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University of North Carolina – Greensboro
1.
Singh, Jagjeet.
A model system for understanding cellular signaling of the
cannabinoid CB2 receptor via the inhibitory Gi protein.
Degree: 2014, University of North Carolina – Greensboro
URL: http://libres.uncg.edu/ir/listing.aspx?styp=ti&id=16606
► One key signaling pathway in the cellular signaling involving G protein coupled receptors (GPCR) is via heterotrimeric G proteins. The first step in GPCR/G protein…
(more)
▼ One key signaling pathway in the cellular signaling
involving G protein coupled
receptors (GPCR) is via heterotrimeric
G proteins. The first step in GPCR/G protein signaling is the
activation of a GPCR by the ligand binding and the next step is the
activation of the G protein. Understanding the molecular mechanism
behind the GPCR/G protein interactions will help in characterizing
this important signaling pathway and should ultimately lead to the
design of functionally selective ligands for this larger class of
receptors. Earlier, the Reggio group used molecular dynamics
simulations to study the activation of the cannabinoid CB2
receptor, a class A GPCR, by its endogenous ligand,
2-arachidonoylglycerol (2-AG) via the lipid bilayer. The goal of
the current project was to study the next step in the G-protein
mediated signal transduction, when an agonist activated CB2
receptor forms a complex with Gi protein and catalyzes the
activation of Gi protein, releasing the guanosine diphosphate (GDP)
bound between the ras-like (also known as GTPase domain) and
helical domains of the Gá protein. To this end, we report here the
CB2 / Gái1â1ã2 complex formation using our 2-AG activated CB2
receptor model. For G protein activation (dissociation of GDP), we
hypothesized that GDP release from the ras-like and helical domains
of Gái would be triggered by the hydration of GDP. We probed the
role of the CB2 receptor interactions with the Gái protein and the
resultant progression of GDP hydration. We have seen the number of
waters surrounding GDP increase from 16 (t= 0 ns) to 28 waters (t=5
ìs). Two important interactions between the receptor and G-protein
appear to lead to the increased hydration of GDP. (1) A hydrophobic
interaction occurred between CB2 IC2 loop residue P139 and the Gái
hydrophobic pocket residues: V34 (N terminus; L194 (â1 sheet); F196
(â2 sheet); and, F336, T340, I343, I344 (á5 helix) multiple times
in our 5 ìs long trajectory. Each time this interaction occurred,
an increase in GDP hydration was observed in our simulation. 2) We
also observed an IC3 loop interaction with the Gái á4 helix between
1.4 to 1.6 ìs, in which the IC3 loop residue R229 reached to
interact with E297 and E298. Taken together, our results show that
the intracellular loops play a critical role in the hydration of
GDP that should lead to G protein activation.; Cellular signaling,
Cannabinoid CB2 receptor
Advisors/Committee Members: Patricia Reggio (advisor).
Subjects/Keywords: G proteins – Receptors; Cannabinoids – Receptors
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APA (6th Edition):
Singh, J. (2014). A model system for understanding cellular signaling of the
cannabinoid CB2 receptor via the inhibitory Gi protein. (Doctoral Dissertation). University of North Carolina – Greensboro. Retrieved from http://libres.uncg.edu/ir/listing.aspx?styp=ti&id=16606
Chicago Manual of Style (16th Edition):
Singh, Jagjeet. “A model system for understanding cellular signaling of the
cannabinoid CB2 receptor via the inhibitory Gi protein.” 2014. Doctoral Dissertation, University of North Carolina – Greensboro. Accessed March 02, 2021.
http://libres.uncg.edu/ir/listing.aspx?styp=ti&id=16606.
MLA Handbook (7th Edition):
Singh, Jagjeet. “A model system for understanding cellular signaling of the
cannabinoid CB2 receptor via the inhibitory Gi protein.” 2014. Web. 02 Mar 2021.
Vancouver:
Singh J. A model system for understanding cellular signaling of the
cannabinoid CB2 receptor via the inhibitory Gi protein. [Internet] [Doctoral dissertation]. University of North Carolina – Greensboro; 2014. [cited 2021 Mar 02].
Available from: http://libres.uncg.edu/ir/listing.aspx?styp=ti&id=16606.
Council of Science Editors:
Singh J. A model system for understanding cellular signaling of the
cannabinoid CB2 receptor via the inhibitory Gi protein. [Doctoral Dissertation]. University of North Carolina – Greensboro; 2014. Available from: http://libres.uncg.edu/ir/listing.aspx?styp=ti&id=16606

University of Waterloo
2.
Sethi, Neha.
The interaction of chemicals isolated from municipal wastewater effluent with rainbow trout (Oncorhynchus mykiss) thyroid hormone receptors.
Degree: 2012, University of Waterloo
URL: http://hdl.handle.net/10012/7126
► The normal function of the thyroid hormone (TH) system is essential for growth, development and metabolism in humans as well as in other species. The…
(more)
▼ The normal function of the thyroid hormone (TH) system is essential for growth, development and metabolism in humans as well as in other species. The action of TH is dependent on its binding to thyroid hormone receptors (THR) found in the cell nucleus. In some situations, chemicals with structural similarities to TH can bind to these receptors and disrupt their normal function. It has been previously demonstrated that environmental contaminants including, carbamazapine, nonlyphenol (NP), bisphenol A (BPA), and several others are able to bind to the THR as either agonists or antagonists and modulate downstream biochemical responses. Municipal wastewater effluent (MWWE) is a major source of these contaminants entering aquatic environments. Recently extracts of MWWE have been shown to contain chemicals that are capable of binding to THRs. However, MWWE is a complex mixture of chemicals and the specific chemicals have not been identified. In this thesis, a proof of concept was developed for using an Effects Directed Assessment (EDA) approach to isolate thyroid receptor active compounds in MWWE. An EDA is a technique created to extract and identify chemicals from complex mixtures, using various fractionation methods. Once these chemicals have been identified, they are further reviewed for biological relevance. A competitive binding assay for THR was developed and applied to determine the relative binding affinity of known environmental contaminants to THR. Nuclear thyroid hormone receptors were isolated from rainbow trout liver by differential centrifugation. This method involved liver tissue homogenization and subsequent centrifugations to separate the nuclear fraction containing the receptors. The binding characteristics of the isolated THR were evaluated using the thyroid hormones triiodothyronine (T3) and thyroxine (T4) in a competitive binding assay. Minimal binding affinity was present in this assay and future studies should validate the assay further and assure that it is comparable to literature values. Environmental contaminants, including BPA, NP were also tested to determine their relative binding affinity to the THRs compared to the endogenous hormones. High concentrations of both BPA and NP bound to the thyroid hormone receptor, displacing radiolabeled T3 from its binding site. The rainbow trout competitive binding assay was also used to test the binding affinities of extracts from two municipal wastewater effluents collected in the Grand River watershed in southern Ontario. Effluents were extracted using a solid phase adsorbent (HLB Oasis cartridge), eluted with methanol, taken to dryness then reconstituted in ethanol for use in the assay. Both effluent extracts displaced the binding of radiolabeled T3 to the thyroid receptors. The studies demonstrate that a competitive THR assay can be used to detect chemicals in complex mixtures with the potential to interact with THRs. The next step should be to apply the assay using an EDA approach to isolate and identify specific chemicals in effluents that are…
Subjects/Keywords: Thyroid; Receptors
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Sethi, N. (2012). The interaction of chemicals isolated from municipal wastewater effluent with rainbow trout (Oncorhynchus mykiss) thyroid hormone receptors. (Thesis). University of Waterloo. Retrieved from http://hdl.handle.net/10012/7126
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Sethi, Neha. “The interaction of chemicals isolated from municipal wastewater effluent with rainbow trout (Oncorhynchus mykiss) thyroid hormone receptors.” 2012. Thesis, University of Waterloo. Accessed March 02, 2021.
http://hdl.handle.net/10012/7126.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Sethi, Neha. “The interaction of chemicals isolated from municipal wastewater effluent with rainbow trout (Oncorhynchus mykiss) thyroid hormone receptors.” 2012. Web. 02 Mar 2021.
Vancouver:
Sethi N. The interaction of chemicals isolated from municipal wastewater effluent with rainbow trout (Oncorhynchus mykiss) thyroid hormone receptors. [Internet] [Thesis]. University of Waterloo; 2012. [cited 2021 Mar 02].
Available from: http://hdl.handle.net/10012/7126.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Sethi N. The interaction of chemicals isolated from municipal wastewater effluent with rainbow trout (Oncorhynchus mykiss) thyroid hormone receptors. [Thesis]. University of Waterloo; 2012. Available from: http://hdl.handle.net/10012/7126
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Rutgers University
3.
Halikere, Apoorva, 1989-.
Synaptic mechanisms of OPRM1 A118G single nucleotide polymorphism in human neurons.
Degree: PhD, Neuroscience, 2018, Rutgers University
URL: https://rucore.libraries.rutgers.edu/rutgers-lib/57584/
► Association of the non-synonymous single nucleotide polymorphism (SNP) rs1799971 in OPRM1 to drug dependence and alcohol abuse suggests it may have a functional consequence in…
(more)
▼ Association of the non-synonymous single nucleotide polymorphism (SNP) rs1799971 in OPRM1 to drug dependence and alcohol abuse suggests it may have a functional consequence in altering receptor signaling in the brain. The A118G SNP causes a switch of asparagine (N) at position 40 of the mu-opioid receptor (MOR) to aspartate (D). To dissect the underlying neural and synaptic basis of the N40D MOR variant, we generated human GABAergic induced neuronal (iN) cells from induced pluripotent stem (iPS) cells of donors homozygous for either the major (N40) or minor (D40) alleles of the MOR. We found that the subject-derived iN cells exhibit mature neuronal properties such as action potential firing and neuronal excitability and express functional MORs. Interestingly, upon MOR activation by the agonist DAMGO, D40 MOR iN cells exhibit consistently stronger suppression of spontaneous inhibitory postsynaptic currents (sIPSCs) than N40 MOR iN cells across multiple subjects. To mitigate the complexity of diverse genetic backgrounds of the subject iN cells derived from multiple human subjects, we employed CRISPR/Cas9 genome-editing to generate two pairs of isogenic human pluripotent stem cell lines. Remarkably, the synaptic regulation of MOR activation in the isogenic lines recapitulate those of neurons generated from different individuals, i.e. stronger suppression in D40 MOR carrying human neuronal cells by MOR activation. We further determined that the increased sensitivity of D40 iN cells to DAMGO was caused by a more robust inhibition of excitability and synaptic release by DAMGO in D40 MOR expressing neurons. Additionally, we found that the N40D SNP influences the development of long-term tolerance at the MOR. Specifically, D40 iN cells are unable to develop adaptive changes in synaptic function unlike N40 iN cells following long-term mu opioid receptor activation by DAMGO. This study utilizes patient-specific iPS cells as well as a gene edited isogenic neurons to advance our understanding of the fundamental synaptic alterations associated with OPRM1 A118G in a human neuronal context.
Advisors/Committee Members: Pang, Zhiping (chair), Pintar, John E (internal member), Hart, Ronald P (internal member), Blendy, Julie A (outside member), School of Graduate Studies.
Subjects/Keywords: Opioids – Receptors
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Halikere, Apoorva, 1. (2018). Synaptic mechanisms of OPRM1 A118G single nucleotide polymorphism in human neurons. (Doctoral Dissertation). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/57584/
Chicago Manual of Style (16th Edition):
Halikere, Apoorva, 1989-. “Synaptic mechanisms of OPRM1 A118G single nucleotide polymorphism in human neurons.” 2018. Doctoral Dissertation, Rutgers University. Accessed March 02, 2021.
https://rucore.libraries.rutgers.edu/rutgers-lib/57584/.
MLA Handbook (7th Edition):
Halikere, Apoorva, 1989-. “Synaptic mechanisms of OPRM1 A118G single nucleotide polymorphism in human neurons.” 2018. Web. 02 Mar 2021.
Vancouver:
Halikere, Apoorva 1. Synaptic mechanisms of OPRM1 A118G single nucleotide polymorphism in human neurons. [Internet] [Doctoral dissertation]. Rutgers University; 2018. [cited 2021 Mar 02].
Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/57584/.
Council of Science Editors:
Halikere, Apoorva 1. Synaptic mechanisms of OPRM1 A118G single nucleotide polymorphism in human neurons. [Doctoral Dissertation]. Rutgers University; 2018. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/57584/

University of Alberta
4.
McEown, Kristopher Scott.
The Roles of Mineralocorticoid and GABAA Receptors in
Anxiety and Fear Memory.
Degree: PhD, Department of Psychology, 2013, University of Alberta
URL: https://era.library.ualberta.ca/files/12579t871
► The purpose of this dissertation was to examine the roles of brain mineralocorticosteroid (MR) and γ-aminobutyric acid (GABAA) receptors in mediating unconditioned fear and fear…
(more)
▼ The purpose of this dissertation was to examine the
roles of brain mineralocorticosteroid (MR) and γ-aminobutyric acid
(GABAA) receptors in mediating unconditioned fear and fear memory.
The first set of experiments explored the role of hippocampal and
medial prefrontal cortex mineralocorticosteroid receptors (MRs) in
anxiety and fear memory. The MR antagonist RU28318 was microinfused
into the dorsal hippocampus (DH), ventral hippocampus (VH) or
medial prefrontal cortex (mPFC) ten minutes prior to testing in two
rodent models of unconditioned anxiety, the elevated plus-maze and
shock-probe burying test. Fear memory was then assessed in the
shock-probe apparatus 24 hours later by re-exposing non-drugged
rats to a non-electrified probe. RU28318 infusions into the VH
reduced anxiety in the elevated plus-maze while RU28318 in the DH
or mPFC did not. In contrast, RU28318 infusions into the DH, VH and
mPFC all reduced anxiety in the shock-probe burying test. Fear
memory was not affected by infusions into any of the three brain
regions. The second set of experiments examined the role of
hippocampal GABAA receptor sub-units in mediating anxiety and fear
memory. α2 GABAA receptor sub-units are thought to mediate the
anxiolytic effects of benzodiazepines and α5 sub-units are thought
to mediate the amnesic effects of benzodiazepines. The DH and VH
both contain GABAA receptors having these sub-units. Rats were
given intra-hippocampal microinfusions of either TPA023 (an α2
agonist) or TB-21007 (an α5 inverse agonist) and tested in the
plus-maze and shock-probe tests. Twenty-four hours later, rats were
tested for fear memory with the non-electrified shock-probe. The α2
agonist (TPA023) reduced anxiety when it was infused into the VH
but had no effect when infused into the DH. Conversely, the inverse
α5 agonist (TB-21007) impaired fear memory when it was infused into
the DH, but not when it was infused into the VH. Overall, these
results suggest that mineralocorticoid and GABAA receptors in the
ventral hippocampus mediate anxiety. In addition, these results
suggest that ventral hippocampal GABAA α2 sub-units mediate anxiety
and dorsal hippocampal GABAA α5 sub-units mediate fear
memory.
Subjects/Keywords: Anxiety; Fear Memory; Mineralocorticoid Receptors; GABAA Receptors
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
McEown, K. S. (2013). The Roles of Mineralocorticoid and GABAA Receptors in
Anxiety and Fear Memory. (Doctoral Dissertation). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/12579t871
Chicago Manual of Style (16th Edition):
McEown, Kristopher Scott. “The Roles of Mineralocorticoid and GABAA Receptors in
Anxiety and Fear Memory.” 2013. Doctoral Dissertation, University of Alberta. Accessed March 02, 2021.
https://era.library.ualberta.ca/files/12579t871.
MLA Handbook (7th Edition):
McEown, Kristopher Scott. “The Roles of Mineralocorticoid and GABAA Receptors in
Anxiety and Fear Memory.” 2013. Web. 02 Mar 2021.
Vancouver:
McEown KS. The Roles of Mineralocorticoid and GABAA Receptors in
Anxiety and Fear Memory. [Internet] [Doctoral dissertation]. University of Alberta; 2013. [cited 2021 Mar 02].
Available from: https://era.library.ualberta.ca/files/12579t871.
Council of Science Editors:
McEown KS. The Roles of Mineralocorticoid and GABAA Receptors in
Anxiety and Fear Memory. [Doctoral Dissertation]. University of Alberta; 2013. Available from: https://era.library.ualberta.ca/files/12579t871

