subject:(Proteins Synthesis)

Hong Kong University of Science and Technology

1. Liu, Wen. Reexamination of G protein activation model by non-dissociable Gα-Gβ fusion proteins.

Degree: 2015, Hong Kong University of Science and Technology

URL: http://repository.ust.hk/ir/Record/1783.1-86335 ; https://doi.org/10.14711/thesis-b1514991 ; http://repository.ust.hk/ir/bitstream/1783.1-86335/1/th_redirect.html

► Activation of heterotrimeric G proteins is commonly believed to trigger the dissociation of Gβγ dimers from GTP-bound Gα subunits. Such a model is mainly based… (more)

Subjects/Keywords: G proteins ; Proteins ; Synthesis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Liu, W. (2015). Reexamination of G protein activation model by non-dissociable Gα-Gβ fusion proteins. (Thesis). Hong Kong University of Science and Technology. Retrieved from http://repository.ust.hk/ir/Record/1783.1-86335 ; https://doi.org/10.14711/thesis-b1514991 ; http://repository.ust.hk/ir/bitstream/1783.1-86335/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Liu, Wen. “Reexamination of G protein activation model by non-dissociable Gα-Gβ fusion proteins.” 2015. Thesis, Hong Kong University of Science and Technology. Accessed January 22, 2021. http://repository.ust.hk/ir/Record/1783.1-86335 ; https://doi.org/10.14711/thesis-b1514991 ; http://repository.ust.hk/ir/bitstream/1783.1-86335/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Liu, Wen. “Reexamination of G protein activation model by non-dissociable Gα-Gβ fusion proteins.” 2015. Web. 22 Jan 2021.

Vancouver:

Liu W. Reexamination of G protein activation model by non-dissociable Gα-Gβ fusion proteins. [Internet] [Thesis]. Hong Kong University of Science and Technology; 2015. [cited 2021 Jan 22]. Available from: http://repository.ust.hk/ir/Record/1783.1-86335 ; https://doi.org/10.14711/thesis-b1514991 ; http://repository.ust.hk/ir/bitstream/1783.1-86335/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Liu W. Reexamination of G protein activation model by non-dissociable Gα-Gβ fusion proteins. [Thesis]. Hong Kong University of Science and Technology; 2015. Available from: http://repository.ust.hk/ir/Record/1783.1-86335 ; https://doi.org/10.14711/thesis-b1514991 ; http://repository.ust.hk/ir/bitstream/1783.1-86335/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Columbia University

2. Huang, Bridget Yih Jiin. Regulation of Release Factor 2 in Non-canonical Translation Pathways.

Degree: 2017, Columbia University

URL: https://doi.org/10.7916/D80P10MC

► Protein synthesis, or translation, is a complex, multi-step process that requires regulatory and quality control mechanisms to ensure the accurate production of proteins. Two major… (more)

▼ Protein synthesis, or translation, is a complex, multi-step process that requires regulatory and quality control mechanisms to ensure the accurate production of proteins. Two major challenges during bacterial protein synthesis are maintaining the accuracy of translation during the elongation stage and resolving stalled ribosomal complexes. Interestingly, bacteria have evolved two mechanisms, a post-peptidyl transfer quality control (post PT QC) and a ribosome rescue mechanism, to counter these challenges. Both of these mechanisms make use of a protein factor that normally functions during translation termination, Release Factor 2 (RF2), along with an additional protein factor, Release Factor 3 (RF3) for post PT QC and Alternative ribosome-rescue factor A (ArfA) for ribosome rescue, to achieve these non-canonical functions. The mechanistic role of RF3 and ArfA in these two pathways remains unclear; however, they may play a role in regulating RF2 in context of these non-canonical pathways. As a step toward understanding the role of RF3 and ArfA in post PT QC and ribosome rescue and, in particular, their role in the regulation of RF2, I sought to determine the effect of RF3 and ArfA on the binding kinetics of RF2 in post PT QC and ribosome rescue pathways. Using a single-molecule fluorescence resonance energy transfer (smFRET) signal between the P-site peptidyl-tRNA and RF2, the binding and dissociation of RF2 can be directly monitored in the absence or in the presence of RF3 or ArfA. In Chapter 2, I describe the development of smFRET signals using different chromophores, cyanine 3 to cyanine 5 (Cy3-Cy5) or to a fluorescence quencher (Cy3-QSY9). The Cy3-Cy5 and Cy3-QSY9 smFRET signals complement each other for monitoring RF2 binding; whereas Cy3-Cy5 is suitable for observing stable binding using low substrate concentration, Cy3-QSY9 is suitable for observing transient binding using high substrate concentration. The RF2 binding and dissociation to ribosomal complexes was first examined in the absence of other factors thus providing the foundation for studying the regulation of RF2 binding by RF3 or ArfA. In the bacterial post PT QC mechanism, RF3 enhances the rate of RF2-mediated peptide release to catalyze premature termination of miscoded protein, thus ultimately increasing the fidelity of protein synthesis1. Without addition of RF3, the rate of RF2-mediated peptide release is too slow to compete with the rate of protein synthesis. In Chapter 3, the role of RF3 on RF2 binding kinetics in post PT QC was investigated using both an fMet-Lys-tRNALys(Cy3) to RF2(Cy5) smFRET signal and an fMet-Lys-tRNALys(Cy3) to RF2(QSY9) smFRET signal. The ArfA-RF2 ribosome rescue pathway is a backup mechanism for trans-translation, which relieves stalled ribosomal complexes by providing an open reading frame coding for both a degradation tag and a stop codon2. Because the expression of ArfA is under strict control by trans-translation, the ArfA-RF2 pathway only functions in the absence of active trans-translation. More…

Subjects/Keywords: Bacterial proteins; Ribosomes; Biochemistry; Proteins – Synthesis; Biophysics

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Huang, B. Y. J. (2017). Regulation of Release Factor 2 in Non-canonical Translation Pathways. (Doctoral Dissertation). Columbia University. Retrieved from https://doi.org/10.7916/D80P10MC

Chicago Manual of Style (16^th Edition):

Huang, Bridget Yih Jiin. “Regulation of Release Factor 2 in Non-canonical Translation Pathways.” 2017. Doctoral Dissertation, Columbia University. Accessed January 22, 2021. https://doi.org/10.7916/D80P10MC.

MLA Handbook (7^th Edition):

Huang, Bridget Yih Jiin. “Regulation of Release Factor 2 in Non-canonical Translation Pathways.” 2017. Web. 22 Jan 2021.

Vancouver:

Huang BYJ. Regulation of Release Factor 2 in Non-canonical Translation Pathways. [Internet] [Doctoral dissertation]. Columbia University; 2017. [cited 2021 Jan 22]. Available from: https://doi.org/10.7916/D80P10MC.

Council of Science Editors:

Huang BYJ. Regulation of Release Factor 2 in Non-canonical Translation Pathways. [Doctoral Dissertation]. Columbia University; 2017. Available from: https://doi.org/10.7916/D80P10MC

Oregon State University

3. Allen, Renee Allana. In vitro estradiol-induced protein synthesis in the endometrium of cyclic and steroid-treated ovariectomized ewes.

Degree: MS, Animal Science, 1977, Oregon State University

URL: http://hdl.handle.net/1957/43562

► The ability of ovine endometrium incubated in vitro with estradiol -17(3 to synthesize a specific induced protein (IP) was studied. Endometrium from cycling, pregnant and… (more)

▼ The ability of ovine endometrium incubated in vitro with estradiol -17(3 to synthesize a specific induced protein (IP) was studied. Endometrium from cycling, pregnant and ovariectomized ewes was used. Twelve ewes were allotted in equal numbers to be sacrificed on days 0 (estrus), 3, 6, or 10 of the estrous cycle. In addition uteri from nonpregnant ewes (day 14 or 15, N = 3) were compared with day 15 pregnant ewes (N = 3). Experiment 2 involved 16 ovariectomized ewes assigned randomly to one of four treatment regimes. The ewes were treated as follows: a) controls, no exogenous steroids, b) estradiol -17β, c) progesterone and d) progesterone plus estradiol. Estradiol was packed into silastic capsules and implanted subcutaneously on day 1 of treatment. Progesterone (15 mg 2x daily) or vehicle (oil) were injected for 5 consecutive days beginning 48 hr (day 3) after implantation of estradiol. All ewes were sacrificed on the morning of day 8. Blood samples were taken at sacrifice and estradiol and progesterone levels were measured. The ewes treated with estradiol tended to have increased estradiol levels and ewes receiving progesterone had significantly higher serum progesterone levels. Upon sacrifice of the ewes, the uterus was removed and transported to the laboratory. Three-50 mg endometrial segments were incubated for 1 hr with either 2.5 x 10⁻⁸ M estradio1-17β (treated) or ethanol (control), followed by a 2 or 4 hr incubation with labelled amino acids. Treated and control tissues were homogenized together and the resulting supernatant was stored at -20 C until electro phoresed. Following disc gel electrophoresis, the gels were placed into a dye for staining or were fixed in acetic acid. The gels fixed with acetic acid were sliced into sections and then counted in a scintillation spectrometer. Experiments utilizing immature rat uteri were conducted to verify that IP synthesis in the rat uterus could be detected in our laboratory. Induced protein was detected following estradiol stimulation in vivo and in vitro. Synthesis of an estradiol-induced endometrial protein was not detected during the ovine estrous cycle, on day 15 of pregnancy or in steroid-treated ovariectomized ewes. However, increased incorporation of both isotopes, running in front of the tracker dye, was detected in two of the four ewes in both the control and estradioltreated ovariectomized ewes. This increased isotope incorporation was detected in all four ewes receiving progesterone injections and was not present in any of the ewes receiving both estradiol and progesterone. Problems with excess estradiol during the in vitro incubation probably prevented any expression of IP during the estrous cycle experiment (experiment 1). No substantial change in the prealbumin, non-specific protein patterns were observed. Protein profiles of uteri varied from 1 to 3 bands during the estrous cycle and day 15 of pregnancy. The number of uterine prealbumin bands observed in the ovariectomized ewes receiving exogenous… Advisors/Committee Members: Swanson, Lloyd (advisor).

