subject:(Pyruvate carboxylase)

University of Adelaide

1. Bais, Renze. Pyruvate carboxylase : physical and kinetic studies.

Degree: 1974, University of Adelaide

URL: http://hdl.handle.net/2440/19966

Subjects/Keywords: Pyruvate carboxylase

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APA (6^th Edition):

Bais, R. (1974). Pyruvate carboxylase : physical and kinetic studies. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/19966

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Bais, Renze. “Pyruvate carboxylase : physical and kinetic studies.” 1974. Thesis, University of Adelaide. Accessed January 27, 2021. http://hdl.handle.net/2440/19966.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Bais, Renze. “Pyruvate carboxylase : physical and kinetic studies.” 1974. Web. 27 Jan 2021.

Vancouver:

Bais R. Pyruvate carboxylase : physical and kinetic studies. [Internet] [Thesis]. University of Adelaide; 1974. [cited 2021 Jan 27]. Available from: http://hdl.handle.net/2440/19966.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Bais R. Pyruvate carboxylase : physical and kinetic studies. [Thesis]. University of Adelaide; 1974. Available from: http://hdl.handle.net/2440/19966

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Adelaide

2. Goss, Neil Howard. Pyruvate carboxylase: Physico-chemical aspects / a thesis submitted by Neil Howard Goss.

Degree: 1978, University of Adelaide

URL: http://hdl.handle.net/2440/20064

Subjects/Keywords: Pyruvate carboxylase

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APA (6^th Edition):

Goss, N. H. (1978). Pyruvate carboxylase: Physico-chemical aspects / a thesis submitted by Neil Howard Goss. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/20064

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Goss, Neil Howard. “Pyruvate carboxylase: Physico-chemical aspects / a thesis submitted by Neil Howard Goss.” 1978. Thesis, University of Adelaide. Accessed January 27, 2021. http://hdl.handle.net/2440/20064.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Goss, Neil Howard. “Pyruvate carboxylase: Physico-chemical aspects / a thesis submitted by Neil Howard Goss.” 1978. Web. 27 Jan 2021.

Vancouver:

Goss NH. Pyruvate carboxylase: Physico-chemical aspects / a thesis submitted by Neil Howard Goss. [Internet] [Thesis]. University of Adelaide; 1978. [cited 2021 Jan 27]. Available from: http://hdl.handle.net/2440/20064.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Goss NH. Pyruvate carboxylase: Physico-chemical aspects / a thesis submitted by Neil Howard Goss. [Thesis]. University of Adelaide; 1978. Available from: http://hdl.handle.net/2440/20064

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Adelaide

3. Phillips, Nelson Francis Boniface. Pyruvate carboxylase : studies on the reaction mechanism / by Nelson Francis Boniface Phillips.

Degree: 1981, University of Adelaide

URL: http://hdl.handle.net/2440/21134

Subjects/Keywords: Pyruvate carboxylase

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APA (6^th Edition):

Phillips, N. F. B. (1981). Pyruvate carboxylase : studies on the reaction mechanism / by Nelson Francis Boniface Phillips. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/21134

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Phillips, Nelson Francis Boniface. “Pyruvate carboxylase : studies on the reaction mechanism / by Nelson Francis Boniface Phillips.” 1981. Thesis, University of Adelaide. Accessed January 27, 2021. http://hdl.handle.net/2440/21134.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Phillips, Nelson Francis Boniface. “Pyruvate carboxylase : studies on the reaction mechanism / by Nelson Francis Boniface Phillips.” 1981. Web. 27 Jan 2021.

Vancouver:

Phillips NFB. Pyruvate carboxylase : studies on the reaction mechanism / by Nelson Francis Boniface Phillips. [Internet] [Thesis]. University of Adelaide; 1981. [cited 2021 Jan 27]. Available from: http://hdl.handle.net/2440/21134.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Phillips NFB. Pyruvate carboxylase : studies on the reaction mechanism / by Nelson Francis Boniface Phillips. [Thesis]. University of Adelaide; 1981. Available from: http://hdl.handle.net/2440/21134

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Adelaide

4. Easterbrook-Smith, Simon Barnett. The reaction mechanism of pyruvate carboxylase.

Degree: 1977, University of Adelaide

URL: http://hdl.handle.net/2440/37988

► 1. The 2 ', 3 ' -dialdehyde derivative of ATP (oATP) was prepared by periodate oxidation, and on the following criteria was considered to be… (more)

▼ 1. The 2 ', 3 ' -dialdehyde derivative of ATP (oATP) was prepared by periodate oxidation, and on the following criteria was considered to be an affinity label of the MgATP ²⁻ binding site of sheep liver pyruvate carboxylase. The magnesium complex of this inhibitor (Mg-oATP ² ⁻ ) was shown to be a linear competitive inhibitor with respect to MgATP ² ⁻ in both the acetyl CoA-dependent and -independent activities of the enzyme but was a non-competitive inhibitor with respect to bicarbonate and an un-competitive inhibitor with respect to pyruvate. Mg-oATP ² ⁻ was covalently bound to pyruvate carboxylase by reduction using sodium borohydride with concurrent irreversible inactivation of the enzyme. Although pyruvate, oxaloacetate, and bicarbonate were ineffective, both acetyl CoA and MgATP ² ⁻ protected the enzyme against this chemical modification. Mg ² ⁺ enhanced the extent of chemical modification. At 100% inactivation, 1.1 ± 0.1 moles of Mg-oATP ² ⁻ were bound to the enzyme per mole of biotin. The presence or absence of acetyl CoA had no affect on this stoichiometry. Chromatography of samples of an enzymic digest of Mg - o [¹ ⁴ C ] ATP ² ⁻ -labelled enzyme revealed one major band of radioactivity which co-chromatographed with authentic lys-oATP markers. 2. In order to further characterise the lysyl residue (s) in the biotin carboxylation site, the use of cyanate as a potential affinity label of the bicarbonate binding site was investigated. Cyanate was shown to chemically modify sheep liver pyruvate carboxylase, with concurrent inactivation of the enzyme. The rate of loss of acetyl CoA-independent catalytic activity was not pseudo first-order with respect to time. When the inactivation data were analysed assuming that modified enzyme retains some residual acetyl CoA-independent activity, it was found that only one kinetically significant residue per active site was modified, but cyanate appeared to form a reversible complex with the enzyme prior to modifying this residue. Acetyl CoA, pyruvate and oxaloacetate were found to have no affect on the rate of modification of the enzyme. Bicarbonate, MgADP, and MgATP ² ⁻ all afforded protection against inactivation of the enzyme. MgATP2 ⁻ afforded almost complete protection against loss of both the acetyl CoA-dependent and - independent catalytic activities. Analysis of the concentration dependence of protection by bicarbonate and MgATP ² ⁻ suggested that cyanate could not inactivate the enzyme - MgATP ² ⁻ and enzyme-bicarbonate complexes. The enzyme MgADP was found to be inactivated at about 30% of the rate of free enzyme. Modification of the enzyme with cyanate was found to inactivate the ATP/P [subscript i] isotopic exchange reaction, but to enhance the pyruvate/oxaloacetate isotopic exchange reaction. Inactivation of pyruvate carboxylase with [¹ ⁴ C ] cyanate was shown to be associated with modification of at least six amino acid residues per mole of biotin. Chromato-graphy of samples of an enzyme digest of [¹ ⁴ C ] cyanate-labelled enzyme revealed that… Advisors/Committee Members: Keech, Donald Bruce (advisor), Wallace, John Campbell (advisor), Dept. of Biochemistry (school).

Subjects/Keywords: pyruvate carboxylase

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APA (6^th Edition):

Easterbrook-Smith, S. B. (1977). The reaction mechanism of pyruvate carboxylase. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/37988

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Easterbrook-Smith, Simon Barnett. “The reaction mechanism of pyruvate carboxylase.” 1977. Thesis, University of Adelaide. Accessed January 27, 2021. http://hdl.handle.net/2440/37988.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Easterbrook-Smith, Simon Barnett. “The reaction mechanism of pyruvate carboxylase.” 1977. Web. 27 Jan 2021.

Vancouver:

Easterbrook-Smith SB. The reaction mechanism of pyruvate carboxylase. [Internet] [Thesis]. University of Adelaide; 1977. [cited 2021 Jan 27]. Available from: http://hdl.handle.net/2440/37988.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Easterbrook-Smith SB. The reaction mechanism of pyruvate carboxylase. [Thesis]. University of Adelaide; 1977. Available from: http://hdl.handle.net/2440/37988

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

5. Burkett, Daniel John. Synthesis and Evaluation of Small Molecule Inhibitors of Pyruvate Carboxylase.

Degree: 2020, Marquette University

URL: https://epublications.marquette.edu/theses_open/604

► Through structure-based drug design (SBDD), a series of small molecules have been synthesized and evaluated for inhibitory activity against the pyruvate carboxylase (PC) enzyme via… (more)

Subjects/Keywords: Hydroxycinnamic acids; Pyruvate carboxylase; Chemistry

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APA (6^th Edition):

Burkett, D. J. (2020). Synthesis and Evaluation of Small Molecule Inhibitors of Pyruvate Carboxylase. (Thesis). Marquette University. Retrieved from https://epublications.marquette.edu/theses_open/604

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Burkett, Daniel John. “Synthesis and Evaluation of Small Molecule Inhibitors of Pyruvate Carboxylase.” 2020. Thesis, Marquette University. Accessed January 27, 2021. https://epublications.marquette.edu/theses_open/604.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Burkett, Daniel John. “Synthesis and Evaluation of Small Molecule Inhibitors of Pyruvate Carboxylase.” 2020. Web. 27 Jan 2021.

