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AUT University
1.
Nair, Smriti.
Towards the analysis of Potyvirus VPg Interacting Protein (PVIP) gene expression in response to potyvirus infection
.
Degree: 2013, AUT University
URL: http://hdl.handle.net/10292/5233 
► Potyvirus is the largest genus in the RNA plant virus family of Potyviridae. Potyviruses infect most of the economically important agricultural and ornamental crops; Dasheen…
(more)
▼ Potyvirus is the largest genus in the RNA plant virus family of Potyviridae. Potyviruses infect most of the economically important agricultural and ornamental crops; Dasheen mosaic virus (DsMV) is a potyvirus which infects edible aroid plants such as taro, particularly in the South Pacific region. These viruses rely on various host plant proteins for their movement and replication. One such plant protein which is known to interact with the viral protein called virus genome linked protein (VPg) is potyvirus VPg interacting protein (PVIP). Dunoyer et al., (2004) suggested role for PVIP in potyvirus movement, in disease development and as an important factor for virus replication. Further, through bioinformatics sequence analysis of homologous Arabidopsis thaliana and Nicotiana benthamiana PVIP sequences, the PVIP gene was found to be homologous to OBERON 1 and OBERON 2 in A. thaliana, suggesting a role for PVIP in meristem maintenance (Saiga et al, 2008; Anand, 2010). This analysis also suggested that PVIP may have a role independent of virus infection, it may be an important factor in plant development. Anand (2010) analysed the effect of abiotic stress on PVIP mRNA accumulation. The PVIP mRNA levels were assessed in leaf tissue of N. benthamiana under various dark and light conditions. A decline in the PVIP mRNA accumulation was observed when these plants were placed in continuous dark, suggesting that light induces the expression of PVIP mRNA. This study concluded that PVIP gene is responsive to this type of abiotic stress; however, the responsiveness of PVIP mRNA level to biotic stress such as virus infection is yet to be unravelled.
The aim of this study was to determine the variation of PVIP mRNA accumulation in healthy and DsMV infected taro using reverse transcriptase quantitative polymerase chain reaction (RT-qPCR). To conduct this analysis, the first objective was to identify appropriate
reference genes for use in virus infected studies in taro.
There are several methods to conduct gene quantification studies such as northern hybridization, microarray data analysis; among these RT-qPCR has become the most reliable, common and sensitive method for the quantification of gene expression. According to the MIQE guidelines, there are many factors that need to be considered while conducting RT-qPCR analysis, one such important variable is the use of appropriate
reference genes (Bustin et al., 2010). A
reference gene is defined as having a stable expression under different experimental treatments (Taylor et al., 2011). Housekeeping
genes such as glyceraldehydes-3-phosphate dehdrogenase (GAPDH), ubiquitin (UBQ), actin 18S rRNA have been used as
reference genes; however, it has been found that the expression level of many of these housekeeping
genes vary under various experimental conditions.
Lilly et al., (2011) assessed the stability of 12 candidate
reference genes in virus infected A. thaliana. Among these 12
genes studied such as actin, UBQ, only four
genes were shown to have stable expression. These…
Advisors/Committee Members: Higgins, Colleen (advisor).
Subjects/Keywords: Reference genes for real- time PCR;
Taro and Nicotiana benthamiana;
Primers for reference genes for quantitative PCR in monocots and dicots
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APA (6th Edition):
Nair, S. (2013). Towards the analysis of Potyvirus VPg Interacting Protein (PVIP) gene expression in response to potyvirus infection
. (Thesis). AUT University. Retrieved from http://hdl.handle.net/10292/5233
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Nair, Smriti. “Towards the analysis of Potyvirus VPg Interacting Protein (PVIP) gene expression in response to potyvirus infection
.” 2013. Thesis, AUT University. Accessed March 03, 2021.
http://hdl.handle.net/10292/5233.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Nair, Smriti. “Towards the analysis of Potyvirus VPg Interacting Protein (PVIP) gene expression in response to potyvirus infection
.” 2013. Web. 03 Mar 2021.
Vancouver:
Nair S. Towards the analysis of Potyvirus VPg Interacting Protein (PVIP) gene expression in response to potyvirus infection
. [Internet] [Thesis]. AUT University; 2013. [cited 2021 Mar 03].
Available from: http://hdl.handle.net/10292/5233.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Nair S. Towards the analysis of Potyvirus VPg Interacting Protein (PVIP) gene expression in response to potyvirus infection
. [Thesis]. AUT University; 2013. Available from: http://hdl.handle.net/10292/5233
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Universiteit Utrecht
2.
Heuvel, D.
Reference Genes for the Canine Intervertebral Disc: essential knowledge for gene expression research.
Degree: 2013, Universiteit Utrecht
URL: http://dspace.library.uu.nl:8080/handle/1874/285300
► Intervertebral disc (IVD) disease is a disease common to dogs, especially chondrodystrophic dogs (dogs with short legs compared to their body height). In IVD disease…
(more)
▼ Intervertebral disc (IVD) disease is a disease common to dogs, especially chondrodystrophic dogs (dogs with short legs compared to their body height). In IVD disease the tissues of the intervertebral disc are degenerating which leads to loss of function and degeneration.
To find a cure for the disease this study focuses on the molecular pathways playing an important role in IVD disease, which is done by determining a set of 15
reference genes which can be used in further studies.
These
reference genes will be assessed by using the quantitative PCR (qPCR) method with a SYBRgreen dye.
A total of 42 samples were used from dogs of different breed and disease state (diseased/healthy), from which 39 samples remained for analysis.
The qPCR data were analysed using the software programs geNorm and NormFinder.
Results from the analysis showed that the combination of RPS19, SPRP, HPRT and YWHAZ would be the best choice when looking at the whole IVD.
Advisors/Committee Members: Penning, dr. L. C., Smolders, L. A..
Subjects/Keywords: Diergeneeskunde; IVD disease; reference genes; intervertebral disc; canine; gene expression; qPCR
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APA (6th Edition):
Heuvel, D. (2013). Reference Genes for the Canine Intervertebral Disc: essential knowledge for gene expression research. (Masters Thesis). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/285300
Chicago Manual of Style (16th Edition):
Heuvel, D. “Reference Genes for the Canine Intervertebral Disc: essential knowledge for gene expression research.” 2013. Masters Thesis, Universiteit Utrecht. Accessed March 03, 2021.
http://dspace.library.uu.nl:8080/handle/1874/285300.
MLA Handbook (7th Edition):
Heuvel, D. “Reference Genes for the Canine Intervertebral Disc: essential knowledge for gene expression research.” 2013. Web. 03 Mar 2021.
Vancouver:
Heuvel D. Reference Genes for the Canine Intervertebral Disc: essential knowledge for gene expression research. [Internet] [Masters thesis]. Universiteit Utrecht; 2013. [cited 2021 Mar 03].
Available from: http://dspace.library.uu.nl:8080/handle/1874/285300.
Council of Science Editors:
Heuvel D. Reference Genes for the Canine Intervertebral Disc: essential knowledge for gene expression research. [Masters Thesis]. Universiteit Utrecht; 2013. Available from: http://dspace.library.uu.nl:8080/handle/1874/285300
Virginia Commonwealth University
3.
Jurmain, Jessica L.
Validation of Ninein as an Ethanol-related Quantitative Trait Gene: Reassessment, Design, and Functional Validation of Reference Genes for qPCR Analysis of Brain Tissue in Mice.
Degree: MS, Pharmacology & Toxicology, 2020, Virginia Commonwealth University
URL: https://doi.org/10.25772/V9RF-CX53
;
https://scholarscompass.vcu.edu/etd/6270
► The increasing use of quantitative real-time polymerase chain reaction (qPCR) as a method for quantifying gene expression has led to an increased demand for…
(more)
▼ The increasing use of quantitative real-time polymerase chain reaction (qPCR) as a method for quantifying gene expression has led to an increased demand for standardization of data analysis methods to ensure accurate reporting and robust, reproducible results. The exponential nature of qPCR amplification results in the potential magnification of what are usually very small sources of error. Relative gene expression calculations circumvent this issue by normalizing target gene expression data to within-sample expression of a previously validated, stably expressed
reference gene or
genes. Multiple studies discussed herein have found that qPCR data are more reliable and reproducible when multiple
reference genes are used, and that they are validated prior to use in experiments with new conditions. In this thesis, existing
reference genes are evaluated to ensure they meet these criteria in experimental paradigms used frequently in our laboratory. Existing work on ethanol’s anxiolytic-like effects in our laboratory utilized microarrays to identify
Ninein as a cis-regulated, quantitative trait gene for these effects in nucleus accumbens (NAc) of BXD recombinant inbred mice and their progenitors, C57BL6/J (B6) and DBA/2J (D2) mice. Contrasting behavioral responses to ethanol in these mouse strains make them a frequent
subject of study for determining genetic components underlying those behaviors. In the first data chapter, the case is made for eliminating one
reference gene typically used for qPCR data normalization in qPCR experiments assessing strain differences in NAc gene expression in the laboratory,
Ppp2r2a. The
reference genes subsequently validated for use in qPCR analysis in ethanol-naïve NAc and amygdala of saline and ethanol-treated B6 and D2 mice are then used in an in-depth characterization of
Ninein expression in B6 and D2 NAc and amygdala. Furthermore, evidence is provided for the first
in vivo observation of murine
Ninein transcript variant 6 in adult neural tissue. The data presented make the case for a more thorough re-evaluation of
reference genes for future qPCR experiments in the laboratory, as well as a potential mechanism for
Ninein’s involvement in variation of anxiolytic-like responses to ethanol in B6 and D2 mice.