Subjects/Keywords: Proteins – Synthesis

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APA (6^th Edition):

Allen, R. A. (1977). In vitro estradiol-induced protein synthesis in the endometrium of cyclic and steroid-treated ovariectomized ewes. (Masters Thesis). Oregon State University. Retrieved from http://hdl.handle.net/1957/43562

Chicago Manual of Style (16^th Edition):

Allen, Renee Allana. “In vitro estradiol-induced protein synthesis in the endometrium of cyclic and steroid-treated ovariectomized ewes.” 1977. Masters Thesis, Oregon State University. Accessed January 22, 2021. http://hdl.handle.net/1957/43562.

MLA Handbook (7^th Edition):

Allen, Renee Allana. “In vitro estradiol-induced protein synthesis in the endometrium of cyclic and steroid-treated ovariectomized ewes.” 1977. Web. 22 Jan 2021.

Vancouver:

Allen RA. In vitro estradiol-induced protein synthesis in the endometrium of cyclic and steroid-treated ovariectomized ewes. [Internet] [Masters thesis]. Oregon State University; 1977. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/1957/43562.

Council of Science Editors:

Allen RA. In vitro estradiol-induced protein synthesis in the endometrium of cyclic and steroid-treated ovariectomized ewes. [Masters Thesis]. Oregon State University; 1977. Available from: http://hdl.handle.net/1957/43562

Oregon State University

4. Abdul-Latif, Fazilah. Modulation of translational fidelity by small subunit ribosoma RNA from Escherichia coli.

Degree: PhD, Biochemistry and Biophysics, 1988, Oregon State University

URL: http://hdl.handle.net/1957/40516

► Ribosomes are ribonucleoprotein particles that are essential for the process of protein synthesis in all living systems. Escherichia coli, eubacterial organisms, have ribosomes which display… (more)

Subjects/Keywords: Proteins – Synthesis

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APA (6^th Edition):

Abdul-Latif, F. (1988). Modulation of translational fidelity by small subunit ribosoma RNA from Escherichia coli. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/40516

Chicago Manual of Style (16^th Edition):

Abdul-Latif, Fazilah. “Modulation of translational fidelity by small subunit ribosoma RNA from Escherichia coli.” 1988. Doctoral Dissertation, Oregon State University. Accessed January 22, 2021. http://hdl.handle.net/1957/40516.

MLA Handbook (7^th Edition):

Abdul-Latif, Fazilah. “Modulation of translational fidelity by small subunit ribosoma RNA from Escherichia coli.” 1988. Web. 22 Jan 2021.

Vancouver:

Abdul-Latif F. Modulation of translational fidelity by small subunit ribosoma RNA from Escherichia coli. [Internet] [Doctoral dissertation]. Oregon State University; 1988. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/1957/40516.

Council of Science Editors:

Abdul-Latif F. Modulation of translational fidelity by small subunit ribosoma RNA from Escherichia coli. [Doctoral Dissertation]. Oregon State University; 1988. Available from: http://hdl.handle.net/1957/40516

Hong Kong University of Science and Technology

5. Feng, Yitao. Macromolecular crowding effect on function, stability and dynamics of selected protein systems.

Degree: 2012, Hong Kong University of Science and Technology

URL: http://repository.ust.hk/ir/Record/1783.1-91980 ; https://doi.org/10.14711/thesis-b1207874 ; http://repository.ust.hk/ir/bitstream/1783.1-91980/1/th_redirect.html

► In the cellular environment, biomacromolecules together occupy about 20-30% of the total volume and sometimes up to 40% by estimation. The occupied volume is not… (more)

▼ In the cellular environment, biomacromolecules together occupy about 20-30% of the total volume and sometimes up to 40% by estimation. The occupied volume is not accessible for other molecular activities, creating a crowded condition. However, common biochemistry studies are carried out in test-tubes where the buffer solution is diluted. Although these in vitro studies give us a lot of useful information on properties of biomolecules and help us understand their cellular functions, the difference between the molecular behavior in dilute solutions and in crowding solutions are not well defined. In some cases, the difference may be so significant that researchers might be misled. In order to better understand the behaviors of biomolecules in cells, more and more researchers have developed an interest in the effects of the crowded intracellular environment. In this thesis, we studied crowding effect on selected proteins’ function, stability and fast dynamics. Firstly, we studied the crowding effect on CalE8, the polyketide synthase (PKS) in the biosynthesis pathway of natural products calicheamicin. Previous studies in our research group have already found that a crowding agent can suppress the formation of side-products formed by a two-module nonribosomal peptide synthetase (NRPS) in the biosynthesis pathway of enterobactin. Polyketide synthases are a group of enzymes very similar with nonribosomal peptide synthetases. Here we chose this small PKS CalE8 as a model enzyme and the results showed that crowding agents would not improve the activity and specificity of CalE8. This further demonstrates the complexity of crowding effect on enzyme activity. Subsequently, we studied the crowding on protein stability where calmodulin was used as a model. Previously researchers have already found that the crowding agent Ficoll 70 can improve the thermal stability of calmodulin. In our study, the stabilization effect of Ficoll 70 on calmodulin thermal stability was also observed. Further experiment showed that Ficoll 70 could also stabilize protein chemical stability. Meanwhile, sucrose, the monomer of Ficoll 70 which is structurally similar with Ficoll 70, could improve calmodulin thermal and chemical stability to a similar extent. Considering that sucrose is a small molecule without crowding effect, these results strongly indicate that some previously reported crowding effect is mainly from the solvent effect introduced by high concentration of crowding agent. Finally, we studied the crowding effect on dynamics of calmodulin, which is saturated with calcium ions. Steady state fluorescence was used to monitor the signal of two tyrosine residues at C-terminal domain of calmodulin. At elevated concentration of Ficoll 70, the emission at about 307 nm continuously decreased. In 30% Ficoll 70 solution, the tyrosine signal decreased to as much as about 40%. In comparison, sucrose could not affect calmodulin fluorescence. This Ficoll 70 induced signal decrease was very similar to the fluorescence change at elevated temperature which…

Subjects/Keywords: Macromolecules ; Synthesis ; Polyketides ; Proteins ; Biosynthesis

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APA (6^th Edition):

Feng, Y. (2012). Macromolecular crowding effect on function, stability and dynamics of selected protein systems. (Thesis). Hong Kong University of Science and Technology. Retrieved from http://repository.ust.hk/ir/Record/1783.1-91980 ; https://doi.org/10.14711/thesis-b1207874 ; http://repository.ust.hk/ir/bitstream/1783.1-91980/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Feng, Yitao. “Macromolecular crowding effect on function, stability and dynamics of selected protein systems.” 2012. Thesis, Hong Kong University of Science and Technology. Accessed January 22, 2021. http://repository.ust.hk/ir/Record/1783.1-91980 ; https://doi.org/10.14711/thesis-b1207874 ; http://repository.ust.hk/ir/bitstream/1783.1-91980/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Feng, Yitao. “Macromolecular crowding effect on function, stability and dynamics of selected protein systems.” 2012. Web. 22 Jan 2021.

Vancouver:

Feng Y. Macromolecular crowding effect on function, stability and dynamics of selected protein systems. [Internet] [Thesis]. Hong Kong University of Science and Technology; 2012. [cited 2021 Jan 22]. Available from: http://repository.ust.hk/ir/Record/1783.1-91980 ; https://doi.org/10.14711/thesis-b1207874 ; http://repository.ust.hk/ir/bitstream/1783.1-91980/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Feng Y. Macromolecular crowding effect on function, stability and dynamics of selected protein systems. [Thesis]. Hong Kong University of Science and Technology; 2012. Available from: http://repository.ust.hk/ir/Record/1783.1-91980 ; https://doi.org/10.14711/thesis-b1207874 ; http://repository.ust.hk/ir/bitstream/1783.1-91980/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Missouri – Columbia

6. Shebl, Bassem. The influence of mRNA secondary structures on ribosome conformational dynamics.

Degree: 2016, University of Missouri – Columbia

URL: http://hdl.handle.net/10355/62520

► [ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT AUTHOR'S REQUEST.] Synthesizing proteins in the cell is a critical aspect of life. Protein synthesis is a… (more)

▼ [ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT AUTHOR'S REQUEST.] Synthesizing proteins in the cell is a critical aspect of life. Protein synthesis is a complicated process and involve highly functional machines at a molecular level. The ribosome is the molecular machine that translate coded sequences of nucleic acids into functional proteins. Understanding how ribosomes function is key to understanding protein synthesis / translation. We focus our work on ribosomes from bacterial cells. This allows us to study much simpler systems and extrapolate our knowledge to higher levels. One key challenge in the field is to be able to isolate a high quantity of good and active ribosomes out of the cell to study it in a controlled environment. Classically known methods involve extensive resources, high technical expertise, and a week of preparation. We developed a one-step protocol to purify ribosomes that are more active than the ones purified from classical methods. This developed technique saves time and money and results in much higher amounts of product. This approach also makes the technique approachable to a wider community of scientists and researchers. The same methodology could be applied towards purifying other molecular machines in the cells. Using these ribosomes, we wanted to investigate how the ribosomes function in cells when faced with specific signals. These signals are utilized by the cells to control protein synthesis. However, in dome diseased cells and for some viruses, normal protein synthesis is overridden by the invaders to produce faulty proteins that could result in a wide range of diseases such as Alzheimer and others. In this study, we investigated how the ribosome functions in the presence of such signals and how close do they need to be to the ribosome to affect protein synthesis. This allows us to design drugs to mimic or inhibit such changes thus fixing faulty protein production or sometimes induce it to inhibit protein synthesis in bacterial cells and as such designing and producing novel drugs. Advisors/Committee Members: Cornish, Peter V. (advisor).

