subject:(Ribonucleases)

Oregon State University

1. Tiffany, Thomas Oliver. Some physical studies on ribonuclease-S and its component parts, S-protein and S-peptide.

Degree: PhD, Biochemistry and Biophysics, 1969, Oregon State University

URL: http://hdl.handle.net/1957/46117

Subjects/Keywords: Ribonucleases

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APA (6^th Edition):

Tiffany, T. O. (1969). Some physical studies on ribonuclease-S and its component parts, S-protein and S-peptide. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/46117

Chicago Manual of Style (16^th Edition):

Tiffany, Thomas Oliver. “Some physical studies on ribonuclease-S and its component parts, S-protein and S-peptide.” 1969. Doctoral Dissertation, Oregon State University. Accessed January 18, 2021. http://hdl.handle.net/1957/46117.

MLA Handbook (7^th Edition):

Tiffany, Thomas Oliver. “Some physical studies on ribonuclease-S and its component parts, S-protein and S-peptide.” 1969. Web. 18 Jan 2021.

Vancouver:

Tiffany TO. Some physical studies on ribonuclease-S and its component parts, S-protein and S-peptide. [Internet] [Doctoral dissertation]. Oregon State University; 1969. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/1957/46117.

Council of Science Editors:

Tiffany TO. Some physical studies on ribonuclease-S and its component parts, S-protein and S-peptide. [Doctoral Dissertation]. Oregon State University; 1969. Available from: http://hdl.handle.net/1957/46117

Oregon State University

2. Lewis, Roger Allen. The purification and characterization of a ribonuclease from bovine aorta.

Degree: PhD, Biochemistry and Biophysics, 1968, Oregon State University

URL: http://hdl.handle.net/1957/46726

► Reports of investigations on nucleases in arterial tissue are few in number. The present study is concerned with the purification and characterization of an aortic… (more)

Subjects/Keywords: Ribonucleases

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APA (6^th Edition):

Lewis, R. A. (1968). The purification and characterization of a ribonuclease from bovine aorta. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/46726

Chicago Manual of Style (16^th Edition):

Lewis, Roger Allen. “The purification and characterization of a ribonuclease from bovine aorta.” 1968. Doctoral Dissertation, Oregon State University. Accessed January 18, 2021. http://hdl.handle.net/1957/46726.

MLA Handbook (7^th Edition):

Lewis, Roger Allen. “The purification and characterization of a ribonuclease from bovine aorta.” 1968. Web. 18 Jan 2021.

Vancouver:

Lewis RA. The purification and characterization of a ribonuclease from bovine aorta. [Internet] [Doctoral dissertation]. Oregon State University; 1968. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/1957/46726.

Council of Science Editors:

Lewis RA. The purification and characterization of a ribonuclease from bovine aorta. [Doctoral Dissertation]. Oregon State University; 1968. Available from: http://hdl.handle.net/1957/46726

Rutgers University

3. Dissanayake Rallage, Thakshila D., 1981-. A multiscale computational approach to study RNase A catalysis.

Degree: PhD, Computational Biology and Molecular Biophysics, 2016, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/49172/

►

Enzyme catalysis is an extremely important and complex process that is fundamental to biology. Experiments provide a wealth of valuable information about the function of… (more)

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Enzyme catalysis is an extremely important and complex process that is fundamental to biology. Experiments provide a wealth of valuable information about the function of enzymes; however, this information requires the use of computational models to establish a meaningful interpretation that can be used to guide design. Multiscale computational models, which integrate a hierarchy of theoretical methods to address complex biomolecular problems that span large spatial and temporal ranges, afford powerful tools to provide a detailed molecular level interpretation of a wide range of experimental data from which a consensus view of catalytic mechanism may emerge. In this dissertation, I detail my efforts to develop and apply multiscale methods to study the mechanisms of RNA backbone cleavage catalyzed by Ribonuclease A, an important archetype enzyme system, and the factors that regulate its activity. In the first phase of this research, I use molecular dynamics simulations to characterize the structure and dynamics of the active enzyme in solution at different stages along the reaction path. In this work, I demonstrate that the crystallographic structure represents an inactive, catalytically non-relevant state, and make predictions that a conformational change involving the flipping of the side chain of a conserved histidine residue (His12) is required to adopt a catalytically competent conformation. In the second phase of this research, I apply ''constant pH molecular dynamics simulations'' (CpHMD) to characterize the conditional probability of finding key active site residues in a protonation state that supports general acid-base catalysis. This allowed the prediction of pKa shifts for His12, His119 and Lys41, and, for the first time, activity-pH profiles for an enzyme system that can be compared directly with those measured in kinetic experiments. In the third phase of this research, I use combined quantum mechanical/molecular mechanical methods to study the catalytic chemical steps of transphosphorylation. Results of this work predict a free energy landscape for the reaction, from which the minimum free energy pathway that connects reactants and products allows a detailed molecular-level picture of mechanism. In the fourth phase of this research, I extend the CpHMD method to nucleic acid systems, to benchmark the method for the study of ribozymes that catalyze the same reaction as RNase A.

Advisors/Committee Members: York, Darrin M (chair), Case, David A (internal member), Nanda, Vikas (internal member), Jha, Shantenu (outside member).

Subjects/Keywords: Ribonucleases; Catalysts; RNA

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APA (6^th Edition):

Dissanayake Rallage, Thakshila D., 1. (2016). A multiscale computational approach to study RNase A catalysis. (Doctoral Dissertation). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/49172/

Chicago Manual of Style (16^th Edition):

Dissanayake Rallage, Thakshila D., 1981-. “A multiscale computational approach to study RNase A catalysis.” 2016. Doctoral Dissertation, Rutgers University. Accessed January 18, 2021. https://rucore.libraries.rutgers.edu/rutgers-lib/49172/.

MLA Handbook (7^th Edition):

Dissanayake Rallage, Thakshila D., 1981-. “A multiscale computational approach to study RNase A catalysis.” 2016. Web. 18 Jan 2021.

Vancouver:

Dissanayake Rallage, Thakshila D. 1. A multiscale computational approach to study RNase A catalysis. [Internet] [Doctoral dissertation]. Rutgers University; 2016. [cited 2021 Jan 18]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/49172/.

Council of Science Editors:

Dissanayake Rallage, Thakshila D. 1. A multiscale computational approach to study RNase A catalysis. [Doctoral Dissertation]. Rutgers University; 2016. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/49172/

Universidade do Rio Grande do Sul

4. Corrêa, Lauís Brisolara. Diversificação funcional em ribonucleases T2 na família Solanaceae.

Degree: 2015, Universidade do Rio Grande do Sul

URL: http://hdl.handle.net/10183/131927

► As ribonucleases catalisam a clivagem do RNA e são componentes ubíquos das células, desde procariotos até eucariotos. A família T2 é a classe de RNases… (more)

▼ As ribonucleases catalisam a clivagem do RNA e são componentes ubíquos das células, desde procariotos até eucariotos. A família T2 é a classe de RNases mais amplamente distribuída entre os organismos vivos. A ampla distribuição desta família sugere que ela deve ter um papel funcional muito importante na biologia celular destes organismos. Em Solanaceae, há basicamente dois grupos destas proteínas, um representado pelas S-RNases, as quais estão envolvidas com a rejeição do pólen na autoincompatibilidade gametofítica e um outro grupo mais diverso que é denominado de S-like RNases, com funções muito diversificadas. As S-RNases se apresentam na forma de um gene multialélico, altamente polimórfico, que está contido no lócus S. O lócus S é composto por uma combinação de proteínas SLF (S-locus F-box), responsáveis pela determinação do fator polínico, e uma S-RNase produzida apenas no pistilo, de forma que estes genes estão fortemente ligados formando o haplótipo S. Esses produtos gênicos interagem possibilitando a rejeição do auto-pólen num fenômeno denominado distinção colaborativa do pólen não próprio. No Capítulo II, foram utilizados métodos filogenéticos para determinar os principais agrupamentos de alelos na genealogia de S-RNases do gênero Solanum. A topologia da árvore não mostrou sinal filogenético para espécies, contudo, isso era esperado uma vez que a diversificação destes alelos ocorreu anteriormente à diversificação das espécies, gerando um fenômeno denominado de polimorfismos trans-específicos. Além disso, foram realizadas análises de seleção positiva, as quais encontraram um alto número de resíduos com altas probabilidades, indicando que estão sob pressão de seleção positiva darwiniana. Com o intuito de compreender a diversidade estrutural destes alelos, foram construídos modelos teóricos com base em modelagem por homologia dos principais clados da filogenia, já que estas sequências apresentam um elevado grau de polimorfismo. Os resultados mostraram grande variação estrutural na região hipervariável destas sequências, enquanto que regiões conservadas não apresentaram grandes mudanças estruturais e com estruturas secundárias características. No Capítulo III, foram realizadas diversas análises com o objetivo de compreender a diversificação estrutural e funcional de RNases T2 na família Solanaceae. As análises filogenéticas mostraram a formação de três principais grupos, sendo um de S-RNases, e os outros dois de S-like RNases. Com relação ao Clado 2, podemos inferir que houveram ao menos dois eventos de duplicação gênica. Além disso, também foram utilizados os métodos de NSsites e branch-site para inferência de seleção positiva como uma forma de identificar possíveis sinais de diversificação molecular. Muitos resíduos parecem estar sob seleção em ambos os métodos, embora um número maior fosse encontrado no NSsites (41), com estes resíduos localizando-se em regiões mais flexíveis da proteína, enquanto que os aqueles selecionados de acordo com o brach-site (8) estavam situados em posições mais rígidas da… Advisors/Committee Members: Freitas, Loreta Brandao de.

Subjects/Keywords: Solanaceae; Ribonucleases T2; Filogenética

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APA (6^th Edition):

Corrêa, L. B. (2015). Diversificação funcional em ribonucleases T2 na família Solanaceae. (Thesis). Universidade do Rio Grande do Sul. Retrieved from http://hdl.handle.net/10183/131927

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Corrêa, Lauís Brisolara. “Diversificação funcional em ribonucleases T2 na família Solanaceae.” 2015. Thesis, Universidade do Rio Grande do Sul. Accessed January 18, 2021. http://hdl.handle.net/10183/131927.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Corrêa, Lauís Brisolara. “Diversificação funcional em ribonucleases T2 na família Solanaceae.” 2015. Web. 18 Jan 2021.

Vancouver:

Corrêa LB. Diversificação funcional em ribonucleases T2 na família Solanaceae. [Internet] [Thesis]. Universidade do Rio Grande do Sul; 2015. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/10183/131927.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Corrêa LB. Diversificação funcional em ribonucleases T2 na família Solanaceae. [Thesis]. Universidade do Rio Grande do Sul; 2015. Available from: http://hdl.handle.net/10183/131927

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Oregon State University

5. Lynn, Jackson. Preparation and properties of poly-L-tyrosyl acetamidinated ribonuclease.

Degree: PhD, Chemistry, 1966, Oregon State University

URL: http://hdl.handle.net/1957/48202

► Polypeptidyl derivatives of bovine pancreatic ribonuclease (RNase) containing two or three tyrosine chains with an average of two to three residues per chain have been… (more)

▼ Polypeptidyl derivatives of bovine pancreatic ribonuclease (RNase) containing two or three tyrosine chains with an average of two to three residues per chain have been prepared. The effects of various perturbants such as neutral salts, ethanol, urea and guanidine hydrochloride on the thermal transition of the derivatives have been studied. The forces responsible for maintaining the native conformation of RNase appear to be unaffected by chemical modification. In the association-dissociation reaction of the polypeptidyl derivatives of RNase, hydrophobic interactions are strongly implicated. Acetamidinated-RNase I was prepared by treating a RNase solution with excess acetimidate hydrochloride at pH 7.8 in 0.1 N NaHCO₃ buffer containing Na₂SO₄. After removal of reagents by dialysis, polypeptidylation was carried out under the same conditions by reacting the acetamidinated-RNase derivative with excess N-carboxy-L-tyrosine anhydride. There were then obtained a soluble fraction (IA) and an insoluble fraction (IB) of polytyrosyl-acetamidinated RNase (PT-Ac-RNase). IB could be dissolved in 0.0025 N HCl (IC). Fractions IA and IC were purified by gel filtration prior to experimental investigation. Another preparation (PT-Ac-RNase II) was carried out in phosphate buffer under comparable conditions as the preparation of PT-Ac-RNase I. Analysis for added amino acid residues and for the number of sites amidinated or peptidylated indicated that IA contained three peptide chains of average chain length slightly less than three tyrosines. The fraction IC had 17 moles of tyrosine per mole of protein, giving an average chain length of about six tyrosines. PT-Ac-RNase IIA had 4 moles of tyrosine per mole of protein attached to two sites. The enzymatic activities of the PT-Ac-RNase derivatives were shown to be about 5 percent of the unmodified enzyme by two methods of assay. The melting temperatures (Tm) as determined by difference spectra at 287 mμ and 237 mμ for RNase, acetamidinated RNase and PT-Ac-RNase in 0.05 M salt at pH 2.1 were very similar and varied between 33° and 35°. But calculations for Δε₂₈₇ mμ and Δε₂₃₇ revealed respective values of 1000 and 3000 for the PT-Ac-RNase derivatives. The Tm of PT-Ac-RNase IA was shown to be dependent on PH, urea, ethanol and guanidine trydrochloride. Lowering pH or increasing the concentration of perturbant would shift the Tm to lower temperature. However, the same observation has also been reported for native RNase. PT-Ac-RNase IC became insoluble between pH 5 and pH 9, but IA was soluble throughout this pH range. Although turbidity formation was enhanced by increasing concentration of KCI, addition of guanidine hydrochloride could prevent turbidity formation. Increasing concentration of KCI in the PT-Ac-RNase II solution at pH 2.1 shifted the Tm to higher temperatures and could induce turbidity formation at 1.2 M concentration. A similar effect of increasing the Tm of PT-Ac-RNase II was observed with CaCl₂, at pH 2.1. With 0.5 M KSCN… Advisors/Committee Members: Becker, Robert R. (advisor).

