subject:(Ribosomal RNA)

University of Guelph

1. Eagle, Shannon H. C. Copy number variation of ribosomal RNA genes and the Pokey DNA transposon in the Daphnia pulex species complex.

Degree: MS, Department of Integrative Biology, 2013, University of Guelph

URL: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/6574

► There are two full length variants of the Pokey DNA transposon, PokeyA and PokeyB, and two MITEs, mPok1 and mPok2. Pokey inserts into ribosomal DNA… (more)

Subjects/Keywords: Daphnia; transposons; Pokey; ribosomal RNA genes

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APA (6^th Edition):

Eagle, S. H. C. (2013). Copy number variation of ribosomal RNA genes and the Pokey DNA transposon in the Daphnia pulex species complex. (Masters Thesis). University of Guelph. Retrieved from https://atrium.lib.uoguelph.ca/xmlui/handle/10214/6574

Chicago Manual of Style (16^th Edition):

Eagle, Shannon H C. “Copy number variation of ribosomal RNA genes and the Pokey DNA transposon in the Daphnia pulex species complex.” 2013. Masters Thesis, University of Guelph. Accessed April 17, 2021. https://atrium.lib.uoguelph.ca/xmlui/handle/10214/6574.

MLA Handbook (7^th Edition):

Eagle, Shannon H C. “Copy number variation of ribosomal RNA genes and the Pokey DNA transposon in the Daphnia pulex species complex.” 2013. Web. 17 Apr 2021.

Vancouver:

Eagle SHC. Copy number variation of ribosomal RNA genes and the Pokey DNA transposon in the Daphnia pulex species complex. [Internet] [Masters thesis]. University of Guelph; 2013. [cited 2021 Apr 17]. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/6574.

Council of Science Editors:

Eagle SHC. Copy number variation of ribosomal RNA genes and the Pokey DNA transposon in the Daphnia pulex species complex. [Masters Thesis]. University of Guelph; 2013. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/6574

University of Manitoba

2. Zachariah, Robby. Investigating MeCP2 isoform-specific expression and function.

Degree: Biochemistry and Medical Genetics, 2012, University of Manitoba

URL: http://hdl.handle.net/1993/32008

► Methyl CpG Binding Protein 2 (MeCP2) is an epigenetic regulator capable of recognizing and binding to methylated DNA. Mutations in MECP2 are the primary cause… (more)

▼ Methyl CpG Binding Protein 2 (MeCP2) is an epigenetic regulator capable of recognizing and binding to methylated DNA. Mutations in MECP2 are the primary cause of Rett Syndrome (RTT) and MECP2 Duplication Syndrome (MDS). RTT is a neurodevelopmental disorder that mainly affects young females. MDS on the other hand is 100% penetrant in males and is rarely reported in females. The two disorders, although caused by extremely different etiologies, exhibit many similarities in their phenotypes including but not limited to autistic features, learning impairments and seizures. However, the molecular basis of this phenotypic similarity remains unknown. No cure has been identified to date for RTT and MDS. Alternative splicing of Mecp2/MECP2 leads to the generation of two isoforms, MeCP2E1 and MeCP2E2. Limited knowledge exists on the expression patterns and function of the two isoforms. In this thesis, I have attempted to address this knowledge gap by taking part in the validation of custom-made MeCP2 isoform-specific antibodies that are capable of differentially recognizing MeCP2E1 and MeCP2E2. Using the custom-made MeCP2E1-specific antibody, I also demonstrate that MeCP2E1 is expressed at much higher levels in neurons, as compared to astrocytes. My studies into the functional role of MeCP2 isoforms in neurons suggest that overexpression of both MECP2E1 and MECP2E2 leads to reduced rRNA levels in neurons. The potential role of MeCP2 as a negative regulator of neuronal rRNA biogenesis is further corroborated by direct binding of MeCP2 to the rDNA promoter, specifically the methylated fraction of rDNAs. Preliminary evidence from my studies suggests that MECP2 duplication in mice leads to brain region-specific alterations in rRNA levels, specifically in the cerebellum. Thus, the data presented in this thesis addresses two important knowledge gaps in the field of MeCP2 research: the higher levels of MeCP2E1 in neurons compared to astrocytes and the molecular consequences of MECP2E1 and MECP2E2 overexpression in neurons. Advisors/Committee Members: Rastegar, Mojgan (Biochemistry and Medical Genetics) (supervisor), Davie, James (Biochemistry and Medical Genetics).

Subjects/Keywords: MeCP2; Ribosomal RNA; MeCP2-associated Disorders

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APA (6^th Edition):

Zachariah, R. (2012). Investigating MeCP2 isoform-specific expression and function. (Thesis). University of Manitoba. Retrieved from http://hdl.handle.net/1993/32008

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Zachariah, Robby. “Investigating MeCP2 isoform-specific expression and function.” 2012. Thesis, University of Manitoba. Accessed April 17, 2021. http://hdl.handle.net/1993/32008.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Zachariah, Robby. “Investigating MeCP2 isoform-specific expression and function.” 2012. Web. 17 Apr 2021.

Vancouver:

Zachariah R. Investigating MeCP2 isoform-specific expression and function. [Internet] [Thesis]. University of Manitoba; 2012. [cited 2021 Apr 17]. Available from: http://hdl.handle.net/1993/32008.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Zachariah R. Investigating MeCP2 isoform-specific expression and function. [Thesis]. University of Manitoba; 2012. Available from: http://hdl.handle.net/1993/32008

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universitat Pompeu Fabra

3. Morral Martínez, Clara, 1989-. The Nucleolus : a connection between cell fate and tumorigenesis in colorectal cancer.

Degree: Departament de Ciències Experimentals i de la Salut, 2017, Universitat Pompeu Fabra

URL: http://hdl.handle.net/10803/663807

► Colorectal cancers (CRCs) are amalgams of phenotypically distinct tumor cell populations in which only a subset of cells retain the capacity to sustain tumor growth… (more)

Subjects/Keywords: Nucleol; RNA ribosomal; Càncer colorectal; Cèl.lules mare; Nucleolus; Ribosomal RNA; Colorectal Cancer; Stem cells; 576

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APA (6^th Edition):

Morral Martínez, Clara, 1. (2017). The Nucleolus : a connection between cell fate and tumorigenesis in colorectal cancer. (Thesis). Universitat Pompeu Fabra. Retrieved from http://hdl.handle.net/10803/663807

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Morral Martínez, Clara, 1989-. “The Nucleolus : a connection between cell fate and tumorigenesis in colorectal cancer.” 2017. Thesis, Universitat Pompeu Fabra. Accessed April 17, 2021. http://hdl.handle.net/10803/663807.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Morral Martínez, Clara, 1989-. “The Nucleolus : a connection between cell fate and tumorigenesis in colorectal cancer.” 2017. Web. 17 Apr 2021.

Vancouver:

Morral Martínez, Clara 1. The Nucleolus : a connection between cell fate and tumorigenesis in colorectal cancer. [Internet] [Thesis]. Universitat Pompeu Fabra; 2017. [cited 2021 Apr 17]. Available from: http://hdl.handle.net/10803/663807.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Morral Martínez, Clara 1. The Nucleolus : a connection between cell fate and tumorigenesis in colorectal cancer. [Thesis]. Universitat Pompeu Fabra; 2017. Available from: http://hdl.handle.net/10803/663807

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

4. 陳, 輝; チン, ホイ. Analysis of the RNA polymerase 1-2 gene mutant mouse line established by gene trapping : イデンシトラップホウニヨリジュリツサレタ RNA polymerase 1-2 イデンシヘンイマウスノブンセキ; 遺伝子トラップ法により樹立されたRNA polymerase 1-2 遺伝子変異マウスの解析.

Degree: Kumamoto University / 熊本大学

URL: http://hdl.handle.net/2298/11092

Ribosomal biogenesis is closely involved in cell growth and proliferation. Ribosomal RNA gene (rDNA) transcribed by RNA polymerase I (Pol I) is an important initial step for production of ribosomes.

リボゾームRNA (rRNA)の生合成は、細胞の増殖・分裂と密接に関連しており、細胞生物学的に最も普遍的かつ重要な機能の一つである。rRNAはRNA合成酵素I (RNAPol I)によって転写される。この機能が阻害されることにより、マウスの発生分化にどのような影響が生じるかを研究するため、RNA Pol Iの構成タンパクの中で2番目に大きいサブユニットをコードするRpo1-2遺伝子内に遺伝子トラップベクターが挿入された挿入変異マウスを用い、その解析を行なった。

Subjects/Keywords: ribosomal RNA; RNA polymerase Ⅰ; nucleolus

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APA (6^th Edition):

陳, 輝; チン, �. (n.d.). Analysis of the RNA polymerase 1-2 gene mutant mouse line established by gene trapping : イデンシトラップホウニヨリジュリツサレタ RNA polymerase 1-2 イデンシヘンイマウスノブンセキ; 遺伝子トラップ法により樹立されたRNA polymerase 1-2 遺伝子変異マウスの解析. (Thesis). Kumamoto University / 熊本大学. Retrieved from http://hdl.handle.net/2298/11092

Note: this citation may be lacking information needed for this citation format:
No year of publication.
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

陳, 輝; チン, ホイ. “Analysis of the RNA polymerase 1-2 gene mutant mouse line established by gene trapping : イデンシトラップホウニヨリジュリツサレタ RNA polymerase 1-2 イデンシヘンイマウスノブンセキ; 遺伝子トラップ法により樹立されたRNA polymerase 1-2 遺伝子変異マウスの解析.” Thesis, Kumamoto University / 熊本大学. Accessed April 17, 2021. http://hdl.handle.net/2298/11092.

Note: this citation may be lacking information needed for this citation format:
No year of publication.
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

陳, 輝; チン, ホイ. “Analysis of the RNA polymerase 1-2 gene mutant mouse line established by gene trapping : イデンシトラップホウニヨリジュリツサレタ RNA polymerase 1-2 イデンシヘンイマウスノブンセキ; 遺伝子トラップ法により樹立されたRNA polymerase 1-2 遺伝子変異マウスの解析.” Web. 17 Apr 2021.

Note: this citation may be lacking information needed for this citation format:
No year of publication.

Vancouver:

陳, 輝; チン �. Analysis of the RNA polymerase 1-2 gene mutant mouse line established by gene trapping : イデンシトラップホウニヨリジュリツサレタ RNA polymerase 1-2 イデンシヘンイマウスノブンセキ; 遺伝子トラップ法により樹立されたRNA polymerase 1-2 遺伝子変異マウスの解析. [Internet] [Thesis]. Kumamoto University / 熊本大学; [cited 2021 Apr 17]. Available from: http://hdl.handle.net/2298/11092.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
No year of publication.

Council of Science Editors:

陳, 輝; チン �. Analysis of the RNA polymerase 1-2 gene mutant mouse line established by gene trapping : イデンシトラップホウニヨリジュリツサレタ RNA polymerase 1-2 イデンシヘンイマウスノブンセキ; 遺伝子トラップ法により樹立されたRNA polymerase 1-2 遺伝子変異マウスの解析. [Thesis]. Kumamoto University / 熊本大学; Available from: http://hdl.handle.net/2298/11092

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
No year of publication.

University of Toronto

5. Salvi, Jayesh Suresh. Perinuclear Chromosome Tethers and RNA-DNA Hybrid Suppressors Maintain Genome Stability and Cellular Lifespan.

Degree: PhD, 2015, University of Toronto

URL: http://hdl.handle.net/1807/69503

► Aging and related pathobiological conditions often involve genomic changes including alterations of repetitive DNA regions typically assembled into heterochromatin or silent chromatin structures. For example,… (more)

Subjects/Keywords: DNA-RNA Hybrid; Genome stability; ribosomal DNA; RNA regulation; Spatial genome organization; telomeres; 0493

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APA (6^th Edition):

Salvi, J. S. (2015). Perinuclear Chromosome Tethers and RNA-DNA Hybrid Suppressors Maintain Genome Stability and Cellular Lifespan. (Doctoral Dissertation). University of Toronto. Retrieved from http://hdl.handle.net/1807/69503

Chicago Manual of Style (16^th Edition):

Salvi, Jayesh Suresh. “Perinuclear Chromosome Tethers and RNA-DNA Hybrid Suppressors Maintain Genome Stability and Cellular Lifespan.” 2015. Doctoral Dissertation, University of Toronto. Accessed April 17, 2021. http://hdl.handle.net/1807/69503.

MLA Handbook (7^th Edition):

Salvi, Jayesh Suresh. “Perinuclear Chromosome Tethers and RNA-DNA Hybrid Suppressors Maintain Genome Stability and Cellular Lifespan.” 2015. Web. 17 Apr 2021.

Vancouver:

Salvi JS. Perinuclear Chromosome Tethers and RNA-DNA Hybrid Suppressors Maintain Genome Stability and Cellular Lifespan. [Internet] [Doctoral dissertation]. University of Toronto; 2015. [cited 2021 Apr 17]. Available from: http://hdl.handle.net/1807/69503.