Advisors/Committee Members: Dr. Jennifer Wolstenholme.
Subjects/Keywords: qPCR; reference genes; ethanol; Ninein; mice; Behavioral Neurobiology; Molecular Genetics; Other Genetics and Genomics
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APA (6th Edition):
Jurmain, J. L. (2020). Validation of Ninein as an Ethanol-related Quantitative Trait Gene: Reassessment, Design, and Functional Validation of Reference Genes for qPCR Analysis of Brain Tissue in Mice. (Thesis). Virginia Commonwealth University. Retrieved from https://doi.org/10.25772/V9RF-CX53 ; https://scholarscompass.vcu.edu/etd/6270
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Jurmain, Jessica L. “Validation of Ninein as an Ethanol-related Quantitative Trait Gene: Reassessment, Design, and Functional Validation of Reference Genes for qPCR Analysis of Brain Tissue in Mice.” 2020. Thesis, Virginia Commonwealth University. Accessed March 03, 2021.
https://doi.org/10.25772/V9RF-CX53 ; https://scholarscompass.vcu.edu/etd/6270.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Jurmain, Jessica L. “Validation of Ninein as an Ethanol-related Quantitative Trait Gene: Reassessment, Design, and Functional Validation of Reference Genes for qPCR Analysis of Brain Tissue in Mice.” 2020. Web. 03 Mar 2021.
Vancouver:
Jurmain JL. Validation of Ninein as an Ethanol-related Quantitative Trait Gene: Reassessment, Design, and Functional Validation of Reference Genes for qPCR Analysis of Brain Tissue in Mice. [Internet] [Thesis]. Virginia Commonwealth University; 2020. [cited 2021 Mar 03].
Available from: https://doi.org/10.25772/V9RF-CX53 ; https://scholarscompass.vcu.edu/etd/6270.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Jurmain JL. Validation of Ninein as an Ethanol-related Quantitative Trait Gene: Reassessment, Design, and Functional Validation of Reference Genes for qPCR Analysis of Brain Tissue in Mice. [Thesis]. Virginia Commonwealth University; 2020. Available from: https://doi.org/10.25772/V9RF-CX53 ; https://scholarscompass.vcu.edu/etd/6270
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
4.
Washington Luiz Gomes Costa.
AnÃlise espacial e temporal da expressÃo de genes relacionados ao metabolismo de lipÃdios em sementes de pinhÃo manso (Jatropha curcas L.).
Degree: Master, 2013, Universidade Federal do Ceará
URL: http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=9438
;
► O esgotamento das reservas de energia nÃo-renovÃvel, como petrÃleo, carvÃo e gÃs natural, tÃm estimulado a busca por fontes alternativas geradoras de energia. Neste contexto,…
(more)
▼ O esgotamento das reservas de energia nÃo-renovÃvel, como petrÃleo, carvÃo e gÃs natural, tÃm estimulado a busca por fontes alternativas geradoras de energia. Neste contexto, o pinhÃo manso (Jatropha curcas L.) tem recebido especial atenÃÃo por parte dos pesquisadores, devido à presenÃa de grande quantidade de Ãleo em suas sementes, que pode ser convertida em biodiesel. O objetivo deste trabalho foi investigar o comportamento de genes relacionados ao metabolismo de lipÃdios durante os processos de desenvolvimento e germinaÃÃo da semente de pinhÃo manso. Para quantificar com exatidÃo os nÃveis de expressÃo gÃnica por RT-qPCR foi feita uma seleÃÃo entre nove candidatos a genes de referÃncia. Nossos resultados mostraram que na anÃlise de sementes em desenvolvimento, os genes GAPDH, UCP, ACT11, PP2A2 e CICLOF foram os mais estÃveis. Para as sementes em germinaÃÃo, os genes considerados mais estÃveis foram EF1-α, PP2A2, GAPDH, PUB3 e ACT11. Para validar nossos resultados com genes de referÃncia, foi utilizado o padrÃo de expressÃo do gene que codifica a proteÃna oleosina no qual foi observado que o mesmo foi similar aos observados em artigos cientÃficos pesquisados, indicando que os genes de referÃncia estavam apropriados para normalizaÃÃo dos dados de RT-qPCR. ApÃs obtenÃÃo desses dados, efetuou-se um estudo da expressÃo por RT-qPCR de 20 genes envolvidos com o metabolismo de lipÃdios. Nossos resultados revelaram que os genes oleosina, β-cetoacil-ACP Sintase I e II, tioesterase A e triacilglicerol lipase I, bem como outros genes envolvidos na biossÃntese de lipÃdios, alcanÃaram altos nÃveis de expressÃo no desenvolvimento da semente. Os genes acil-CoA sintetase, tiolase e triacilglicerol lipase II, relacionados com a degradaÃÃo de lipÃdios apresentaram altos nÃveis de transcritos na germinaÃÃo da semente. Os dados obtidos neste trabalho contribuem para o entendimento das vias metabÃlicas estudadas, fornecendo subsÃdios para a produÃÃo, via engenharia genÃtica, de variedades melhoradas do pinhÃo manso.
The depletion of non-renewable energy such as oil, coal and natural gas, has stimulated the search for alternative sources of energy generation. In this context, physic nut (Jatropha curcas L.) has received special attention from researchers due to the presence of large amounts of oil in its seeds that can be converted into biodiesel. The objective of this study was to investigate the behavior of genes related to lipid metabolism during the development and germination of physic nut seeds. To accurately quantify the levels of gene expression by RT-qPCR, a selection of nine candidates for reference genes was performed. Our results showed that the GAPDH, UCP, ACT11, PP2A2 and CICLOF were the most stable genes during the development of the seeds. In germinating seeds, EF1-α, PP2A2, GAPDH, PUB3 and ACT11 were considered the most stable genes. To validate our findings with reference genes, we used the expression profile of the gene encoding the oleosin protein in which that was similar to those observed in the…
Advisors/Committee Members: FÃbio CÃsar Sousa Nogueira, Francisco de Assis de Paiva Campos, Josà HÃlio Costa, Fabiano de Moura Teixeira.
Subjects/Keywords: gene expression; normalizaÃÃo; genes de referÃncia; expressÃo gÃnica; BIOQUIMICA; reference genes; normalization; PinhÃo-manso - Semente; Sementes oleaginosas; RegulaÃÃo de expressÃo gÃnica; Genes
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APA (6th Edition):
Costa, W. L. G. (2013). AnÃlise espacial e temporal da expressÃo de genes relacionados ao metabolismo de lipÃdios em sementes de pinhÃo manso (Jatropha curcas L.). (Masters Thesis). Universidade Federal do Ceará. Retrieved from http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=9438 ;
Chicago Manual of Style (16th Edition):
Costa, Washington Luiz Gomes. “AnÃlise espacial e temporal da expressÃo de genes relacionados ao metabolismo de lipÃdios em sementes de pinhÃo manso (Jatropha curcas L.).” 2013. Masters Thesis, Universidade Federal do Ceará. Accessed March 03, 2021.
http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=9438 ;.
MLA Handbook (7th Edition):
Costa, Washington Luiz Gomes. “AnÃlise espacial e temporal da expressÃo de genes relacionados ao metabolismo de lipÃdios em sementes de pinhÃo manso (Jatropha curcas L.).” 2013. Web. 03 Mar 2021.
Vancouver:
Costa WLG. AnÃlise espacial e temporal da expressÃo de genes relacionados ao metabolismo de lipÃdios em sementes de pinhÃo manso (Jatropha curcas L.). [Internet] [Masters thesis]. Universidade Federal do Ceará 2013. [cited 2021 Mar 03].
Available from: http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=9438 ;.
Council of Science Editors:
Costa WLG. AnÃlise espacial e temporal da expressÃo de genes relacionados ao metabolismo de lipÃdios em sementes de pinhÃo manso (Jatropha curcas L.). [Masters Thesis]. Universidade Federal do Ceará 2013. Available from: http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=9438 ;
Freie Universität Berlin
5.
Ratert, Nadine.
Referenz-miRNAs für die miRNAome Analyse des Urothelkarzinoms.