Subjects/Keywords: Proteins – Synthesis; Messenger RNA; Ribosomes

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Shebl, B. (2016). The influence of mRNA secondary structures on ribosome conformational dynamics. (Thesis). University of Missouri – Columbia. Retrieved from http://hdl.handle.net/10355/62520

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Shebl, Bassem. “The influence of mRNA secondary structures on ribosome conformational dynamics.” 2016. Thesis, University of Missouri – Columbia. Accessed January 22, 2021. http://hdl.handle.net/10355/62520.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Shebl, Bassem. “The influence of mRNA secondary structures on ribosome conformational dynamics.” 2016. Web. 22 Jan 2021.

Vancouver:

Shebl B. The influence of mRNA secondary structures on ribosome conformational dynamics. [Internet] [Thesis]. University of Missouri – Columbia; 2016. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/10355/62520.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Shebl B. The influence of mRNA secondary structures on ribosome conformational dynamics. [Thesis]. University of Missouri – Columbia; 2016. Available from: http://hdl.handle.net/10355/62520

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Lethbridge

7. University of Lethbridge. Faculty of Arts and Science. Elucidating the role of eukaryotic initiation factor 5B (eIF5B) in non-canonical translation initiation .

Degree: 2019, University of Lethbridge

URL: http://hdl.handle.net/10133/5666

► Cap-dependent translation drives the global synthesis of proteins. Under stress conditions global protein production is attenuated, yet the translation of ATF4 is upregulated through uORF-… (more)

Subjects/Keywords: Messenger RNA; Proteins – Synthesis; Ribosomes

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APA (6^th Edition):

Science, U. o. L. F. o. A. a. (2019). Elucidating the role of eukaryotic initiation factor 5B (eIF5B) in non-canonical translation initiation . (Thesis). University of Lethbridge. Retrieved from http://hdl.handle.net/10133/5666

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Science, University of Lethbridge. Faculty of Arts and. “Elucidating the role of eukaryotic initiation factor 5B (eIF5B) in non-canonical translation initiation .” 2019. Thesis, University of Lethbridge. Accessed January 22, 2021. http://hdl.handle.net/10133/5666.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Science, University of Lethbridge. Faculty of Arts and. “Elucidating the role of eukaryotic initiation factor 5B (eIF5B) in non-canonical translation initiation .” 2019. Web. 22 Jan 2021.

Vancouver:

Science UoLFoAa. Elucidating the role of eukaryotic initiation factor 5B (eIF5B) in non-canonical translation initiation . [Internet] [Thesis]. University of Lethbridge; 2019. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/10133/5666.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Science UoLFoAa. Elucidating the role of eukaryotic initiation factor 5B (eIF5B) in non-canonical translation initiation . [Thesis]. University of Lethbridge; 2019. Available from: http://hdl.handle.net/10133/5666

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Hong Kong

8. Zhang, Yinfeng. Protein chemical synthesis by serine and threonine ligation.

Degree: 2014, University of Hong Kong

URL: http://hdl.handle.net/10722/202359

► Landmark advances in the field of synthetic protein chemistry have enabled the preparation of complex, homogeneous proteins, including those that carry specific posttranslational modifications (PTMs).… (more)

▼ Landmark advances in the field of synthetic protein chemistry have enabled the preparation of complex, homogeneous proteins, including those that carry specific posttranslational modifications (PTMs). In addition, chemical synthesis will allow one to incorporate unnatural elements to generate new biologics with altered properties and functions. Native chemical ligation (NCL) is a milestone in the chemical synthesis of proteins (Kent et al., Science, 1994, 266, 776-779), in which a C-terminal peptide thioester and an N-terminal cysteine (Cys)-containing peptide-both in side-chain unprotected forms-are selectively coupled to generate a natural peptidic linkage at the site of ligation. This method requires a cysteine at the optimal convergent ligation site. However, Cys is one of the least abundant amino acids in natural proteins. Therefore, the development of new ligation methods at other amino acids will be necessary and important in this regard. Along these lines, our laboratory has developed a novel thiol-independent approach-serine/threonine ligation (STL). It uses the N-terminal serine or threonine of a peptide segment to chemoselectively react with another peptide segment with a C-terminal salicylaldehyde ester to form an N,O-benzylidene acetal linked product, followed by acidolysis to afford the final product at the natural Ser/Thr site. To extend the application of STL in chemical protein synthesis, we have developed a robust method for the preparation of peptide salicylaldehyde esters via Fmoc-based solid phase peptide synthesis. Furthermore, we have successfully applied this ligation method in the convergent synthesis of peptide drugs of significant therapeutic importance, including Teriparatide (Forteo), Corticorelin (oCRH), Exenatide (Byetta) and Tesamorelin (hGHRH). Of significance, we have demonstrated the effectiveness of our STL in the assembly of a more complex target of biological interest: human erythrocyte acylphosphatase (~ 11 kDa). In summary, we have developed a new serine/threonine ligation, which can be effectively used to synthesize peptides and proteins. As there are countless serine and threonine residues in natural proteins, particularly those carrying posttranslational modifications, this method is anticipated to offer new opportunities in synthetic protein chemistry and chemical biology. Advisors/Committee Members: Li, XC (advisor).

Subjects/Keywords: Proteins - Synthesis

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APA (6^th Edition):

Zhang, Y. (2014). Protein chemical synthesis by serine and threonine ligation. (Thesis). University of Hong Kong. Retrieved from http://hdl.handle.net/10722/202359

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Zhang, Yinfeng. “Protein chemical synthesis by serine and threonine ligation.” 2014. Thesis, University of Hong Kong. Accessed January 22, 2021. http://hdl.handle.net/10722/202359.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Zhang, Yinfeng. “Protein chemical synthesis by serine and threonine ligation.” 2014. Web. 22 Jan 2021.

Vancouver:

Zhang Y. Protein chemical synthesis by serine and threonine ligation. [Internet] [Thesis]. University of Hong Kong; 2014. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/10722/202359.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Zhang Y. Protein chemical synthesis by serine and threonine ligation. [Thesis]. University of Hong Kong; 2014. Available from: http://hdl.handle.net/10722/202359

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Arizona

9. Ehler, Kenneth Walter, 1946-. PROGRESS TOWARD THE CHEMICAL SYNTHESIS OF PROTEINS .

Degree: 1972, University of Arizona

URL: http://hdl.handle.net/10150/287878

Subjects/Keywords: Proteins – Synthesis.

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ehler, Kenneth Walter, 1. (1972). PROGRESS TOWARD THE CHEMICAL SYNTHESIS OF PROTEINS . (Doctoral Dissertation). University of Arizona. Retrieved from http://hdl.handle.net/10150/287878

Chicago Manual of Style (16^th Edition):

Ehler, Kenneth Walter, 1946-. “PROGRESS TOWARD THE CHEMICAL SYNTHESIS OF PROTEINS .” 1972. Doctoral Dissertation, University of Arizona. Accessed January 22, 2021. http://hdl.handle.net/10150/287878.

MLA Handbook (7^th Edition):

Ehler, Kenneth Walter, 1946-. “PROGRESS TOWARD THE CHEMICAL SYNTHESIS OF PROTEINS .” 1972. Web. 22 Jan 2021.

Vancouver:

Ehler, Kenneth Walter 1. PROGRESS TOWARD THE CHEMICAL SYNTHESIS OF PROTEINS . [Internet] [Doctoral dissertation]. University of Arizona; 1972. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/10150/287878.

Council of Science Editors:

Ehler, Kenneth Walter 1. PROGRESS TOWARD THE CHEMICAL SYNTHESIS OF PROTEINS . [Doctoral Dissertation]. University of Arizona; 1972. Available from: http://hdl.handle.net/10150/287878

Massey University

10. Prescott, Hayley Dawn. Identification of potential Gcn2 regulating proteins.

Degree: MS, Genetics, 2016, Massey University

URL: http://hdl.handle.net/10179/10014

► The viability of any organism relies greatly on their ability to adapt their metabolic processes in response to environmental stimuli. Proteins are essential for almost… (more)

▼ The viability of any organism relies greatly on their ability to adapt their metabolic processes in response to environmental stimuli. Proteins are essential for almost every intracellular biological process. Proteins are composed of amino acid precursors and the levels of amino acids within the cell available for protein synthesis requires careful monitoring. Amino acid conservation needs to be initiated in response to declining availability. Under conditions of amino acid deprivation, cessation of non-essential protein synthesis and activation of amino acid biosynthetic pathways is initiated. This regulatory mechanism is referred to as the General Amino Acid Control (GAAC) pathway. General control non-derepressible 2 (Gcn2) is a fundamental constituent of the GAAC response pathway. Gcn2 senses a decline in amino acid availability and initiates the stress response by phosphorylating the alpha subunit of eukaryotic translation initiation factor 2 (eIF2a). The phosphorylation of eIF2a triggers a sequence of events resulting in increased translation of the transcriptional activator Gcn4, which subsequently induces selective expression of genes necessary for de novo amino acid synthesis. The activation and activity of Gcn2 is moderated by inhibitory and facilitative protein interactions. Published large scale purification studies identified many novel Gcn2 binding partners, some of which may function in Gcn2 regulation. However known Gcn2 regulators were absent from these datasets indicating that they were incomplete. This work aimed to identify and screen potential Gcn2 binding partners for those that regulate Gcn2 activity. Analysis identified 135 proteins that were potentially in complex with Gcn2. Of those, Sse1, Chs5, Ncl1, Tir4 and Npr1 were subsequently identified as potential Gcn2 regulators. For the purpose of comprehensively identifying novel Gcn2 binding proteins, a protocol was successfully optimised to enable Gcn2 affinity purification under conditions that would be specifically conducive for the maintenance of bonds between Gcn2 and its interaction partners. In this method, Gcn2 was overexpressed in cells to drive weak and/or transient interactions, and the usage of formaldehyde to crosslink interactions and further stabilise them was explored. This method will enable the future compilation of a comprehensive Gcn2 interactome.