Council of Science Editors:

Salvi JS. Perinuclear Chromosome Tethers and RNA-DNA Hybrid Suppressors Maintain Genome Stability and Cellular Lifespan. [Doctoral Dissertation]. University of Toronto; 2015. Available from: http://hdl.handle.net/1807/69503

University of British Columbia

6. Webb, Vera Ann B. In vivo in vitro synthesis of ribosomal RNA in bacillus subtilis.

Degree: PhD, Microbiology and Immunology, 1988, University of British Columbia

URL: http://hdl.handle.net/2429/29448

► The work presented explored the in vivo and in vitro synthesis of ribosomal RNA in the Gram positive, spore-forming bacterium Bacillus subtilis. The investigation began… (more)

▼ The work presented explored the in vivo and in vitro synthesis of ribosomal RNA in the Gram positive, spore-forming bacterium Bacillus subtilis. The investigation began with a study of rRNA synthesis in B. subtilis during steady state growth and under nutritional shift-up conditions. The percent of transcription which is ribosomal RNA was measured by hybridization of pulse labeled RNA to a specific DNA probe carrying the 3' end of the 23S RNA gene. The fractional rate of ribosomal RNA synthesis increased with cellular growth rate, and showed a rapid increase after a nutritional shift up. RNA synthesis during infection with an amber mutant of bacteriophage SP01 was also examined. Infected cells continued to synthesize rRNA at the preinfection rate, but could not respond to media enrichment by increasing the percent rRNA-synthesis. The latter study suggested the existence of a specific RNA polymerase that transcribed ribosomal RNA genes. The conclusions from the in vivo study led to an analysis of rRNA transcription in vitro. The isolation of the putative ribosomal RNA specific RNA polymerase was attempted by affinity chromatography on cellulose complexed with plasmid DNA containing the promoter region of the B. subtilis rrnB rRNA operon, and by sedimentation through a glycerol gradient. No difference in activity profile was observed when transcription activity at the rRNA tandem promoters was compared to activity at a non-ribosomal promoter. Since in vivo analysis of the control of rRNA synthesis in Escherichia coli suggested that regulation occurs at the level of transcription initiation, in vitro transcription initiation at the B. subtilis rRNA promoters was investigated using the single round transcription assay. Initial rates of transcription were different at each of the two tandem promoters of the B. subtilis rrnB operon: the upstream promoter, PI, initiated slowly, while the downstream promoter, P2, initiated faster. In addition, transcription initiation at the two promoters appeared to be linked. The formation of a heparin resistant complex at the PI promoter affected the stability of the heparin resistant complex formed at the P2 promoter. The kinetics of transcription initiation at the tandem rRNA promoters were examined using the tau plot analysis. RNA polymerase had a high affinity for both rRNA promoters, but the rate of initiation at these promoters was relatively slow when compared to non-ribosomal promoters. Finally, transcription initiation on two artificial tandem promoter constructs was compared with initiation on the native tandem promoter construct. In general, PI was shown to have a positive effect on transcription from downstream promoters, but had specific effects on different promoters.

Subjects/Keywords: RNA, Ribosomal; RNA – Synthesis; Bacillus subtilis

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APA (6^th Edition):

Webb, V. A. B. (1988). In vivo in vitro synthesis of ribosomal RNA in bacillus subtilis. (Doctoral Dissertation). University of British Columbia. Retrieved from http://hdl.handle.net/2429/29448

Chicago Manual of Style (16^th Edition):

Webb, Vera Ann B. “In vivo in vitro synthesis of ribosomal RNA in bacillus subtilis.” 1988. Doctoral Dissertation, University of British Columbia. Accessed April 17, 2021. http://hdl.handle.net/2429/29448.

MLA Handbook (7^th Edition):

Webb, Vera Ann B. “In vivo in vitro synthesis of ribosomal RNA in bacillus subtilis.” 1988. Web. 17 Apr 2021.

Vancouver:

Webb VAB. In vivo in vitro synthesis of ribosomal RNA in bacillus subtilis. [Internet] [Doctoral dissertation]. University of British Columbia; 1988. [cited 2021 Apr 17]. Available from: http://hdl.handle.net/2429/29448.

Council of Science Editors:

Webb VAB. In vivo in vitro synthesis of ribosomal RNA in bacillus subtilis. [Doctoral Dissertation]. University of British Columbia; 1988. Available from: http://hdl.handle.net/2429/29448

University of Cincinnati

7. Rohlfs, Rebecca L. Mass Spectrometry Analysis of Methylated Ribosomal RNA.

Degree: PhD, Arts and Sciences: Chemistry, 2013, University of Cincinnati

URL: http://rave.ohiolink.edu/etdc/view?acc_num=ucin1368024445

► The goal of this dissertation is to identify and characterize post-transcriptional modifications occurring during ribosome biogenesis. The field of bio-analytical chemistry is advanced by this… (more)

▼ The goal of this dissertation is to identify and characterize post-transcriptional modifications occurring during ribosome biogenesis. The field of bio-analytical chemistry is advanced by this research by increasing our ability to characterize and understand the process of ribosomal ribonucleic acid (rRNA) modification. The role of many post-transcriptional modifications is still largely unknown and speculated; therefore, it is essential to study the chronology of post-transcriptional modifications during ribosome biogenesis. The focus of these studies is to use liquid chromatography coupled with electrospray ionization mass spectrometry (LC-ESI-MSn) in the detection of rRNA modifications. The first study was to characterize post-transcriptional modifications within erythromycin-induced stalled ribosomal assembly intermediates. Liquid chromatography - mass spectrometry (LC-MS) was used to identify the post-transcriptional rRNA modifications present in improperly folded ribosome assembly subunits and compare these modifications to those found in properly assembled subunits. Limitations to this study led to the development of a site-specific assay for the characterization of rRNA modifications.A selected reaction-monitoring (SRM) assay was designed to detect and quantify site-specific post-transcriptional rRNA modifications. The advantage of this method is the ability to site-specifically characterize a modification that may occur multiple times within an rRNA transcript. Since some modifications occur at different times during ribosome biogenesis, it is important to quantify each modification at different stages of ribosome assembly. The SRM assay was applied to characterize post-transcriptional rRNA modifications treated with erythromycin and compare those modifications to post-transcriptional rRNA modifications of untreated cells. The work presented in this dissertation can be applied to other organisms for the construction of a timeline for rRNA modification during ribosome biogenesis. This work has progressed the field of rRNA modification and ribosome analysis by developing a new tool for the site-specific identification and characterization of rRNA modifications. Advisors/Committee Members: Limbach, Patrick (Committee Chair).

Subjects/Keywords: Analytical Chemistry; ribosomal RNA; liquid chromatography; mass spectrometry; SRM; MRM; RNA modification

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APA (6^th Edition):

Rohlfs, R. L. (2013). Mass Spectrometry Analysis of Methylated Ribosomal RNA. (Doctoral Dissertation). University of Cincinnati. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=ucin1368024445

Chicago Manual of Style (16^th Edition):

Rohlfs, Rebecca L. “Mass Spectrometry Analysis of Methylated Ribosomal RNA.” 2013. Doctoral Dissertation, University of Cincinnati. Accessed April 17, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1368024445.

MLA Handbook (7^th Edition):

Rohlfs, Rebecca L. “Mass Spectrometry Analysis of Methylated Ribosomal RNA.” 2013. Web. 17 Apr 2021.

Vancouver:

Rohlfs RL. Mass Spectrometry Analysis of Methylated Ribosomal RNA. [Internet] [Doctoral dissertation]. University of Cincinnati; 2013. [cited 2021 Apr 17]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=ucin1368024445.

Council of Science Editors:

Rohlfs RL. Mass Spectrometry Analysis of Methylated Ribosomal RNA. [Doctoral Dissertation]. University of Cincinnati; 2013. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=ucin1368024445

8. Camila Dantas Malossi. Efeitos da infecção por Rickettsia rickettsii sobre o perfil de expressão gênica do carrapato vetor Amblyomma aureolatum.

Degree: 2013, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/42/42135/tde-04062014-101149/

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Rickettsia rickettsii é o agente etiológico da Febre Maculosa das Montanhas Rochosas, que no Brasil é transmitida pelos carrapatos Amblyomma cajennense e A. aureolatum. Para… (more)

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Rickettsia rickettsii é o agente etiológico da Febre Maculosa das Montanhas Rochosas, que no Brasil é transmitida pelos carrapatos Amblyomma cajennense e A. aureolatum. Para elucidar os mecanismos de virulência sobre seus vetores, construímos bibliotecas subtrativas utilizando RNA de A. aureolatum infectados ou não com o patógeno. Com a análise bioinformática, foram obtidas 56 sequências únicas com expressão induzida e 12 com expressão reprimida pela infecção. Após a validação dos dados por RT-qPCR 3 genes foram caracterizados por RNAi: uma hebraeína, uma proteína dissulfeto isomerase (PDI) e uma proteína com domínio Kunitz-type. Um maior número de carrapatos adquiriu R. rickettsii quando a expressão gênica da hebraeína e da PDI foi silenciada, sugerindo que elas participam na defesa do carrapato contra a infecção. Nenhum efeito foi observado sobre a transmissão da bactéria para o hospedeiro ou sobre o fitness de carrapatos nos três genes analisados. O presente estudo apontou genes importantes que possibilitam uma melhor compreensão da relação carrapato-riquétsia.

Rickettsia rickettsii is the etiological agent of Rocky Mountain Spotted Fever and, in Brazil, it is transmitted by Amblyomma cajennense and A. aureolatum. To elucidate mechanisms of virulence to its vectors, we construct cDNA libraries with RNA of ticks A. aureolatum infected or not with this pathogen. After bioinformatic analysis, 56 unique sequences were obtained representing up-regulated genes and 12 down-regulated by infection. After data validation by RT- qPCR, 3 genes were characterizated by RNAi: a hebraein, a protein disulfide isomerase (PDI), and a protein with Kunitz-type domain. A higher number of ticks acquired R. rickettsii when the gene expression of hebraein and PDI was silenced, suggesting that both proteins participate in the defense of the tick against infection. No effect on the transmission of the bacterium to the host or on the fitness of ticks was observed after knockdown of the 3 analyzed genes. Data obtained by the present study pointed out important genes that provide information to better understand of the tick-rickettsia relationship.

Advisors/Committee Members: Andréa Cristina Fogaça, Pedro Lagerblad de Oliveira, Ariel Mariano Silber.

Subjects/Keywords: Amblyomma; Rickettsia; Carrapatos; Expressão gênica; RNA ribossômico; Amblyomma; Rickettsia; Gene expression; Ribosomal RNA; Ticks

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APA (6^th Edition):

Malossi, C. D. (2013). Efeitos da infecção por Rickettsia rickettsii sobre o perfil de expressão gênica do carrapato vetor Amblyomma aureolatum. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/42/42135/tde-04062014-101149/

Chicago Manual of Style (16^th Edition):

Malossi, Camila Dantas. “Efeitos da infecção por Rickettsia rickettsii sobre o perfil de expressão gênica do carrapato vetor Amblyomma aureolatum.” 2013. Masters Thesis, University of São Paulo. Accessed April 17, 2021. http://www.teses.usp.br/teses/disponiveis/42/42135/tde-04062014-101149/.

MLA Handbook (7^th Edition):

Malossi, Camila Dantas. “Efeitos da infecção por Rickettsia rickettsii sobre o perfil de expressão gênica do carrapato vetor Amblyomma aureolatum.” 2013. Web. 17 Apr 2021.

Vancouver:

Malossi CD. Efeitos da infecção por Rickettsia rickettsii sobre o perfil de expressão gênica do carrapato vetor Amblyomma aureolatum. [Internet] [Masters thesis]. University of São Paulo; 2013. [cited 2021 Apr 17]. Available from: http://www.teses.usp.br/teses/disponiveis/42/42135/tde-04062014-101149/.

Council of Science Editors:

Malossi CD. Efeitos da infecção por Rickettsia rickettsii sobre o perfil de expressão gênica do carrapato vetor Amblyomma aureolatum. [Masters Thesis]. University of São Paulo; 2013. Available from: http://www.teses.usp.br/teses/disponiveis/42/42135/tde-04062014-101149/

9. Kuhl, Leonardo Palma. Avaliação do microbioma bacteriano em cânulas de traqueostomia pediátricas em um hospital universitário brasileiro.

Degree: 2020, Brazil

URL: http://hdl.handle.net/10183/217606

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Objetivos: Avaliar o microbioma bacteriano encontrado em cânulas de traqueostomia pediátricas de um grupo de crianças com o diagnóstico de glossoptose por Sequência de Robin… (more)

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Objetivos: Avaliar o microbioma bacteriano encontrado em cânulas de traqueostomia pediátricas de um grupo de crianças com o diagnóstico de glossoptose por Sequência de Robin (SR), acompanhadas pelo Serviço de Otorrinolaringologia do Hospital de Clínicas de Porto Alegre (HCPA). Métodos: Os pacientes foram incluídos no estudo no momento da troca de cânula de traqueostomia, realizada em ambiente hospitalar e pela equipe de otorrinolaringologia. Durante esse procedimento, o aspirado traqueal foi coletado e enviado para cultura, enquanto a própria cânula de traqueostomia foi armazenada para posterior sequenciamento de amplicon do gene 16s rRNA. A extração do DNA foi realizada com o uso do kit DNeasy PowerBiofilm (QIAGEN®- Cat No. 24000-50), já o sequenciamento foi realizado seguindo o protocolo Brazilian Microbiome Project (BMP) e com o auxílio do equipamento S5 (Ion S5™ System, Thermo Fisher Scientific). Principal component analysis (PCA) foi utilizado para procurar padrões ou grupamentos entres os pacientes. Resultados: Todos os 12 pacientes estudados estavam em uso de cânulas de traqueostomia da mesma marca, sem balonete, traqueostomizados há mais de um ano e com tempo de uso da cânula de traqueostomia analisada de aproximadamente 3 meses. Entre as culturas realizadas a partir do aspirado traqueal, apenas cinco pacientes tiveram crescimento de, pelo menos, uma bactéria, porém todos estes tiveram a OTU (operational taxonomic unit) de mesmo gênero identificada em seu microbioma pela metagenômica. Foi identificado um total de 68 OTUs diferentes no nível taxonômico de gênero, sendo os encontrados com maior abundância: Aggregatibacter, Pseudomonas, Haemophilus, Neisseria, Staphylococcus, Fusobacterium, Moraxella, Streptococcus, Alloiococcus e Capnocytophaga. O microbioma individual de cada paciente apresentou grande variedade, não correlacionando com nenhuma característica clínica individual. Testes de PCA foram utilizados para comparar variáveis entre os pacientes, porém não foram encontrados grupamentos. Conclusão: O microbioma das cânulas de traqueostomias apresenta grande variedade, mesmo em pacientes com características clínicas semelhantes, sendo difícil atribuir um padrão estático de normalidade. Mais estudos são necessários para compreender as relações entre as redes de comunidades bacterianas e como elas interagem entre si, seu microambiente e as repercussões clínicas que podem causar.

Objectives: The purpose of this study was to evaluate the bacterial microbiome found in tracheostomy cannulas of a group of children diagnosed with glossoptosis secondary to Robins Sequence (RS) accompanied by the otolaryngology team of a tertiary hospital on the south of Brazil. Methods: Pediatric patients were enrolled in the study at the time of the cannula change, performed at the hospital by the otolaryngology team. During this procedure, tracheal aspirate was collected and sent to culture, while the removed cannula was collected and stored for amplicon sequencing of 16s rRNA. DNA extraction were performed using DNeasy…

Advisors/Committee Members: Schweiger, Claudia.