Vancouver:

Burkett DJ. Synthesis and Evaluation of Small Molecule Inhibitors of Pyruvate Carboxylase. [Internet] [Thesis]. Marquette University; 2020. [cited 2021 Jan 27]. Available from: https://epublications.marquette.edu/theses_open/604.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Burkett DJ. Synthesis and Evaluation of Small Molecule Inhibitors of Pyruvate Carboxylase. [Thesis]. Marquette University; 2020. Available from: https://epublications.marquette.edu/theses_open/604

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Louisville

6. Sellers, Katherine. Pyruvate carboxylase is upregulated in NSCLC to support proliferation and anabolism.

Degree: PhD, 2013, University of Louisville

URL: 10.18297/etd/1297 ; https://ir.library.louisville.edu/etd/1297

► Non-small cell lung cancer (NSCLC) is the number one cause of cancer-related death and, like all cancers, requires metabolic reprogramming to support the energetic… (more)

▼ Non-small cell lung cancer (NSCLC) is the number one cause of cancer-related death and, like all cancers, requires metabolic reprogramming to support the energetic and biosynthetic demands of the tumor cells to proliferate and survive the tumor microenvironment. This dissertation sought to uncover novel metabolic dysfunctions in NSCLC that may lead to new, targeted therapeutic strategies. To this end, a stable isotope-resolved metabolomics approach was used to study NSCLC in human patients in vivo, in cell culture, and in mouse xenograft tumors. This approach involves treating biological samples with tracers containing stable isotopes, such as 13C6-glucose, and monitoring the incorporation of the isotope into metabolites by mass spectrometry and nuclear magnetic resonance spectroscopy. In Chapter 2, NSCLC-associated metabolic dysfunctions were elucidated by infusing human NSCLC patients with 13C6-glucose prior to tumor resection. Metabolite concentrations and labeling patterns in tumor tissues were compared to surrounding benign lung tissue from the same patient. The major findings were that tumor tissues had enhanced flux through glycolysis, the pentose phosphate pathway, glycogen synthesis and utilization, pyruvate dehydrogenase, malic enzyme, nucleotide biosynthesis, glycine biosynthesis, and UDP-GlcNAc biosynthesis. Particularly interesting was the increased rate of glucose oxidation through the TCA cycle, accompanied by increased utilization of TCA cycle intermediates for biosynthesis. To support this increase in TCA cycle flux, the expression and activity of the anapleurotic enzyme pyruvate carboxylase (PC) was enhanced in human NSCLC tumors. In Chapter 3, PC expression was suppressed in NSCLC cell lines by shRNA. The phenotypic and metabolic consequences were monitored in vitro and in mouse xenografts. Silencing PC reduced NSCLC cell growth, tumorigenicity, and survival. It was demonstrated that PC serves several important functions in NSCLC, including supporting mitochondrial activity, macromolecule biosynthesis especially lipids, and maintaining the redox status of the cell. In Chapter 4, small molecules were employed to inhibit PC activity in vitro, including a known anticancer agent methylseleninic acid (MSA). This is the first report demonstrating that MSA inhibits PC activity in vitro and in cell culture. Several other molecules with anti-PC activity were elucidated by in silico screening. Like PC knockdown, these inhibitors reduced NSCLC cell growth, tumorigenicity, and macromolecule biosynthesis. Together, these data indicate that PC is upregulated in NSCLC and functions to support anabolic processes critical to NSCLC proliferation and survival. Advisors/Committee Members: Fan, Teresa.

Subjects/Keywords: Cancer; Metabolism; Pyruvate carboxylase

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APA (6^th Edition):

Sellers, K. (2013). Pyruvate carboxylase is upregulated in NSCLC to support proliferation and anabolism. (Doctoral Dissertation). University of Louisville. Retrieved from 10.18297/etd/1297 ; https://ir.library.louisville.edu/etd/1297

Chicago Manual of Style (16^th Edition):

Sellers, Katherine. “Pyruvate carboxylase is upregulated in NSCLC to support proliferation and anabolism.” 2013. Doctoral Dissertation, University of Louisville. Accessed January 27, 2021. 10.18297/etd/1297 ; https://ir.library.louisville.edu/etd/1297.

MLA Handbook (7^th Edition):

Sellers, Katherine. “Pyruvate carboxylase is upregulated in NSCLC to support proliferation and anabolism.” 2013. Web. 27 Jan 2021.

Vancouver:

Sellers K. Pyruvate carboxylase is upregulated in NSCLC to support proliferation and anabolism. [Internet] [Doctoral dissertation]. University of Louisville; 2013. [cited 2021 Jan 27]. Available from: 10.18297/etd/1297 ; https://ir.library.louisville.edu/etd/1297.

Council of Science Editors:

Sellers K. Pyruvate carboxylase is upregulated in NSCLC to support proliferation and anabolism. [Doctoral Dissertation]. University of Louisville; 2013. Available from: 10.18297/etd/1297 ; https://ir.library.louisville.edu/etd/1297

University of Georgia

7. Tomar, Atin. Production of pyruvate by Escherichia coli using metabolic engineering.

Degree: 2014, University of Georgia

URL: http://hdl.handle.net/10724/29445

► Pyruvate is a three-carbon (triose) ketoacid central to metabolism that could potentially be produced by fermentation. Previous studies observed pyruvate accumulation in strains having mutations… (more)

Subjects/Keywords: Pyruvate; Escherichia coli; Acetate mutants; Pyruvate Dehydrogenase complex; Phosphoenolpyruvate carboxylase; Pyruvate oxidase; NADH oxidase

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Tomar, A. (2014). Production of pyruvate by Escherichia coli using metabolic engineering. (Thesis). University of Georgia. Retrieved from http://hdl.handle.net/10724/29445

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Tomar, Atin. “Production of pyruvate by Escherichia coli using metabolic engineering.” 2014. Thesis, University of Georgia. Accessed January 27, 2021. http://hdl.handle.net/10724/29445.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Tomar, Atin. “Production of pyruvate by Escherichia coli using metabolic engineering.” 2014. Web. 27 Jan 2021.

Vancouver:

Tomar A. Production of pyruvate by Escherichia coli using metabolic engineering. [Internet] [Thesis]. University of Georgia; 2014. [cited 2021 Jan 27]. Available from: http://hdl.handle.net/10724/29445.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Tomar A. Production of pyruvate by Escherichia coli using metabolic engineering. [Thesis]. University of Georgia; 2014. Available from: http://hdl.handle.net/10724/29445

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Virginia Tech

8. Kraszewski, Jessica. Enzymology and Physiology of a New Type of Phosphoenolpyruvate Carboxylase and the Development of a Pyruvate Carboxylase Expression System.

Degree: MS, Biochemistry, 2007, Virginia Tech

URL: http://hdl.handle.net/10919/40966

► Our laboratory is interested in studying the junction of glycolysis and the tricarboxylic acid (TCA) cycle, specifically the enzymes phosphoenolpyruvate carboxykinase, pyruvate carboxylase and phosphoenolpyruvate… (more)

▼ Our laboratory is interested in studying the junction of glycolysis and the tricarboxylic acid (TCA) cycle, specifically the enzymes phosphoenolpyruvate carboxykinase, pyruvate carboxylase and phosphoenolpyruvate carboxylase. All produce oxaloacetate (OAA) for the cell. OAA production is critical for cell carbon synthesis in the methanogenic archaea. Therefore OAA-generating enzymes are essential for the survival of methanogens. In part of this study we investigated archaeal-type phosphoenolpyruvate carboxylase (PpcA), a new type of phosphoenolpyruvate carboxylase, which is widespread in the archaea and is found in three bacterial species. The form of phosphoenolpyruvate carboxylase (Ppc) that is prevalent in bacteria and plants is not found in the archaea. Due to complications expressing PpcA in the soluble form and difficulty purifying this enzyme from methanogens, an in-depth investigation of this enzymeâ s biochemical properties has yet to occur. In this study we demonstrate the successful expression of a PpcA homolog in the soluble fraction of Escherichia coli. We purified the recombinant protein to homogeneity. This development provides the means to study the enzymeâ s biochemical properties and manipulate the primary sequence in order to identify residues critical to the enzymeâ s function. We also show that this PpcA homolog does have the postulated activity and investigate its biochemical properties. The data show that PpcA has unique properties in regard to the enzymeâ s substrate and its regulation by metabolites. Our data also reveal that PpcA is a membrane associated protein, unlike Ppc, which is a soluble protein. We also show that pyruvate carboxylase (Pyc) can be expressed recombinantly in Pseudomonas aeruginosa at levels sufficient for structure-function studies. This is a major step forward in the expression in Pyc because it cannot be expressed at high levels in Escherichia coli. These are important developments in studying the enzymes that connect glycolysis and the TCA cycle. Advisors/Committee Members: Mukhopadhyay, Biswarup (committeechair), Dean, Dennis R. (committee member), Kennelly, Peter J. (committee member), White, Robert H. (committee member), Stevens, Ann M. (committee member).