University of Ottawa
5.
Hristova, Elitza.
Sigma-1 Receptor (σ – 1R) Activation and Modulation of NMDA Receptor Surface Expression
.
Degree: 2014, University of Ottawa
URL: http://hdl.handle.net/10393/31784
► The sigma-1 receptors (σ-1Rs) are endoplasmic reticulum (ER) resident proteins shown to have chaperone-like functions, and are widely distributed throughout the central nervous system (CNS).…
(more)
▼ The sigma-1 receptors (σ-1Rs) are endoplasmic reticulum (ER) resident proteins shown to have chaperone-like functions, and are widely distributed throughout the central nervous system (CNS). They reside at a specialized membrane called mitochondria- associated ER-membrane (MAM) and can modulate numerous voltage- and ligand-gated ion channels. One of these channels is the N-methyl-D-aspartate receptor (NMDAR), and σ-1R ligands are able to enhance the potentiation of NMDARs, but the mechanism involved remains poorly understood. Using various biochemical techniques, we show that 90 min following an i.p. injection of σ-1R agonists ((+)-SKF 10,047 (SKF), (+)- Pentazocine (PTZ), or PRE-084 (PRE), there is an increase in the expression of GluN2- containing NMDARs in the rat hippocampus. These results suggest that σ-1R activation is able to enhance NMDAR function by modulating protein expression levels both in the cytosol and on the cell surface. This suggests that σ-1Rs could be excellent therapeutic targets for many neurological disorders, and for the development of novel antipsychotics.
Subjects/Keywords: Sigma-1 receptors;
NMDA receptors;
Hippocampus
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Hristova, E. (2014). Sigma-1 Receptor (σ – 1R) Activation and Modulation of NMDA Receptor Surface Expression
. (Thesis). University of Ottawa. Retrieved from http://hdl.handle.net/10393/31784
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Hristova, Elitza. “Sigma-1 Receptor (σ – 1R) Activation and Modulation of NMDA Receptor Surface Expression
.” 2014. Thesis, University of Ottawa. Accessed March 02, 2021.
http://hdl.handle.net/10393/31784.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Hristova, Elitza. “Sigma-1 Receptor (σ – 1R) Activation and Modulation of NMDA Receptor Surface Expression
.” 2014. Web. 02 Mar 2021.
Vancouver:
Hristova E. Sigma-1 Receptor (σ – 1R) Activation and Modulation of NMDA Receptor Surface Expression
. [Internet] [Thesis]. University of Ottawa; 2014. [cited 2021 Mar 02].
Available from: http://hdl.handle.net/10393/31784.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Hristova E. Sigma-1 Receptor (σ – 1R) Activation and Modulation of NMDA Receptor Surface Expression
. [Thesis]. University of Ottawa; 2014. Available from: http://hdl.handle.net/10393/31784
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Central Connecticut State University
6.
Ngu, Tuong, 1988-.
Hydrocortisone acting as an inhibitor of GABA receptor in the red crayfish (Procambarus clarkii) muscle receptor organ.
Degree: Department of Biological Sciences, 2016, Central Connecticut State University
URL: http://content.library.ccsu.edu/u?/ccsutheses,2360
"Submitted in Partial Fulfillment of the Requirements for the Degree of Master of Science in Biological Science with Health Specialization."; Thesis advisor: Mark Jackson.; M.S.,Central Connecticut State University,,2016.;
Advisors/Committee Members: Jackson, Mark E.
Subjects/Keywords: Hydrocortisone.; GABA – Receptors.; Procambarus clarkii.; Muscle receptors.
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Ngu, Tuong, 1. (2016). Hydrocortisone acting as an inhibitor of GABA receptor in the red crayfish (Procambarus clarkii) muscle receptor organ. (Thesis). Central Connecticut State University. Retrieved from http://content.library.ccsu.edu/u?/ccsutheses,2360
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Ngu, Tuong, 1988-. “Hydrocortisone acting as an inhibitor of GABA receptor in the red crayfish (Procambarus clarkii) muscle receptor organ.” 2016. Thesis, Central Connecticut State University. Accessed March 02, 2021.
http://content.library.ccsu.edu/u?/ccsutheses,2360.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Ngu, Tuong, 1988-. “Hydrocortisone acting as an inhibitor of GABA receptor in the red crayfish (Procambarus clarkii) muscle receptor organ.” 2016. Web. 02 Mar 2021.
Vancouver:
Ngu, Tuong 1. Hydrocortisone acting as an inhibitor of GABA receptor in the red crayfish (Procambarus clarkii) muscle receptor organ. [Internet] [Thesis]. Central Connecticut State University; 2016. [cited 2021 Mar 02].
Available from: http://content.library.ccsu.edu/u?/ccsutheses,2360.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Ngu, Tuong 1. Hydrocortisone acting as an inhibitor of GABA receptor in the red crayfish (Procambarus clarkii) muscle receptor organ. [Thesis]. Central Connecticut State University; 2016. Available from: http://content.library.ccsu.edu/u?/ccsutheses,2360
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Hong Kong University of Science and Technology
7.
He, Wei.
Single-particle tracking of acetylcholine receptors and ganglioside GM1 on live cell membranes.
Degree: 2015, Hong Kong University of Science and Technology
URL: http://repository.ust.hk/ir/Record/1783.1-83591
;
https://doi.org/10.14711/thesis-b1477557
;
http://repository.ust.hk/ir/bitstream/1783.1-83591/1/th_redirect.html
► Single-particle tracking was used to monitor 2 highly expressed molecules in live Xenopus muscle cells: the transmembrane ion channel acetylcholine receptor (AChR) and the outer-leaflet…
(more)
▼ Single-particle tracking was used to monitor 2 highly expressed molecules in live Xenopus muscle cells: the transmembrane ion channel acetylcholine receptor (AChR) and the outer-leaflet lipid-raft marker ganglioside GM1. AChRs and GM1s were labeled with quantum dots linked to specific toxins, and up to 200 molecules per frame were concurrently tracked at a high sampling frequency (80 Hz) and over a long duration (30 min). In untreated cells, both AChRs and GM1s diffused without marked confinement within a 6-μm range over a long period. However, their instantaneous diffusion coefficient δ was distributed over a broad range and featured an exponential tail. This dynamic heterogeneity was observed in cells under various conditions. Protein networks underneath the membrane, such as those formed by F-actin and scaffold proteins, were examined for their role in regulating the dynamic heterogeneity; for this analysis, cells were exposed to 5 drugs, and the results showed that whereas ATP depletion homogenized the heterogeneity in δ by eliminating cortical F-actin, reducing the membrane cholesterol content did not exert a major effect. By combining the findings of unconfined diffusion, a broad distribution of δ, and the drastic influence of cortical F-actin disruption, we developed a new hypothesis on membrane organization based on the picket-fence model. Moreover, here we report 2 previously unrecognized behaviors of AChRs: First, AChRs moved linearly toward HB-GAM-coated beads. Our results indicated that AChRs’ linear motion toward the beads is guided by motor proteins, rather than by a vehicle that syncs their step speed. Second, transient coalescence (TC) occurred between AChRs and between GM1s. These TC events indicate that certain immobilization sites exist on the plasma membrane that are likely generated by actin polymerization and cholesterol. The sites were frequently detected between bead-induced immobile AChRs and mobile AChRs, and they might facilitate AChR cluster formation.
Subjects/Keywords: Cell membranes
; Nicotinic receptors
; Acetylcholine
; Receptors
; Gangliosides
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
He, W. (2015). Single-particle tracking of acetylcholine receptors and ganglioside GM1 on live cell membranes. (Thesis). Hong Kong University of Science and Technology. Retrieved from http://repository.ust.hk/ir/Record/1783.1-83591 ; https://doi.org/10.14711/thesis-b1477557 ; http://repository.ust.hk/ir/bitstream/1783.1-83591/1/th_redirect.html
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
He, Wei. “Single-particle tracking of acetylcholine receptors and ganglioside GM1 on live cell membranes.” 2015. Thesis, Hong Kong University of Science and Technology. Accessed March 02, 2021.
http://repository.ust.hk/ir/Record/1783.1-83591 ; https://doi.org/10.14711/thesis-b1477557 ; http://repository.ust.hk/ir/bitstream/1783.1-83591/1/th_redirect.html.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
He, Wei. “Single-particle tracking of acetylcholine receptors and ganglioside GM1 on live cell membranes.” 2015. Web. 02 Mar 2021.
Vancouver:
He W. Single-particle tracking of acetylcholine receptors and ganglioside GM1 on live cell membranes. [Internet] [Thesis]. Hong Kong University of Science and Technology; 2015. [cited 2021 Mar 02].
Available from: http://repository.ust.hk/ir/Record/1783.1-83591 ; https://doi.org/10.14711/thesis-b1477557 ; http://repository.ust.hk/ir/bitstream/1783.1-83591/1/th_redirect.html.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
He W. Single-particle tracking of acetylcholine receptors and ganglioside GM1 on live cell membranes. [Thesis]. Hong Kong University of Science and Technology; 2015. Available from: http://repository.ust.hk/ir/Record/1783.1-83591 ; https://doi.org/10.14711/thesis-b1477557 ; http://repository.ust.hk/ir/bitstream/1783.1-83591/1/th_redirect.html
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of North Carolina – Greensboro
8.
Lingerfelt, Mary A.
Construction and validation of GPR55 active and inactive
state in silico models through the use of biological assays,
mutation data, and structure activity relationships.
Degree: 2016, University of North Carolina – Greensboro
URL: http://libres.uncg.edu/ir/listing.aspx?styp=ti&id=21355
► G-protein coupled receptors (GPCRs) function as both gatekeepers and molecular messengers of the cell. They relay signals that span the cell membrane mediating nearly every…
(more)
▼ G-protein coupled
receptors (GPCRs) function as both
gatekeepers and molecular messengers of the cell. They relay
signals that span the cell membrane mediating nearly every
significant physiological process and currently represent the
target of about 30% of all drugs. The signals they transmit can
arise from a remarkable variety of stimuli which includes, but is
not limited to, photons, neurotransmitters and hormones. GPR55, a
rhodopsin-like (Class A) GPCR, has received a great deal of
attention due to its emerging involvement in a multitude of
physiological processes and its putative identity as a third type
of cannabinoid receptor. Characterizations of GPR55 knock-out mice
reveal a role for the receptor in controlling inflammatory pain,
neuropathic pain, and bone resorption.1 Myriad other studies
indicate that GPR55 activation may play a part in oncogenesis and
metathesis. GPR55 can be found in numerous tissue types throughout
the body and is also highly expressed throughout the cerebellum and
surrounding central nervous system lending credence to the idea
that this receptor may play a more crucial physiological role than
originally thought.2 GPR55 has an extensive physiological profile
and has been shown to respond uniquely to a great number of diverse
compounds. Specifically, it has been shown to recognize many
cannabinoid compounds, including CB1 and CB2 endogenous ligands,
phytocannabinoids and synthetic cannabinoids. Similar to the
ligands of the CB1 and CB2
receptors, the endogenous ligand of
GPR55, lysophosphatidylinositol (LPI), is a lipid-derived
molecule.3 LPI activates ERK1/2 and increases [Ca2+] and, to date,
there has been no evidence that LPI interacts with the other
cannabinoid
receptors. Despite innumerable prospective clinical
uses hinted at by the aforementioned research no low nanomolar
potency ligands of GPR55 have been identified. Nor has there been a
radio-ligand developed to characterize the binding site of this
receptor. Lack of such tools is a great impediment to any forward
progress towards developing the GPR55 receptor as a therapeutic
target for drug design. The following research details the creation
of both a GPR55 active- and a GPR55 inactive- state homology model.
Towards this goal, Chapter I details the background of the
discovery, pharmacological relevance and ligand scope of GPR55. Its
purpose is to establish a framework for the research that follows
and highlight the medical importance of this elusive receptor.
Chapter II describes the synthetic preparation of antagonists of
GPR55 for use in preliminary SAR studies. The original high
throughput screen that lead to the identification of novel GPR55
scaffold chemotypes from the screening of over 300,000 compounds
gave rise to the piperidinyloxadiazolone compound CID23612552 and
the synthetic diversification of what was then dubbed Scaffold 1. A
detailed description of the methods used in the construction of the
updated R and R* state of GPR55 models is handled in Chapter III. A
combination of Conformational Memories4,5 (using the…
Advisors/Committee Members: Patricia Reggio (advisor).
Subjects/Keywords: G proteins – Receptors; Cannabinoids – Receptors; Ligands (Biochemistry)
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Chicago ·
MLA ·
Vancouver ·
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APA (6th Edition):
Lingerfelt, M. A. (2016). Construction and validation of GPR55 active and inactive
state in silico models through the use of biological assays,
mutation data, and structure activity relationships. (Doctoral Dissertation). University of North Carolina – Greensboro. Retrieved from http://libres.uncg.edu/ir/listing.aspx?styp=ti&id=21355
Chicago Manual of Style (16th Edition):
Lingerfelt, Mary A. “Construction and validation of GPR55 active and inactive
state in silico models through the use of biological assays,
mutation data, and structure activity relationships.” 2016. Doctoral Dissertation, University of North Carolina – Greensboro. Accessed March 02, 2021.
http://libres.uncg.edu/ir/listing.aspx?styp=ti&id=21355.
MLA Handbook (7th Edition):
Lingerfelt, Mary A. “Construction and validation of GPR55 active and inactive
state in silico models through the use of biological assays,
mutation data, and structure activity relationships.” 2016. Web. 02 Mar 2021.
Vancouver:
Lingerfelt MA. Construction and validation of GPR55 active and inactive
state in silico models through the use of biological assays,
mutation data, and structure activity relationships. [Internet] [Doctoral dissertation]. University of North Carolina – Greensboro; 2016. [cited 2021 Mar 02].
Available from: http://libres.uncg.edu/ir/listing.aspx?styp=ti&id=21355.
Council of Science Editors:
Lingerfelt MA. Construction and validation of GPR55 active and inactive
state in silico models through the use of biological assays,
mutation data, and structure activity relationships. [Doctoral Dissertation]. University of North Carolina – Greensboro; 2016. Available from: http://libres.uncg.edu/ir/listing.aspx?styp=ti&id=21355
9.
NC DOCKS at The University of North Carolina at Greensboro; Singh, Jagjeet.
A model system for understanding cellular signaling of the cannabinoid CB2 receptor via the inhibitory Gi protein.
Degree: 2014, NC Docks
URL: http://libres.uncg.edu/ir/uncg/f/Singh_uncg_0154D_11501.pdf
► One key signaling pathway in the cellular signaling involving G protein coupled receptors (GPCR) is via heterotrimeric G proteins. The first step in GPCR/G protein…
(more)
▼ One key signaling pathway in the cellular signaling involving G protein coupled receptors (GPCR) is via heterotrimeric G proteins. The first step in GPCR/G protein signaling is the activation of a GPCR by the ligand binding and the next step is the activation of the G protein. Understanding the molecular mechanism behind the GPCR/G protein interactions will help in characterizing this important signaling pathway and should ultimately lead to the design of functionally selective ligands for this larger class of receptors. Earlier, the Reggio group used molecular dynamics simulations to study the activation of the cannabinoid CB2 receptor, a class A GPCR, by its endogenous ligand, 2-arachidonoylglycerol (2-AG) via the lipid bilayer. The goal of the current project was to study the next step in the G-protein mediated signal transduction, when an agonist activated CB2 receptor forms a complex with Gi protein and catalyzes the activation of Gi protein, releasing the guanosine diphosphate (GDP) bound between the ras-like (also known as GTPase domain) and helical domains of the Gá protein. To this end, we report here the CB2 / Gái1â1ã2 complex formation using our 2-AG activated CB2 receptor model. For G protein activation (dissociation of GDP), we hypothesized that GDP release from the ras-like and helical domains of Gái would be triggered by the hydration of GDP. We probed the role of the CB2 receptor interactions with the Gái protein and the resultant progression of GDP hydration. We have seen the number of waters surrounding GDP increase from 16 (t= 0 ns) to 28 waters (t=5 ìs). Two important interactions between the receptor and G-protein appear to lead to the increased hydration of GDP. (1) A hydrophobic interaction occurred between CB2 IC2 loop residue P139 and the Gái hydrophobic pocket residues: V34 (N terminus; L194 (â1 sheet); F196 (â2 sheet); and, F336, T340, I343, I344 (á5 helix) multiple times in our 5 ìs long trajectory. Each time this interaction occurred, an increase in GDP hydration was observed in our simulation. 2) We also observed an IC3 loop interaction with the Gái á4 helix between 1.4 to 1.6 ìs, in which the IC3 loop residue R229 reached to interact with E297 and E298. Taken together, our results show that the intracellular loops play a critical role in the hydration of GDP that should lead to G protein activation.
Subjects/Keywords: G proteins $x Receptors; Cannabinoids $x Receptors
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
NC DOCKS at The University of North Carolina at Greensboro; Singh, J. (2014). A model system for understanding cellular signaling of the cannabinoid CB2 receptor via the inhibitory Gi protein. (Thesis). NC Docks. Retrieved from http://libres.uncg.edu/ir/uncg/f/Singh_uncg_0154D_11501.pdf
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
NC DOCKS at The University of North Carolina at Greensboro; Singh, Jagjeet. “A model system for understanding cellular signaling of the cannabinoid CB2 receptor via the inhibitory Gi protein.” 2014. Thesis, NC Docks. Accessed March 02, 2021.
http://libres.uncg.edu/ir/uncg/f/Singh_uncg_0154D_11501.pdf.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
NC DOCKS at The University of North Carolina at Greensboro; Singh, Jagjeet. “A model system for understanding cellular signaling of the cannabinoid CB2 receptor via the inhibitory Gi protein.” 2014. Web. 02 Mar 2021.
Vancouver:
NC DOCKS at The University of North Carolina at Greensboro; Singh J. A model system for understanding cellular signaling of the cannabinoid CB2 receptor via the inhibitory Gi protein. [Internet] [Thesis]. NC Docks; 2014. [cited 2021 Mar 02].
Available from: http://libres.uncg.edu/ir/uncg/f/Singh_uncg_0154D_11501.pdf.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
NC DOCKS at The University of North Carolina at Greensboro; Singh J. A model system for understanding cellular signaling of the cannabinoid CB2 receptor via the inhibitory Gi protein. [Thesis]. NC Docks; 2014. Available from: http://libres.uncg.edu/ir/uncg/f/Singh_uncg_0154D_11501.pdf
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Oregon State University
10.
Venkataraman, Anand.
In vitro and in vivo approaches for the functional characterization of the scaffold protein, GRASP.
Degree: PhD, Molecular and Cellular Biology, 2009, Oregon State University
URL: http://hdl.handle.net/1957/13654
► Studies using the pluripotent embryonic carcinoma cell line, P19, as a retinoic acid (RA)-responsive model system have been instrumental towards our understanding of the RA-dependent…
(more)
▼ Studies using the pluripotent embryonic carcinoma cell line, P19, as a retinoic acid (RA)-responsive model system have been instrumental towards our understanding of the RA-dependent signaling pathways in development and homeostasis. Grp1-associated scaffold protein (GRASP; also known as Tamalin) was first identified by our group, as a gene robustly induced by RA treatment in P19 cells. GRASP was reported to influence the cell-surface expression of interacting membrane
receptors in vitro albeit by an unknown mechanism. Furthermore, the in vivo role of GRASP is poorly understood. Mice with a germline deletion of Grasp (GRASP-/-) do not exhibit any gross morphological, behavioral or sexual deficits and exhibit mild, altered sensitivities to morphine and cocaine administration. The goals of our studies herein were to investigate the mechanistic details of GRASP for the role in trafficking and cell-surface, localization of membrane
receptors in vitro and the role of GRASP in vivo.
Previously, our group had identified a strong interaction of GRASP with Grp1, a guanine nucleotide exchange factor for the small G protein, Arf6. In this study we show GRASP localizes in intracellular recycling endosomal compartment(s), recruits Grp1 to these structures, and facilitates activation of Arf6. Activation of Arf6 regulates key aspects of vesicular trafficking, actin reorganization and cellular migration. Furthermore, we show that GRASP preferentially regulates intracellular membrane trafficking by the Arf6-dependent/clathrin-independent pathway.
We have shown that GRASP is expressed in the adult murine skin. Our studies demonstrate a robust induction of GRASP transcripts in murine dermis and epidermis following exposure to ultraviolet (UV) rays. We also report the generation of novel mice with germline deletion of Grasp (GRASP-/-), that exhibit altered proliferative and apoptotic responses to UV exposure. Ongoing investigations suggest that the observed phenotypes of GRASP-/- mice after UV exposure maybe due to the dysregulation of the subcellular localization of the tumor suppressor protein, p53.
Taken together, our in vitro and in vivo approaches have provided new insight in the role of GRASP as scaffold protein that regulates intracellular trafficking pathways.
Advisors/Committee Members: Leid, Mark (advisor), Kioussi, Chrissa (committee member).
Subjects/Keywords: Trafficking; Cell receptors
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Venkataraman, A. (2009). In vitro and in vivo approaches for the functional characterization of the scaffold protein, GRASP. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/13654
Chicago Manual of Style (16th Edition):
Venkataraman, Anand. “In vitro and in vivo approaches for the functional characterization of the scaffold protein, GRASP.” 2009. Doctoral Dissertation, Oregon State University. Accessed March 02, 2021.
http://hdl.handle.net/1957/13654.
MLA Handbook (7th Edition):
Venkataraman, Anand. “In vitro and in vivo approaches for the functional characterization of the scaffold protein, GRASP.” 2009. Web. 02 Mar 2021.
Vancouver:
Venkataraman A. In vitro and in vivo approaches for the functional characterization of the scaffold protein, GRASP. [Internet] [Doctoral dissertation]. Oregon State University; 2009. [cited 2021 Mar 02].
Available from: http://hdl.handle.net/1957/13654.
Council of Science Editors:
Venkataraman A. In vitro and in vivo approaches for the functional characterization of the scaffold protein, GRASP. [Doctoral Dissertation]. Oregon State University; 2009. Available from: http://hdl.handle.net/1957/13654