Subjects/Keywords: Criança; Traqueostomia; Microbiota; Metagenômica; RNA ribossômico 16S; Child; Tracheostomy; Metagenomics; Microbiota; RNA; Ribosomal; 16S

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kuhl, L. P. (2020). Avaliação do microbioma bacteriano em cânulas de traqueostomia pediátricas em um hospital universitário brasileiro. (Masters Thesis). Brazil. Retrieved from http://hdl.handle.net/10183/217606

Chicago Manual of Style (16^th Edition):

Kuhl, Leonardo Palma. “Avaliação do microbioma bacteriano em cânulas de traqueostomia pediátricas em um hospital universitário brasileiro.” 2020. Masters Thesis, Brazil. Accessed April 17, 2021. http://hdl.handle.net/10183/217606.

MLA Handbook (7^th Edition):

Kuhl, Leonardo Palma. “Avaliação do microbioma bacteriano em cânulas de traqueostomia pediátricas em um hospital universitário brasileiro.” 2020. Web. 17 Apr 2021.

Vancouver:

Kuhl LP. Avaliação do microbioma bacteriano em cânulas de traqueostomia pediátricas em um hospital universitário brasileiro. [Internet] [Masters thesis]. Brazil; 2020. [cited 2021 Apr 17]. Available from: http://hdl.handle.net/10183/217606.

Council of Science Editors:

Kuhl LP. Avaliação do microbioma bacteriano em cânulas de traqueostomia pediátricas em um hospital universitário brasileiro. [Masters Thesis]. Brazil; 2020. Available from: http://hdl.handle.net/10183/217606

University of California – Berkeley

10. Soergel, David Alexander Wolfgang. Computational Methods for Evaluating Microbial Diversity.

Degree: Biophysics, 2010, University of California – Berkeley

URL: http://www.escholarship.org/uc/item/79c2m1vm

► The design and evaluation of methods for describing the diversity of microbial life in environmental samples is a critical step towards understanding life on earth… (more)

▼ The design and evaluation of methods for describing the diversity of microbial life in environmental samples is a critical step towards understanding life on earth and towards making prudent interventions in a wide variety of microbe-driven systems.Microbes in the environment, including bacteria, archaea, viruses, and single-celled eukaryotes, are primary drivers of numerous geological and atmospheric processes, such as carbon fixation and sequestration, nutrient cycling, soil formation, and even cloud formation. Cyanobacteria in the surface of the ocean are estimated to be responsible for half of the primary production on earth. Microbes living in and on the human body are intimately involved in health and disease, even when they are not explicitly pathogenic; for instance, the gut is teeming with bacteria that are essential for digestion, but anomalies in this microbial community may contribute to disorders such as Crohn's disease. Environmental bacteria are critically important to climate change, agriculture, and public health, so understanding them has immediate practical importance, in addition to satisfying our scientific curiosity.Environmental microbiology has long been limited by the fact that over 99% of bacteria found in the environment cannot yet be cultured, because the conditions required for growth have not yet been determined. In many cases, bacteria live in interdependent communities of species, making the growth conditions extremely complex and difficult to recreate, even if they could be determined. Thus, it is not possible to perform experiments on these organisms in the lab, or to acquire sufficient DNA to sequence their genomes in isolation. These limitations can be sidestepped through the use of culture-independent surveying techniques. With the availability of ever-cheaper DNA sequencing, methods that involve direct sequencing of DNA from environmental samples have now gained prominence, and are producing a deluge of data. However, the computational methods needed to make sense of these data are still in their infancy.I evaluated methodological choices required for two kinds of culture-independent environmental sequencing techniques: taxonomic surveys using the 16S rRNA, and surveys of both taxonomy and function through shotgun sequencing. In both cases my goals were to increase the effectiveness of future studies in extracting biologically relevant information from environmental sequence datasets, and especially to head off misinterpretations of such datasets due to errors in methodology that have been overlooked to date.Microbial community composition using the 16S ribosomal RNA sequencePCR amplification and sequencing of the gene for the 16S ribosomal RNA subunit directly from environmental samples is a long-standing method of measuring species richness and relative abundance. I demonstrated that the use of sequencing reads that are much shorter than the gene itself (as has recently become economical and thus popular) has the potential to introduce substantial error in such…

Subjects/Keywords: Bioinformatics; Microbiology; Bacteria; Diversity; Metagenomics; Ribosomal RNA; Taxonomy

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Soergel, D. A. W. (2010). Computational Methods for Evaluating Microbial Diversity. (Thesis). University of California – Berkeley. Retrieved from http://www.escholarship.org/uc/item/79c2m1vm

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Soergel, David Alexander Wolfgang. “Computational Methods for Evaluating Microbial Diversity.” 2010. Thesis, University of California – Berkeley. Accessed April 17, 2021. http://www.escholarship.org/uc/item/79c2m1vm.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Soergel, David Alexander Wolfgang. “Computational Methods for Evaluating Microbial Diversity.” 2010. Web. 17 Apr 2021.

Vancouver:

Soergel DAW. Computational Methods for Evaluating Microbial Diversity. [Internet] [Thesis]. University of California – Berkeley; 2010. [cited 2021 Apr 17]. Available from: http://www.escholarship.org/uc/item/79c2m1vm.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Soergel DAW. Computational Methods for Evaluating Microbial Diversity. [Thesis]. University of California – Berkeley; 2010. Available from: http://www.escholarship.org/uc/item/79c2m1vm

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Rochester

11. Calidas, Deepika S. (1981 - ). Role of long range interactions in assembly of the small subunit of Escherichia coli ribosome.

Degree: PhD, 2012, University of Rochester

URL: http://hdl.handle.net/1802/24874

► The function of the small subunit (SSU) of the ribosome of Escherichia coli is dependent on dynamic interactions at the intersection of its four domains;… (more)

▼ The function of the small subunit (SSU) of the ribosome of Escherichia coli is dependent on dynamic interactions at the intersection of its four domains; namely, the body, platform, head and penultimate stem. The in vitro assembly of each individual domain from its corresponding structural element in 16S ribosomal RNA (rRNA), i.e., the 5’, central, 3’ major and minor domains and associated ribosomal proteins (r-proteins) has been extensively researched. Less is understood of the long range interactions that occur during assembly as different domains co-assemble, both in vitro and in vivo. Our first approach was to use directed probing from the S8 r-protein as a monitor of SSU assembly. We found that assembly of the neck, a functionally significant region between the head and platform is dependent on assembly of the body. Furthermore, S8 binds two distinct binding sites in 16S rRNA separated by several hundred nucleotides, and the appropriate architecture of the later transcribed region is dependent upon incorporation of r-proteins to the earlier transcribed region. Elements of the body domain, including the 5’ terminus do not assume their appropriate conformation except upon assembly of the entire domain. Also, we found that S12 could influence the architecture of the 5’ terminus, leading us to examine the role of S12 in 30S subunit assembly, both in vitro and in vivo. S12 possesses a non-canonically structured extension that extends from the solvent surface to the intersubunit surface of the SSU, contacting multiple domains. An almost complete truncation of the extension was unable to support growth, while partial truncations of more than 6 amino acids exhibited growth defects. Truncation of half or all of the extension also resulted in reduced activity of SSUs assembled in vitro. The architecture of ribonucleoprotein complexes assembled with truncated proteins is also altered. The work presented in this thesis elucidates influence of widely separated elements of the SSU on each other during assembly.

Subjects/Keywords: Ribonucleoprotein complexes; Ribosomal assembly; Ribosome; RNA biology; S12; S8

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APA (6^th Edition):

Calidas, D. S. (. -. ). (2012). Role of long range interactions in assembly of the small subunit of Escherichia coli ribosome. (Doctoral Dissertation). University of Rochester. Retrieved from http://hdl.handle.net/1802/24874

Chicago Manual of Style (16^th Edition):

Calidas, Deepika S (1981 - ). “Role of long range interactions in assembly of the small subunit of Escherichia coli ribosome.” 2012. Doctoral Dissertation, University of Rochester. Accessed April 17, 2021. http://hdl.handle.net/1802/24874.

MLA Handbook (7^th Edition):

Calidas, Deepika S (1981 - ). “Role of long range interactions in assembly of the small subunit of Escherichia coli ribosome.” 2012. Web. 17 Apr 2021.

Vancouver:

Calidas DS(-). Role of long range interactions in assembly of the small subunit of Escherichia coli ribosome. [Internet] [Doctoral dissertation]. University of Rochester; 2012. [cited 2021 Apr 17]. Available from: http://hdl.handle.net/1802/24874.

Council of Science Editors:

Calidas DS(-). Role of long range interactions in assembly of the small subunit of Escherichia coli ribosome. [Doctoral Dissertation]. University of Rochester; 2012. Available from: http://hdl.handle.net/1802/24874

Wayne State University

12. Seo, Hyosuk. Ligand Binding Studies Of A Peptide Targeting Helix 69 Of 23s Rrna In Bacterial Ribosomes.

Degree: PhD, Chemistry, 2017, Wayne State University

URL: https://digitalcommons.wayne.edu/oa_dissertations/1874

► In the development of finding a peptide targeting H69 of 23S rRNA in bacterial ribosomes, phage display was employed at pH 5.5, a buffer… (more)

▼ In the development of finding a peptide targeting H69 of 23S rRNA in bacterial ribosomes, phage display was employed at pH 5.5, a buffer condition previously reported of H69 preferring a closed conformation. After sequencing, several peptides were chosen through sequence alignment, followed by preparation using solid-phase peptide synthesis. The peptides were characterized using MALDI-TOF and purified with HPLC. A truncated peptide TARHIY was selected from FID assay. Through binding studies using ESI-MS, SPR, BLItz, and NMR, the binding properties of the peptide to H69 were determined, such as binding affinity, stoichiometry, and interaction site. The peptide exhibited moderate binding affinity towards H69 (apparent Kd~10 µM) using ESI-MS, SPR, and BLItz, and the methods correspond to each other, however, no selectivity towards a buffer condition or RNA type was detected. Data obtained from ESI-MS suggested dimeric binding at higher concentrations, which was explored in the later part of the research. The interaction site of the peptide towards H69 was explored using NMR, which was in the loop region of H69. Multimeric binding of peptides to H69 was explored by using dimeric peptides. Dimeric peptides with the same or different sequences on amino groups of lysine were prepared using solid-phase peptide synthesis. Improved binding affinity (apparent Kd~1 µM) of the dimer towards H69 compared to the monomer peptide were obtained using ESI-MS and BLItz. The binding affinity of the dimer TT was comparable to neomycin, a known aminoglycoside, while reverse dimer TY, exhibited decrease in binding affinity, suggesting the N-terminus plays an important role in binding, and that the 1:2 RNA:peptide complexes observed in ESI-MS spectra were not solely due to aggregation. An overall conformational change was observed with dimer TT using NMR, which was also comparable to neomycin. Further studies will help elucidate the actual binding mode of peptide TARHIY towards H69. These results suggest the possibility of multimeric binding should be taken into consideration with peptides selected from phage display. Advisors/Committee Members: Christine S. Chow.

Subjects/Keywords: binding; helix 69; peptides; phage display; ribosomal RNA; Biochemistry; Chemistry

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Seo, H. (2017). Ligand Binding Studies Of A Peptide Targeting Helix 69 Of 23s Rrna In Bacterial Ribosomes. (Doctoral Dissertation). Wayne State University. Retrieved from https://digitalcommons.wayne.edu/oa_dissertations/1874

Chicago Manual of Style (16^th Edition):

Seo, Hyosuk. “Ligand Binding Studies Of A Peptide Targeting Helix 69 Of 23s Rrna In Bacterial Ribosomes.” 2017. Doctoral Dissertation, Wayne State University. Accessed April 17, 2021. https://digitalcommons.wayne.edu/oa_dissertations/1874.

MLA Handbook (7^th Edition):

Seo, Hyosuk. “Ligand Binding Studies Of A Peptide Targeting Helix 69 Of 23s Rrna In Bacterial Ribosomes.” 2017. Web. 17 Apr 2021.

Vancouver:

Seo H. Ligand Binding Studies Of A Peptide Targeting Helix 69 Of 23s Rrna In Bacterial Ribosomes. [Internet] [Doctoral dissertation]. Wayne State University; 2017. [cited 2021 Apr 17]. Available from: https://digitalcommons.wayne.edu/oa_dissertations/1874.

Council of Science Editors:

Seo H. Ligand Binding Studies Of A Peptide Targeting Helix 69 Of 23s Rrna In Bacterial Ribosomes. [Doctoral Dissertation]. Wayne State University; 2017. Available from: https://digitalcommons.wayne.edu/oa_dissertations/1874

University of Vienna

13. Heindl, Katrin. Roles of the novel 5'-Polynucleotide kinase NoI9 in ribosome synthesis.

Degree: 2010, University of Vienna

URL: http://othes.univie.ac.at/12007/

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In einer sich teilenden Zelle nehmen ribosomale RNAs 80% der gesamten zellulären RNA ein. Sie sind die Hauptkomponente der Ribosomen, grossen Komplexen aus RNA und… (more)

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In einer sich teilenden Zelle nehmen ribosomale RNAs 80% der gesamten zellulären RNA ein. Sie sind die Hauptkomponente der Ribosomen, grossen Komplexen aus RNA und Proteinen, die als “Protein-Fabriken” der Zelle eine zentrale Aufgabe ausüben. Die korrekte und effiziente Biosynthese der ribosomalen RNA ist daher grundlegend für jede Zelle. Im Nukleolus werden drei der vier ribosomalen RNAs (rRNAs), 18S, 5.8S und 28S, gemeinsam von der RNA Polymerase I zu einem einzelnen polyzistronen Precursor transkribiert. In einer geordneten Aufeinanderfolge von endo- und exonukleo-lytischen Aktivitäten maturieren diese rRNAs und werden letztendlich gemeinsam mit der 5S rRNA und einer Vielzahl von ribosomalen Proteinen zum Ribosom zusammengefügt. Während meiner Doktorarbeit habe ich die erste nukleoläre Polynukleotid-Kinase Nol9/Grc3 identifiziert und ihre Funktion studiert. Nol9/Grc3 ist in zweierlei Hinsicht an der Maturierung von ribosomalen RNAs beteiligt: 1. S. cerevisiae Grc3 ist essenziell für eine effiziente Beendigung der Transkription von rRNA Genen als Teil des Rat1-abhängigen ‘Torpedo-Mechanismus” und somit wesentlich für die rasche Wiederverwendung der RNA Polymerase I. 2. Humanes Nol9 hat eine Funktion bei der Prozessierung der 5.8S und 28S ribosomalen RNA aus ihrem gemeinsamen Precursor, möglicherweise in einem Xrn2-abhängigen Prozessierungsschritt. Diese Ergebnisse beleuchten ein moegliches Zusammenspiel der Polynukleotid-Kinase Nol9/Grc3 und der 5’-3’ Exonuklease Xrn2/Rat1. Berücksichtigt man die wachsenden Berichte über zusätzliche Aufgaben des Nukleolus im RNA Metabolismus abseits der Maturierung ribosomaler RNA und die Aktivitaet von Nol9/Grc3 an sowohl einzel- als auch doppelsträngigen RNA- und DNA-Substraten, zeichnen sich mögliche weitere Funktionen für die Polynukleotid-Kinase Nol9/Grc3 ab.