Subjects/Keywords: Polypeptides; Ribonucleases

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APA (6^th Edition):

Lynn, J. (1966). Preparation and properties of poly-L-tyrosyl acetamidinated ribonuclease. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/48202

Chicago Manual of Style (16^th Edition):

Lynn, Jackson. “Preparation and properties of poly-L-tyrosyl acetamidinated ribonuclease.” 1966. Doctoral Dissertation, Oregon State University. Accessed January 18, 2021. http://hdl.handle.net/1957/48202.

MLA Handbook (7^th Edition):

Lynn, Jackson. “Preparation and properties of poly-L-tyrosyl acetamidinated ribonuclease.” 1966. Web. 18 Jan 2021.

Vancouver:

Lynn J. Preparation and properties of poly-L-tyrosyl acetamidinated ribonuclease. [Internet] [Doctoral dissertation]. Oregon State University; 1966. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/1957/48202.

Council of Science Editors:

Lynn J. Preparation and properties of poly-L-tyrosyl acetamidinated ribonuclease. [Doctoral Dissertation]. Oregon State University; 1966. Available from: http://hdl.handle.net/1957/48202

Rutgers University

6. Zhu, Qiaoqiao, 1991-. Crosstalk between long non-coding RNAs and circadian chromatin.

Degree: PhD, Circadian rhythms, 2020, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/64311/

►

The circadian rhythm is governed by transcriptional negative feedback facilitated by oscillating histone modifications and chromatin remodeling. The circadian rhythm is entrained by external zeitgebers… (more)

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The circadian rhythm is governed by transcriptional negative feedback facilitated by oscillating histone modifications and chromatin remodeling. The circadian rhythm is entrained by external zeitgebers (light and temperature) and are conserved in Neurospora, Drosophila, zebrafish, and mammals. The clock gene frequency in Neurospora and Period2 in vertebrates have natural antisense transcripts (NATs) whose function is not understood. In this dissertation I examined the connection among the circadian clock, non-coding RNAs and heterochromatin formation on both on the genome-wide and locus-specific level using a multi-organism approach. I performed a genome-wide study, using RNA-seq and ChIP-seq to understand the role of H3 lysine 4 methyltransferase (KMT2/SET-1) and histone H3 lysine 9 methyltransferase (KMT1/DIM-5) in Neurospora to understand the role of 2 seemingly opposing modifications. Integrated analysis of RNA-seq and ChIP-seq showed crosstalk and redistributions between histone H3 lysine 4 tri-methylation (H3K4me3) and histone H3 lysine 9 tri-methylation (H3K9me3). I also examined how perturbing the expression of a diurnal lncRNA affected downstream heterochromatin formation at the telomeres. The core circadian clock controls rhythms in TERRA, a long noncoding RNA that originates from telomeres and my research shows alcohol disrupts the diurnal rhythm in TERRA and heterochromatin at the telomere, which in theory makes telomeres more susceptible to DNA damage. I also examined the Per2 NAT, Per2AS and found the diurnal rhythm in Per2AS is dependent on BMAL1. Using the ChIRP-MS, I identified Per2AS-interacting proteins. Specifically, I found hnRNP M interacts with Per2AS and hnRNP M is required to maintain the normal amplitude and period of Per2. Furthermore, I demonstrate that hnRNP M is necessary for H3K9me3 and H3K27me3 at Per2. These findings support a model where Per2AS may serve as scaffold for hnRNP M and other associated proteins that assist in heterochromatin formation at Per2. Collectively, this dissertation furthers our understanding of the circadian clock, non-coding RNAs, and circadian regulated facultative heterochromatin formation.

Advisors/Committee Members: Belden, William J. (chair), Anthony, Tracy G (internal member), Sarkar, Dipak (internal member), Lee, KiBum (outside member), School of Graduate Studies.

Subjects/Keywords: Ribonucleases; Endocrinology and Animal Biosciences

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APA (6^th Edition):

Zhu, Qiaoqiao, 1. (2020). Crosstalk between long non-coding RNAs and circadian chromatin. (Doctoral Dissertation). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/64311/

Chicago Manual of Style (16^th Edition):

Zhu, Qiaoqiao, 1991-. “Crosstalk between long non-coding RNAs and circadian chromatin.” 2020. Doctoral Dissertation, Rutgers University. Accessed January 18, 2021. https://rucore.libraries.rutgers.edu/rutgers-lib/64311/.

MLA Handbook (7^th Edition):

Zhu, Qiaoqiao, 1991-. “Crosstalk between long non-coding RNAs and circadian chromatin.” 2020. Web. 18 Jan 2021.

Vancouver:

Zhu, Qiaoqiao 1. Crosstalk between long non-coding RNAs and circadian chromatin. [Internet] [Doctoral dissertation]. Rutgers University; 2020. [cited 2021 Jan 18]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/64311/.

Council of Science Editors:

Zhu, Qiaoqiao 1. Crosstalk between long non-coding RNAs and circadian chromatin. [Doctoral Dissertation]. Rutgers University; 2020. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/64311/

Universitat Autònoma de Barcelona

7. Pulido Gómez, David. Expanding the scope of the Ribonuclease A superfamily in the host immune defense system: Structural determinants of human RNases involved in antimicrobial host defense.

Degree: Departament de Bioquímica i Biologia Molecular, 2013, Universitat Autònoma de Barcelona

URL: http://hdl.handle.net/10803/131329

► My PhD project focuses on the structure-function analysis of human antimicrobial RNases, Currently eight functional members have been ascribed to the vertebrate secreted RNase superfamily.… (more)

▼ My PhD project focuses on the structure-function analysis of human antimicrobial RNases, Currently eight functional members have been ascribed to the vertebrate secreted RNase superfamily. Together with their catalytic activity towards RNA substrates, other biological properties have been reported such as the antimicrobial activity of diverse members of the family, therefore suggesting that RNases could have an ancestral host-defence function. The eosinophil cationic protein (ECP) is one of the major components of the secondary granules of human eosinophils. ECP is a highly cationic arginine-rich protein, which displays a high antimicrobial activity against pathogens, such as bacteria, helminths and protozoa. In our laboratory the antimicrobial mechanism of action of several RNases has been extensively studied. In particular, this work enables us to determine: i. Identify the protein pharmacophore by rational structure minimization. ii. Explore the effects that take place at the bacterial cell wall upon protein-interaction. Concretely, highlight the crucial role of the lipopolysaccharide molecule in the mechanism of action of ECP, and their N-terminal peptides. iii. Determine that the amyloid formation at the bacterial surface is crucial for the antimicrobial activity of the protein, and in particular, due to the agglutinating activity. iv. Prove that the sequence and structural determinants of RNases are evolutionary conserved at the N-terminal domain. v. Characterize the sequence and structural determinants required for antimicrobial and agglutinating activities Through RNase rational mutagenesis of the homologous RNases 2 and 3 (EDN and ECP respectively) vi. Discover that either ECP or RNase 7 present antimycobacterial activity trough a similar mechanism as proved against Gram-negative bacteria. In conclusion, the results presented in this thesis work enable us to better understanding of how RNases contribute on the host pathogen clearance and open a new window in order to the design and develop new therapeutic agents as alternative antibiotics. Advisors/Committee Members: [email protected] (authoremail), true (authoremailshow), Boix Borràs, Ester (director), Torrent Burgas, Marc (director), true (authorsendemail).

Subjects/Keywords: Host defense; Antimicrobial; Ribonucleases; Ciències Experimentals; 577

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APA (6^th Edition):

Pulido Gómez, D. (2013). Expanding the scope of the Ribonuclease A superfamily in the host immune defense system: Structural determinants of human RNases involved in antimicrobial host defense. (Thesis). Universitat Autònoma de Barcelona. Retrieved from http://hdl.handle.net/10803/131329

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Pulido Gómez, David. “Expanding the scope of the Ribonuclease A superfamily in the host immune defense system: Structural determinants of human RNases involved in antimicrobial host defense.” 2013. Thesis, Universitat Autònoma de Barcelona. Accessed January 18, 2021. http://hdl.handle.net/10803/131329.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Pulido Gómez, David. “Expanding the scope of the Ribonuclease A superfamily in the host immune defense system: Structural determinants of human RNases involved in antimicrobial host defense.” 2013. Web. 18 Jan 2021.

Vancouver:

Pulido Gómez D. Expanding the scope of the Ribonuclease A superfamily in the host immune defense system: Structural determinants of human RNases involved in antimicrobial host defense. [Internet] [Thesis]. Universitat Autònoma de Barcelona; 2013. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/10803/131329.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Pulido Gómez D. Expanding the scope of the Ribonuclease A superfamily in the host immune defense system: Structural determinants of human RNases involved in antimicrobial host defense. [Thesis]. Universitat Autònoma de Barcelona; 2013. Available from: http://hdl.handle.net/10803/131329

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Michigan State University

8. Howard, Christie Jean. Identification and characterization of ribonucleases in Arabidopsis thaliana.

Degree: PhD, Department of Biochemistry, 1996, Michigan State University

URL: http://etd.lib.msu.edu/islandora/object/etd:24547

Subjects/Keywords: Arabidopsis thaliana; Ribonucleases

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APA (6^th Edition):

Howard, C. J. (1996). Identification and characterization of ribonucleases in Arabidopsis thaliana. (Doctoral Dissertation). Michigan State University. Retrieved from http://etd.lib.msu.edu/islandora/object/etd:24547

Chicago Manual of Style (16^th Edition):

Howard, Christie Jean. “Identification and characterization of ribonucleases in Arabidopsis thaliana.” 1996. Doctoral Dissertation, Michigan State University. Accessed January 18, 2021. http://etd.lib.msu.edu/islandora/object/etd:24547.

MLA Handbook (7^th Edition):

Howard, Christie Jean. “Identification and characterization of ribonucleases in Arabidopsis thaliana.” 1996. Web. 18 Jan 2021.

Vancouver:

Howard CJ. Identification and characterization of ribonucleases in Arabidopsis thaliana. [Internet] [Doctoral dissertation]. Michigan State University; 1996. [cited 2021 Jan 18]. Available from: http://etd.lib.msu.edu/islandora/object/etd:24547.

Council of Science Editors:

Howard CJ. Identification and characterization of ribonucleases in Arabidopsis thaliana. [Doctoral Dissertation]. Michigan State University; 1996. Available from: http://etd.lib.msu.edu/islandora/object/etd:24547

University of Arizona

9. Dale, Bruce Edwin, 1950-. Kinetic and mass transfer characteristics of pancreatic ribonuclease immobilized on porous titania .

Degree: 1976, University of Arizona

URL: http://hdl.handle.net/10150/347893

Subjects/Keywords: Immobilized enzymes.; Ribonucleases.

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APA (6^th Edition):

Dale, Bruce Edwin, 1. (1976). Kinetic and mass transfer characteristics of pancreatic ribonuclease immobilized on porous titania . (Masters Thesis). University of Arizona. Retrieved from http://hdl.handle.net/10150/347893

Chicago Manual of Style (16^th Edition):

Dale, Bruce Edwin, 1950-. “Kinetic and mass transfer characteristics of pancreatic ribonuclease immobilized on porous titania .” 1976. Masters Thesis, University of Arizona. Accessed January 18, 2021. http://hdl.handle.net/10150/347893.

MLA Handbook (7^th Edition):

Dale, Bruce Edwin, 1950-. “Kinetic and mass transfer characteristics of pancreatic ribonuclease immobilized on porous titania .” 1976. Web. 18 Jan 2021.

Vancouver:

Dale, Bruce Edwin 1. Kinetic and mass transfer characteristics of pancreatic ribonuclease immobilized on porous titania . [Internet] [Masters thesis]. University of Arizona; 1976. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/10150/347893.

Council of Science Editors:

Dale, Bruce Edwin 1. Kinetic and mass transfer characteristics of pancreatic ribonuclease immobilized on porous titania . [Masters Thesis]. University of Arizona; 1976. Available from: http://hdl.handle.net/10150/347893

University of Texas – Austin

10. Sidote, David Joseph. Structural characterization of the ribonuclease P protein aRpp29 from the hyperthermophilic sulfate-reducing Archaeon Archaeoglobus fulgidus.

Degree: PhD, Molecular Biology, 2004, University of Texas – Austin

URL: http://hdl.handle.net/2152/1409

Subjects/Keywords: Ribonucleases; Proteins – Analysis

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APA (6^th Edition):

Sidote, D. J. (2004). Structural characterization of the ribonuclease P protein aRpp29 from the hyperthermophilic sulfate-reducing Archaeon Archaeoglobus fulgidus. (Doctoral Dissertation). University of Texas – Austin. Retrieved from http://hdl.handle.net/2152/1409

Chicago Manual of Style (16^th Edition):

Sidote, David Joseph. “Structural characterization of the ribonuclease P protein aRpp29 from the hyperthermophilic sulfate-reducing Archaeon Archaeoglobus fulgidus.” 2004. Doctoral Dissertation, University of Texas – Austin. Accessed January 18, 2021. http://hdl.handle.net/2152/1409.