Degree: 2014, Freie Universität Berlin
URL: http://dx.doi.org/10.17169/refubium-11948
► Die quantitative reverse Transkriptions-Polymerase-Kettenreaktion (RT-qPCR) ist eine häufig verwendete Methode zur Untersuchung von microRNA(miRNA)-Expressionen bei Tumorerkrankungen. Die relative Quantifizierung der gemessenen miRNAs, basierend auf endogenen…
(more)
▼ Die quantitative reverse Transkriptions-Polymerase-Kettenreaktion (RT-qPCR)
ist eine häufig verwendete Methode zur Untersuchung von
microRNA(miRNA)-Expressionen bei Tumorerkrankungen. Die relative
Quantifizierung der gemessenen miRNAs, basierend auf endogenen Referenzgenen,
ist dabei unerlässlich, um die Variabilität, bedingt durch die einzelnen
Teilschritte der Analyse, zu kompensieren. Eine Literaturrecherche ergab, dass
bis zum jetzigen Zeitpunkt keine Studien zur Ermittlung von Referenz-miRNAs
für das Harnblasenkarzinom vorliegen. Das Ziel dieser Studie war es, in
systematischer Weise geeignete Referenz-miRNAs für RT-qPCR basierte miRNA-
Expressionsstudien des Harnblasenkarzinoms zu identifizieren. Methodik: Unter
Zuhilfenahme eines miRNA-Microarrays wurden aus insgesamt 24 Karzinom- und
Normalgewebeproben der Harnblase Kandidaten von Referenz-miRNAs anhand ihrer
Invarianz in der Expressionsstabilität zwischen den Proben ermittelt. Die
Validierung dieser potenziellen Referenz-miRNAs erfolgte zusammen mit den
häufig in der Literatur verwendeten small RNAs, RNU6B, RNU48 und Z30, an 58
Gewebeproben mittels RTqPCR. Die anschließende bioinformatorische Analyse
wurde mit den Computerprogrammen geNorm, NormFinder und BestKeeper
durchgeführt. Grundlegende Ergebnisse: Insgesamt wurden 16 potenzielle
Referenz-miRNAs auf der Grundlage der miRNAMicroarraydaten identifiziert. Nach
der Validierung mittels RT-qPCR zeigten miR-101, miR-125a-5p, miR-148b,
miR-151-5p, miR-181a, miR-181b, miR-29c, miR-324-3p, miR- 424, miR-874, RNU6B,
RNU48 und Z30 keine Unterschiede zwischen den Gewebeproben, sodass ihre
Eignung als Referenzgene mit den drei Programmen ermittelt werden konnte.
Daraus resultierten unterschiedliche Referenzgenkombinationen.
Schlussfolgerungen: Die vorliegende Studie lieferte die erste systematische
Analyse zur Identifizierung geeigneter Referenz-miRNAs für miRNA-
Expressionsstudien des Harnblasenkarzinoms mittels RT-qPCR. Verschiedene
Referenzgenkombinationen ergaben sowohl für starkals auch für schwach-
regulierte miRNAs vergleichbare Expressionsergebnisse. Besonders eindrucksvoll
konnte die fehlerhafte Normalisierung mit der RNU6B belegt werden, die bisher
am häufigsten in miRNA-Studien als Referenzgen zum Einsatz kam. Die
Kombination aus vier (miR-101, miR-125a-5p, miR-148b und miR-151-5p) bzw. aus
drei (miR-148b, miR-181b und miR-874) Referenz-miRNAs wird für die
Normalisierung von Expressionsstudien beim Harnblasenkarzinom empfohlen.
Advisors/Committee Members: w (gender), N.N. (firstReferee), N.N. (furtherReferee).
Subjects/Keywords: microRNAs; urothelial carcinomas; reference genes; RT-qPCR; 600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit
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APA ·
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APA (6th Edition):
Ratert, N. (2014). Referenz-miRNAs für die miRNAome Analyse des Urothelkarzinoms. (Thesis). Freie Universität Berlin. Retrieved from http://dx.doi.org/10.17169/refubium-11948
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Ratert, Nadine. “Referenz-miRNAs für die miRNAome Analyse des Urothelkarzinoms.” 2014. Thesis, Freie Universität Berlin. Accessed March 03, 2021.
http://dx.doi.org/10.17169/refubium-11948.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Ratert, Nadine. “Referenz-miRNAs für die miRNAome Analyse des Urothelkarzinoms.” 2014. Web. 03 Mar 2021.
Vancouver:
Ratert N. Referenz-miRNAs für die miRNAome Analyse des Urothelkarzinoms. [Internet] [Thesis]. Freie Universität Berlin; 2014. [cited 2021 Mar 03].
Available from: http://dx.doi.org/10.17169/refubium-11948.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Ratert N. Referenz-miRNAs für die miRNAome Analyse des Urothelkarzinoms. [Thesis]. Freie Universität Berlin; 2014. Available from: http://dx.doi.org/10.17169/refubium-11948
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Adelaide
6.
Cook, Naomi Louise.
Characterisation of substance P and transient receptor potential melastatin channel messenger RNA and protein expression in acute and chronic neurological disorders.
Degree: 2010, University of Adelaide
URL: http://hdl.handle.net/2440/64011
► Traumatic brain injury (TBI) is the leading cause of death and disability in people under 40 years of age, with motor vehicle incidents accounting for…
(more)
▼ Traumatic brain injury (TBI) is the leading cause of death and disability in people under 40 years of age, with motor vehicle incidents accounting for the majority of severe TBI cases.
Despite the public health burden of TBI, there are no effective treatment options available, with survivors often left with debilitating long-term deficits. Following TBI, a cascade of
pathophysiological processes is initiated in the central nervous system, including oedema, inflammation, magnesium decline and oxidative stress. These factors play a role in the high
morbidity and mortality following TBI, however, their underlying molecular mechanisms remain poorly understood.
Parkinson’s Disease (PD) is a common neurodegenerative disease and affects approximately 1 % of the population over 65 years of age. PD is characterised by the progressive degeneration of dopaminergic neurons in the substantia nigra pars compacta, leading to a reduction of dopamine levels in the striatum. The pathogenesis of PD is poorly understood, but is likely to involve oxidative stress and inflammatory processes. Current treatments that replace dopamine lose efficacy after several years.
Treatments for TBI and PD are thus urgently required; this requires a greater understanding of the pathophysiology of these disorders at a molecular level. Recent studies from our
laboratory have demonstrated a link between the neuropeptide, substance P (SP), and the development of cerebral oedema and neurologic deficits following TBI, which are attenuated with the administration of an NK-1 (neurokinin-1, SP receptor) antagonist. In addition, studies using a rat model of PD have similarly established a putative role for SP in this disease
process.
Transient receptor potential melastatin (TRPM) channels are a diverse family of ion channels, many of which are highly expressed in the brain. It is likely that TRPM7 and TRPM6 regulate cellular magnesium homeostasis. TRPM7 and TRPM2 are critical mediators of ischaemic neuronal death, and mutations in the TRPM7 and TRPM2
genes confer a genetic susceptibility to parkinsonism. The function of TRPM3 is not well understood, but evidence suggests it may be involved in brain function.
The aims of the present thesis were to: quantify the mRNA level and protein expression of SP, TRPM2, TRPM3, TRPM6 and TRPM7 channels following TBI in human clinical cases and over a time course of experimental TBI in rats; and to characterise the mRNA level of SP, TRPM2, TRPM3 and TRPM7 channels in both clinical PD cases and two rodent models of PD (early and late disease stage), and the protein expression of TRPM channels in early experimental PD.
We demonstrate an upregulation of SP expression in clinical and experimental TBI, supporting our previous studies implicating SP release with TBI pathophysiology. Changes in TRPM channel expression at both the transcript and protein level were also observed following both TBI and in PD, suggesting that TRPM channels may contribute to the oxidative stress, inflammation and neuronal death associated with these…
Advisors/Committee Members: Vink, Robert (advisor), Van Den Heuvel, Corinna (advisor), School of Medical Sciences (school).
Subjects/Keywords: traumatic brain injury; Parkinson's disease; MRNA quantification; real-time RT-PCR; reference genes; neuropeptides; transient receptor potential melastatin channels; immunohisto-chemistry
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APA (6th Edition):
Cook, N. L. (2010). Characterisation of substance P and transient receptor potential melastatin channel messenger RNA and protein expression in acute and chronic neurological disorders. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/64011
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Cook, Naomi Louise. “Characterisation of substance P and transient receptor potential melastatin channel messenger RNA and protein expression in acute and chronic neurological disorders.” 2010. Thesis, University of Adelaide. Accessed March 03, 2021.
http://hdl.handle.net/2440/64011.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Cook, Naomi Louise. “Characterisation of substance P and transient receptor potential melastatin channel messenger RNA and protein expression in acute and chronic neurological disorders.” 2010. Web. 03 Mar 2021.
Vancouver:
Cook NL. Characterisation of substance P and transient receptor potential melastatin channel messenger RNA and protein expression in acute and chronic neurological disorders. [Internet] [Thesis]. University of Adelaide; 2010. [cited 2021 Mar 03].
Available from: http://hdl.handle.net/2440/64011.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Cook NL. Characterisation of substance P and transient receptor potential melastatin channel messenger RNA and protein expression in acute and chronic neurological disorders. [Thesis]. University of Adelaide; 2010. Available from: http://hdl.handle.net/2440/64011
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Freie Universität Berlin
7.
Wierschke, Stephan.
Functional and molecular characterization of hyperpolarization-activated
cation currents in the human epileptogenic neocortex.
Degree: 2011, Freie Universität Berlin
URL: http://dx.doi.org/10.17169/refubium-4313
► The hyperpolarization-activated cation current (I-h) is mediated by the family of HCN channels and plays a pivotal role in the control of neuronal excitability. Studies…
(more)
▼ The hyperpolarization-activated cation current (I-h) is mediated by the family
of HCN channels and plays a pivotal role in the control of neuronal
excitability. Studies on animal models of temporal lobe epilepsy (TLE) have
shown functional deficits of Ih or a reduced expression of HCN-channels.