In a dividing cell, about 80% of the cellular RNA consist of ribosomal RNAs (rRNAs), the core components of the ribosomes. Efficient protein production relies on the sufficient availability of ribosomes; therefore indefectible rRNA processing is fundamental to every cell. The 18S, 5.8S and 28S rRNAs are transcribed in the nucleolus by RNA polymerase I as a single polycistronic precursor RNA and liberated by a complex series of endo- and exonucleolytic cleavage events. Eventually, they are assembled together with the 5S rRNA and a plethora of ribosomal proteins to form the ribosomes. In my PhD work, I identified the first nucleolar polynucleotide kinase Nol9/Grc3 and investigated its involvement in two different aspects of rRNA biogenesis: 1. RNA Polymerase I transcription termination; and 2. processing of the large subunit rRNAs. In yeast, Grc3 is essential for efficient transcription termination in the Rat1-dependent ‘torpedo mechanism’ thereby enabling rapid recycling of RNA polymerase I. In human cells, Nol9 is required for the processing of 5.8S and 28S rRNAs, the components of the large 60S ribosomal subunits, most likely at a Xrn2-dependent processing step. These findings intrigue a potential…

Subjects/Keywords: 42.13 Molekularbiologie; Kinase / rRNA / Ribosom; Polynucleotide kinase / ribosomal RNA / ribosome synthesis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Heindl, K. (2010). Roles of the novel 5'-Polynucleotide kinase NoI9 in ribosome synthesis. (Thesis). University of Vienna. Retrieved from http://othes.univie.ac.at/12007/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Heindl, Katrin. “Roles of the novel 5'-Polynucleotide kinase NoI9 in ribosome synthesis.” 2010. Thesis, University of Vienna. Accessed April 17, 2021. http://othes.univie.ac.at/12007/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Heindl, Katrin. “Roles of the novel 5'-Polynucleotide kinase NoI9 in ribosome synthesis.” 2010. Web. 17 Apr 2021.

Vancouver:

Heindl K. Roles of the novel 5'-Polynucleotide kinase NoI9 in ribosome synthesis. [Internet] [Thesis]. University of Vienna; 2010. [cited 2021 Apr 17]. Available from: http://othes.univie.ac.at/12007/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Heindl K. Roles of the novel 5'-Polynucleotide kinase NoI9 in ribosome synthesis. [Thesis]. University of Vienna; 2010. Available from: http://othes.univie.ac.at/12007/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Boston College

14. Fu, Yang. Identification and Characterization of Novel Ribosomal Protein-binding RNA motifs in Bacteria.

Degree: MS, Biology, 2014, Boston College

URL: http://dlib.bc.edu/islandora/object/bc-ir:101163

► As the factory responsible for producing proteins, ribosomes are of great importance. In bacteria, ribosomes are composed of three ribosomal RNAs (rRNA) of different sizes,… (more)

Subjects/Keywords: Bacteria; cis-regulatory; L1; Ribosomal protein; RNA; S6

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Fu, Y. (2014). Identification and Characterization of Novel Ribosomal Protein-binding RNA motifs in Bacteria. (Masters Thesis). Boston College. Retrieved from http://dlib.bc.edu/islandora/object/bc-ir:101163

Chicago Manual of Style (16^th Edition):

Fu, Yang. “Identification and Characterization of Novel Ribosomal Protein-binding RNA motifs in Bacteria.” 2014. Masters Thesis, Boston College. Accessed April 17, 2021. http://dlib.bc.edu/islandora/object/bc-ir:101163.

MLA Handbook (7^th Edition):

Fu, Yang. “Identification and Characterization of Novel Ribosomal Protein-binding RNA motifs in Bacteria.” 2014. Web. 17 Apr 2021.

Vancouver:

Fu Y. Identification and Characterization of Novel Ribosomal Protein-binding RNA motifs in Bacteria. [Internet] [Masters thesis]. Boston College; 2014. [cited 2021 Apr 17]. Available from: http://dlib.bc.edu/islandora/object/bc-ir:101163.

Council of Science Editors:

Fu Y. Identification and Characterization of Novel Ribosomal Protein-binding RNA motifs in Bacteria. [Masters Thesis]. Boston College; 2014. Available from: http://dlib.bc.edu/islandora/object/bc-ir:101163

University of Notre Dame

15. Michelle Marie Bertke. The Role of Sumoylation in Early Development of Xenopus laevis and Regulation of 5S Ribosomal RNA Genes</h1>.

Degree: Chemistry and Biochemistry, 2014, University of Notre Dame

URL: https://curate.nd.edu/show/zw12z31988j

► The 5S rRNA gene-specific transcription factor, TFIIIA, interacts with the SUMO E3 ligase, PIAS2b, and with one of its targets, the transcriptional corepressor XCtBP.… (more)

▼ The 5S rRNA gene-specific transcription factor, TFIIIA, interacts with the SUMO E3 ligase, PIAS2b, and with one of its targets, the transcriptional corepressor XCtBP. PIAS2b and XCtBP are present on the oocyte, but not somatic, 5S rRNA genes up through the gastrula-neurula transition, as is a limiting amount of TFIIIA. Histone H3 methylation, coincident with the binding of XCtBP, also occurs exclusively on the oocyte genes. Immunohistochemical staining of embryos confirms occupancy of some fraction of the oocyte genes by TFIIIA that become positioned at the nuclear periphery shortly after the midblastula transition. SUMOylation can be inhibited through injection of mRNA encoding the adenovirus protein Gam1, which decreases the levels of the E1 activating enzyme by triggering its proteolytic degradation. Gam1-induced decrease in SUMOylation activity relieves repression of the oocyte 5S rRNA genes and is correlated with a decrease in methylation of H3K9 and H3K27. These results reveal a novel function for TFIIIA as a negative regulator that recruits histone modification activity, through the CtBP repressor complex, exclusively to the oocyte 5S rRNA genes, resulting in their terminal repression. SUMOylation deficient embryos also exhibit a range of important developmental defects including failure of the blastopore and neural tube to close, shortened axis, fused eyes, and perturbed heart development. Embryos injected with Gam1 mRNA or water (control) were taken for microarray analysis at three developmental time points: early gastrula, late gastrula, and early neurula. A bioinformatics analysis of this data was conducted using the MetaCore® suite of programs, BiNGO, DAVID, and the Gene Ontology database. Functional enrichment analysis of the differentially expressed genes demonstrates that SUMOylation regulates the expression of genes that span several different biological processes during early embryogenesis. Bioinformatics analysis provides evidence that, in some cases, SUMOylation generates two pools of a given transcription factor that control different subsets of genes. Although SUMOylation impacts a large variety of processes, certain signaling pathways appear to be particularly sensitive to the loss of this modification and can account for the observed phenotypes. Pathways enriched for differentially expressed genes were identified using the extensive MetaCore® database and include; non-canonical Wnt signaling and regulation of cytoskeleton remodeling (shortened axis and open blastopore), regulation by Yin Yang 1 (heart defects), Twist/Snail regulation of the epithelial to mesenchymal transition (open blastopore and neural tube), and Ets-1 regulation of transcription factors E2F1/E2F4 (heart defects and open blastopore). Advisors/Committee Members: Dr. Holly Goodson, Committee Member, Dr. Patricia Clark , Committee Member, Dr. Robert Schulz, Committee Member.

Subjects/Keywords: microarray; 5S ribosomal RNA; transcriptional regulation; development; Xenopus laevis; Sumoylation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bertke, M. M. (2014). The Role of Sumoylation in Early Development of Xenopus laevis and Regulation of 5S Ribosomal RNA Genes</h1>. (Thesis). University of Notre Dame. Retrieved from https://curate.nd.edu/show/zw12z31988j

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Bertke, Michelle Marie. “The Role of Sumoylation in Early Development of Xenopus laevis and Regulation of 5S Ribosomal RNA Genes</h1>.” 2014. Thesis, University of Notre Dame. Accessed April 17, 2021. https://curate.nd.edu/show/zw12z31988j.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Bertke, Michelle Marie. “The Role of Sumoylation in Early Development of Xenopus laevis and Regulation of 5S Ribosomal RNA Genes</h1>.” 2014. Web. 17 Apr 2021.

Vancouver:

Bertke MM. The Role of Sumoylation in Early Development of Xenopus laevis and Regulation of 5S Ribosomal RNA Genes</h1>. [Internet] [Thesis]. University of Notre Dame; 2014. [cited 2021 Apr 17]. Available from: https://curate.nd.edu/show/zw12z31988j.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Bertke MM. The Role of Sumoylation in Early Development of Xenopus laevis and Regulation of 5S Ribosomal RNA Genes</h1>. [Thesis]. University of Notre Dame; 2014. Available from: https://curate.nd.edu/show/zw12z31988j

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Melbourne

16. Cameron, Donald Peter John. Investigating acquired resistance to Pol I transcription inhibitors for the treatment of haematologic malignancies.

Degree: 2018, University of Melbourne

URL: http://hdl.handle.net/11343/216032

► Previous work from our group and others has demonstrated that CX-5461 (Senhwa Biosciences), a first-in-class small molecule inhibitor of RNA Polymerase I transcription of the… (more)

▼ Previous work from our group and others has demonstrated that CX-5461 (Senhwa Biosciences), a first-in-class small molecule inhibitor of RNA Polymerase I transcription of the ribosomal RNA genes, is effective at treating a range of different cancers both in vitro and in vivo, and is currently in clinical trials for haematologic and solid tumours. However, despite initial tumour clearance in response to CX-5461 treatment in preclinical murine models of cancer, mice eventually relapse with tumours that are resistant to further CX-5461 treatment. This thesis investigates the mechanisms via which the tumours can develop resistance to CX-5461 treatment and extrapolates this research to better understand: 1) how CX-5461 functions as an anti-tumour agent; 2) which pathways are required to mediate resistance to CX-5461; and 3) how resistance can be overcome with combination therapy. Using DNA exome sequencing, we found that Top2α is frequently mutated in tumours that have acquired resistance to CX-5461 treatment in vivo. Functional characterization of a Top2α mutant cell line demonstrated that Top2α expression and activity were reduced in these cells. Indeed, we found that knockdown of Top2α was sufficient to cause resistance to CX-5461. This implies that Top2α could provide a novel biomarker for CX-5461 response in clinical trials. Further investigation of the CX-5461 resistance mechanism uncovered that CX-5461 also acts as a Top2 inhibitor in addition to its ability to inhibit rDNA transcription. However, unlike common chemotherapeutic Top2 inhibitors which kill cells by causing genome-wide DNA damage thereby initiating a DNA damage response, CX-5461 treatment causes comparatively fewer DNA breaks enriched at the ribosomal DNA promoter loci. Thus, CX-5461 is able to kill tumour cells via the DNA damage response in the absence of extensive DNA damage thereby potentially limiting the cytotoxicity of drug treatment. Together, the work presented in this thesis identifies novel mechanisms of action and resistance to CX-5461. We propose that CX-5461 and other second-generation inhibitors of RNA Polymerase I and Top2α may provide a viable, less genotoxic alternative to classic Top2 inhibitors.

Subjects/Keywords: topoisomerase; ribosomal DNA; RNA Polymerase I; cancer; drug resistance; DNA damage

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APA (6^th Edition):

Cameron, D. P. J. (2018). Investigating acquired resistance to Pol I transcription inhibitors for the treatment of haematologic malignancies. (Doctoral Dissertation). University of Melbourne. Retrieved from http://hdl.handle.net/11343/216032

Chicago Manual of Style (16^th Edition):

Cameron, Donald Peter John. “Investigating acquired resistance to Pol I transcription inhibitors for the treatment of haematologic malignancies.” 2018. Doctoral Dissertation, University of Melbourne. Accessed April 17, 2021. http://hdl.handle.net/11343/216032.

MLA Handbook (7^th Edition):

Cameron, Donald Peter John. “Investigating acquired resistance to Pol I transcription inhibitors for the treatment of haematologic malignancies.” 2018. Web. 17 Apr 2021.

Vancouver:

Cameron DPJ. Investigating acquired resistance to Pol I transcription inhibitors for the treatment of haematologic malignancies. [Internet] [Doctoral dissertation]. University of Melbourne; 2018. [cited 2021 Apr 17]. Available from: http://hdl.handle.net/11343/216032.

Council of Science Editors:

Cameron DPJ. Investigating acquired resistance to Pol I transcription inhibitors for the treatment of haematologic malignancies. [Doctoral Dissertation]. University of Melbourne; 2018. Available from: http://hdl.handle.net/11343/216032

Laurentian University

17. Mispel-Beyer, Kyle. The effect of chemotherapy treatment on Ribosomal RNA integrity and ribosomal protein composition in ovarian cancer cells .

Degree: 2017, Laurentian University

URL: https://zone.biblio.laurentian.ca/handle/10219/2707

► Recently we have demonstrated that several chemotherapy agents, of distinct mechanisms, promote highly reproducible ribosomal RNA (rRNA) degradation patterns, a phenomenon we call RNA disruption.… (more)

Subjects/Keywords: chemotherapy; ribosomal RNA; rRNA; protein; ovarian cancer; cells

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APA (6^th Edition):

Mispel-Beyer, K. (2017). The effect of chemotherapy treatment on Ribosomal RNA integrity and ribosomal protein composition in ovarian cancer cells . (Thesis). Laurentian University. Retrieved from https://zone.biblio.laurentian.ca/handle/10219/2707

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mispel-Beyer, Kyle. “The effect of chemotherapy treatment on Ribosomal RNA integrity and ribosomal protein composition in ovarian cancer cells .” 2017. Thesis, Laurentian University. Accessed April 17, 2021. https://zone.biblio.laurentian.ca/handle/10219/2707.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mispel-Beyer, Kyle. “The effect of chemotherapy treatment on Ribosomal RNA integrity and ribosomal protein composition in ovarian cancer cells .” 2017. Web. 17 Apr 2021.