University of Utah
11.
Roalstad, Shelly M.
Receptor dynamics in virtual screening: A dihydrofolate reductase case study.
Degree: MS;, Pharmaceutics & Pharmaceutical Chemistry;, 2007, University of Utah
URL: http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/1634/rec/956
► The implications of receptor dynamics virtual screening were explored as part of proof of concept study for an interactive docking, molecular dynamics and scoring strategy.…
(more)
▼ The implications of receptor dynamics virtual screening were explored as part of proof of concept study for an interactive docking, molecular dynamics and scoring strategy. The original study was to demonstrate whether or not the iterative process proposed would relax receptor structures that began as apo (without drug) or homology models toward known holo (with drug) coordinates. Due to species differences in the original receptor test systems, the study was refined to a set of well characterized dihydrofolate reductase (DHFR) receptors. Since both apo and holo structures were not available for each DHFR receptor selected, the goal of relaxing an apo structure toward a holo structure was modified. Various complexes of enzymes, ligand and cofactor were constructed to represent intermediates in the catalytic cycle know to adopt distinct conformations, and the new goal was to demonstrate movement toward one of these conformations during molecular dynamics simulations. Additionally, drug resistant E. coli DHFR and human DHFR structures were selected to probe the possible flexibility differences giving rise to enzymatic activity. Traditional molecular dynamics simulations demonstrated correct conformational change for one enzyme cofactor complex after 70 nanoseconds of simulation. Overall, relatively few complexes experienced any conformational change, indicating the need for longer trajectories or more advanced simulation techniques. Flexible regions of the enzyme described in literature were correctly depicted by trajectory analysis. Flexibility differences between bacterial and vertebrate DHFR showed bacterial DHFR to have more localized movements of three flexible loops and vertebrate DHFR more disperse flexibility. Evaluating the free energy of binding by Molecular Mechanics – Poisson Boltzmann Surface Area (MM-PBSA) methods was shown to be more insightful when employed before and after conformational change rather than over an entire trajectory. Ligand binding values generally independent of enzyme activity demonstrated the necessity of considering binding in the context of the catalytic cycle. Traditional molecular dynamics demonstrated the capability of moving a receptor toward a known conformation, through the resolution of conformational change was depended on the amount of trajectory available. Advanced molecular dynamics methods possible capable of expediting conformational change are proposed for future studies.
Subjects/Keywords: Drug Receptors; Drugs:
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Roalstad, S. M. (2007). Receptor dynamics in virtual screening: A dihydrofolate reductase case study. (Masters Thesis). University of Utah. Retrieved from http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/1634/rec/956
Chicago Manual of Style (16th Edition):
Roalstad, Shelly M. “Receptor dynamics in virtual screening: A dihydrofolate reductase case study.” 2007. Masters Thesis, University of Utah. Accessed March 02, 2021.
http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/1634/rec/956.
MLA Handbook (7th Edition):
Roalstad, Shelly M. “Receptor dynamics in virtual screening: A dihydrofolate reductase case study.” 2007. Web. 02 Mar 2021.
Vancouver:
Roalstad SM. Receptor dynamics in virtual screening: A dihydrofolate reductase case study. [Internet] [Masters thesis]. University of Utah; 2007. [cited 2021 Mar 02].
Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/1634/rec/956.
Council of Science Editors:
Roalstad SM. Receptor dynamics in virtual screening: A dihydrofolate reductase case study. [Masters Thesis]. University of Utah; 2007. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/1634/rec/956