MLA Handbook (7^th Edition):

Sidote, David Joseph. “Structural characterization of the ribonuclease P protein aRpp29 from the hyperthermophilic sulfate-reducing Archaeon Archaeoglobus fulgidus.” 2004. Web. 18 Jan 2021.

Vancouver:

Sidote DJ. Structural characterization of the ribonuclease P protein aRpp29 from the hyperthermophilic sulfate-reducing Archaeon Archaeoglobus fulgidus. [Internet] [Doctoral dissertation]. University of Texas – Austin; 2004. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/2152/1409.

Council of Science Editors:

Sidote DJ. Structural characterization of the ribonuclease P protein aRpp29 from the hyperthermophilic sulfate-reducing Archaeon Archaeoglobus fulgidus. [Doctoral Dissertation]. University of Texas – Austin; 2004. Available from: http://hdl.handle.net/2152/1409

University of Tennessee – Knoxville

11. Bafna, Khushboo. Conformational Sub-states and Dynamics in Human Ribonuclease Family.

Degree: 2019, University of Tennessee – Knoxville

URL: https://trace.tennessee.edu/utk_graddiss/5683

► The enzymes of the ribonuclease (RNase) family catalyze the hydrolysis of ribonucleic acid (RNA). There is a wide interest in therapeutic interventions of human RNases… (more)

▼ The enzymes of the ribonuclease (RNase) family catalyze the hydrolysis of ribonucleic acid (RNA). There is a wide interest in therapeutic interventions of human RNases (hRNases) due to their critical role in host defense, cancer cell proliferation and neurodegenerative diseases. Development of structure-based therapeutics targeting individual members of a closely related enzyme family is difficult. The structural conservation among hRNases cannot explain a million-fold difference in the catalytic efficiency of these enzymes. We hypothesize, this ambiguity in the structure activity relationship of hRNases can be explained by their dynamical behavior. The rate of substrate turnover in RNases correlates strongly with the rates of conformational dynamics. Moreover, catalytic efficiency is also linked to the ability of an enzyme to sample conformational sub-states that promote specific interaction between reactants at various stages of catalysis. Detailed characterization of the structure-function-dynamics relationship in hRNases can help in determining similarities and differences in the motions associated to catalysis thereby enhancing the possible druggability of this enzyme family. This study investigates the role of functionally relevant conformational sub-states and dynamics in each step of the RNase members catalytic cycle; apo, substrate-bound, the chemical step, and product release. Computer simulations and nuclear magnetic resonance (NMR) relaxation dispersion experiments indicated an increased dynamics in distal loop regions of RNases in their apo state. Similar dynamical patterns were observed within the members of an individual sub-family, while dynamics were different across sub-families. Nucleotide binding properties probed using computer simulations indicated diverse binding preferences in hRNases. Additionally, structural and dynamical properties of hRNases varied significantly with subtle change in temperature. Quasi anharmonic analysis revealed sampling of separate conformational sub-states in product release step of RNases. Further, NMR chemical shift titrations along with the chemical shift projection analysis revealed distinct conformational rearrangements in hRNases upon binding of ligands that mimic cleaved products. Steady-state kinetics experiments showed million-fold difference in the catalytic efficiency of hRNases. Finally, hybrid quantum mechanical/molecular mechanics calculations identified conformational sub-states associated with the chemical step in bovine RNase A. Broadly, these results strongly support our hypothesis that dynamics modulates catalytic efficiencies of structurally related enzyme super-families like RNases.

Subjects/Keywords: Human ribonucleases; protein dynamics; conformational sub-states; catalytic cycle

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bafna, K. (2019). Conformational Sub-states and Dynamics in Human Ribonuclease Family. (Doctoral Dissertation). University of Tennessee – Knoxville. Retrieved from https://trace.tennessee.edu/utk_graddiss/5683

Chicago Manual of Style (16^th Edition):

Bafna, Khushboo. “Conformational Sub-states and Dynamics in Human Ribonuclease Family.” 2019. Doctoral Dissertation, University of Tennessee – Knoxville. Accessed January 18, 2021. https://trace.tennessee.edu/utk_graddiss/5683.

MLA Handbook (7^th Edition):

Bafna, Khushboo. “Conformational Sub-states and Dynamics in Human Ribonuclease Family.” 2019. Web. 18 Jan 2021.

Vancouver:

Bafna K. Conformational Sub-states and Dynamics in Human Ribonuclease Family. [Internet] [Doctoral dissertation]. University of Tennessee – Knoxville; 2019. [cited 2021 Jan 18]. Available from: https://trace.tennessee.edu/utk_graddiss/5683.

Council of Science Editors:

Bafna K. Conformational Sub-states and Dynamics in Human Ribonuclease Family. [Doctoral Dissertation]. University of Tennessee – Knoxville; 2019. Available from: https://trace.tennessee.edu/utk_graddiss/5683

Tartu University

12. Paier, Anton. Ribosome Degradation in Living Bacteria .

Degree: 2017, Tartu University

URL: http://hdl.handle.net/10062/56325

► Ribosoomid on makromolekulaarsed kompleksid, mis koosnevad kahest suurest ja ühest väikesest RNAst ja paljudest erinevatest valkudest. Ribosoomides sünteesitakse kõik valgud, mida organismis leida võib, ning… (more)

▼ Ribosoomid on makromolekulaarsed kompleksid, mis koosnevad kahest suurest ja ühest väikesest RNAst ja paljudest erinevatest valkudest. Ribosoomides sünteesitakse kõik valgud, mida organismis leida võib, ning aktiivsete ribsoomide konsentratsioon (ja seega sünteesi kiirus) limiteerib rakkude kasvu kiirust. Ehk teisisõnu, mida kiiremini sünteesitakse uusi ribosoome, seda kiiremini kasvab ja jaguneb ka rakk. Kuna ribosomaalse RNA süntees hõlmab ca 80% raku RNA sünteesi aktiivsusest ja ribosoomi valgud moodustavad kuni veerandi raku valgumassist on selge, et mitte ainult ribosoomide funktsioon valgusünteesil vaid ka nende metabolism on rakulises majapidamises määrava tähtsusega. Tõepoolest, juba mõnda aega on teada, et aeglaselt kasvavates bakterirakkudes tegeleb enamus raku RNA lagundamise võimekusest värskelt sünteesitud ribosomaalse RNA lagundamisega. Sellegipoolest on viimase 50 aasta vältel üldiselt usutud, et kord juba valmis tehtud ja kokku pakitud ribosoomid on äärmiselt stabiilsed ning, et neid lagundatakse vaid tugeva stressi tingimustes. Samuti on meie teadmised ribosoomide lagundamise molekulaarsetest mehhanismidest bakteris üsnagi piiratud. Käesoleva doktoritöö eesmärk on kirjeldada ribosoomide lagundamist kasvavates soolekepikese (Escherichia coli) rakkudes ja heita valgust ribsoomide lagundamise mehhanismidele, molekulaarsetele radadele ning ensüümidele, mis selles protsessis osalevad. Me avastasime üllatusega, et kuigi ribosoome tõepoolest lagundatakse kasvavates bakterirakkudes, toimub see protsess vaid rakukultuuri kasvu aeglustumise perioodil, mis eelneb statsionaarse kasvufaasi saabumisele. Meil ei õnnestunud tuvastada küpsete ribosoomide lagundamist ei ühtlase kiirusega kasvavates ega ka null-kiirusega kasvavates rakkudes. Võimalik, et ribosoomide lagundamine aitab rakke neid ette valmistades eluks statsionaarses faasis, mil ei vajata suurt valgusünteesi võimekust, küll aga vabu komponente, millest elutingimuste paranedes kiiresti uusi makromolekule tootma hakata. Lisaks leidsime, et osad (kuid mitte kõik) ribosoomi RNAd inaktiveerivad mutatsioonid viivad samuti ribsoomide lagundamisele, kuid miskipärast lagundatakse siis nii mutantseid ning inaktiivseid kui metsiktüüpi ning aktiivseid ribosoome. Jällegi viitab see, et ribsoomide lagundamise eesmärk võiks olla üldise ribosoomide konsentratsiooni alandamine rakus. Kui me lisasime ribsoomide lagundamise katsesüsteemi valgusünteesi pärssivat antibiootikumi kloramfenikool, päästsime me sellega ribosoomid lagundamisest. Seda tulemust võib tõlgendada viisil, et de novo valgusüntees on vajalik ribosoomide lagundamisprogrammi käivitamiseks rakus. Testides ribosoomide lagundamise võime osas bakteritüvesid, kus puuduvad erinevad RNAd lagundavad ensüümid, leidsime kaks ensüümi, mille puudumise korral ribosoome ei lagundatud. Neist esimene, RNaas R, lõhub RNAsid alates nende tagumisest ehk 3’ otsast ning tunneb erilist lõbu kõrge sekundaarstruktuuriga RNA-de hävitamisest. RNaas R on ka eelnevalt näidatud osalevat ribosoomide lagundamisel. Teine ensüüm on… Advisors/Committee Members: Maiväli, Ülo, juhendaja (advisor), Tenson, Tanel, juhendaja (advisor).

Subjects/Keywords: ribosoomid; lagundamine; bakterid; Escherichia coli; ribonukleaasid; ribosomes; decomposition; bacteria; ribonucleases

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Paier, A. (2017). Ribosome Degradation in Living Bacteria . (Thesis). Tartu University. Retrieved from http://hdl.handle.net/10062/56325

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Paier, Anton. “Ribosome Degradation in Living Bacteria .” 2017. Thesis, Tartu University. Accessed January 18, 2021. http://hdl.handle.net/10062/56325.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Paier, Anton. “Ribosome Degradation in Living Bacteria .” 2017. Web. 18 Jan 2021.

Vancouver:

Paier A. Ribosome Degradation in Living Bacteria . [Internet] [Thesis]. Tartu University; 2017. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/10062/56325.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Paier A. Ribosome Degradation in Living Bacteria . [Thesis]. Tartu University; 2017. Available from: http://hdl.handle.net/10062/56325

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

13. ΜΑΟΥΡΗ, ΑΝΑΣΤΑΣΙΑ. Η ΡΙΒΟΝΟΥΚΛΕΟΛΥΤΙΚΗ ΔΡΑΣΗ ΤΟΥ ΚΥΤΤΑΡΟΠΛΑΣΜΑΤΟΣ ΤΟΥ ΠΡΩΤΟΖΩΟΥ TETRAHYMENA PYRIFORMIS.

Degree: 1987, Αριστοτέλειο Πανεπιστήμιο Θεσσαλονίκης (ΑΠΘ); Aristotle University Of Thessaloniki (AUTH)

URL: http://hdl.handle.net/10442/hedi/0187

►

ΑΠΟ ΤΟ ΚΥΤΤΑΡΟΠΛΑΣΜΑ ΤΟΥ ΠΡΩΤΟΖΩΟΥ TETRAHYMENA PYRIFORMIS ΑΠΟΜΟΝΩΘΗΚΑΝ ΔΥΟ ΟΞΙΝΕΣ (Α, Β) ΚΑΙ ΜΙΑ ΟΥΔΕΤΕΡΗ (Γ) ΡΙΒΟΝΟΥΚΛΕΑΣΕΣ. ΜΕΛΕΤΗΘΗΚΕ Η ΕΠΙΔΡΑΣΗ ΤΟΥ ΡΗ, ΤΩΝ ΜΟΝΟΣΘΕΝΩΝ ΙΟΝΤΩΝ,… (more)

Subjects/Keywords: ISOLATION OF RIBONUCLEASES; PROPERTIES OF RIBONUCLEASES; Ribonucleases; RIBONUCLEASES IN TETRAHYMENA PYRIFORMIS; SPECIFICITY OF RIBONUCLEASES; ΑΠΟΜΟΝΩΣΗ ΡΙΒΟΝΟΥΚΛΕΑΣΩΝ; Βιοχημεία; ΕΞΕΙΔΙΚΕΥΣΗ ΡΙΒΟΝΟΥΚΛΕΑΣΩΝ; ΙΔΙΟΤΗΤΕΣ ΡΙΒΟΝΟΥΚΛΕΑΣΩΝ; Ριβονουκλεάσες; ΡΙΒΟΝΟΥΚΛΕΑΣΕΣ ΤΗΣ TETRAHYMENA RYRIFORMIS

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

ΜΑΟΥΡΗ, �. (1987). Η ΡΙΒΟΝΟΥΚΛΕΟΛΥΤΙΚΗ ΔΡΑΣΗ ΤΟΥ ΚΥΤΤΑΡΟΠΛΑΣΜΑΤΟΣ ΤΟΥ ΠΡΩΤΟΖΩΟΥ TETRAHYMENA PYRIFORMIS. (Thesis). Αριστοτέλειο Πανεπιστήμιο Θεσσαλονίκης (ΑΠΘ); Aristotle University Of Thessaloniki (AUTH). Retrieved from http://hdl.handle.net/10442/hedi/0187

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

ΜΑΟΥΡΗ, ΑΝΑΣΤΑΣΙΑ. “Η ΡΙΒΟΝΟΥΚΛΕΟΛΥΤΙΚΗ ΔΡΑΣΗ ΤΟΥ ΚΥΤΤΑΡΟΠΛΑΣΜΑΤΟΣ ΤΟΥ ΠΡΩΤΟΖΩΟΥ TETRAHYMENA PYRIFORMIS.” 1987. Thesis, Αριστοτέλειο Πανεπιστήμιο Θεσσαλονίκης (ΑΠΘ); Aristotle University Of Thessaloniki (AUTH). Accessed January 18, 2021. http://hdl.handle.net/10442/hedi/0187.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

ΜΑΟΥΡΗ, ΑΝΑΣΤΑΣΙΑ. “Η ΡΙΒΟΝΟΥΚΛΕΟΛΥΤΙΚΗ ΔΡΑΣΗ ΤΟΥ ΚΥΤΤΑΡΟΠΛΑΣΜΑΤΟΣ ΤΟΥ ΠΡΩΤΟΖΩΟΥ TETRAHYMENA PYRIFORMIS.” 1987. Web. 18 Jan 2021.