Possible alterations of I-h in human TLE have not been investigated in detail,
so far. Here, the biophysical properties of Ih were investigated in neurons
(layer II/III) of human epileptogenic neocortex using somatic patch-clamp
recordings (whole-cell mode). The tissues investigated comprise epileptogenic
areas of the neocortex that were resected for therapeutical reasons from
patients with pharmacoresistant TLE or frontal lobe epilepsy (FLE). In some
TLE-tissues (and autopsy controls) the mRNA of the channel subunits HCN1–HCN4
were determined using quantitative PCR and the amount of HCN1 protein was
investigated by immunoblotting. Neurons from TLE tissues and FLE tissues
exhibited (beside a Ba2+-sensitive I-Kir) inward currents with typical
biophysical and pharmacological properties of I-h. Compared to rat neocortical
neurons, the current densities of the fast I-h-component (I-h-fast) were 63 %
smaller than in TLE tissues. Furthermore, the kinetics of activation were
slower and the voltage-dependence was shifted to more negative potentials in
TLE. In FLE tissues the density of I-h-fast was 44 % larger and the current
activation was faster than in TLE tissues. In TLE tissues from patients that
suffered many seizures the mean density of I-h-fast was decreased by ~24 % in
comparison to TLE tissues from patients that suffered many seizures. First
measurements of the temporal summation of artificial EPSP revealed a more
efficient summation in neurons with a small I-h than in neurons with a large
I-h (e. g. in FLE tissues). Compared to autopsy controls, the HCN1 mRNA was
down regulated (by ~50 %) and the HCN4-mRNA was up-regulated (by ~150 %) in
TLE tissues, independent of the seizure frequency. The amount of HCN1 protein
was about 50 % less in TLE tissues from patients with many seizures than in
tissues from patients with few seizures. In conclusion, the data presented
reveal a considerable reduction of cortical I-h in human TLE – primarily in
patients that suffered many seizures. The measurements of the mRNA of the
different subunits suggest that in TLE the number of functional channels
(consisting of HCN1 subunits) is decreased at the transcriptional level.
However, the different amounts of HCN1 protein (present at similar mRNA
levels) between TLE tissues from patients with few seizures and patients with
many seizures might indicate that post-transcriptional mechanisms may play a
role as well. A pathophysiological loss of HCN channels and subsequently of
I-h might increase the network excitability and facilitate the spread of
seizure activity.
Advisors/Committee Members: m (gender), PD Dr. Dr. habil R. A. Deisz (firstReferee), Prof. Dr. H.-J. Pflüger (furtherReferee).
Subjects/Keywords: HCN channels; potassium currents; temporal lobe epilepsy; patch clamp; reference genes; 500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie
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APA (6th Edition):
Wierschke, S. (2011). Functional and molecular characterization of hyperpolarization-activated
cation currents in the human epileptogenic neocortex. (Thesis). Freie Universität Berlin. Retrieved from http://dx.doi.org/10.17169/refubium-4313
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Wierschke, Stephan. “Functional and molecular characterization of hyperpolarization-activated
cation currents in the human epileptogenic neocortex.” 2011. Thesis, Freie Universität Berlin. Accessed March 03, 2021.
http://dx.doi.org/10.17169/refubium-4313.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Wierschke, Stephan. “Functional and molecular characterization of hyperpolarization-activated
cation currents in the human epileptogenic neocortex.” 2011. Web. 03 Mar 2021.
Vancouver:
Wierschke S. Functional and molecular characterization of hyperpolarization-activated
cation currents in the human epileptogenic neocortex. [Internet] [Thesis]. Freie Universität Berlin; 2011. [cited 2021 Mar 03].
Available from: http://dx.doi.org/10.17169/refubium-4313.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Wierschke S. Functional and molecular characterization of hyperpolarization-activated
cation currents in the human epileptogenic neocortex. [Thesis]. Freie Universität Berlin; 2011. Available from: http://dx.doi.org/10.17169/refubium-4313
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
8.
Vossaert, Liesbeth.
Histone H3 clipping in human embryonic stem cells: in pursuit of an epigenetic outcast.
Degree: 2014, Ghent University
URL: http://hdl.handle.net/1854/LU-5782946
► Epigenetic mechanisms such as posttranslational histone modifications (PTM) regulate gene expression (variations) without altering the DNA sequence itself. Human embryonic stem cells (hESC) are a…
(more)
▼ Epigenetic mechanisms such as posttranslational histone modifications (PTM) regulate gene expression (variations) without altering the DNA sequence itself. Human embryonic stem cells (hESC) are a relevant model to study these mechanisms and are hallmarked by self-renewal and pluripotency. Epigenetics plays a key role in regulating the balance between pluripotency and differentiation, and the accompanying developmental processes.
Upon hESC differentiation, the epigenetic signature and thus transcriptional activity, alters drastically. Those gene expression variations can be monitored via RT-qPCR. To correct for technical variability introduced along the experiment, RT-qPCR data can be normalized to several
reference genes selected according to the experimental set-up. As such, B2M, RPL13A and Alu repeats appeared the most stable references for hESC undergoing retinoic acid-induced differentiation and normalization based on this set yielded a significantly different result compared to the use of classic references such as GAPDH.
A whole range of PTM are already described and one particularly interesting is histone clipping, i.e. the removal of a series of amino acids from a histone tail. Based on an earlier publication describing N-terminal histone H3 clipping catalyzed by cathepsin L in differentiating mouse ESC, we showed for the first time that this event also occurs in hESC. However, in this setting H3 clipping occurs in both pluripotent and differentiating hESC, thus a direct link with the differentiation process was not found. We identified three possible cleavage sites and found that the reaction was catalyzed by a serine protease. Histone clipping can severely disturb the epigenetic pattern as it removes existing PTM, disturbs chromatin stability, abolishes the binding of interacting non-histone proteins or conversely creates new target sites. More research is needed to fully uncover the purpose of clipping, but its epigenetic potential is yet obviously clear.
Advisors/Committee Members: Deforce, Dieter, De Sutter, Petra, Dhaenens, Maarten.
Subjects/Keywords: Biology and Life Sciences; Histone modifications; Epigenetics; Human embryonic stem cells; Histone proteolysis; Histone clipping; Proteomics; Quantitative PCR; Reference genes
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APA (6th Edition):
Vossaert, L. (2014). Histone H3 clipping in human embryonic stem cells: in pursuit of an epigenetic outcast. (Thesis). Ghent University. Retrieved from http://hdl.handle.net/1854/LU-5782946
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Vossaert, Liesbeth. “Histone H3 clipping in human embryonic stem cells: in pursuit of an epigenetic outcast.” 2014. Thesis, Ghent University. Accessed March 03, 2021.
http://hdl.handle.net/1854/LU-5782946.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Vossaert, Liesbeth. “Histone H3 clipping in human embryonic stem cells: in pursuit of an epigenetic outcast.” 2014. Web. 03 Mar 2021.
Vancouver:
Vossaert L. Histone H3 clipping in human embryonic stem cells: in pursuit of an epigenetic outcast. [Internet] [Thesis]. Ghent University; 2014. [cited 2021 Mar 03].
Available from: http://hdl.handle.net/1854/LU-5782946.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Vossaert L. Histone H3 clipping in human embryonic stem cells: in pursuit of an epigenetic outcast. [Thesis]. Ghent University; 2014. Available from: http://hdl.handle.net/1854/LU-5782946
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
9.
Puglia, Giuseppe.
Meccanismi fisiologico-molecolari di germinazione in specie spontanee.
Degree: 2014, Università degli Studi di Catania
URL: http://hdl.handle.net/10761/1553
► Seed dormancy provides a mechanism for plants to delay germination until conditions are optimal for survival of the next generation. Knowledge of dormancy release and…
(more)
▼ Seed dormancy provides a mechanism for plants to delay germination until conditions are optimal for survival of the next generation. Knowledge of dormancy release and dormancy induction requirements is important to increase our understanding about the entire process. Furthermore, to assess the association between gene expression patterns and physiological phase in dormancy is a major goal to allow a positive definition of this condition.
In Chrysanthemum coronarium var. concolor and var. discolor, anatomical and germination analyses showed that non-deep physiological dormancy (PD) is the more plausible type of dormancy for both of them. The genetic composition of Chrysanthemum coronarium varieties was characterized using Inter Simple Sequence Repeat (ISSR) PCR together with a DNA-Barcoding approach.
Seed priming treatments were used in a non-dormant species, Leucanthemum vulgare, to induct secondary dormancy at specific temperature range and a long-priming treatment was used for its release. Fluridone and giberellic acid were tested to investigate the metabolic pathway involved in the onset of secondary dormancy and in its maintenance.
Five probable housekeeping genes, useful as reference genes for quantitative PCR (qPCR) analysis in seed tissue, were identified in Chrysanthemum coronarium and in Leucanthemum vulgare, isolated and confirmed with comparative analysis with homologous genes in model species. One radicle-protrusion associated gene, EXP (Expansin), was identified in the two species, isolated and confirmed. Three dormancy-associated genes, DOG1 (Delay Of Germination 1), FLC (Flowering Locus C), HUB2 (Histone Mono-Ubiquitination 2) were identified in Chrysanthemum coronarium and in Leucanthemum vulgare.
Subjects/Keywords: Area 05 - Scienze biologiche; seed dormancy, seed germination, Chrysanthemum coronarium, Leucanthemum vulgare, physiological dormancy, q-PCR reference genes, varietal genotyping
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CSE |
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APA (6th Edition):
Puglia, G. (2014). Meccanismi fisiologico-molecolari di germinazione in specie spontanee. (Thesis). Università degli Studi di Catania. Retrieved from http://hdl.handle.net/10761/1553
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Puglia, Giuseppe. “Meccanismi fisiologico-molecolari di germinazione in specie spontanee.” 2014. Thesis, Università degli Studi di Catania. Accessed March 03, 2021.
http://hdl.handle.net/10761/1553.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Puglia, Giuseppe. “Meccanismi fisiologico-molecolari di germinazione in specie spontanee.” 2014. Web. 03 Mar 2021.
