subject:(Pyruvate dehydrogenase complex Phosphoenolpyruvate carboxylase)

University of Georgia

1. Tomar, Atin. Production of pyruvate by Escherichia coli using metabolic engineering.

Degree: 2014, University of Georgia

► Pyruvate is a three-carbon (triose) ketoacid central to metabolism that could potentially be produced by fermentation. Previous studies observed pyruvate accumulation in strains having mutations… (more)

Subjects/Keywords: Pyruvate; Escherichia coli; Acetate mutants; Pyruvate Dehydrogenase complex; Phosphoenolpyruvate carboxylase; Pyruvate oxidase; NADH oxidase

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Tomar, A. (2014). Production of pyruvate by Escherichia coli using metabolic engineering. (Thesis). University of Georgia. Retrieved from http://hdl.handle.net/10724/29445

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Tomar, Atin. “Production of pyruvate by Escherichia coli using metabolic engineering.” 2014. Thesis, University of Georgia. Accessed April 13, 2021. http://hdl.handle.net/10724/29445.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Tomar, Atin. “Production of pyruvate by Escherichia coli using metabolic engineering.” 2014. Web. 13 Apr 2021.

Vancouver:

Tomar A. Production of pyruvate by Escherichia coli using metabolic engineering. [Internet] [Thesis]. University of Georgia; 2014. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/10724/29445.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Tomar A. Production of pyruvate by Escherichia coli using metabolic engineering. [Thesis]. University of Georgia; 2014. Available from: http://hdl.handle.net/10724/29445

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Georgia

2. Yang, Fei. Production of pyruvate by Escherichia coli using metabolic engineering.

Degree: 2014, University of Georgia

URL: http://hdl.handle.net/10724/22034

► Pyruvate is a three-carbon ketoacid produced at the end of glycolysis. This research involves the metabolic engineering of the bacterium Escherichia coli in order to… (more)

Subjects/Keywords: Pyruvate; Escherichia coli; Pyruvate dehydrogenase complex, Phosphoenolpyruvate carboxylase; Pyruvate oxidase; NADH oxidase; 2,4-dinitrophenol, Plackett-Burman design

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Yang, F. (2014). Production of pyruvate by Escherichia coli using metabolic engineering. (Thesis). University of Georgia. Retrieved from http://hdl.handle.net/10724/22034

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Yang, Fei. “Production of pyruvate by Escherichia coli using metabolic engineering.” 2014. Thesis, University of Georgia. Accessed April 13, 2021. http://hdl.handle.net/10724/22034.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Yang, Fei. “Production of pyruvate by Escherichia coli using metabolic engineering.” 2014. Web. 13 Apr 2021.

Vancouver:

Yang F. Production of pyruvate by Escherichia coli using metabolic engineering. [Internet] [Thesis]. University of Georgia; 2014. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/10724/22034.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Yang F. Production of pyruvate by Escherichia coli using metabolic engineering. [Thesis]. University of Georgia; 2014. Available from: http://hdl.handle.net/10724/22034

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Virginia Tech

3. Kraszewski, Jessica. Enzymology and Physiology of a New Type of Phosphoenolpyruvate Carboxylase and the Development of a Pyruvate Carboxylase Expression System.

Degree: MS, Biochemistry, 2007, Virginia Tech

URL: http://hdl.handle.net/10919/40966

► Our laboratory is interested in studying the junction of glycolysis and the tricarboxylic acid (TCA) cycle, specifically the enzymes phosphoenolpyruvate carboxykinase, pyruvate carboxylase and phosphoenolpyruvate… (more)

▼ Our laboratory is interested in studying the junction of glycolysis and the tricarboxylic acid (TCA) cycle, specifically the enzymes phosphoenolpyruvate carboxykinase, pyruvate carboxylase and phosphoenolpyruvate carboxylase. All produce oxaloacetate (OAA) for the cell. OAA production is critical for cell carbon synthesis in the methanogenic archaea. Therefore OAA-generating enzymes are essential for the survival of methanogens. In part of this study we investigated archaeal-type phosphoenolpyruvate carboxylase (PpcA), a new type of phosphoenolpyruvate carboxylase, which is widespread in the archaea and is found in three bacterial species. The form of phosphoenolpyruvate carboxylase (Ppc) that is prevalent in bacteria and plants is not found in the archaea. Due to complications expressing PpcA in the soluble form and difficulty purifying this enzyme from methanogens, an in-depth investigation of this enzymeâ s biochemical properties has yet to occur. In this study we demonstrate the successful expression of a PpcA homolog in the soluble fraction of Escherichia coli. We purified the recombinant protein to homogeneity. This development provides the means to study the enzymeâ s biochemical properties and manipulate the primary sequence in order to identify residues critical to the enzymeâ s function. We also show that this PpcA homolog does have the postulated activity and investigate its biochemical properties. The data show that PpcA has unique properties in regard to the enzymeâ s substrate and its regulation by metabolites. Our data also reveal that PpcA is a membrane associated protein, unlike Ppc, which is a soluble protein. We also show that pyruvate carboxylase (Pyc) can be expressed recombinantly in Pseudomonas aeruginosa at levels sufficient for structure-function studies. This is a major step forward in the expression in Pyc because it cannot be expressed at high levels in Escherichia coli. These are important developments in studying the enzymes that connect glycolysis and the TCA cycle. Advisors/Committee Members: Mukhopadhyay, Biswarup (committeechair), Dean, Dennis R. (committee member), Kennelly, Peter J. (committee member), White, Robert H. (committee member), Stevens, Ann M. (committee member).

Subjects/Keywords: methanogens; pyruvate carboxylase; phosphoenolpyruvate carboxylase; enzymology

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kraszewski, J. (2007). Enzymology and Physiology of a New Type of Phosphoenolpyruvate Carboxylase and the Development of a Pyruvate Carboxylase Expression System. (Masters Thesis). Virginia Tech. Retrieved from http://hdl.handle.net/10919/40966

Chicago Manual of Style (16^th Edition):

Kraszewski, Jessica. “Enzymology and Physiology of a New Type of Phosphoenolpyruvate Carboxylase and the Development of a Pyruvate Carboxylase Expression System.” 2007. Masters Thesis, Virginia Tech. Accessed April 13, 2021. http://hdl.handle.net/10919/40966.

MLA Handbook (7^th Edition):

Kraszewski, Jessica. “Enzymology and Physiology of a New Type of Phosphoenolpyruvate Carboxylase and the Development of a Pyruvate Carboxylase Expression System.” 2007. Web. 13 Apr 2021.

Vancouver:

Kraszewski J. Enzymology and Physiology of a New Type of Phosphoenolpyruvate Carboxylase and the Development of a Pyruvate Carboxylase Expression System. [Internet] [Masters thesis]. Virginia Tech; 2007. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/10919/40966.

Council of Science Editors:

Kraszewski J. Enzymology and Physiology of a New Type of Phosphoenolpyruvate Carboxylase and the Development of a Pyruvate Carboxylase Expression System. [Masters Thesis]. Virginia Tech; 2007. Available from: http://hdl.handle.net/10919/40966

University of Texas Southwestern Medical Center

4. Rajagopalan, Kartik N. The Role of Pyruvate Dehydrogenase in Cell Growth.

Degree: 2014, University of Texas Southwestern Medical Center

URL: http://hdl.handle.net/2152.5/5286

► Otto Warburg's observation that tumor cells have increased rates of glucose uptake and lactate secretion in comparison to normal cells spawned his notion that tumors… (more)

▼ Otto Warburg's observation that tumor cells have increased rates of glucose uptake and lactate secretion in comparison to normal cells spawned his notion that tumors have dysfunctional mitochondria. However, in addition to metabolizing glucose to lactate, tumors in vivo exhibit mitochondrial glucose oxidation, indicating activity of pyruvate dehydrognase (PDH), which gates entry of glucose derived carbon into the tricarboxylic acid (TCA) cycle. To test whether cells require glucose oxidation for proliferation, the work in this thesis establishes a model wherein PDH activity is suppressed using RNA interference. Small hairpin RNAs against the transcript encoding the PDHE1α protein were cloned into a retroviral vector which allowed doxycycline-inducible control of expression. Metabolism of cancer cells was studied in vitro using a combination of metabolomics and metabolic flux analysis. Growth in monolayer culture was performed in medium containing lipid-replete serum as well as serum in which lipids had been depleted. As expected, suppression of PDH activity reduced flow of carbon from glucose to the TCA cycle and to de novo fatty acid synthesis. Surprisingly, H460 lung cancer cells could tolerate a 60% reduction of PDH flux without any significant effect on proliferation rate, as long as lipids were present in the medium. Further examination of the effects of PDH silencing on the overall network of central carbon metabolism revealed enhanced channeling of carbon from glutamine to fatty acids and an increase in scavenging free fatty acids. Lipid depletion caused a reduction in growth rate of PDH deficient cells, and this defect was completely rescued by supplying free fatty acids to the medium. Together the data indicate that proliferating cells exhibit PDH activity that allows transfer of glucose carbon to citrate and the TCA cycle as well as ultimately into fatty acids. Importantly, suppression of PDH activity limits growth in conditions in which cancer cells do not have access to extracellular lipids. This work illustrates that compensatory pathways that sustain cell proliferation are activated during suppression of mitochondrial oxidation of glucose. Advisors/Committee Members: Amatruda, James F., DeBerardinis, Ralph J., Brown, Michael S., Lum, Lawrence.