University of Utah
12.
Osborne, Amber Valeria.
The Modulatory Role of Neuronal Nicotinic Receptors on Peripheral Inflammation.
Degree: PhD, Pathology;, 2010, University of Utah
URL: http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/1089/rec/1195
► This dissertation examines the relationship between inflammatory cytokines and nicotinic acetylcholine receptors (nAChR). WT and nAChRa7 null mice were exposed to 4.8kJ/m2 of UVB on…
(more)
▼ This dissertation examines the relationship between inflammatory cytokines and nicotinic acetylcholine receptors (nAChR). WT and nAChRa7 null mice were exposed to 4.8kJ/m2 of UVB on a standardized area of back skin. This dose of UVB caused the skin to be visibly inflamed 48 hours after exposure; at this point the back skin was removed and analyzed for inflammatory cytokines. Production of IL-lp and IL-6, but not TNFa was significantly increased in a 7 null mice. Further, H&E stained skin sections revealed greater edema in mice lacking a7. Production of SOCS3, a negative regulator of cytokines, was decreased in inflamed skin from a7 null mice, suggesting a potential mechanism by which a7 could regulate cytokine production. The induction of IL-1(3 and IL-6 in skin by UVB was inhibited in mice first exposed to y-irradiation, suggesting that rapidly dividing cells that infiltrate the inflamed skin were responsible for the measured cytokine production. To further identify the types of cells infiltrating the skin following an inflammatory stimulus, croton oil was applied to mouse ears and infiltrating cells were isolated following the separation of dorsal and ventral ear flaps. Mice lacking a7 had a significant increase in the total number of recruited cells as well as increased expression of IL-lp, IL-6 and TNFa by infiltrating cells. The cells infiltrating the inflamed site were determined to be CD1 lb+ Ly6g+ neutrophils and CD1 lb+ F4/80+ macrophages by FACS analysis. Further, it was determined that chemokines which recruit neutrophils and macrophages, such as CXCL2, CXCL5 and CCL3 were all also increased in a7 null mice. Inhibiting the p38 MAP Kinase signaling pathway in WT and a 7 null primary macrophage cultures decreased the LPS induced expression of cytokines and chemokines. The reciprocal effect of cytokines on nicotinic receptors was also investigated. It was determined that treatment of transfected HEK293 cells with TNFa induces enhanced upregulation of the high affinity nicotine binding receptor a4p2. Treatment of these cells with a p38 MAP Kinase inhibitor blocks the TNFa enhanced upregulation. Collectively, these data demonstrate a relationship between nicotinic receptors and inflammatory cytokines and the involvement of the p38 MAP Kinase pathway.
Subjects/Keywords: Nicotine Receptors; Inflammation
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Osborne, A. V. (2010). The Modulatory Role of Neuronal Nicotinic Receptors on Peripheral Inflammation. (Doctoral Dissertation). University of Utah. Retrieved from http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/1089/rec/1195
Chicago Manual of Style (16th Edition):
Osborne, Amber Valeria. “The Modulatory Role of Neuronal Nicotinic Receptors on Peripheral Inflammation.” 2010. Doctoral Dissertation, University of Utah. Accessed March 02, 2021.
http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/1089/rec/1195.
MLA Handbook (7th Edition):
Osborne, Amber Valeria. “The Modulatory Role of Neuronal Nicotinic Receptors on Peripheral Inflammation.” 2010. Web. 02 Mar 2021.
Vancouver:
Osborne AV. The Modulatory Role of Neuronal Nicotinic Receptors on Peripheral Inflammation. [Internet] [Doctoral dissertation]. University of Utah; 2010. [cited 2021 Mar 02].
Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/1089/rec/1195.
Council of Science Editors:
Osborne AV. The Modulatory Role of Neuronal Nicotinic Receptors on Peripheral Inflammation. [Doctoral Dissertation]. University of Utah; 2010. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/1089/rec/1195
13.
Visic, Petra.
The role of the Sigma1 receptor (σ1R) in store-operated calcium entry (SOCE) microdomains.
Degree: PhD, 2020, University of Sussex
URL: http://sro.sussex.ac.uk/id/eprint/90959/
;
https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.804978
► The Sigma1 receptor (σ1R) is an endoplasmic reticulum (ER) chaperone targeted to mitochondrion associated ER membranes (MAMs). The upregulation of σ1R is associated with various…
(more)
▼ The Sigma1 receptor (σ1R) is an endoplasmic reticulum (ER) chaperone targeted to mitochondrion associated ER membranes (MAMs). The upregulation of σ1R is associated with various cancers, whereas the decreased expression and mutant forms of σ1R are associated with neurodegenerative disorders. The underlying cellular mechanism by which σ1R exerts such a versatile impact remains elusive. At MAMs, σ1R is reported to regulate inter-organelle Ca2+ levels. Following translocation to ER-plasma membrane junctions, σ1R is reported to interact with various ion channels, including the key regulators of store-operated Ca2+ entry (SOCE). I have investigated the role of σ1R in the regulation of SOCE within a population of cells and within the SOCE microdomains of single cells. In a population of cells, overexpression of σ1R profoundly inhibited both Ca2+ store content and SOCE. Although these measurements of global [Ca2+]cyt provide essential information about the functional role of σ1R in the regulation of SOCE, they do not provide detailed information about the effects of σ1R on temporal and spatial aspects of SOCE within the microdomain of the ER-plasma membrane junction. Recently developed, the G-GECO1.2-Orai1 construct encodes for a fully functional Orai1 protein with a genetically encoded fluorescent Ca2+ reporter protein. The Orai1 channel is a key regulator of SOCE. GECO1.2-Orai1 allows for the monitoring of Orai1 channel activity in SOCE microdomains. Within the SOCE microdomains, overexpression of σ1R reduced the frequency of highly-activated G-GECO1.2-Orai1 clusters, which resulted in cumulatively low SOCE. Overexpression of σ1RE102Q, an ALS-causing mutant, failed to inhibit SOCE. In situ, σ1R was shown to interact with another key regulator of SOCE, STIM1 and its less-studied homolog STIM2. Overexpression of σ1R resulted in the inhibition of STIM2.2-mediated SOCE triggered by the ER Ca2+ store depletion. σ1R failed to inhibit STIM2.2-mediated SOCE during basal conditions, suggesting that regulation of SOCE by σ1R requires a greater drop in ER [Ca2+] than occurs under basal conditions.
Subjects/Keywords: RM0301.41 Drug receptors
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Visic, P. (2020). The role of the Sigma1 receptor (σ1R) in store-operated calcium entry (SOCE) microdomains. (Doctoral Dissertation). University of Sussex. Retrieved from http://sro.sussex.ac.uk/id/eprint/90959/ ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.804978
Chicago Manual of Style (16th Edition):
Visic, Petra. “The role of the Sigma1 receptor (σ1R) in store-operated calcium entry (SOCE) microdomains.” 2020. Doctoral Dissertation, University of Sussex. Accessed March 02, 2021.
http://sro.sussex.ac.uk/id/eprint/90959/ ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.804978.
MLA Handbook (7th Edition):
Visic, Petra. “The role of the Sigma1 receptor (σ1R) in store-operated calcium entry (SOCE) microdomains.” 2020. Web. 02 Mar 2021.
Vancouver:
Visic P. The role of the Sigma1 receptor (σ1R) in store-operated calcium entry (SOCE) microdomains. [Internet] [Doctoral dissertation]. University of Sussex; 2020. [cited 2021 Mar 02].
Available from: http://sro.sussex.ac.uk/id/eprint/90959/ ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.804978.
Council of Science Editors:
Visic P. The role of the Sigma1 receptor (σ1R) in store-operated calcium entry (SOCE) microdomains. [Doctoral Dissertation]. University of Sussex; 2020. Available from: http://sro.sussex.ac.uk/id/eprint/90959/ ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.804978

Nelson Mandela Metropolitan University
14.
Van Zyl, Dwain George.
Production of recombinant human CD21 and CD23 : towards a better understanding of their interaction.
Degree: MSc, Faculty of Science, 2013, Nelson Mandela Metropolitan University
URL: http://hdl.handle.net/10948/d10211135
► The prevalence of allergic diseases has dramatically increased over the last three decades. Presently, it is estimated that 20-30 per cent of the developed world…
(more)
▼ The prevalence of allergic diseases has dramatically increased over the last three decades. Presently, it is estimated that 20-30 per cent of the developed world suffers from allergic diseases. The majority of allergic diseases are rooted in the activities of IgE; an immunoglobulin which exerts its effector functions by interacting with a network of proteins. This network includes its low affinity receptor CD23. Cross linking of membrane IgE and CD21 by soluble CD23 results in an increase in IgE synthesis. This marks the interaction between CD23 and CD21 as an attractive therapeutic target. However, details regarding this interaction are inadequate for rational drug design. To obtain a deeper understanding of the CD23-CD21 interaction recombinant human CD21 (SCR1-2 and SCR5-8) and CD23 (16 kD and 25 kDa) were produced. The cloning, expression and purification of recombinant proteins comprised a significant portion of this study. Recombinant CD23 was expressed as inclusion bodies, refolded by rapid dilution and purified by size exclusion chromatography. Conversely, recombinant CD21 was expressed as soluble MBP-fusions and purified with an amylose affinity resin. The interaction between recombinant CD23 and CD21 was analysed by flow cytometry and ELISA experiments. Flow cytometry showed that 16 kDa and 25 kDa CD23 interacted with SCR5-8 to the same extent. Semi-quantitative ELISA experiments showed that both SCR1-2 and SCR5-8 were able to interact with 16 kDa and 25 kDa CD23. This suggests that the binding sites of SCR1-2 and SCR5-8 occur on 16 kDa CD23. Furthermore, since proteins were expressed in E. coli it suggests that the CD23-CD21 interaction does not require glycosylation. Furthermore, considering what is known about the SCR1-2-CD23 interaction from previous NMR studies; i.e. that the C-terminal tail (residues residues 289-298) of CD23 is responsible for binding SCR1-2, indicates that SCR5-8 binds somewhere within the lectin domain of CD23. This indicates that the CD23-CD21 interaction involves C-terminal tail-SCR1-2 and lectin domain-SCR5-8 interactions.
Advisors/Committee Members: Oosthuizen, V Dr.
Subjects/Keywords: Immunoglobulins; Fc receptors
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Van Zyl, D. G. (2013). Production of recombinant human CD21 and CD23 : towards a better understanding of their interaction. (Masters Thesis). Nelson Mandela Metropolitan University. Retrieved from http://hdl.handle.net/10948/d10211135
Chicago Manual of Style (16th Edition):
Van Zyl, Dwain George. “Production of recombinant human CD21 and CD23 : towards a better understanding of their interaction.” 2013. Masters Thesis, Nelson Mandela Metropolitan University. Accessed March 02, 2021.
http://hdl.handle.net/10948/d10211135.
MLA Handbook (7th Edition):
Van Zyl, Dwain George. “Production of recombinant human CD21 and CD23 : towards a better understanding of their interaction.” 2013. Web. 02 Mar 2021.
Vancouver:
Van Zyl DG. Production of recombinant human CD21 and CD23 : towards a better understanding of their interaction. [Internet] [Masters thesis]. Nelson Mandela Metropolitan University; 2013. [cited 2021 Mar 02].
Available from: http://hdl.handle.net/10948/d10211135.
Council of Science Editors:
Van Zyl DG. Production of recombinant human CD21 and CD23 : towards a better understanding of their interaction. [Masters Thesis]. Nelson Mandela Metropolitan University; 2013. Available from: http://hdl.handle.net/10948/d10211135

Texas Tech University
15.
Lokubandara, Anuththara Hemamali.
Computational and functional analysis of human bitter taste receptors.
Degree: MS, Biotechnology, 2018, Texas Tech University
URL: http://hdl.handle.net/2346/73804
► Mammals perceive five tastes: sweet, umami, salty, sour, and bitter. The focus of this study is related to the sense of taste or gustation. The…
(more)
▼ Mammals perceive five tastes: sweet, umami, salty, sour, and bitter. The focus of this study is related to the sense of taste or gustation. The sense of taste enables an animal to discriminate nourishment from toxins. The sense of taste is mediated by taste receptor cells. These cells are localized and organized within taste buds. Taste receptor cells are primarily found within the oral cavity, typically on the dorsal surface of the tongue, palate, the oropharynx. There are two taste receptor protein families in mammals: T1R and T2R. Taste
receptors are members of the G-protein coupled receptor super family (GPCR). Sequence homology assessments of these
receptors place them in two categories: the T1R family is classified as class C; whereas, T2Rs are categorized as class A GPCRs. Twenty-Five T2Rs or bitter taste
receptors have been identified in humans. Bitter taste
receptors are characterized by seven transmembrane domains along with short amino and carboxyl termini-Class A GPCRs.
Most of the research carried out thus far has focused on sweet and umami taste
receptors. Bitter taste receptor studies have focused on
receptors with known ligands that can activate them. The objective of this study is to elucidate the mechanism of the interactions between bitter taste
receptors and tastants (or compounds that elicit a response from a bitter taste receptor). We utilized high-throughput and computationally-derived processes have been utilized to build models for a rational set of
receptors; subsequently, we docked the experimentally known agonists with each receptor.
Two strategies were adopted for docking-targeted and automatic. Theoretically, approximately, nine hundred receptor-ligand interactions in this high throughput endeavor were assessed. Realistically however, five hundred and seventy-nine interactions were feasible and available for further analysis. Automated docking was not only observed to be always energetically favorable, but also a high number of conformations were observed when compared to targeted docking. Specific residues were identified associated with many ligands for a specific receptor. Common residues were observed to be associated with similar functional groups of ligands.
Advisors/Committee Members: Tripathy, Jatindra N (committee member), San-Francisco, Susan (committee member), Crasto, Chiquito J. (Committee Chair).
Subjects/Keywords: Bitter Taste Receptors
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MLA ·
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APA (6th Edition):
Lokubandara, A. H. (2018). Computational and functional analysis of human bitter taste receptors. (Masters Thesis). Texas Tech University. Retrieved from http://hdl.handle.net/2346/73804
Chicago Manual of Style (16th Edition):
Lokubandara, Anuththara Hemamali. “Computational and functional analysis of human bitter taste receptors.” 2018. Masters Thesis, Texas Tech University. Accessed March 02, 2021.
http://hdl.handle.net/2346/73804.
MLA Handbook (7th Edition):
Lokubandara, Anuththara Hemamali. “Computational and functional analysis of human bitter taste receptors.” 2018. Web. 02 Mar 2021.
Vancouver:
Lokubandara AH. Computational and functional analysis of human bitter taste receptors. [Internet] [Masters thesis]. Texas Tech University; 2018. [cited 2021 Mar 02].
Available from: http://hdl.handle.net/2346/73804.
Council of Science Editors:
Lokubandara AH. Computational and functional analysis of human bitter taste receptors. [Masters Thesis]. Texas Tech University; 2018. Available from: http://hdl.handle.net/2346/73804
16.
Chew, Geoffrey H.
Manipulation of α-Bungarotoxin Binding Sites in Nicotinic
Acetylcholine Receptors to Elucidate Fundamental Aspects of
Receptor Function.
Degree: PhD, Molecular Pharmacology, Physiology, and
Biotechnology, 2011, Brown University
URL: https://repository.library.brown.edu/studio/item/bdr:11163/
► Nicotinic acetylcholine receptors (nAChRs) mediate excitatory neurotransmission at synapses throughout the body in the neuromuscular junction and the central nervous system. The two basic types…
(more)
▼ Nicotinic acetylcholine
receptors (nAChRs) mediate
excitatory neurotransmission at synapses throughout the body in the
neuromuscular junction and the central nervous system. The two
basic types of nAChRs that have been identified and studied are the
muscle nAChR and the neuronal nAChR. The neurotoxin α-bungarotoxin
(Bgtx), found in the snake Bungarus multicinctus, specifically
blocks muscle nAChRs and has greatly facilitated our understanding
of these
receptors in muscle cells. Bgtx has been used to study
subunit configuration and conformation, agonist and antagonist
binding, and receptor trafficking in the cell. However, many
subtypes of the neuronal nAChR are not blocked by Bgtx and thus
cannot be directly detected with Bgtx binding studies. One way to
solve this problem is to introduce a Bgtx binding site into
subunits that are not normally sensitive to Bgtx. Adding this site
will allow for localization of nAChR subunits in the brain and
improve our ability to study their functional properties. In this
proposal, I put forth experiments to study the effect of mutations
of neuronal nAChR subunits that introduce Bgtx binding sites and
discuss how this approach will aid our understanding of
nAChRs.
Advisors/Committee Members: Hawrot, Edward (Director), Bowen, Wayne (Reader), Zimmerman, Anita (Reader), Burwell, Rebecca (Reader), Leite-Morris, Kimberly (Reader).
Subjects/Keywords: nicotinic acetylcholine receptors
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Chew, G. H. (2011). Manipulation of α-Bungarotoxin Binding Sites in Nicotinic
Acetylcholine Receptors to Elucidate Fundamental Aspects of
Receptor Function. (Doctoral Dissertation). Brown University. Retrieved from https://repository.library.brown.edu/studio/item/bdr:11163/
Chicago Manual of Style (16th Edition):
Chew, Geoffrey H. “Manipulation of α-Bungarotoxin Binding Sites in Nicotinic
Acetylcholine Receptors to Elucidate Fundamental Aspects of
Receptor Function.” 2011. Doctoral Dissertation, Brown University. Accessed March 02, 2021.
https://repository.library.brown.edu/studio/item/bdr:11163/.
MLA Handbook (7th Edition):
Chew, Geoffrey H. “Manipulation of α-Bungarotoxin Binding Sites in Nicotinic
Acetylcholine Receptors to Elucidate Fundamental Aspects of
Receptor Function.” 2011. Web. 02 Mar 2021.
Vancouver:
Chew GH. Manipulation of α-Bungarotoxin Binding Sites in Nicotinic
Acetylcholine Receptors to Elucidate Fundamental Aspects of
Receptor Function. [Internet] [Doctoral dissertation]. Brown University; 2011. [cited 2021 Mar 02].
Available from: https://repository.library.brown.edu/studio/item/bdr:11163/.
Council of Science Editors:
Chew GH. Manipulation of α-Bungarotoxin Binding Sites in Nicotinic
Acetylcholine Receptors to Elucidate Fundamental Aspects of
Receptor Function. [Doctoral Dissertation]. Brown University; 2011. Available from: https://repository.library.brown.edu/studio/item/bdr:11163/