Vancouver:

Mispel-Beyer K. The effect of chemotherapy treatment on Ribosomal RNA integrity and ribosomal protein composition in ovarian cancer cells . [Internet] [Thesis]. Laurentian University; 2017. [cited 2021 Apr 17]. Available from: https://zone.biblio.laurentian.ca/handle/10219/2707.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mispel-Beyer K. The effect of chemotherapy treatment on Ribosomal RNA integrity and ribosomal protein composition in ovarian cancer cells . [Thesis]. Laurentian University; 2017. Available from: https://zone.biblio.laurentian.ca/handle/10219/2707

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Montana

18. Hargreaves, Melissa Lynn. Novel Ribosome Biogenesis in the Lyme Disease Spirochete Borrelia burgdorferi.

Degree: PhD, 2013, University of Montana

URL: https://scholarworks.umt.edu/etd/711

► Here we demonstrate the first characterization of an RNase III enzyme from a spirochete and its role in processing rRNA transcripts from the unusual rRNA… (more)

▼ Here we demonstrate the first characterization of an RNase III enzyme from a spirochete and its role in processing rRNA transcripts from the unusual rRNA gene operons of Borrelia burgdorferi. In most bacteria, the three rRNA transcripts (16S, 23S, and 5S rRNAs) that form the ribosome are produced as a single transcript from an operon with minimal spacing between genes. In the B. burgdorferi genome, however, a single 16S rRNA gene is encoded more than 3 kb from the bicistronic 23S-5S rRNA operons. The 23S-5S operons are tandemly duplicated, yielding an uneven number of rRNA genes, a feature unique to Lyme disease Borrelia. Additionally, the 16S and tandem 23S-5S operons appear to be synthesized as two separate transcripts. Our data show that B. burgdorferi RNase III processes the 3´ end of the 16S, 23S, but not the 5S, rRNA transcripts, as in other bacteria. However, 16S rRNA 5´ end processing proceeds by an as yet unidentified mechanism, which is an unprecedented finding. We hypothesize that this deviation from the canonical 16S rRNA processing pathway is likely an adaptation of B. burgdorferi to rRNA gene rearrangement during genome reduction and transition to a host-restricted lifestyle. In agreement with this finding, the 16S rRNA gene is transcribed as part of a larger operon containing unrelated genes, suggesting alternative regulation of the rRNA transcripts. Additionally, we show that the 23S rRNA is transcribed from identical promoters present in front of both tandem 23S rRNA genes and that this creates our observed 2.5 to 3-fold excess of 23S rRNA compared to 16S rRNA. Finally, single deletion mutants in each of the 23S rRNA genes were constructed. Surprisingly, deletion of the first 23S rRNA gene produces a severe growth phenotype and increased erythromycin susceptibility in vitro and a strain that is non-infectious in vivo. A mutant with a deletion in the second 23S rRNA gene shows no phenotype. The 23S rRNA genes have begun to acquire single nucleotide polymorphisms. However, their pattern currently indicates that they are the products of genetic drift. We conclude that the mechanism of rRNA transcription is unique in B. burgdorferi.

Subjects/Keywords: Borrelia burgdorferi; gene regulation; ribosomal RNA; ribosome biogenesis; spirochete

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APA (6^th Edition):

Hargreaves, M. L. (2013). Novel Ribosome Biogenesis in the Lyme Disease Spirochete Borrelia burgdorferi. (Doctoral Dissertation). University of Montana. Retrieved from https://scholarworks.umt.edu/etd/711

Chicago Manual of Style (16^th Edition):

Hargreaves, Melissa Lynn. “Novel Ribosome Biogenesis in the Lyme Disease Spirochete Borrelia burgdorferi.” 2013. Doctoral Dissertation, University of Montana. Accessed April 17, 2021. https://scholarworks.umt.edu/etd/711.

MLA Handbook (7^th Edition):

Hargreaves, Melissa Lynn. “Novel Ribosome Biogenesis in the Lyme Disease Spirochete Borrelia burgdorferi.” 2013. Web. 17 Apr 2021.

Vancouver:

Hargreaves ML. Novel Ribosome Biogenesis in the Lyme Disease Spirochete Borrelia burgdorferi. [Internet] [Doctoral dissertation]. University of Montana; 2013. [cited 2021 Apr 17]. Available from: https://scholarworks.umt.edu/etd/711.

Council of Science Editors:

Hargreaves ML. Novel Ribosome Biogenesis in the Lyme Disease Spirochete Borrelia burgdorferi. [Doctoral Dissertation]. University of Montana; 2013. Available from: https://scholarworks.umt.edu/etd/711

University of Melbourne

19. Quin, Jaclyn. Investigating the p53-independent responses to inhibition of RNA Polymerase I transcription by CX-5461.

Degree: 2017, University of Melbourne

URL: http://hdl.handle.net/11343/213474

► Increased rates of DNA-dependent RNA Polymerase I (Pol I) transcription of the 47S pre-ribosomal RNA (rRNA) genes are observed in almost all cancer types. Cancer… (more)

▼ Increased rates of DNA-dependent RNA Polymerase I (Pol I) transcription of the 47S pre-ribosomal RNA (rRNA) genes are observed in almost all cancer types. Cancer cells may require high rates of Pol I transcription and ribosome biogenesis to achieve their unrestrained growth and proliferative capacity, thus presenting a therapeutic window for selectively targeting cancer cells with inhibitors of Pol I transcription. Our laboratory helped develop and validate a first-in-class small molecule selective inhibitor of Pol I transcription, CX-5461 (Senhwa Biosciences). Here, we have investigated the response of cells at defined stages of malignant transformation to inhibition of Pol I transcription, utilising a panel of isogenically matched BJ fibroblast cell lines. We compared the response of non-transformed and transformed cells of the same genetic background, and demonstrated that CX-5461 can selectively induce cell death in cancer cell lines in vitro. We investigated the phenotypic response of a nontransformed BJ fibroblast cell line minimally immortalized with hTERT (BJ-T) to CX- 5461, and demonstrated that they display a proliferation defect. The proliferation defect is associated with the activation of p53 and a p53-dependent G1 cell cycle checkpoint, as well as p53-independent S-phase and G2 cell cycle checkpoints and senescence. Escape from cell cycle arrest in transformed BJ fibroblast cell lines is associated with increased rates of cell death in response to CX-5461. To identify pathways mediating the p53-independent responses to inhibition of Pol I transcription, we have performed RNA-sequencing analysis in CX-5461 treated BJ-T cells in which p53 was silenced (BJ-T p53shRNA). The analysis identified ATM (Ataxia-telangiectasia mutated) / ATR (ATM and RAD3-related) signaling and transcriptional programs associated with senescence to be modulated following treatment with CX-5461. Further, we have demonstrated that inhibition of Pol I transcription by CX-5461 rapidly and potently activates the ATM/ATR kinase signaling pathways in the absence of global DNA damage. Combined ATM/ATR inhibition and CX-5461 treatment results in bypass of CX-5461 mediated S-phase and G2 arrest, and induced cell death in the BJ-T p53shRNA cell line. We investigated the mechanisms by which inhibition of Pol I transcription by CX-5461 activates the ATM/ATR signaling pathways. We demonstrated that inhibition of Pol I transcription initiation by CX-5461 results in ‘exposed’ rRNA genes (rDNA) that are in an open chromatin conformation but devoid of Pol I. Inhibition of Pol I transcription by CX-5461 also results in reorganization of nucleolar structure and translocation of proteins to and from the nucleoli. We observed increased levels of NBS1 (Nijmegen Breakage Syndrome 1) activation by ATM specifically within the nucleoli during S/G2. We propose CX-5461 treatment induces an unusual chromatin structure at the rDNA that is sufficient to activate ATM/ATR in the nucleoli. Finally, we have shown that DNA damage repair is attenuated…

Subjects/Keywords: ribosome; ribosomal RNA; rRNA; ribosomal RNA genes; rDNA; RNA Polymerase I; Pol I; CX-5461; cancer; nucleoli; nucleolar stress signaling; p53; DNA damage response; DDR; ATM; ATR

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APA (6^th Edition):

Quin, J. (2017). Investigating the p53-independent responses to inhibition of RNA Polymerase I transcription by CX-5461. (Doctoral Dissertation). University of Melbourne. Retrieved from http://hdl.handle.net/11343/213474

Chicago Manual of Style (16^th Edition):

Quin, Jaclyn. “Investigating the p53-independent responses to inhibition of RNA Polymerase I transcription by CX-5461.” 2017. Doctoral Dissertation, University of Melbourne. Accessed April 17, 2021. http://hdl.handle.net/11343/213474.

MLA Handbook (7^th Edition):

Quin, Jaclyn. “Investigating the p53-independent responses to inhibition of RNA Polymerase I transcription by CX-5461.” 2017. Web. 17 Apr 2021.

Vancouver:

Quin J. Investigating the p53-independent responses to inhibition of RNA Polymerase I transcription by CX-5461. [Internet] [Doctoral dissertation]. University of Melbourne; 2017. [cited 2021 Apr 17]. Available from: http://hdl.handle.net/11343/213474.

Council of Science Editors:

Quin J. Investigating the p53-independent responses to inhibition of RNA Polymerase I transcription by CX-5461. [Doctoral Dissertation]. University of Melbourne; 2017. Available from: http://hdl.handle.net/11343/213474

University of Saskatchewan

20. Wakely, Heather. Binding characteristics and localization of Arabidopsis thaliana ribosomal protein S15a isoforms.

Degree: 2008, University of Saskatchewan

URL: http://hdl.handle.net/10388/etd-11032008-154759

► Ribosomes which conduct protein synthesis in all living organisms are comprised of two subunits. The large 60S ribosomal subunit catalyzes peptidyl transferase reactions and includes… (more)

Subjects/Keywords: RNA binding; ribosomal RNA; ribosome; ribosomal proteins; plant molecular biology

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APA (6^th Edition):

Wakely, H. (2008). Binding characteristics and localization of Arabidopsis thaliana ribosomal protein S15a isoforms. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/etd-11032008-154759

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Wakely, Heather. “Binding characteristics and localization of Arabidopsis thaliana ribosomal protein S15a isoforms.” 2008. Thesis, University of Saskatchewan. Accessed April 17, 2021. http://hdl.handle.net/10388/etd-11032008-154759.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Wakely, Heather. “Binding characteristics and localization of Arabidopsis thaliana ribosomal protein S15a isoforms.” 2008. Web. 17 Apr 2021.

Vancouver:

Wakely H. Binding characteristics and localization of Arabidopsis thaliana ribosomal protein S15a isoforms. [Internet] [Thesis]. University of Saskatchewan; 2008. [cited 2021 Apr 17]. Available from: http://hdl.handle.net/10388/etd-11032008-154759.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Wakely H. Binding characteristics and localization of Arabidopsis thaliana ribosomal protein S15a isoforms. [Thesis]. University of Saskatchewan; 2008. Available from: http://hdl.handle.net/10388/etd-11032008-154759

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

NSYSU

21. Yang, Ya-Wen. Polymorphic symbiosis and phylogenetic analysis of zooxanthellae in the Indo- Pacific scleractinian corals.

Degree: Master, Marine Biology, 2001, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0724101-150417

► Zooxanthellae are very important for the coral reef ecosystem. The diversity of coral hosts is high in the Indo-Pacific, but the diversity of zooxanthellae has… (more)

▼ Zooxanthellae are very important for the coral reef ecosystem. The diversity of coral hosts is high in the Indo-Pacific, but the diversity of zooxanthellae has not been broadly investigated. Southern Taiwan and Penghu Islands are coral reef and non-reefal communities, respectively. These localities were chosen as the sampling sites for this study to maximize the opportunity of surveying this region in the Indo-Pacific. Zooxanthellae diversity was investigated in 40 host species including 32 species of Scleractinia, 4 species of Actiniaria, 3 species of Milleporina and 1 species of Helioporacea using polymerase chain reaction (PCR) of the ssrRNA gene and restriction fragment length polymorphism (RFLP) patterns. The phylogenetic relationship of partial and complete sequences of the ssrRNA gene were also analysed. Aiptasia puchella harbors clade B; Oulastrea crispata only harbors clade E; while Acropora palifera and Montipora cactus harbor both clades C and E. Zooxanthellae isolated from all except the above 4 host species are identified as "clade C" sensu Rowan and Powers (1991a). Therefore, the clade C is the dominant type in the Indo-Pacific. Phylogenetic analyses based on partial and complete sequences obtained in this study and also from the GenBank data base demonstrate 4 clades (A, B, C and E) in the genus Symbiodinium. Clade E, classed as D3 RFLP type in previous studies, is a distinct clade differing from A, B and C by RFLP and sequencing data. Clade E has only been found in Scleractinia host species collected in shallow-water habitats in the Pacific. The composition of zooxanthellae clades and ecological pattern of polymorphic symbiosis is not consistent with the irradiance adaptation hypothesis in the Caribbean. A literature survey of zooxanthellae in Scleractinian hosts indicates a significant difference between the Caribbean and the Pacific. The documented biogeography of zooxanthellae clades and the ecological pattern of polymorphic symbiosis are also differ between the Caribbean and the Indo-Pacific. Advisors/Committee Members: Tse-Min Lee (committee member), J. T. Wang (chair), Allen C. Chen (committee member).

Subjects/Keywords: small-subunit ribosomal RNA gene; Symbiodinium

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APA (6^th Edition):

Yang, Y. (2001). Polymorphic symbiosis and phylogenetic analysis of zooxanthellae in the Indo- Pacific scleractinian corals. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0724101-150417

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Yang, Ya-Wen. “Polymorphic symbiosis and phylogenetic analysis of zooxanthellae in the Indo- Pacific scleractinian corals.” 2001. Thesis, NSYSU. Accessed April 17, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0724101-150417.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Yang, Ya-Wen. “Polymorphic symbiosis and phylogenetic analysis of zooxanthellae in the Indo- Pacific scleractinian corals.” 2001. Web. 17 Apr 2021.