Subjects/Keywords: Cell Proliferation; Fatty Acids; Glucose; Protein Kinases; Pyruvate Dehydrogenase Complex

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Rajagopalan, K. N. (2014). The Role of Pyruvate Dehydrogenase in Cell Growth. (Thesis). University of Texas Southwestern Medical Center. Retrieved from http://hdl.handle.net/2152.5/5286

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Rajagopalan, Kartik N. “The Role of Pyruvate Dehydrogenase in Cell Growth.” 2014. Thesis, University of Texas Southwestern Medical Center. Accessed April 13, 2021. http://hdl.handle.net/2152.5/5286.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Rajagopalan, Kartik N. “The Role of Pyruvate Dehydrogenase in Cell Growth.” 2014. Web. 13 Apr 2021.

Vancouver:

Rajagopalan KN. The Role of Pyruvate Dehydrogenase in Cell Growth. [Internet] [Thesis]. University of Texas Southwestern Medical Center; 2014. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/2152.5/5286.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Rajagopalan KN. The Role of Pyruvate Dehydrogenase in Cell Growth. [Thesis]. University of Texas Southwestern Medical Center; 2014. Available from: http://hdl.handle.net/2152.5/5286

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Glasgow

5. McGow, Donna. Cloning and characterisation of the plant pyruvate dehydrogenase complex components.

Degree: PhD, 2002, University of Glasgow

URL: http://theses.gla.ac.uk/72760/ ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.248232

► Multienzyme complexes are non-covalent assemblies composed of one or more copies of their constituent enzymes that act cooperatively to catalyse a linked series of reactions.… (more)

▼ Multienzyme complexes are non-covalent assemblies composed of one or more copies of their constituent enzymes that act cooperatively to catalyse a linked series of reactions. These multienzymes complexes are of great interest in the study of protein-protein interactions, substrate channelling and the catalytic and regulatory advantages conferred by their organisation into multimeric complexes. One such complex is pyruvate dehydrogenase complex (PDC), the principal member of the 2-oxo acid dehydrogenase complex family. PDC is located in the mitochondrial matrix in eukaryotes, where it catalyses the irreversible oxidative decarboxylation of pyruvate committing its acetyl-CoA product to enter into the citric acid cycle or ketone body formation. Plants are unique in having two distinct and spatially separate complexes; a mitochondrial complex similar to the mammalian and a chloroplastic complex with a unique anabolic function in providing acetyl-CoA and NADH for de novo fatty acid synthesis. Although much is known about the organisation and stoichiometry of the PDC from mammalian, yeast and prokaryotic sources little is known of the composition, subunit organisation and stoichiometry of the plant equivalent. Until recently, studies have been hindered by the low abundance of PDC in plant tissues. Nevertheless the plant PDC from both organelles are thought to be composed of the same three enzymes found in mammalian PDC designated E1, E2 and E3. In all PDCs studied to date E1 and E3 are arranged around an oligomeric E2 core. Two types of E2 core have been identified; the 24meric cube displaying octahedral (432) symmetry and the 60meric pentagonal dodecahedron displaying icosahedral (532) symmetry. In this thesis, various enzymes from plant mitochondrial and chloroplastic PDCs have been cloned from either recombinant plasmids containing the genes encoding PDC components or from cDNA produced from mRNA. These enzymes were cloned into different expression vectors that incorporate expression tags (His-tag or GST-fusion) to the N-terminal region of the recombinant enzymes and successfully expressed in E. coli. Subsequently these enzymes were purified to near homogeneity by Zn2+ affinity chromatography, as judged by Coomassie blue staining. High yields of enzymatically active recombinant enzymes were achieved using this purification method. The plant mitochondrial and chloroplastic E2 component was expressed as either the mature E2 enzyme or as a truncated form comprising the lipoyl domain(s) and adjacent subunit (E1 and/or E3) binding domain. Successful lipoylation of E2 indicates that these recombinant enzymes were capable of folding into their native 3D structures. A monoclonal antibody (mAb) specific for lipoylated human E3-BP and E2 was used to detect lipoylated plant E2s. However, this antibody only recognised the mitochondrial E2. The specificity of the antibody for mitochondrial E2s suggests that in addition to the presence of the lipoic acid cofactor the antibody also recognises regions of sequence specific to the…

Subjects/Keywords: 572; Mitochondrial pyruvate dehydrogenase complex

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

McGow, D. (2002). Cloning and characterisation of the plant pyruvate dehydrogenase complex components. (Doctoral Dissertation). University of Glasgow. Retrieved from http://theses.gla.ac.uk/72760/ ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.248232

Chicago Manual of Style (16^th Edition):

McGow, Donna. “Cloning and characterisation of the plant pyruvate dehydrogenase complex components.” 2002. Doctoral Dissertation, University of Glasgow. Accessed April 13, 2021. http://theses.gla.ac.uk/72760/ ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.248232.

MLA Handbook (7^th Edition):

McGow, Donna. “Cloning and characterisation of the plant pyruvate dehydrogenase complex components.” 2002. Web. 13 Apr 2021.

Vancouver:

McGow D. Cloning and characterisation of the plant pyruvate dehydrogenase complex components. [Internet] [Doctoral dissertation]. University of Glasgow; 2002. [cited 2021 Apr 13]. Available from: http://theses.gla.ac.uk/72760/ ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.248232.

Council of Science Editors:

McGow D. Cloning and characterisation of the plant pyruvate dehydrogenase complex components. [Doctoral Dissertation]. University of Glasgow; 2002. Available from: http://theses.gla.ac.uk/72760/ ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.248232

University of Adelaide

6. Bais, Renze. Pyruvate carboxylase : physical and kinetic studies.

Degree: 1974, University of Adelaide

URL: http://hdl.handle.net/2440/19966

Subjects/Keywords: Pyruvate carboxylase

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bais, R. (1974). Pyruvate carboxylase : physical and kinetic studies. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/19966

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Bais, Renze. “Pyruvate carboxylase : physical and kinetic studies.” 1974. Thesis, University of Adelaide. Accessed April 13, 2021. http://hdl.handle.net/2440/19966.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Bais, Renze. “Pyruvate carboxylase : physical and kinetic studies.” 1974. Web. 13 Apr 2021.

Vancouver:

Bais R. Pyruvate carboxylase : physical and kinetic studies. [Internet] [Thesis]. University of Adelaide; 1974. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/2440/19966.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Bais R. Pyruvate carboxylase : physical and kinetic studies. [Thesis]. University of Adelaide; 1974. Available from: http://hdl.handle.net/2440/19966

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Adelaide

7. Goss, Neil Howard. Pyruvate carboxylase: Physico-chemical aspects / a thesis submitted by Neil Howard Goss.

Degree: 1978, University of Adelaide

URL: http://hdl.handle.net/2440/20064

Subjects/Keywords: Pyruvate carboxylase

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Goss, N. H. (1978). Pyruvate carboxylase: Physico-chemical aspects / a thesis submitted by Neil Howard Goss. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/20064

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Goss, Neil Howard. “Pyruvate carboxylase: Physico-chemical aspects / a thesis submitted by Neil Howard Goss.” 1978. Thesis, University of Adelaide. Accessed April 13, 2021. http://hdl.handle.net/2440/20064.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Goss, Neil Howard. “Pyruvate carboxylase: Physico-chemical aspects / a thesis submitted by Neil Howard Goss.” 1978. Web. 13 Apr 2021.

Vancouver:

Goss NH. Pyruvate carboxylase: Physico-chemical aspects / a thesis submitted by Neil Howard Goss. [Internet] [Thesis]. University of Adelaide; 1978. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/2440/20064.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Goss NH. Pyruvate carboxylase: Physico-chemical aspects / a thesis submitted by Neil Howard Goss. [Thesis]. University of Adelaide; 1978. Available from: http://hdl.handle.net/2440/20064

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Adelaide

8. Phillips, Nelson Francis Boniface. Pyruvate carboxylase : studies on the reaction mechanism / by Nelson Francis Boniface Phillips.

Degree: 1981, University of Adelaide

URL: http://hdl.handle.net/2440/21134

Subjects/Keywords: Pyruvate carboxylase

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Phillips, N. F. B. (1981). Pyruvate carboxylase : studies on the reaction mechanism / by Nelson Francis Boniface Phillips. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/21134

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Phillips, Nelson Francis Boniface. “Pyruvate carboxylase : studies on the reaction mechanism / by Nelson Francis Boniface Phillips.” 1981. Thesis, University of Adelaide. Accessed April 13, 2021. http://hdl.handle.net/2440/21134.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Phillips, Nelson Francis Boniface. “Pyruvate carboxylase : studies on the reaction mechanism / by Nelson Francis Boniface Phillips.” 1981. Web. 13 Apr 2021.