Hong Kong University of Science and Technology
17.
Ren, Lihuan.
GABAA receptor subtype selectivity and structure-activity relationships of flavonoids.
Degree: 2011, Hong Kong University of Science and Technology
URL: http://repository.ust.hk/ir/Record/1783.1-7346
;
https://doi.org/10.14711/thesis-b1155315
;
http://repository.ust.hk/ir/bitstream/1783.1-7346/1/th_redirect.html
► A flavonoid, 6-hydroxyflavone, was previously reported to bind to the benzodiazepine (BZ) site on GABAA receptors with moderate binding affinity. In the present study, we…
(more)
▼ A flavonoid, 6-hydroxyflavone, was previously reported to bind to the benzodiazepine (BZ) site on GABAA receptors with moderate binding affinity. In the present study, we showed that 6-hydroxyflavone partially potentiated GABA-induced currents in native GABAA receptors expressed in cortical neurons via BZ site, as the enhancement was blocked by the antagonist flumazenil. Furthermore, in patch clamp studies, 6-hydroxyflavone displayed significant preference for α2- and α3-containing subtypes, which were thought to mediate anxiolytic effect, compared to α1- and α5-containing subtypes expressed in HEK 293T cells. In mice, 6-hydroxyflavone exhibited anxiolytic-like effect in the elevated plus-maze test, unaccompanied by the sedative, cognitive impairing, myorelaxant, motor incoordination and anticonvulsant effects when tested in the hole-board, step-through passive avoidance, horizontal wire, rotarod, and pentylenetetrazol (PTZ)-induced seizure tests, respectively. The findings therefore identified 6-hydroxyflavone as a promising drug candidate for the treatment of anxiety-like disorders. In addition, GABAA receptor structure-efficacy relationships for flavones were also studied. The present study focused on flavone 6-substitution, implied in previous studies being relevant to efficacy. Structure analogues, each varying only at position 6, were compared, including 6-fluoroflavone, 6-chloroflavone, 6-bromoflavone, and 2’-hydroxyflavone analyzed in the present study, as well as 6,2’-dihydroxyflavone reported earlier. Whole-cell patch clamp and animal behavior experiments demonstrated 6-bromoflavone to be a positive modulator at GABAA receptors acting through benzodiazepine site. In contrast, the other two 6-haloflavones were both neutralizing modulators. Moreover, 2’-hydroxyflavone was shown to be a neutralizing modulator, different in efficacy from its structural analogue, 6,2’-dihydroxyflavone, a negative modulator of GABAA receptors. The fact that flavone analogues differing only at position 6 showed drastically different pharmacological properties clearly points to 6-substitution being an important determinant of efficacy. The results suggest that a large width of the first atom on the 6-substituent favors a high binding affinity of the 6-substituted flavone, whereas a large overall volume of the 6-substituent favors positive modulator activity, which could be modified by, e.g., 2’-hydroxyl substitution. These findings have contributed to the understanding of quantitative structure-efficacy relationships for flavones acting at GABAA receptors, and hence facilitation of flavone-based drug development.
Subjects/Keywords: GABA – Receptors
; Flavonoids
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Ren, L. (2011). GABAA receptor subtype selectivity and structure-activity relationships of flavonoids. (Thesis). Hong Kong University of Science and Technology. Retrieved from http://repository.ust.hk/ir/Record/1783.1-7346 ; https://doi.org/10.14711/thesis-b1155315 ; http://repository.ust.hk/ir/bitstream/1783.1-7346/1/th_redirect.html
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Ren, Lihuan. “GABAA receptor subtype selectivity and structure-activity relationships of flavonoids.” 2011. Thesis, Hong Kong University of Science and Technology. Accessed March 02, 2021.
http://repository.ust.hk/ir/Record/1783.1-7346 ; https://doi.org/10.14711/thesis-b1155315 ; http://repository.ust.hk/ir/bitstream/1783.1-7346/1/th_redirect.html.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Ren, Lihuan. “GABAA receptor subtype selectivity and structure-activity relationships of flavonoids.” 2011. Web. 02 Mar 2021.
Vancouver:
Ren L. GABAA receptor subtype selectivity and structure-activity relationships of flavonoids. [Internet] [Thesis]. Hong Kong University of Science and Technology; 2011. [cited 2021 Mar 02].
Available from: http://repository.ust.hk/ir/Record/1783.1-7346 ; https://doi.org/10.14711/thesis-b1155315 ; http://repository.ust.hk/ir/bitstream/1783.1-7346/1/th_redirect.html.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Ren L. GABAA receptor subtype selectivity and structure-activity relationships of flavonoids. [Thesis]. Hong Kong University of Science and Technology; 2011. Available from: http://repository.ust.hk/ir/Record/1783.1-7346 ; https://doi.org/10.14711/thesis-b1155315 ; http://repository.ust.hk/ir/bitstream/1783.1-7346/1/th_redirect.html
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of North Carolina – Greensboro
18.
Pyle, Jennifer L.
Identification of agonist and antagonist binding sites at
the G-Protein Coupled Receptor, GPR18.
Degree: 2015, University of North Carolina – Greensboro
URL: http://libres.uncg.edu/ir/listing.aspx?styp=ti&id=19120
► GPR18, a member of the Class A G-Protein Coupled Receptors (GPCRs), is recently a de-orphanized receptor, that upon activation has been found to boost the…
(more)
▼ GPR18, a member of the Class A G-Protein Coupled
Receptors (GPCRs), is recently a de-orphanized receptor, that upon
activation has been found to boost the immune system. Although a
GPR18 crystal structure has not been solved, a homology model of
the inactive state (R) of GPR18 was built in the Reggio lab based
upon the mu-opioid x-ray crystal structure. The present study
continues this work by adding loop regions and N- and C-termini to
the R state model and by developing a model of GPR18 R* (Active)
state, complete with loop regions and N- and C-termini. The
complete inactive and active state models were used for docking
studies of five known GPR18 antagonists,
1,3-dimethoxy-5-methyl-2-[(1R,6R)-3-methyl-6-prop-1-en-2-ylcyclohex-2-en-1-yl]benzene;
1,
(Z)-2-(3((4-chlorobenzyl)oxy)benzylidene)-3-methylene-2,3,6,7-tetrahydro-5H-imidazo[2,1-b][1,3]thiazine;
2,
(1R,2R)-2',6'-dimethoxy-4',5-dimethyl-2-(prop-1-en-2-yl)-1,2,3,4-tetrahydro-1,1'-biphenyl;
3,
(Z)-2-(3-(4-chlorobenzyloxy)benzylidene)-6,7-dihydro-2H-imidazo[2,1-b][1,3]thiazin-3(5H)-one;
4, and
2-[(1R,6R)-6-isopropenyl-3-methylcyclohex-2-en-1-yl]-5-pentylbenzene-1,3-diol;
5 and the GPR18 endogenous ligand, N-arachidonylglycine (NAGly).
Conformational searches were performed on all antagonists using a
systematic search using the Hartree-Fock method at the 6-31G* level
of theory. Because NAGly is highly flexible, its low energy
conformations were determined using the Conformational Memories
method. All low energy conformations, less than 3.0 kcal/mol, of
each ligand were docked using Glide into their respective bundles
based on the state of the receptor each ligand stabilizes. The key
interaction site for all antagonists, an ARG in TMH5, R5.42,
anchors each antagonist in the TMH bundle such that rotameric
changes in key toggle switch residues, F6.48/H6.52, are prevented,
thus preventing the activation of the receptor. The extracellular
loop 2 (EC2 loop) residue Y160 further stabilizes each antagonist
in the binding site. Identified interactions result in Glide scores
consistent with experimental EC50 data. The primary interaction
site for the agonist, NAGly, was TMH2 residue R2.60. An additional
NAGly interaction was identified with the EC2 loop residue K174.
Mutation experiments of key residues identified here are underway
in a collaborator’s lab. These studies will help confirm the
importance of these key residues and further our understanding of
the receptor.; Activation, Binding sites, GPCR, GPR18, Models,
Receptor
Advisors/Committee Members: Patricia Reggio (advisor).
Subjects/Keywords: G proteins – Receptors
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Pyle, J. L. (2015). Identification of agonist and antagonist binding sites at
the G-Protein Coupled Receptor, GPR18. (Masters Thesis). University of North Carolina – Greensboro. Retrieved from http://libres.uncg.edu/ir/listing.aspx?styp=ti&id=19120
Chicago Manual of Style (16th Edition):
Pyle, Jennifer L. “Identification of agonist and antagonist binding sites at
the G-Protein Coupled Receptor, GPR18.” 2015. Masters Thesis, University of North Carolina – Greensboro. Accessed March 02, 2021.
http://libres.uncg.edu/ir/listing.aspx?styp=ti&id=19120.
MLA Handbook (7th Edition):
Pyle, Jennifer L. “Identification of agonist and antagonist binding sites at
the G-Protein Coupled Receptor, GPR18.” 2015. Web. 02 Mar 2021.
Vancouver:
Pyle JL. Identification of agonist and antagonist binding sites at
the G-Protein Coupled Receptor, GPR18. [Internet] [Masters thesis]. University of North Carolina – Greensboro; 2015. [cited 2021 Mar 02].
Available from: http://libres.uncg.edu/ir/listing.aspx?styp=ti&id=19120.
Council of Science Editors:
Pyle JL. Identification of agonist and antagonist binding sites at
the G-Protein Coupled Receptor, GPR18. [Masters Thesis]. University of North Carolina – Greensboro; 2015. Available from: http://libres.uncg.edu/ir/listing.aspx?styp=ti&id=19120

University of Aberdeen
19.
Dorning, Ashley J.
The pharmacology, signalling and expression of the lipid-sensing receptor GPR92.
Degree: PhD, 2013, University of Aberdeen
URL: https://abdn.alma.exlibrisgroup.com/view/delivery/44ABE_INST/12152684420005941
;
https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.582731
► G protein-coupled receptors (GPCRs) are 7 transmembrane domain proteins capable of initiating cellular responses following ligand binding. GPR92 is expressed in the central nervous system…
(more)
▼ G protein-coupled receptors (GPCRs) are 7 transmembrane domain proteins capable of initiating cellular responses following ligand binding. GPR92 is expressed in the central nervous system (CNS) and is demonstrated to be involved in pain signalling via neurons of the spinal cord and dorsal root ganglion (DRG). Activated by the endogenous lipid lysophosphatidic acid (LPA), GPR92 is now considered the 5th LPA receptor. There remains controversy regarding GPR92 pharmacology however, as studies show another endogenous lipid, farnesyl pyrophosphate (FPP), also activates GPR92 with similar potency and efficacy to LPA. LPA-induced activation of GPR92 results in increased intracellular calcium (Ca2+) and cAMP. Furthermore, GPR92 mediates neurite retraction via Rho kinase and activates the cAMP responsive element-binding (CREB) protein. This transcription factor is important in synaptic plasticity and regulates the expression of many neuronal genes including brain derived neurotrophic factor (BDNF), yet the role of GPR92 in the brain has not been explored. Here, I describe the pharmacology and signalling of GPR92, with preliminary data describing its expression. I utilise a cell line lacking endogenous LPA responsiveness (B103 rat neuroblastoma) in which I stably express GPR92. Using intracellular Ca2+ changes and CREB phosphorylation as read-outs, I find FPP to be the most potent and efficacious ligand. The similarly structured lipid geranylgeranyl pyrophosphate (GGPP) produced comparable results to FPP. GPR92-mediated CREB phosphorylation has been described in a mouse model of pain. The ligands which induce this response however, have not been assessed. I describe for the first time, ligand-induced CREB phosphorylation via GPR92, and found that FPP causes the most robust CREB response. LPA and GGPP also induced GPR92-mediated CREB phsphorylation. Furthermore, I find that GPR92 mediates a novel Gq-dependent, Ca2+ independent, signalling pathway resulting in CREB phosphorylation. In addition, I examine GPR92 expression in the CNS using reverse-transcriptase polymerase chain reaction (RT-PCR) and in situ hybridisation. GPR92 is expressed throughout the brain, with particularly high expression in 7 the brain stem, DRG, and hippocampus. In situ hybridisation revealed a distinct expression pattern confirming high levels of GPR92 mRNA in the hippocampal region. GPR92 mRNA is also observed in the cortex, habenula, thalamus and hypothalamus. I also report the expression of the FPP synthesising enzyme FPP synthase (FPPS). Relative expression levels of FPPS between CNS regions are similar to GPR92. Preliminary data suggests FPP is capable of mobilising Ca2+ in hippocampal neuronal and non-neuronal cells. These data suggest an important role for GPR92 in the brain where it, and the enzyme involved in generating one of its endogenous ligands, are highly expressed.
Subjects/Keywords: 610; Cell receptors
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Dorning, A. J. (2013). The pharmacology, signalling and expression of the lipid-sensing receptor GPR92. (Doctoral Dissertation). University of Aberdeen. Retrieved from https://abdn.alma.exlibrisgroup.com/view/delivery/44ABE_INST/12152684420005941 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.582731
Chicago Manual of Style (16th Edition):
Dorning, Ashley J. “The pharmacology, signalling and expression of the lipid-sensing receptor GPR92.” 2013. Doctoral Dissertation, University of Aberdeen. Accessed March 02, 2021.
https://abdn.alma.exlibrisgroup.com/view/delivery/44ABE_INST/12152684420005941 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.582731.
MLA Handbook (7th Edition):
Dorning, Ashley J. “The pharmacology, signalling and expression of the lipid-sensing receptor GPR92.” 2013. Web. 02 Mar 2021.
Vancouver:
Dorning AJ. The pharmacology, signalling and expression of the lipid-sensing receptor GPR92. [Internet] [Doctoral dissertation]. University of Aberdeen; 2013. [cited 2021 Mar 02].
Available from: https://abdn.alma.exlibrisgroup.com/view/delivery/44ABE_INST/12152684420005941 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.582731.
Council of Science Editors:
Dorning AJ. The pharmacology, signalling and expression of the lipid-sensing receptor GPR92. [Doctoral Dissertation]. University of Aberdeen; 2013. Available from: https://abdn.alma.exlibrisgroup.com/view/delivery/44ABE_INST/12152684420005941 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.582731

