Advanced search options

Advanced Search Options 🞨

Browse by author name (“Author name starts with…”).

Find ETDs with:

in
/  
in
/  
in
/  
in

Written in Published in Earliest date Latest date

Sorted by

Results per page:

Language: English

You searched for subject:(QD IgE). One record found.

Search Limiters

Last 2 Years | English Only

No search limiters apply to these results.

▼ Search Limiters


University of New Mexico

1. Malik, Michael David. HYPERSPECTRAL LINE-SCANNING MICROSCOPY FOR HIGH-SPEED MULTICOLOR QUANTUM DOT TRACKING.

Degree: Physics & Astronomy, 2013, University of New Mexico

One of the challenges in studying protein interactions in live cells lies in the capacity to obtain both spatial and temporal information that is sufficient to extend existing knowledge of the dynamics and interactions, especially when tracking proteins at high density. Here we introduce a high-speed laser line-scanning hyperspectral microscope that is designed to track quantum dot labeled proteins at 27 frames/sec over an area of 28 um2 using 128 spectral channels spanning the range from 500 to 750 nm. This instrument simultaneously excites 8 species of quantum dots and employs a custom prism spectrometer and high speed EMCCD to obtain spectral information that is then used to distinguish and track individual probes at high density. These emitters are localized to within 10s of nm in each frame and reconstructed trajectories yield information of the protein dynamics and interactions. This manuscript describes the design, implementation, characterization, and application of a high-speed laser line-scanning hyperspectral microscope (HSM). The intended primary application is that of investigating the dynamics of transmembrane antibody receptors using quantum dot labeled immunoglobulin E (QD-IgE). Several additional examples demonstrate other advantages and applications of this method, including 3D hyperspectral imaging of live cells and hyperspectral superresolution imaging. Advisors/Committee Members: Lidke, Keith A., Lidke, Diane S., Thomas, James L., Prasad, Sudhakar.

Subjects/Keywords: confocal; hyperspectral; line-scanning; QD-IgE; quantum dot; SPT

Record DetailsSimilar RecordsGoogle PlusoneFacebookTwitterCiteULikeMendeleyreddit

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Malik, M. D. (2013). HYPERSPECTRAL LINE-SCANNING MICROSCOPY FOR HIGH-SPEED MULTICOLOR QUANTUM DOT TRACKING. (Doctoral Dissertation). University of New Mexico. Retrieved from http://hdl.handle.net/1928/23133

Chicago Manual of Style (16th Edition):

Malik, Michael David. “HYPERSPECTRAL LINE-SCANNING MICROSCOPY FOR HIGH-SPEED MULTICOLOR QUANTUM DOT TRACKING.” 2013. Doctoral Dissertation, University of New Mexico. Accessed June 04, 2020. http://hdl.handle.net/1928/23133.

MLA Handbook (7th Edition):

Malik, Michael David. “HYPERSPECTRAL LINE-SCANNING MICROSCOPY FOR HIGH-SPEED MULTICOLOR QUANTUM DOT TRACKING.” 2013. Web. 04 Jun 2020.

Vancouver:

Malik MD. HYPERSPECTRAL LINE-SCANNING MICROSCOPY FOR HIGH-SPEED MULTICOLOR QUANTUM DOT TRACKING. [Internet] [Doctoral dissertation]. University of New Mexico; 2013. [cited 2020 Jun 04]. Available from: http://hdl.handle.net/1928/23133.

Council of Science Editors:

Malik MD. HYPERSPECTRAL LINE-SCANNING MICROSCOPY FOR HIGH-SPEED MULTICOLOR QUANTUM DOT TRACKING. [Doctoral Dissertation]. University of New Mexico; 2013. Available from: http://hdl.handle.net/1928/23133

.