Vancouver:

Phillips NFB. Pyruvate carboxylase : studies on the reaction mechanism / by Nelson Francis Boniface Phillips. [Internet] [Thesis]. University of Adelaide; 1981. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/2440/21134.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Phillips NFB. Pyruvate carboxylase : studies on the reaction mechanism / by Nelson Francis Boniface Phillips. [Thesis]. University of Adelaide; 1981. Available from: http://hdl.handle.net/2440/21134

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Adelaide

9. Easterbrook-Smith, Simon Barnett. The reaction mechanism of pyruvate carboxylase.

Degree: 1977, University of Adelaide

URL: http://hdl.handle.net/2440/37988

► 1. The 2 ', 3 ' -dialdehyde derivative of ATP (oATP) was prepared by periodate oxidation, and on the following criteria was considered to be… (more)

▼ 1. The 2 ', 3 ' -dialdehyde derivative of ATP (oATP) was prepared by periodate oxidation, and on the following criteria was considered to be an affinity label of the MgATP ²⁻ binding site of sheep liver pyruvate carboxylase. The magnesium complex of this inhibitor (Mg-oATP ² ⁻ ) was shown to be a linear competitive inhibitor with respect to MgATP ² ⁻ in both the acetyl CoA-dependent and -independent activities of the enzyme but was a non-competitive inhibitor with respect to bicarbonate and an un-competitive inhibitor with respect to pyruvate. Mg-oATP ² ⁻ was covalently bound to pyruvate carboxylase by reduction using sodium borohydride with concurrent irreversible inactivation of the enzyme. Although pyruvate, oxaloacetate, and bicarbonate were ineffective, both acetyl CoA and MgATP ² ⁻ protected the enzyme against this chemical modification. Mg ² ⁺ enhanced the extent of chemical modification. At 100% inactivation, 1.1 ± 0.1 moles of Mg-oATP ² ⁻ were bound to the enzyme per mole of biotin. The presence or absence of acetyl CoA had no affect on this stoichiometry. Chromatography of samples of an enzymic digest of Mg - o [¹ ⁴ C ] ATP ² ⁻ -labelled enzyme revealed one major band of radioactivity which co-chromatographed with authentic lys-oATP markers. 2. In order to further characterise the lysyl residue (s) in the biotin carboxylation site, the use of cyanate as a potential affinity label of the bicarbonate binding site was investigated. Cyanate was shown to chemically modify sheep liver pyruvate carboxylase, with concurrent inactivation of the enzyme. The rate of loss of acetyl CoA-independent catalytic activity was not pseudo first-order with respect to time. When the inactivation data were analysed assuming that modified enzyme retains some residual acetyl CoA-independent activity, it was found that only one kinetically significant residue per active site was modified, but cyanate appeared to form a reversible complex with the enzyme prior to modifying this residue. Acetyl CoA, pyruvate and oxaloacetate were found to have no affect on the rate of modification of the enzyme. Bicarbonate, MgADP, and MgATP ² ⁻ all afforded protection against inactivation of the enzyme. MgATP2 ⁻ afforded almost complete protection against loss of both the acetyl CoA-dependent and - independent catalytic activities. Analysis of the concentration dependence of protection by bicarbonate and MgATP ² ⁻ suggested that cyanate could not inactivate the enzyme - MgATP ² ⁻ and enzyme-bicarbonate complexes. The enzyme MgADP was found to be inactivated at about 30% of the rate of free enzyme. Modification of the enzyme with cyanate was found to inactivate the ATP/P [subscript i] isotopic exchange reaction, but to enhance the pyruvate/oxaloacetate isotopic exchange reaction. Inactivation of pyruvate carboxylase with [¹ ⁴ C ] cyanate was shown to be associated with modification of at least six amino acid residues per mole of biotin. Chromato-graphy of samples of an enzyme digest of [¹ ⁴ C ] cyanate-labelled enzyme revealed that… Advisors/Committee Members: Keech, Donald Bruce (advisor), Wallace, John Campbell (advisor), Dept. of Biochemistry (school).

Subjects/Keywords: pyruvate carboxylase

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Easterbrook-Smith, S. B. (1977). The reaction mechanism of pyruvate carboxylase. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/37988

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Easterbrook-Smith, Simon Barnett. “The reaction mechanism of pyruvate carboxylase.” 1977. Thesis, University of Adelaide. Accessed April 13, 2021. http://hdl.handle.net/2440/37988.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Easterbrook-Smith, Simon Barnett. “The reaction mechanism of pyruvate carboxylase.” 1977. Web. 13 Apr 2021.

Vancouver:

Easterbrook-Smith SB. The reaction mechanism of pyruvate carboxylase. [Internet] [Thesis]. University of Adelaide; 1977. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/2440/37988.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Easterbrook-Smith SB. The reaction mechanism of pyruvate carboxylase. [Thesis]. University of Adelaide; 1977. Available from: http://hdl.handle.net/2440/37988

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

10. Burkett, Daniel John. Synthesis and Evaluation of Small Molecule Inhibitors of Pyruvate Carboxylase.

Degree: 2020, Marquette University

URL: https://epublications.marquette.edu/theses_open/604

► Through structure-based drug design (SBDD), a series of small molecules have been synthesized and evaluated for inhibitory activity against the pyruvate carboxylase (PC) enzyme via… (more)

Subjects/Keywords: Hydroxycinnamic acids; Pyruvate carboxylase; Chemistry

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Burkett, D. J. (2020). Synthesis and Evaluation of Small Molecule Inhibitors of Pyruvate Carboxylase. (Thesis). Marquette University. Retrieved from https://epublications.marquette.edu/theses_open/604

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Burkett, Daniel John. “Synthesis and Evaluation of Small Molecule Inhibitors of Pyruvate Carboxylase.” 2020. Thesis, Marquette University. Accessed April 13, 2021. https://epublications.marquette.edu/theses_open/604.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Burkett, Daniel John. “Synthesis and Evaluation of Small Molecule Inhibitors of Pyruvate Carboxylase.” 2020. Web. 13 Apr 2021.

Vancouver:

Burkett DJ. Synthesis and Evaluation of Small Molecule Inhibitors of Pyruvate Carboxylase. [Internet] [Thesis]. Marquette University; 2020. [cited 2021 Apr 13]. Available from: https://epublications.marquette.edu/theses_open/604.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Burkett DJ. Synthesis and Evaluation of Small Molecule Inhibitors of Pyruvate Carboxylase. [Thesis]. Marquette University; 2020. Available from: https://epublications.marquette.edu/theses_open/604

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Louisville

11. Sellers, Katherine. Pyruvate carboxylase is upregulated in NSCLC to support proliferation and anabolism.

Degree: PhD, 2013, University of Louisville

URL: 10.18297/etd/1297 ; https://ir.library.louisville.edu/etd/1297

► Non-small cell lung cancer (NSCLC) is the number one cause of cancer-related death and, like all cancers, requires metabolic reprogramming to support the energetic… (more)

▼ Non-small cell lung cancer (NSCLC) is the number one cause of cancer-related death and, like all cancers, requires metabolic reprogramming to support the energetic and biosynthetic demands of the tumor cells to proliferate and survive the tumor microenvironment. This dissertation sought to uncover novel metabolic dysfunctions in NSCLC that may lead to new, targeted therapeutic strategies. To this end, a stable isotope-resolved metabolomics approach was used to study NSCLC in human patients in vivo, in cell culture, and in mouse xenograft tumors. This approach involves treating biological samples with tracers containing stable isotopes, such as 13C6-glucose, and monitoring the incorporation of the isotope into metabolites by mass spectrometry and nuclear magnetic resonance spectroscopy. In Chapter 2, NSCLC-associated metabolic dysfunctions were elucidated by infusing human NSCLC patients with 13C6-glucose prior to tumor resection. Metabolite concentrations and labeling patterns in tumor tissues were compared to surrounding benign lung tissue from the same patient. The major findings were that tumor tissues had enhanced flux through glycolysis, the pentose phosphate pathway, glycogen synthesis and utilization, pyruvate dehydrogenase, malic enzyme, nucleotide biosynthesis, glycine biosynthesis, and UDP-GlcNAc biosynthesis. Particularly interesting was the increased rate of glucose oxidation through the TCA cycle, accompanied by increased utilization of TCA cycle intermediates for biosynthesis. To support this increase in TCA cycle flux, the expression and activity of the anapleurotic enzyme pyruvate carboxylase (PC) was enhanced in human NSCLC tumors. In Chapter 3, PC expression was suppressed in NSCLC cell lines by shRNA. The phenotypic and metabolic consequences were monitored in vitro and in mouse xenografts. Silencing PC reduced NSCLC cell growth, tumorigenicity, and survival. It was demonstrated that PC serves several important functions in NSCLC, including supporting mitochondrial activity, macromolecule biosynthesis especially lipids, and maintaining the redox status of the cell. In Chapter 4, small molecules were employed to inhibit PC activity in vitro, including a known anticancer agent methylseleninic acid (MSA). This is the first report demonstrating that MSA inhibits PC activity in vitro and in cell culture. Several other molecules with anti-PC activity were elucidated by in silico screening. Like PC knockdown, these inhibitors reduced NSCLC cell growth, tumorigenicity, and macromolecule biosynthesis. Together, these data indicate that PC is upregulated in NSCLC and functions to support anabolic processes critical to NSCLC proliferation and survival. Advisors/Committee Members: Fan, Teresa.

Subjects/Keywords: Cancer; Metabolism; Pyruvate carboxylase

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sellers, K. (2013). Pyruvate carboxylase is upregulated in NSCLC to support proliferation and anabolism. (Doctoral Dissertation). University of Louisville. Retrieved from 10.18297/etd/1297 ; https://ir.library.louisville.edu/etd/1297

Chicago Manual of Style (16^th Edition):

Sellers, Katherine. “Pyruvate carboxylase is upregulated in NSCLC to support proliferation and anabolism.” 2013. Doctoral Dissertation, University of Louisville. Accessed April 13, 2021. 10.18297/etd/1297 ; https://ir.library.louisville.edu/etd/1297.

MLA Handbook (7^th Edition):

Sellers, Katherine. “Pyruvate carboxylase is upregulated in NSCLC to support proliferation and anabolism.” 2013. Web. 13 Apr 2021.

Vancouver:

Sellers K. Pyruvate carboxylase is upregulated in NSCLC to support proliferation and anabolism. [Internet] [Doctoral dissertation]. University of Louisville; 2013. [cited 2021 Apr 13]. Available from: 10.18297/etd/1297 ; https://ir.library.louisville.edu/etd/1297.

Council of Science Editors:

Sellers K. Pyruvate carboxylase is upregulated in NSCLC to support proliferation and anabolism. [Doctoral Dissertation]. University of Louisville; 2013. Available from: 10.18297/etd/1297 ; https://ir.library.louisville.edu/etd/1297

Queens University

12. O'Leary, Brendan Michael. In vivo regulatory phosphorylation of bacterial-type phosphoenolpyruvate carboxylase from developing castor oil seeds .