Subjects/Keywords: methanogens; pyruvate carboxylase; phosphoenolpyruvate carboxylase; enzymology

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APA (6^th Edition):

Kraszewski, J. (2007). Enzymology and Physiology of a New Type of Phosphoenolpyruvate Carboxylase and the Development of a Pyruvate Carboxylase Expression System. (Masters Thesis). Virginia Tech. Retrieved from http://hdl.handle.net/10919/40966

Chicago Manual of Style (16^th Edition):

Kraszewski, Jessica. “Enzymology and Physiology of a New Type of Phosphoenolpyruvate Carboxylase and the Development of a Pyruvate Carboxylase Expression System.” 2007. Masters Thesis, Virginia Tech. Accessed January 27, 2021. http://hdl.handle.net/10919/40966.

MLA Handbook (7^th Edition):

Kraszewski, Jessica. “Enzymology and Physiology of a New Type of Phosphoenolpyruvate Carboxylase and the Development of a Pyruvate Carboxylase Expression System.” 2007. Web. 27 Jan 2021.

Vancouver:

Kraszewski J. Enzymology and Physiology of a New Type of Phosphoenolpyruvate Carboxylase and the Development of a Pyruvate Carboxylase Expression System. [Internet] [Masters thesis]. Virginia Tech; 2007. [cited 2021 Jan 27]. Available from: http://hdl.handle.net/10919/40966.

Council of Science Editors:

Kraszewski J. Enzymology and Physiology of a New Type of Phosphoenolpyruvate Carboxylase and the Development of a Pyruvate Carboxylase Expression System. [Masters Thesis]. Virginia Tech; 2007. Available from: http://hdl.handle.net/10919/40966

University of Georgia

9. Ma, Yingjie. Overcoming acetate in recombinant protein production by metabolic engineering of Escherichia coli.

Degree: 2014, University of Georgia

URL: http://hdl.handle.net/10724/25873

► The primary goal of this research is to understand the physiological cause for overflow metabolism of E. coli in order to improve the yield and… (more)

Subjects/Keywords: Protein production, Acetate, Escherichia coli,; Pyruvate carboxylase; PEP synthase; Pyruvate oxidase; Fed-batch

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ma, Y. (2014). Overcoming acetate in recombinant protein production by metabolic engineering of Escherichia coli. (Thesis). University of Georgia. Retrieved from http://hdl.handle.net/10724/25873

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ma, Yingjie. “Overcoming acetate in recombinant protein production by metabolic engineering of Escherichia coli.” 2014. Thesis, University of Georgia. Accessed January 27, 2021. http://hdl.handle.net/10724/25873.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ma, Yingjie. “Overcoming acetate in recombinant protein production by metabolic engineering of Escherichia coli.” 2014. Web. 27 Jan 2021.

Vancouver:

Ma Y. Overcoming acetate in recombinant protein production by metabolic engineering of Escherichia coli. [Internet] [Thesis]. University of Georgia; 2014. [cited 2021 Jan 27]. Available from: http://hdl.handle.net/10724/25873.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ma Y. Overcoming acetate in recombinant protein production by metabolic engineering of Escherichia coli. [Thesis]. University of Georgia; 2014. Available from: http://hdl.handle.net/10724/25873

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Georgia

10. Yang, Fei. Production of pyruvate by Escherichia coli using metabolic engineering.

Degree: 2014, University of Georgia

URL: http://hdl.handle.net/10724/22034

► Pyruvate is a three-carbon ketoacid produced at the end of glycolysis. This research involves the metabolic engineering of the bacterium Escherichia coli in order to… (more)

Subjects/Keywords: Pyruvate; Escherichia coli; Pyruvate dehydrogenase complex, Phosphoenolpyruvate carboxylase; Pyruvate oxidase; NADH oxidase; 2,4-dinitrophenol, Plackett-Burman design

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APA (6^th Edition):

Yang, F. (2014). Production of pyruvate by Escherichia coli using metabolic engineering. (Thesis). University of Georgia. Retrieved from http://hdl.handle.net/10724/22034

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Yang, Fei. “Production of pyruvate by Escherichia coli using metabolic engineering.” 2014. Thesis, University of Georgia. Accessed January 27, 2021. http://hdl.handle.net/10724/22034.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Yang, Fei. “Production of pyruvate by Escherichia coli using metabolic engineering.” 2014. Web. 27 Jan 2021.

Vancouver:

Yang F. Production of pyruvate by Escherichia coli using metabolic engineering. [Internet] [Thesis]. University of Georgia; 2014. [cited 2021 Jan 27]. Available from: http://hdl.handle.net/10724/22034.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Yang F. Production of pyruvate by Escherichia coli using metabolic engineering. [Thesis]. University of Georgia; 2014. Available from: http://hdl.handle.net/10724/22034

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Adelaide

11. Ashman, Leonie Kay. Pyruvate carboxylase : reaction mechanism and chemical studies of the active centre.

Degree: 1973, University of Adelaide

URL: http://hdl.handle.net/2440/115036

Subjects/Keywords: Pyruvate carboxylase; Binding sites (Biochemistry)

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ashman, L. K. (1973). Pyruvate carboxylase : reaction mechanism and chemical studies of the active centre. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/115036

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ashman, Leonie Kay. “Pyruvate carboxylase : reaction mechanism and chemical studies of the active centre.” 1973. Thesis, University of Adelaide. Accessed January 27, 2021. http://hdl.handle.net/2440/115036.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ashman, Leonie Kay. “Pyruvate carboxylase : reaction mechanism and chemical studies of the active centre.” 1973. Web. 27 Jan 2021.

Vancouver:

Ashman LK. Pyruvate carboxylase : reaction mechanism and chemical studies of the active centre. [Internet] [Thesis]. University of Adelaide; 1973. [cited 2021 Jan 27]. Available from: http://hdl.handle.net/2440/115036.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ashman LK. Pyruvate carboxylase : reaction mechanism and chemical studies of the active centre. [Thesis]. University of Adelaide; 1973. Available from: http://hdl.handle.net/2440/115036

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Columbia University

12. Choi, Philip H. Structural and functional studies on the regulation of pyruvate carboxylase by the bacterial second messenger cyclic-di-AMP.

Degree: 2017, Columbia University

URL: https://doi.org/10.7916/D8542T9P

► The primary focus of this dissertation is the metabolic enzyme pyruvate carboxylase (PC). The structure and function of this fascinating enzyme has been studies and… (more)

▼ The primary focus of this dissertation is the metabolic enzyme pyruvate carboxylase (PC). The structure and function of this fascinating enzyme has been studies and characterized by many laboratories over many decades. This extensive background is reviewed in Chapter 1, with an overview of the biotin-dependent carboxylase family and a particular focus on PC. In this dissertation, we primarily use X-ray crystallography to study PC at a structural level. This dissertation is divided into two overarching sections, with the first section (Chapters 2-5) focusing on the bacterial second messenger cyclic-di-AMP (c-di-AMP). This project was initiated by our collaborators in the laboratory of Josh Woodward at the University of Washington, who performed the first screen to identify c-di-AMP binding proteins in the bacterium Listeria monocytogenes. In Chapters 2 and 3, the regulation of PC by c-di-AMP in L. monocytogenes as well as the bacterium Lactococcus lactis is discussed. Crystal structures of the PC from each of these species in complex with cyclic-di-AMP reveal the binding site and give insights into the molecular mechanisms of this regulation. In Chapters 4 and 5, structural studies of other c-di-AMP binding proteins identified in the screen are discussed. The second section (Chapters 6) focuses on a second class of PC enzymes called the two-subunit PCs, which are found in a subset of Gram-negative bacteria. In Chapter 6, the first crystal structure of a two-subunit PC from the bacterium Methylobacillus flagellatus is determined. In collaboration with the Lars Dietrich laboratory at Columbia University, we investigate the physiological function of the two-subunit PC in the pathogen Pseudomonas aeruginosa. A theme which emerges from these studies is that PC is an incredibly diverse enzyme which has been adapted for the peculiar physiological needs of each organism it inhabits. Because PC is found throughout nature in every kingdom of life, further studies of its unique properties and role in each organism are sure to provide more surprising insights in the years to come.

Subjects/Keywords: Enzymes – Structure; Listeria monocytogenes; Enzymes; Pyruvate carboxylase; Biochemistry; Microbiology

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APA (6^th Edition):

Choi, P. H. (2017). Structural and functional studies on the regulation of pyruvate carboxylase by the bacterial second messenger cyclic-di-AMP. (Doctoral Dissertation). Columbia University. Retrieved from https://doi.org/10.7916/D8542T9P

Chicago Manual of Style (16^th Edition):

Choi, Philip H. “Structural and functional studies on the regulation of pyruvate carboxylase by the bacterial second messenger cyclic-di-AMP.” 2017. Doctoral Dissertation, Columbia University. Accessed January 27, 2021. https://doi.org/10.7916/D8542T9P.

MLA Handbook (7^th Edition):

Choi, Philip H. “Structural and functional studies on the regulation of pyruvate carboxylase by the bacterial second messenger cyclic-di-AMP.” 2017. Web. 27 Jan 2021.