Subjects/Keywords: Proteins; Synthesis; Protein-protein interactions

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APA (6^th Edition):

Prescott, H. D. (2016). Identification of potential Gcn2 regulating proteins. (Masters Thesis). Massey University. Retrieved from http://hdl.handle.net/10179/10014

Chicago Manual of Style (16^th Edition):

Prescott, Hayley Dawn. “Identification of potential Gcn2 regulating proteins.” 2016. Masters Thesis, Massey University. Accessed January 22, 2021. http://hdl.handle.net/10179/10014.

MLA Handbook (7^th Edition):

Prescott, Hayley Dawn. “Identification of potential Gcn2 regulating proteins.” 2016. Web. 22 Jan 2021.

Vancouver:

Prescott HD. Identification of potential Gcn2 regulating proteins. [Internet] [Masters thesis]. Massey University; 2016. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/10179/10014.

Council of Science Editors:

Prescott HD. Identification of potential Gcn2 regulating proteins. [Masters Thesis]. Massey University; 2016. Available from: http://hdl.handle.net/10179/10014

Massey University

11. Visser, Danielle Renee. Unravelling the molecular contributions to collagen higher order structure.

Degree: MS, Biochemistry, 2019, Massey University

URL: http://hdl.handle.net/10179/15535

► Abnormal levels of cross-linking in fibrillar collagen strands have been shown to cause a number of human and animal diseases. Cross-linking is a vital step… (more)

▼ Abnormal levels of cross-linking in fibrillar collagen strands have been shown to cause a number of human and animal diseases. Cross-linking is a vital step in fibrillogenesis and contributes greatly to the structural integrity of collagenous tissues. Conversely, defects in cross-link formation can significantly alter fibrillar organisation and lead to pathogenesis. Because collagen cross-links form on collagen-specific hydroxylated lysine residues, an understanding of the link between hydroxylysine and cross-link concentrations is needed to determine whether the level of hydroxylysine, the stereochemistry of these hydroxylysine residues, or other post-translational modifications such as glycosylation affect the level of cross-linking in tissue. While some research has been done to elucidate the connection between the two in different tissue types from the same animal, little has been undertaken to relate hydroxylation and glycosylation of lysine and hydroxylysine to the concentration and types of cross-links in different species. Furthermore, no research has been done to compare the relative distribution of diastereomers of hydroxylysine even within the same species. In order to make a valid comparison, collagen needs to be purified from skin to a high degree and separated into different collagen types and sub- structures as much as possible. To achieve this, the extraction and purification of collagen from the skins of four different mammalian species displaying different skin tensile strengths has been optimised. Different extraction methods were used to prevent the loss of specific features of the collagens that were characterised that may otherwise be lost. Amino acid analysis revealed that while the ratios of the two hydroxylysine diastereomers differed between different animals and extraction methods, the differences were not significant. Mass spectral analysis of cross-links showed that goat skin differed from the other three animals in its cross-link profile. Amino acid analysis combined with mass spectral analysis revealed that on average 70% of proline residues were hydroxylated, a figure much higher than previously thought. Mass spectral analysis also revealed that there are some differences between the glycosylation pattern of different animals, and the ratios of the different types of collagen which are extracted from each animal. While these findings need to be confirmed, they challenge some long held beliefs about the collagen molecule and provide a firm foundation for future work.

Subjects/Keywords: Collagen; Structure; Synthesis; Proteins; Crosslinking

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APA (6^th Edition):

Visser, D. R. (2019). Unravelling the molecular contributions to collagen higher order structure. (Masters Thesis). Massey University. Retrieved from http://hdl.handle.net/10179/15535

Chicago Manual of Style (16^th Edition):

Visser, Danielle Renee. “Unravelling the molecular contributions to collagen higher order structure.” 2019. Masters Thesis, Massey University. Accessed January 22, 2021. http://hdl.handle.net/10179/15535.

MLA Handbook (7^th Edition):

Visser, Danielle Renee. “Unravelling the molecular contributions to collagen higher order structure.” 2019. Web. 22 Jan 2021.

Vancouver:

Visser DR. Unravelling the molecular contributions to collagen higher order structure. [Internet] [Masters thesis]. Massey University; 2019. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/10179/15535.

Council of Science Editors:

Visser DR. Unravelling the molecular contributions to collagen higher order structure. [Masters Thesis]. Massey University; 2019. Available from: http://hdl.handle.net/10179/15535

Rutgers University

12. Mendez-Rivera, Letzibeth, 1990-. The Slx5 paradox: expression of a sumo-targeted ubiquitin ligase generates toxic poly-sumo chains in yeast.

Degree: MS, Biochemistry, 2015, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/46394/

► Defects in DNA repair are associated with a variety of human diseases, but the pathways that regulate DNA repair are poorly understood. Protein modification by… (more)

▼ Defects in DNA repair are associated with a variety of human diseases, but the pathways that regulate DNA repair are poorly understood. Protein modification by SUMO remains one of the most elusive mechanisms, even though it is known to be important for genome stability. Sgs1, a RecQ DNA helicase in S. cerevisiae, is important for DNA repair, and genetic screens have shown that the heterodimeric Slx5-Slx8 SUMO-targeted Ub ligase (STUbL) is required for cell viability in its absence. Another factor that genetically connects SUMO to DNA repair is the metalloprotease Wss1. The growth of a wss1Δ sgs1Δ double mutant is reduced compared to either single mutant. Taking a candidate-gene over-expression (OE) approach, I made the unexpected finding that SLX5 over-expression is lethal to cells lacking WSS1. Surprisingly, this lethality did not require its partner protein Slx8, which suggested that this was a novel Slx5 activity. Consistent with this lethality, was also observed when STUbLs such as yeast ULS1 or mammalian RNF4 were expressed in these cells. Further, structure-function analysis showed that lethality due to SLX5 OE required only Slx5’s SUMO interaction Motif’s (SIMs), not its RING domain. Kinetic analysis indicated that SLX5 OE slows the growth of wild-type cells. In contrast, SLX5 OE permanently arrests the growth of wss1∆ cells. While investigating the cause of this lethality, I found that expression of Slx5 SIMs resulted in the accumulation of high molecular weight SUMO conjugates in both wild-type and mutant cells. Interestingly, subsequent repression of Slx5 led to a reduction in the levels of induced poly-SUMO chains and this turnover was more rapid in wild-type than in wss1∆ cells. The fact that the accumulation of poly-SUMO chains required the Ubc9 SUMO conjugating enzyme, but not the Ulp1 or Ulp2 isopeptidases, supports the hypothesis that the SIMs actively promote chain synthesis. Finally, I found that over-expression of SUMO counteracts the lethality of SLX5 OE. This suggests that wss1∆ cells may suffer lethal damage due to a shortage of monomeric SUMO. Advisors/Committee Members: Brill, Steven J. (chair), Madura, Kiran (internal member), Walworth, Nancy (internal member), Bunting, Samuel (internal member).

Subjects/Keywords: Proteins – Synthesis; DNA repair

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APA (6^th Edition):

Mendez-Rivera, Letzibeth, 1. (2015). The Slx5 paradox: expression of a sumo-targeted ubiquitin ligase generates toxic poly-sumo chains in yeast. (Masters Thesis). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/46394/

Chicago Manual of Style (16^th Edition):

Mendez-Rivera, Letzibeth, 1990-. “The Slx5 paradox: expression of a sumo-targeted ubiquitin ligase generates toxic poly-sumo chains in yeast.” 2015. Masters Thesis, Rutgers University. Accessed January 22, 2021. https://rucore.libraries.rutgers.edu/rutgers-lib/46394/.

MLA Handbook (7^th Edition):

Mendez-Rivera, Letzibeth, 1990-. “The Slx5 paradox: expression of a sumo-targeted ubiquitin ligase generates toxic poly-sumo chains in yeast.” 2015. Web. 22 Jan 2021.

Vancouver:

Mendez-Rivera, Letzibeth 1. The Slx5 paradox: expression of a sumo-targeted ubiquitin ligase generates toxic poly-sumo chains in yeast. [Internet] [Masters thesis]. Rutgers University; 2015. [cited 2021 Jan 22]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/46394/.

Council of Science Editors:

Mendez-Rivera, Letzibeth 1. The Slx5 paradox: expression of a sumo-targeted ubiquitin ligase generates toxic poly-sumo chains in yeast. [Masters Thesis]. Rutgers University; 2015. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/46394/

University of Hong Kong

13. Tang, Chi-wai, Sydney. Human kidney as an organ of complement synthesis: its regulation by tubular protein.

Degree: 2001, University of Hong Kong

URL: http://hdl.handle.net/10722/27597

Subjects/Keywords: Proteins - Synthesis.; Kidneys.

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APA (6^th Edition):

Tang, C. (2001). Human kidney as an organ of complement synthesis: its regulation by tubular protein. (Thesis). University of Hong Kong. Retrieved from http://hdl.handle.net/10722/27597

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Tang, Chi-wai,. “Human kidney as an organ of complement synthesis: its regulation by tubular protein.” 2001. Thesis, University of Hong Kong. Accessed January 22, 2021. http://hdl.handle.net/10722/27597.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Tang, Chi-wai,. “Human kidney as an organ of complement synthesis: its regulation by tubular protein.” 2001. Web. 22 Jan 2021.