Degree: Biology, 2011, Queens University

URL: http://hdl.handle.net/1974/6705

► PEPC [PEP(phosphoenolpyruvate) carboxylase] is an essential and tightly controlled enzyme located at the core of plant C-metabolism. It fulfils a broad spectrum of non-photosynthetic functions,… (more)

▼ PEPC [PEP(phosphoenolpyruvate) carboxylase] is an essential and tightly controlled enzyme located at the core of plant C-metabolism. It fulfils a broad spectrum of non-photosynthetic functions, particularly the anaplerotic replenishment of tricarboxylic acid cycle intermediates consumed during biosynthesis and N-assimilation. In plants, a small multigene family encodes several closely related plant-type PEPC (PTPC) isozymes along with a distantly related bacterial-type PEPC (BTPC) isozyme. The PTPCs are well studied ~110-kDa subunits that typically exist as a homotetramer (Class-1 PEPC). By contrast, little is known about the larger ~118-kDa BTPC isozyme except that it occurs in developing castor (Ricinus communis) endosperm in tight association with PTPC subunits as a ~900-kDa hetero-octameric complex (Class-2 PEPC) that is greatly desensitized to metabolic effectors compared to Class-1 PEPC. This thesis elucidates the physiological purpose of the BTPC subunits by examining their structure/function relationship within Class-2 PEPC and identifying mechanisms of post-translational control. Recombinant expression and purification of the castor bean BTPC revealed unusual physical and kinetic properties including a remarkable insensitivity to metabolic effectors and a dependence upon PTPC subunits for structural stability. The first purification of a non-proteolyzed plant Class-2 PEPC complex was performed, and the kinetic analysis determined that the BTPC and PTPC subunits have complimentary catalytic properties. The BTPC subunits’ high Km(PEP) and desensitization to metabolic effectors may function as a metabolic overflow mechanism for sustaining flux from PEP to malate when PTPC subunits become feedback inhibited. An anti-PTPC co-immunopurification strategy was utilized to highly enrich non-proteolyzed BTPC from developing castor endosperm for downstream immunological and mass spectrometric analysis. BTPC was in vivo phosphorylated at multiple novel sites, identified by mass spectrometry as Thr4 or 5, Ser425 and Ser451. Phosphosite-specific antibodies towards Ser425 and Ser451 confirmed the existence of these sites in vivo and comparisons of Ser425 phosphorylation patterns established that the castor BTPC and PTPC phosphorytation sites are regulated independently. Phosphomimetic mutants of Ser425 caused BTPC inhibition by increasing its Km(PEP) and sensitivity to feedback inhibition. These results establish a novel mechanism of PEPC control whose implications within plant carbon metabolism are discussed.

Subjects/Keywords: oilseed metabolism ; regulatory phosphorylation ; phosphoenolpyruvate carboxylase

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

O'Leary, B. M. (2011). In vivo regulatory phosphorylation of bacterial-type phosphoenolpyruvate carboxylase from developing castor oil seeds . (Thesis). Queens University. Retrieved from http://hdl.handle.net/1974/6705

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

O'Leary, Brendan Michael. “In vivo regulatory phosphorylation of bacterial-type phosphoenolpyruvate carboxylase from developing castor oil seeds .” 2011. Thesis, Queens University. Accessed April 13, 2021. http://hdl.handle.net/1974/6705.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

O'Leary, Brendan Michael. “In vivo regulatory phosphorylation of bacterial-type phosphoenolpyruvate carboxylase from developing castor oil seeds .” 2011. Web. 13 Apr 2021.

Vancouver:

O'Leary BM. In vivo regulatory phosphorylation of bacterial-type phosphoenolpyruvate carboxylase from developing castor oil seeds . [Internet] [Thesis]. Queens University; 2011. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/1974/6705.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

O'Leary BM. In vivo regulatory phosphorylation of bacterial-type phosphoenolpyruvate carboxylase from developing castor oil seeds . [Thesis]. Queens University; 2011. Available from: http://hdl.handle.net/1974/6705

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Mississippi State University

13. Albader, Anoud Abdulmalik. Regulation of stomata opening in the crassulacean acid metabolism plant Kalanchoe laxiflora.

Degree: MS, Biochemistry, Molecular Biology, Entomology and Plant Pathology, 2017, Mississippi State University

URL: http://sun.library.msstate.edu/ETD-db/theses/available/etd-10242017-105759/ ;

► Stomata are small pores that are located on the surface of epidermal leaves, and they can regulate the uptake of CO2 and prevent water… (more)

Subjects/Keywords: malic acid; citric acid; malate dehydrogenase; phosphoenolpyruvate carboxylase; RUBISCO; Native gel electrophoresis;

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Albader, A. A. (2017). Regulation of stomata opening in the crassulacean acid metabolism plant Kalanchoe laxiflora. (Masters Thesis). Mississippi State University. Retrieved from http://sun.library.msstate.edu/ETD-db/theses/available/etd-10242017-105759/ ;

Chicago Manual of Style (16^th Edition):

Albader, Anoud Abdulmalik. “Regulation of stomata opening in the crassulacean acid metabolism plant Kalanchoe laxiflora.” 2017. Masters Thesis, Mississippi State University. Accessed April 13, 2021. http://sun.library.msstate.edu/ETD-db/theses/available/etd-10242017-105759/ ;.

MLA Handbook (7^th Edition):

Albader, Anoud Abdulmalik. “Regulation of stomata opening in the crassulacean acid metabolism plant Kalanchoe laxiflora.” 2017. Web. 13 Apr 2021.

Vancouver:

Albader AA. Regulation of stomata opening in the crassulacean acid metabolism plant Kalanchoe laxiflora. [Internet] [Masters thesis]. Mississippi State University; 2017. [cited 2021 Apr 13]. Available from: http://sun.library.msstate.edu/ETD-db/theses/available/etd-10242017-105759/ ;.

Council of Science Editors:

Albader AA. Regulation of stomata opening in the crassulacean acid metabolism plant Kalanchoe laxiflora. [Masters Thesis]. Mississippi State University; 2017. Available from: http://sun.library.msstate.edu/ETD-db/theses/available/etd-10242017-105759/ ;

14. Rahimi, Yasmeen. The role of pyruvate dehydrogenase kinase in glucose and ketone body metabolism.

Degree: 2014, IUPUI

URL: http://hdl.handle.net/1805/3798

►

Indiana University-Purdue University Indianapolis (IUPUI)

The expression of pyruvate dehydrogenase kinase (PDK) 2 and 4 are increased in the fasted state to inactivate the pyruvate… (more)

▼

Indiana University-Purdue University Indianapolis (IUPUI)

The expression of pyruvate dehydrogenase kinase (PDK) 2 and 4 are increased in the fasted state to inactivate the pyruvate dehydrogenase complex (PDC) by phosphorylation to conserve substrates for glucose production. To assess the importance of PDK2 and PDK4 in regulation of the PDC to maintain glucose homeostasis, PDK2 knockout (KO), PDK4 KO, and PDK2/PDK4 double knockout (DKO) mice were generated. PDK2 deficiency caused higher PDC activity and lower blood glucose levels in the fed state while PDK4 deficiency caused similar effects in the fasting state. DKO intensified these effects in both states. PDK2 deficiency had no effect on glucose tolerance, PDK4 deficiency produced a modest effect, but DKO caused a marked improvement, lowered insulin levels, and increased insulin sensitivity. However, the DKO mice were more sensitive than wild-type mice to long term fasting, succumbing to hypoglycemia, ketoacidosis, and hypothermia. Stable isotope flux analysis indicated that hypoglycemia was due to a reduced rate of gluconeogenesis. We hypothesized that hyperglycemia would be prevented in DKO mice fed a high saturated fat diet for 30 weeks. As expected, DKO mice fed a high fat diet had improved glucose tolerance, decreased adiposity, and were euglycemic due to reduction in the rate of gluconeogenesis. Like chow fed DKO mice, high fat fed DKO mice were unusually sensitive to fasting because of ketoacidosis and hypothermia. PDK deficiency resulted in greater PDC activity which limited the availability of pyruvate for oxaloacetate synthesis. Low oxaloacetate resulted in overproduction of ketone bodies by the liver and inhibition of ketone body and fatty acid oxidation by peripheral tissues, culminating in ketoacidosis and hypothermia. Furthermore, when fed a ketogenic diet consisting of low carbohydrate and high fat, DKO mice also exhibited hypothermia, ketoacidosis, and hypoglycemia. The findings establish that PDK2 is more important in the fed state, PDK4 is more important in the fasted state, survival during long term fasting depends upon regulation of the PDC by both PDK2 and PDK4, and that the PDKs are important for the regulation of glucose and ketone body metabolism.

Advisors/Committee Members: Harris, Robert A. (Robert Allison), 1939-, Considine, Robert V., Roach, Peter J., Wek, Ronald C..

Subjects/Keywords: pyruvate dehydrogenase complex, ketoacidosis, hypothermia, fatty acid, pyruvate carboxylase; Ketone body metabolism – Research; Pyruvate kinase – Research; Blood sugar – Regulation; Sugar in the body; Hypoglycemia; Ketoacidosis; Hypothermia; Gluconeogenesis – Research; Fatty acids – Synthesis; Pyruvate carboxylase; Ketogenic diet; Mice as laboratory animals – Research

…translocation ...........................1 2. Regulation of pyruvate dehydrogenase complex by… …responsible for regulation of pyruvate dehydrogenase complex 2.1. Regulation of pyruvate… …dehydrogenase complex The PDC is inactivated by phosphorylation by pyruvate dehyrogenase kinases (… …Regulation of pyruvate dehydrogenase complex by phosphorylation and allosteric effectors [46… …gluconeogenensis consists of eleven enzyme-catalyzed reactions, phosphoenolpyruvate carboxylase (…

10 more images…

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Rahimi, Y. (2014). The role of pyruvate dehydrogenase kinase in glucose and ketone body metabolism. (Thesis). IUPUI. Retrieved from http://hdl.handle.net/1805/3798

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Rahimi, Yasmeen. “The role of pyruvate dehydrogenase kinase in glucose and ketone body metabolism.” 2014. Thesis, IUPUI. Accessed April 13, 2021. http://hdl.handle.net/1805/3798.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Rahimi, Yasmeen. “The role of pyruvate dehydrogenase kinase in glucose and ketone body metabolism.” 2014. Web. 13 Apr 2021.

