Advanced search options

Advanced Search Options 🞨

Browse by author name (“Author name starts with…”).

Find ETDs with:

in
/  
in
/  
in
/  
in

Written in Published in Earliest date Latest date

Sorted by

Results per page:

Sorted by: relevance · author · university · dateNew search

You searched for subject:(Reagent Release). Showing records 1 – 2 of 2 total matches.

Search Limiters

Last 2 Years | English Only

No search limiters apply to these results.

▼ Search Limiters

1. Jahanshahi-Anbuhi, Sana. Development of Ready-to-Use Biosensors for Diagnostics and Biosensing.

Degree: DEng, 2014, McMaster University

Ideally, every person in the world should have access to a safe and clean water supply; if not all sources of water are clean and safe, at the very least, an effective method to detect water contamination should be readily available. An effective detection method should not only be sensitive, rapid, robust, and affordable, but, ideally, it should also be equipment-free and easy to transport and deliver to the end-users. The main goal of this project is to develop a variety of bits and pieces of bioassay systems, with a particular focus on paper-based bioactive devices in order to provide portable and ready-to-use biosensors which can be useable by anyone anywhere around the world without requiring formal training. According to the World Health Organization (WHO), 76,000 people each year die in India alone because of pesticide poisoning. Long term exposure to organophosphate pesticides is known to have adverse effects on neurological function and can lead to Alzheimer's Disease, Attention Deficit Hyperactivity Disorder (ADHD), and reduced Intelligence Quotient (IQ). The likelihood of long term exposure to pesticides is heightened in developing countries, so a reliable and inexpensive pesticide sensor is a much-needed device in the developing world. To address this need, this project reports on the development of a fully-automated bioactive paper-based sensor for the detection of organophosphate pesticides. In the proposed biosensor, two innovations were implemented to achieve a full-automated format for the pesticide sensor: (I) First is a PUMP ON A PAPER (Jahanshahi-Anbuhi et al., LOC, 2012) that increases the flow rate of fluids within paper-based microfluidic analytical devices and sequentially brings two separate liquid streams to the enzyme test zone on the paper sensor, and (II) the second innovation is a PIPETTE ON A PAPER (Jahanshahi-Anbuhi et al., LOC, 2014) that involved the creation of a pullulan (a natural non-ionic polysaccharide) temporary bridge-system to transfer a known amount of solution to the sensing zone that, gives the enzyme zone a chance to dry and accept the substrate solution from the slow channel after a fixed period of time. This proposed format results in a simplified assay that detects the presence of pesticides automatically without any further manipulation from the user. However, the shelf life of this assay kit is challenging due to instability of both enzyme (AChE) and substrate (IDA) at room temperature. AChE loses its enzymatic activity when stored at room temperature and IDA becomes oxidized quickly. This problem is not unique to these two bio reagents, however; almost all bioassays which use bio-reagents (such as enzymes and small-molecular substrates) are unstable to varying degrees and require special shipping and storage. The instability of these molecules can arise from either thermal denaturation or chemical modification, such as oxidation or hydrolysis. Because of these issues, they often have to be shipped on dry ice with special packaging, which is costly. The cost… Advisors/Committee Members: Pelton, Robert, Filipe, Carlos D.M., Chemical Engineering.

Subjects/Keywords: Biosensor; Microfluidic; Bio-active Paper; Paper Based Microfluidic Devices; Pullulan; Pill Based Assay; Reagent Release; Enzyme Stabilization; Organophosphate Pesticide; E. coli; Luciferase; Luminescent; Lab on Pills; Pullulan Tablet; Pullulan Capsule

Record DetailsSimilar RecordsGoogle PlusoneFacebookTwitterCiteULikeMendeleyreddit

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Jahanshahi-Anbuhi, S. (2014). Development of Ready-to-Use Biosensors for Diagnostics and Biosensing. (Doctoral Dissertation). McMaster University. Retrieved from http://hdl.handle.net/11375/16501

Chicago Manual of Style (16th Edition):

Jahanshahi-Anbuhi, Sana. “Development of Ready-to-Use Biosensors for Diagnostics and Biosensing.” 2014. Doctoral Dissertation, McMaster University. Accessed April 03, 2020. http://hdl.handle.net/11375/16501.

MLA Handbook (7th Edition):

Jahanshahi-Anbuhi, Sana. “Development of Ready-to-Use Biosensors for Diagnostics and Biosensing.” 2014. Web. 03 Apr 2020.