Vancouver:
Puglia G. Meccanismi fisiologico-molecolari di germinazione in specie spontanee. [Internet] [Thesis]. Università degli Studi di Catania; 2014. [cited 2021 Mar 03].
Available from: http://hdl.handle.net/10761/1553.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Puglia G. Meccanismi fisiologico-molecolari di germinazione in specie spontanee. [Thesis]. Università degli Studi di Catania; 2014. Available from: http://hdl.handle.net/10761/1553
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
10.
P. Modesto.
EXPRESSION PROFILE OF IMMUNE RESPONSE GENES IN GOATS WITH EXPERIMENTALLY INDUCED STAPHYLOCOCCUS AUREUS MASTITIS.
Degree: 2012, Università degli Studi di Milano
URL: http://hdl.handle.net/2434/169565
► Counteracting infectious diseases of farm animals are an everlasting challenge in food production from livestock and preserving the health of farm animals is highly relevant…
(more)
▼ Counteracting infectious diseases of farm animals are an everlasting challenge in food production from livestock and preserving the health of farm animals is highly relevant to maintaining high standards of food quality. Clinical mastitis (CM) is the primary health reason for involuntary culling in dairy small ruminants and causes additional economic losses from costs of veterinary treatments. Complementary strategies are needed, since the classical prophylactic measures sometimes appear too demanding to breeders in terms of time and care, and efficient vaccination against the main pathogens is still lacking. There is evidence that Somatic Cell Count (SCC)-based selection should efficiently reduce CM incidence and currently selection strategies in cows and sheeps are based on a linear decrease of milk SCC. The effects and efficacy of SCC selection in goats are still unknown. A better understanding of the defense mechanisms affected and modified by SCC-based selection would be helpful to predict the indirect response for CM, pathogen-specific infections, and resistance to other diseases in the long term. Knowledge of these basic mechanisms will help to the design new and optimized strategies to prevent infections and, at the same time, significantly aid the improvement of food safety for the consumer. Microarray technology enables the examination of complex interactions between the host and bacterial pathogens. In dairy ruminants transcriptome profiling has enabled the identification of
genes, pathways and regulatory networks activated in mammary tissues during experimental infection by various pathogens, including E. coli, S. aureus and S. uberis. Information in goats are still low and many host-pathogen interaction mechanisms have to be explained. In our study the bovine CustomArray 90K was used to evaluate the gene expression in milk somatic cells (MSCs) and blood of goats infected by S. aureus.The objectives of the present study were: (i) to identify the network of
genes that becomes activated in caprine blood and MSCs in early response upon a S. aureus challenge in order to better understand the local and sistemic response and (ii) to search any difference in this immune response by using two animal groups belonging to a caprine
reference family established based on founders with adverse SCC breeding values, (iii) to develop a set of internal
reference genes useful to normalize RT-qPCR data in studies of gene expression in caprine MSCs. A total of 300
genes were found to be differentially expressed between 0 h and 24 h post infection and 128
genes between 0 h and 30 h post infection, with a p value < 0.01 and log2 fold change > 1.5. Among these, the majority were up-regulated. In leukocytes a total of 8
genes were up-regulated between 0h and 30h post infection with a p value < 0.01 and log2 fold change > 1.5 and 1 was down-regulated during IMI. The top up-regulated
genes (5.65 to 3.16 fold change) plays an important role (i) in immune and inflammatory response (NFKB1, TNFAIP6, BASP1, IRF1, PLEK, BATF3); (ii) in…
Advisors/Committee Members: supervisor: V. Bronzo, BRONZO, VALERIO, BRONZO, VALERIO.
Subjects/Keywords: mastitis; microarray; real time qPCR; gene expression; reference genes; goats; Settore VET/05 - Malattie Infettive degli Animali Domestici; Settore BIO/18 - Genetica
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APA ·
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MLA ·
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CSE |
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to Zotero / EndNote / Reference
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APA (6th Edition):
Modesto, P. (2012). EXPRESSION PROFILE OF IMMUNE RESPONSE GENES IN GOATS WITH EXPERIMENTALLY INDUCED STAPHYLOCOCCUS AUREUS MASTITIS. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/169565
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Modesto, P.. “EXPRESSION PROFILE OF IMMUNE RESPONSE GENES IN GOATS WITH EXPERIMENTALLY INDUCED STAPHYLOCOCCUS AUREUS MASTITIS.” 2012. Thesis, Università degli Studi di Milano. Accessed March 03, 2021.
http://hdl.handle.net/2434/169565.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Modesto, P.. “EXPRESSION PROFILE OF IMMUNE RESPONSE GENES IN GOATS WITH EXPERIMENTALLY INDUCED STAPHYLOCOCCUS AUREUS MASTITIS.” 2012. Web. 03 Mar 2021.
Vancouver:
Modesto P. EXPRESSION PROFILE OF IMMUNE RESPONSE GENES IN GOATS WITH EXPERIMENTALLY INDUCED STAPHYLOCOCCUS AUREUS MASTITIS. [Internet] [Thesis]. Università degli Studi di Milano; 2012. [cited 2021 Mar 03].
Available from: http://hdl.handle.net/2434/169565.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Modesto P. EXPRESSION PROFILE OF IMMUNE RESPONSE GENES IN GOATS WITH EXPERIMENTALLY INDUCED STAPHYLOCOCCUS AUREUS MASTITIS. [Thesis]. Università degli Studi di Milano; 2012. Available from: http://hdl.handle.net/2434/169565
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Freie Universität Berlin
11.
Wotschofsky, Zofia.
Deregulated microRNAs and their diagnostic and prognostic impact in clear cell
renal cell carcinoma.
Degree: 2014, Freie Universität Berlin
URL: http://dx.doi.org/10.17169/refubium-13113
► Background/Objective: MicroRNAs (miRNAs) are non-protein coding RNAs of approximately 22 nucleotides and are involved in the regulation of about 30% of all genes. They play…
(more)
▼ Background/Objective: MicroRNAs (miRNAs) are non-protein coding RNAs of
approximately 22 nucleotides and are involved in the regulation of about 30%
of all
genes. They play an important role in cancerogenesis and cancer
progression. Since their significance in clear cell renal cell carcinoma
(ccRCC) was limited at the beginning of my doctoral thesis, it was the aim to
evaluate the diagnostic and prognostic potential of miRNAs in ccRCC. Methods:
Microarray analyses of miRNAs from normal and cancerous samples of ccRCC
tissue collected after radical nephrectomy and from bone metastases of ccRCC
patients were performed to identify both invariant miRNAs as potential
referencemiRNAs for relative quantification and differentially expressed
miRNAs as diagnostic and prognostic indicators. The validation studies were
performed by quantitative reverse transcription polymerase chain reaction (RT-
qPCR) analyses. Different special software (geNorm, NormFinder, prediction
tools) and standard statistical programs were used for calculations. Results:
In the first study, miR-28, miR-103 and miR-106a were proved as the most
stably expressed miRNAs in the different tissue samples. Consequently, the
combinations of miR-28, miR-103, and miR-106a or miR-28 and miR-103 were
recommended as preferred normalizer approaches for relative quantification.
MiR-28 could be used as single normalizer in case of shortage of sample
material while RNU6B that is frequently used was unsuitable as normalizer. In
the second study, 30 miRNAs were identified to be particularly deregulated
between tumor and normal tissue samples. A stepwise down-regulation of miRNA
expression from normal over primary tumor to metastatic tissue was typical
while only six miRNAs were up-regulated in metastatic tissue in comparison to
normal tissue. Seventeen miRNAs were detected as novel miRNAs associated with
ccRCC metastasis that were not recognized as such in previous studies. Based
on these and previous findings, four up-regulated and four down-regulated
miRNAs in malignant and non-malignant samples after nephrectomy from patients
without (n=89) and with (n=22) metastases were measured. All miRNAs were found
to be suitable indicators to differentiate malignant from non-malignant
tissue. MiR-122 and miR-514 were significantly related to the recurrence risk
after nephrectomy and miR-514 was an independent prognostic variable in a
final Coxregression model together with clinicopathological variables.
Conclusions: Based on these studies, it could be shown that miRNA expression
data not only results in promising diagnostic and prognostic information in
completion to conventional clinicopathological data. They also provide novel
insights in yet unknown molecular processes of cancer progression and offer
new therapeutic strategies.
Advisors/Committee Members: w (gender), N.N. (firstReferee), N.N. (furtherReferee).
Subjects/Keywords: clear cell renal cell carcinoma; microRNAs; reference genes; RT-qPCR; Metastasis; recurrence risk prediction; prognostic markers; 600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit
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APA ·
Chicago ·
MLA ·
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CSE |
Export
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Manager
APA (6th Edition):
Wotschofsky, Z. (2014). Deregulated microRNAs and their diagnostic and prognostic impact in clear cell
renal cell carcinoma. (Thesis). Freie Universität Berlin. Retrieved from http://dx.doi.org/10.17169/refubium-13113
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Wotschofsky, Zofia. “Deregulated microRNAs and their diagnostic and prognostic impact in clear cell
renal cell carcinoma.” 2014. Thesis, Freie Universität Berlin. Accessed March 03, 2021.
http://dx.doi.org/10.17169/refubium-13113.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Wotschofsky, Zofia. “Deregulated microRNAs and their diagnostic and prognostic impact in clear cell
renal cell carcinoma.” 2014. Web. 03 Mar 2021.