Vancouver:

Rahimi Y. The role of pyruvate dehydrogenase kinase in glucose and ketone body metabolism. [Internet] [Thesis]. IUPUI; 2014. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/1805/3798.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Rahimi Y. The role of pyruvate dehydrogenase kinase in glucose and ketone body metabolism. [Thesis]. IUPUI; 2014. Available from: http://hdl.handle.net/1805/3798

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

The Ohio State University

15. Ikeda, Bryan Hiroshi. Subunit structure and function of PDH complex from Escherichia coli.

Degree: PhD, Graduate School, 1977, The Ohio State University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=osu1487070226256534

Subjects/Keywords: Chemistry; Escherichia coli; Pyruvate dehydrogenase complex

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ikeda, B. H. (1977). Subunit structure and function of PDH complex from Escherichia coli. (Doctoral Dissertation). The Ohio State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=osu1487070226256534

Chicago Manual of Style (16^th Edition):

Ikeda, Bryan Hiroshi. “Subunit structure and function of PDH complex from Escherichia coli.” 1977. Doctoral Dissertation, The Ohio State University. Accessed April 13, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487070226256534.

MLA Handbook (7^th Edition):

Ikeda, Bryan Hiroshi. “Subunit structure and function of PDH complex from Escherichia coli.” 1977. Web. 13 Apr 2021.

Vancouver:

Ikeda BH. Subunit structure and function of PDH complex from Escherichia coli. [Internet] [Doctoral dissertation]. The Ohio State University; 1977. [cited 2021 Apr 13]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1487070226256534.

Council of Science Editors:

Ikeda BH. Subunit structure and function of PDH complex from Escherichia coli. [Doctoral Dissertation]. The Ohio State University; 1977. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1487070226256534

The Ohio State University

16. CaJacob, Claire Ann. E. Coli pyruvate dehydrogenase complex : studies on the mechanism of action and subunit composition of the complex.

Degree: PhD, Graduate School, 1984, The Ohio State University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=osu1487251434781173

Subjects/Keywords: Chemistry; Escherichia coli; PYRUVATE DEHYDROGENASE COMPLEX

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

CaJacob, C. A. (1984). E. Coli pyruvate dehydrogenase complex : studies on the mechanism of action and subunit composition of the complex. (Doctoral Dissertation). The Ohio State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=osu1487251434781173

Chicago Manual of Style (16^th Edition):

CaJacob, Claire Ann. “E. Coli pyruvate dehydrogenase complex : studies on the mechanism of action and subunit composition of the complex.” 1984. Doctoral Dissertation, The Ohio State University. Accessed April 13, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487251434781173.

MLA Handbook (7^th Edition):

CaJacob, Claire Ann. “E. Coli pyruvate dehydrogenase complex : studies on the mechanism of action and subunit composition of the complex.” 1984. Web. 13 Apr 2021.

Vancouver:

CaJacob CA. E. Coli pyruvate dehydrogenase complex : studies on the mechanism of action and subunit composition of the complex. [Internet] [Doctoral dissertation]. The Ohio State University; 1984. [cited 2021 Apr 13]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1487251434781173.

Council of Science Editors:

CaJacob CA. E. Coli pyruvate dehydrogenase complex : studies on the mechanism of action and subunit composition of the complex. [Doctoral Dissertation]. The Ohio State University; 1984. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1487251434781173

17. Naik, Sharon S. Regulation of the pyruvate dehydrogenase complex.

Degree: PhD, Biochemistry, 1995, Case Western Reserve University School of Graduate Studies

URL: http://rave.ohiolink.edu/etdc/view?acc_num=case1062509957

► The mammalian pyruvate dehydrogenase complex (PDC) is subjected to both short-term (product inhibition and covalent modification) and long term (increases in total activity and protein… (more)

▼ The mammalian pyruvate dehydrogenase complex (PDC) is subjected to both short-term (product inhibition and covalent modification) and long term (increases in total activity and protein mass) regulation mediated by dietary and hormonal treatments. Initial research efforts were focused on the isolation and characterization of the human E1β gene. The 2.3 kb E1β promoter region lacked a TATA box homology but contained initiator sequences (two) and Sp1 sites (three) frequently found in TATA-less promoters that may play a role in defining the start site of transcription. Transient expression of chloramphenicol acetyltransferase (CAT) activity of nested deletions of the E1β 5′-flanking region (-2316 to +32 base pairs) measured in human hepatoma (HepG2) cells, suggested the possible presence of repressor elements located between sequences -2316 to -930 base pairs. Deletion constructs containing sequences from -929 to +32 and -98 to +32 base pair showed approximately 7- and 20-fold increases in CAT over that containing -2316 to +32 base pairs of the E1β promoter region. The presence of a repressor element between -2316 to -929 base pairs was further confirmed by measuring CAT activity from a heterologous thymidine kinase promoter. Similar studies on the measurement of promoter activity of a 796 base pair E1α promoter fragment (-763 to +33 bp) generated by polymerase chain reaction (PCR) from normal human skin fibroblasts was measured in HepG2 cells. Several sequences showing consensus with known functional elements have been identified within the 5′-flanking region of the human E1α gene. They include the following: activator protein AP-1, cAMP-responsive element (CRE), two activator proteins (AP-2), CCAAT/enhancer binding protein (C/EBP) and an insulin-responsive sequence (IRS). It was observed that a construct containing the 796 base pair fragment (-763 to +33) resulted in very high CAT expression comparable to that derived from pSV2CAT (positive control). The smallest construct, containing only 102 base pairs of the promoter region resulted in a significant decrease in CAT activity, but was still capable of efficiently promoting CAT expression. The E1α promoter (-763 to +33) resembles facultative promoters in having appropriately positioned "TATA" and "CAAT" boxes with several upstream potential enhancer sequences which allows for efficient expression of CAT in HepG2 cells. Diet-induced long-term regulation of PDC appears to involve coordinated modulation of all the component proteins of the complex. The present study was carried out to evaluate the long-term effects of high-sucrose and high-fat diets on PDC activity in the liver. Rats fed a high-sucrose diet for two and three weeks resulted in a 2-fold increase in total activity compared to chow-fed rats. Changes in total PDC activity closely correlated with alterations in the amount of proteins quantified by immunoblotting, suggesting that increased enzyme content is the predominant mechanism underlying the adaptive response to high-sucrose feeding. There was no… Advisors/Committee Members: Patel, Mulchand (Advisor).

Subjects/Keywords: Biology, Molecular; Pyruvate dehydrogenase complex (PDC)

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Naik, S. S. (1995). Regulation of the pyruvate dehydrogenase complex. (Doctoral Dissertation). Case Western Reserve University School of Graduate Studies. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=case1062509957

Chicago Manual of Style (16^th Edition):

Naik, Sharon S. “Regulation of the pyruvate dehydrogenase complex.” 1995. Doctoral Dissertation, Case Western Reserve University School of Graduate Studies. Accessed April 13, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=case1062509957.

MLA Handbook (7^th Edition):

Naik, Sharon S. “Regulation of the pyruvate dehydrogenase complex.” 1995. Web. 13 Apr 2021.

Vancouver:

Naik SS. Regulation of the pyruvate dehydrogenase complex. [Internet] [Doctoral dissertation]. Case Western Reserve University School of Graduate Studies; 1995. [cited 2021 Apr 13]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=case1062509957.

Council of Science Editors:

Naik SS. Regulation of the pyruvate dehydrogenase complex. [Doctoral Dissertation]. Case Western Reserve University School of Graduate Studies; 1995. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=case1062509957

Stellenbosch University

18. Stevens, Gary. The regulation of Phosphoenolpyruvate (PEP) metabolism via Phosphoenolpyruvate Carboxylase (PEPC) in P-deficient roots and nodules of Virgilia divaricata.

Degree: PhD, Botany and Zoology, 2015, Stellenbosch University

URL: http://hdl.handle.net/10019.1/97853

► ENGLISH ABSTRACT: Plants exhibit a flexible array of morphological, physiological and biochemical adaptations during phosphorous limitation. Legumes are vulnerable to P deficiency, because it affects… (more)