Vancouver:

ΜΑΟΥΡΗ �. Η ΡΙΒΟΝΟΥΚΛΕΟΛΥΤΙΚΗ ΔΡΑΣΗ ΤΟΥ ΚΥΤΤΑΡΟΠΛΑΣΜΑΤΟΣ ΤΟΥ ΠΡΩΤΟΖΩΟΥ TETRAHYMENA PYRIFORMIS. [Internet] [Thesis]. Αριστοτέλειο Πανεπιστήμιο Θεσσαλονίκης (ΑΠΘ); Aristotle University Of Thessaloniki (AUTH); 1987. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/10442/hedi/0187.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

ΜΑΟΥΡΗ �. Η ΡΙΒΟΝΟΥΚΛΕΟΛΥΤΙΚΗ ΔΡΑΣΗ ΤΟΥ ΚΥΤΤΑΡΟΠΛΑΣΜΑΤΟΣ ΤΟΥ ΠΡΩΤΟΖΩΟΥ TETRAHYMENA PYRIFORMIS. [Thesis]. Αριστοτέλειο Πανεπιστήμιο Θεσσαλονίκης (ΑΠΘ); Aristotle University Of Thessaloniki (AUTH); 1987. Available from: http://hdl.handle.net/10442/hedi/0187

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universitat de Girona

14. Torrent Albertí, Gerard. L´onconasa com a model per l´estudi de les bases moleculars de la citotoxicitat de les ribonucleases pancreàtiques. Aplicacions a la construcció de ribonucleases amb activitat antimicrobiana.

Degree: Departament de Biologia, 2009, Universitat de Girona

URL: http://hdl.handle.net/10803/7636

► The first part of this work has been focused on the characterization and optimization of the activation process of the recombinant onconase to get an… (more)

Subjects/Keywords: Estabilitat proteica; Citotoxicitat; Activitat bactericida; Inhibidor proteic de ribonucleases; Ribonucleases pancreàtiques; Onconasa; 577

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Torrent Albertí, G. (2009). L´onconasa com a model per l´estudi de les bases moleculars de la citotoxicitat de les ribonucleases pancreàtiques. Aplicacions a la construcció de ribonucleases amb activitat antimicrobiana. (Thesis). Universitat de Girona. Retrieved from http://hdl.handle.net/10803/7636

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Torrent Albertí, Gerard. “L´onconasa com a model per l´estudi de les bases moleculars de la citotoxicitat de les ribonucleases pancreàtiques. Aplicacions a la construcció de ribonucleases amb activitat antimicrobiana.” 2009. Thesis, Universitat de Girona. Accessed January 18, 2021. http://hdl.handle.net/10803/7636.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Torrent Albertí, Gerard. “L´onconasa com a model per l´estudi de les bases moleculars de la citotoxicitat de les ribonucleases pancreàtiques. Aplicacions a la construcció de ribonucleases amb activitat antimicrobiana.” 2009. Web. 18 Jan 2021.

Vancouver:

Torrent Albertí G. L´onconasa com a model per l´estudi de les bases moleculars de la citotoxicitat de les ribonucleases pancreàtiques. Aplicacions a la construcció de ribonucleases amb activitat antimicrobiana. [Internet] [Thesis]. Universitat de Girona; 2009. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/10803/7636.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Torrent Albertí G. L´onconasa com a model per l´estudi de les bases moleculars de la citotoxicitat de les ribonucleases pancreàtiques. Aplicacions a la construcció de ribonucleases amb activitat antimicrobiana. [Thesis]. Universitat de Girona; 2009. Available from: http://hdl.handle.net/10803/7636

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Rhodes University

15. Sennuga, Afolake Temitope. Biological synthesis of metallic nanoparticles and their interactions with various biomedical targets.

Degree: Faculty of Science, Biochemistry, Microbiology and Biotechnology, 2012, Rhodes University

URL: http://hdl.handle.net/10962/d1004069

► The synthesis of nanostructured materials, especially metallic nanoparticles, has accrued utmost interest over the past decade owing to their unique properties that make them applicable… (more)

▼ The synthesis of nanostructured materials, especially metallic nanoparticles, has accrued utmost interest over the past decade owing to their unique properties that make them applicable in different fields of science and technology. The limitation to the use of these nanoparticles is the paucity of an effective method of synthesis that will produce homogeneous size and shape nanoparticles as well as particles with limited or no toxicity to the human health and the environment. The biological method of nanoparticle synthesis is a relatively simple, cheap and environmentally friendly method than the conventional chemical method of synthesis and thus gains an upper hand. The biomineralization of nanoparticles in protein cages is one of such biological approaches used in the generation of nanoparticles. This method of synthesis apart from being a safer method in the production of nanoparticles is also able to control particle morphology. In this study, a comparative biological synthesis, characterization and biomedical effects of metallic nanoparticles of platinum, gold and silver were investigated. Metallic nanoparticles were biologically synthesized using cage-like (apoferritin), barrel-like (GroEL) and non-caged (ribonuclease) proteins. Nanoparticles generated were characterized using common techniques such as UV-visible spectroscopy, scanning and transmission electron microscopy, inductively coupled optical emission spectroscopy, Fourier transform infra-red spectroscopy and energy dispersion analysis of X-rays (EDAX). Nanoparticles synthesised biologically using apoferritin, GroEL and RNase with exhibited similar chemical and physical properties as thoses nanoparticles generated chemically. In addition, the metallic nanoparticles fabricated within the cage-like and barrel-like cavities of apoferritin and GroEL respectively, resulted in nanoparticles with relatively uniform morphology as opposed to those obtained with the non-caged ribonuclease. The enzymatic (ferroxidase) activity of apoferritin was found to be greatly enhanced with platinum (9-fold), gold (7-fold) and silver (54-fold) nanoparticles. The ATPase activity of GroEL was inhibited by silver nanoparticles (64%), was moderately activated by gold nanoparticles (47%) and considerably enhanced by platinum nanoparticles (85%). The hydrolytic activity of RNase was however, lowered by these metallic nanoparticles (90% in Ag nanoparticles) and to a higher degree with platinum (95%) and gold nanoparticles (~100%). The effect of synthesized nanoparticles on the respective enzyme activities of these proteins was also investigated and the potential neurotoxic property of these particles was also determined by an in vitro interaction with acetylcholinesterase. Protein encapsulated nanoparticles with apoferrtin and GroEL showed a decreased inhibition of acetylcholinesterase (<50%) compared with nanoparticles attached to ribonuclease (>50%). Thus, it can be concluded that the cavities of apoferitin and GroEL acted as nanobiofactories for the synthesis and confinement of…

Subjects/Keywords: Nanoparticles; Biosynthesis; Nanotechnology; Biomineralization; Morphology; Ceruloplasmin; Ribonucleases; Adenosine triphosphatase; Acetylcholinesterase; Platinum; Gold; Silver

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sennuga, A. T. (2012). Biological synthesis of metallic nanoparticles and their interactions with various biomedical targets. (Thesis). Rhodes University. Retrieved from http://hdl.handle.net/10962/d1004069

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Sennuga, Afolake Temitope. “Biological synthesis of metallic nanoparticles and their interactions with various biomedical targets.” 2012. Thesis, Rhodes University. Accessed January 18, 2021. http://hdl.handle.net/10962/d1004069.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Sennuga, Afolake Temitope. “Biological synthesis of metallic nanoparticles and their interactions with various biomedical targets.” 2012. Web. 18 Jan 2021.

Vancouver:

Sennuga AT. Biological synthesis of metallic nanoparticles and their interactions with various biomedical targets. [Internet] [Thesis]. Rhodes University; 2012. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/10962/d1004069.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Sennuga AT. Biological synthesis of metallic nanoparticles and their interactions with various biomedical targets. [Thesis]. Rhodes University; 2012. Available from: http://hdl.handle.net/10962/d1004069

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Nova

16. Silva, Cátia Cláudia Bárria da. The role of ribonuclease R in bacterial adaptation to cold shock.

Degree: 2011, Universidade Nova

URL: http://www.rcaap.pt/detail.jsp?id=oai:run.unl.pt:10362/6634

► RESUMO:Os microrganismos reagem à súbita descida de temperatura através de uma resposta adaptativa específica que assegura a sua sobrevivência em condições desfavoráveis. Esta adaptação inclui… (more)

▼ RESUMO:Os microrganismos reagem à súbita descida de temperatura através de uma resposta adaptativa específica que assegura a sua sobrevivência em condições desfavoráveis. Esta adaptação inclui alterações na composição da membrana, na maquinaria de tradução e transcrição. A resposta ao choque térmico pelo frio induz uma repressão da transcrição. No entanto, a descida de temperatura induz a produção de um grupo de proteínas específicas que ajudam a ajustar/re-ajustar o metabolismo celular às novas condições ambientais. Em E. coli o processo de adaptação demora apenas quatro horas, no qual um grupo de proteínas específicas são induzidas. Depois desde período recomeça lentamente a produção de proteínas.A ribonuclease R, uma das proteínas induzidas durante o choque térmico pelo frio, é uma das principais ribonucleases em E. coli envolvidas na degradação do RNA. É uma exoribonuclease que degrada RNA de cadeia dupla, possui funções importantes na maturação e “turnover” do RNA, libertação de ribossomas e controlo de qualidade de proteínas e RNAs. O nível celular desta enzima aumenta até dez vezes após exposição ao frio e estabiliza em células na fase estacionária. A capacidade de degradar RNA de dupla cadeia é importante a baixas temperaturas quando as estruturas de RNA estão mais estáveis. No entanto, este mecanismo é desconhecido. Embora a resposta específica ao “cold shock” tenha sido descoberta há mais de duas décadas e o número de proteínas envolvidas sugerirem que esta adaptação é rápida e simples, continuamos longe de compreender este processo. No nosso trabalho pretendemos descobrir proteínas que interactuem com a RNase R em condições ambientais diferentes através do método “TAP-tag” e espectrometria de massa. A informação obtida pode ser utilizada para deduzir algumas das novas funções da RNase R durante a adaptação bacteriana ao frio e durante a fase estacionária. Mais importante ainda, RNase R poderá ser recrutada para um complexo de proteínas de elevado peso molecular durante o “cold-shock”. – – – – ABSTRACT:Microorganisms react to the rapid temperature downshift with a specific adaptative response that ensures their survival in unfavorable conditions. Adaptation includes changes in membrane composition, in translation and transcription machinery. Cold shock response leads to overall repression of translation. However, temperature downshift induces production of a set of specific proteins that help to tune cell metabolism and readjust it to the new environmental conditions. For Escherichia coli the adaptation process takes only about four hours with a relatively small set of specifically induced proteins involved. After this time, protein production resumes, although at a slower rate. One of the cold inducible proteins is RNase R, one of the main E. coli ribonucleases involved in RNA degradation. RNase R is an exoribonuclease that digest double stranded RNA, serves important functions in RNA maturation and turnover, release of stalled ribosomes by trans-translation, and RNA and protein quality control. The… Advisors/Committee Members: Arraiano, Cecília Maria.

Subjects/Keywords: Ribonucleases - metabolism; Escherichia coli - metabolism; RNA Ribosomes; Cold Temperature; Microbiologia e Parasitologia

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Silva, C. C. B. d. (2011). The role of ribonuclease R in bacterial adaptation to cold shock. (Thesis). Universidade Nova. Retrieved from http://www.rcaap.pt/detail.jsp?id=oai:run.unl.pt:10362/6634

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Silva, Cátia Cláudia Bárria da. “The role of ribonuclease R in bacterial adaptation to cold shock.” 2011. Thesis, Universidade Nova. Accessed January 18, 2021. http://www.rcaap.pt/detail.jsp?id=oai:run.unl.pt:10362/6634.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Silva, Cátia Cláudia Bárria da. “The role of ribonuclease R in bacterial adaptation to cold shock.” 2011. Web. 18 Jan 2021.

Vancouver:

Silva CCBd. The role of ribonuclease R in bacterial adaptation to cold shock. [Internet] [Thesis]. Universidade Nova; 2011. [cited 2021 Jan 18]. Available from: http://www.rcaap.pt/detail.jsp?id=oai:run.unl.pt:10362/6634.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Silva CCBd. The role of ribonuclease R in bacterial adaptation to cold shock. [Thesis]. Universidade Nova; 2011. Available from: http://www.rcaap.pt/detail.jsp?id=oai:run.unl.pt:10362/6634

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Michigan State University

17. Bariola, Pauline Anne. The RNS family of s-like ribonucleases of Arabidopsis thaliana : structures, expression and functions.