Vancouver:

Choi PH. Structural and functional studies on the regulation of pyruvate carboxylase by the bacterial second messenger cyclic-di-AMP. [Internet] [Doctoral dissertation]. Columbia University; 2017. [cited 2021 Jan 27]. Available from: https://doi.org/10.7916/D8542T9P.

Council of Science Editors:

Choi PH. Structural and functional studies on the regulation of pyruvate carboxylase by the bacterial second messenger cyclic-di-AMP. [Doctoral Dissertation]. Columbia University; 2017. Available from: https://doi.org/10.7916/D8542T9P

University of Adelaide

13. Cassady, Alan Ian. Pyruvate carboxylase : a molecular biological study / by Alan Ian Cassady.

Degree: 1987, University of Adelaide

URL: http://hdl.handle.net/2440/18646

Subjects/Keywords: 574.19254 20; Pyruvate carboxylase.

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APA (6^th Edition):

Cassady, A. I. (1987). Pyruvate carboxylase : a molecular biological study / by Alan Ian Cassady. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/18646

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Cassady, Alan Ian. “Pyruvate carboxylase : a molecular biological study / by Alan Ian Cassady.” 1987. Thesis, University of Adelaide. Accessed January 27, 2021. http://hdl.handle.net/2440/18646.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Cassady, Alan Ian. “Pyruvate carboxylase : a molecular biological study / by Alan Ian Cassady.” 1987. Web. 27 Jan 2021.

Vancouver:

Cassady AI. Pyruvate carboxylase : a molecular biological study / by Alan Ian Cassady. [Internet] [Thesis]. University of Adelaide; 1987. [cited 2021 Jan 27]. Available from: http://hdl.handle.net/2440/18646.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Cassady AI. Pyruvate carboxylase : a molecular biological study / by Alan Ian Cassady. [Thesis]. University of Adelaide; 1987. Available from: http://hdl.handle.net/2440/18646

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Adelaide

14. Booker, Grant William. Pyruvate carboxylase: relating structure to function / by Grant William Booker.

Degree: 1990, University of Adelaide

URL: http://hdl.handle.net/2440/19185

Subjects/Keywords: 574.1925 20; Pyruvate carboxylase.

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APA (6^th Edition):

Booker, G. W. (1990). Pyruvate carboxylase: relating structure to function / by Grant William Booker. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/19185

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Booker, Grant William. “Pyruvate carboxylase: relating structure to function / by Grant William Booker.” 1990. Thesis, University of Adelaide. Accessed January 27, 2021. http://hdl.handle.net/2440/19185.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Booker, Grant William. “Pyruvate carboxylase: relating structure to function / by Grant William Booker.” 1990. Web. 27 Jan 2021.

Vancouver:

Booker GW. Pyruvate carboxylase: relating structure to function / by Grant William Booker. [Internet] [Thesis]. University of Adelaide; 1990. [cited 2021 Jan 27]. Available from: http://hdl.handle.net/2440/19185.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Booker GW. Pyruvate carboxylase: relating structure to function / by Grant William Booker. [Thesis]. University of Adelaide; 1990. Available from: http://hdl.handle.net/2440/19185

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Adelaide

15. Jitrapakdee, Sarawut. Characterisation of the pyruvate carboxylase gene and studies on the regulation of its expression in rat / by Sarawut Jitrapakdee.

Degree: 1999, University of Adelaide

URL: http://hdl.handle.net/2440/19388

Subjects/Keywords: Pyruvate carboxylase Metabolism Genetic aspects.

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APA (6^th Edition):

Jitrapakdee, S. (1999). Characterisation of the pyruvate carboxylase gene and studies on the regulation of its expression in rat / by Sarawut Jitrapakdee. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/19388

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Jitrapakdee, Sarawut. “Characterisation of the pyruvate carboxylase gene and studies on the regulation of its expression in rat / by Sarawut Jitrapakdee.” 1999. Thesis, University of Adelaide. Accessed January 27, 2021. http://hdl.handle.net/2440/19388.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Jitrapakdee, Sarawut. “Characterisation of the pyruvate carboxylase gene and studies on the regulation of its expression in rat / by Sarawut Jitrapakdee.” 1999. Web. 27 Jan 2021.

Vancouver:

Jitrapakdee S. Characterisation of the pyruvate carboxylase gene and studies on the regulation of its expression in rat / by Sarawut Jitrapakdee. [Internet] [Thesis]. University of Adelaide; 1999. [cited 2021 Jan 27]. Available from: http://hdl.handle.net/2440/19388.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Jitrapakdee S. Characterisation of the pyruvate carboxylase gene and studies on the regulation of its expression in rat / by Sarawut Jitrapakdee. [Thesis]. University of Adelaide; 1999. Available from: http://hdl.handle.net/2440/19388

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Adelaide

16. Clements, Peter Roy. Pyruvate carboxylase : the mode of action of acetye CoA / a thesis submitted by Peter Roy Clements.

Degree: 1977, University of Adelaide

URL: http://hdl.handle.net/2440/20037

Subjects/Keywords: Pyruvate carboxylase; Acetylcoenzyme A.

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APA (6^th Edition):

Clements, P. R. (1977). Pyruvate carboxylase : the mode of action of acetye CoA / a thesis submitted by Peter Roy Clements. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/20037

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Clements, Peter Roy. “Pyruvate carboxylase : the mode of action of acetye CoA / a thesis submitted by Peter Roy Clements.” 1977. Thesis, University of Adelaide. Accessed January 27, 2021. http://hdl.handle.net/2440/20037.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Clements, Peter Roy. “Pyruvate carboxylase : the mode of action of acetye CoA / a thesis submitted by Peter Roy Clements.” 1977. Web. 27 Jan 2021.

Vancouver:

Clements PR. Pyruvate carboxylase : the mode of action of acetye CoA / a thesis submitted by Peter Roy Clements. [Internet] [Thesis]. University of Adelaide; 1977. [cited 2021 Jan 27]. Available from: http://hdl.handle.net/2440/20037.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Clements PR. Pyruvate carboxylase : the mode of action of acetye CoA / a thesis submitted by Peter Roy Clements. [Thesis]. University of Adelaide; 1977. Available from: http://hdl.handle.net/2440/20037

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Adelaide

17. Chapman-Smith, Anne. Studies on the interaction of sheep liver pyruvate carboxylase with the allosteric effector, acetyl CoA / by Anne Chapman-Smith.

Degree: 1981, University of Adelaide

URL: http://hdl.handle.net/2440/20687

Subjects/Keywords: Pyruvate carboxylase; Acetylcoenzyme A.

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Chapman-Smith, A. (1981). Studies on the interaction of sheep liver pyruvate carboxylase with the allosteric effector, acetyl CoA / by Anne Chapman-Smith. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/20687

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Chapman-Smith, Anne. “Studies on the interaction of sheep liver pyruvate carboxylase with the allosteric effector, acetyl CoA / by Anne Chapman-Smith.” 1981. Thesis, University of Adelaide. Accessed January 27, 2021. http://hdl.handle.net/2440/20687.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Chapman-Smith, Anne. “Studies on the interaction of sheep liver pyruvate carboxylase with the allosteric effector, acetyl CoA / by Anne Chapman-Smith.” 1981. Web. 27 Jan 2021.

Vancouver:

Chapman-Smith A. Studies on the interaction of sheep liver pyruvate carboxylase with the allosteric effector, acetyl CoA / by Anne Chapman-Smith. [Internet] [Thesis]. University of Adelaide; 1981. [cited 2021 Jan 27]. Available from: http://hdl.handle.net/2440/20687.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Chapman-Smith A. Studies on the interaction of sheep liver pyruvate carboxylase with the allosteric effector, acetyl CoA / by Anne Chapman-Smith. [Thesis]. University of Adelaide; 1981. Available from: http://hdl.handle.net/2440/20687

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Adelaide

18. Rylatt, Dennis Brian. The structure and mode of action of the biotinyl prosthetic group and its covalent attachment site in pyruvate carboxylase.

Degree: 1976, University of Adelaide

URL: http://hdl.handle.net/2440/21188

Subjects/Keywords: Vitamin H.; Pyruvate carboxylase.

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APA (6^th Edition):

Rylatt, D. B. (1976). The structure and mode of action of the biotinyl prosthetic group and its covalent attachment site in pyruvate carboxylase. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/21188

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Rylatt, Dennis Brian. “The structure and mode of action of the biotinyl prosthetic group and its covalent attachment site in pyruvate carboxylase.” 1976. Thesis, University of Adelaide. Accessed January 27, 2021. http://hdl.handle.net/2440/21188.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Rylatt, Dennis Brian. “The structure and mode of action of the biotinyl prosthetic group and its covalent attachment site in pyruvate carboxylase.” 1976. Web. 27 Jan 2021.

Vancouver:

Rylatt DB. The structure and mode of action of the biotinyl prosthetic group and its covalent attachment site in pyruvate carboxylase. [Internet] [Thesis]. University of Adelaide; 1976. [cited 2021 Jan 27]. Available from: http://hdl.handle.net/2440/21188.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Rylatt DB. The structure and mode of action of the biotinyl prosthetic group and its covalent attachment site in pyruvate carboxylase. [Thesis]. University of Adelaide; 1976. Available from: http://hdl.handle.net/2440/21188

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Adelaide

19. Taylor, Patricia Helen. Pyruvate carboxylase and pep carboxykinase in sheep.

Degree: 1971, University of Adelaide

URL: http://hdl.handle.net/2440/21305

Subjects/Keywords: PEP carboxykinase.; Pyruvate carboxylase.