Vancouver:

Yang Y. Polymorphic symbiosis and phylogenetic analysis of zooxanthellae in the Indo- Pacific scleractinian corals. [Internet] [Thesis]. NSYSU; 2001. [cited 2021 Apr 17]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0724101-150417.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Yang Y. Polymorphic symbiosis and phylogenetic analysis of zooxanthellae in the Indo- Pacific scleractinian corals. [Thesis]. NSYSU; 2001. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0724101-150417

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

22. Campbell, Alexandra Mandina. Prokaryotic Diversity of the Wastewater Outfalls, Reefs, and Inlets of Broward County.

Degree: 2014, Nova Southeastern University

URL: http://nsuworks.nova.edu/occ_stuetd/7

► We applied culture-independent, next-generation sequencing (NGS) high throughput pyrosequencing, to characterize the microbial communities associated with near shore seawater in Broward County, FL. These waters… (more)

Subjects/Keywords: 16S; Ribosomal RNA; Bacteria; Archaea; Pyrosequencing; Seawater; Outfalls; Inlets; Reefs; Marine Biology

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APA (6^th Edition):

Campbell, A. M. (2014). Prokaryotic Diversity of the Wastewater Outfalls, Reefs, and Inlets of Broward County. (Thesis). Nova Southeastern University. Retrieved from http://nsuworks.nova.edu/occ_stuetd/7

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Campbell, Alexandra Mandina. “Prokaryotic Diversity of the Wastewater Outfalls, Reefs, and Inlets of Broward County.” 2014. Thesis, Nova Southeastern University. Accessed April 17, 2021. http://nsuworks.nova.edu/occ_stuetd/7.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Campbell, Alexandra Mandina. “Prokaryotic Diversity of the Wastewater Outfalls, Reefs, and Inlets of Broward County.” 2014. Web. 17 Apr 2021.

Vancouver:

Campbell AM. Prokaryotic Diversity of the Wastewater Outfalls, Reefs, and Inlets of Broward County. [Internet] [Thesis]. Nova Southeastern University; 2014. [cited 2021 Apr 17]. Available from: http://nsuworks.nova.edu/occ_stuetd/7.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Campbell AM. Prokaryotic Diversity of the Wastewater Outfalls, Reefs, and Inlets of Broward County. [Thesis]. Nova Southeastern University; 2014. Available from: http://nsuworks.nova.edu/occ_stuetd/7

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Michigan

23. Lund, Paul E. Interactions between the Translation Machinery and a Translational preQ1 Riboswitch.

Degree: PhD, Chemical Biology, 2015, University of Michigan

URL: http://hdl.handle.net/2027.42/116677

► Gene expression is highly regulated with a diversity of regulation at the RNA level. In bacteria, regulation of mRNA translation into protein often occurs through… (more)

▼ Gene expression is highly regulated with a diversity of regulation at the RNA level. In bacteria, regulation of mRNA translation into protein often occurs through RNA sequence features such as the Shine-Dalgarno (SD) sequence and local structural features. Translational riboswitches in bacteria exemplify such cis-acting regulation. This work look at how structural features of a preQ1 riboswitch effect regulation through interactions with the translation machinery. Broader questions about how individual translational machinery components, such as ribosomal protein S1 and the 30S ribosomal subunit, interact with structured RNAs are also addressed. We sought a more detailed mechanistic view of the interplay between the translational preQ1 riboswitch found in the 5′ UTR of an mRNA from T. tengcongensis, its ligand preQ1, and the SD sequence accessibility. To this end, we developed SiM-KARTS, a generalized strategy to interrogate site-specific structural dynamics of RNA molecules based on probe hybridization kinetics. Intriguingly, we found that the riboswitch expression platform alternates between conformations with differing SD accessibility, which are distinguished by “bursts” of probe binding, the pattern of which is modulated by ligand. This challenges the assumption that riboswitches behave in simple ON/OFF fashion and thus has broader implications for how we think about translational riboswitch regulation. The folding and unfolding of RNA structure influences other cellular processes besides translation. Ribosomal protein S1 performs other roles outside of the context of translation, which are related to its RNA binding or unfolding capacity. We used the well-characterized preQ1 riboswitch as a model pseudoknot to study how S1 interacts with defined, stable tertiary structure. S1 is able to bind and at least partially unfold this pseudoknot in a manner that is limited by RNA structural stability. Lastly, we investigated the influence of S1 on translation of preQ1 riboswitch-containing mRNAs and found that the effects of ligand on translation are not potentiated by the loss of S1. There is, however, a dramatic effect on translational coupling, invoking a role for S1 in polycistronic mRNA translation. These results highlight the need for additional techniques, such as assays at the single molecule level, to monitor early 30S-mRNA interactions during translation. Advisors/Committee Members: Walter, Nils G. (committee member), Fierke, Carol A (committee member), Maddock, Janine R (committee member), O'Brien, Patrick (committee member).

Subjects/Keywords: preQ1 riboswitch; ribosomal protein S1; ribosome translation; SiM-KARTS; RNA structure; Biological Chemistry; Chemistry; Science

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APA (6^th Edition):

Lund, P. E. (2015). Interactions between the Translation Machinery and a Translational preQ1 Riboswitch. (Doctoral Dissertation). University of Michigan. Retrieved from http://hdl.handle.net/2027.42/116677

Chicago Manual of Style (16^th Edition):

Lund, Paul E. “Interactions between the Translation Machinery and a Translational preQ1 Riboswitch.” 2015. Doctoral Dissertation, University of Michigan. Accessed April 17, 2021. http://hdl.handle.net/2027.42/116677.

MLA Handbook (7^th Edition):

Lund, Paul E. “Interactions between the Translation Machinery and a Translational preQ1 Riboswitch.” 2015. Web. 17 Apr 2021.

Vancouver:

Lund PE. Interactions between the Translation Machinery and a Translational preQ1 Riboswitch. [Internet] [Doctoral dissertation]. University of Michigan; 2015. [cited 2021 Apr 17]. Available from: http://hdl.handle.net/2027.42/116677.

Council of Science Editors:

Lund PE. Interactions between the Translation Machinery and a Translational preQ1 Riboswitch. [Doctoral Dissertation]. University of Michigan; 2015. Available from: http://hdl.handle.net/2027.42/116677

University of Ottawa

24. Peters, Melissa. Genetic and Genome Analyses of Native Populations of the Honeybee Pathogen Nosema ceranae .

Degree: 2018, University of Ottawa

URL: http://hdl.handle.net/10393/38049

► Microsporidia are a unique phylum of ubiquitous fungal pathogens that are able to infect a wide variety of hosts, including economically and ecologically important organisms.… (more)

Subjects/Keywords: Microsporidia; Nosema ceranae; Honeybees; Small ribosomal subunit RNA gene; Next-Generation sequencing; genome diversity

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APA (6^th Edition):

Peters, M. (2018). Genetic and Genome Analyses of Native Populations of the Honeybee Pathogen Nosema ceranae . (Thesis). University of Ottawa. Retrieved from http://hdl.handle.net/10393/38049

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Peters, Melissa. “Genetic and Genome Analyses of Native Populations of the Honeybee Pathogen Nosema ceranae .” 2018. Thesis, University of Ottawa. Accessed April 17, 2021. http://hdl.handle.net/10393/38049.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Peters, Melissa. “Genetic and Genome Analyses of Native Populations of the Honeybee Pathogen Nosema ceranae .” 2018. Web. 17 Apr 2021.

Vancouver:

Peters M. Genetic and Genome Analyses of Native Populations of the Honeybee Pathogen Nosema ceranae . [Internet] [Thesis]. University of Ottawa; 2018. [cited 2021 Apr 17]. Available from: http://hdl.handle.net/10393/38049.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Peters M. Genetic and Genome Analyses of Native Populations of the Honeybee Pathogen Nosema ceranae . [Thesis]. University of Ottawa; 2018. Available from: http://hdl.handle.net/10393/38049

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Hawaii – Manoa

25. Jungbluth, Sean Patrick. Microbial ecology in the sediment-covered ocean basement of the Juan de Fuca Ridge.

Degree: 2015, University of Hawaii – Manoa

URL: http://hdl.handle.net/10125/101095

►

Ph.D. University of Hawaii at Manoa 2014.

Investigations of microbial life inside of the deep seafloor and most reviews on the topic have focused on… (more)

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Ph.D. University of Hawaii at Manoa 2014.

Investigations of microbial life inside of the deep seafloor and most reviews on the topic have focused on sediments and largely ignore the prospect of a biosphere inside the basaltic crust underlying the global system of ocean basins. This is despite the potential global importance of biogeochemical cycling that may be occurring in situ within the uppermost igneous ocean crust; a location that is predicted to be one of the most habitable subsurface environments due to its porosity, hydrothermal circulation, and expected chemical disequilibria. Sedimentation processes occuring over geologic time scales cause a majority of the global seafloor to be covered by thick and relatively impermeable blankets that prevent access to the underlying basaltic seafloor. As a result, studies of microorganisms inside the basaltic crust have traditionally been restricted to the exposed seafloor or to locations where hydrothermal fluids exiting the seafloor act as "windows" into the subsurface. However, these traditional methods for observing basaltic rock seafloor microorganisms are inadequate because ocean crust is hydrogeologically active until up to ~65 million years old and a majority of flow is likely to occur over long time scales and deep within the sediment-covered basement. Seafloor observatories that penetrate through sediments and into basement rock provide the infrastructure needed to collect samples from one of the planet's most remote environments. The broad goals of this study were to estimate the concentrations of microbial biomass and explore the microbial diversity in anoxic, deep subseafloor crustal fluids. Building on the first characterizations of microbial life in the aging ocean basement, discrete fluid samples were collected and analysed here from new borehole observatories that are the first to incorporate dedicated stainless steel or Teflon-coated fluid delivery lines running along the exterior of the reactive iron casing. Biofouling-resistant materials used during the construction of the seafloor observatory fluid delivery lines permit collection of pristine samples that can be used for estimation of the in situ microbial biomass and reveal a range of cellular abundances that are, on average, roughly an order of magnitude lower that those found in bottom seawater. The cellular abundances reported here will help to constrain estimates of biomass inside the global seafloor and elucidate partitioning between the basaltic crustal and marine sediment communities, and furthermore, underscore the difficulties associated with collecting uncontaminated samples from the deep subsurface. Sampling from a combination of older and newer borehole observatories has revealed novel microbial diversity and community structure from the seafloor that is distinct from overlying sediments and varies with the alteration state of the basement fluids. Microorganisms detected were largely from uncultivated groups, which means one can only speculate about the metabolic lifestyle for…

Subjects/Keywords: deep subsurface; marine microorganisms; diversity; Juan de Fuca Ridge; SSU ribosomal RNA gene; basement biosphere

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APA (6^th Edition):

Jungbluth, S. P. (2015). Microbial ecology in the sediment-covered ocean basement of the Juan de Fuca Ridge. (Thesis). University of Hawaii – Manoa. Retrieved from http://hdl.handle.net/10125/101095

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Jungbluth, Sean Patrick. “Microbial ecology in the sediment-covered ocean basement of the Juan de Fuca Ridge.” 2015. Thesis, University of Hawaii – Manoa. Accessed April 17, 2021. http://hdl.handle.net/10125/101095.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Jungbluth, Sean Patrick. “Microbial ecology in the sediment-covered ocean basement of the Juan de Fuca Ridge.” 2015. Web. 17 Apr 2021.

Vancouver:

Jungbluth SP. Microbial ecology in the sediment-covered ocean basement of the Juan de Fuca Ridge. [Internet] [Thesis]. University of Hawaii – Manoa; 2015. [cited 2021 Apr 17]. Available from: http://hdl.handle.net/10125/101095.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Jungbluth SP. Microbial ecology in the sediment-covered ocean basement of the Juan de Fuca Ridge. [Thesis]. University of Hawaii – Manoa; 2015. Available from: http://hdl.handle.net/10125/101095

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Duke University

26. Mao, Hanqian. Posttranscriptional Regulation of Embryonic Neurogenesis by the Exon Junction Complex .

Degree: 2016, Duke University

URL: http://hdl.handle.net/10161/12296

► The six-layered neuron structure in the cerebral cortex is the foundation for human mental abilities. In the developing cerebral cortex, neural stem cells undergo… (more)

▼ The six-layered neuron structure in the cerebral cortex is the foundation for human mental abilities. In the developing cerebral cortex, neural stem cells undergo proliferation and differentiate into intermediate progenitors and neurons, a process known as embryonic neurogenesis. Disrupted embryonic neurogenesis is the root cause of a wide range of neurodevelopmental disorders, including microcephaly and intellectual disabilities. Multiple layers of regulatory networks have been identified and extensively studied over the past decades to understand this complex but extremely crucial process of brain development. In recent years, post-transcriptional RNA regulation through RNA binding proteins has emerged as a critical regulatory nexus in embryonic neurogenesis. The exon junction complex (EJC) is a highly conserved RNA binding complex composed of four core proteins, Magoh, Rbm8a, Eif4a3, and Casc3. The EJC plays a major role in regulating RNA splicing, nuclear export, subcellular localization, translation, and nonsense mediated RNA decay. Human genetic studies have associated individual EJC components with various developmental disorders. We showed previously that haploinsufficiency of Magoh causes microcephaly and disrupted neural stem cell differentiation in mouse. However, it is unclear if other EJC core components are also required for embryonic neurogenesis. More importantly, the molecular mechanism through which the EJC regulates embryonic neurogenesis remains largely unknown. Here, we demonstrated with genetically modified mouse models that both Rbm8a and Eif4a3 are required for proper embryonic neurogenesis and the formation of a normal brain. Using transcriptome and proteomic analysis, we showed that the EJC posttranscriptionally regulates genes involved in the p53 pathway, splicing and translation regulation, as well as ribosomal biogenesis. This is the first in vivo evidence suggesting that the etiology of EJC associated neurodevelopmental diseases can be ribosomopathies. We also showed that, different from other EJC core components, depletion of Casc3 only led to mild neurogenesis defects in the mouse model. However, our data suggested that Casc3 is required for embryo viability, development progression, and is potentially a regulator of cardiac development. Together, data presented in this thesis suggests that the EJC is crucial for embryonic neurogenesis and that the EJC and its peripheral factors may regulate development in a tissue-specific manner. Advisors/Committee Members: Silver, Debra L (advisor).