Rutgers University
20.
Sanner, James William, 1987-.
Identification and characterization of components of rab-6-mediated trafficking in caenorhabditus elegans.
Degree: MS, Microbiology and Molecular Genetics, 2014, Rutgers University
URL: https://rucore.libraries.rutgers.edu/rutgers-lib/45448/
► Membrane trafficking in neurons is an important mechanism used to regulate signaling. Controlling the abundance of AMPA-type receptors at the synapse is important for synaptic…
(more)
▼ Membrane trafficking in neurons is an important mechanism used to regulate signaling. Controlling the abundance of AMPA-type
receptors at the synapse is important for synaptic plasticity and influences processes involved in learning, memory formation, and motor control (Greger and Esteban, 2007; Shepherd and Huganir, 2007). In Caenorhabditis elegans, recycling of the AMPA receptor subunit GLR-1 has been demonstrated to be one method by which synaptic signaling mechanisms can be controlled. Rab GTPases 6.1 and 6.2 have been profiled for their role in trafficking of GLR-1 in neurons. RAB-6.2 plays a role in the retrograde trafficking of the AMPA-type glutamate receptor GLR-1 (Zhang et al., 2012). Activated Rab GTPases regulate membrane trafficking by recruiting multiple effectors, including proteins that modify phospholipid membrane composition, motor proteins that tether membranes to the cytoskeleton, and scaffolding proteins that bind to specific proteins within membranes (Stenmark, 2009). In order to understand RAB-6.2 function, we used a yeast two-hybrid approach to screen for candidate effector molecules. Herein, we discuss the screen performed and detail candidates of interest. We identified one particularly compelling candidate, a phosphoinositol-5-phosphatase named SAC-2, which we hypothesize to be involved in vesicle uncoating and/or in modifying membrane phospholipids. SAC-2::GFP is localized to punctate structures along the ventral nerve cord and in the neuron soma. SAC-2::GFP localization to puncta in the soma is enhanced in rab-6.2(ok2254) null mutant animals, which is opposite to our expectation for RAB-6.2 retrograde cargo or effector molecules. Additionally, we assessed the overlap in functions between RAB-6.1 and RAB-6.2, and delineating their pathways. In agreement with previous data from our lab, our findings suggest that the two RAB-6 isoforms perform similar trafficking functions, but do so independently of each other.
Advisors/Committee Members: Padgett, Richard (chair), Firestein, Bonnie (internal member), Grant, Barth (internal member), Rongo, Christopher (internal member).
Subjects/Keywords: Glutamic acid – Receptors
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Sanner, James William, 1. (2014). Identification and characterization of components of rab-6-mediated trafficking in caenorhabditus elegans. (Masters Thesis). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/45448/
Chicago Manual of Style (16th Edition):
Sanner, James William, 1987-. “Identification and characterization of components of rab-6-mediated trafficking in caenorhabditus elegans.” 2014. Masters Thesis, Rutgers University. Accessed March 02, 2021.
https://rucore.libraries.rutgers.edu/rutgers-lib/45448/.
MLA Handbook (7th Edition):
Sanner, James William, 1987-. “Identification and characterization of components of rab-6-mediated trafficking in caenorhabditus elegans.” 2014. Web. 02 Mar 2021.
Vancouver:
Sanner, James William 1. Identification and characterization of components of rab-6-mediated trafficking in caenorhabditus elegans. [Internet] [Masters thesis]. Rutgers University; 2014. [cited 2021 Mar 02].
Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/45448/.
Council of Science Editors:
Sanner, James William 1. Identification and characterization of components of rab-6-mediated trafficking in caenorhabditus elegans. [Masters Thesis]. Rutgers University; 2014. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/45448/

Rutgers University
21.
Sharp, Liam M., 1989-.
Coarse-grained simulations of nicotinic acetylcholine receptors in complex mixed membranes: embedded lipids and domain partitioning.
Degree: MS, Computational and Integrative Biology, 2016, Rutgers University
URL: https://rucore.libraries.rutgers.edu/rutgers-lib/49832/
► Nicotinic acetylcholine receptors (nAChRs) are pentameric Ligand Gated Ion Channels that are critical to signaling across synapses and the neuromuscular junction; such signaling is facilitated…
(more)
▼ Nicotinic acetylcholine
receptors (nAChRs) are pentameric Ligand Gated Ion Channels that are critical to signaling across synapses and the neuromuscular junction; such signaling is facilitated by high densities of nAChRs in the post-synaptic membrane. Organization of nAChRs, including partitioning behavior in membranes containing dis- tinct lipid domains, is poorly characterized. Numerous experimental studies have shown nAChR gain-of-function likely caused by direct interactions with cholesterol, but a significant role for lipid domains has been suggested by nAChR gain-of-function upon bulk cholesterol depletion. Furthermore, the opportunity for cholesterol to have a direct interactions will likely have a complex dependence on the extent of domain formation and lipid species in the membrane, which has not been previously addressed. In the present research, we use Molecular Dynamics Simulations with coarse-grained resolution via the MARTINI model to investigate concentrations of cholesterol and other lipids local to nAChRs embedded in complex model membranes with a range of head groups and degrees of unsaturation. Cholesterol and unsaturated lipids are observed binding in deep non-annular sites in the nAChR bundle (based on the 2BG9 cryo-EM structure), consistent with our previous predictions. nAChR partitions, however, into cholesterol-poor phases, resulting in dynamic exchange between cholesterol and unsaturated phospholipids.
Advisors/Committee Members: Brannigan, Grace (chair), Martin, Joseph (internal member), O'Malley, Sean (internal member).
Subjects/Keywords: Lipids; Nicotinic receptors
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Sharp, Liam M., 1. (2016). Coarse-grained simulations of nicotinic acetylcholine receptors in complex mixed membranes: embedded lipids and domain partitioning. (Masters Thesis). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/49832/
Chicago Manual of Style (16th Edition):
Sharp, Liam M., 1989-. “Coarse-grained simulations of nicotinic acetylcholine receptors in complex mixed membranes: embedded lipids and domain partitioning.” 2016. Masters Thesis, Rutgers University. Accessed March 02, 2021.
https://rucore.libraries.rutgers.edu/rutgers-lib/49832/.
MLA Handbook (7th Edition):
Sharp, Liam M., 1989-. “Coarse-grained simulations of nicotinic acetylcholine receptors in complex mixed membranes: embedded lipids and domain partitioning.” 2016. Web. 02 Mar 2021.
Vancouver:
Sharp, Liam M. 1. Coarse-grained simulations of nicotinic acetylcholine receptors in complex mixed membranes: embedded lipids and domain partitioning. [Internet] [Masters thesis]. Rutgers University; 2016. [cited 2021 Mar 02].
Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/49832/.
Council of Science Editors:
Sharp, Liam M. 1. Coarse-grained simulations of nicotinic acetylcholine receptors in complex mixed membranes: embedded lipids and domain partitioning. [Masters Thesis]. Rutgers University; 2016. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/49832/
22.
Court Lecuyer, Nathalie.
Intéractions hôte-pathogène : rôle des pattern recognition receptors (PRR) dans l'induction de la réponse immunitaire aux mycobactéries et modulation de cette réponse par des composés mycobactériens : Host-pathogen interactions : role of pattern recognition receptors (PRR) for the induction of the host immune response in response to mycobacteria and modulation of this response by mycobacterial components.
Degree: Docteur es, Biologie cellulaire et moléculaire. Immunologie, 2010, Université d'Orléans
URL: http://www.theses.fr/2010ORLE2087
► Nos travaux ont permis d’étudier différents aspects des interactions hôte-pathogènes. L’étude de différents Pattern Recognition Receptors (PRR) autres que les TLR, ainsi que leurs associations…
(more)
▼ Nos travaux ont permis d’étudier différents aspects des interactions hôte-pathogènes. L’étude de différents Pattern Recognition Receptors (PRR) autres que les TLR, ainsi que leurs associations a mis en évidence une redondance partielle entre les récepteurs des familles des Scavenger Receptors, lectines de type C et EMR1 in vitro et in vivo dans l’induction de la réponse immunitaire à Mycobacterium tuberculosis. Cette compensation entre les récepteurs contraste avec les rôles indépendants et non redondants des cytokines et de leurs voies associées comme le TNF, l’IL-1R1, L’IFNγR et MyD88, indispensables pour le contrôle de l’infection. Grâce à l’utilisation de nouvelles souris génétiquement modifiées, nous avons pu montrer un rôle minime de la lymphotoxine α dans le contrôle de l’infection par Mycobacterium tuberculosis contrairement au rôle primordial du TNF. Enfin, notre étude s’est poursuivie avec l’analyse de la modulation de la réponse immunitaire de l’hôte par des composants de la paroi des mycobactéries, les PIM. L’élaboration de PIM synthétiques a permis de montrer que ces molécules de faibles poids moléculaires inhibent l’induction des voies TLR4 et TLR2 et possèdent ainsi un potentiel anti-inflammatoire thérapeutique.
In these study, we aimed to investigate different aspects of the host-pathogen interactions. We investigated the involvement of various Pattern Recognition Receptors (PRR) other than TLR, and their associations for the control of M. tuberculosis infection. We highlighted a partial redundancy between members of the Scavenger Receptors family, C-type lectins and EMR1 in response to mycobacteria in vitro and in vivo. This is in sharp contrast with the cytokine pathways like TNF, IL-1R1, IFNγR and MyD88, essentials to control M. tuberculosis infection and which cannot compensate with each other. By using new genetically deficient mice, we showed a limited role for the lymphotoxin α in the control of the infection by Mycobacterium tuberculosis in contrast with the vital role for TNF. Finally, we analysed the modulation of the immune response by mycobacterial cell wall components, PIM. Use of synthetic PIM demonstrated that these small molecules exert an inhibitory activity on TLR4- and TLR2-signaling pathways and may have a therapeutic anti-inflammatory potential.
Advisors/Committee Members: Quesniaux, Valérie (thesis director), Ryffel, Bernhard (thesis director).
Subjects/Keywords: PRR; Scavenger receptors; EMR1; PIM; Pattern Recognition Receptors; Scavenger receptors; EMR1; PIM
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
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APA (6th Edition):
Court Lecuyer, N. (2010). Intéractions hôte-pathogène : rôle des pattern recognition receptors (PRR) dans l'induction de la réponse immunitaire aux mycobactéries et modulation de cette réponse par des composés mycobactériens : Host-pathogen interactions : role of pattern recognition receptors (PRR) for the induction of the host immune response in response to mycobacteria and modulation of this response by mycobacterial components. (Doctoral Dissertation). Université d'Orléans. Retrieved from http://www.theses.fr/2010ORLE2087
Chicago Manual of Style (16th Edition):
Court Lecuyer, Nathalie. “Intéractions hôte-pathogène : rôle des pattern recognition receptors (PRR) dans l'induction de la réponse immunitaire aux mycobactéries et modulation de cette réponse par des composés mycobactériens : Host-pathogen interactions : role of pattern recognition receptors (PRR) for the induction of the host immune response in response to mycobacteria and modulation of this response by mycobacterial components.” 2010. Doctoral Dissertation, Université d'Orléans. Accessed March 02, 2021.
http://www.theses.fr/2010ORLE2087.
MLA Handbook (7th Edition):
Court Lecuyer, Nathalie. “Intéractions hôte-pathogène : rôle des pattern recognition receptors (PRR) dans l'induction de la réponse immunitaire aux mycobactéries et modulation de cette réponse par des composés mycobactériens : Host-pathogen interactions : role of pattern recognition receptors (PRR) for the induction of the host immune response in response to mycobacteria and modulation of this response by mycobacterial components.” 2010. Web. 02 Mar 2021.
Vancouver:
Court Lecuyer N. Intéractions hôte-pathogène : rôle des pattern recognition receptors (PRR) dans l'induction de la réponse immunitaire aux mycobactéries et modulation de cette réponse par des composés mycobactériens : Host-pathogen interactions : role of pattern recognition receptors (PRR) for the induction of the host immune response in response to mycobacteria and modulation of this response by mycobacterial components. [Internet] [Doctoral dissertation]. Université d'Orléans; 2010. [cited 2021 Mar 02].
Available from: http://www.theses.fr/2010ORLE2087.
Council of Science Editors:
Court Lecuyer N. Intéractions hôte-pathogène : rôle des pattern recognition receptors (PRR) dans l'induction de la réponse immunitaire aux mycobactéries et modulation de cette réponse par des composés mycobactériens : Host-pathogen interactions : role of pattern recognition receptors (PRR) for the induction of the host immune response in response to mycobacteria and modulation of this response by mycobacterial components. [Doctoral Dissertation]. Université d'Orléans; 2010. Available from: http://www.theses.fr/2010ORLE2087

