.96 Figure 4.6: A S. Typhimurium strain harboring the bcsA-V695R mutation…

10 more images…

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Pultz, I. S. (2013). The cyclic-di-GMP receptors of S. Typhimurium: testing their signaling specificity through second messenger affinity and their use as biosensors. (Doctoral Dissertation). University of Washington. Retrieved from http://hdl.handle.net/1773/22551

Chicago Manual of Style (16^th Edition):

Pultz, Ingrid Swanson. “The cyclic-di-GMP receptors of S. Typhimurium: testing their signaling specificity through second messenger affinity and their use as biosensors.” 2013. Doctoral Dissertation, University of Washington. Accessed January 17, 2021. http://hdl.handle.net/1773/22551.

MLA Handbook (7^th Edition):

Pultz, Ingrid Swanson. “The cyclic-di-GMP receptors of S. Typhimurium: testing their signaling specificity through second messenger affinity and their use as biosensors.” 2013. Web. 17 Jan 2021.

Vancouver:

Pultz IS. The cyclic-di-GMP receptors of S. Typhimurium: testing their signaling specificity through second messenger affinity and their use as biosensors. [Internet] [Doctoral dissertation]. University of Washington; 2013. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/1773/22551.

Council of Science Editors:

Pultz IS. The cyclic-di-GMP receptors of S. Typhimurium: testing their signaling specificity through second messenger affinity and their use as biosensors. [Doctoral Dissertation]. University of Washington; 2013. Available from: http://hdl.handle.net/1773/22551

23. Vieira, Laura Cardozo. Obtenção de derivados semissintéticos triterpênicos do ácido ursólico visando atividade biológica.

Degree: 2013, Universidade do Rio Grande do Sul

URL: http://hdl.handle.net/10183/81823

►

As infecções por bactérias representam um grave problema hoje e para o futuro, devido ao fato de que estes microrganismos desenvolvem mecanismos de resistência aos… (more)

▼

As infecções por bactérias representam um grave problema hoje e para o futuro, devido ao fato de que estes microrganismos desenvolvem mecanismos de resistência aos antibióticos ao longo do tempo de uso. A formação de biofilmes também é um fator a ser discutido no cenário atual por estar associado a muitas infecções bacterianas humanas, principalmente àquelas envolvendo dispositivos médicos aumentando assim os riscos de infecções hospitalares. O ácido ursólico (AU) é um triterpeno conhecido por suas atividades biológicas relatadas. Apresenta moderada atividade antibacteriana, porém tem demonstrado importante citotoxicidade frente a algumas linhagens celulares. Em vista disso, neste trabalho se desenvolveu uma série de novas moléculas derivadas do AU com alterações nas posições C-3 e C-28. Quatro moléculas com substituição em C-3 (derivados 2, 3, 4e 5) e uma com substituição em C-3 e C-28 (derivado 6) foram comparadas ao AU (1) quanto a atividade antibacteriana. As cepas utilizadas foram Salmonela Typhimurium, Escherichia coli, Pseudomonas aeruginosa, Acinetobacter baumannii, Proteus mirabilis, Staphylococcus epidermidis e Staphylococcus aureus. Os compostos 3 e 6 apresentaram melhor perfil inibitório de forma geral, onde 3 apresentouse bactericida para S. aureus e S. epidermidis (Gram positivas) e paraP. mirabilis (Gram negativa) apresentou-se bacteriostático.

The ursolic acid (UA) is a triterpene known for their biological activities reported. Thus, become useful techniques semi-synthesis starting from natural products extracted, for example residue industries in order to improve the pharmacological properties decreasing toxicity. The bacterial infections are a serious problem today and in the future due to the fact that these organisms develop resistance mechanisms to antibiotics over time of use. The formation of biofilms is also a factor to be discussed in the current scenario because of being responsible for a very high number of rejections and other prosthetic devices by increasing the risk of nosocomial infections. The AU has a moderate antibacterial activity reported in the literature, but showed significant cytotoxicity against some cell lines. In view of this it developed a series of new molecules derived from AU residues extracted from apples (Mallus domestica) from the juice industry by promoting the so-called green chemistry. The molecules undergo changes in C-3 and C-28. Four molecules with substitution at C-3 (derived from 2, 3, 4 and 5) and one with substitution at C-3 and C-28 (derived 6) were compared with au (1). The strains used in the tests of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration were Salmonella Typhimurium, Escherichia coli, Pseudomonas aeruginosa, Acinetobacter baumannii, Proteus mirabilis, Staphylococcus epidermidis and Staphylococcus aureus. Compounds 3 and 6 had better inhibitory profile in general, where three presented bactericidal to S. aureus and S. epidermidis (Gram positive) and P. mirabilis (Gram negative) appeared bacteriostatic.