Vancouver:

Jahanshahi-Anbuhi S. Development of Ready-to-Use Biosensors for Diagnostics and Biosensing. [Internet] [Doctoral dissertation]. McMaster University; 2014. [cited 2020 Apr 03]. Available from: http://hdl.handle.net/11375/16501.

Council of Science Editors:

Jahanshahi-Anbuhi S. Development of Ready-to-Use Biosensors for Diagnostics and Biosensing. [Doctoral Dissertation]. McMaster University; 2014. Available from: http://hdl.handle.net/11375/16501

2. Miller, Samuel A. Treating Metals in Acid Mine Drainage Using Slow-Release Hydrogen Peroxide.

Degree: MS, Geological Sciences (Arts and Sciences), 2015, Ohio University

Metal concentrations from acid mine drainage (AMD) pose a significant threat to aquatic systems worldwide as a result of past and current mining operations. This study tested the efficacy of using slow-release hydrogen peroxide (SR-HP) to oxidize and remove ferrous iron (Fe2+) from AMD. Fenton’s reagent forms from a mixture of hydrogen peroxide (H2O2) and Fe2+ available from AMD, creating an advanced oxidation process. Twenty-eight SR-HP forms were developed by dispersing sodium percarbonate (Na2CO3 1.5H2O2) salts in a polymeric matrix. The SR-HP forms released H2O2 in flowing water at a peak release rate of 0.05 – 52.1 mg min-1 during the initial hour and continued to release H2O2 at a lower, stable release rate (0.02 – 1.5 mg min-1) from a period of days to weeks depending on salt : binding agent mixing ratios. Oxidant : resin mixing ratios in addition to surface area were primary factors impacting the release profiles from the laboratory column leaching tests. Proof-of-concept iron removal tests indicate that SR-HP forms can efficiently remove Fe2+ from AMD within one minute. Ideal [Fe+2]/[H2O2] ratios for >80% Fe2+ removal clustered around 2, with decreasing Fe2+ removal as the ratio increases. A small-scale field test demonstrated the efficacy of SR-HP at oxidizing Fe2+. Ferrous iron concentrations were reduced by 80% within the first hour of treatment. These results suggest feasibility of using SR-HP to treat oxidizable metals in AMD water. Further development of SR-HP forms with higher release rates, longer durations, stronger binder, and improved reproducibility could be possible. Advisors/Committee Members: Lee, Eung Seok (Advisor).

Subjects/Keywords: Environmental Science; Environmental Geology; Geology; Hydrology; Acid Mine Drainage; Fentons Reagent; Slow-Release

…3.1.1 Baseline Chemical Sampling €¦ €¦ €¦ €¦ €¦ €¦ €¦ €¦ €¦38 8 3.2 Characterizing Slow Release… …Systems €¦ €¦ €¦ €¦ €¦ €¦ €¦. €¦..40 3.2.1 Slow Release Form Dimensions €¦ €¦ €¦ €¦ €¦ €¦ €¦ €¦...40 3.2.2… …Column Release Tests €¦ €¦ €¦ €¦ €¦ €¦ €¦ €¦ €¦ €¦...41 3.2.3 Oxidant Release Efficiency / Recovery Rate… …sampled on 6/10/2014…. €¦β€¦.….39 Table 3-3. Properties of slow-release forms made with sodium… …Slow release diffusion modeled in cross sectional view. (Lee and Schwartz, 2007a)… 

Record DetailsSimilar RecordsGoogle PlusoneFacebookTwitterCiteULikeMendeleyreddit

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Miller, S. A. (2015). Treating Metals in Acid Mine Drainage Using Slow-Release Hydrogen Peroxide. (Masters Thesis). Ohio University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1430918808

Chicago Manual of Style (16th Edition):

Miller, Samuel A. “Treating Metals in Acid Mine Drainage Using Slow-Release Hydrogen Peroxide.” 2015. Masters Thesis, Ohio University. Accessed April 03, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1430918808.

MLA Handbook (7th Edition):

Miller, Samuel A. “Treating Metals in Acid Mine Drainage Using Slow-Release Hydrogen Peroxide.” 2015. Web. 03 Apr 2020.

Vancouver:

Miller SA. Treating Metals in Acid Mine Drainage Using Slow-Release Hydrogen Peroxide. [Internet] [Masters thesis]. Ohio University; 2015. [cited 2020 Apr 03]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1430918808.

Council of Science Editors:

Miller SA. Treating Metals in Acid Mine Drainage Using Slow-Release Hydrogen Peroxide. [Masters Thesis]. Ohio University; 2015. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1430918808

.