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Taylor, P. H. (1971). Pyruvate carboxylase and pep carboxykinase in sheep. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/21305

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Taylor, Patricia Helen. “Pyruvate carboxylase and pep carboxykinase in sheep.” 1971. Thesis, University of Adelaide. Accessed January 27, 2021. http://hdl.handle.net/2440/21305.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Taylor, Patricia Helen. “Pyruvate carboxylase and pep carboxykinase in sheep.” 1971. Web. 27 Jan 2021.

Vancouver:

Taylor PH. Pyruvate carboxylase and pep carboxykinase in sheep. [Internet] [Thesis]. University of Adelaide; 1971. [cited 2021 Jan 27]. Available from: http://hdl.handle.net/2440/21305.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Taylor PH. Pyruvate carboxylase and pep carboxykinase in sheep. [Thesis]. University of Adelaide; 1971. Available from: http://hdl.handle.net/2440/21305

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Adelaide

20. Teerakul Arpornsuwan. Pyruvate carboxylase : a structure and function study using monoclonal antibodies and mutagenesis / Teerakul Arpornsuwan.

Degree: 2003, University of Adelaide

URL: http://hdl.handle.net/2440/22032

Subjects/Keywords: Pyruvate carboxylase Metabolism Molecular aspects.

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APA (6^th Edition):

Arpornsuwan, T. (2003). Pyruvate carboxylase : a structure and function study using monoclonal antibodies and mutagenesis / Teerakul Arpornsuwan. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/22032

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Arpornsuwan, Teerakul. “Pyruvate carboxylase : a structure and function study using monoclonal antibodies and mutagenesis / Teerakul Arpornsuwan.” 2003. Thesis, University of Adelaide. Accessed January 27, 2021. http://hdl.handle.net/2440/22032.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Arpornsuwan, Teerakul. “Pyruvate carboxylase : a structure and function study using monoclonal antibodies and mutagenesis / Teerakul Arpornsuwan.” 2003. Web. 27 Jan 2021.

Vancouver:

Arpornsuwan T. Pyruvate carboxylase : a structure and function study using monoclonal antibodies and mutagenesis / Teerakul Arpornsuwan. [Internet] [Thesis]. University of Adelaide; 2003. [cited 2021 Jan 27]. Available from: http://hdl.handle.net/2440/22032.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Arpornsuwan T. Pyruvate carboxylase : a structure and function study using monoclonal antibodies and mutagenesis / Teerakul Arpornsuwan. [Thesis]. University of Adelaide; 2003. Available from: http://hdl.handle.net/2440/22032

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

21. Liu, Yumeng. Allosteric Regulation of Pyruvate Carboxylase.

Degree: 2018, Marquette University

URL: https://epublications.marquette.edu/dissertations_mu/804

► Pyruvate carboxylase (PC; E.C.6.4.1.1) is a multifunctional, biotin-dependent enzyme that catalyzes the MgATP-dependent carboxylation of pyruvate to oxaloacetate. The overall reaction is accomplished by the… (more)

▼ Pyruvate carboxylase (PC; E.C.6.4.1.1) is a multifunctional, biotin-dependent enzyme that catalyzes the MgATP-dependent carboxylation of pyruvate to oxaloacetate. The overall reaction is accomplished by the coupling of two half reactions occurring at two spatially distinct catalytic domains by the translocation of a carrier domain, resulting in a net transfer of CO2 from bicarbonate to pyruvate. PC activity is regulated by multiple allosteric effectors with acetyl CoA serving as an activator in most species and L-aspartate serving as an inhibitor for microbial PC. The kinetic characterization of PC from different species have revealed that PC homologs are subject to divergent degrees of allosteric regulation, yet the mechanism controlling the allosteric response still remains unknown. Rhizobium etli (RePC) is allosterically activated by acetyl CoA, while Aspergillus nidulans (AnPC) is unique in not being activated by acetyl CoA. The divergent regulatory properties of these two PC homologs were investigated in order to uncover the fundamental mechanism underlying the allosteric regulation of pyruvate carboxylase. The lack of sensitivity to acetyl CoA activation was determined to be an intrinsic property of the BC domain rather than a consequence of different acetyl CoA binding sites. Further characterization of AnPC compared to RePC revealed that AnPC is a highly stable tetramer that remains in a permanently activated state. A more ordered transition state and coupled half reactions contribute to its high intrinsic catalytic rate as well as the insensitivity to the activation by acetyl CoA. Investigations on the translocation of the carrier domain revealed that multiple translocation pathways are adopted by the carrier domain in PC during catalysis. Interestingly, an intermolecular translocation pathway was promoted upon binding with acetyl CoA in RePC, which likely contributes to the enhanced coupling efficiency as well as the elevated catalytic rate observed in RePC. Finally, studies on the allosteric inhibition of PC by L-aspartate revealed that the antagonistic effects of acetyl CoA and L-aspartate to the catalysis of PC result from their competitive binding at a partially overlapping binding site. Collectively, these studies offer new insights into the mechanism of allosteric regulation in pyruvate carboxylase and provide an example of how divergent intrinsic properties in homologous enzymes can give rise to their unique regulatory properties. Advisors/Committee Members: Schläppi, Michael R., Maurice, Martin St., Silvaggi, Nicholas R..

Subjects/Keywords: acetyl coenzyme A; Allosteric regulation; Pyruvate carboxylase; Biochemistry

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APA (6^th Edition):

Liu, Y. (2018). Allosteric Regulation of Pyruvate Carboxylase. (Thesis). Marquette University. Retrieved from https://epublications.marquette.edu/dissertations_mu/804

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Liu, Yumeng. “Allosteric Regulation of Pyruvate Carboxylase.” 2018. Thesis, Marquette University. Accessed January 27, 2021. https://epublications.marquette.edu/dissertations_mu/804.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Liu, Yumeng. “Allosteric Regulation of Pyruvate Carboxylase.” 2018. Web. 27 Jan 2021.

Vancouver:

Liu Y. Allosteric Regulation of Pyruvate Carboxylase. [Internet] [Thesis]. Marquette University; 2018. [cited 2021 Jan 27]. Available from: https://epublications.marquette.edu/dissertations_mu/804.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Liu Y. Allosteric Regulation of Pyruvate Carboxylase. [Thesis]. Marquette University; 2018. Available from: https://epublications.marquette.edu/dissertations_mu/804

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Purdue University

22. Boesche, Katherine Elizabeth. Control of bovine Pyruvate Carboxylase Expression by Saturated and Unsaturated Fatty Acids and Impact on Fatty Acid Metabolism.

Degree: PhD, Animal Science, 2015, Purdue University

URL: https://docs.lib.purdue.edu/open_access_dissertations/1093

► Pyruvate carboxylase (PC; EC 6.4.1.1) ostensibly links carbohydrate and lipid metabolism by supplying oxaloacetate (OAA) from the carboxylation of pyruvate. The OAA pool is necessary… (more)

Subjects/Keywords: bovine; dairy cow; fatty acid; oxaloacetate; oxidation; pyruvate carboxylase

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APA (6^th Edition):

Boesche, K. E. (2015). Control of bovine Pyruvate Carboxylase Expression by Saturated and Unsaturated Fatty Acids and Impact on Fatty Acid Metabolism. (Doctoral Dissertation). Purdue University. Retrieved from https://docs.lib.purdue.edu/open_access_dissertations/1093

Chicago Manual of Style (16^th Edition):

Boesche, Katherine Elizabeth. “Control of bovine Pyruvate Carboxylase Expression by Saturated and Unsaturated Fatty Acids and Impact on Fatty Acid Metabolism.” 2015. Doctoral Dissertation, Purdue University. Accessed January 27, 2021. https://docs.lib.purdue.edu/open_access_dissertations/1093.

MLA Handbook (7^th Edition):

Boesche, Katherine Elizabeth. “Control of bovine Pyruvate Carboxylase Expression by Saturated and Unsaturated Fatty Acids and Impact on Fatty Acid Metabolism.” 2015. Web. 27 Jan 2021.

Vancouver:

Boesche KE. Control of bovine Pyruvate Carboxylase Expression by Saturated and Unsaturated Fatty Acids and Impact on Fatty Acid Metabolism. [Internet] [Doctoral dissertation]. Purdue University; 2015. [cited 2021 Jan 27]. Available from: https://docs.lib.purdue.edu/open_access_dissertations/1093.

Council of Science Editors:

Boesche KE. Control of bovine Pyruvate Carboxylase Expression by Saturated and Unsaturated Fatty Acids and Impact on Fatty Acid Metabolism. [Doctoral Dissertation]. Purdue University; 2015. Available from: https://docs.lib.purdue.edu/open_access_dissertations/1093

University of Adelaide

23. Khew-Goodall, Yeesim. Pyruvate carboxylase: its interactions with acetyl CoA / by Yee Sim Khew-Goodall.

Degree: 1985, University of Adelaide

URL: http://hdl.handle.net/2440/19886

Subjects/Keywords: 574.1925 19; Pyruvate carboxylase.; Acetylcoenzyme A.