University of Arizona
23.
Hild-Petito, Sheri Ann.
Distribution of estrogen and progesterone receptors in the primate ovary, with emphasis on subpopulations of cells within the corpus luteum.
Degree: 1988, University of Arizona
URL: http://hdl.handle.net/10150/184485
► Both estradiol and progeterone are proposed autocrine or paracrine regulators of ovarian function in primate species. However, specific receptors for these steroids have not been…
(more)
▼ Both estradiol and progeterone are proposed autocrine or paracrine regulators of ovarian function in primate species. However, specific receptors for these steroids have not been localized to individual compartments of the primate ovary. Using immunocytochemical techniques, estradiol receptors were detected in the germinal epithelium, but not other structures, of ovaries obtained from rhesus or cynomolgus monkeys during the follicular and luteal phases of the menstrual cycle. In contrast, progesterone receptors were present in stromal and interstitial tissue, the thecal layers of healthy and atretic follicles, as well as the functional corpus luteum. These results are consistent with the concept of a receptor-mediated role for progesterone, but not estrogen, within the predominant gametogenic and endocrine structures, e.g., the follicle and corpus luteum, of the primate ovary. The recent discovery of distinct cell types in the corpus luteum of domestic ungulates has revised concepts on the control of luteal function in these species. Studies were designed to test the hypothesis that the primate corpus luteum consists of cell subpopulations that differ in physical characteristics, function and regulation. Cells enzymatically-dispersed from the monkey corpus luteum at mid-luteal phase of the menstrual cycle differed in size (diameter) and the presence of the steroidogenic enzyme, 3β-hydroxysteroid dehydrogenase (3β-HSD). Analysis of dispersed cells for forward and 90° light scatter properties by flow cytometry revealed two distinct continua (Cα and Cβ). These continua were isolated using the sorting capabilities of the flow cytometer. Cα contained single cells of ≤ 15 μm and cell clusters; the cells were typically 3β-HSD-negative nonsteroidogenic. Cβ consisted of single cells that increased in size up to 40 μm and were 3β-HSD-positive. Cβ was divided into two regions (R₁ and R₃) and the cells isolated. R₁ cells were ≤ 15 μm whereas R₃ cells were ≥ 20 μm. Basal progesterone and estrogen production by R₃ cells was greater than that produced by R₁ cells (as determined by radioimmunoassay of the incubation media). Relative stimulation of progesterone production by hCG, cAMP or PGE₂ was not different between R₁ and R₃ luteal cells. These results support the hypothesis that the primate corpus luteum consists of distinct cell subpopulations which differ in size and steroidogenic capacity. However, the cell types which secrete progesterone are typically responsive to gonadotropin and PGE₂, possibly via a cAMP-mediated pathway.
Subjects/Keywords: Estrogen – Receptors.;
Progesterone – Receptors.;
Hormone receptors.
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Chicago ·
MLA ·
Vancouver ·
CSE |
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APA (6th Edition):
Hild-Petito, S. A. (1988). Distribution of estrogen and progesterone receptors in the primate ovary, with emphasis on subpopulations of cells within the corpus luteum.
(Doctoral Dissertation). University of Arizona. Retrieved from http://hdl.handle.net/10150/184485
Chicago Manual of Style (16th Edition):
Hild-Petito, Sheri Ann. “Distribution of estrogen and progesterone receptors in the primate ovary, with emphasis on subpopulations of cells within the corpus luteum.
” 1988. Doctoral Dissertation, University of Arizona. Accessed March 02, 2021.
http://hdl.handle.net/10150/184485.
MLA Handbook (7th Edition):
Hild-Petito, Sheri Ann. “Distribution of estrogen and progesterone receptors in the primate ovary, with emphasis on subpopulations of cells within the corpus luteum.
” 1988. Web. 02 Mar 2021.
Vancouver:
Hild-Petito SA. Distribution of estrogen and progesterone receptors in the primate ovary, with emphasis on subpopulations of cells within the corpus luteum.
[Internet] [Doctoral dissertation]. University of Arizona; 1988. [cited 2021 Mar 02].
Available from: http://hdl.handle.net/10150/184485.
Council of Science Editors:
Hild-Petito SA. Distribution of estrogen and progesterone receptors in the primate ovary, with emphasis on subpopulations of cells within the corpus luteum.
[Doctoral Dissertation]. University of Arizona; 1988. Available from: http://hdl.handle.net/10150/184485

Oregon State University
24.
Das, Siba Ranjan.
Influence of aging and behavioral experience on expression of GluN1 splice variants of the NMDA receptor in prefrontal cortex of mice brain.
Degree: PhD, Molecular and Cellular Biology, 2010, Oregon State University
URL: http://hdl.handle.net/1957/18854
► As the aging population continues to grow the world over, age related complications become more and more apparent among the elderly population. One such complication…
(more)
▼ As the aging population continues to grow the world over, age related complications become more and more apparent among the elderly population. One such complication is age associated memory impairment, which makes the elderly more dependent on caregivers early on. NMDA
receptors in the brain are important for memory formation, consolidation and retrieval. Expression of NMDA
receptors declines with age, which is associated with declines in memory observed during aging. Age-related changes in the protein and mRNA expression of some of the splice forms of the GluN1 (GluN1, NR1) subunit of the NMDA receptor have been seen in mice and rats. The present study was designed to determine whether individual splice forms of the GluN1 subunit of the NMDA receptor within prefrontal / frontal cortical regions contribute to memory deficits during aging and whether experience in learning tasks can influence the expression of the splice forms.
mRNA expression of 4 splice forms GluN1[subscript X11], GluN1[subscript X10], GluN1[subscript 0XX] and GluN1[subscript 1XX] (GluN1-1, GluN1-3, GluN1-a and GluN1-b, respectively) and mRNA for all known splice forms (GluN1-pan) were examined by in situ hybridization. mRNA for the C-terminal splice forms, GluN1[subscript X11] (GluN1-1; +C1 and +C2 cassettes) and GluN1[subscript X10] (GluN1-3; +C1 and +C2'), showed significant declines during aging in several brain regions even though overall GluN1-pan mRNA expression was not significantly affected by aging. This work provides evidence that these splice forms are more influenced by aging than the subunit as a whole. There was an increase in the expression of GluN1[subscript 0XX] (GluN1-a; -N1 cassette) splice form in the behaviorally-experienced old mice relative to the younger groups. Old mice with the highest levels of mRNA expression for the GluN1[subscript 0XX] (GluN1-a) splice form in orbital cortex showed the best performances in spatial working and reference memory tasks, but the poorest performances in a cued, associative learning task. These results suggest that the GluN1[subscript 0XX] subunit splice variant may be important for spatial memory performance in the old animals.
Protein expression of GluN1 subunits containing C-terminal cassettes C2 or C2' were observed to decline with increasing age, regardless of experience. In middle-age animals, higher expressions of the GluN1 subunit and C2' cassette proteins were associated with good reference memory on initial search. In the aged animals, higher protein expression of GluN1 subunits containing C1 cassettes and the whole population of GluN1 subunits were found to be associated with better performance in the final phase of probe trials but this appeared to be due to perseveration or delays in applying an accurate search. These results provide support for the theory that there is heterogeneity in the effect of aging on the expression of the GluN1 subunits containing different splice cassettes. It also suggests that the GluN1 subunit might be most important for good reference…
Advisors/Committee Members: Magnusson, Kathy R. (advisor), Hagen, Tory (committee member).
Subjects/Keywords: NMDA receptor; Methyl aspartate – Receptors
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Das, S. R. (2010). Influence of aging and behavioral experience on expression of GluN1 splice variants of the NMDA receptor in prefrontal cortex of mice brain. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/18854
Chicago Manual of Style (16th Edition):
Das, Siba Ranjan. “Influence of aging and behavioral experience on expression of GluN1 splice variants of the NMDA receptor in prefrontal cortex of mice brain.” 2010. Doctoral Dissertation, Oregon State University. Accessed March 02, 2021.
http://hdl.handle.net/1957/18854.
MLA Handbook (7th Edition):
Das, Siba Ranjan. “Influence of aging and behavioral experience on expression of GluN1 splice variants of the NMDA receptor in prefrontal cortex of mice brain.” 2010. Web. 02 Mar 2021.
Vancouver:
Das SR. Influence of aging and behavioral experience on expression of GluN1 splice variants of the NMDA receptor in prefrontal cortex of mice brain. [Internet] [Doctoral dissertation]. Oregon State University; 2010. [cited 2021 Mar 02].
Available from: http://hdl.handle.net/1957/18854.
Council of Science Editors:
Das SR. Influence of aging and behavioral experience on expression of GluN1 splice variants of the NMDA receptor in prefrontal cortex of mice brain. [Doctoral Dissertation]. Oregon State University; 2010. Available from: http://hdl.handle.net/1957/18854

Universiteit Utrecht
25.
Bink, D.I.
Kainate receptors and cognitive enhancement.
Degree: 2011, Universiteit Utrecht
URL: http://dspace.library.uu.nl:8080/handle/1874/193556
► The kainate (KA) receptor family is one of the three ionotropic glutamate receptors (iGluRs) families, consisting of five subunits. The receptors were first discovered by…
(more)
▼ The kainate (KA) receptor family is one of the three ionotropic glutamate
receptors (iGluRs) families, consisting of five subunits. The
receptors were first discovered by the excitotoxic and epileptogenic actions upon kainate injection. The KA
receptors are located on the nerve terminals as well as on the dendrites of neurons and induce ionotropic and metabotropic signalling pathways in areas like the hippocampus, amygdala, cerebellum and the cerebral cortex. Kainate
receptors play a role in short-term and long-term synaptic facilitation and plasticity and are thereby a potential target for enhancing cognition. However, most of the actions of KA
receptors are bidirectional and are highly dependent on experimental conditions, making them difficult subjects to study and target. This review describes some of the properties of these
receptors and attempts to summarize the findings on cognitive modulation by KA
receptors reported till now.
Advisors/Committee Members: Oosting, R.S..
Subjects/Keywords: Kainate receptors; Glutamate; LTP; Cognition
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MLA ·
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CSE |
Export
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APA (6th Edition):
Bink, D. I. (2011). Kainate receptors and cognitive enhancement. (Masters Thesis). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/193556
Chicago Manual of Style (16th Edition):
Bink, D I. “Kainate receptors and cognitive enhancement.” 2011. Masters Thesis, Universiteit Utrecht. Accessed March 02, 2021.
http://dspace.library.uu.nl:8080/handle/1874/193556.
MLA Handbook (7th Edition):
Bink, D I. “Kainate receptors and cognitive enhancement.” 2011. Web. 02 Mar 2021.
Vancouver:
Bink DI. Kainate receptors and cognitive enhancement. [Internet] [Masters thesis]. Universiteit Utrecht; 2011. [cited 2021 Mar 02].
Available from: http://dspace.library.uu.nl:8080/handle/1874/193556.
Council of Science Editors:
Bink DI. Kainate receptors and cognitive enhancement. [Masters Thesis]. Universiteit Utrecht; 2011. Available from: http://dspace.library.uu.nl:8080/handle/1874/193556

University of Utah
26.
Sonsalla, Patricia Kay.
The Roles of D1 and D2 dopamine receptors in mediating methamphetamine-induced changes in monoamine and substance P systems.
Degree: PhD, Pharmacology & Toxicology;, 1985, University of Utah
URL: http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/301/rec/1225
► The amphetamine-like compounds have prominent effects on several neurotransmitter systems within the central nervous system. The administration of multiple doses of these stimulants produces profound…
(more)
▼ The amphetamine-like compounds have prominent effects on several neurotransmitter systems within the central nervous system. The administration of multiple doses of these stimulants produces profound and long-lasting decreases in neurochemical indices of dopamine and serotonergic systems as well as increases in the concentration of the neuropeptide, substance P, within various brain regions. The nonselective dopamine receptor antagonist, haloperidol, blocks these effects of methamphetamine on all three neurotransmitter systems. The purpose of the present investigations was to identify the dopamine receptor subtype(s) which mediate these actions of methamphetamine. Thus, selective D1 and D2 agonists and antagonists were administered alone or in combination with methamphetamine and the effects of these treatments on neurochemical parameters of the dopamine, serotonin, and substance P systems were evaluated. The results from these present studies suggest that methamphetamine-released dopamine actions on the D2 receptor mediate the effects of methamphetamine on the nigrostriatal dopamine and striatonigral substance P systems. In contrast, the effects of methamphetamine on serotonin systems appear to involve dopamine actions on the D1 receptor. A single administration of an amphetamine produces a rapid, but transient depression of serotonin synthesis. The coadministration of dopamine antagonists with methamphetamine did not modify the methamphetamine effect on tryptophan hydroxylase activity, the rate-limiting enzyme in serotonin synthesis. These findings suggest a different mechanism is involved in this acute, transient effect of methamphetamine versus the long-lasting effects observed after multiple administrations. Whereas D1 blockade attenuates the effects of methamphetamine on the serotonergic system in the multiple dosing paradigm, it does not afford protection against the acute effect.
Subjects/Keywords: Receptors; Dopamine
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APA ·
Chicago ·
MLA ·
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Export
to Zotero / EndNote / Reference
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APA (6th Edition):
Sonsalla, P. K. (1985). The Roles of D1 and D2 dopamine receptors in mediating methamphetamine-induced changes in monoamine and substance P systems. (Doctoral Dissertation). University of Utah. Retrieved from http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/301/rec/1225
Chicago Manual of Style (16th Edition):
Sonsalla, Patricia Kay. “The Roles of D1 and D2 dopamine receptors in mediating methamphetamine-induced changes in monoamine and substance P systems.” 1985. Doctoral Dissertation, University of Utah. Accessed March 02, 2021.
http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/301/rec/1225.
MLA Handbook (7th Edition):
Sonsalla, Patricia Kay. “The Roles of D1 and D2 dopamine receptors in mediating methamphetamine-induced changes in monoamine and substance P systems.” 1985. Web. 02 Mar 2021.
Vancouver:
Sonsalla PK. The Roles of D1 and D2 dopamine receptors in mediating methamphetamine-induced changes in monoamine and substance P systems. [Internet] [Doctoral dissertation]. University of Utah; 1985. [cited 2021 Mar 02].
Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/301/rec/1225.
Council of Science Editors:
Sonsalla PK. The Roles of D1 and D2 dopamine receptors in mediating methamphetamine-induced changes in monoamine and substance P systems. [Doctoral Dissertation]. University of Utah; 1985. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/301/rec/1225