Advisors/Committee Members: Gnoatto, Simone Cristina Baggio.

Subjects/Keywords: Ácido ursólico; Ursolic acid; Triterpenos; Semi-synthesis; Atividade antibacteriana; Bacterial infections; Triterpenes; Atividade antibiofilme; Microbiologia; S. aureus; Sintese organica; S. epidermidis; P. mirabilis; S. typhimurium; E. coli; P. aeruginosa; A. baumannii

…mirabilis, S. typhimurium, E. coli, P. aeruginosa, A. baumannii. ABSTRACT The ursolic acid… …mirabilis, S. typhimurium, E. coli, P. aeruginosa, A. baumannii. LISTA DE FIGURAS FIGURA 1… …sorotipo Typhimurium (S. typhimurium) sobrevive no trato digestivo de aves, muitas… …typhimurium, S. aureus (ATCC 25923), E. coli e P. aeruginosa(ATCC 27853). Foram… …antibacteriana. As cepas utilizadas foram Salmonela Typhimurium, Escherichia coli, Pseudomonas…

11 more images…

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APA (6^th Edition):

Vieira, L. C. (2013). Obtenção de derivados semissintéticos triterpênicos do ácido ursólico visando atividade biológica. (Thesis). Universidade do Rio Grande do Sul. Retrieved from http://hdl.handle.net/10183/81823

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Vieira, Laura Cardozo. “Obtenção de derivados semissintéticos triterpênicos do ácido ursólico visando atividade biológica.” 2013. Thesis, Universidade do Rio Grande do Sul. Accessed January 17, 2021. http://hdl.handle.net/10183/81823.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Vieira, Laura Cardozo. “Obtenção de derivados semissintéticos triterpênicos do ácido ursólico visando atividade biológica.” 2013. Web. 17 Jan 2021.

Vancouver:

Vieira LC. Obtenção de derivados semissintéticos triterpênicos do ácido ursólico visando atividade biológica. [Internet] [Thesis]. Universidade do Rio Grande do Sul; 2013. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/10183/81823.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Vieira LC. Obtenção de derivados semissintéticos triterpênicos do ácido ursólico visando atividade biológica. [Thesis]. Universidade do Rio Grande do Sul; 2013. Available from: http://hdl.handle.net/10183/81823

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

24. Corcoran, Mary. Salmonella enterica - biofilm formation and survival of disinfection treatment on food contact surfaces.

Degree: 2013, National University of Ireland – Galway

URL: http://hdl.handle.net/10379/3515

► A recent report indicated that Salmonella was the most frequently identified agent in food-borne outbreaks in Europe. Salmonella can survive on surfaces and act as… (more)

▼ A recent report indicated that Salmonella was the most frequently identified agent in food-borne outbreaks in Europe. Salmonella can survive on surfaces and act as a source of cross-contamination. Biofilm formation may allow Salmonella enterica survival on surfaces and persistence in food processing environments for extensive periods of time. In 2008 there was a large food-borne outbreak of Salmonella Agona linked to a food processing facility. Prior to this outbreak, S. Agona was less frequently implicated in food-borne infections. The S. Agona strain SAGOXB.0066 and a variant strain remained in the environment for an extensive period of time. This lead to the hypothesis that the S. Agona strain may have a high propensity to form a biofilm and withstand disinfection treatment. The objectives of this research were to investigate if S. Agona SAGOXB.0066 may form a more dense biofilm than other S. enterica strains. Secondly, to investigate if S. enterica biofilm density increased over an extended period of time. Thirdly, to examine if S. enterica biofilm results in enhanced bacterial survival post disinfection treatment. The results indicate there was no evidence to suggest that S. Agona SAGOXB.0066 or the variant strain formed a more dense biofilm than other S. Agona strains after 48-hours. However there is some limited evidence to suggest that the serovar S. Agona may from more dense biofilm than other serovars. The results also indicate that S. enterica strains were able to withstand disinfectant treatment after biofilm formation. A number of S. enterica biofilm studies have tended to examine a limited number of strains or a single biofilm substratum. This research enhances the understanding of the S. enterica biofilm formation through the use of multiple strains. Moreover, the results illustrate the extent to which variation in repeated measurements on the same strain or the impact of different biofilm development models may contribute to apparent difference between strains examined. Advisors/Committee Members: Cormican, Martin (advisor), Morris, Dearbhaile (advisor).