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APA (6^th Edition):

Khew-Goodall, Y. (1985). Pyruvate carboxylase: its interactions with acetyl CoA / by Yee Sim Khew-Goodall. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/19886

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Khew-Goodall, Yeesim. “Pyruvate carboxylase: its interactions with acetyl CoA / by Yee Sim Khew-Goodall.” 1985. Thesis, University of Adelaide. Accessed January 27, 2021. http://hdl.handle.net/2440/19886.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Khew-Goodall, Yeesim. “Pyruvate carboxylase: its interactions with acetyl CoA / by Yee Sim Khew-Goodall.” 1985. Web. 27 Jan 2021.

Vancouver:

Khew-Goodall Y. Pyruvate carboxylase: its interactions with acetyl CoA / by Yee Sim Khew-Goodall. [Internet] [Thesis]. University of Adelaide; 1985. [cited 2021 Jan 27]. Available from: http://hdl.handle.net/2440/19886.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Khew-Goodall Y. Pyruvate carboxylase: its interactions with acetyl CoA / by Yee Sim Khew-Goodall. [Thesis]. University of Adelaide; 1985. Available from: http://hdl.handle.net/2440/19886

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Michigan State University

24. Kasper, Jonathan D. The role and regulation of pyruvate dehydrogenase in skeletal muscle bioenergetics and type 2 diabetes.

Degree: 2018, Michigan State University

URL: http://etd.lib.msu.edu/islandora/object/etd:19655

►

Thesis Ph. D. Michigan State University. Physiology 2018

As of 2015 over 30 million people had type 2 diabetes with another 84.1 million with prediabetes… (more)

Subjects/Keywords: Non-insulin-dependent diabetes; Striated muscle – Physiology; Glucose – Physiological transport; Oxidation, Physiological; Pyruvate carboxylase; Physiology

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APA (6^th Edition):

Kasper, J. D. (2018). The role and regulation of pyruvate dehydrogenase in skeletal muscle bioenergetics and type 2 diabetes. (Thesis). Michigan State University. Retrieved from http://etd.lib.msu.edu/islandora/object/etd:19655

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kasper, Jonathan D. “The role and regulation of pyruvate dehydrogenase in skeletal muscle bioenergetics and type 2 diabetes.” 2018. Thesis, Michigan State University. Accessed January 27, 2021. http://etd.lib.msu.edu/islandora/object/etd:19655.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kasper, Jonathan D. “The role and regulation of pyruvate dehydrogenase in skeletal muscle bioenergetics and type 2 diabetes.” 2018. Web. 27 Jan 2021.

Vancouver:

Kasper JD. The role and regulation of pyruvate dehydrogenase in skeletal muscle bioenergetics and type 2 diabetes. [Internet] [Thesis]. Michigan State University; 2018. [cited 2021 Jan 27]. Available from: http://etd.lib.msu.edu/islandora/object/etd:19655.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kasper JD. The role and regulation of pyruvate dehydrogenase in skeletal muscle bioenergetics and type 2 diabetes. [Thesis]. Michigan State University; 2018. Available from: http://etd.lib.msu.edu/islandora/object/etd:19655

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

25. Hakala, Joshua. Investigation of the Mechanisms Governing Carrier Domain Translocation in S. Aureus Pyruvate Carboxylase.

Degree: 2019, Marquette University

URL: https://epublications.marquette.edu/dissertations_mu/894

► A central debate in protein biochemistry focuses on the mechanism by which ligands contribute to conformational changes in proteins. Two primary hypotheses describe this process:… (more)

▼ A central debate in protein biochemistry focuses on the mechanism by which ligands contribute to conformational changes in proteins. Two primary hypotheses describe this process: the induced-fit hypothesis and the conformational ensembles hypothesis. In contrast to the induced-fit hypothesis, the conformational ensembles hypothesis states that the protein pre-exists in multiple conformational states, with ligand binding shifting the equilibrium towards a preferred conformation. The contribution of ligand binding to large-scale conformational changes is particularly relevant to multi-domain “swinging-arm” enzymes, for which pyruvate carboxylase (PC) serves as a well characterized paradigm system. PC catalyzes the ATP-dependent carboxylation of pyruvate to oxaloacetate. The biotin cofactor on the biotin carboxyl carrier protein (BCCP) domain is carboxylated via a MgATP-dependent reaction in the biotin carboxylase (BC) domain. The BCCP domain then travels to the carboxyltransferase (CT) domain where the carboxyl group is transferred from the biotin cofactor to pyruvate, generating oxaloacetate. There have been conflicting proposals for how ligand binding governs carrier domain translocation in PC but, to date, there have been no attempts to directly observe the positioning of the carrier domain independent of catalytic turnover. To directly observe the equilibrium positioning of the BCCP carrier domain, site-specific cross-linking was used to trap the BCCP domain in two separate orientations during translocation. This approach enabled a detailed analysis of how BCCP domain positioning responds to substrates and allosteric effectors. These studies show that carrier domain positioning is governed by conformational selection, with the carrier domain accessing intermolecular positions irrespective of the presence or identity of the ligand(s). Further, interactions between biotin and several conserved active site residues in both the BC and CT domains serve to shift the carrier domain positioning equilibrium. These studies offer the first compelling evidence that carrier domain positional equilibrium in PC is governed by conformational selection and offer new insights into the molecular level interactions contributing to these conformational changes. Advisors/Committee Members: St. Maurice, Martin, Eddinger, Thomas, Manogaran, Anita.

Subjects/Keywords: carrier domain; conformational change; conformational selection; dynamic equilibrium; pyruvate carboxylase; translocation; Biology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hakala, J. (2019). Investigation of the Mechanisms Governing Carrier Domain Translocation in S. Aureus Pyruvate Carboxylase. (Thesis). Marquette University. Retrieved from https://epublications.marquette.edu/dissertations_mu/894

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Hakala, Joshua. “Investigation of the Mechanisms Governing Carrier Domain Translocation in S. Aureus Pyruvate Carboxylase.” 2019. Thesis, Marquette University. Accessed January 27, 2021. https://epublications.marquette.edu/dissertations_mu/894.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Hakala, Joshua. “Investigation of the Mechanisms Governing Carrier Domain Translocation in S. Aureus Pyruvate Carboxylase.” 2019. Web. 27 Jan 2021.

Vancouver:

Hakala J. Investigation of the Mechanisms Governing Carrier Domain Translocation in S. Aureus Pyruvate Carboxylase. [Internet] [Thesis]. Marquette University; 2019. [cited 2021 Jan 27]. Available from: https://epublications.marquette.edu/dissertations_mu/894.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hakala J. Investigation of the Mechanisms Governing Carrier Domain Translocation in S. Aureus Pyruvate Carboxylase. [Thesis]. Marquette University; 2019. Available from: https://epublications.marquette.edu/dissertations_mu/894

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

The Ohio State University

26. Ferreira, Gonzalo. Effect of biotin supplementation on the metabolism of lactating dairy cows.

Degree: PhD, Animal Science, 2006, The Ohio State University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=osu1141386276

► Biotin requirements for lactating dairy cows have not been established. Several studies observed that biotin supplementation increases milk yield by dairy cows, although this response… (more)

▼ Biotin requirements for lactating dairy cows have not been established. Several studies observed that biotin supplementation increases milk yield by dairy cows, although this response was not consistent. The overall objective of this research was to enhance our understanding of when and how biotin supplementation increases milk production by lactating dairy cows. In the first study, we evaluated the urinary excretion of 3-hydroxy-isovaleric acid (3HIA) as a determinant of biotin status. We hypothesized that high-producing cows have a greater need for biotin, and therefore would excrete more 3HIA than low-producing cows. We also hypothesized that biotin supplementation decreases 3HIA excretion. Biotin supplementation increased milk yield in high-producing cows, but not in low-producing cows. Neither production nor biotin supplementation affected the basal urinary excretion of 3HIA. We concluded that urinary excretion of 3HIA is not a sensitive determinant of biotin status in lactating dairy cows. Other measures of biotin status, such as the concentration of avidin-binding substances (ABS) in plasma, milk and urine, were also evaluated. Contrary to our expectations, high-producing and low-producing cows consuming the control diet had similar ABS concentrations in plasma and milk and similar ABS to creatinine ratios in urine. A sensitive indicator of biotin status for lactating dairy cows is still needed. The mechanism by which biotin supplementation increases milk production is not known. In the second study, we hypothesized that biotin supplementation increases the activity and the gene expression of propionyl-CoA carboxylase (PCC) and pyruvate carboxylase (PC) in the liver of high producing dairy cows. Biotin supplementation tended to increase the activity of PC, but did not affect the activity of PCC. The differential response to biotin supplementation suggests that PCC may have a higher priority for biotin than PC. Biotin supplementation did not affect the gene expression of PCC and PC. We concluded that biotin supplementation can affect the activity of biotin-dependent carboxylases in the liver of lactating dairy cows. Whether biotin supplementation increases glucose production in the liver still needs to be evaluated. Advisors/Committee Members: Weiss, William (Advisor).