Vancouver:
Wotschofsky Z. Deregulated microRNAs and their diagnostic and prognostic impact in clear cell
renal cell carcinoma. [Internet] [Thesis]. Freie Universität Berlin; 2014. [cited 2021 Mar 03].
Available from: http://dx.doi.org/10.17169/refubium-13113.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Wotschofsky Z. Deregulated microRNAs and their diagnostic and prognostic impact in clear cell
renal cell carcinoma. [Thesis]. Freie Universität Berlin; 2014. Available from: http://dx.doi.org/10.17169/refubium-13113
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
12.
Isana Mara AragÃo Frota.
Estabilidade de genes de referÃncia e influÃncia das BMPs-6 e 7 sobre o desenvolvimento in vitro de folÃculos prÃ-antrais caprinos.
Degree: Master, 2010, Universidade Federal do Ceará
URL: http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=4680
;
► Este trabalho tem como objetivo avaliar a estabilidade de genes de referÃncia e a expressÃo de fatores de crescimento e de receptores de hormÃnios em…
(more)
▼ Este trabalho tem como objetivo avaliar a estabilidade de genes de referÃncia e a expressÃo de fatores de crescimento e de receptores de hormÃnios em folÃculos secundÃrios de caprinos. AlÃm disso, visa quantificar a expressÃo dos RNA mensageiros para BMP-6 e BMP-7 em folÃculos prÃ-antrais e antrais caprinos e avaliar os efeitos de FSH, BMP-6 e BMP-7 sobre o crescimento e expressÃo gÃnica em folÃculos secundÃrios caprinos durante 6 dias de cultivo. Para avaliar a estabilidade dos genes de referÃncia, folÃculos secundÃrios (150-200 Âm) foram isolados mecanicamente de ovÃrios caprinos. ApÃs a extraÃÃo do RNA total e sÃntese de DNA complementar, realizou a quantificaÃÃo dos mRNA, por PCR em tempo real, utilizando-se primers especÃficos para genes de referÃncia (GAPDH, β-tubulina, β-actina, PGK, UBQ, RPL-19, rRNA18S), fatores de crescimento (GDF-9, BMP-15, BMP-6, FGF-2, VEGF, KL e IGF-1) e receptores de hormÃnio (FSH-R, LH-R e GH-R). Para avaliar a expressÃo de BMP-6 e BMP-7, folÃculos primordiais, primÃrios e secundÃrios, bem como pequenos e grandes folÃculos antrais foram obtidos e os nÃveis de mRNA de BMP-6 e 7 foram quantificados. Nos estudos in vitro, os efeitos da BMP-6 (50 ng/mL) e BMP-7 (50 ng/mL) na presenÃa ou ausÃncia de FSH (50 ng/mL) sobre o desenvolvimento in vitro de folÃculos secundÃrios e sobre a expressÃo de mRNA para BMP-6 e 7 e FSH-R foi avaliada apÃs 6 dias de cultivo. Os resultados mostraram que UBQ e β-actina sÃo os genes de referÃncia mais estÃveis em folÃculos prÃ-antrais caprinos fresco cultivados por 12 dias. Os RNAs mensageiros para os fatores de crescimento (EGF, GDF-9, BMP-15, VEGF, FGF-2, BMP-6, IGF-1 e KL) e os receptores de FSH, LH e GH sÃo expressos em diferentes nÃveis em folÃculos prÃ-antrais de caprinos, sendo que o IGF-1 e o EGF apresentaram, respectivamente, o maior e o menor nÃvel de mRNA. O nÃvel de mRNA para BMP-6 em folÃculos primÃrios e secundÃrios foi significativamente maior do que aqueles em primordial, enquanto que os nÃveis de mRNA para BMP-7 foi maior nas cÃlulas da granulosa/teca de grandes do que nos pequenos folÃculos antrais. ApÃs o cultivo de folÃculos secundÃrios durante 6 dias, FSH aumentou o diÃmetro folicular e FSH e BMP-7 aumentou significativamente os nÃveis de mRNA para BMP-7 e FSH-R. JÃ a BMP-6 na presenÃa ou ausÃncia de FSH aumentou o diÃmetro dos folÃculos secundÃrios. AlÃm disso, FSH aumentou os nÃveis de mRNA para BMP-6, enquanto ambos BMP-6 e FSH e aumentaram os nÃveis de mRNA para FSH-R, apÃs o perÃodo de cultivo. Em conclusÃo, UBQ e β-actina sÃo os dois genes mais estÃveis para folÃculos secundÃrios caprinos e o FSH e as BMPs dos tipos 6 e 7 estimulam o crescimento de folÃculos prÃ-antrais in vitro durante 6 dias de cultivo.
This study aims to evaluate the stability of reference genes and the expression of growth
factors and hormone receptors in goat secondary follicles. It also seeks to quantify the
expression of messenger RNA for BMP-6 and BMP-7 in goat preantral follicles and to
evaluate the effects of FSH, BMP-6 and BMP-7 on…
Advisors/Committee Members: FabrÃcio de Sousa Martins, Alice Andrioli Pinheiro, Josà Roberto Viana Silva.
Subjects/Keywords: CiÃncias BiolÃgicas; BMP-6; BMP-7; Receptores; Genes de ReferÃncia; FolÃculos PrÃ-antrais; BMP-6; BMP-7; Hormone Receptors; Genes of Reference; Preantral Follicle; Engenharia GenÃtica;
Caprinos - ReproduÃÃo;
Caprinos - GenÃtica;
FolÃculo Ovariano - fisiologia;
OvÃrio - fisiologia; RNA Mensageiro;
ReproduÃÃo;
Fertilidade; Biotecnologia
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APA (6th Edition):
Frota, I. M. A. (2010). Estabilidade de genes de referÃncia e influÃncia das BMPs-6 e 7 sobre o desenvolvimento in vitro de folÃculos prÃ-antrais caprinos. (Masters Thesis). Universidade Federal do Ceará. Retrieved from http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=4680 ;
Chicago Manual of Style (16th Edition):
Frota, Isana Mara AragÃo. “Estabilidade de genes de referÃncia e influÃncia das BMPs-6 e 7 sobre o desenvolvimento in vitro de folÃculos prÃ-antrais caprinos.” 2010. Masters Thesis, Universidade Federal do Ceará. Accessed March 03, 2021.
http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=4680 ;.
MLA Handbook (7th Edition):
Frota, Isana Mara AragÃo. “Estabilidade de genes de referÃncia e influÃncia das BMPs-6 e 7 sobre o desenvolvimento in vitro de folÃculos prÃ-antrais caprinos.” 2010. Web. 03 Mar 2021.
Vancouver:
Frota IMA. Estabilidade de genes de referÃncia e influÃncia das BMPs-6 e 7 sobre o desenvolvimento in vitro de folÃculos prÃ-antrais caprinos. [Internet] [Masters thesis]. Universidade Federal do Ceará 2010. [cited 2021 Mar 03].
Available from: http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=4680 ;.
Council of Science Editors:
Frota IMA. Estabilidade de genes de referÃncia e influÃncia das BMPs-6 e 7 sobre o desenvolvimento in vitro de folÃculos prÃ-antrais caprinos. [Masters Thesis]. Universidade Federal do Ceará 2010. Available from: http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=4680 ;
13.
Hagos, Daniel Yemane.
Analysis of the expression of INSR and FOX Genes in Celiac Disease.
Degree: Life Sciences, 2012, University of Skövde
URL: http://urn.kb.se/resolve?urn=urn:nbn:se:his:diva-6847
► Celiac disease (CD) is a common heritable immune related disorder where chronic inflammationof the small intestine is induced by the ingestion of gluten. The…
(more)
▼ Celiac disease (CD) is a common heritable immune related disorder where chronic inflammationof the small intestine is induced by the ingestion of gluten. The immune response leads to theinflammation and flattening of intestinal mucosa due to the damaged villi and thus results indefects in the absorption of nutrients. This defect can affect any organ or body system and exposeto the risk of lifelong complications such as cancer, autoimmune diseases and other complexdiseases. Now a day, celiac disease is becoming one of the well-studied models of complexdisorders.The PI3K- FOX signaling pathway is activated by many regulators and growth factors and playsa key role in cell cycle. Two components of this pathway, INSR and FOX, play crucial roles indiverse aspects of embryogenesis from the initial tissue genesis up to organ formation. INSR andFOX take part in development, differentiation, proliferation, apoptosis and stress resistance aswell as metabolism. SNP´s could affect the expression of neighboring genes. These SNP´s areshown to be as eQTLs, genomic loci that regulate the expression of genes. The aim of this studywas to detect and quantitate the expression of INSR and certain FOX genes in celiac disease.Quantitative real time PCR (QPCR) was used to analyze the expression of INSR, FOXO1,FOXO4 and FOXD3 genes in 38 celiac cases and 50 control samples. Three reference genesACTB, EPCAM and PGK1 were tested for their expression stability and their average was used inthe normalization procedure. Gene expression results were analyzed using the ΔCt method. Theexpression of INSR, FOXO1, FOXO4 and FOXD3 were described as their fold change in CDcompared to normal non-celiac mucosa. Our results indicated that FOXO4 and INSR wereexpressed less by 0.60 fold and FOXO1 was expressed less by 0.23 fold in CD samples. Theresults are preliminary and further studies will be needed to confirm if these findings are a resultof the intestinal inflammation in CD or if these genes are partly driving the disease itself.