▼ ENGLISH ABSTRACT: Plants exhibit a flexible array of morphological, physiological and biochemical adaptations during phosphorous limitation. Legumes are vulnerable to P deficiency, because it affects their ability to fix atmospheric nitrogen (N2). In particular, legumes from nutrient-poor ecosystems, such as the Fynbos in the Cape Floristic Region (CFR) evolved on P deficient soils and may therefore display unique adaptations to soil P stress. In general, very few studies on legumes have focussed on the belowground structures of nodules as a plant organ. Moreover, even less is known about the P stressed responses in nodules from legumes in nutrient-poor ecosystems. The aim of this research was to investigate the metabolic flexibility of organic acid and amino acid metabolism in the nodulated root system of the Fynbos legume Virgilia divaricata, during low P stress. Virgilia divaricata, which grows in the Cape Floristic Region, was used in this study to enhance our knowledge regarding the vital role that the cytosolic enzyme, phosphoenol pyruvate carboxylase (PEPC) plays in phosphoenol pyruvate (PEP) metabolism, in roots and nodules of this legume during phosphate stress. V. divaricata was grown under glasshouse conditions (20 - 25°C) in sterilized quartz sand for 2-3 months whilst being inoculated with the nitrogen fixation bacteria, Burkholderia phytofirmans, which was isolated from V. divaricata nodules grown in fynbos soil. Two phosphate treatments, 5 μM and 500 μM, were applied simulating low-phosphate and high phosphate conditions respectively using a modified Long Ashton Nutrient Solution to simulate a low nutrient ecosystem such as the Cape Floristic Region. Roots and nodules were then analysed for growth kinetics, nutrient acquisition and distribution, enzyme activity and genetic responses. It was shown that during phosphate deficiency, V. divaricata nodules experienced less Pi stress than roots, due to increased metabolic phosphate conservation reactions during organic acid synthesis via an increased PEPC activity. The increased PEPC activity resulted in an increase in downstream metabolic products such as organic acids, (malic acid and citric acid), and amino acids (glutamate, aspartate and asparagine). Although the biological nitrogen fixation (BNF) declined, the high efficiency of BNF may be underpinned by these altered phosphate conservation pathways and enhanced resource allocation during growth particularly under low phosphate (LP) conditions. Therefore, it can be concluded that the efficiency of the nodules via an increased allocation of resources and P acquiring mechanisms in V. divaricata may be the key to the plant’s ability to adapt to poor P environments and thus sustaining its reliance on BNF. From the data obtained as well as previous findings, it has been established that the phosphate conservation mechanisms in roots and nodules, involve the non-adenylate requiring PEPC-bypass route. 13C Nuclear magnetic resonance (NMR) gave us a better understanding regarding the… Advisors/Committee Members: Valentine, Alexander, Steenkamp, Emma, Stellenbosch University. Faculty of Science. Dept. of Botany and Zoology..

Subjects/Keywords: Plant metabolism; Phosphoenolpyruvate (PEP) metabolism; Phosphoenolpyruvate Carboxylase (PEPC); Virgilia divaricata – Cape Floristic Region; UCTD

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Stevens, G. (2015). The regulation of Phosphoenolpyruvate (PEP) metabolism via Phosphoenolpyruvate Carboxylase (PEPC) in P-deficient roots and nodules of Virgilia divaricata. (Doctoral Dissertation). Stellenbosch University. Retrieved from http://hdl.handle.net/10019.1/97853

Chicago Manual of Style (16^th Edition):

Stevens, Gary. “The regulation of Phosphoenolpyruvate (PEP) metabolism via Phosphoenolpyruvate Carboxylase (PEPC) in P-deficient roots and nodules of Virgilia divaricata.” 2015. Doctoral Dissertation, Stellenbosch University. Accessed April 13, 2021. http://hdl.handle.net/10019.1/97853.

MLA Handbook (7^th Edition):

Stevens, Gary. “The regulation of Phosphoenolpyruvate (PEP) metabolism via Phosphoenolpyruvate Carboxylase (PEPC) in P-deficient roots and nodules of Virgilia divaricata.” 2015. Web. 13 Apr 2021.

Vancouver:

Stevens G. The regulation of Phosphoenolpyruvate (PEP) metabolism via Phosphoenolpyruvate Carboxylase (PEPC) in P-deficient roots and nodules of Virgilia divaricata. [Internet] [Doctoral dissertation]. Stellenbosch University; 2015. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/10019.1/97853.

Council of Science Editors:

Stevens G. The regulation of Phosphoenolpyruvate (PEP) metabolism via Phosphoenolpyruvate Carboxylase (PEPC) in P-deficient roots and nodules of Virgilia divaricata. [Doctoral Dissertation]. Stellenbosch University; 2015. Available from: http://hdl.handle.net/10019.1/97853

University of Hyderabad

19. Murmu, Jhadeswar. Regulation of NADP malic enzyme and phosphoenolpyruvate carboxylase in leaves of C4 plants; -.

Degree: Plant sciences, 2004, University of Hyderabad

URL: http://shodhganga.inflibnet.ac.in/handle/10603/25693

None

Appendix p.157-163

Advisors/Committee Members: Raghavendra, A S.

Subjects/Keywords: NADP-malic enzyme; Phosphoenolpyruvate; Carboxylase

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Murmu, J. (2004). Regulation of NADP malic enzyme and phosphoenolpyruvate carboxylase in leaves of C4 plants; -. (Thesis). University of Hyderabad. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/25693

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Murmu, Jhadeswar. “Regulation of NADP malic enzyme and phosphoenolpyruvate carboxylase in leaves of C4 plants; -.” 2004. Thesis, University of Hyderabad. Accessed April 13, 2021. http://shodhganga.inflibnet.ac.in/handle/10603/25693.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Murmu, Jhadeswar. “Regulation of NADP malic enzyme and phosphoenolpyruvate carboxylase in leaves of C4 plants; -.” 2004. Web. 13 Apr 2021.

Vancouver:

Murmu J. Regulation of NADP malic enzyme and phosphoenolpyruvate carboxylase in leaves of C4 plants; -. [Internet] [Thesis]. University of Hyderabad; 2004. [cited 2021 Apr 13]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/25693.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Murmu J. Regulation of NADP malic enzyme and phosphoenolpyruvate carboxylase in leaves of C4 plants; -. [Thesis]. University of Hyderabad; 2004. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/25693

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Lund

20. Krus, Ulrika. Coupling mechanisms of insulin secretion - roles of mitochondrial metabolism and cAMP.

Degree: 2006, University of Lund

URL: https://lup.lub.lu.se/record/547657 ; https://portal.research.lu.se/ws/files/4540800/547659.pdf

► Type 2 diabetes is a disease that increases tremendously in the western world. It is characterized by insulin resistance and defects in insulin secretion. Insulin… (more)

Subjects/Keywords: Basic Medicine; secreting systems; Endocrinology; pyruvate dehydrogenase kinase; beta-cells; pyruvate carboxylase; diabetology; Endokrinologi; sekretion; diabetologi; diabetes

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Krus, U. (2006). Coupling mechanisms of insulin secretion - roles of mitochondrial metabolism and cAMP. (Doctoral Dissertation). University of Lund. Retrieved from https://lup.lub.lu.se/record/547657 ; https://portal.research.lu.se/ws/files/4540800/547659.pdf

Chicago Manual of Style (16^th Edition):

Krus, Ulrika. “Coupling mechanisms of insulin secretion - roles of mitochondrial metabolism and cAMP.” 2006. Doctoral Dissertation, University of Lund. Accessed April 13, 2021. https://lup.lub.lu.se/record/547657 ; https://portal.research.lu.se/ws/files/4540800/547659.pdf.

MLA Handbook (7^th Edition):

Krus, Ulrika. “Coupling mechanisms of insulin secretion - roles of mitochondrial metabolism and cAMP.” 2006. Web. 13 Apr 2021.

Vancouver:

Krus U. Coupling mechanisms of insulin secretion - roles of mitochondrial metabolism and cAMP. [Internet] [Doctoral dissertation]. University of Lund; 2006. [cited 2021 Apr 13]. Available from: https://lup.lub.lu.se/record/547657 ; https://portal.research.lu.se/ws/files/4540800/547659.pdf.

Council of Science Editors:

Krus U. Coupling mechanisms of insulin secretion - roles of mitochondrial metabolism and cAMP. [Doctoral Dissertation]. University of Lund; 2006. Available from: https://lup.lub.lu.se/record/547657 ; https://portal.research.lu.se/ws/files/4540800/547659.pdf

Universidade Estadual de Campinas

21. Tofanello, Vanessa Regina, 1982-. Influência do nitrogênio na capacidade fotossintética de cana-de-açúcar.

Degree: Instituto de Biologia; Programa de Pós-Graduação em Biologia Vegetal, 2020, Universidade Estadual de Campinas

URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/350985

►

Orientador: Rafael Vasconcelos Ribeiro

Tese (doutorado) - Universidade Estadual de Campinas, Instituto de Biologia

Made available in DSpace on 2020-10-14T10:57:23Z (GMT). No. of bitstreams: 1… (more)

▼

Orientador: Rafael Vasconcelos Ribeiro

Tese (doutorado) - Universidade Estadual de Campinas, Instituto de Biologia

Made available in DSpace on 2020-10-14T10:57:23Z (GMT). No. of bitstreams: 1 Tofanello_VanessaRegina_D.pdf: 1853921 bytes, checksum: a4ba3f22d73eea083e2909bb4d5f1038 (MD5) Previous issue date: 2020

Resumo: As plantas C4 evoluíram com um complexo mecanismo de concentração de CO2 (CCM) que envolve alterações bioquímica e anatômicas, que possibilitou melhoria na assimilação de CO2 sob baixa concentração de CO2 e baixa disponibilidade de água. Na literatura, há evidência de que a redução da fotossíntese de cana-de-açúcar sob baixo suprimento de nitrogênio é ocasionada pela redução da eficiência quântica da assimilação de CO2, aumento do vazamento de CO2 das células da bainha para as células do mesofilo e, portanto, a ineficiência do CCM. Porém estudos mais recentes constataram que o baixo suprimento de nitrogênio reduz a condutância das células da bainha do feixe vascular (gbs) ao CO2 em plantas de milho. Neste contexto, o objetivo foi testar a hipótese de que o baixo suprimento de nitrogênio reduziria o gbs, evitando reduções na eficiência do CCM em cana-de-açúcar. Plantas de cana da variedade IACSP95-5000 foram cultivadas sob cinco níveis de nitrogênio (0, 125, 250, 500 e 1000 mg N kg-1 de solo). Foram realizadas medidas combinadas de trocas gasosas e fluorescência da clorofila sob alta e baixa concentração de O2 no ar e variando a intensidade luminosa e a concentração de CO2 no ar para estimar a condutância das células da bainha do feixe vascular (gbs), condutância do mesofilo (gm), taxa de produção de ATP impulsionada pelo transporte de elétrons (Jatp), concentração de CO2 nos sítios de carboxilação da Rubisco (Cc), condutância do mesofilo (Cm), taxa máxima de carboxilação da Rubisco (Vcmax), taxa máxima de carboxilação da PEPC (Vpmax), vazamento de CO2 das células da bainha para as células do mesofilo (Ø) e a eficiência quântica da assimilação de CO2 (ØCO2). Também foram avaliadas características anatômicas das células da bainha do feixe e do mesofilo, expressões das aquaporinas ShPIP2;1 e ShPIP1;2 e abundância da Rubisco e PEPC. Encontramos correlação linear e positiva entre a concentração de nitrogênio nas folhas e a assimilação de CO2. O vazamento de CO2 das células da bainha para as células do mesofilo foi reduzido sob baixo suprimento de nitrogênio, juntamente com a redução do gbs. A atividade de carboxilação e a abundância da PEPC e Rubisco foram maiores sob alta oferta de nitrogênio, sendo Vpmax mais sensível à redução do nitrogênio foliar (LNC) quando comparado ao Vcmax, portanto ocasionando menor bombeamento de CO2 para células da bainha. No entanto, o baixo gbs estaria compensando as reduções no bombeamento de CO2 para células da bainha e assim o principal comprometimento metabólico ocasionado pela baixa disponibilidade de nitrogênio seria a baixa atividade de carboxilação da Rubisco. A expressão do gene ShPIP2;1 foi menor em alto nitrogênio foliar e, portanto, não explica a redução…

Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS, Ribeiro, Rafael Vasconcelos, 1977-, Machado, Eduardo Caruso, Andrade, Sara Adrián López de, Habermann, Gustavo, Oliveira, Ricardo Ferraz de.

Subjects/Keywords: Difusão; Células da bainha do feixe; Ribulose-bifosfato carboxilase; Fosfoenolpiruvato carboxilase; Diffusion; Bundle sheath cells; Ribulose-bisphosphate carboxylase; Phosphoenolpyruvate carboxylase

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Tofanello, Vanessa Regina, 1. (2020). Influência do nitrogênio na capacidade fotossintética de cana-de-açúcar. (Doctoral Dissertation). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/350985

Chicago Manual of Style (16^th Edition):

Tofanello, Vanessa Regina, 1982-. “Influência do nitrogênio na capacidade fotossintética de cana-de-açúcar.” 2020. Doctoral Dissertation, Universidade Estadual de Campinas. Accessed April 13, 2021. http://repositorio.unicamp.br/jspui/handle/REPOSIP/350985.

MLA Handbook (7^th Edition):

Tofanello, Vanessa Regina, 1982-. “Influência do nitrogênio na capacidade fotossintética de cana-de-açúcar.” 2020. Web. 13 Apr 2021.

Vancouver:

Tofanello, Vanessa Regina 1. Influência do nitrogênio na capacidade fotossintética de cana-de-açúcar. [Internet] [Doctoral dissertation]. Universidade Estadual de Campinas; 2020. [cited 2021 Apr 13]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/350985.

Council of Science Editors:

Tofanello, Vanessa Regina 1. Influência do nitrogênio na capacidade fotossintética de cana-de-açúcar. [Doctoral Dissertation]. Universidade Estadual de Campinas; 2020. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/350985

22. 이, 은하. Biochemical and molecular genetic studies on the pyruvate dehydrogenase complex and respiratory chain complex I in the patients with neurodegenerative disorders.

Degree: 2002, Ajou University

URL: http://repository.ajou.ac.kr/handle/201003/2141 ; http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000007955

Master Advisors/Committee Members: 대학원 의학과, 200024433, 이, 은하.

Subjects/Keywords: 퇴행성 신경질환; Pyruvate dehydrogenase complex; 전자전달계 complex I; neurodegenerative disorders

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

이, �. (2002). Biochemical and molecular genetic studies on the pyruvate dehydrogenase complex and respiratory chain complex I in the patients with neurodegenerative disorders. (Thesis). Ajou University. Retrieved from http://repository.ajou.ac.kr/handle/201003/2141 ; http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000007955

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

이, 은하. “Biochemical and molecular genetic studies on the pyruvate dehydrogenase complex and respiratory chain complex I in the patients with neurodegenerative disorders.” 2002. Thesis, Ajou University. Accessed April 13, 2021. http://repository.ajou.ac.kr/handle/201003/2141 ; http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000007955.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

이, 은하. “Biochemical and molecular genetic studies on the pyruvate dehydrogenase complex and respiratory chain complex I in the patients with neurodegenerative disorders.” 2002. Web. 13 Apr 2021.

Vancouver:

이 �. Biochemical and molecular genetic studies on the pyruvate dehydrogenase complex and respiratory chain complex I in the patients with neurodegenerative disorders. [Internet] [Thesis]. Ajou University; 2002. [cited 2021 Apr 13]. Available from: http://repository.ajou.ac.kr/handle/201003/2141 ; http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000007955.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

이 �. Biochemical and molecular genetic studies on the pyruvate dehydrogenase complex and respiratory chain complex I in the patients with neurodegenerative disorders. [Thesis]. Ajou University; 2002. Available from: http://repository.ajou.ac.kr/handle/201003/2141 ; http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000007955

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Georgia

23. Ma, Yingjie. Overcoming acetate in recombinant protein production by metabolic engineering of Escherichia coli.

Degree: 2014, University of Georgia

URL: http://hdl.handle.net/10724/25873

► The primary goal of this research is to understand the physiological cause for overflow metabolism of E. coli in order to improve the yield and… (more)

Subjects/Keywords: Protein production, Acetate, Escherichia coli,; Pyruvate carboxylase; PEP synthase; Pyruvate oxidase; Fed-batch

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ma, Y. (2014). Overcoming acetate in recombinant protein production by metabolic engineering of Escherichia coli. (Thesis). University of Georgia. Retrieved from http://hdl.handle.net/10724/25873

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ma, Yingjie. “Overcoming acetate in recombinant protein production by metabolic engineering of Escherichia coli.” 2014. Thesis, University of Georgia. Accessed April 13, 2021. http://hdl.handle.net/10724/25873.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ma, Yingjie. “Overcoming acetate in recombinant protein production by metabolic engineering of Escherichia coli.” 2014. Web. 13 Apr 2021.

Vancouver:

Ma Y. Overcoming acetate in recombinant protein production by metabolic engineering of Escherichia coli. [Internet] [Thesis]. University of Georgia; 2014. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/10724/25873.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ma Y. Overcoming acetate in recombinant protein production by metabolic engineering of Escherichia coli. [Thesis]. University of Georgia; 2014. Available from: http://hdl.handle.net/10724/25873

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Brock University

24. Herbst, Eric. The Role of Pyruvate Dehydrogenase Kinase-4 in Post-Exercise Glycogen Recovery .

Degree: Applied Health Sciences Program, 2011, Brock University

URL: http://hdl.handle.net/10464/3397

► The pyruvate dehydrogenase (PDH) complex regulates the oxidation of carbohydrates in mammals. Decreased activation of PDH following exhaustive exercise may aid the resynthesis of glycogen… (more)

Subjects/Keywords: pyruvate dehydrogenase; glycogen; exercise recovery; PDK4

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Herbst, E. (2011). The Role of Pyruvate Dehydrogenase Kinase-4 in Post-Exercise Glycogen Recovery . (Thesis). Brock University. Retrieved from http://hdl.handle.net/10464/3397

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Herbst, Eric. “The Role of Pyruvate Dehydrogenase Kinase-4 in Post-Exercise Glycogen Recovery .” 2011. Thesis, Brock University. Accessed April 13, 2021. http://hdl.handle.net/10464/3397.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Herbst, Eric. “The Role of Pyruvate Dehydrogenase Kinase-4 in Post-Exercise Glycogen Recovery .” 2011. Web. 13 Apr 2021.

Vancouver:

Herbst E. The Role of Pyruvate Dehydrogenase Kinase-4 in Post-Exercise Glycogen Recovery . [Internet] [Thesis]. Brock University; 2011. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/10464/3397.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Herbst E. The Role of Pyruvate Dehydrogenase Kinase-4 in Post-Exercise Glycogen Recovery . [Thesis]. Brock University; 2011. Available from: http://hdl.handle.net/10464/3397

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

25. Choptiany, Jonathan Robert. Phosphorylation of Skeletal Muscle Pyruvate Dehydrogenase Phosphatase in Response to Insulin Stimulation .

Degree: Applied Health Sciences Program, 2013, Brock University

URL: http://hdl.handle.net/10464/4722

► Pyruvate dehydrogenase phosphatase (PDP) regulates carbohydrate oxidation through the pyruvate dehydrogenase (PDH) complex. PDP activates PDH, enabling increased carbohydrate flux towards oxidative energy production. In… (more)

Subjects/Keywords: Pyruvate Dehydrogenase Complex; Pyruvate Dehydrogenase Phosphatase; Skeletal Muscle Metabolism; Insulin Stimulation

…perchloric acid PDH pyruvate dehydrogenase complex PDHa pyruvate dehydrogenase complex (… …used at different rates. The role of the active pyruvate dehydrogenase complex (PDHa… …E1 pyruvate dehydrogenase xii M.Sc. Thesis – J. R. Choptiany Brock University E1α… …pyruvate dehydrogenase alpha–subunit E2 dihydrolipoyl transacetylase E3 dihydrolipoyl… …active form) PDK pyruvate dehydrogenase kinase PDP pyruvate dehydrogenase phosphatase…

10 more images…

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Choptiany, J. R. (2013). Phosphorylation of Skeletal Muscle Pyruvate Dehydrogenase Phosphatase in Response to Insulin Stimulation . (Thesis). Brock University. Retrieved from http://hdl.handle.net/10464/4722

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Choptiany, Jonathan Robert. “Phosphorylation of Skeletal Muscle Pyruvate Dehydrogenase Phosphatase in Response to Insulin Stimulation .” 2013. Thesis, Brock University. Accessed April 13, 2021. http://hdl.handle.net/10464/4722.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Choptiany, Jonathan Robert. “Phosphorylation of Skeletal Muscle Pyruvate Dehydrogenase Phosphatase in Response to Insulin Stimulation .” 2013. Web. 13 Apr 2021.