Degree: PhD, Department of Biochemistry, 1996, Michigan State University

URL: http://etd.lib.msu.edu/islandora/object/etd:24565

Subjects/Keywords: Arabidopsis thaliana; Ribonucleases – Structure-activity relationships

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APA (6^th Edition):

Bariola, P. A. (1996). The RNS family of s-like ribonucleases of Arabidopsis thaliana : structures, expression and functions. (Doctoral Dissertation). Michigan State University. Retrieved from http://etd.lib.msu.edu/islandora/object/etd:24565

Chicago Manual of Style (16^th Edition):

Bariola, Pauline Anne. “The RNS family of s-like ribonucleases of Arabidopsis thaliana : structures, expression and functions.” 1996. Doctoral Dissertation, Michigan State University. Accessed January 18, 2021. http://etd.lib.msu.edu/islandora/object/etd:24565.

MLA Handbook (7^th Edition):

Bariola, Pauline Anne. “The RNS family of s-like ribonucleases of Arabidopsis thaliana : structures, expression and functions.” 1996. Web. 18 Jan 2021.

Vancouver:

Bariola PA. The RNS family of s-like ribonucleases of Arabidopsis thaliana : structures, expression and functions. [Internet] [Doctoral dissertation]. Michigan State University; 1996. [cited 2021 Jan 18]. Available from: http://etd.lib.msu.edu/islandora/object/etd:24565.

Council of Science Editors:

Bariola PA. The RNS family of s-like ribonucleases of Arabidopsis thaliana : structures, expression and functions. [Doctoral Dissertation]. Michigan State University; 1996. Available from: http://etd.lib.msu.edu/islandora/object/etd:24565

Uniwersytet im. Adama Mickiewicza w Poznaniu

18. Kozłowski, Michał. Synteza i charakterystyka kompleksów lantanowców i ich zastosowanie jako syntetyczne rybonukleazy .

Degree: 2012, Uniwersytet im. Adama Mickiewicza w Poznaniu

URL: http://hdl.handle.net/10593/2662

► Związki kompleksowe lantanowców typu zasad Schiffa od wielu lat cieszą się dużym zainteresowaniem, ze względu na możliwość wykorzystania ich w wielu dziedzinach nauki, takich jak… (more)

Subjects/Keywords: lantanowce; lanthanides; zasady Schiffa; Schiff base; związki kompleksowe; complexes; syntetyczne rybonukleazy; synthetic ribonucleases

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APA (6^th Edition):

Kozłowski, M. (2012). Synteza i charakterystyka kompleksów lantanowców i ich zastosowanie jako syntetyczne rybonukleazy . (Doctoral Dissertation). Uniwersytet im. Adama Mickiewicza w Poznaniu. Retrieved from http://hdl.handle.net/10593/2662

Chicago Manual of Style (16^th Edition):

Kozłowski, Michał. “Synteza i charakterystyka kompleksów lantanowców i ich zastosowanie jako syntetyczne rybonukleazy .” 2012. Doctoral Dissertation, Uniwersytet im. Adama Mickiewicza w Poznaniu. Accessed January 18, 2021. http://hdl.handle.net/10593/2662.

MLA Handbook (7^th Edition):

Kozłowski, Michał. “Synteza i charakterystyka kompleksów lantanowców i ich zastosowanie jako syntetyczne rybonukleazy .” 2012. Web. 18 Jan 2021.

Vancouver:

Kozłowski M. Synteza i charakterystyka kompleksów lantanowców i ich zastosowanie jako syntetyczne rybonukleazy . [Internet] [Doctoral dissertation]. Uniwersytet im. Adama Mickiewicza w Poznaniu; 2012. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/10593/2662.

Council of Science Editors:

Kozłowski M. Synteza i charakterystyka kompleksów lantanowców i ich zastosowanie jako syntetyczne rybonukleazy . [Doctoral Dissertation]. Uniwersytet im. Adama Mickiewicza w Poznaniu; 2012. Available from: http://hdl.handle.net/10593/2662

Michigan State University

19. LeBrasseur, Nicole D. Regulation and function of Arabidopsis thaliana secreted ribonucleases.

Degree: PhD, Genetics Program, 2001, Michigan State University

URL: http://etd.lib.msu.edu/islandora/object/etd:31067

Subjects/Keywords: Arabidopsis thaliana – Genetics; Ribonucleases – Secretion – Regulations; Genetic regulation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

LeBrasseur, N. D. (2001). Regulation and function of Arabidopsis thaliana secreted ribonucleases. (Doctoral Dissertation). Michigan State University. Retrieved from http://etd.lib.msu.edu/islandora/object/etd:31067

Chicago Manual of Style (16^th Edition):

LeBrasseur, Nicole D. “Regulation and function of Arabidopsis thaliana secreted ribonucleases.” 2001. Doctoral Dissertation, Michigan State University. Accessed January 18, 2021. http://etd.lib.msu.edu/islandora/object/etd:31067.

MLA Handbook (7^th Edition):

LeBrasseur, Nicole D. “Regulation and function of Arabidopsis thaliana secreted ribonucleases.” 2001. Web. 18 Jan 2021.

Vancouver:

LeBrasseur ND. Regulation and function of Arabidopsis thaliana secreted ribonucleases. [Internet] [Doctoral dissertation]. Michigan State University; 2001. [cited 2021 Jan 18]. Available from: http://etd.lib.msu.edu/islandora/object/etd:31067.

Council of Science Editors:

LeBrasseur ND. Regulation and function of Arabidopsis thaliana secreted ribonucleases. [Doctoral Dissertation]. Michigan State University; 2001. Available from: http://etd.lib.msu.edu/islandora/object/etd:31067

University of Helsinki

20. Grönroos, Silvia. Noroviruksen desinfektio : geenimonistusmenetelmän luotettavuuden parantaminen.

Degree: Institute of Clinical Medicine; Helsingfors universitet, Medicinska fakulteten, Institutionen för klinisk medicin, 2010, University of Helsinki

URL: http://hdl.handle.net/10138/16231

► Tässä työssä käytettiin pohjana aikaisemmin julkaistua työtä (Nuanualsuwan ja Cliver, 2002), ja tarkoituksena oli tutkia työssä kuvatun menetelmän soveltuvuutta noroviruksen havaitsemiseen sekä sitä, kuinka erilaiset… (more)

Subjects/Keywords: norovirus; disinfection; RT-PCR; ribonucleases; endopeptidase K

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Grönroos, S. (2010). Noroviruksen desinfektio : geenimonistusmenetelmän luotettavuuden parantaminen. (Masters Thesis). University of Helsinki. Retrieved from http://hdl.handle.net/10138/16231

Chicago Manual of Style (16^th Edition):

Grönroos, Silvia. “Noroviruksen desinfektio : geenimonistusmenetelmän luotettavuuden parantaminen.” 2010. Masters Thesis, University of Helsinki. Accessed January 18, 2021. http://hdl.handle.net/10138/16231.

MLA Handbook (7^th Edition):

Grönroos, Silvia. “Noroviruksen desinfektio : geenimonistusmenetelmän luotettavuuden parantaminen.” 2010. Web. 18 Jan 2021.

Vancouver:

Grönroos S. Noroviruksen desinfektio : geenimonistusmenetelmän luotettavuuden parantaminen. [Internet] [Masters thesis]. University of Helsinki; 2010. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/10138/16231.

Council of Science Editors:

Grönroos S. Noroviruksen desinfektio : geenimonistusmenetelmän luotettavuuden parantaminen. [Masters Thesis]. University of Helsinki; 2010. Available from: http://hdl.handle.net/10138/16231

University of Texas – Austin

21. Zhao, Meng. Genetic and biochemical studies on the differential modulation of RNA decay and processing by inhibitory proteins in Escherichia coli.

Degree: PhD, Cell and Molecular Biology, 2006, University of Texas – Austin

URL: http://hdl.handle.net/2152/3018

Subjects/Keywords: Messenger RNA; Ribonucleases; Escherichia coli – Genetics; Escherichia coli – Physiology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Zhao, M. (2006). Genetic and biochemical studies on the differential modulation of RNA decay and processing by inhibitory proteins in Escherichia coli. (Doctoral Dissertation). University of Texas – Austin. Retrieved from http://hdl.handle.net/2152/3018

Chicago Manual of Style (16^th Edition):

Zhao, Meng. “Genetic and biochemical studies on the differential modulation of RNA decay and processing by inhibitory proteins in Escherichia coli.” 2006. Doctoral Dissertation, University of Texas – Austin. Accessed January 18, 2021. http://hdl.handle.net/2152/3018.

MLA Handbook (7^th Edition):

Zhao, Meng. “Genetic and biochemical studies on the differential modulation of RNA decay and processing by inhibitory proteins in Escherichia coli.” 2006. Web. 18 Jan 2021.

Vancouver:

Zhao M. Genetic and biochemical studies on the differential modulation of RNA decay and processing by inhibitory proteins in Escherichia coli. [Internet] [Doctoral dissertation]. University of Texas – Austin; 2006. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/2152/3018.

Council of Science Editors:

Zhao M. Genetic and biochemical studies on the differential modulation of RNA decay and processing by inhibitory proteins in Escherichia coli. [Doctoral Dissertation]. University of Texas – Austin; 2006. Available from: http://hdl.handle.net/2152/3018

Aristotle University Of Thessaloniki (AUTH); Αριστοτέλειο Πανεπιστήμιο Θεσσαλονίκης (ΑΠΘ)

22. ΠΑΝΤΟΠΟΥΛΟΣ, ΚΩΝΣΤΑΝΤΙΝΟΣ. ΚΑΘΑΡΙΣΜΟΣ ΚΑΙ ΙΔΙΟΤΗΤΕΣ ΤΡΙΩΝ ΡΙΒΟΝΟΥΚΛΕΑΣΩΝ ΑΠΟ ΠΥΡΗΝΕΣ ΕΓΚΕΦΑΛΟΥ ΚΟΥΝΕΛΙΟΥ.

Degree: 1992, Aristotle University Of Thessaloniki (AUTH); Αριστοτέλειο Πανεπιστήμιο Θεσσαλονίκης (ΑΠΘ)

URL: http://hdl.handle.net/10442/hedi/2016

►

THE PURIFICATION AND PROPERTIES OF THREE RIBONUCLEASES FROM RABBIT BRAIN NUCLEIIS REPORTED IN THE PRESENT THESIS. THE ENZYMES, CALLED RIBONUCLEASES A1, A2 AND B RESPECTIVELY… (more)

▼

THE PURIFICATION AND PROPERTIES OF THREE RIBONUCLEASES FROM RABBIT BRAIN NUCLEIIS REPORTED IN THE PRESENT THESIS. THE ENZYMES, CALLED RIBONUCLEASES A1, A2 AND B RESPECTIVELY ARE EXTRACTED FROM NUCLEI BY MEANS OF 0.5 M NACL AND PURIFIED BY A SERIES OF ION EXCHANGE AND AFFINITY CHROMATOGRAPHY STEPS. THE RIBONUCLEASES DISPLAY OPTIMAL ACTIVITY AT NEAR NEUTRAL PH IN THE PRESENCE OF 50-80 MM NACL AND DO NOT REQUIRE BIVALENT IONS. THEY ARE ENDONUCLEASES AND THEIR HYDROLYSIS PRODUCTS BEAR A FREE 5' HYDROXYL GROUP, WHILE THE TERMINAL PHOSPHATE IN ENZYMES A1 AND A2 IS MONOESTERIFIED AT THE 3' POSITION AND IN ENZYME B IS DIESTERIFIED AT POSITIONS 2' AND 3'. IN ORDER TO ESTABLISH THEIR BASE SPECIFICITY, THE ENZYMES HAVE BEEN ALLOWED TO ACT ON DIFFERENT SUBSTRATES SUCH AS DINUCLEOSIDE MONOPHOSPHATES, DEPHOSPHORYLATED TRINUCLEOTIDES, HOMOPOLYRIBONUCLEOTIDES AND 5 S RNA FROM E. COLI. RNASE A1 CLEAVES PREFERENTIALLY APN BONDS, RNASE A2 WAS FOUND TO BE PYRIMIDINE SPECIFIC IN THAT IT PREFERENTIALLY CLEAVES YPN BONDS, AND RNASE BHYDROLYZES RPU BONDS. FURTHERMORE THE THREE ENZYMES POSSESS DIFFERENT IMMUNOLOGICAL PROPERTIES, SINCE ANTIBODIES RAISED AGAINST RNASE B DO NOT CROSSREACT WITH THE OTHER TWO RIBONUCLEASES.