Vancouver:

Tang C. Human kidney as an organ of complement synthesis: its regulation by tubular protein. [Internet] [Thesis]. University of Hong Kong; 2001. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/10722/27597.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Tang C. Human kidney as an organ of complement synthesis: its regulation by tubular protein. [Thesis]. University of Hong Kong; 2001. Available from: http://hdl.handle.net/10722/27597

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Columbia University

14. Hornstein, Nicholas James. Defining Protein Synthesis: New Technologies to Elucidate Translational Control.

Degree: 2017, Columbia University

URL: https://doi.org/10.7916/D8ZG74J1

► Protein translation has emerged as an important mediator of cellular activity. Here, we discuss efforts to develop and apply new technologies designed to gain insights… (more)

▼ Protein translation has emerged as an important mediator of cellular activity. Here, we discuss efforts to develop and apply new technologies designed to gain insights into translational control. We begin with the application of ribosome profiling to a RiboTag Glioma mouse model which enables translational profiling of transformed cellular populations. This approach demonstrates a number of abnormalities of translation in transformed cells. We go on to report the development of an inexpensive and rapid library preparation methodology which enables high-throughput sequencing of ribosome-protected footprints from small amounts of input material. We apply this technique to a CAMKII RiboTag mouse model to make new insights into cell-type specific translation. Finally, we describe efforts to investigate translation regulatory networks through the development of a technique which couples large-scale perturbation with a genome-wide readout of translation. Molecular dissection of tissues through the ectopic expression of modified ribosomal proteins commonly relies on tissue-specific genes which act as drivers. In the case of glioma, a gene specific to transformed tissue, but not expressed in normal brain tissue, has not been identified. Chapter 2 focuses on efforts to bypass this through the development of a RiboTag Glioma mouse model which allows for concurrent transformation and the expression of an epitope-tagged ribosomal protein in virally infected cells. This model made possible the isolation of translating mRNA from transformed cellular populations and was used to demonstrate the existence of a number of translational abnormalities in transformed cells. Conventional ribosome profiling is a powerful tool which allows for the identification of ribosome-protected mRNA footprints. However, it is time-consuming, expensive, and difficult to implement. Based on our experiences with conventional ribosome profiling, we sought to develop a method which could decrease the overall number of enzymatic reactions and purification steps, thereby reducing the time and cost associated with the procedure; these efforts are discussed in Chapter 3. Utilizing a ligation-free library preparation process, which incorporates poly(A)-polymerase, template switching and bead-based purification, we reduced the time, costs and input requirements required to generate a ribosome profiling library while maintaining high library complexity. We applied our ligation-free ribosome profiling technique to a CAMKII RiboTag mouse model which enabled us to identify patterns of cell-type specific translation and the effects of mTOR inhibition in CAMKII-expressing excitatory neurons. Regulation of protein expression is an essential and highly complex cellular activity. Aberrations of translational control are central to a host of pathologies and have direct clinical relevance. However, our knowledge of the networks which control translation is limited. Chapter 4 details our efforts to develop a highly-scalable technology which enables the…

Subjects/Keywords: Biology – Classification; Proteins – Synthesis; Genetic translation

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APA (6^th Edition):

Hornstein, N. J. (2017). Defining Protein Synthesis: New Technologies to Elucidate Translational Control. (Doctoral Dissertation). Columbia University. Retrieved from https://doi.org/10.7916/D8ZG74J1

Chicago Manual of Style (16^th Edition):

Hornstein, Nicholas James. “Defining Protein Synthesis: New Technologies to Elucidate Translational Control.” 2017. Doctoral Dissertation, Columbia University. Accessed January 22, 2021. https://doi.org/10.7916/D8ZG74J1.

MLA Handbook (7^th Edition):

Hornstein, Nicholas James. “Defining Protein Synthesis: New Technologies to Elucidate Translational Control.” 2017. Web. 22 Jan 2021.

Vancouver:

Hornstein NJ. Defining Protein Synthesis: New Technologies to Elucidate Translational Control. [Internet] [Doctoral dissertation]. Columbia University; 2017. [cited 2021 Jan 22]. Available from: https://doi.org/10.7916/D8ZG74J1.

Council of Science Editors:

Hornstein NJ. Defining Protein Synthesis: New Technologies to Elucidate Translational Control. [Doctoral Dissertation]. Columbia University; 2017. Available from: https://doi.org/10.7916/D8ZG74J1

Michigan State University

15. Evins, Warren H., 1944-. Hormonal regulation of protein synthesis in barley aleurone layers.

Degree: PhD, 1970, Michigan State University

URL: http://etd.lib.msu.edu/islandora/object/etd:39914

Subjects/Keywords: Proteins – Synthesis; Barley

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APA (6^th Edition):

Evins, Warren H., 1. (1970). Hormonal regulation of protein synthesis in barley aleurone layers. (Doctoral Dissertation). Michigan State University. Retrieved from http://etd.lib.msu.edu/islandora/object/etd:39914

Chicago Manual of Style (16^th Edition):

Evins, Warren H., 1944-. “Hormonal regulation of protein synthesis in barley aleurone layers.” 1970. Doctoral Dissertation, Michigan State University. Accessed January 22, 2021. http://etd.lib.msu.edu/islandora/object/etd:39914.

MLA Handbook (7^th Edition):

Evins, Warren H., 1944-. “Hormonal regulation of protein synthesis in barley aleurone layers.” 1970. Web. 22 Jan 2021.

Vancouver:

Evins, Warren H. 1. Hormonal regulation of protein synthesis in barley aleurone layers. [Internet] [Doctoral dissertation]. Michigan State University; 1970. [cited 2021 Jan 22]. Available from: http://etd.lib.msu.edu/islandora/object/etd:39914.

Council of Science Editors:

Evins, Warren H. 1. Hormonal regulation of protein synthesis in barley aleurone layers. [Doctoral Dissertation]. Michigan State University; 1970. Available from: http://etd.lib.msu.edu/islandora/object/etd:39914

Michigan State University

16. Casjens, Sherwood Reid. The selective inhibition of protein assembly by gougerotin.

Degree: MS, Department of Biochemistry, 1967, Michigan State University

URL: http://etd.lib.msu.edu/islandora/object/etd:40454

Subjects/Keywords: Gougerotin; Proteins – Synthesis

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APA (6^th Edition):

Casjens, S. R. (1967). The selective inhibition of protein assembly by gougerotin. (Masters Thesis). Michigan State University. Retrieved from http://etd.lib.msu.edu/islandora/object/etd:40454

Chicago Manual of Style (16^th Edition):

Casjens, Sherwood Reid. “The selective inhibition of protein assembly by gougerotin.” 1967. Masters Thesis, Michigan State University. Accessed January 22, 2021. http://etd.lib.msu.edu/islandora/object/etd:40454.

MLA Handbook (7^th Edition):

Casjens, Sherwood Reid. “The selective inhibition of protein assembly by gougerotin.” 1967. Web. 22 Jan 2021.

Vancouver:

Casjens SR. The selective inhibition of protein assembly by gougerotin. [Internet] [Masters thesis]. Michigan State University; 1967. [cited 2021 Jan 22]. Available from: http://etd.lib.msu.edu/islandora/object/etd:40454.

Council of Science Editors:

Casjens SR. The selective inhibition of protein assembly by gougerotin. [Masters Thesis]. Michigan State University; 1967. Available from: http://etd.lib.msu.edu/islandora/object/etd:40454

Montana State University

17. Roessner, Charles Alexander. The partial purification and characterization of a ribosomal wash factor required for the translation of polycytidylate.

Degree: PhD, College of Agriculture, 1976, Montana State University

URL: https://scholarworks.montana.edu/xmlui/handle/1/4533

Subjects/Keywords: Proteins Synthesis.; Ribosomes.

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APA (6^th Edition):

Roessner, C. A. (1976). The partial purification and characterization of a ribosomal wash factor required for the translation of polycytidylate. (Doctoral Dissertation). Montana State University. Retrieved from https://scholarworks.montana.edu/xmlui/handle/1/4533

Chicago Manual of Style (16^th Edition):

Roessner, Charles Alexander. “The partial purification and characterization of a ribosomal wash factor required for the translation of polycytidylate.” 1976. Doctoral Dissertation, Montana State University. Accessed January 22, 2021. https://scholarworks.montana.edu/xmlui/handle/1/4533.

MLA Handbook (7^th Edition):

Roessner, Charles Alexander. “The partial purification and characterization of a ribosomal wash factor required for the translation of polycytidylate.” 1976. Web. 22 Jan 2021.

Vancouver:

Roessner CA. The partial purification and characterization of a ribosomal wash factor required for the translation of polycytidylate. [Internet] [Doctoral dissertation]. Montana State University; 1976. [cited 2021 Jan 22]. Available from: https://scholarworks.montana.edu/xmlui/handle/1/4533.

Council of Science Editors:

Roessner CA. The partial purification and characterization of a ribosomal wash factor required for the translation of polycytidylate. [Doctoral Dissertation]. Montana State University; 1976. Available from: https://scholarworks.montana.edu/xmlui/handle/1/4533

Montana State University

18. Green, Charles Edward. Mechanism of action of chloramphenicol : effect on T4D head coat protein synthesis.

Degree: PhD, College of Agriculture, 1970, Montana State University

URL: https://scholarworks.montana.edu/xmlui/handle/1/4334

Subjects/Keywords: Chloramphenicol.; Proteins Synthesis.

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APA (6^th Edition):

Green, C. E. (1970). Mechanism of action of chloramphenicol : effect on T4D head coat protein synthesis. (Doctoral Dissertation). Montana State University. Retrieved from https://scholarworks.montana.edu/xmlui/handle/1/4334

Chicago Manual of Style (16^th Edition):

Green, Charles Edward. “Mechanism of action of chloramphenicol : effect on T4D head coat protein synthesis.” 1970. Doctoral Dissertation, Montana State University. Accessed January 22, 2021. https://scholarworks.montana.edu/xmlui/handle/1/4334.