Subjects/Keywords: Molecular biology; Developmental biology; Cellular biology; Exon Junction Complex; Posttranscriptional regulation; ribosomal biogenesis; RNA; Splicing

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mao, H. (2016). Posttranscriptional Regulation of Embryonic Neurogenesis by the Exon Junction Complex . (Thesis). Duke University. Retrieved from http://hdl.handle.net/10161/12296

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mao, Hanqian. “Posttranscriptional Regulation of Embryonic Neurogenesis by the Exon Junction Complex .” 2016. Thesis, Duke University. Accessed April 17, 2021. http://hdl.handle.net/10161/12296.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mao, Hanqian. “Posttranscriptional Regulation of Embryonic Neurogenesis by the Exon Junction Complex .” 2016. Web. 17 Apr 2021.

Vancouver:

Mao H. Posttranscriptional Regulation of Embryonic Neurogenesis by the Exon Junction Complex . [Internet] [Thesis]. Duke University; 2016. [cited 2021 Apr 17]. Available from: http://hdl.handle.net/10161/12296.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mao H. Posttranscriptional Regulation of Embryonic Neurogenesis by the Exon Junction Complex . [Thesis]. Duke University; 2016. Available from: http://hdl.handle.net/10161/12296

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Vienna

27. Lopez, Martin Alexander. Analysis of RNA chaperone activity of truncated ribosomal protein S12 from E.coli and of eukaryal and archaeal S12 orthologues.

Degree: 2010, University of Vienna

URL: http://othes.univie.ac.at/11041/

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RNA Moleküle sind sowohl strukturell als auch funktionell sehr flexibel. Zum Erlangen ihrer nativen Konformation benötigen sie daher die Hilfe von RNA Chaperonen. RNA Chaperone… (more)

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RNA Moleküle sind sowohl strukturell als auch funktionell sehr flexibel. Zum Erlangen ihrer nativen Konformation benötigen sie daher die Hilfe von RNA Chaperonen. RNA Chaperone sind Proteine die missgefaltete RNA Strukturen reparieren können bzw. die Missfaltung von RNA Molekülen verhindern. Die meisten Chaperone besitzen unstrukturierte Domänen oder sind in manchen Fällen komplett entfaltet. Theorien besagen, dass erst diese unstrukturierten Domänen die RNA Chaperon Aktivität der Proteine ermöglicht. Einige der bekanntesten und best konserviertesten RNA Chaperone sind ribosomale Proteine. Die ribosomalen Proteine werden einerseits für den Zusammenbau der Ribosomen benötigt, helfen, unter anderem, beim dekodieren der mRNA und besitzen auch extraribosomale Funktionen. Fast alle ribosomalen Proteine besitzen unstrukturierte Regionen. Eines der extremsten Beispiele ist das ribosomale Protein S12, das eine lange N-terminale unstrukturierte Domäne besitzt die sich durch die kleine ribosomale Untereinheit schlängelt. Weiters ist bekannt dass Escherichia coli S12 (Eco S12) ein RNA Chaperon ist. In dieser Diplomarbeit wird untersucht ob Eco S12 auch dann noch RNA Chaperonaktivität besitzt wenn seine lange unstrukturierte Domäne entfernt wird. Weiters wird getestet ob auch S12 Proteine aus anderen Organismen Chaperonaktivität besitzen. Um dies zu untersuchen mußten die verschiedenen S12 Gene kloniert werden, die Proteine aufgereinigt werden und die Chaperonaktivität wurde mittels Hammerhead Assay getestet. Die erzielten Resultate lassen erkennen, dass die unstrukturierte Domäne für die RNA Chaperonaktivität essentiell ist und dass auch die getesteten eukaryotischen Proteine RNA Chaperonaktivität besitzen, nicht aber ein aus einem thermophilen Archaea isoliertes S12 Protein. Weiters wird die RNA Chaperonaktivität des ribosomalen Proteins L19 mit einer in vivo Methode untersucht. Dabei wird dieser Versuch erstmals mit Fluoreszenz markierten Proben durchgeführt. Zudem wurde noch die RNA Chaperonaktivität von rekombinanten L1 Proteinen in vitro und von L1 Orthologen in vivo untersucht.

RNA molecules are structurally and functionally very flexible. As a result, they require the assistance of RNA chaperones to reach their ultimate folding state. RNA chaperones are proteins which can resolve misfolded RNA structures or which prevent the formation of misfolded structures. Many RNA chaperones possess intrinsically unstructured domains or are even completely unstructured. Some theories claim that these unstructured domains are required for the RNA chaperone activity. Many ribosomal proteins possess strong RNA chaperone activity. In addition, they are highly conserved. Ribosomal proteins are required for the ribosome assembly, assist, among others, the decoding of the mRNA and also possess extraribosomal functions. The majority of the ribosomal proteins have intrinsically unstructured domains. One of the most impressive examples is the ribosomal protein S12. It has a long N-terminal extension which penetrates through the small…

Subjects/Keywords: 42.13 Molekularbiologie; RNA Chaperon Aktivität / S12 / ribosomale Proteine / unstrukturierte Domänen / L1; RNA chaperone activity / S12 / ribosomal proteins / unstructured domains / L1

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lopez, M. A. (2010). Analysis of RNA chaperone activity of truncated ribosomal protein S12 from E.coli and of eukaryal and archaeal S12 orthologues. (Thesis). University of Vienna. Retrieved from http://othes.univie.ac.at/11041/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lopez, Martin Alexander. “Analysis of RNA chaperone activity of truncated ribosomal protein S12 from E.coli and of eukaryal and archaeal S12 orthologues.” 2010. Thesis, University of Vienna. Accessed April 17, 2021. http://othes.univie.ac.at/11041/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lopez, Martin Alexander. “Analysis of RNA chaperone activity of truncated ribosomal protein S12 from E.coli and of eukaryal and archaeal S12 orthologues.” 2010. Web. 17 Apr 2021.

Vancouver:

Lopez MA. Analysis of RNA chaperone activity of truncated ribosomal protein S12 from E.coli and of eukaryal and archaeal S12 orthologues. [Internet] [Thesis]. University of Vienna; 2010. [cited 2021 Apr 17]. Available from: http://othes.univie.ac.at/11041/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lopez MA. Analysis of RNA chaperone activity of truncated ribosomal protein S12 from E.coli and of eukaryal and archaeal S12 orthologues. [Thesis]. University of Vienna; 2010. Available from: http://othes.univie.ac.at/11041/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Estadual de Campinas

28. Rodrigues, Débora Silva, 1986-. Estudo da organização do gene ribossomal 5S em populações de Engystomops da Amazônia (Anura, Leiuperidae).

Degree: Instituto de Biologia; Programa de Pós-Graduação em Biologia Celular e Estrutural, 2012, Universidade Estadual de Campinas

URL: RODRIGUES, Débora Silva. Estudo da organização do gene ribossomal 5S em populações de Engystomops da Amazônia (Anura, Leiuperidae). 2012. 54 f. Dissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Biologia, Campinas, SP. Disponível em: <http://www.repositorio.unicamp.br/handle/REPOSIP/317683>. Acesso em: 20 ago. 2018. ; http://repositorio.unicamp.br/jspui/handle/REPOSIP/317683

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Orientador: Luciana Bolsoni Lourenço Morandini

Dissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Biologia

Made available in DSpace on 2018-08-20T20:53:49Z (GMT). No. of bitstreams:… (more)

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Orientador: Luciana Bolsoni Lourenço Morandini

Dissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Biologia

Made available in DSpace on 2018-08-20T20:53:49Z (GMT). No. of bitstreams: 1 Rodrigues_DeboraSilva_M.pdf: 3296545 bytes, checksum: 2a2ccc454f9a1df9f09173c2127af330 (MD5) Previous issue date: 2012

Resumo: O gênero Engystomops apresenta ampla distribuição geográfica e constitui um interessante grupo de anuros para estudos cariotípicos. As populações de Engystomops encontradas na Amazônia têm sua identificação taxonômica ainda controversa. Análises genéticas e citogenéticas apoiam hipóteses que sugerem a existência de um complexo de espécies crípticas e especiação incipiente. Muitas vezes a variação citogenética observada entre diferentes populações estudadas dificultou o reconhecimento de homeologias cromossômicas entre os cariótipos. Uma caracterização cromossômica mais detalhada poderia auxiliar no possível reconhecimento de homeologias cromossômicas e, dessa forma, contribuir para o estudo dos processos envolvidos na divergência desses anuros. Já que o gene do DNAr 5S tem sido importante marcador genético e citogenético para estudos evolutivos e para a identificação e comparação de espécies em diversos grupos, no presente trabalho o DNAr 5S de Engystomops freibergi e de exemplares de Engystomops petersi de duas localidades Equatorianas (Puyo e Yasuní) foi estudado. Em todos os casos, dois tipos de DNAr 5S, facilmente diferenciados pelo tamanho e composição da sequência do seu espaçador não transcrito, foram isolados. A provável região promotora do gene do RNAr 5S (ICR) foi localizada nos dois tipos de sequências de DNAr 5S e a presença de possíveis sequências regulatórias adicionais foi discutida. No cariótipo de E. freibergi, sonda contendo a unidade repetitiva do DNAr 5S tipo I hibridou na região pericentromérica do braço curto dos cromossomos do par 3, e o DNAr 5S tipo II foi mapeado na região distal do braço longo dos cromossomos do par 6. A sonda formada somente pela região de NTS do DNAr 5S tipo I claramente detectou a região pericentromérica de 3p nos cariótipos de E. freibergi e E. petersi (Puyo) e de 5p no cariótipo de E. petersi (Yasuní), porém nenhum sinal distal ou intersticial foi observado. A sonda formada pela região de NTS do DNAr 5S tipo II detectou apenas a região distal de 6q nos três cariótipos estudados, corroborando a distribuição diferencial dos dois tipos de DNAr 5S nesses cariótipos. Tais sítios de DNAr 5S constituem novos marcadores cromossômicos, os quais permitem sugerir a homeologia entre o cromossomo 6 dos cariótipos de E. freibergi e de E. petersi, e entre o cromossomo 5 do cariótipo de E. petersi de Yasuní e o cromossomo 3 dos cariótipos de E freibergi e de E. petersi de Puyo. Já que os dois tipos de DNAr 5S encontrados em Engystomops são relacionados àqueles de Physalaemus tanto quanto à composição nucleotídica quanto à localização cromossômica, é ainda possível inferir que a origem desses dois tipos de sequências tenha antecedido a divergência…

Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS, Lourenço, Luciana Bolsoni, 1972-, Morandini, Luciana Bolsoni Lourenço, 1972-, Kasahara, Sanae, Martins, Cesar.

Subjects/Keywords: RNA ribossômico 5S; Gene NTS; Engystomops; Marcadores moleculares; Anfíbio - Genética; 5S ribosomal RNA; NTS gene; Engystomops; Molecular markers; Amphibians - Genetics

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Rodrigues, Débora Silva, 1. (2012). Estudo da organização do gene ribossomal 5S em populações de Engystomops da Amazônia (Anura, Leiuperidae). (Masters Thesis). Universidade Estadual de Campinas. Retrieved from RODRIGUES, Débora Silva. Estudo da organização do gene ribossomal 5S em populações de Engystomops da Amazônia (Anura, Leiuperidae). 2012. 54 f. Dissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Biologia, Campinas, SP. Disponível em: <http://www.repositorio.unicamp.br/handle/REPOSIP/317683>. Acesso em: 20 ago. 2018. ; http://repositorio.unicamp.br/jspui/handle/REPOSIP/317683

Chicago Manual of Style (16^th Edition):

Rodrigues, Débora Silva, 1986-. “Estudo da organização do gene ribossomal 5S em populações de Engystomops da Amazônia (Anura, Leiuperidae).” 2012. Masters Thesis, Universidade Estadual de Campinas. Accessed April 17, 2021. RODRIGUES, Débora Silva. Estudo da organização do gene ribossomal 5S em populações de Engystomops da Amazônia (Anura, Leiuperidae). 2012. 54 f. Dissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Biologia, Campinas, SP. Disponível em: <http://www.repositorio.unicamp.br/handle/REPOSIP/317683>. Acesso em: 20 ago. 2018. ; http://repositorio.unicamp.br/jspui/handle/REPOSIP/317683.

MLA Handbook (7^th Edition):

Rodrigues, Débora Silva, 1986-. “Estudo da organização do gene ribossomal 5S em populações de Engystomops da Amazônia (Anura, Leiuperidae).” 2012. Web. 17 Apr 2021.

Vancouver:

Rodrigues, Débora Silva 1. Estudo da organização do gene ribossomal 5S em populações de Engystomops da Amazônia (Anura, Leiuperidae). [Internet] [Masters thesis]. Universidade Estadual de Campinas; 2012. [cited 2021 Apr 17]. Available from: RODRIGUES, Débora Silva. Estudo da organização do gene ribossomal 5S em populações de Engystomops da Amazônia (Anura, Leiuperidae). 2012. 54 f. Dissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Biologia, Campinas, SP. Disponível em: <http://www.repositorio.unicamp.br/handle/REPOSIP/317683>. Acesso em: 20 ago. 2018. ; http://repositorio.unicamp.br/jspui/handle/REPOSIP/317683.

Council of Science Editors:

Rodrigues, Débora Silva 1. Estudo da organização do gene ribossomal 5S em populações de Engystomops da Amazônia (Anura, Leiuperidae). [Masters Thesis]. Universidade Estadual de Campinas; 2012. Available from: RODRIGUES, Débora Silva. Estudo da organização do gene ribossomal 5S em populações de Engystomops da Amazônia (Anura, Leiuperidae). 2012. 54 f. Dissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Biologia, Campinas, SP. Disponível em: <http://www.repositorio.unicamp.br/handle/REPOSIP/317683>. Acesso em: 20 ago. 2018. ; http://repositorio.unicamp.br/jspui/handle/REPOSIP/317683

Universidade Estadual de Campinas

29. Morello, Luis Gustavo, 1982-. Caracterização funcional das proteínas NIP7 e FTSJ3 no processamento do RNA ribossomal em células humanas.