Subjects/Keywords: Biotin; Dairy cows; 3-hydroxyisovaleric acid; Pyruvate carboxylase; Propionyl-CoA carboxylase

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ferreira, G. (2006). Effect of biotin supplementation on the metabolism of lactating dairy cows. (Doctoral Dissertation). The Ohio State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=osu1141386276

Chicago Manual of Style (16^th Edition):

Ferreira, Gonzalo. “Effect of biotin supplementation on the metabolism of lactating dairy cows.” 2006. Doctoral Dissertation, The Ohio State University. Accessed January 27, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=osu1141386276.

MLA Handbook (7^th Edition):

Ferreira, Gonzalo. “Effect of biotin supplementation on the metabolism of lactating dairy cows.” 2006. Web. 27 Jan 2021.

Vancouver:

Ferreira G. Effect of biotin supplementation on the metabolism of lactating dairy cows. [Internet] [Doctoral dissertation]. The Ohio State University; 2006. [cited 2021 Jan 27]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1141386276.

Council of Science Editors:

Ferreira G. Effect of biotin supplementation on the metabolism of lactating dairy cows. [Doctoral Dissertation]. The Ohio State University; 2006. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1141386276

Universidade Estadual de Campinas

27. Menezes, Eveline Soares, 1992-. Busca por alvo molecular associado ao processo de biogênese mitocondrial em células pancreáticas secretoras de insulina: Molecular target search associated to the process of mitochondrial biogenesis in insulin secreting pancreatic cells.

Degree: 2018, Universidade Estadual de Campinas

URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/331818

► Abstract: The development of Type 2 Diabetes (T2D) occurs due to the pancreatic beta cells dysfunction. The literature shows a consensus that in the early… (more)

▼ Abstract: The development of Type 2 Diabetes (T2D) occurs due to the pancreatic beta cells dysfunction. The literature shows a consensus that in the early stages of this disease exists a gain in the mass content of pancreatic beta cells, followed by increased production and secretion of insulin. While in the chronic stage, there is a reduction of the mass, followed by lower insulin production, suggesting a close association between cell mass content and mitochondrial oxidative capacity. During T2D there is also a failure of pancreatic beta cells adaptation. Pyruvate Carboxylase (PC) is an important enzyme in pancreatic beta-cells and plays essential roles in the insulin secretion; is the major product of glycolysis in these cells and is responsible for beta cell adaptation and for anaplerosis, which is fundamental to control the Krebs cycle. However, molecular control of mitochondrial biogenesis in beta cell is still unclear. Although the literature demonstrates an increasing of the PC levels during the insulin secretion, and consequently, a higher mitochondrial activity too, little is known about the regulation of mitochondrial biogenesis process of these cells. We have shown that some of the proteins interacting with the distal promoter of PC, which is responsible for anaplerosis, might be possible targets related to the control of the mitochondrial function and biogenesis, hence, to the insulin secretion in MIN6 pancreatic cells. Therefore, the study of molecular mechanisms regarding to beta cells protection against impaired insulin secretion and mitochondrial function will be relevant to elucidate new molecules able to enhance both function and survival of insulin secreting cells Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS (CRUESP), Silveira, Leonardo dos Reis, 1970- (advisor), Universidade Estadual de Campinas. Instituto de Biologia (institution), Programa de Pós-Graduação em Biologia Funcional e Molecular (nameofprogram), Sampaio, Helena Cristina de Lima Barbosa (committee member), Persinoti, Gabriela Felix (committee member).

Subjects/Keywords: Piruvato carboxilase; Células secretoras de insulina; Mitocôndria - Fisiologia; Insulina - Secreção; Pyruvate carboxylase; Insulin - Secreting cells; Mitochondria - Physiology; Insulin - Secretion

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APA (6^th Edition):

Menezes, Eveline Soares, 1. (2018). Busca por alvo molecular associado ao processo de biogênese mitocondrial em células pancreáticas secretoras de insulina: Molecular target search associated to the process of mitochondrial biogenesis in insulin secreting pancreatic cells. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/331818

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Menezes, Eveline Soares, 1992-. “Busca por alvo molecular associado ao processo de biogênese mitocondrial em células pancreáticas secretoras de insulina: Molecular target search associated to the process of mitochondrial biogenesis in insulin secreting pancreatic cells.” 2018. Thesis, Universidade Estadual de Campinas. Accessed January 27, 2021. http://repositorio.unicamp.br/jspui/handle/REPOSIP/331818.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Menezes, Eveline Soares, 1992-. “Busca por alvo molecular associado ao processo de biogênese mitocondrial em células pancreáticas secretoras de insulina: Molecular target search associated to the process of mitochondrial biogenesis in insulin secreting pancreatic cells.” 2018. Web. 27 Jan 2021.

Vancouver:

Menezes, Eveline Soares 1. Busca por alvo molecular associado ao processo de biogênese mitocondrial em células pancreáticas secretoras de insulina: Molecular target search associated to the process of mitochondrial biogenesis in insulin secreting pancreatic cells. [Internet] [Thesis]. Universidade Estadual de Campinas; 2018. [cited 2021 Jan 27]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/331818.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Menezes, Eveline Soares 1. Busca por alvo molecular associado ao processo de biogênese mitocondrial em células pancreáticas secretoras de insulina: Molecular target search associated to the process of mitochondrial biogenesis in insulin secreting pancreatic cells. [Thesis]. Universidade Estadual de Campinas; 2018. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/331818

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

28. Wyatt, Brittney. The Discovery and Characterization of Small Molecule Inhibitors of Pyruvate Carboxylase.

Degree: 2018, Marquette University

URL: https://epublications.marquette.edu/dissertations_mu/1008

► Pyruvate carboxylase is a metabolically important enzyme that has been implicated in a wide range of diseases and infections such as cancer, diabetes and listeriosis.… (more)

Subjects/Keywords: Fast-Violet B; High-throughput screening; Phenylpyruvic acid scaffold; pyruvate carboxylase; small molecule inhibitors; Structure-based drug design; Biology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wyatt, B. (2018). The Discovery and Characterization of Small Molecule Inhibitors of Pyruvate Carboxylase. (Thesis). Marquette University. Retrieved from https://epublications.marquette.edu/dissertations_mu/1008

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Wyatt, Brittney. “The Discovery and Characterization of Small Molecule Inhibitors of Pyruvate Carboxylase.” 2018. Thesis, Marquette University. Accessed January 27, 2021. https://epublications.marquette.edu/dissertations_mu/1008.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Wyatt, Brittney. “The Discovery and Characterization of Small Molecule Inhibitors of Pyruvate Carboxylase.” 2018. Web. 27 Jan 2021.

Vancouver:

Wyatt B. The Discovery and Characterization of Small Molecule Inhibitors of Pyruvate Carboxylase. [Internet] [Thesis]. Marquette University; 2018. [cited 2021 Jan 27]. Available from: https://epublications.marquette.edu/dissertations_mu/1008.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Wyatt B. The Discovery and Characterization of Small Molecule Inhibitors of Pyruvate Carboxylase. [Thesis]. Marquette University; 2018. Available from: https://epublications.marquette.edu/dissertations_mu/1008

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

29. Habarou, Florence. Métabolisme de l'acétyl-CoA : modulation pharmacologique, approches thérapeutiques et nouvelles maladies : Acetyl-coA metabolism : pharmacological treatment, therapeutic approaches and new diseases.

Degree: Docteur es, Biologie cellulaire, 2016, Sorbonne Paris Cité

URL: http://www.theses.fr/2016USPCB104

► L’acétyl-coA occupe une place centrale dans le métabolisme intermédiaire. Il constitue le point de jonction de plusieurs voies métaboliques telles que la .-oxydation, la glycolyse,… (more)

▼ L’acétyl-coA occupe une place centrale dans le métabolisme intermédiaire. Il constitue le point de jonction de plusieurs voies métaboliques telles que la .-oxydation, la glycolyse, le catabolisme de certains acides aminés, la cétolyse, la cétogenèse et la synthèse d’acides gras. Il est également impliqué dans d’autres processus tels que l’acétylation des protéines. Au cours de mon travail de thèse, je me suis attachée à étudier différents aspects du métabolisme de l’acétyl-coA. La première partie de mon travail a porté sur la modulation pharmacologique de la .- oxydation dans le but de corriger des déficits de cette voie métabolique. L’intérêt de traitements par 400µM de bézafibrate ou 75µM de resvératrol dans les formes modérées de déficit en VLCAD et en CPT2 avait été montré précédemment. Par des méthodes de référence et grâce à la mise au point de nouvelles techniques, j’ai pu montrer sur des fibroblastes de patients déficitaires en LCHAD que des traitements par une combinaison de 35µM de bézafibrate et 30µM de resvératrol permettent d’augmenter les capacités d’oxydation du palmitate en stimulant la synthèse protéique. L’effet de cette combinaison était comparable à celui d’un traitement par 400µM de bézafibrate. Dans un second temps, je me suis intéressée à deux cofacteurs impliqués dans le métabolisme de l’acétyl-coA : l’acide lipoïque, cofacteur de quatre .-cétoacides déshydrogénases (PDHc, BCKDHc, .- KGDHc et GCS) et la riboflavine, cofacteur d’acyl-coA déshydrogénases de la .-oxydation et de déshydrogénases impliquées dans le catabolisme des acides aminés ramifiés. Ainsi, j’ai participé à la description d’anomalies du métabolisme de l’acide lipoïque, un nouveau groupe de maladies héréditaires du métabolisme caractérisé par un déficit combiné en .-cétoacides déshydrogénases. Par ailleurs, j’ai pu montrer qu’une hyperprolinémie constitue un biomarqueur intéressant pour le diagnostic d’acidurie glutarique de type II primaire ou secondaire, ces dernières pouvant se rencontrer en cas d’anomalie du métabolisme de la riboflavine. J’ai également évalué l’utilisation d’un mélange racémique de L,D-3-hydroxybutyrate afin de corriger les déficits énergétiques induits par un déficit en PDHc ou GLUT1. Via la cétolyse, le L,D-3- hydroxybutyrate génère de l’acétyl-coA. De façon surprenante, l’administration de ce composé s’est traduite par une amélioration de l’état clinique des patients atteints de déficits en PDHc, alors qu’une dégradation a été observée chez les patients atteints de déficits en GLUT1. Cette évolution différente pourrait souligner l’importance de l’anaplérose chez les patients déficitaires en GLUT1. Enfin, la dernière partie de mon travail de thèse porte sur la description d’un patient atteint d’une forme modérée de déficit en pyruvate carboxylase, cette enzyme étant régulée par l’acétyl-coA. Les difficultés diagnostiques rencontrées devant ces formes modérées sont rapportées, ainsi que des essais de traitement par des composés anaplérotiques et par le bézafibrate, malheureusement sans bénéfice net que ce… Advisors/Committee Members: Bastin, Jean (thesis director).