ΣΤΗΝ ΕΡΓΑΣΙΑ ΑΥΤΗ ΑΠΟΜΟΝΩΘΗΚΑΝ ΤΡΕΙΣ ΝΕΕΣ ΡΙΒΟΝΟΥΚΛΕΑΣΕΣ ΑΠΟ ΠΥΡΗΝΕΣ ΕΓΚΕΦΑΛΟΥ ΚΟΥΝΕΛΙΟΥ, ΠΟΥ ΟΝΟΜΑΣΤΗΚΑΝ Α1, Α2 ΚΑΙ Β. ΤΑ ΕΝΖΥΜΑ ΕΚΧΥΛΙΣΤΗΚΑΝ ΑΠΟ ΤΟΥΣ ΠΥΡΗΝΕΣ ΜΕ 0.5 Μ ΝΑCL, ΚΑΙ ΚΑΘΑΡΙΣΤΗΚΑΝ ΜΕ ΜΙΑ ΣΕΙΡΑ ΧΡΩΜΑΤΟΓΡΑΦΙΚΑ ΣΤΑΔΙΑ ΙΟΝΙΚΗΣ ΑΝΤΑΛΛΑΓΗΣ ΚΑΙ ΑΓΧΙΣΤΕΙΑΣ. ΔΡΟΥΝ ΚΑΛΥΤΕΡΑ ΚΟΝΤΑ ΣΤΗΝ ΟΥΔΕΤΕΡΗ ΠΕΡΙΟΧΗ ΤΟΥ ΡΗ ΠΑΡΟΥΣΙΑ 50-80 ΜΜ ΝΑCL, ΚΑΙ ΔΕΝ ΑΠΑΙΤΟΥΝ ΔΙΣΘΕΝΗ ΙΟΝΤΑ. ΕΙΝΑΙ ΕΝΔΟΝΟΥΚΛΕΑΣΕΣ, ΚΑΙ ΤΑ ΠΡΟΙΟΝΤΑ ΥΔΡΟΛΥΣΗΣ ΕΧΟΥΝ ΕΛΕΥΘΕΡΟ 5' ΥΔΡΟΞΥΛΙΟ, ΕΝΩ Η ΑΚΡΑΙΑ ΦΩΣΦΟΡΙΚΗ ΟΜΑΔΑ ΣΤΑ ΜΕΝ ΕΝΖΥΜΑ Α1 ΚΑΙ Α2 ΕΙΝΑΙ ΜΟΝΟΕΣΤΕΡΟΠΟΙΗΜΕΝΗ ΣΤΗΝ 3' ΘΕΣΗ, ΣΤΗ ΔΕ ΡΙΒΟΝΟΥΚΛΕΑΣΗ Β ΕΙΝΑΙ ΚΥΚΛΙΚΑ ΔΙΕΣΤΕΡΟΠΟΙΗΜΕΝΗ ΣΤΙΣ 2' ΚΑΙ 3' ΘΕΣΕΙΣ. ΓΙΑ ΤΗ ΜΕΛΕΤΗ ΤΗΣΕΞΕΙΔΙΚΕΥΣΗΣ ΤΩΝ ΤΡΙΩΝ ΕΝΖΥΜΩΝ ΑΠΕΝΑΝΤΙ ΣΤΙΣ ΒΑΣΕΙΣ ΤΟΥ ΥΠΟΣΤΡΩΜΑΤΟΣ ΧΡΗΣΙΜΟΠΟΙΗΘΗΚΑΝ ΔΙΑΦΟΡΕΤΙΚΑ ΥΠΟΣΤΡΩΜΑΤΑ ΟΠΩΣ ΑΠΟΦΩΣΦΟΡΥΛΙΩΜΕΝΑ ΔΙ- ΚΑΙ ΤΡΙΝΟΥΚΛΕΟΤΙΔΙΑ,ΟΜΟΠΟΛΥΡΙΒΟΝΟΥΚΛΕΟΤΙΔΙΑ ΚΑΙ 5 S RNA ΑΠΟ E. COLI. ΒΡΕΘΗΚΕ ΟΤΙ Η ΡΙΒΟΝΟΥΚΛΕΑΣΗ Α1 ΥΔΡΟΛΥΕΙ ΔΕΣΜΟΥΣ ΑΡΝ, Η ΡΙΒΟΝΟΥΚΛΕΑΣΗ Α2 ΕΙΝΑΙ ΕΞΕΙΔΙΚΕΥΜΕΝΗ ΑΠΕΝΑΝΤΙ ΣΕ ΠΥΡΙΜΙΔΙΝΕΣ, ΚΑΙ ΥΔΡΟΛΥΕΙ ΔΕΣΜΟΥΣ ΥΡΝ, ΕΝΩ Η ΡΙΒΟΝΟΥΚΛΕΑΣΗ Β ΥΔΡΟΛΥΕΙ ΔΕΣΜΟΥΣ RPU. ΤΑ ΤΡΙΑ ΕΝΖΥΜΑ ΕΧΟΥΝ ΚΑΙ ΔΙΑΦΟΡΕΤΙΚΕΣ ΑΝΟΣΟΛΟΓΙΚΕΣ ΙΔΙΟΤΗΤΕΣ, ΚΑΘΩΣ ΑΝΤΙΣΩΜΑ ΑΠΕΝΑΝΤΙ ΣΤΗ ΡΙΒΟΝΟΥΚΛΕΑΣΗ Β ΔΕΝ ΑΝΤΙΔΡΑ ΜΕ ΤΙΣ ΑΛΛΕΣ ΡΙΒΟΝΟΥΚΛΕΑΣΕΣ.

Subjects/Keywords: Εγκέφαλος; ΝΟΥΚΛΕΑΣΕΣ; Πυρήνες; Ριβονουκλεάσες; Brain; NUCLEASES; NUCLEI; RIBONUCLEASES (RNASES)

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

ΠΑΝΤΟΠΟΥΛΟΣ, . �. (1992). ΚΑΘΑΡΙΣΜΟΣ ΚΑΙ ΙΔΙΟΤΗΤΕΣ ΤΡΙΩΝ ΡΙΒΟΝΟΥΚΛΕΑΣΩΝ ΑΠΟ ΠΥΡΗΝΕΣ ΕΓΚΕΦΑΛΟΥ ΚΟΥΝΕΛΙΟΥ. (Thesis). Aristotle University Of Thessaloniki (AUTH); Αριστοτέλειο Πανεπιστήμιο Θεσσαλονίκης (ΑΠΘ). Retrieved from http://hdl.handle.net/10442/hedi/2016

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

ΠΑΝΤΟΠΟΥΛΟΣ, ΚΩΝΣΤΑΝΤΙΝΟΣ. “ΚΑΘΑΡΙΣΜΟΣ ΚΑΙ ΙΔΙΟΤΗΤΕΣ ΤΡΙΩΝ ΡΙΒΟΝΟΥΚΛΕΑΣΩΝ ΑΠΟ ΠΥΡΗΝΕΣ ΕΓΚΕΦΑΛΟΥ ΚΟΥΝΕΛΙΟΥ.” 1992. Thesis, Aristotle University Of Thessaloniki (AUTH); Αριστοτέλειο Πανεπιστήμιο Θεσσαλονίκης (ΑΠΘ). Accessed January 18, 2021. http://hdl.handle.net/10442/hedi/2016.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

ΠΑΝΤΟΠΟΥΛΟΣ, ΚΩΝΣΤΑΝΤΙΝΟΣ. “ΚΑΘΑΡΙΣΜΟΣ ΚΑΙ ΙΔΙΟΤΗΤΕΣ ΤΡΙΩΝ ΡΙΒΟΝΟΥΚΛΕΑΣΩΝ ΑΠΟ ΠΥΡΗΝΕΣ ΕΓΚΕΦΑΛΟΥ ΚΟΥΝΕΛΙΟΥ.” 1992. Web. 18 Jan 2021.

Vancouver:

ΠΑΝΤΟΠΟΥΛΟΣ �. ΚΑΘΑΡΙΣΜΟΣ ΚΑΙ ΙΔΙΟΤΗΤΕΣ ΤΡΙΩΝ ΡΙΒΟΝΟΥΚΛΕΑΣΩΝ ΑΠΟ ΠΥΡΗΝΕΣ ΕΓΚΕΦΑΛΟΥ ΚΟΥΝΕΛΙΟΥ. [Internet] [Thesis]. Aristotle University Of Thessaloniki (AUTH); Αριστοτέλειο Πανεπιστήμιο Θεσσαλονίκης (ΑΠΘ); 1992. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/10442/hedi/2016.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

ΠΑΝΤΟΠΟΥΛΟΣ �. ΚΑΘΑΡΙΣΜΟΣ ΚΑΙ ΙΔΙΟΤΗΤΕΣ ΤΡΙΩΝ ΡΙΒΟΝΟΥΚΛΕΑΣΩΝ ΑΠΟ ΠΥΡΗΝΕΣ ΕΓΚΕΦΑΛΟΥ ΚΟΥΝΕΛΙΟΥ. [Thesis]. Aristotle University Of Thessaloniki (AUTH); Αριστοτέλειο Πανεπιστήμιο Θεσσαλονίκης (ΑΠΘ); 1992. Available from: http://hdl.handle.net/10442/hedi/2016

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universitat de Girona

23. García Galindo, Glòria. Anàlisi de la selectivitat per a cèl·lules tumorals de ribonucleases citotòxiques i desenvolupament d'eines per augmentar aquesta selectivitat.

Degree: Departament de Biologia, 2016, Universitat de Girona

URL: http://hdl.handle.net/10803/394027

► Ribonucleases are enzymes that exert selective cytotoxic for tumor cells. Previously, we proposed the use of nuclear-directed ribonucleases (ND-RNases) as antitumor drugs. In this work… (more)

Subjects/Keywords: Citotoxicitat; Citotoxicity; Citotoxicidad; Ribonucleases dirigides a nucli; Nuclear-directed ribonucleases; Ribonucleasas dirigidas a núcleo; Fàrmacs antitumorals; Antitumor drugs; Fármacos antitumorales; Selectivitat per a cèl·lules tumorals; Selective tumor cells; Selectividad para células tumorales; NES condicional; Conditional NES; Inhibidors CDK; CDK inhibitors; 577

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

García Galindo, G. (2016). Anàlisi de la selectivitat per a cèl·lules tumorals de ribonucleases citotòxiques i desenvolupament d'eines per augmentar aquesta selectivitat. (Thesis). Universitat de Girona. Retrieved from http://hdl.handle.net/10803/394027

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

García Galindo, Glòria. “Anàlisi de la selectivitat per a cèl·lules tumorals de ribonucleases citotòxiques i desenvolupament d'eines per augmentar aquesta selectivitat.” 2016. Thesis, Universitat de Girona. Accessed January 18, 2021. http://hdl.handle.net/10803/394027.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

García Galindo, Glòria. “Anàlisi de la selectivitat per a cèl·lules tumorals de ribonucleases citotòxiques i desenvolupament d'eines per augmentar aquesta selectivitat.” 2016. Web. 18 Jan 2021.

Vancouver:

García Galindo G. Anàlisi de la selectivitat per a cèl·lules tumorals de ribonucleases citotòxiques i desenvolupament d'eines per augmentar aquesta selectivitat. [Internet] [Thesis]. Universitat de Girona; 2016. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/10803/394027.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

García Galindo G. Anàlisi de la selectivitat per a cèl·lules tumorals de ribonucleases citotòxiques i desenvolupament d'eines per augmentar aquesta selectivitat. [Thesis]. Universitat de Girona; 2016. Available from: http://hdl.handle.net/10803/394027

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universitat de Girona

24. Coll Constans, M. Gràcia. Producció i caracterització de variants de la regió C-terminal de la ribonucleasa A. Importància d'aquesta regió sobre l'estabilitat de l'enzim.

Degree: Departament de Biologia, 2000, Universitat de Girona

URL: http://hdl.handle.net/10803/96338

► La ribonucleasa A de pàncrees boví (RNasa A, EC 3.1.27.5) ha estat extensament estudiada des de punts de vista estructurals, mecanístics i funcionals. Dins del… (more)

Subjects/Keywords: Ribonucleases; Ribonucléases; Enzims digestius; Digestive enzymes; Ribonucleasas; Enzimas digestivas; 57; 577

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APA (6^th Edition):

Coll Constans, M. G. (2000). Producció i caracterització de variants de la regió C-terminal de la ribonucleasa A. Importància d'aquesta regió sobre l'estabilitat de l'enzim. (Thesis). Universitat de Girona. Retrieved from http://hdl.handle.net/10803/96338

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Coll Constans, M Gràcia. “Producció i caracterització de variants de la regió C-terminal de la ribonucleasa A. Importància d'aquesta regió sobre l'estabilitat de l'enzim.” 2000. Thesis, Universitat de Girona. Accessed January 18, 2021. http://hdl.handle.net/10803/96338.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Coll Constans, M Gràcia. “Producció i caracterització de variants de la regió C-terminal de la ribonucleasa A. Importància d'aquesta regió sobre l'estabilitat de l'enzim.” 2000. Web. 18 Jan 2021.

Vancouver:

Coll Constans MG. Producció i caracterització de variants de la regió C-terminal de la ribonucleasa A. Importància d'aquesta regió sobre l'estabilitat de l'enzim. [Internet] [Thesis]. Universitat de Girona; 2000. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/10803/96338.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Coll Constans MG. Producció i caracterització de variants de la regió C-terminal de la ribonucleasa A. Importància d'aquesta regió sobre l'estabilitat de l'enzim. [Thesis]. Universitat de Girona; 2000. Available from: http://hdl.handle.net/10803/96338

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

East Tennessee State University

25. Silvers, Jessica A. Degradation of 23S rRNA in Azithromycin-Treated Ribonuclease Mutants of Escherichia coli.

Degree: MS, Biomedical Sciences, 2004, East Tennessee State University

URL: https://dc.etsu.edu/etd/957

► Azithromycin, a macrolide antibiotic, specifically binds to the 50S ribosomal subunit of bacterial ribosomes and inhibits translation. Azithromycin also prevents 50S ribosomal subunit assembly… (more)

Subjects/Keywords: ribosome; rRNA; azithromycin; 50S; 23S; ribonucleases; Medical Sciences; Medicine and Health Sciences

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Silvers, J. A. (2004). Degradation of 23S rRNA in Azithromycin-Treated Ribonuclease Mutants of Escherichia coli. (Thesis). East Tennessee State University. Retrieved from https://dc.etsu.edu/etd/957

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Silvers, Jessica A. “Degradation of 23S rRNA in Azithromycin-Treated Ribonuclease Mutants of Escherichia coli.” 2004. Thesis, East Tennessee State University. Accessed January 18, 2021. https://dc.etsu.edu/etd/957.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Silvers, Jessica A. “Degradation of 23S rRNA in Azithromycin-Treated Ribonuclease Mutants of Escherichia coli.” 2004. Web. 18 Jan 2021.