Subjects/Keywords: Celiac disease; reverse transcription; target genes; reference genes; QPCR; Relative quantification; Fold change.
…reference genes were
evaluated at four concentrations (10ng/µl, 2ng/µl, 0.4ng/µl, and 0.08ng… …expression were set to 40 (Ct=40).
•The reference genes were validated by plotting the Ct… …value of the reference genes against
each other so that it could calculate as the geometric… …mean of the three reference genes
(gNorm V < 0.15).
GeNorm module of qbasePLUS v2.1… …x28;Biogazelle, Belgium) was used to determine the most
stable reference genes (…
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APA (6th Edition):
Hagos, D. Y. (2012). Analysis of the expression of INSR and FOX Genes in Celiac Disease. (Thesis). University of Skövde. Retrieved from http://urn.kb.se/resolve?urn=urn:nbn:se:his:diva-6847
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Hagos, Daniel Yemane. “Analysis of the expression of INSR and FOX Genes in Celiac Disease.” 2012. Thesis, University of Skövde. Accessed March 03, 2021.
http://urn.kb.se/resolve?urn=urn:nbn:se:his:diva-6847.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Hagos, Daniel Yemane. “Analysis of the expression of INSR and FOX Genes in Celiac Disease.” 2012. Web. 03 Mar 2021.
Vancouver:
Hagos DY. Analysis of the expression of INSR and FOX Genes in Celiac Disease. [Internet] [Thesis]. University of Skövde; 2012. [cited 2021 Mar 03].
Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:his:diva-6847.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Hagos DY. Analysis of the expression of INSR and FOX Genes in Celiac Disease. [Thesis]. University of Skövde; 2012. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:his:diva-6847
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Vienna
14.
Meyer, Florian Rudolf Lukas.
Meta analysis for the finding of tissue specific reference genes in the sections of the small intestine in mice.
Degree: 2010, University of Vienna
URL: http://othes.univie.ac.at/11315/
► Selbst verhältnismäßig kleine Veränderungen der Genexpression können signifkante biologische Effekte haben. Um solche kleinen Veränderungen quantifizieren zu können, muß der Goldstandard für Expressionsquantifzierung, die Reverse…
(more)
▼ Selbst verhältnismäßig kleine Veränderungen der Genexpression können signifkante biologische Effekte haben. Um solche kleinen Veränderungen quantifizieren zu können, muß der Goldstandard für Expressionsquantifzierung, die Reverse Transcription Quantitative Real-Time PCR (RT-qPCR), mit einer passenden Normalisierungsstrategie verbunden werden. Der erfolgreiche Einsatz dieser Technik benötigt eine sorgfältige Auswahl von Referenzgenen für die Normalisierung. Gewebeübergreifende Expressionsanalysen benötigen Gene die in allen Geweben gleichmäßig stabil exprimiert werden, wodurch nur eine geringe Anzahl an einigermaßen stabilen Genen zur Verfügung steht. Wenn jedoch nur ein bestimmtes Gewebe untersucht wird, dürfte die Anzahl der möglichen Referenzgene jedoch steigen. In dieser Arbeit wurde nach Referenzgenen gesucht, die speziell auf die einzelnen Abschnitte des Dünndarms von Mäusen abgestimmt sind. Kandidatengene wurden durch eine Metaanalyse ermittelt. Für die Metaanalyse wurden Daten von internen und öffentlich zugänglichen Microarrays verwendet.
Jejunum Proben eines Auszuchtstammes und drei Inzuchtstämmen wurden verwendet um
15 Kandidatengene mittles RT-qPCR zu validieren. Die Gene Plekha7, 6430706D22Rik, EG666853 und Zfyve19 wurden als diejenigen identifiziert die als interne Kontrollen am geeignetsten sind, da ihr Expression nur um <0.8-fach schwankten. Ihre Expressionstabilität liegt im selben Bereich wie jene von Oaz1, übertrifft jedoch die der kürzlich für gewebeübergreifene Normalisierung eingeführten Retrotransposons B1 Element und B2 Element. Der Expressions Stabilitätswert, errechnet durch die Software GeNorm, übertrifft die Werte früherer gewebe- und plattformübergreifenden Metaanalysen.
Die hohe Stabilität der neu gefunden Jejunum-Referenzgene legt nahe, daß sie in diesem Gewebe, in stark regulierten Stoffwechselwegen involviert sind. Die funktionelle Diversivität der neu gefundenen Referenzgene erlaubt es Zielgene unter einer Vielzahl biologischer Konditionen und Stimuli zu untersuchen. Zusätzlich wurde dieser Metaanalyseansatz noch auf die anderen Untereinheiten des murinen Dünndarms und auf den gesamten Dünndarm angewendet.
Die Verbesserung der Genauigkeit der Normalisierung durch eine Metaanalyse von Expressionsdaten eines spezifischen Gewebes unterstreicht die Möglichkeiten dieser Strategie in anderen Geweben. Durch ihre, impliziert durch ihre gleichförmige Expression, biologische Bedeutung sind die neu gefundenen Normalisierungsgene auch lohnende Ziele für weitere Untersuchungen.
Even relatively modest expression changes can have significant biological effects. To quantify such low expression changes the gold standard reverse transcription quantitative real-time PCR (RT-qPCR) has to be combined with accurate normalisation. Successful application of this technique relies on the careful selection of reference genes for normalisation. Tissue wide expression profiling necessitates reference genes similarly stably expressed in every tissue resulting in only a small proportion of genes with a…
Subjects/Keywords: 42.30 Mikrobiologie; 42.64 Tiergenetik; 42.20 Genetik; 42.03 Methoden und Techniken der Biologie; Metaanalyse / Dünndarm / Maus / Duodenum / Jejunum / Ileum / Referenzgene / Normalisierung / Eva-Green / qRT-PCR / Plekha7 / 6430706D22Rik / EG666853 / Zfyve19; metaanalysis / small intestine / mouse / duodenum / jejunum / ileum / reference genes / normalisation / Eva-Green / qRT-PCR / Plekha7 / 6430706D22Rik / EG666853 / Zfyve19
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APA ·
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APA (6th Edition):
Meyer, F. R. L. (2010). Meta analysis for the finding of tissue specific reference genes in the sections of the small intestine in mice. (Thesis). University of Vienna. Retrieved from http://othes.univie.ac.at/11315/
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Meyer, Florian Rudolf Lukas. “Meta analysis for the finding of tissue specific reference genes in the sections of the small intestine in mice.” 2010. Thesis, University of Vienna. Accessed March 03, 2021.
http://othes.univie.ac.at/11315/.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Meyer, Florian Rudolf Lukas. “Meta analysis for the finding of tissue specific reference genes in the sections of the small intestine in mice.” 2010. Web. 03 Mar 2021.
Vancouver:
Meyer FRL. Meta analysis for the finding of tissue specific reference genes in the sections of the small intestine in mice. [Internet] [Thesis]. University of Vienna; 2010. [cited 2021 Mar 03].
Available from: http://othes.univie.ac.at/11315/.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Meyer FRL. Meta analysis for the finding of tissue specific reference genes in the sections of the small intestine in mice. [Thesis]. University of Vienna; 2010. Available from: http://othes.univie.ac.at/11315/
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Université de Montréal
15.
Nachar, Walid.
Sélection de gènes de référence pour la normalisation des expériences de PCR quantitative dans un modèle de dysfonction diastolique de lapin.
Degree: 2014, Université de Montréal
URL: http://hdl.handle.net/1866/10861
Subjects/Keywords: Dysfonction diastolique; Lapin; RT-qPCR; Gènes de référence; Bnp; Diastolic dysfunction; Rabbit; RT-qPCR; Reference genes; Bnp; Biology - Molecular / Biologie - Biologie moléculaire (UMI : 0307)
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APA (6th Edition):
Nachar, W. (2014). Sélection de gènes de référence pour la normalisation des expériences de PCR quantitative dans un modèle de dysfonction diastolique de lapin. (Thesis). Université de Montréal. Retrieved from http://hdl.handle.net/1866/10861
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Nachar, Walid. “Sélection de gènes de référence pour la normalisation des expériences de PCR quantitative dans un modèle de dysfonction diastolique de lapin.” 2014. Thesis, Université de Montréal. Accessed March 03, 2021.
http://hdl.handle.net/1866/10861.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Nachar, Walid. “Sélection de gènes de référence pour la normalisation des expériences de PCR quantitative dans un modèle de dysfonction diastolique de lapin.” 2014. Web. 03 Mar 2021.
Vancouver:
Nachar W. Sélection de gènes de référence pour la normalisation des expériences de PCR quantitative dans un modèle de dysfonction diastolique de lapin. [Internet] [Thesis]. Université de Montréal; 2014. [cited 2021 Mar 03].
Available from: http://hdl.handle.net/1866/10861.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Nachar W. Sélection de gènes de référence pour la normalisation des expériences de PCR quantitative dans un modèle de dysfonction diastolique de lapin. [Thesis]. Université de Montréal; 2014. Available from: http://hdl.handle.net/1866/10861
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Gothenburg / Göteborgs Universitet
16.
Carlström, Maria.
Effects of Biomechanical Stress on Gene Regulation in Vascular Cells.