MLA Handbook (7^th Edition):

Green, Charles Edward. “Mechanism of action of chloramphenicol : effect on T4D head coat protein synthesis.” 1970. Web. 22 Jan 2021.

Vancouver:

Green CE. Mechanism of action of chloramphenicol : effect on T4D head coat protein synthesis. [Internet] [Doctoral dissertation]. Montana State University; 1970. [cited 2021 Jan 22]. Available from: https://scholarworks.montana.edu/xmlui/handle/1/4334.

Council of Science Editors:

Green CE. Mechanism of action of chloramphenicol : effect on T4D head coat protein synthesis. [Doctoral Dissertation]. Montana State University; 1970. Available from: https://scholarworks.montana.edu/xmlui/handle/1/4334

University of Arizona

19. Greene, Robert Alva, 1905-. Studies in protein synthesis by the genus Azotobacter .

Degree: 1933, University of Arizona

URL: http://hdl.handle.net/10150/565091

Subjects/Keywords: Azotobacter.; Proteins – Synthesis.

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APA (6^th Edition):

Greene, Robert Alva, 1. (1933). Studies in protein synthesis by the genus Azotobacter . (Doctoral Dissertation). University of Arizona. Retrieved from http://hdl.handle.net/10150/565091

Chicago Manual of Style (16^th Edition):

Greene, Robert Alva, 1905-. “Studies in protein synthesis by the genus Azotobacter .” 1933. Doctoral Dissertation, University of Arizona. Accessed January 22, 2021. http://hdl.handle.net/10150/565091.

MLA Handbook (7^th Edition):

Greene, Robert Alva, 1905-. “Studies in protein synthesis by the genus Azotobacter .” 1933. Web. 22 Jan 2021.

Vancouver:

Greene, Robert Alva 1. Studies in protein synthesis by the genus Azotobacter . [Internet] [Doctoral dissertation]. University of Arizona; 1933. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/10150/565091.

Council of Science Editors:

Greene, Robert Alva 1. Studies in protein synthesis by the genus Azotobacter . [Doctoral Dissertation]. University of Arizona; 1933. Available from: http://hdl.handle.net/10150/565091

University of Arizona

20. Vessey, Donald A. RELATIONSHIPS BETWEEN MESSENGER-RNA, POLYRIBOSOMES AND PROTEIN SYNTHESIS IN A YEAST SPHEROPLAST SYSTEM .

Degree: 1970, University of Arizona

URL: http://hdl.handle.net/10150/287518

Subjects/Keywords: Proteins – Synthesis.; Ribosomes.

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APA (6^th Edition):

Vessey, D. A. (1970). RELATIONSHIPS BETWEEN MESSENGER-RNA, POLYRIBOSOMES AND PROTEIN SYNTHESIS IN A YEAST SPHEROPLAST SYSTEM . (Doctoral Dissertation). University of Arizona. Retrieved from http://hdl.handle.net/10150/287518

Chicago Manual of Style (16^th Edition):

Vessey, Donald A. “RELATIONSHIPS BETWEEN MESSENGER-RNA, POLYRIBOSOMES AND PROTEIN SYNTHESIS IN A YEAST SPHEROPLAST SYSTEM .” 1970. Doctoral Dissertation, University of Arizona. Accessed January 22, 2021. http://hdl.handle.net/10150/287518.

MLA Handbook (7^th Edition):

Vessey, Donald A. “RELATIONSHIPS BETWEEN MESSENGER-RNA, POLYRIBOSOMES AND PROTEIN SYNTHESIS IN A YEAST SPHEROPLAST SYSTEM .” 1970. Web. 22 Jan 2021.

Vancouver:

Vessey DA. RELATIONSHIPS BETWEEN MESSENGER-RNA, POLYRIBOSOMES AND PROTEIN SYNTHESIS IN A YEAST SPHEROPLAST SYSTEM . [Internet] [Doctoral dissertation]. University of Arizona; 1970. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/10150/287518.

Council of Science Editors:

Vessey DA. RELATIONSHIPS BETWEEN MESSENGER-RNA, POLYRIBOSOMES AND PROTEIN SYNTHESIS IN A YEAST SPHEROPLAST SYSTEM . [Doctoral Dissertation]. University of Arizona; 1970. Available from: http://hdl.handle.net/10150/287518

University of Arizona

21. Rhodes, Patsy Ruth. POLYRIBOSOME CONTENT AND IN VITRO PROTEIN SYNTHESIS STUDIES OF EXTRACTS FROM PLANT TISSUES UNDERGOING WATER STRESS .

Degree: 1978, University of Arizona

URL: http://hdl.handle.net/10150/298840

Subjects/Keywords: Ribosomes.; Proteins – Synthesis.

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APA (6^th Edition):

Rhodes, P. R. (1978). POLYRIBOSOME CONTENT AND IN VITRO PROTEIN SYNTHESIS STUDIES OF EXTRACTS FROM PLANT TISSUES UNDERGOING WATER STRESS . (Doctoral Dissertation). University of Arizona. Retrieved from http://hdl.handle.net/10150/298840

Chicago Manual of Style (16^th Edition):

Rhodes, Patsy Ruth. “POLYRIBOSOME CONTENT AND IN VITRO PROTEIN SYNTHESIS STUDIES OF EXTRACTS FROM PLANT TISSUES UNDERGOING WATER STRESS .” 1978. Doctoral Dissertation, University of Arizona. Accessed January 22, 2021. http://hdl.handle.net/10150/298840.

MLA Handbook (7^th Edition):

Rhodes, Patsy Ruth. “POLYRIBOSOME CONTENT AND IN VITRO PROTEIN SYNTHESIS STUDIES OF EXTRACTS FROM PLANT TISSUES UNDERGOING WATER STRESS .” 1978. Web. 22 Jan 2021.

Vancouver:

Rhodes PR. POLYRIBOSOME CONTENT AND IN VITRO PROTEIN SYNTHESIS STUDIES OF EXTRACTS FROM PLANT TISSUES UNDERGOING WATER STRESS . [Internet] [Doctoral dissertation]. University of Arizona; 1978. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/10150/298840.

Council of Science Editors:

Rhodes PR. POLYRIBOSOME CONTENT AND IN VITRO PROTEIN SYNTHESIS STUDIES OF EXTRACTS FROM PLANT TISSUES UNDERGOING WATER STRESS . [Doctoral Dissertation]. University of Arizona; 1978. Available from: http://hdl.handle.net/10150/298840

McGill University

22. Baldo, Allain. Acylation stimulating protein and the regulation of intracellular triglyceride synthesis.

Degree: PhD, Department of Experimental Medicine, 1993, McGill University

URL: https://escholarship.mcgill.ca/downloads/9p290c93d.pdf ; https://escholarship.mcgill.ca/concern/theses/k930c069b

►

Acylation Stimulating Protein (ASP) a été purifié jusqu'à homogénéité et identifie comme étant C3adesArg par analyse de séquence en acides aminés N-terminales, composition en acides… (more)

Subjects/Keywords: Proteins; Triglyceride synthesis

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APA (6^th Edition):

Baldo, A. (1993). Acylation stimulating protein and the regulation of intracellular triglyceride synthesis. (Doctoral Dissertation). McGill University. Retrieved from https://escholarship.mcgill.ca/downloads/9p290c93d.pdf ; https://escholarship.mcgill.ca/concern/theses/k930c069b

Chicago Manual of Style (16^th Edition):

Baldo, Allain. “Acylation stimulating protein and the regulation of intracellular triglyceride synthesis.” 1993. Doctoral Dissertation, McGill University. Accessed January 22, 2021. https://escholarship.mcgill.ca/downloads/9p290c93d.pdf ; https://escholarship.mcgill.ca/concern/theses/k930c069b.

MLA Handbook (7^th Edition):

Baldo, Allain. “Acylation stimulating protein and the regulation of intracellular triglyceride synthesis.” 1993. Web. 22 Jan 2021.

Vancouver:

Baldo A. Acylation stimulating protein and the regulation of intracellular triglyceride synthesis. [Internet] [Doctoral dissertation]. McGill University; 1993. [cited 2021 Jan 22]. Available from: https://escholarship.mcgill.ca/downloads/9p290c93d.pdf ; https://escholarship.mcgill.ca/concern/theses/k930c069b.

Council of Science Editors:

Baldo A. Acylation stimulating protein and the regulation of intracellular triglyceride synthesis. [Doctoral Dissertation]. McGill University; 1993. Available from: https://escholarship.mcgill.ca/downloads/9p290c93d.pdf ; https://escholarship.mcgill.ca/concern/theses/k930c069b

Kansas State University

23. Müller, U. R. (Uwe R.). Bacteriophage T₄ induced modification of valyl-tRNA synthetase in Escherichia coli. : an analysis of the kinetics and regulation.

Degree: 1973, Kansas State University

URL: http://hdl.handle.net/2097/10836

Subjects/Keywords: Proteins – Synthesis; Enzymes

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APA (6^th Edition):

Müller, U. R. (. R. ). (1973). Bacteriophage T₄ induced modification of valyl-tRNA synthetase in Escherichia coli. : an analysis of the kinetics and regulation. (Thesis). Kansas State University. Retrieved from http://hdl.handle.net/2097/10836

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Müller, U R (Uwe R ). “Bacteriophage T₄ induced modification of valyl-tRNA synthetase in Escherichia coli. : an analysis of the kinetics and regulation.” 1973. Thesis, Kansas State University. Accessed January 22, 2021. http://hdl.handle.net/2097/10836.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Müller, U R (Uwe R ). “Bacteriophage T₄ induced modification of valyl-tRNA synthetase in Escherichia coli. : an analysis of the kinetics and regulation.” 1973. Web. 22 Jan 2021.