Vancouver:

Silvers JA. Degradation of 23S rRNA in Azithromycin-Treated Ribonuclease Mutants of Escherichia coli. [Internet] [Thesis]. East Tennessee State University; 2004. [cited 2021 Jan 18]. Available from: https://dc.etsu.edu/etd/957.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Silvers JA. Degradation of 23S rRNA in Azithromycin-Treated Ribonuclease Mutants of Escherichia coli. [Thesis]. East Tennessee State University; 2004. Available from: https://dc.etsu.edu/etd/957

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Lethbridge

26. University of Lethbridge. Faculty of Arts and Science. Structural and functional dynamics of Escherichia coli ribonuclease II : initial studies using a novel fluorescence based system .

Degree: 2009, University of Lethbridge

URL: http://hdl.handle.net/10133/1289

► Ribonuclease II (RNase II) is a bacterial enzyme responsible for 90% of the exonucleolytic degradation of mRNA in bacteria, and has bacterial homologues known to… (more)

Subjects/Keywords: Ribonucleases – Research; Escherichia coli – Research; Messenger RNA – Research; Dissertations, Academic

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Science, U. o. L. F. o. A. a. (2009). Structural and functional dynamics of Escherichia coli ribonuclease II : initial studies using a novel fluorescence based system . (Thesis). University of Lethbridge. Retrieved from http://hdl.handle.net/10133/1289

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Science, University of Lethbridge. Faculty of Arts and. “Structural and functional dynamics of Escherichia coli ribonuclease II : initial studies using a novel fluorescence based system .” 2009. Thesis, University of Lethbridge. Accessed January 18, 2021. http://hdl.handle.net/10133/1289.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Science, University of Lethbridge. Faculty of Arts and. “Structural and functional dynamics of Escherichia coli ribonuclease II : initial studies using a novel fluorescence based system .” 2009. Web. 18 Jan 2021.

Vancouver:

Science UoLFoAa. Structural and functional dynamics of Escherichia coli ribonuclease II : initial studies using a novel fluorescence based system . [Internet] [Thesis]. University of Lethbridge; 2009. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/10133/1289.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Science UoLFoAa. Structural and functional dynamics of Escherichia coli ribonuclease II : initial studies using a novel fluorescence based system . [Thesis]. University of Lethbridge; 2009. Available from: http://hdl.handle.net/10133/1289

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

27. ΜΑΥΡΟΘΑΛΑΣΣΙΤΗΣ, ΓΕΩΡΓΙΟΣ. ΚΑΘΑΡΙΣΜΟΣ ΚΑΙ ΙΔΙΟΤΗΤΕΣ ΤΡΙΩΝ ΚΥΤΤΑΡΟΠΛΑΣΜΑΤΙΚΩΝ ΡΙΒΟΝΟΥΚΛΕΑΣΩΝ ΑΠΟ ΣΥΚΩΤΙ ΠΟΝΤΙΚΟΥ.

Degree: 1987, Αριστοτέλειο Πανεπιστήμιο Θεσσαλονίκης (ΑΠΘ); Aristotle University Of Thessaloniki (AUTH)

URL: http://hdl.handle.net/10442/hedi/0605

►

AN ACIDIC, A NEUTRAL AND AN ALKALINE RIBONUCLEASE WERE ISOLATED FROM MOUSE LIVER CYTOPLASM, BY GEL FILTRATION, ION EXCHANGE AND AFFINITY CHROMATOGRAPHY. THE ACIDIC RIBONUCLEASE… (more)

▼

AN ACIDIC, A NEUTRAL AND AN ALKALINE RIBONUCLEASE WERE ISOLATED FROM MOUSE LIVER CYTOPLASM, BY GEL FILTRATION, ION EXCHANGE AND AFFINITY CHROMATOGRAPHY. THE ACIDIC RIBONUCLEASE HYDROLYSES RNA OPTIMALLY AT PH 6.2, IN THE ABSENCE OF SALTS AND AT PH 5.5 IN SOLUTIONS WITH 150 MM OF MONOVALENT SALTS. IT IS NOT AFFECTED BY MG+2, ATP AND EDTA, BUT IT IS INHIBITED BY CA+2 AND ZN+2. IT IS ACID RESISTANCE AND HEAT LABILE. IT HYDROLYSES POLYPYRIMIDINORIBONUCLEOTIDES AND RNA IN NP-U AND GPAP-G BONDS. THE NEUTRAL RIBONUCLEASE HAS OPTIMUM PH 7.5 AND NEEDS 100-200 MM OF MONOVALENT SALTS. IT IS NOT AFFECTED BY ATP AND EDTA AND IS INHIBITED BY MG+2, CA+2 AND ZN+2. IT IS ACID AND HEAT RESISTANT. IT HYDROLYSES POLYPYRIMIDINORIBONUCLEOTIDES AND PYRIMIDINE-PURINE AND PYRIMIDINE-PYRIMIDINE BONDS IN RNA. THE ALKALINE RIBONUCLEASE HAS OPTIMUM PH 8.5, IN THE ABSENCE OF SALTS AND PH7 IN BUFFERS WITH 150 MM MONOVALENT SALTS. IT IS NOT AFFECTED BY ATP AND EDTA AND IS INHIBITED BY MG+2, CA+2 AND ZN+2. IT IS ACID AND HEAT RESISTANT. IT HYDROLYSES POLYPYRIMIDINORIBONUCLEOTIDES AND MAINLY POLY(C), RNA IS HYDROLYSED BY THIS ENZYME, IN THE SAME WAY AS BY THE NEUTRAL AND THE PANKREATIC RNASE. ALL THREE ENZYMES ARE ENDONUCLEASES AND THEY PRODUCE 3' PHOSPHOMONOESTERS. THEY DO NOTHYDROLYSE SS OR DS DNA. IN THE ABSENCE OF MONOVALENT SALT, THEY ARE ATTACHED STRONGLY ON THE POLYSOMES.

ΑΠΟ ΤΟ ΚΥΤΤΑΡΟΠΛΑΣΜΑ ΣΥΚΩΤΙΟΥ ΠΟΝΤΙΚΟΥ, ΜΕ ΧΡΩΜΑΤΟΓΡΑΦΙΑ ΣΕ ΣΤΗΛΕΣ ΜΟΡΙΑΚΗΣ ΔΙΗΘΗΣΗΣ, ΙΟΝΙΚΗΣ ΑΝΤΑΛΛΑΓΗΣ ΚΑΙ ΑΓΧΙΣΤΕΙΑΣ, ΑΠΟΜΟΝΩΘΗΚΑΝ ΜΙΑ ΟΞΙΝΗ, ΜΙΑ ΟΥΔΕΤΕΡΗ ΚΑΙ ΜΙΑ ΑΛΚΑΛΙΚΗ ΡΙΒΟΝΟΥΚΛΕΑΣΗ. Η ΟΞΙΝΗ ΡΙΒΟΝΟΥΚΛΕΑΣΗ ΕΧΕΙ ΒΕΛΤΙΣΤΟ ΡΗ 6.2 ΑΠΟΥΣΙΑ ΑΛΑΤΩΝ ΚΑΙ ΡΗ 5.5 ΣΕ ΔΙΑΛΥΜΑΤΑ ΜΕ ΙΟΝΙΚΗ ΙΣΧΗ 150ΜΜ. ΔΕΝ ΕΠΗΡΕΑΖΕΤΑΙ ΑΠΟ ΤΟMG+2, ΤΟ ATP ΚΑΙ ΤΟ EDTA, ΑΛΛΑ ΑΝΑΣΤΕΛΛΕΤΑΙ ΑΠΟ ΤΟ CA+2 ΚΑΙ ΤΟΝ ZN+2. ΑΝΤΕΧΕΙ ΣΤΑ ΟΞΕΑ, ΑΛΛΑ ΚΑΤΑΣΤΡΕΦΕΤΑΙ ΜΕ ΤΗ ΘΕΡΜΑΝΣΗ ΣΤΟΥΣ 100 C ΓΙΑ 5'. ΥΔΡΟΛΥΕΙ ΤΑ ΠΟΛΥΠΥΡΙΜΙΔΙΝΟΡΙΒΟΝΟΥΚΛΕΟΤΙΔΙΑ, ΚΑΙ ΤΟ ΦΥΣΙΚΟ RNA ΣΤΟΥΣ ΔΕΣΜΟΥΣ NP-U ΚΑΙ GPAP-GP. Η ΟΥΔΕΤΕΡΗ ΡΙΒΟΝΟΥΚΛΕΑΣΗ ΕΧΕΙ ΒΕΛΤΙΣΤΟ ΡΗ ΔΡΑΣΗΣ 7.5 ΚΑΙ ΑΠΑΙΤΕΙ ΑΛΑΤΑ ΣΕ ΣΥΓΚΕΝΤΡΩΣΗ 100-200ΜΜ. ΔΕΝ ΕΠΗΡΕΑΖΕΤΑΙ ΑΠΟ ΤΟ MG+2, ATP ΚΑΙ EDTA, ΚΑΙ ΑΝΑΣΤΕΛΛΕΤΑΙ ΑΠΟ CA+2 KAI ZN+2. ΑΝΤΕΧΕΙ ΣΤΑ ΟΞΕΑ ΚΑΙ ΣΤΗ ΘΕΡΜΑΝΣΗ. ΥΔΡΟΛΥΕΙ ΤΑ ΠΟΛΥΠΥΡΙΜΙΔΙΝΟΡΙΒΟΝΟΥΚΛΕΟΤΙΔΙΑ ΚΑΙ ΤΟ ΦΥΣΙΚΟ RNA ΣΤΟΥΣ ΔΕΣΜΟΥΣ ΜΕΤΑΞΥ ΠΥΡΙΜΙΔΙΝΩΝ-ΠΟΥΡΙΝΩΝ ΚΥΡΙΩΣ, ΑΛΛΑ ΚΑΙ ΜΕΤΑΞΥ ΠΥΡΙΜΙΔΙΝΩΝ. Η ΑΛΚΑΛΙΚΗ ΡΙΒΟΝΟΥΚΛΕΑΣΗ ΕΧΕΙ ΒΕΛΤΙΣΤΟ PH 8.5 ΣΕ ΔΙΑΛΥΜΑΤΑ ΧΩΡΙΣ ΑΛΑΤΑ ΚΑΙ PH 7 ΣΕ ΔΙΑΛΥΜΑΤΑ ΜΕ 150ΜΜ ΑΛΑΤΑ. ΔΕΝ ΕΠΗΡΕΑΖΕΤΑΙ ΑΠΟ ΤΟ ATP ΚΑΙ ΤΟ EDTA, ΑΛΛΑ ΑΝΑΣΤΕΛΛΕΤΑΙ ΑΠΟ MG+2, CA+2 ΚΑΙ ΖΝ+2. ΑΝΤΕΧΕΙ ΣΤΑ ΟΞΕΑ ΚΑΙ ΣΤΗ ΘΕΡΜΑΝΣΗ. ΥΔΡΟΛΥΕΙ ΠΟΛΥΠΥΡΙΜΙΔΙΝΟΡΙΒΟΝΟΥΚΛΕΟΤΙΔΙΑ ΚΑΙ ΚΥΡΙΩΣ POLY(C), ΕΝΩ ΤΟ RNA ΥΔΡΟΛΥΕΤΑΙ, ΟΠΩΣ ΚΑΙ ΑΠΟ ΤΟ ΟΥΔΕΤΕΡΟ ΕΝΖΥΜΟ ΚΑΙ ΤΗΝ ΚΡΥΣΤΑΛΛΙΚΗ ΠΑΓΚΡΕΑΤΙΚΗ ΡΙΒΟΝΟΥΚΛΕΑΣΗ. ΟΛΑ ΤΑ ΕΝΖΥΜΑ ΕΙΝΑΙ ΕΝΔΟΝΟΥΚΛΕΑΣΕΣ, ΑΦΗΝΟΥΝ ΤΗ ΦΩΣΦΟΡΙΚΗ ΟΜΑΔΑ ΣΤΗΝ 3' ΘΕΣΗ ΤΩΝ ΟΛΙΓΟΝΟΥΚΛΕΟΤΙΔΙΩΝ ΠΟΥ ΔΗΜΙΟΥΡΓΟΥΝΤΑΙ ΚΑΙ ΠΑΡΟΥΣΙΑΖΟΥΝ ΠΛΗΡΗ ΑΔΥΝΑΜΙΑ ΝΑ ΥΔΡΟΛΥΣΟΥΝ ΜΟΝΟΚΛΩΝΟ 'Η ΔΙΚΛΩΝΟ DNA.ΣΕ ΑΛΚΑΛΙΚΟ ΠΕΡΙΒΑΛΛΟΝ, ΑΠΟΥΣΙΑ ΑΛΑΤΩΝ, ΣΥΝΔΕΟΝΤΑΙ ΙΣΧΥΡΑ ΜΕ ΤΑ ΠΟΛΥΣΩΜΑΤΙΑ.