Vancouver:

Choptiany JR. Phosphorylation of Skeletal Muscle Pyruvate Dehydrogenase Phosphatase in Response to Insulin Stimulation . [Internet] [Thesis]. Brock University; 2013. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/10464/4722.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Choptiany JR. Phosphorylation of Skeletal Muscle Pyruvate Dehydrogenase Phosphatase in Response to Insulin Stimulation . [Thesis]. Brock University; 2013. Available from: http://hdl.handle.net/10464/4722

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Toronto

26. Wu, Lu-Tse. Non-enzymic Fluoride Elimination and Iodination of the Breslow Intermediate.

Degree: 2017, University of Toronto

URL: http://hdl.handle.net/1807/79102

►

3-fluoropyruvate is an unusual substrate for pyruvate decarboxylase and pyruvate dehydrogenase that results in mechanistic divergence through exclusive fluoride elimination. A synthetic analogue, 2-(1-hydroxytrifluoroethyl)-thiamin (HTFETh),… (more)

Subjects/Keywords: Breslow intermediate; Flouride elimination; Pyruvate decarboxylase; Pyruvate dehydrogenase; Thiamin; 0485

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wu, L. (2017). Non-enzymic Fluoride Elimination and Iodination of the Breslow Intermediate. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/79102

Chicago Manual of Style (16^th Edition):

Wu, Lu-Tse. “Non-enzymic Fluoride Elimination and Iodination of the Breslow Intermediate.” 2017. Masters Thesis, University of Toronto. Accessed April 13, 2021. http://hdl.handle.net/1807/79102.

MLA Handbook (7^th Edition):

Wu, Lu-Tse. “Non-enzymic Fluoride Elimination and Iodination of the Breslow Intermediate.” 2017. Web. 13 Apr 2021.

Vancouver:

Wu L. Non-enzymic Fluoride Elimination and Iodination of the Breslow Intermediate. [Internet] [Masters thesis]. University of Toronto; 2017. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/1807/79102.

Council of Science Editors:

Wu L. Non-enzymic Fluoride Elimination and Iodination of the Breslow Intermediate. [Masters Thesis]. University of Toronto; 2017. Available from: http://hdl.handle.net/1807/79102

University of Adelaide

27. Ashman, Leonie Kay. Pyruvate carboxylase : reaction mechanism and chemical studies of the active centre.

Degree: 1973, University of Adelaide

URL: http://hdl.handle.net/2440/115036

Subjects/Keywords: Pyruvate carboxylase; Binding sites (Biochemistry)

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ashman, L. K. (1973). Pyruvate carboxylase : reaction mechanism and chemical studies of the active centre. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/115036

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ashman, Leonie Kay. “Pyruvate carboxylase : reaction mechanism and chemical studies of the active centre.” 1973. Thesis, University of Adelaide. Accessed April 13, 2021. http://hdl.handle.net/2440/115036.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ashman, Leonie Kay. “Pyruvate carboxylase : reaction mechanism and chemical studies of the active centre.” 1973. Web. 13 Apr 2021.

Vancouver:

Ashman LK. Pyruvate carboxylase : reaction mechanism and chemical studies of the active centre. [Internet] [Thesis]. University of Adelaide; 1973. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/2440/115036.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ashman LK. Pyruvate carboxylase : reaction mechanism and chemical studies of the active centre. [Thesis]. University of Adelaide; 1973. Available from: http://hdl.handle.net/2440/115036

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Columbia University

28. Choi, Philip H. Structural and functional studies on the regulation of pyruvate carboxylase by the bacterial second messenger cyclic-di-AMP.

Degree: 2017, Columbia University

URL: https://doi.org/10.7916/D8542T9P

► The primary focus of this dissertation is the metabolic enzyme pyruvate carboxylase (PC). The structure and function of this fascinating enzyme has been studies and… (more)

▼ The primary focus of this dissertation is the metabolic enzyme pyruvate carboxylase (PC). The structure and function of this fascinating enzyme has been studies and characterized by many laboratories over many decades. This extensive background is reviewed in Chapter 1, with an overview of the biotin-dependent carboxylase family and a particular focus on PC. In this dissertation, we primarily use X-ray crystallography to study PC at a structural level. This dissertation is divided into two overarching sections, with the first section (Chapters 2-5) focusing on the bacterial second messenger cyclic-di-AMP (c-di-AMP). This project was initiated by our collaborators in the laboratory of Josh Woodward at the University of Washington, who performed the first screen to identify c-di-AMP binding proteins in the bacterium Listeria monocytogenes. In Chapters 2 and 3, the regulation of PC by c-di-AMP in L. monocytogenes as well as the bacterium Lactococcus lactis is discussed. Crystal structures of the PC from each of these species in complex with cyclic-di-AMP reveal the binding site and give insights into the molecular mechanisms of this regulation. In Chapters 4 and 5, structural studies of other c-di-AMP binding proteins identified in the screen are discussed. The second section (Chapters 6) focuses on a second class of PC enzymes called the two-subunit PCs, which are found in a subset of Gram-negative bacteria. In Chapter 6, the first crystal structure of a two-subunit PC from the bacterium Methylobacillus flagellatus is determined. In collaboration with the Lars Dietrich laboratory at Columbia University, we investigate the physiological function of the two-subunit PC in the pathogen Pseudomonas aeruginosa. A theme which emerges from these studies is that PC is an incredibly diverse enzyme which has been adapted for the peculiar physiological needs of each organism it inhabits. Because PC is found throughout nature in every kingdom of life, further studies of its unique properties and role in each organism are sure to provide more surprising insights in the years to come.

Subjects/Keywords: Enzymes – Structure; Listeria monocytogenes; Enzymes; Pyruvate carboxylase; Biochemistry; Microbiology

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Choi, P. H. (2017). Structural and functional studies on the regulation of pyruvate carboxylase by the bacterial second messenger cyclic-di-AMP. (Doctoral Dissertation). Columbia University. Retrieved from https://doi.org/10.7916/D8542T9P

Chicago Manual of Style (16^th Edition):

Choi, Philip H. “Structural and functional studies on the regulation of pyruvate carboxylase by the bacterial second messenger cyclic-di-AMP.” 2017. Doctoral Dissertation, Columbia University. Accessed April 13, 2021. https://doi.org/10.7916/D8542T9P.

MLA Handbook (7^th Edition):

Choi, Philip H. “Structural and functional studies on the regulation of pyruvate carboxylase by the bacterial second messenger cyclic-di-AMP.” 2017. Web. 13 Apr 2021.

Vancouver:

Choi PH. Structural and functional studies on the regulation of pyruvate carboxylase by the bacterial second messenger cyclic-di-AMP. [Internet] [Doctoral dissertation]. Columbia University; 2017. [cited 2021 Apr 13]. Available from: https://doi.org/10.7916/D8542T9P.

Council of Science Editors:

Choi PH. Structural and functional studies on the regulation of pyruvate carboxylase by the bacterial second messenger cyclic-di-AMP. [Doctoral Dissertation]. Columbia University; 2017. Available from: https://doi.org/10.7916/D8542T9P

University of Adelaide

29. Cassady, Alan Ian. Pyruvate carboxylase : a molecular biological study / by Alan Ian Cassady.

Degree: 1987, University of Adelaide

URL: http://hdl.handle.net/2440/18646

Subjects/Keywords: 574.19254 20; Pyruvate carboxylase.

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Cassady, A. I. (1987). Pyruvate carboxylase : a molecular biological study / by Alan Ian Cassady. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/18646

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Cassady, Alan Ian. “Pyruvate carboxylase : a molecular biological study / by Alan Ian Cassady.” 1987. Thesis, University of Adelaide. Accessed April 13, 2021. http://hdl.handle.net/2440/18646.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Cassady, Alan Ian. “Pyruvate carboxylase : a molecular biological study / by Alan Ian Cassady.” 1987. Web. 13 Apr 2021.

Vancouver:

Cassady AI. Pyruvate carboxylase : a molecular biological study / by Alan Ian Cassady. [Internet] [Thesis]. University of Adelaide; 1987. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/2440/18646.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Cassady AI. Pyruvate carboxylase : a molecular biological study / by Alan Ian Cassady. [Thesis]. University of Adelaide; 1987. Available from: http://hdl.handle.net/2440/18646

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Adelaide

30. Booker, Grant William. Pyruvate carboxylase: relating structure to function / by Grant William Booker.

Degree: 1990, University of Adelaide

URL: http://hdl.handle.net/2440/19185

Subjects/Keywords: 574.1925 20; Pyruvate carboxylase.

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Booker, G. W. (1990). Pyruvate carboxylase: relating structure to function / by Grant William Booker. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/19185

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Booker, Grant William. “Pyruvate carboxylase: relating structure to function / by Grant William Booker.” 1990. Thesis, University of Adelaide. Accessed April 13, 2021. http://hdl.handle.net/2440/19185.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Booker, Grant William. “Pyruvate carboxylase: relating structure to function / by Grant William Booker.” 1990. Web. 13 Apr 2021.

Vancouver:

Booker GW. Pyruvate carboxylase: relating structure to function / by Grant William Booker. [Internet] [Thesis]. University of Adelaide; 1990. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/2440/19185.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Booker GW. Pyruvate carboxylase: relating structure to function / by Grant William Booker. [Thesis]. University of Adelaide; 1990. Available from: http://hdl.handle.net/2440/19185

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

		in
And / Or
		in
And / Or
		in
And / Or
		in

Open Access Theses and Dissertations