Vancouver:

Müller UR(R). Bacteriophage T₄ induced modification of valyl-tRNA synthetase in Escherichia coli. : an analysis of the kinetics and regulation. [Internet] [Thesis]. Kansas State University; 1973. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/2097/10836.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Müller UR(R). Bacteriophage T₄ induced modification of valyl-tRNA synthetase in Escherichia coli. : an analysis of the kinetics and regulation. [Thesis]. Kansas State University; 1973. Available from: http://hdl.handle.net/2097/10836

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Massey University

24. Manderson, Daniel. Development of optimal fermentation expression systems for recombinant proteins: a thesis submitted in partial fulfilment of the requirements for the degree of Masters in Technology at Massey University .

Degree: 2004, Massey University

URL: http://hdl.handle.net/10179/12019

► This research set out to maximise the titre of four recombinant protein products (i.e. Eg95 vaccine antigen against Echinococcus granulosus a aspartyl protease inhibitor homologue,… (more)

▼ This research set out to maximise the titre of four recombinant protein products (i.e. Eg95 vaccine antigen against Echinococcus granulosus a aspartyl protease inhibitor homologue, Aspin; a secreted cytokine granulocyte colony stimulating factor (G-CSF); a secreted gonadotropin ovine follicle stimulating factor (oFSH)) and develop parameters for the expression of those proteins in a small scale stirred tank biorcactor. Production of Eg95 as inclusion bodies in E. coli was influenced by the medium, feeding strategy, induction timing and dissolved oxygen concentration. Expression was greatest using the medium Terrific Broth. Higher Eg95 titres were favoured using exponential feeding, a low dissolved oxygen concentration and with cells induced in mid-exponential growth. A maximum titre of 1.73 g/L of Eg95 was produced in a fed-batch fermentation controlled at 37°C, pH 7.0 and 30% dissolved oxygen. Induction with 0.1 mM of IPTG added four hours after inoculation, was optimal. The maximum titre attained, was a 360% improvement on fermentations prior to this research. Aspin was used to investigate the culture conditions for maximizing the production of soluble protein in E.coli. Soluble Aspin production was favoured at low expression rates. A volumetric titre of 0.220 g/L of soluble Aspin was attained in batch fermentation by inducing with 2 g/L of L-arabinose, with the temperature reduced from 37°C to 23°C and by maintaining a low dissolved oxygen (DO) concentration. This yield was relatively high compared to previous reports [1-3]. G-CSF production in the yeast Pichia pastoris was influenced by the medium, pH and methanol-to-cell ratio. A maximum titre of 0.028g/L of G-CSF was produced in shaker flasks of enhanced yeast extract Hy-Soy dextrose medium (YEHD), maintained at 200 rpm, 30°C, pH 6.0 and with 1% (v/v) methanol fed per day. Cells were resuspended to an optical density of 8 prior to induction. No improvement in G-CSF was achieved in the fermenter, likely due to an inhibition by toxic materials. The optimised shaker flask yield was consistent with previous reports [4-6]. Production of oFSH in insect cells was influenced by the cell density at inoculation and rate of agitation. 0.001 g/L of oFSH was produced in shaker flasks inoculated at a density of 1 x 106 cells/mL, cultured at 27°C and agitated at 140rpm. This represented an improvement over previous yields[7].

Subjects/Keywords: Recombinant proteins – Synthesis

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APA (6^th Edition):

Manderson, D. (2004). Development of optimal fermentation expression systems for recombinant proteins: a thesis submitted in partial fulfilment of the requirements for the degree of Masters in Technology at Massey University . (Thesis). Massey University. Retrieved from http://hdl.handle.net/10179/12019

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Manderson, Daniel. “Development of optimal fermentation expression systems for recombinant proteins: a thesis submitted in partial fulfilment of the requirements for the degree of Masters in Technology at Massey University .” 2004. Thesis, Massey University. Accessed January 22, 2021. http://hdl.handle.net/10179/12019.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Manderson, Daniel. “Development of optimal fermentation expression systems for recombinant proteins: a thesis submitted in partial fulfilment of the requirements for the degree of Masters in Technology at Massey University .” 2004. Web. 22 Jan 2021.

Vancouver:

Manderson D. Development of optimal fermentation expression systems for recombinant proteins: a thesis submitted in partial fulfilment of the requirements for the degree of Masters in Technology at Massey University . [Internet] [Thesis]. Massey University; 2004. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/10179/12019.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Manderson D. Development of optimal fermentation expression systems for recombinant proteins: a thesis submitted in partial fulfilment of the requirements for the degree of Masters in Technology at Massey University . [Thesis]. Massey University; 2004. Available from: http://hdl.handle.net/10179/12019

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Massey University

25. Anderson, Reuben Andrew. Identification of large ribosomal proteins required for the full activation of the protein kinase Gcn2 in Saccharomyces cerevisiae.

Degree: MS, Biologocal Sciences, 2019, Massey University

URL: http://hdl.handle.net/10179/15307

► Protein synthesis is a fundamental biological process that all organisms require for maintaining life, growth and development. The maintenance of amino acid levels, the building… (more)

Subjects/Keywords: Ribosomes; Protein kinases; Saccharomyces cerevisiae; Proteins; Synthesis

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APA (6^th Edition):

Anderson, R. A. (2019). Identification of large ribosomal proteins required for the full activation of the protein kinase Gcn2 in Saccharomyces cerevisiae. (Masters Thesis). Massey University. Retrieved from http://hdl.handle.net/10179/15307

Chicago Manual of Style (16^th Edition):

Anderson, Reuben Andrew. “Identification of large ribosomal proteins required for the full activation of the protein kinase Gcn2 in Saccharomyces cerevisiae.” 2019. Masters Thesis, Massey University. Accessed January 22, 2021. http://hdl.handle.net/10179/15307.

MLA Handbook (7^th Edition):

Anderson, Reuben Andrew. “Identification of large ribosomal proteins required for the full activation of the protein kinase Gcn2 in Saccharomyces cerevisiae.” 2019. Web. 22 Jan 2021.

Vancouver:

Anderson RA. Identification of large ribosomal proteins required for the full activation of the protein kinase Gcn2 in Saccharomyces cerevisiae. [Internet] [Masters thesis]. Massey University; 2019. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/10179/15307.

Council of Science Editors:

Anderson RA. Identification of large ribosomal proteins required for the full activation of the protein kinase Gcn2 in Saccharomyces cerevisiae. [Masters Thesis]. Massey University; 2019. Available from: http://hdl.handle.net/10179/15307

University of Texas – Austin

26. Pavel, Ioana Simona. Assembly of gold nanoparticles by ribosomal molecular machines.

Degree: PhD, Chemistry, 2005, University of Texas – Austin

URL: http://hdl.handle.net/2152/1695

► The goal of this work is to develop novel method for organizing gold nanoparticles by linking them to chemically reactive side chains amino acids (e.g.,… (more)

▼ The goal of this work is to develop novel method for organizing gold nanoparticles by linking them to chemically reactive side chains amino acids (e.g., lysine and cysteine) and then incorporating these modified amino acids into a polypeptide through in vitro translation of a synthetic mRNA template. The use of this system not only provides a method for the supramolecular assembly of new bio-inorganic heterostructures, using ribosomes as natural molecular machines and an artificial coding template, but also provides a new tool for probing fundamental biological processes (e.g., translation). Transfer RNA (tRNA) is transcribed in vitro from a plasmid-template containing the tRNA gene under control of the T7 promoter. A novel coupled transcription– aminoacylation (CTA) system which prepares large amounts of pure aminoacyl-tRNA in a single reaction mixture was developed. Radiolabeled [32P]-UTP and [14C] or [32S]- amino acid are used to monitor the coupled transcription- aminoacylation reactions. The ability to further modify the tRNA with a biotinylated probe is also demonstrated. The tRNA synthesized by CTA, or further modified with biotin is shown to retain its functionality in in vitro translation, allowing the synthesis and detection of biotin-labeled protein. Following the charging of the tRNA, the side chain of the amino acid is covalently linked to a gold nanoparticle, i.e. Monomaleido or Mono-sulfo-Nhydroxysuccinimide Nanogolds, either directly or through a heterobifunctional linker, which may vary in length and/or chemical properties, depending on the system used. The result is a novel gold nanoparticle-conjugated tRNA. Each of the steps is verified by mass spectroscopy (ESI and/or MALDI). The attachment of the gold nanoparticle to modified aminoacyl-tRNA is confirmed using High angle annular dark field images (HTEM) and laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). The incorporation of the quantum dot-conjugated tRNA into a polypeptide is measured using an in vitro E. coli S30 cell-free coupled transcription-translation system, and verified by electrophoresis, laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS), High angle annular dark field (HAADF) images and electron loss spectroscopy . Advisors/Committee Members: Browning, Karen S. (advisor).

Subjects/Keywords: Proteins – Synthesis; Ribosomes

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APA (6^th Edition):

Pavel, I. S. (2005). Assembly of gold nanoparticles by ribosomal molecular machines. (Doctoral Dissertation). University of Texas – Austin. Retrieved from http://hdl.handle.net/2152/1695

Chicago Manual of Style (16^th Edition):

Pavel, Ioana Simona. “Assembly of gold nanoparticles by ribosomal molecular machines.” 2005. Doctoral Dissertation, University of Texas – Austin. Accessed January 22, 2021. http://hdl.handle.net/2152/1695.

MLA Handbook (7^th Edition):

Pavel, Ioana Simona. “Assembly of gold nanoparticles by ribosomal molecular machines.” 2005. Web. 22 Jan 2021.