Degree: Instituto de Biologia; Programa de Pós-Graduação em Genética e Biologia Molecular, 2012, Universidade Estadual de Campinas

URL: MORELLO, Luis Gustavo. Caracterização funcional das proteínas NIP7 e FTSJ3 no processamento do RNA ribossomal em células humanas. 2012. 136 p. Tese (doutorado) - Universidade Estadual de Campinas, Instituto de Biologia, Campinas, SP. Disponível em: <http://www.repositorio.unicamp.br/handle/REPOSIP/317176>. Acesso em: 20 ago. 2018. ; http://repositorio.unicamp.br/jspui/handle/REPOSIP/317176

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Orientador: Nilson Ivo Tonin Zanchin

Tese (doutorado) - Universidade Estadual de Campinas, Instituto de Biologia

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Orientador: Nilson Ivo Tonin Zanchin

Tese (doutorado) - Universidade Estadual de Campinas, Instituto de Biologia

Made available in DSpace on 2018-08-20T22:37:29Z (GMT). No. of bitstreams: 1 Morello_LuisGustavo_D.pdf: 80024877 bytes, checksum: 1848ac9901b4322b786b1dcd7a521a69 (MD5) Previous issue date: 2012

Resumo: Estudos prévios realizados em nosso laboratório demonstraram a interação entre as proteínas humanas SBDS e NIP7. SBDS participa da biogênese de ribossomos e sua deficiência está associada à síndrome de Shwachman- Bodian-Diamond. NIP7 é uma proteína conservada e já foi caracterizada em levedura, onde participa da formação da subunidade ribossomal 60S. Neste trabalho, nós investigamos o papel de NIP7 na síntese de ribossomos em células humanas. A depleção de NIP7 revelou defeitos no processamento do pré-rRNA associado à produção do rRNA 18S, causando déficit na formação da subunidade ribossomal 40S. Essa divergência de resultados entre a função de NIP7 em levedura e células humanas é consistente com o fato de que NIP7 humana não complementa levedura deficiente em Nip7p. Ainda, um rastreamento em sistema de duplo-híbrido tendo NIP7 humana como isca revelou parceiros de interação diferentes daqueles reportados para Nip7p em levedura. FTSJ3 foi a parceira isolada com maior frequência. FTSJ3 é a provável ortóloga de Spb1p em levedura, a qual está envolvida na formação da subunidade ribossomal 60S. A associação entre FTSJ3 e NIP7 foi demonstrada por ensaios de pull-down e imunoprecipitação, como sendo dependente de RNA. A co-localização nucleolar e co-sedimentação dessas proteínas em fracionamento em gradiente de sacarose corroboram a associação. Além disso, células humanas deficientes em FTSJ3 revelaram defeitos na via de maturação do rRNA 18S, mesma via afetada pela depleção de NIP7. Em adição, a caracterização proteômica de complexos contendo FTSJ3 e NIP7 revelaram que essas proteínas co-purificam complexos pré-ribossomais. Uma comparação entre o conjunto de proteínas que interagem com Spb1p e as proteínas identificadas nos ensaios de pull-down com FLAG-FTSJ3 revelou que elas apresentam apenas um ortólogo em comum, o qual, incrivelmente, é Nip7/NIP7. Essas observações revelaram diferenças significativas na função desses fatores durante a síntese de ribossomos em levedura e células humanas, adicionando NIP7 e FTSJ3 na lista crescente de fatores com funções divergentes nas vias de processamento do rRNA em levedura e humanos

Previous studies from our laboratory have demonstrated the interaction between the SBDS and NIP7 human proteins. SBDS play a role in ribosome biogenesis and its deficiency is associated to the Shwachman-Bodian-Diamond syndrome. NIP7 is a conserved protein and has already been characterized in yeast, where it participates in the 60S ribosomal subunit formation. In this work, we investigated the role of NIP7 in ribosome biogenesis in human cells. NIP7 knockdown caused pre-rRNA processing defects associated to the 18S rRNA maturation, leading to deficiency in 40S ribosomal subunit…

Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS, Zanchin, Nilson Ivo Tonin, 1962-, Gomes, Marcelo Damario, Araujo, Ana Paula Ulian de, Yunes, José Andrés, Benedetti, Celso Eduardo.

Subjects/Keywords: Ribossomos - Biossíntese; RNA ribossomico; Proteína NIP7; Proteína FTSJ3; Ribosomes - Biosynthesis; Ribosomal RNA; NIP7 protein; FTSJ3 protein

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Morello, Luis Gustavo, 1. (2012). Caracterização funcional das proteínas NIP7 e FTSJ3 no processamento do RNA ribossomal em células humanas. (Doctoral Dissertation). Universidade Estadual de Campinas. Retrieved from MORELLO, Luis Gustavo. Caracterização funcional das proteínas NIP7 e FTSJ3 no processamento do RNA ribossomal em células humanas. 2012. 136 p. Tese (doutorado) - Universidade Estadual de Campinas, Instituto de Biologia, Campinas, SP. Disponível em: <http://www.repositorio.unicamp.br/handle/REPOSIP/317176>. Acesso em: 20 ago. 2018. ; http://repositorio.unicamp.br/jspui/handle/REPOSIP/317176

Chicago Manual of Style (16^th Edition):

Morello, Luis Gustavo, 1982-. “Caracterização funcional das proteínas NIP7 e FTSJ3 no processamento do RNA ribossomal em células humanas.” 2012. Doctoral Dissertation, Universidade Estadual de Campinas. Accessed April 17, 2021. MORELLO, Luis Gustavo. Caracterização funcional das proteínas NIP7 e FTSJ3 no processamento do RNA ribossomal em células humanas. 2012. 136 p. Tese (doutorado) - Universidade Estadual de Campinas, Instituto de Biologia, Campinas, SP. Disponível em: <http://www.repositorio.unicamp.br/handle/REPOSIP/317176>. Acesso em: 20 ago. 2018. ; http://repositorio.unicamp.br/jspui/handle/REPOSIP/317176.

MLA Handbook (7^th Edition):

Morello, Luis Gustavo, 1982-. “Caracterização funcional das proteínas NIP7 e FTSJ3 no processamento do RNA ribossomal em células humanas.” 2012. Web. 17 Apr 2021.

Vancouver:

Morello, Luis Gustavo 1. Caracterização funcional das proteínas NIP7 e FTSJ3 no processamento do RNA ribossomal em células humanas. [Internet] [Doctoral dissertation]. Universidade Estadual de Campinas; 2012. [cited 2021 Apr 17]. Available from: MORELLO, Luis Gustavo. Caracterização funcional das proteínas NIP7 e FTSJ3 no processamento do RNA ribossomal em células humanas. 2012. 136 p. Tese (doutorado) - Universidade Estadual de Campinas, Instituto de Biologia, Campinas, SP. Disponível em: <http://www.repositorio.unicamp.br/handle/REPOSIP/317176>. Acesso em: 20 ago. 2018. ; http://repositorio.unicamp.br/jspui/handle/REPOSIP/317176.

Council of Science Editors:

Morello, Luis Gustavo 1. Caracterização funcional das proteínas NIP7 e FTSJ3 no processamento do RNA ribossomal em células humanas. [Doctoral Dissertation]. Universidade Estadual de Campinas; 2012. Available from: MORELLO, Luis Gustavo. Caracterização funcional das proteínas NIP7 e FTSJ3 no processamento do RNA ribossomal em células humanas. 2012. 136 p. Tese (doutorado) - Universidade Estadual de Campinas, Instituto de Biologia, Campinas, SP. Disponível em: <http://www.repositorio.unicamp.br/handle/REPOSIP/317176>. Acesso em: 20 ago. 2018. ; http://repositorio.unicamp.br/jspui/handle/REPOSIP/317176

Universidade Estadual de Campinas

30. Abib Pastore, Renan Augusto, 1989-. Diversidade taxonômica e biodegradação de lignina em comunidade bacteriana do solo da caatinga.

Degree: Instituto de Biologia; Programa de Pós-Graduação em Genética e Biologia Molecular, 2016, Universidade Estadual de Campinas

URL: ABIB PASTORE, Renan Augusto. Diversidade taxonômica e biodegradação de lignina em comunidade bacteriana do solo da caatinga. 2016. 1 recurso online (124 p.). Dissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Biologia, Campinas, SP. Disponível em: <http://www.repositorio.unicamp.br/handle/REPOSIP/325040>. Acesso em: 31 ago. 2018. ; http://repositorio.unicamp.br/jspui/handle/REPOSIP/325040

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Orientador: Valéria Maia Merzel

Dissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Biologia

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Orientador: Valéria Maia Merzel

Dissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Biologia

Made available in DSpace on 2018-08-31T03:43:28Z (GMT). No. of bitstreams: 1 Pastore_RenanAugustoAbib_M.pdf: 6525936 bytes, checksum: b495191a0f0e3a719a80b04c1b194c0a (MD5) Previous issue date: 2016

Resumo: A Caatinga é um bioma exclusivamente brasileiro, que cobre aproximadamente 11% do território nacional e considerada a região semiárida mais diversa do mundo. No entanto, o conhecimento acerca da diversidade microbiana presente no solo da Caatinga, bem como do potencial biotecnológico a ela associada, ainda é incipiente e escasso. Atualmente, milhões de toneladas de lignina e compostos relacionados são produzidos como resíduos de efluentes de indústrias de produção de papel e polpa de celulose, com a previsão que este número aumente em um futuro próximo, dado o recente desenvolvimento de combustíveis alternativos gerados da biomassa lignocelulósica. Enzimas microbianas do grupo das ligninases como as lacases têm recebido grande atenção dos cientistas nas últimas décadas, uma vez que a catálise destas enzimas na bioconversão e biodegradação da lignina são exploradas tanto industrialmente (fábricas de papel e celulose, têxtil e produção de biocombustíveis) quanto ambientalmente (desintoxicação de compostos aromáticos recalcitrantes e remediação de solos e rios contaminados). Neste sentido, o presente projeto visou investigar a composição e a dinâmica de comunidades bacterianas nativas do solo da Caatinga sob a influência da adição de lignina ao meio/microcosmo, aplicando uma abordagem polifásica para a análise da diversidade taxonômica. Ao mesmo tempo, bactérias com potencial ligninolítico foram isoladas e exploradas para a prospecção funcional (ABTS, RBBR e Vermelho Congo) e molecular (uso de primers degenerados) de genes de lacases, com vistas à aplicação futura no tratamento de águas residuárias e solos contaminados. Durante 27 dias de amostragem, foram isoladas 156 bactérias potencialmente ligninolíticas (39 oriundas diretamente de microcosmos enriquecidos com 10% de lignina), todas cultivadas em meios específicos com 0,3 % de lignina (m/v). Os dados gerados pelo sequenciamento Illumina do gene RNAr 16S mostrou que a introdução da lignina nos microcosmos proporcionou mudanças na abundância de grupos taxonômicos pertencentes à comunidade bacteriana ao longo do tempo, interferindo na composição da microbiota nos microcosmos avaliados. Enquanto que a maioria dos grupos taxonômicos bacterianos tenha sido suprimida, alguns gêneros como Bacillus e Streptomyces apresentaram significativo aumento de abundância ao longo do enriquecimento, acompanhados também por outros grupos taxonomicamente não descritos, ainda, pela literatura. Os ensaios moleculares e funcionais usados para a prospecção de lacases aparentemente não apresentaram sucesso. No entanto, alguns isolados foram selecionados para ensaios de cromatografia gasosa acoplada à espectrometria de massas (CG-EM), no qual os compostos liberados…

Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS, Oliveira, Valeria Maia de, 1966-, Delforno, Tiago Palladino, Destéfano, Suzete Aparecida Lanza.

Subjects/Keywords: Caatinga; Lignina - Biodegradação; Comunidades bacterianas; RNA ribossômico 16S; Caatinga; Lignin - Biodegradation; Bacterial communities; RNA, Ribosomal, 16S

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APA (6^th Edition):

Abib Pastore, Renan Augusto, 1. (2016). Diversidade taxonômica e biodegradação de lignina em comunidade bacteriana do solo da caatinga. (Masters Thesis). Universidade Estadual de Campinas. Retrieved from ABIB PASTORE, Renan Augusto. Diversidade taxonômica e biodegradação de lignina em comunidade bacteriana do solo da caatinga. 2016. 1 recurso online (124 p.). Dissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Biologia, Campinas, SP. Disponível em: <http://www.repositorio.unicamp.br/handle/REPOSIP/325040>. Acesso em: 31 ago. 2018. ; http://repositorio.unicamp.br/jspui/handle/REPOSIP/325040

Chicago Manual of Style (16^th Edition):

Abib Pastore, Renan Augusto, 1989-. “Diversidade taxonômica e biodegradação de lignina em comunidade bacteriana do solo da caatinga.” 2016. Masters Thesis, Universidade Estadual de Campinas. Accessed April 17, 2021. ABIB PASTORE, Renan Augusto. Diversidade taxonômica e biodegradação de lignina em comunidade bacteriana do solo da caatinga. 2016. 1 recurso online (124 p.). Dissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Biologia, Campinas, SP. Disponível em: <http://www.repositorio.unicamp.br/handle/REPOSIP/325040>. Acesso em: 31 ago. 2018. ; http://repositorio.unicamp.br/jspui/handle/REPOSIP/325040.

MLA Handbook (7^th Edition):

Abib Pastore, Renan Augusto, 1989-. “Diversidade taxonômica e biodegradação de lignina em comunidade bacteriana do solo da caatinga.” 2016. Web. 17 Apr 2021.

Vancouver:

Abib Pastore, Renan Augusto 1. Diversidade taxonômica e biodegradação de lignina em comunidade bacteriana do solo da caatinga. [Internet] [Masters thesis]. Universidade Estadual de Campinas; 2016. [cited 2021 Apr 17]. Available from: ABIB PASTORE, Renan Augusto. Diversidade taxonômica e biodegradação de lignina em comunidade bacteriana do solo da caatinga. 2016. 1 recurso online (124 p.). Dissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Biologia, Campinas, SP. Disponível em: <http://www.repositorio.unicamp.br/handle/REPOSIP/325040>. Acesso em: 31 ago. 2018. ; http://repositorio.unicamp.br/jspui/handle/REPOSIP/325040.

Council of Science Editors:

Abib Pastore, Renan Augusto 1. Diversidade taxonômica e biodegradação de lignina em comunidade bacteriana do solo da caatinga. [Masters Thesis]. Universidade Estadual de Campinas; 2016. Available from: ABIB PASTORE, Renan Augusto. Diversidade taxonômica e biodegradação de lignina em comunidade bacteriana do solo da caatinga. 2016. 1 recurso online (124 p.). Dissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Biologia, Campinas, SP. Disponível em: <http://www.repositorio.unicamp.br/handle/REPOSIP/325040>. Acesso em: 31 ago. 2018. ; http://repositorio.unicamp.br/jspui/handle/REPOSIP/325040

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