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You searched for subject:(Reb1). Showing records 1 – 2 of 2 total matches.

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Louisiana State University

1. Wang, Qing. Regulatory Impacts of Assembled Pol III Complexes on Neighboring Pol II Transcribed Genes in Saccharomyces cerevisiae.

Degree: PhD, 2015, Louisiana State University

RNA polymerase III (Pol III) transcribes tRNAs as well as other small non-coding RNAs. tRNA genes contain internal promoter sequences (A- and B-boxes) which can be specifically recognized and bound by TFIIIC. Binding of TFIIIC facilitates TFIIIB recruitment, which in turn targets Pol III to be recruited and initiate transcription. In addition to typical tRNA genes, there are other chromosomal regions, referred as extra-TFIIIC (ETC) sites, that are only bound by TFIIIC. Apart from transcription of genes, both complete and partially assembled Pol III complexes perform extra-transcriptional functions such as influencing nearby Pol II transcription, displacement of nearby nucleosomes, as well as chromatin boundary activities. By analyzing transcriptome data from high throughput RNA sequencing, we observed numerous alterations in intergenic transcription in close proximity to tDNAs and other Pol III complex binding sites after TFIIIC binding was globally compromised. Reduction of TFIIIC binding activity was achieved by using a yeast strain containing a mutation in the Reb1p binding site within the TFC6 promoter, which drastically reduces the level of the TFIIIC component Tfc6p. Analysis of loci adjacent to Pol III complex binding sites reveal both 5’- and 3’-extended transcripts, readthrough transcripts, and increased intergenic cryptic transcription. Many of the effects of 5’-UTR extension and de-repression appear to be due to the release of bidirectional activity of neighboring promoters. Translation of affected mRNAs is greatly altered because of the usage of upstream transcriptional start site (TSSs) at both TFC6 and TRM12 loci. The results presented here add another type of boundary activity to the known list of extra-transcriptional functions – the blocking activity of transcription from bidirectional promoters. Also, such activities might explain a function of the conserved ETC sites in yeast Saccharomyces cerevisiae. Analysis of the TFC6 locus suggests regulatory effects of assembled Pol III complexes at ETC6 site. Also, Reb1p is confirmed to be the transcriptional factor that binds and activates the TFC6 promoter and it binds several base pairs upstream of the ETC6 site. Analysis of these two divergently transcribed genes (ESC2 and TFC6) reveals that both have 5’-UTR extensions after Reb1p is depleted or the Reb1 consensus binding site is mutated, which is consistent with results from our transcriptome data. Similarly, altered TSS results in a significant decrease of the translational level of both TFC6 and ESC2. By assessing nucleosome content within the intergenic region ESC2-TFC6, we find that nucleosome positioning is slightly altered, which could explain the observed 5’-extended transcripts of both genes. Taken together, both of these studies demonstrate significant effects of assembled Pol III complexes and Reb1p binding on the transcription of neighboring Pol II promoters and on the translation of their mRNA products.

Subjects/Keywords: TFC6; Reb1; tRNA; TFIIIC complexes; Pol III complexes; Pol II

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Wang, Q. (2015). Regulatory Impacts of Assembled Pol III Complexes on Neighboring Pol II Transcribed Genes in Saccharomyces cerevisiae. (Doctoral Dissertation). Louisiana State University. Retrieved from etd-10302015-120951 ; https://digitalcommons.lsu.edu/gradschool_dissertations/2826

Chicago Manual of Style (16th Edition):

Wang, Qing. “Regulatory Impacts of Assembled Pol III Complexes on Neighboring Pol II Transcribed Genes in Saccharomyces cerevisiae.” 2015. Doctoral Dissertation, Louisiana State University. Accessed June 26, 2019. etd-10302015-120951 ; https://digitalcommons.lsu.edu/gradschool_dissertations/2826.

MLA Handbook (7th Edition):

Wang, Qing. “Regulatory Impacts of Assembled Pol III Complexes on Neighboring Pol II Transcribed Genes in Saccharomyces cerevisiae.” 2015. Web. 26 Jun 2019.

Vancouver:

Wang Q. Regulatory Impacts of Assembled Pol III Complexes on Neighboring Pol II Transcribed Genes in Saccharomyces cerevisiae. [Internet] [Doctoral dissertation]. Louisiana State University; 2015. [cited 2019 Jun 26]. Available from: etd-10302015-120951 ; https://digitalcommons.lsu.edu/gradschool_dissertations/2826.

Council of Science Editors:

Wang Q. Regulatory Impacts of Assembled Pol III Complexes on Neighboring Pol II Transcribed Genes in Saccharomyces cerevisiae. [Doctoral Dissertation]. Louisiana State University; 2015. Available from: etd-10302015-120951 ; https://digitalcommons.lsu.edu/gradschool_dissertations/2826


University of New Orleans

2. Ramsay, Milele. Effects of a putative Reb1 protein binding site on IME4 sense and antisense transcription and sporulation in Saccharomyces cerevisiae.

Degree: MS, Biological Sciences, 2009, University of New Orleans

Genome transcription is much more widespread than has been traditionally thought because our view of a "gene" or "transcription unit" has changed dramatically over the past 4 to 5 years with the identification of many different non-coding ribonucleic acids. In the yeast, Saccharomyces cerevisiae, meiosis and sporulation are an important part of the life cycle and IME4 gene expression is required for these processes. IME4 sense transcript levels of expression are influenced by the level of its complementary non-coding antisense strand by mechanisms that are currently unknown. The a1-alpha2 heterodimer binding in the downstream 3' region of IME4 is one component required for repression of IME4 antisense transcription. However, this thesis shows that the general regulatory protein Reb1 is also required in this system. Reb1 involvement is most likely to create a nucleosome-free zone in the promoter region of the IME4 antisense strand therefore contributing to transcription. Advisors/Committee Members: Clancy, Mary J., Liu, Zhengchang, Schluchter, Wendy.

Subjects/Keywords: Reb1 site mutant; IME4; sporulation; antisense transcription; regulation of ncRNA; a1-α2 repression; RNA strand-specific qPCR analysis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Ramsay, M. (2009). Effects of a putative Reb1 protein binding site on IME4 sense and antisense transcription and sporulation in Saccharomyces cerevisiae. (Thesis). University of New Orleans. Retrieved from https://scholarworks.uno.edu/td/1012

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Ramsay, Milele. “Effects of a putative Reb1 protein binding site on IME4 sense and antisense transcription and sporulation in Saccharomyces cerevisiae.” 2009. Thesis, University of New Orleans. Accessed June 26, 2019. https://scholarworks.uno.edu/td/1012.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Ramsay, Milele. “Effects of a putative Reb1 protein binding site on IME4 sense and antisense transcription and sporulation in Saccharomyces cerevisiae.” 2009. Web. 26 Jun 2019.

Vancouver:

Ramsay M. Effects of a putative Reb1 protein binding site on IME4 sense and antisense transcription and sporulation in Saccharomyces cerevisiae. [Internet] [Thesis]. University of New Orleans; 2009. [cited 2019 Jun 26]. Available from: https://scholarworks.uno.edu/td/1012.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ramsay M. Effects of a putative Reb1 protein binding site on IME4 sense and antisense transcription and sporulation in Saccharomyces cerevisiae. [Thesis]. University of New Orleans; 2009. Available from: https://scholarworks.uno.edu/td/1012

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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