Subjects/Keywords: CYTOCOLIC; PROPERTIES; Purification; Ribonucleases; SEQUENCE SPECIBICITY; ΕΞΕΙΔΗΚΕΥΣΗ ΑΛΛΗΛΟΥΧΙΑΣ; ΙΔΙΟΤΗΤΕΣ; Καθαρισμός; ΚΥΤΤΑΡΟΠΛΑΣΜΑΤΙΚΕΣ; Ριβονουκλεάσες

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

ΜΑΥΡΟΘΑΛΑΣΣΙΤΗΣ, �. (1987). ΚΑΘΑΡΙΣΜΟΣ ΚΑΙ ΙΔΙΟΤΗΤΕΣ ΤΡΙΩΝ ΚΥΤΤΑΡΟΠΛΑΣΜΑΤΙΚΩΝ ΡΙΒΟΝΟΥΚΛΕΑΣΩΝ ΑΠΟ ΣΥΚΩΤΙ ΠΟΝΤΙΚΟΥ. (Thesis). Αριστοτέλειο Πανεπιστήμιο Θεσσαλονίκης (ΑΠΘ); Aristotle University Of Thessaloniki (AUTH). Retrieved from http://hdl.handle.net/10442/hedi/0605

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

ΜΑΥΡΟΘΑΛΑΣΣΙΤΗΣ, ΓΕΩΡΓΙΟΣ. “ΚΑΘΑΡΙΣΜΟΣ ΚΑΙ ΙΔΙΟΤΗΤΕΣ ΤΡΙΩΝ ΚΥΤΤΑΡΟΠΛΑΣΜΑΤΙΚΩΝ ΡΙΒΟΝΟΥΚΛΕΑΣΩΝ ΑΠΟ ΣΥΚΩΤΙ ΠΟΝΤΙΚΟΥ.” 1987. Thesis, Αριστοτέλειο Πανεπιστήμιο Θεσσαλονίκης (ΑΠΘ); Aristotle University Of Thessaloniki (AUTH). Accessed January 18, 2021. http://hdl.handle.net/10442/hedi/0605.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

ΜΑΥΡΟΘΑΛΑΣΣΙΤΗΣ, ΓΕΩΡΓΙΟΣ. “ΚΑΘΑΡΙΣΜΟΣ ΚΑΙ ΙΔΙΟΤΗΤΕΣ ΤΡΙΩΝ ΚΥΤΤΑΡΟΠΛΑΣΜΑΤΙΚΩΝ ΡΙΒΟΝΟΥΚΛΕΑΣΩΝ ΑΠΟ ΣΥΚΩΤΙ ΠΟΝΤΙΚΟΥ.” 1987. Web. 18 Jan 2021.

Vancouver:

ΜΑΥΡΟΘΑΛΑΣΣΙΤΗΣ �. ΚΑΘΑΡΙΣΜΟΣ ΚΑΙ ΙΔΙΟΤΗΤΕΣ ΤΡΙΩΝ ΚΥΤΤΑΡΟΠΛΑΣΜΑΤΙΚΩΝ ΡΙΒΟΝΟΥΚΛΕΑΣΩΝ ΑΠΟ ΣΥΚΩΤΙ ΠΟΝΤΙΚΟΥ. [Internet] [Thesis]. Αριστοτέλειο Πανεπιστήμιο Θεσσαλονίκης (ΑΠΘ); Aristotle University Of Thessaloniki (AUTH); 1987. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/10442/hedi/0605.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

ΜΑΥΡΟΘΑΛΑΣΣΙΤΗΣ �. ΚΑΘΑΡΙΣΜΟΣ ΚΑΙ ΙΔΙΟΤΗΤΕΣ ΤΡΙΩΝ ΚΥΤΤΑΡΟΠΛΑΣΜΑΤΙΚΩΝ ΡΙΒΟΝΟΥΚΛΕΑΣΩΝ ΑΠΟ ΣΥΚΩΤΙ ΠΟΝΤΙΚΟΥ. [Thesis]. Αριστοτέλειο Πανεπιστήμιο Θεσσαλονίκης (ΑΠΘ); Aristotle University Of Thessaloniki (AUTH); 1987. Available from: http://hdl.handle.net/10442/hedi/0605

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Aristotle University Of Thessaloniki (AUTH); Αριστοτέλειο Πανεπιστήμιο Θεσσαλονίκης (ΑΠΘ)

28. Τζοΐτου, Μαριάνθη. Ο ρόλος της πρωτεΐνης p150 ως καρκινικού δείκτη σε πειραματικούς όγκους in vitro και σε ασθενείς με καρκίνο.

Degree: 2001, Aristotle University Of Thessaloniki (AUTH); Αριστοτέλειο Πανεπιστήμιο Θεσσαλονίκης (ΑΠΘ)

URL: http://hdl.handle.net/10442/hedi/22775

Subjects/Keywords: Καρκινικοί δείκτες; Καρκίνος μαστού; Καρκίνος πνεύμονα; Ριβονουκλεάσες; Λεκτίνες; p150; Cancer markers; Breast cancer; Lung cancer; Ribonucleases; Lectins

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Τζοΐτου, . �. (2001). Ο ρόλος της πρωτεΐνης p150 ως καρκινικού δείκτη σε πειραματικούς όγκους in vitro και σε ασθενείς με καρκίνο. (Thesis). Aristotle University Of Thessaloniki (AUTH); Αριστοτέλειο Πανεπιστήμιο Θεσσαλονίκης (ΑΠΘ). Retrieved from http://hdl.handle.net/10442/hedi/22775

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Τζοΐτου, Μαριάνθη. “Ο ρόλος της πρωτεΐνης p150 ως καρκινικού δείκτη σε πειραματικούς όγκους in vitro και σε ασθενείς με καρκίνο.” 2001. Thesis, Aristotle University Of Thessaloniki (AUTH); Αριστοτέλειο Πανεπιστήμιο Θεσσαλονίκης (ΑΠΘ). Accessed January 18, 2021. http://hdl.handle.net/10442/hedi/22775.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Τζοΐτου, Μαριάνθη. “Ο ρόλος της πρωτεΐνης p150 ως καρκινικού δείκτη σε πειραματικούς όγκους in vitro και σε ασθενείς με καρκίνο.” 2001. Web. 18 Jan 2021.

Vancouver:

Τζοΐτου �. Ο ρόλος της πρωτεΐνης p150 ως καρκινικού δείκτη σε πειραματικούς όγκους in vitro και σε ασθενείς με καρκίνο. [Internet] [Thesis]. Aristotle University Of Thessaloniki (AUTH); Αριστοτέλειο Πανεπιστήμιο Θεσσαλονίκης (ΑΠΘ); 2001. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/10442/hedi/22775.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Τζοΐτου �. Ο ρόλος της πρωτεΐνης p150 ως καρκινικού δείκτη σε πειραματικούς όγκους in vitro και σε ασθενείς με καρκίνο. [Thesis]. Aristotle University Of Thessaloniki (AUTH); Αριστοτέλειο Πανεπιστήμιο Θεσσαλονίκης (ΑΠΘ); 2001. Available from: http://hdl.handle.net/10442/hedi/22775

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Indian Institute of Science

29. Sanjeev, B S. Computational Studies On Eosinophil Associated Ribonucleases : Insights Into Dynamics And Catalysis Through Molecular Dynamics Simulations.

Degree: PhD, Faculty of Science, 2011, Indian Institute of Science

URL: http://etd.iisc.ac.in/handle/2005/1187

Subjects/Keywords: Eosinophil; Ribonucleases - Molecular Dynamics; Catalysis; Molecular Dynamics - Data Processing; RNase A; Angiogenin; Ribonuclease A; Cell Biology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sanjeev, B. S. (2011). Computational Studies On Eosinophil Associated Ribonucleases : Insights Into Dynamics And Catalysis Through Molecular Dynamics Simulations. (Doctoral Dissertation). Indian Institute of Science. Retrieved from http://etd.iisc.ac.in/handle/2005/1187

Chicago Manual of Style (16^th Edition):

Sanjeev, B S. “Computational Studies On Eosinophil Associated Ribonucleases : Insights Into Dynamics And Catalysis Through Molecular Dynamics Simulations.” 2011. Doctoral Dissertation, Indian Institute of Science. Accessed January 18, 2021. http://etd.iisc.ac.in/handle/2005/1187.

MLA Handbook (7^th Edition):

Sanjeev, B S. “Computational Studies On Eosinophil Associated Ribonucleases : Insights Into Dynamics And Catalysis Through Molecular Dynamics Simulations.” 2011. Web. 18 Jan 2021.

Vancouver:

Sanjeev BS. Computational Studies On Eosinophil Associated Ribonucleases : Insights Into Dynamics And Catalysis Through Molecular Dynamics Simulations. [Internet] [Doctoral dissertation]. Indian Institute of Science; 2011. [cited 2021 Jan 18]. Available from: http://etd.iisc.ac.in/handle/2005/1187.

Council of Science Editors:

Sanjeev BS. Computational Studies On Eosinophil Associated Ribonucleases : Insights Into Dynamics And Catalysis Through Molecular Dynamics Simulations. [Doctoral Dissertation]. Indian Institute of Science; 2011. Available from: http://etd.iisc.ac.in/handle/2005/1187

30. Salazar Montoya, Vivian Angélica. Exploring the mechanism of action of human antimicrobial ribonucleases.

Degree: Departament de Bioquímica i Biologia Molecular, 2015, Universitat Autònoma de Barcelona

URL: http://hdl.handle.net/10803/310611

► Human ribonucleases are a heterogeneous group of proteins belonging to the superfamily of RNase A. These proteins are characterized by their ability to hydrolyse ribonucleic… (more)

▼ Human ribonucleases are a heterogeneous group of proteins belonging to the superfamily of RNase A. These proteins are characterized by their ability to hydrolyse ribonucleic acids and the presence of antimicrobial activity against various pathogens including bacteria, fungi, parasites and viruses. The first objective of this doctoral study is focused on the antimicrobial characterization of native Ribonuclease 3 forms purified from eosinophils. Native forms present posttranslational modifications giving different glycosylation grades that modulate their activity during inflammatory processes. This study aims to establish the antimicrobial properties of native forms purified from eosinophils and their activity in a membrane model system. Results indicate that post-translational modifications contribute to the the protein biological activities, suggesting a related physiological role. As a second objective, we evaluated for the first time the antifungal activity of the antimicrobial RNase 3 and RNase 7 against Candida albicans, an eukaryotic pathogen selected as a simple model to test the antimicrobial mechanism of action. Both human ribonucleases displayed a high antifungal activity. Results highlighted a dual mechanism of action, where cell lysis takes place at high protein concentration, while depolarization, cell internalization and cellular RNA degradation is achieved at sublethal doses. Finally, the last objective is focused on the characterization of ribonuclease 8, also called the placental RNase, the most recent human ribonuclease described. RNase 8 has gained and lost one cysteine residue in non-conserved positions in a mechanism called "disulphide shuffling". The protein tendency to aggregate required the design of an alternative purification protocol. We analysed its antimicrobial abilities, suggesting a possible role in innate defence. The results of this study confirmed the high antimicrobial activity of several human ribonucleases from the RNase A superfamily suggesting an ancestral role in the host immune defence response. The study contributed to the understanding of their mechanism of action and set the basis for applied drug design. Advisors/Committee Members: [email protected] (authoremail), true (authoremailshow), Boix Borras, Ester (director), Moussaoui, Mohamed (director), true (authorsendemail).

Subjects/Keywords: Proteinas; Proteins; Ribonucleasas; Ribonucleases; Inmunidad innata; Host defence; Ciències Experimentals; 577

…1,2-diamine TFA Trifluoroacetic acid i RESUMEN Las ribonucleases humanas son un grupo… …plantea su potencial uso como herramientas terapéuticas. iii SUMMARY Human ribonucleases are… …ribonucleases displayed antifungal activity. Additionally, active site mutants of both RNases were… …antimicrobial activity of several human ribonucleases from the RNase A superfamily suggesting an… …acids. Besides, all ribonucleases of this family share a typical structural fold and the key…

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Salazar Montoya, V. A. (2015). Exploring the mechanism of action of human antimicrobial ribonucleases. (Thesis). Universitat Autònoma de Barcelona. Retrieved from http://hdl.handle.net/10803/310611

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Salazar Montoya, Vivian Angélica. “Exploring the mechanism of action of human antimicrobial ribonucleases.” 2015. Thesis, Universitat Autònoma de Barcelona. Accessed January 18, 2021. http://hdl.handle.net/10803/310611.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Salazar Montoya, Vivian Angélica. “Exploring the mechanism of action of human antimicrobial ribonucleases.” 2015. Web. 18 Jan 2021.

Vancouver:

Salazar Montoya VA. Exploring the mechanism of action of human antimicrobial ribonucleases. [Internet] [Thesis]. Universitat Autònoma de Barcelona; 2015. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/10803/310611.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Salazar Montoya VA. Exploring the mechanism of action of human antimicrobial ribonucleases. [Thesis]. Universitat Autònoma de Barcelona; 2015. Available from: http://hdl.handle.net/10803/310611

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Open Access Theses and Dissertations