Vancouver:

Pavel IS. Assembly of gold nanoparticles by ribosomal molecular machines. [Internet] [Doctoral dissertation]. University of Texas – Austin; 2005. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/2152/1695.

Council of Science Editors:

Pavel IS. Assembly of gold nanoparticles by ribosomal molecular machines. [Doctoral Dissertation]. University of Texas – Austin; 2005. Available from: http://hdl.handle.net/2152/1695

University of Texas – Austin

27. Sadow, Jennifer Beth Hurley. The x-ray crystal structure of wheat translation initiation factor eIF4E.

Degree: PhD, Biochemistry, 2002, University of Texas – Austin

URL: http://hdl.handle.net/2152/895

Subjects/Keywords: Proteins – Synthesis; Proteins – Structure; Crystallography

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sadow, J. B. H. (2002). The x-ray crystal structure of wheat translation initiation factor eIF4E. (Doctoral Dissertation). University of Texas – Austin. Retrieved from http://hdl.handle.net/2152/895

Chicago Manual of Style (16^th Edition):

Sadow, Jennifer Beth Hurley. “The x-ray crystal structure of wheat translation initiation factor eIF4E.” 2002. Doctoral Dissertation, University of Texas – Austin. Accessed January 22, 2021. http://hdl.handle.net/2152/895.

MLA Handbook (7^th Edition):

Sadow, Jennifer Beth Hurley. “The x-ray crystal structure of wheat translation initiation factor eIF4E.” 2002. Web. 22 Jan 2021.

Vancouver:

Sadow JBH. The x-ray crystal structure of wheat translation initiation factor eIF4E. [Internet] [Doctoral dissertation]. University of Texas – Austin; 2002. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/2152/895.

Council of Science Editors:

Sadow JBH. The x-ray crystal structure of wheat translation initiation factor eIF4E. [Doctoral Dissertation]. University of Texas – Austin; 2002. Available from: http://hdl.handle.net/2152/895

Nelson Mandela Metropolitan University

28. Suleman, Essa. Mutational analysis of the PacC binding sites within the aflR promoter in Aspergillus flavus.

Degree: PhD, Faculty of Science, 2011, Nelson Mandela Metropolitan University

URL: http://hdl.handle.net/10948/d1012683

► It is generally known that media containing simple sugars (sucrose, glucose) and organic nitrogen sources (ammonium) when buffered to acidic pH stimulates aflatoxin production in… (more)

▼ It is generally known that media containing simple sugars (sucrose, glucose) and organic nitrogen sources (ammonium) when buffered to acidic pH stimulates aflatoxin production in Aspergillus flavus & A. parasiticus while lactose, nitrate and an alkaline pH inhibit aflatoxin biosynthesis. It has been shown that pH of the growth medium is the most important regulatory factor for aflatoxin biosynthesis since media containing stimulatory carbon and/or nitrogen sources (sucrose and ammonia) do not enhance aflatoxin (or sterigmatocystin) production at alkaline pH. RNA interference (in A. flavus) of the pH regulatory transcription factor, PacC, resulted in aflatoxin production under acidic and alkaline pH conditions whilst wildtype Aspergillus flavus produced aflatoxins only under acidic conditions. This conclusively proved that PacC negatively regulates aflatoxin production at alkaline pH in A. flavus. However the exact mechanism involved in PacC repression of aflatoxin biosynthesis at alkaline pH still remains unknown. The AflR protein is essential for expression of several genes in the aflatoxin biosynthetic cluster. In the current study, sequence analysis of the aflR promoter indicated the presence of two putative PacC binding sites within the aflR promoter of A. flavus 3357WT located at positions -162 and -487 bp from the start codon. The presence of the PacC binding sites in the aflR promoter indicated a possible link between aflR expression and PacC regulation under alkaline conditions. Thus, in this study, it was hypothesized that at alkaline pH, PacC inhibits aflR expression by binding to one or both of the PacC binding sites within the aflR promoter. This in turn, would result in inhibition of aflatoxin biosynthesis since expression of several aflatoxin biosynthetic pathway genes is dependent on activation by AflR. The aim and objective of this study was to test the validity of this hypothesis i.e. that at alkaline pH PacC binds to one or both of its recognition sites within the aflR promoter thereby inhibiting aflR expression which subsequently would result in inhibition of aflatoxin biosynthesis. This was done by first mutating each individual and then both PacC binding sites in the A. flavus 3357 aflR promoter via Single-Joint PCR (SJ-PCR) and fusing the wildtype and each mutated aflR promoter to the Green Fluorescent Protein (gfp) gene and the trpC terminator to yield a functional expression vector. These constructs were then transformed into A. flavus 3357.5. Positive transformants were confirmed to express GFP by fluorescence microscopy and spectrofluorometry. Quantification of GFP protein levels of the various transformants in this study indicated that PacC negatively regulated aflR promoter activity at alkaline pH. RT-qPCR was performed on positive transformants after growth on SLS medium at acidic and alkaline pH to determine if PacC negatively regulated aflR promoter activity at alkaline pH and to determine whether PacC binds preferentially to one or both recognition sites within the aflR promoter.… Advisors/Committee Members: Somai, Benesh Munilal Dr.

Subjects/Keywords: Mutation (Biology); Genetic regulation; Proteins – Synthesis; Microbiological synthesis

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APA (6^th Edition):

Suleman, E. (2011). Mutational analysis of the PacC binding sites within the aflR promoter in Aspergillus flavus. (Doctoral Dissertation). Nelson Mandela Metropolitan University. Retrieved from http://hdl.handle.net/10948/d1012683

Chicago Manual of Style (16^th Edition):

Suleman, Essa. “Mutational analysis of the PacC binding sites within the aflR promoter in Aspergillus flavus.” 2011. Doctoral Dissertation, Nelson Mandela Metropolitan University. Accessed January 22, 2021. http://hdl.handle.net/10948/d1012683.

MLA Handbook (7^th Edition):

Suleman, Essa. “Mutational analysis of the PacC binding sites within the aflR promoter in Aspergillus flavus.” 2011. Web. 22 Jan 2021.

Vancouver:

Suleman E. Mutational analysis of the PacC binding sites within the aflR promoter in Aspergillus flavus. [Internet] [Doctoral dissertation]. Nelson Mandela Metropolitan University; 2011. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/10948/d1012683.

Council of Science Editors:

Suleman E. Mutational analysis of the PacC binding sites within the aflR promoter in Aspergillus flavus. [Doctoral Dissertation]. Nelson Mandela Metropolitan University; 2011. Available from: http://hdl.handle.net/10948/d1012683

Michigan State University

29. Bucholtz, Herbert Francis, 1943-. Microbial protein synthesis in the rumen : assessment of radioactive phosphorous as a marker for cellular growth.

Degree: PhD, Department of Animal Husbandry and Institute of Nutrition, 1972, Michigan State University

URL: http://etd.lib.msu.edu/islandora/object/etd:34700

Subjects/Keywords: Microbiological synthesis; Proteins – Synthesis; Nutrition

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APA (6^th Edition):

Bucholtz, Herbert Francis, 1. (1972). Microbial protein synthesis in the rumen : assessment of radioactive phosphorous as a marker for cellular growth. (Doctoral Dissertation). Michigan State University. Retrieved from http://etd.lib.msu.edu/islandora/object/etd:34700

Chicago Manual of Style (16^th Edition):

Bucholtz, Herbert Francis, 1943-. “Microbial protein synthesis in the rumen : assessment of radioactive phosphorous as a marker for cellular growth.” 1972. Doctoral Dissertation, Michigan State University. Accessed January 22, 2021. http://etd.lib.msu.edu/islandora/object/etd:34700.

MLA Handbook (7^th Edition):

Bucholtz, Herbert Francis, 1943-. “Microbial protein synthesis in the rumen : assessment of radioactive phosphorous as a marker for cellular growth.” 1972. Web. 22 Jan 2021.

Vancouver:

Bucholtz, Herbert Francis 1. Microbial protein synthesis in the rumen : assessment of radioactive phosphorous as a marker for cellular growth. [Internet] [Doctoral dissertation]. Michigan State University; 1972. [cited 2021 Jan 22]. Available from: http://etd.lib.msu.edu/islandora/object/etd:34700.

Council of Science Editors:

Bucholtz, Herbert Francis 1. Microbial protein synthesis in the rumen : assessment of radioactive phosphorous as a marker for cellular growth. [Doctoral Dissertation]. Michigan State University; 1972. Available from: http://etd.lib.msu.edu/islandora/object/etd:34700

30. Hadzopoulou, Margarita D. An in vitro biosynthetic study of B-glucosidase from aspergillus niger.

Degree: 1982, Texas Woman's University

URL: http://hdl.handle.net/11274/7548

Subjects/Keywords: Glucosidases – Synthesis; Proteins – Synthesis

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APA (6^th Edition):

Hadzopoulou, M. D. (1982). An in vitro biosynthetic study of B-glucosidase from aspergillus niger. (Thesis). Texas Woman's University. Retrieved from http://hdl.handle.net/11274/7548

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Hadzopoulou, Margarita D. “An in vitro biosynthetic study of B-glucosidase from aspergillus niger.” 1982. Thesis, Texas Woman's University. Accessed January 22, 2021. http://hdl.handle.net/11274/7548.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Hadzopoulou, Margarita D. “An in vitro biosynthetic study of B-glucosidase from aspergillus niger.” 1982. Web. 22 Jan 2021.

Vancouver:

Hadzopoulou MD. An in vitro biosynthetic study of B-glucosidase from aspergillus niger. [Internet] [Thesis]. Texas Woman's University; 1982. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/11274/7548.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hadzopoulou MD. An in vitro biosynthetic study of B-glucosidase from aspergillus niger. [Thesis]. Texas Woman's University; 1982. Available from: http://hdl.handle.net/11274/7548

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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