Subjects/Keywords: Acétyl-CoA; Maladies héréditaires du métabolisme; Beta-oxydation des acides gras; Bézafibrate; Resvératrol; Acide lipoïque; PDHc; GLUT1; Corps cétoniques; Pyruvate carboxylase; Analyse de flux métaboliques; Acetyl-coA metabolism; Inherited metabolic diseases; Fatty acid beta-oxidation; Bezafibrate; Resveratrol; Lipoic acid; PDH; GLUT1; Ketone bodies; Pyruvate carboxylase; Metabolic flux analysis; 572.4

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Habarou, F. (2016). Métabolisme de l'acétyl-CoA : modulation pharmacologique, approches thérapeutiques et nouvelles maladies : Acetyl-coA metabolism : pharmacological treatment, therapeutic approaches and new diseases. (Doctoral Dissertation). Sorbonne Paris Cité. Retrieved from http://www.theses.fr/2016USPCB104

Chicago Manual of Style (16^th Edition):

Habarou, Florence. “Métabolisme de l'acétyl-CoA : modulation pharmacologique, approches thérapeutiques et nouvelles maladies : Acetyl-coA metabolism : pharmacological treatment, therapeutic approaches and new diseases.” 2016. Doctoral Dissertation, Sorbonne Paris Cité. Accessed January 27, 2021. http://www.theses.fr/2016USPCB104.

MLA Handbook (7^th Edition):

Habarou, Florence. “Métabolisme de l'acétyl-CoA : modulation pharmacologique, approches thérapeutiques et nouvelles maladies : Acetyl-coA metabolism : pharmacological treatment, therapeutic approaches and new diseases.” 2016. Web. 27 Jan 2021.

Vancouver:

Habarou F. Métabolisme de l'acétyl-CoA : modulation pharmacologique, approches thérapeutiques et nouvelles maladies : Acetyl-coA metabolism : pharmacological treatment, therapeutic approaches and new diseases. [Internet] [Doctoral dissertation]. Sorbonne Paris Cité; 2016. [cited 2021 Jan 27]. Available from: http://www.theses.fr/2016USPCB104.

Council of Science Editors:

Habarou F. Métabolisme de l'acétyl-CoA : modulation pharmacologique, approches thérapeutiques et nouvelles maladies : Acetyl-coA metabolism : pharmacological treatment, therapeutic approaches and new diseases. [Doctoral Dissertation]. Sorbonne Paris Cité; 2016. Available from: http://www.theses.fr/2016USPCB104

East Carolina University

30. Westerhold, Lauren. Kinetic and Thermodynamic Contributions to an Intermolecular Mechanism of Subunit Communication: Coordination of Pyruvate Carboxylase Activity Among Spatially Distinct Active Sites.

Degree: MS, MS-Biomedical Science, 2016, East Carolina University

URL: http://hdl.handle.net/10342/5890

► Catalysis occurring in a multifunctional enzyme at spatially distinct active sites is controlled by an array of factors, including the structure of the enzyme, ligand… (more)

▼ Catalysis occurring in a multifunctional enzyme at spatially distinct active sites is controlled by an array of factors, including the structure of the enzyme, ligand binding, and productive interaction of substrates to facilitate turnover. Successful execution of the catalytic cycle is partially dependent upon the ability of spatially and functionally discrete active sites to communicate with one another, as well as with any allosteric regulatory regions of the enzyme. This type of long-range communication typically manifests measurable effects on substrate binding or product release. In the case of pyruvate carboxylase (PC), pyruvate binding to the carboxyl transferase (CT) domain induces translocation of the biotin carboxyl carrier (BCCP) domain and subsequently increases the rate of Pi release in the biotin carboxylase (BC) domain. While the kinetic mechanism and structural arrangement of the PC tetramer has largely been elucidated, the source of the intermolecular signals required to facilitate catalysis between distinct active sites remains unclear. The BC and CT domain active sites necessary to produce one oxaloacetate are located on two separate polypeptide chains, while binding of acetyl-CoA in its pocket formed between the allosteric domain and the BC domain is required for stimulation of the overall catalytic rate. In metabolic regulatory enzymes such as PC, it is essential to understand not only the overall mechanism of intersubunit communication, but also the thermodynamic driving forces behind each individual ligand relationship in order to piece together the network of amino acids and subunit domains that is responsible for the dramatic stimulatory response elicited upon binding of acetyl-CoA, the enzyme's essential allosteric activator. Ultimately, this would allow for elucidation of the molecular regulatory mechanism of PC and for subsequent development of therapeutic strategies to target the chronic hyperglycemia associated with its uncontrolled activity in Type 2 diabetics. To address how pyruvate occupancy in the CT domain impacts the behavior of other domains, we generated mixed hybrid tetramers using mutants of the catalytically relevant residues Glu218 (in the BC domain) and Thr882 (in the CT domain) and measured both the pyruvate carboxylation and inorganic phosphate release activities. Our results, which compared the apparent Ka pyruvate for pyruvate-stimulated Pi release catalyzed by the T882S:E218A(1:1) hybrid tetramer to that of the wild-type and the T882S homotetramer, were consistent with an intermolecular mechanism of subunit communication, whereby pyruvate binding at the T882S CT domain was responsible for inducing translocation of the E218A BCCP domain within the same face of the tetramer. We also determined the thermodynamic-linkage of each ligand of PC, that is, the extent to which the presence of one bound substrate or effector positively or negatively influences enzyme turnover in the presence of saturating and subsaturating concentrations of another. The ability of either MgATP… Advisors/Committee Members: Zeczycki, Tonya (advisor).

Subjects/Keywords: carboxyltransferase; CT; biotin carboxylase; BC; biotin carboxyl carrier; BCCP; kinetics; thermodynamics; diabetes; metabolism; intermolecular; mechanism; communication; active site; allosterism; allosteric regulation; acetyl CoA; thermodynamic linkage; tetramer; monomer; hybrid tetramer; Ligases; Musculoskeletal Physiological Phenomena; Catalytic Domain; Kinetics; Thermodynamics; Pyruvate Carboxylase

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Westerhold, L. (2016). Kinetic and Thermodynamic Contributions to an Intermolecular Mechanism of Subunit Communication: Coordination of Pyruvate Carboxylase Activity Among Spatially Distinct Active Sites. (Masters Thesis). East Carolina University. Retrieved from http://hdl.handle.net/10342/5890

Chicago Manual of Style (16^th Edition):

Westerhold, Lauren. “Kinetic and Thermodynamic Contributions to an Intermolecular Mechanism of Subunit Communication: Coordination of Pyruvate Carboxylase Activity Among Spatially Distinct Active Sites.” 2016. Masters Thesis, East Carolina University. Accessed January 27, 2021. http://hdl.handle.net/10342/5890.

MLA Handbook (7^th Edition):

Westerhold, Lauren. “Kinetic and Thermodynamic Contributions to an Intermolecular Mechanism of Subunit Communication: Coordination of Pyruvate Carboxylase Activity Among Spatially Distinct Active Sites.” 2016. Web. 27 Jan 2021.

Vancouver:

Westerhold L. Kinetic and Thermodynamic Contributions to an Intermolecular Mechanism of Subunit Communication: Coordination of Pyruvate Carboxylase Activity Among Spatially Distinct Active Sites. [Internet] [Masters thesis]. East Carolina University; 2016. [cited 2021 Jan 27]. Available from: http://hdl.handle.net/10342/5890.

Council of Science Editors:

Westerhold L. Kinetic and Thermodynamic Contributions to an Intermolecular Mechanism of Subunit Communication: Coordination of Pyruvate Carboxylase Activity Among Spatially Distinct Active Sites. [Masters Thesis]. East Carolina University; 2016. Available from: http://hdl.handle.net/10342/5890

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Open Access Theses and Dissertations