Subjects/Keywords: Salmonella; Salmonella enterica; S. Agona; S. Typhimurium; S. Enteritidis; Biofilm formation; Food contact surfaces; Glass; Steel; Polycarbonate; Glazed tile; Concrete; CDC Biofilm Reactor; 48 hour biofilm; 168 hour biofilm; Salmonella biofilm tolerance to disinfectants

…Agona strains 72 2.4 Mean log10 density and standard deviation of S. 74 Typhimurium… …Incomplete removal of S. Typhimurium LT 2 on glass, 68 steel, polycarbonate, tile and concrete 2.6… …Complete removal of S. Typhimurium SL1344 from glass, 69 steel polycarbonate, tile and concrete… …Appendix 3- Figure 2: PFGE Image of S. Typhimurium 265 and monophasic S. Typhimurium STYMXB.131… …x29;, S. Typhimurium and S. Enteritidis. Table 1.1: Organisation of Salmonella species and…

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APA (6^th Edition):

Corcoran, M. (2013). Salmonella enterica - biofilm formation and survival of disinfection treatment on food contact surfaces. (Thesis). National University of Ireland – Galway. Retrieved from http://hdl.handle.net/10379/3515

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Corcoran, Mary. “Salmonella enterica - biofilm formation and survival of disinfection treatment on food contact surfaces. ” 2013. Thesis, National University of Ireland – Galway. Accessed January 17, 2021. http://hdl.handle.net/10379/3515.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Corcoran, Mary. “Salmonella enterica - biofilm formation and survival of disinfection treatment on food contact surfaces. ” 2013. Web. 17 Jan 2021.

Vancouver:

Corcoran M. Salmonella enterica - biofilm formation and survival of disinfection treatment on food contact surfaces. [Internet] [Thesis]. National University of Ireland – Galway; 2013. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/10379/3515.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Corcoran M. Salmonella enterica - biofilm formation and survival of disinfection treatment on food contact surfaces. [Thesis]. National University of Ireland – Galway; 2013. Available from: http://hdl.handle.net/10379/3515

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

25. Levy, Jason M. Evaluation of Peanut Skin Extract, Grape Seed Extract, and Grape Seed Extract Fractions to Reduce Populations of Select Foodborne Pathogens.

Degree: MSin Life Sciences, Food Science and Technology, 2014, Virginia Tech

URL: http://hdl.handle.net/10919/48896

► Grape seed extract (GSE) and peanut skin extract (PSE) are waste products in the wine and peanut industries. Both extracts have high concentrations of polyphenols,… (more)

Subjects/Keywords: Polyphenols; natural antimicrobials; grape seed extract; Vitis vinifera; peanut skin extract; E. coli O157:H7; Listeria monocytogenes; S. aureus; Salmonella Typhimurium

…13 S. Typhimurium… …aureus, E. coli O157:H7, and S. Typhimurium at 35°C… …64 4. Figure 4. Growth curves for S. Typhimurium in the presence of oligomer fractions… …four pathogenic bacteria (L. monocytogenes, S. aureus, S. enterica serovar Typhimurium… …S. Typhimurium. Polyphenols Polyphenols are defined as a compound containing more than one…

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Levy, J. M. (2014). Evaluation of Peanut Skin Extract, Grape Seed Extract, and Grape Seed Extract Fractions to Reduce Populations of Select Foodborne Pathogens. (Masters Thesis). Virginia Tech. Retrieved from http://hdl.handle.net/10919/48896

Chicago Manual of Style (16^th Edition):

Levy, Jason M. “Evaluation of Peanut Skin Extract, Grape Seed Extract, and Grape Seed Extract Fractions to Reduce Populations of Select Foodborne Pathogens.” 2014. Masters Thesis, Virginia Tech. Accessed January 17, 2021. http://hdl.handle.net/10919/48896.

MLA Handbook (7^th Edition):

Levy, Jason M. “Evaluation of Peanut Skin Extract, Grape Seed Extract, and Grape Seed Extract Fractions to Reduce Populations of Select Foodborne Pathogens.” 2014. Web. 17 Jan 2021.

Vancouver:

Levy JM. Evaluation of Peanut Skin Extract, Grape Seed Extract, and Grape Seed Extract Fractions to Reduce Populations of Select Foodborne Pathogens. [Internet] [Masters thesis]. Virginia Tech; 2014. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/10919/48896.

Council of Science Editors:

Levy JM. Evaluation of Peanut Skin Extract, Grape Seed Extract, and Grape Seed Extract Fractions to Reduce Populations of Select Foodborne Pathogens. [Masters Thesis]. Virginia Tech; 2014. Available from: http://hdl.handle.net/10919/48896

Open Access Theses and Dissertations