Degree: 2007, University of Gothenburg / Göteborgs Universitet
URL: http://hdl.handle.net/2077/7506
► The vascular vessel wall is constantly exposed to biomechanical forces, such as shear and tensile stress. Biomechanical forces are important for several physiological and pathological…
(more)
▼ The vascular vessel wall is constantly exposed to biomechanical forces, such as shear and tensile stress. Biomechanical forces are important for several physiological and pathological processes and have been shown to regulate a number of fundamental vascular functions, such as vascular tone and remodeling processes. The aim of the present thesis was to study the effects of biomechanical forces on the vessel wall.
Intact human conduit vessels were exposed to normal or high intraluminal pressure, or low or high shear stress in combination with a physiological level of the other factor in a unique vascular ex vivo perfusion model, developed in our laboratory. Global gene expression profiling was performed with microarray technology of endothelial cells from stimulated vessels. Biomechanical forces were found to regulate a large number of genes. The fraction of genes that responded to both pressure and shear stimulation was surprisingly low, which indicates that the two different stimuli induce distinct gene expression response patterns. Further, these results suggest that the endothelium has the capacity to discriminate between shear stress and pressure stimulation.
Detection and quantification of changes in gene expression require valid and reliable endogenous references genes. Therefore, the appropriateness of ten reference genes for studies of biomechanically stimulated endothelium was evaluated by microarray technology and real-time RT-PCR.
Shear stress plays an essential role in regulation of vascular tone and remodeling, and P2 receptors have been suggested to be mediators of some of these effects. We therefore studied the effects of shear stress on P2 receptor expression in intact human vessels. In the endothelium, no significant regulation of P2 receptor mRNA levels was observed. However, in smooth muscle cells, high shear stress decreased mRNA expression of the contractile P2X1 receptor and increased the mitogenic P2Y2 and P2Y6 receptors. These findings were consistent at the protein level with Western blot analysis and morphologically with immunohistochemistry. This suggests that the shear force can be transmitted to the underlying smooth muscle cells.
The interplay of shear stress and inflammatory stress on urokinase-type plasminogen activator (u-PA) and plasminogen activator inhibitor-1 (PAI-1) expression was studied in an in vitro shear stress system. Endothelial cells were exposed to either shear stress, the proinflammatory cytokine tumor necrosis factor-α (TNF-a), or a combination of both. High shear stress markedly reduced u-PA expression whereas TNF-a induced u-PA expression. Combining shear stress and inflammatory stimulation reduced the TNF-a mediated u-PA induction, which suggests that shear stress exerts a strong protective effect. The TNF-a induced expression was proposed to be partly mediated by activation of c-jun N-terminal kinase (JNK). The PAI-1 expression was induced both by shear stress and TNF-a, and the effect was potentiated when the two stimuli were combined.
In conclusion, these…
Subjects/Keywords: shear stress; intraluminal pressure; endothelium; gene expression; DNA microarray; real-time RT-PCR; reference genes; smooth muscle cells; P2 receptors; TNF-alpha; urokinase-type plasminogen activator
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APA ·
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CSE |
Export
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Manager
APA (6th Edition):
Carlström, M. (2007). Effects of Biomechanical Stress on Gene Regulation in Vascular Cells. (Thesis). University of Gothenburg / Göteborgs Universitet. Retrieved from http://hdl.handle.net/2077/7506
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Carlström, Maria. “Effects of Biomechanical Stress on Gene Regulation in Vascular Cells.” 2007. Thesis, University of Gothenburg / Göteborgs Universitet. Accessed March 03, 2021.
http://hdl.handle.net/2077/7506.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Carlström, Maria. “Effects of Biomechanical Stress on Gene Regulation in Vascular Cells.” 2007. Web. 03 Mar 2021.
Vancouver:
Carlström M. Effects of Biomechanical Stress on Gene Regulation in Vascular Cells. [Internet] [Thesis]. University of Gothenburg / Göteborgs Universitet; 2007. [cited 2021 Mar 03].
Available from: http://hdl.handle.net/2077/7506.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Carlström M. Effects of Biomechanical Stress on Gene Regulation in Vascular Cells. [Thesis]. University of Gothenburg / Göteborgs Universitet; 2007. Available from: http://hdl.handle.net/2077/7506
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
17.
Khan, Muhammad.
Effect of Maternal Age on Transcriptome of Granulosa Cells from Bovine Dominant Follicles.
Degree: 2014, University of Saskatchewan
URL: http://hdl.handle.net/10388/ETD-2014-01-1364
► Advanced maternal age has been shown to influence follicular and luteal dynamics in bovine ovary resulting in reduced fertility. The overall objective of the four…
(more)
▼ Advanced maternal age has been shown to influence follicular and luteal dynamics in bovine ovary resulting in reduced fertility. The overall objective of the four studies presented in this thesis is to identify the maternal age-associated transcriptional changes in granulosa cells of the dominant follicles during follicle development.
In the first study, mRNA expression levels of housekeeping
genes were measured by real–time quantitative PCR (RT-qPCR) in granulosa cells of dominant follicles and FSH-stimulated follicles to select and validate suitable
reference genes for relative gene expression analyses during maternal and follicular aging. Stability of six
reference genes (GAPDH, ACTB, EIF2B2, UBE2D2, SF3A1 and RNF20) was analyzed using GeNorm, DeltaCT and NormFinder programs and comprehensive ranking order was determined based on these programs. Geometric mean of multiple
genes (UBE2D2, EIF2B2, GAPDH and SF3A1) was more appropriate
reference control than individual
genes for the comparison of relative gene expression among dominant and FSH-stimulated follicles during maternal and/or follicular aging studies.
In the second study, maternal age-associated changes in the transcriptome of granulosa cells recovered at the time of selection of the dominant follicle from aged (n=3) and young cows (n=3) were determined by EmbryoGENE bovine oligo-microarrays (EMBV3, Agilent Technology). The mRNA expression of five transcripts (CYP19A1, PCNA, GJA1, TPM2, and VNN1) was confirmed in a different set of granulosa cell samples by RT-qPCR to validate microarray data. A total of 169
genes/isoforms were differentially expressed (≥ 2-fold-change; P ≤ 0.05) in aged cows vs. young cows. These transcripts revealed inefficient 1) control of gonadotropins, and gonadotropin-induced changes in the cytoskeleton and extracellular matrix, 2) lipid metabolism and steroidogenesis 3) cell proliferation, cell cycle control and intercellular communication, and 4) higher oxidative stress responses in aged cows vs. young cows.
In the third study, changes in the transcriptome of granulosa cells of the preovulatory follicle 24 h after LH treatment from aged (n= 3) and young (n=3) were determined. A total of 1340
genes were expressed differentially (≥ 2-fold change; P ≤ 0.05) in aged cows vs. young cows. The mRNA expression of five transcripts (RGS2, PTGS2, TNFAIP6, VNN1, NR5A2 and GADD45B) was confirmed in a different set of granulosa cell samples to validate microarray data. These transcripts were related to delayed 1) response to LH treatment 2) cellular differentiation and luteinization and 3) progesterone synthesis. Intra-follicle levels of progesterone were lower (P < 0.05) in aged cows compared to young and mid-aged cows.
The fourth study compared the aged-associated changes in the transcriptome of granulosa cells during follicle development from the time of dominant follicle selection to preovulatory stage (24 h after LH). In comparison to young cows, aged cows expressed fewer differentially expressed
genes/isoforms (1206 vs. 2260, respectively)…
Advisors/Committee Members: Singh, Jaswant, Adams, Gregg P., Sirard, Marc A., Misra, Vikram, Anzar, Muhammad.
Subjects/Keywords: Maternal age; Granulosa cells; Dominant follicle at selection; Preovulatory follicle; Reference genes; Microarrays; RT-qPCR; Bovine; Luteinizing hormone; Ovariectomy; Follicular aspiration; Transcriptome analysis; Ingenuity Pathway Analysis; Upstream regulators; Estradiol; Progesterone; Follicular fluid; Ultrasonography; Ovulation
…Selection of reference genes and primer design… …57
Messenger RNA levels of reference genes… …57
Stability of reference genes… …58
Comprehensive ranking order of reference genes… …59
Optimal number of reference genes…
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APA ·
Chicago ·
MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Khan, M. (2014). Effect of Maternal Age on Transcriptome of Granulosa Cells from Bovine Dominant Follicles. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/ETD-2014-01-1364
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Khan, Muhammad. “Effect of Maternal Age on Transcriptome of Granulosa Cells from Bovine Dominant Follicles.” 2014. Thesis, University of Saskatchewan. Accessed March 03, 2021.
http://hdl.handle.net/10388/ETD-2014-01-1364.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Khan, Muhammad. “Effect of Maternal Age on Transcriptome of Granulosa Cells from Bovine Dominant Follicles.” 2014. Web. 03 Mar 2021.
Vancouver:
Khan M. Effect of Maternal Age on Transcriptome of Granulosa Cells from Bovine Dominant Follicles. [Internet] [Thesis]. University of Saskatchewan; 2014. [cited 2021 Mar 03].
Available from: http://hdl.handle.net/10388/ETD-2014-01-1364.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Khan M. Effect of Maternal Age on Transcriptome of Granulosa Cells from Bovine Dominant Follicles. [Thesis]. University of Saskatchewan; 2014. Available from: http://hdl.handle.net/10388/ETD-2014-01-1364
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
. 
Open Access Theses and Dissertations