University of Utah
27.
Lin, Ching-Shan.
Pharmacokinetic study of uterine progesterone retention;.
Degree: PhD, Pharmaceutics & Pharmaceutical Chemistry;, 1997, University of Utah
URL: http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/1012/rec/971
► Tritium-labeled progesterone was administered to mature female rate in the proestrous stage of three different routes, gastric intubation, subcutaneous injection, and uterine intraluminal instillation, to…
(more)
▼ Tritium-labeled progesterone was administered to mature female rate in the proestrous stage of three different routes, gastric intubation, subcutaneous injection, and uterine intraluminal instillation, to study the kinetics involved in the uptake and retention of radioactivity by the uterus and various other tissues. Progesterone was retained at a much higher level and for a more prolonged period in the rat uterus after uterine intraluminal instillation. Progesterone bioavailability to the uterus was 45 times higher by uterine intraluminal instillation than by either gastric intubation or subcutaneous injection. Progesterone absorption by the rat endometrium was extremely fast. The observed biphasic decrease of radioactivity from the uterine tissue was explained adequately by a pharmacokinetic model in which progesterone is assumed to be present in two compartments within the uterine tissue. The pharmacokinetic parameters showed that the progesterone biological half-life in the uterine tissue during the alpha-phase was about 6.5 min, while that in the beta-phase was about 230 min. Tritium-labeled progesterone was perfused through the uterine luminal cavity of the rat until a steady state was reached. The radioactivity was found to exist at a mush higher concentration in the uterine tissue than many other tissues studies. The amount of radioactivity retained by the uterine tissue under constant perfusion was adequately described by a pharmacokinetic equation derived from a two-compartment open model. The amount of progesterone residing in the uterine tissue may, thus, be predicted from this equation using the predetermined pharmacokinetic parameters. At the steady state, the observed experimental values were in close approximation with the predicted values. A cyclic change in the uptake and retention of progesterone by uterine tissues was observed with rats in different stages of estrous cycle. Long-term insertion of the “Uterine Progesterone System,” however, did not cause any significant change in the uterine capacity for either the absorption or the retention of progesterone administered by the direct uterine intraluminal route. The levels of the nuclear receptors for estradiol-17beta and progesterone in the uterine tissues of rats were measured by a 3H-steroid exchange method in which the specific 3H-steroid binding sites were estimated after the addition of an excess amount of the same but nonlabeled steroid. A similar procedure was used for the quantification of the progesterone cytosol receptor in rat uterine tissue. The level of the uterine estradiol-17beta cytosol receptor was determined from the amount of 8S complex obtained after a sucrose gradient centrifugation. The highest amounts of estradiol-17beta and progesterone receptors were found in uterine nuclei and cytosol of animals in proestrous, followed by diestrus, estrus, and metestrus in that order. No significant changes in the levels of either nuclear or cytosol receptors for both hormones were observed in the uterine tissues of rats inserted “Uterine…
Subjects/Keywords: Pharmacokinetics; Receptors
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APA ·
Chicago ·
MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Lin, C. (1997). Pharmacokinetic study of uterine progesterone retention;. (Doctoral Dissertation). University of Utah. Retrieved from http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/1012/rec/971
Chicago Manual of Style (16th Edition):
Lin, Ching-Shan. “Pharmacokinetic study of uterine progesterone retention;.” 1997. Doctoral Dissertation, University of Utah. Accessed March 02, 2021.
http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/1012/rec/971.
MLA Handbook (7th Edition):
Lin, Ching-Shan. “Pharmacokinetic study of uterine progesterone retention;.” 1997. Web. 02 Mar 2021.
Vancouver:
Lin C. Pharmacokinetic study of uterine progesterone retention;. [Internet] [Doctoral dissertation]. University of Utah; 1997. [cited 2021 Mar 02].
Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/1012/rec/971.
Council of Science Editors:
Lin C. Pharmacokinetic study of uterine progesterone retention;. [Doctoral Dissertation]. University of Utah; 1997. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/1012/rec/971
28.
Mailhac, Camille.
Développement d’outils pour l'étude de la signalisation médiée par les récepteurs couplés aux protéines G, basés sur l'utilisation d'anticorps à domaine unique de lama : Development of tools for the study of G protein-coupled receptor-mediated signaling based on the use of lama single domain antibodies.
Degree: Docteur es, Biologie. Immunologie, 2017, Aix Marseille Université
URL: http://www.theses.fr/2017AIXM0405
► L'objectif principal de ma thèse était de développer de nouvelles technologies et des outils pour l’étude de l’activation des récepteurs couplés aux protéines G (GPCR).À…
(more)
▼ L'objectif principal de ma thèse était de développer de nouvelles technologies et des outils pour l’étude de l’activation des récepteurs couplés aux protéines G (GPCR).À la surface de la cellule se trouve une multitude de récepteurs qui jouent un rôle critique dans la communication cellule-cellule, dont les GPCR, une famille de récepteur utilisant les protéines G intracellulaires pour transmettre leurs signaux. Le ciblage de ces récepteurs à des fins thérapeutique est innovant et très prometteur. Mais à ce jour seuls quelques médicaments ciblant les GPCR ont été mis sur le marché, en partie en raison d'un manque d'outils permettant le suivi de leur action sur les cellules natives.L’objectif de cette thèse est donc de développer des tests simples pour suivre l’activation de n’importe quel GPCR. Pour développer ce type de test, nous avons décidé d'utiliser des fragments d'anticorps appelés nanobodies. Les anticorps sont des protéines du sang produites en réponse à un antigène spécifique qui sont capable de le neutraliser. Les nanobodies correspondent au domaine variable de certains anticorps de camélidés. En raison de leur faible taille (13 kDa) et de leur site de liaison à l'antigène réduit, les nanobodies se lient souvent à des cavités et présentent une grande sensibilité aux changements de conformation de l'antigène.
The main objective of my thesis was to develop technologies and tools to study activation of G protein-coupled receptors (GPCRs).The cell surface is displaying a multitude of receptors, who play critical roles in cell-cell communication. Among them, GPCRs represent a large family relying on the use of intracellular G proteins for their signaling. Targeting these receptors for therapies is very promising and innovative. So far, only few new drugs have been put on the market, partly due to a lack of tools enabling the follow-up of their action on native cells.The aim of this thesis is thus to develop simple assays to study activation of any GPCRs. To develop this kind of test, we used antibody fragments called nanobodies. Antibodies are blood protein produced in response to and counteracting a specific antigen. Nanobodies correspond to antibody fragments derived from the variable domain of a special class of camelid antibodies. Because of their small size (13 kDa) and reduced antigen binding site, nanobodies often bind cavities and show a high sensitivity to antigen conformational changes.
Advisors/Committee Members: Chames, Patrick (thesis director).
Subjects/Keywords: RCPG; G protein coupled receptors
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
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to Zotero / EndNote / Reference
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APA (6th Edition):
Mailhac, C. (2017). Développement d’outils pour l'étude de la signalisation médiée par les récepteurs couplés aux protéines G, basés sur l'utilisation d'anticorps à domaine unique de lama : Development of tools for the study of G protein-coupled receptor-mediated signaling based on the use of lama single domain antibodies. (Doctoral Dissertation). Aix Marseille Université. Retrieved from http://www.theses.fr/2017AIXM0405
Chicago Manual of Style (16th Edition):
Mailhac, Camille. “Développement d’outils pour l'étude de la signalisation médiée par les récepteurs couplés aux protéines G, basés sur l'utilisation d'anticorps à domaine unique de lama : Development of tools for the study of G protein-coupled receptor-mediated signaling based on the use of lama single domain antibodies.” 2017. Doctoral Dissertation, Aix Marseille Université. Accessed March 02, 2021.
http://www.theses.fr/2017AIXM0405.
MLA Handbook (7th Edition):
Mailhac, Camille. “Développement d’outils pour l'étude de la signalisation médiée par les récepteurs couplés aux protéines G, basés sur l'utilisation d'anticorps à domaine unique de lama : Development of tools for the study of G protein-coupled receptor-mediated signaling based on the use of lama single domain antibodies.” 2017. Web. 02 Mar 2021.
Vancouver:
Mailhac C. Développement d’outils pour l'étude de la signalisation médiée par les récepteurs couplés aux protéines G, basés sur l'utilisation d'anticorps à domaine unique de lama : Development of tools for the study of G protein-coupled receptor-mediated signaling based on the use of lama single domain antibodies. [Internet] [Doctoral dissertation]. Aix Marseille Université 2017. [cited 2021 Mar 02].
Available from: http://www.theses.fr/2017AIXM0405.
Council of Science Editors:
Mailhac C. Développement d’outils pour l'étude de la signalisation médiée par les récepteurs couplés aux protéines G, basés sur l'utilisation d'anticorps à domaine unique de lama : Development of tools for the study of G protein-coupled receptor-mediated signaling based on the use of lama single domain antibodies. [Doctoral Dissertation]. Aix Marseille Université 2017. Available from: http://www.theses.fr/2017AIXM0405

University of Hong Kong
29.
Lui, Wan, Thomas.
Biochemical events induced
by the specific kappa-opioid receptor agonist, U50488H.
Degree: 2007, University of Hong Kong
URL: http://hdl.handle.net/10722/131320
Subjects/Keywords: Opioids
- Receptors.
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Lui, Wan, T. (2007). Biochemical events induced
by the specific kappa-opioid receptor agonist, U50488H. (Thesis). University of Hong Kong. Retrieved from http://hdl.handle.net/10722/131320
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Lui, Wan, Thomas. “Biochemical events induced
by the specific kappa-opioid receptor agonist, U50488H.” 2007. Thesis, University of Hong Kong. Accessed March 02, 2021.
http://hdl.handle.net/10722/131320.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Lui, Wan, Thomas. “Biochemical events induced
by the specific kappa-opioid receptor agonist, U50488H.” 2007. Web. 02 Mar 2021.
Vancouver:
Lui, Wan T. Biochemical events induced
by the specific kappa-opioid receptor agonist, U50488H. [Internet] [Thesis]. University of Hong Kong; 2007. [cited 2021 Mar 02].
Available from: http://hdl.handle.net/10722/131320.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Lui, Wan T. Biochemical events induced
by the specific kappa-opioid receptor agonist, U50488H. [Thesis]. University of Hong Kong; 2007. Available from: http://hdl.handle.net/10722/131320
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Rochester
30.
Saperstein, Sara C.
Modulation of TNF-α-mediated acute lung inflammation : a
role for IL-1β and soluble TNF receptors.
Degree: PhD, 2009, University of Rochester
URL: http://hdl.handle.net/1802/8631
► Tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) are key mediators of numerous lung inflammatory diseases. Induced by similar stimuli, in many cell types IL-1β modifies…
(more)
▼ Tumor necrosis factor-α (TNF-α) and interleukin-1β
(IL-1β) are key mediators of numerous lung inflammatory diseases.
Induced by similar stimuli, in many cell types IL-1β modifies
expression and solubilization of the two TNF receptors, TNFR1 and
TNFR2. These proteins are cleaved from the cell surface primarily
by the metalloprotease TNF-α converting enzyme (TACE) generating
soluble forms still capable of binding TNF-α. Investigators
postulate that the shed receptors either act as natural antagonists
or as a TNF-α reservoir extending the half-life of the cognate
ligand. Based on these observations, IL-1β effects on lung
TNF-α-mediated inflammatory responses, as well as on TNF receptor
expression and shedding, both in vitro and in vivo were
investigated. Finally, the roles of the two soluble TNF receptors
(sTNFR) on altering ligand-induced inflammation and cytotoxicity
were analyzed. Studies utilizing an in vitro culture system, murine
epithelial type II-like cells (MLE-15), and transgenic IL-1β null
mice showed that the interleukin enhances acute, TNF-α-mediated
pulmonary inflammatory processes. In vitro, IL-1β induced MLE-15
surface TNF receptor expression which correlated with increased
mRNA levels and protein secretion of the two neutrophil
chemoattractants macrophage inflammatory protein (MIP-2) and KC
following TNF-α exposure. Further studies investigating the
regulation of the two receptors demonstrated a counter-regulatory
role for each; TNFR1 expression was necessary for
interleukin-mediated TNFR2 shedding and surface expression and vice
a versa. In vivo studies corroborated these findings. IL-1β KO mice
instilled with TNF-α had decreased neutrophil influx measured in
the lavage fluid of the lung. This correlated with a decrease in
lavage sTNFR1 and MIP-2, although sTNFR2 and KC were unaffected.
Finally, at a ratio of approximately 1:1 to 1:25, sTNFR1:sTNFR2
functioned as TNF-α antagonists in vitro. Taken together, the
studies in this thesis suggest that IL-1β modulates TNF-α-mediated
inflammatory lung diseases by altering TNF receptor shedding and
expression in epithelial cells, resulting in altered
chemoattractant production in response to TNF-α, hence augmenting
recruitment of inflammatory cells.
Subjects/Keywords: Inflammation; Receptors
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Saperstein, S. C. (2009). Modulation of TNF-α-mediated acute lung inflammation : a
role for IL-1β and soluble TNF receptors. (Doctoral Dissertation). University of Rochester. Retrieved from http://hdl.handle.net/1802/8631
Chicago Manual of Style (16th Edition):
Saperstein, Sara C. “Modulation of TNF-α-mediated acute lung inflammation : a
role for IL-1β and soluble TNF receptors.” 2009. Doctoral Dissertation, University of Rochester. Accessed March 02, 2021.
http://hdl.handle.net/1802/8631.
MLA Handbook (7th Edition):
Saperstein, Sara C. “Modulation of TNF-α-mediated acute lung inflammation : a
role for IL-1β and soluble TNF receptors.” 2009. Web. 02 Mar 2021.
Vancouver:
Saperstein SC. Modulation of TNF-α-mediated acute lung inflammation : a
role for IL-1β and soluble TNF receptors. [Internet] [Doctoral dissertation]. University of Rochester; 2009. [cited 2021 Mar 02].
Available from: http://hdl.handle.net/1802/8631.
Council of Science Editors:
Saperstein SC. Modulation of TNF-α-mediated acute lung inflammation : a
role for IL-1β and soluble TNF receptors. [Doctoral Dissertation]. University of Rochester; 2009. Available from: http://hdl.handle.net/1802/8631
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